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Conserved domains on  [gi|2789581311|gb|XEN73212|]
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N-acetyltransferase family protein [Ectopseudomonas oleovorans]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-143 6.08e-17

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 72.72  E-value: 6.08e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLTPLFVKYRE-----FYNELPYPESSRKFLEKRLRRKESVIylaLADDEDKLLGFCQLYPsFSSLSL 75
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAIAegtatFETEPPSEEEREAWFAAILAPGRPVL---VAEEDGEVVGFASLGP-FRPRPA 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2789581311  76 KRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQFKN 143
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-143 6.08e-17

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 72.72  E-value: 6.08e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLTPLFVKYRE-----FYNELPYPESSRKFLEKRLRRKESVIylaLADDEDKLLGFCQLYPsFSSLSL 75
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAIAegtatFETEPPSEEEREAWFAAILAPGRPVL---VAEEDGEVVGFASLGP-FRPRPA 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2789581311  76 KRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQFKN 143
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 29-135 9.10e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 58.30  E-value: 9.10e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  29 PYPESSRKFLEKRLRRKESVIYLALADDEdkLLGFCQLYPSFSSLslkRVWILNDIYVAEDARRQLVADRLLHTAKQMAK 108
Cdd:pfam00583  15 PWPDEPLDLLEDWDEDASEGFFVAEEDGE--LVGFASLSIIDDEP---PVGEIEGLAVAPEYRGKGIGTALLQALLEWAR 89

                          90       100
                  ....*....|....*....|....*..
gi 2789581311 109 ETNAVRMRVATSRDNEVAQKVYESIGF 135
Cdd:pfam00583  90 ERGCERIFLEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-117 7.93e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.50  E-value: 7.93e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2789581311  51 LALADDEDKLLGFCQLYPSFSSlslKRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRV 117
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSG---GDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 50-144 2.06e-05

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 41.55  E-value: 2.06e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  50 YLALADDEDKLLGFcqlypSFSSLSLKRVWILNdIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKV 129
Cdd:TIGR01575  32 CYLLARIGGKVVGY-----AGVQIVLDEAHILN-IAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIAAQAL 105

                          90
                  ....*....|....*
gi 2789581311 130 YESIGFVEDEQFKNY 144
Cdd:TIGR01575 106 YKKLGFNEIAIRRNY 120
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-143 6.08e-17

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 72.72  E-value: 6.08e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLTPLFVKYRE-----FYNELPYPESSRKFLEKRLRRKESVIylaLADDEDKLLGFCQLYPsFSSLSL 75
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAIAegtatFETEPPSEEEREAWFAAILAPGRPVL---VAEEDGEVVGFASLGP-FRPRPA 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2789581311  76 KRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQFKN 143
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 1-147 1.98e-15

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 68.16  E-value: 1.98e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLTPlfvkyrefynelpypessRKFLEKRLRRKESVIYLA---LADDEDKLLGFCqlypSFSSLSLKR 77
Cdd:COG0454     1 MSIRKATPEDINFILL------------------IEALDAELKAMEGSLAGAefiAVDDKGEPIGFA----GLRRLDDKV 58

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  78 VwILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQFKNYVLP 147
Cdd:COG0454    59 L-ELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERYVAYVGG 127
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 62-144 5.98e-15

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 65.83  E-value: 5.98e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  62 GFCQLYPSFSslslKRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQF 141
Cdd:COG0456     1 GFALLGLVDG----GDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGER 76


                  ...
gi 2789581311 142 KNY 144
Cdd:COG0456    77 PNY 79
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 29-135 9.10e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 58.30  E-value: 9.10e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  29 PYPESSRKFLEKRLRRKESVIYLALADDEdkLLGFCQLYPSFSSLslkRVWILNDIYVAEDARRQLVADRLLHTAKQMAK 108
Cdd:pfam00583  15 PWPDEPLDLLEDWDEDASEGFFVAEEDGE--LVGFASLSIIDDEP---PVGEIEGLAVAPEYRGKGIGTALLQALLEWAR 89

                          90       100
                  ....*....|....*....|....*..
gi 2789581311 109 ETNAVRMRVATSRDNEVAQKVYESIGF 135
Cdd:pfam00583  90 ERGCERIFLEVAADNLAAIALYEKLGF 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 53-137 1.47e-09

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 51.69  E-value: 1.47e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  53 LADDEDKLLGFCQLYPsfssLSLKRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDnevAQKVYES 132
Cdd:pfam13508   7 VAEDDGKIVGFAALLP----LDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNR---AAAFYEK 79


                  ....*
gi 2789581311 133 IGFVE 137
Cdd:pfam13508  80 LGFEE 84
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-147 1.97e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 52.78  E-value: 1.97e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   3 IVQATLEHLDLLTPLFVKYrefynelpYPESSRKFLEKRLRRKESVIYLALADDEDKLLGFCQLYPSFSSLSLKRVWiLN 82
Cdd:COG3153     1 IRPATPEDAEAIAALLRAA--------FGPGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSPVDIDGEGPALL-LG 71

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2789581311  83 DIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATsrdNEVAQKVYESIGFVEDEQFKNYVLP 147
Cdd:COG3153    72 PLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG---DPSLLPFYERFGFRPAGELGLTLGP 133
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 1-146 7.54e-09

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 51.54  E-value: 7.54e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLTPLFV--KYREFYNELPY-PESSRKFLEKRLR---RKESVIYLALADDEDKLLGFCQLYPSfsSLS 74
Cdd:COG1670     8 LRLRPLRPEDAEALAELLNdpEVARYLPGPPYsLEEARAWLERLLAdwaDGGALPFAIEDKEDGELIGVVGLYDI--DRA 85

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2789581311  75 LKRVWIlnDIYVAEDARRQLVADRLLHTAKQMAKET-NAVRMRVATSRDNEVAQKVYESIGFVEDEQFKNYVL 146
Cdd:COG1670    86 NRSAEI--GYWLAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPDNTASIRVLEKLGFRLEGTLRDALV 156
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 1-137 4.11e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 48.83  E-value: 4.11e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311   1 MRIVQATLEHLDLLtplfvkyREFYNELPYPESSRKFLekrlrrkesviylaLADDEDKLLGFCQLYPSFsslslKRVWI 80
Cdd:COG1246     1 MTIRPATPDDVPAI-------LELIRPYALEEEIGEFW--------------VAEEDGEIVGCAALHPLD-----EDLAE 54

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2789581311  81 LNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDnevAQKVYESIGFVE 137
Cdd:COG1246    55 LRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSA---AIHFYEKLGFEE 108
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
77-147 4.90e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 47.60  E-value: 4.90e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2789581311  77 RVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKVYESIGFVEDEQFKNYVLP 147
Cdd:COG3393    14 GVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVGEYATVLFR 84
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-117 7.93e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.50  E-value: 7.93e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2789581311  51 LALADDEDKLLGFCQLYPSFSSlslKRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRV 117
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSG---GDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 38-143 1.16e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 47.65  E-value: 1.16e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  38 LEKRLRRKESVIYLAlaDDEDKLLGFCqlypsfsslSLKRVWILNDIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRV 117
Cdd:pfam13673  22 LRERIDQGEYFFFVA--FEGGQIVGVI---------ALRDRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSEL 90

                          90       100
                  ....*....|....*....|....*...
gi 2789581311 118 aTSRDNEVAQKVYESIGF--VEDEQFKN 143
Cdd:pfam13673  91 -TVNASPYAVPFYEKLGFraTGPEQEFN 117
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 50-144 2.06e-05

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 41.55  E-value: 2.06e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2789581311  50 YLALADDEDKLLGFcqlypSFSSLSLKRVWILNdIYVAEDARRQLVADRLLHTAKQMAKETNAVRMRVATSRDNEVAQKV 129
Cdd:TIGR01575  32 CYLLARIGGKVVGY-----AGVQIVLDEAHILN-IAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIAAQAL 105

                          90
                  ....*....|....*
gi 2789581311 130 YESIGFVEDEQFKNY 144
Cdd:TIGR01575 106 YKKLGFNEIAIRRNY 120
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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