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Conserved domains on  [gi|2562027156|ref|WP_305562390|]
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tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Limnobacter sp.]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-156 5.91e-101

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 286.16  E-value: 5.91e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDpqRCFAM 80
Cdd:COG0219     1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGG--RLFLF 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFArENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEGGA 156
Cdd:COG0219    79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPA-DQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-156 5.91e-101

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 286.16  E-value: 5.91e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDpqRCFAM 80
Cdd:COG0219     1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGG--RLFLF 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFArENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEGGA 156
Cdd:COG0219    79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPA-DQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-150 5.10e-88

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 253.14  E-value: 5.10e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   3 HVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDtcKPDPQRCFAMTT 82
Cdd:cd18094     1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLE--ALPPGRLFLFTT 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2562027156  83 KGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFaRENWLRLPMRPESRSLNLSNSVAVMVYEAWRQL 150
Cdd:cd18094    79 KGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAF-PDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-154 4.01e-76

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 223.72  E-value: 4.01e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTckPDPQRCFAM 80
Cdd:PRK10358    1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEA--ENPQRLFAL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFARENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEG 154
Cdd:PRK10358   79 TTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPG 152
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 3.14e-73

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 216.13  E-value: 3.14e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKpdPQRCFAM 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK--PQKLFAL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFArENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEG 154
Cdd:TIGR00185  79 TKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNLM-EQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPG 151
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-144 4.39e-43

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 139.20  E-value: 4.39e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGV-QLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCFA 79
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2562027156  80 MTTKGTrSPFEVQFKAGDYLMFGSETRGLSEDVRNwfARENWLRLPMRPESRSLNLSNSVAVMVY 144
Cdd:pfam00588  81 TSLGGK-SLYEIDFPKKVALVFGNEGTGLSNEVLE--LCDRLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-156 5.91e-101

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 286.16  E-value: 5.91e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDpqRCFAM 80
Cdd:COG0219     1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGG--RLFLF 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFArENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEGGA 156
Cdd:COG0219    79 TTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAAPA-DQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-150 5.10e-88

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 253.14  E-value: 5.10e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   3 HVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDtcKPDPQRCFAMTT 82
Cdd:cd18094     1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLE--ALPPGRLFLFTT 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2562027156  83 KGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFaRENWLRLPMRPESRSLNLSNSVAVMVYEAWRQL 150
Cdd:cd18094    79 KGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAF-PDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-154 4.01e-76

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 223.72  E-value: 4.01e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTckPDPQRCFAM 80
Cdd:PRK10358    1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEA--ENPQRLFAL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFARENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEG 154
Cdd:PRK10358   79 TTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPG 152
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 3.14e-73

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 216.13  E-value: 3.14e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKpdPQRCFAM 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEK--PQKLFAL 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2562027156  81 TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFArENWLRLPMRPESRSLNLSNSVAVMVYEAWRQLGFEG 154
Cdd:TIGR00185  79 TKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNLM-EQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPG 151
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-144 4.39e-43

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 139.20  E-value: 4.39e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGV-QLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCFA 79
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2562027156  80 MTTKGTrSPFEVQFKAGDYLMFGSETRGLSEDVRNwfARENWLRLPMRPESRSLNLSNSVAVMVY 144
Cdd:pfam00588  81 TSLGGK-SLYEIDFPKKVALVFGNEGTGLSNEVLE--LCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 3-146 5.84e-31

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 108.47  E-value: 5.84e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   3 HVVLVEPEIPPNTGNVIRLCANTGV-QLHLIEPLGFPlEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCFA-M 80
Cdd:cd18082     1 HLVLDNIRSPGNIGSIFRTADAFGVeKIYLVGPTADI-FDPKAKRTSRGTHFSVPYERVDNFEEYLKSLKEEGYPLIGlE 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2562027156  81 TTKGTRSPFEVQFKA--GDYLMFGSETRGLSEDVRNwFARENwLRLPMRPESRSLNLSNSVAVMVYEA 146
Cdd:cd18082    80 LTDGSIPLSEFKFKKpkKIALIFGNERSGLSPELLA-LCDYT-VHIPMSGKNSSLNVSVATGIALYEF 145
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 1-154 1.84e-16

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 73.23  E-value: 1.84e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   1 MIHVVLVEPEIPPNTGNVIRLCANTGV-QLHLIEPLGFPLEDARMRRAG----LDyheyaRMQVHAGWQAFLDTCkpdpQ 75
Cdd:COG0565     3 NIRVVLVEPQHPGNIGAAARAMKNFGLsDLRLVNPRGWPDEEARAMASGaddvLE-----NARVVDTLEEALADC----D 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156  76 RCFAMT--TKGTRSPF--------EVQFKAGDY---LMFGSETRGLS-EDVrnwfARENWL-RLPMRPESRSLNLSNSVA 140
Cdd:COG0565    74 LVVGTTarPRELRKPVltpreaaeELLARAAGGkvaLVFGRERSGLTnEEL----ALCDALvTIPTNPEYPSLNLAQAVQ 149

                         170
                  ....*....|....
gi 2562027156 141 VMVYEaWRQLGFEG 154
Cdd:COG0565   150 VVAYE-LRKAALEA 162
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 2-145 2.25e-15

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 68.68  E-value: 2.25e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   2 IHVVLVEPEIPPNTGNVIRLCANTGV-QLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCkpdpQRCFAM 80
Cdd:cd18093     1 IRVVLVEPQHPGNIGAVARAMKNFGLsDLRLVNPRCDDPEEAARALASGAEDVLENAKVYDTLEEALADC----DLVVAT 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156  81 T---TKGTRSPFEVQFKAGDY----------LMFGSETRGLS-EDVR--NWFarenwLRLPMRPESRSLNLSNSVAVMVY 144
Cdd:cd18093    77 TarpRGLRRPLLTPRELAELLkehaaggkvaLVFGREDSGLTnEELAlcDLL-----VTIPTNPEYPSLNLAQAVAIVLY 151


                  .
gi 2562027156 145 E 145
Cdd:cd18093   152 E 152
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 78-149 9.74e-12

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 59.13  E-value: 9.74e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2562027156  78 FAMTTKGTRSPFEVQFKAGDYLMFGSETRGLSEDvrnWFAREN-WLRLPMRPESRSLNLSNSVAVMVYEAWRQ 149
Cdd:cd18104    76 LATTPKAEKLYYEADLRGPIAIVVGSESEGLSEF---WLKAADeRVRIPMAGQADSLNVSVAAAILLYEALRQ 145
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 4-149 5.85e-11

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 58.67  E-value: 5.85e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   4 VVLVEPEIPPNTGNVIRLCANTGVQLHLIEPLGFPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCFAMTTK 83
Cdd:COG0566   118 LVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKVVRASAGALFHVPVARVTNLADALEELKEAGFWVVGTDLD 197

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2562027156  84 GTRSPFEVQFKAGDYLMFGSETRGLSEDVRnwfarEN---WLRLPMRPESRSLNLSNSVAVMVYEAWRQ 149
Cdd:COG0566   198 GEASLYEVDLPGPVALVLGNEGKGLSRLVL-----EAcdeLVRIPMAGGVESLNVAVAAAILLYEAARQ 261
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 2-146 5.37e-09

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 51.80  E-value: 5.37e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   2 IHVVLVEPEIPPNTGNVIRLCANTGV-QLHL--IEPLgfPlEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCF 78
Cdd:cd18097     1 LVVVLDNLRSAHNVGSIFRTADAAGVeKIYLcgYTPT--P-PHPKVAKTALGAEESVPWEHFEDTLEAVRALKARGYTII 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2562027156  79 AM-TTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNwfARENWLRLPMRPESRSLNLSNSVAVMVYEA 146
Cdd:cd18097    78 ALeTTENSVPLHEARLPRPLALVLGNEVRGVSPEVLA--LCDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 52-146 8.09e-09

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 51.27  E-value: 8.09e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156  52 HEYARMQVHAGWQAFLDTCKPDPQRCFAMTTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNWFARenWLRLPMRPESR 131
Cdd:cd18095    51 LFRLPIIRVDDLEELLEELKEAGYKVVAADLDGGSSLYDLDLPKPVALVLGNEGNGLSDELLELCDK--RVTIPMSGGVE 128

                          90
                  ....*....|....*
gi 2562027156 132 SLNLSNSVAVMVYEA 146
Cdd:cd18095   129 SLNVAVAAAILLYEL 143
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 4-150 5.84e-07

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 46.31  E-value: 5.84e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156   4 VVLVEPEIPPNTGNVIRLCANTGVQ-LHLIEPlgfPLEDARMRRAGLDYHEYARMQVHAGWQAFLDTCKPDPQRCFAMTT 82
Cdd:cd18092    19 VVLENVHDPHNASAVLRTCDAFGVQdVHVIEP---KNKFKKNRGVAMGADKWLDVHRHDSTEECLAALKEQGYRIVATSL 95

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2562027156  83 KGTRSPFEvqfkAGDY-----LMFGSETRGLSEDVRNwfARENWLRLPMRPESRSLNLSNSVAVMVYEAWRQL 150
Cdd:cd18092    96 SPDAVPLD----ELDYtrptaLVFGNEKDGVSEEALA--AADERVYIPMYGFVESLNVSVAAAIILYELQRQR 162
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 77-146 2.61e-06

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 44.30  E-value: 2.61e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2562027156  77 CFAMTTKGTRSPFEVQFKAGDYLMFGSETRGLSEDVRNwFARENwLRLPMRPESRSLNLSNSVAVMVYEA 146
Cdd:cd18103    76 IYGADGEAEKSLYDVDLTGPLALVLGSEGKGLRRLVLK-NCDFL-VKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 99-146 1.13e-04

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 39.93  E-value: 1.13e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 2562027156  99 LMFGSETRGLSEDVRNWFARENWLRL--PMRPESRSLNLSNSVAVMVYEA 146
Cdd:cd18106    99 LVIGGETHGVSPEAYKLAHSTGGSRVhiPLANGVDSLNSAVAASILLFEM 148
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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