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Conserved domains on  [gi|2676702273|ref|WP_332736942|]
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MlaD family protein [Flavihumibacter sp.]

Protein Classification

MlaD family protein( domain architecture ID 11444651)

MlaD family protein similar to MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane, and to mce (mammalian cell entry) proteins from Mycobacterium tuberculosis, virulence proteins required for invasion of non-phagocytic cells

Gene Ontology:  GO:0015914
PubMed:  28819315|34196044

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 31-217 4.24e-30

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 113.27  E-value: 4.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  31 KQQNLFGNKINVFADFKNIAGTREGNYVRFAGINIGTVDQIRIINDT-TVRLMLTLEKEMQpyIKADAVASIGSDGLMGD 109
Cdd:COG1463    29 GGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyRVRVTLRIDPDVP--IPADSVATIRSSGLLGE 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273 110 KLIMISPGDSATVPVKDGAVIQSVNPMDMDKVMNNISLISENA--ADLTDGLANIVGRIDDGEGSLGRLLND-DKLAVKL 186
Cdd:COG1463   107 KYVELSPGGDSGPPLADGDVIPLESPVDLEDLLSQLSPLLEALdpGALLDNLNTLTDTLADAAAQLDALLDNlNTLSDTL 186

                         170       180       190
                  ....*....|....*....|....*....|.
gi 2676702273 187 EGAIDQTKQTVGTIKKTASTANETLEAAQGS 217
Cdd:COG1463   187 AERRDDLRALLASLNALSAQLDGLLADNRGT 217
 
Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 31-217 4.24e-30

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 113.27  E-value: 4.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  31 KQQNLFGNKINVFADFKNIAGTREGNYVRFAGINIGTVDQIRIINDT-TVRLMLTLEKEMQpyIKADAVASIGSDGLMGD 109
Cdd:COG1463    29 GGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyRVRVTLRIDPDVP--IPADSVATIRSSGLLGE 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273 110 KLIMISPGDSATVPVKDGAVIQSVNPMDMDKVMNNISLISENA--ADLTDGLANIVGRIDDGEGSLGRLLND-DKLAVKL 186
Cdd:COG1463   107 KYVELSPGGDSGPPLADGDVIPLESPVDLEDLLSQLSPLLEALdpGALLDNLNTLTDTLADAAAQLDALLDNlNTLSDTL 186

                         170       180       190
                  ....*....|....*....|....*....|.
gi 2676702273 187 EGAIDQTKQTVGTIKKTASTANETLEAAQGS 217
Cdd:COG1463   187 AERRDDLRALLASLNALSAQLDGLLADNRGT 217
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 38-117 1.03e-11

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 59.13  E-value: 1.03e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  38 NKINVFADFKNIAGTREGNYVRFAGINIGTVDQIRIINDT-TVRLMLTLEKEMQPYIKADAVASIGSDGLMGDKLIMISP 116
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGkGVRVTLRIDPDYDVLLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 2676702273 117 G 117
Cdd:pfam02470  81 G 81
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 42-131 2.36e-11

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 60.18  E-value: 2.36e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  42 VFADFKNIAGTREGNYVRFAGINIGTVDQIRiINDTTVRLMLTLEKEMQPYIKADAVASIGSDGLMGDKLIMISPGdSAT 121
Cdd:TIGR04430  41 VTASFDNIGGLKVGAPVKIAGVKVGRVTDIS-LDPKTYQAVVTLSIDKDYKLPDDSSASILTSGLLGEQYIGLSPG-GDE 118

                          90
                  ....*....|
gi 2676702273 122 VPVKDGAVIQ 131
Cdd:TIGR04430 119 DYLKDGDEIE 128
 
Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 31-217 4.24e-30

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 113.27  E-value: 4.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  31 KQQNLFGNKINVFADFKNIAGTREGNYVRFAGINIGTVDQIRIINDT-TVRLMLTLEKEMQpyIKADAVASIGSDGLMGD 109
Cdd:COG1463    29 GGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyRVRVTLRIDPDVP--IPADSVATIRSSGLLGE 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273 110 KLIMISPGDSATVPVKDGAVIQSVNPMDMDKVMNNISLISENA--ADLTDGLANIVGRIDDGEGSLGRLLND-DKLAVKL 186
Cdd:COG1463   107 KYVELSPGGDSGPPLADGDVIPLESPVDLEDLLSQLSPLLEALdpGALLDNLNTLTDTLADAAAQLDALLDNlNTLSDTL 186

                         170       180       190
                  ....*....|....*....|....*....|.
gi 2676702273 187 EGAIDQTKQTVGTIKKTASTANETLEAAQGS 217
Cdd:COG1463   187 AERRDDLRALLASLNALSAQLDGLLADNRGT 217
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 38-117 1.03e-11

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 59.13  E-value: 1.03e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  38 NKINVFADFKNIAGTREGNYVRFAGINIGTVDQIRIINDT-TVRLMLTLEKEMQPYIKADAVASIGSDGLMGDKLIMISP 116
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGkGVRVTLRIDPDYDVLLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 2676702273 117 G 117
Cdd:pfam02470  81 G 81
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 42-131 2.36e-11

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 60.18  E-value: 2.36e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  42 VFADFKNIAGTREGNYVRFAGINIGTVDQIRiINDTTVRLMLTLEKEMQPYIKADAVASIGSDGLMGDKLIMISPGdSAT 121
Cdd:TIGR04430  41 VTASFDNIGGLKVGAPVKIAGVKVGRVTDIS-LDPKTYQAVVTLSIDKDYKLPDDSSASILTSGLLGEQYIGLSPG-GDE 118

                          90
                  ....*....|
gi 2676702273 122 VPVKDGAVIQ 131
Cdd:TIGR04430 119 DYLKDGDEIE 128
Mtu_fam_mce TIGR00996
virulence factor Mce family protein; Members of this paralogous family are found as six tandem ...
 42-239 7.72e-11

virulence factor Mce family protein; Members of this paralogous family are found as six tandem homologous proteins in the same orientation per cassette, in four separate cassettes in Mycobacterium tuberculosis. The six members of each cassette represent six subfamilies. One subfamily includes the protein mce (mycobacterial cell entry), a virulence protein required for invasion of non-phagocytic cells. [Cellular processes, Pathogenesis]


Pssm-ID: 273384 [Multi-domain]  Cd Length: 291  Bit Score: 60.76  E-value: 7.72e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273  42 VFADFKNIAGTREGNYVRFAGINIGTVDQIrIINDTTVRLMLTLEKEMqpYIKADAVASIGSDGLMGDKLI-MISPGDSA 120
Cdd:TIGR00996  35 VVAYFTDAGGLYPGDKVRVAGVPVGKVTAI-EPRGARARVTFSLDRSV--TLPANATAAIRTTTLLGSRYIeLTPPGGPP 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2676702273 121 TVPVKDGAVIQSVN---PMDMDKVMNNIS-------------LISENA----------ADLTDGLANIVGRIDDGEGSLG 174
Cdd:TIGR00996 112 GGRLSDGGTIPLSRttvPLELDDLLGSLTrllngldpeklnaILNALAqalagqgpqlRNLLDGLAQLTAALNERDGDIG 191

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2676702273 175 RLLndDKLAVkLEGAIDQTKQTVGTIKKTASTANETLeaaqgsfllrghfkRKERQRIKDSIANA 239
Cdd:TIGR00996 192 ELI--RNLNR-VLDVLADRSDQLDRLLDNLPTLIATL--------------ADRSDALDDALAAL 239
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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