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Conserved domains on  [gi|2527307910|ref|WP_288333697|]
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UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Cloacibacillus porcorum]

Protein Classification

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase( domain architecture ID 11433680)

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

CATH:  3.90.190.20|3.40.1190.10|3.40.1390.10
EC:  6.3.2.10
Gene Ontology:  GO:0005524|GO:0009252|GO:0047480|GO:0071555
PubMed:  16934839
SCOP:  4000234

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 10-463 2.25e-134

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


:

Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 394.85  E-value: 2.25e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  10 AALCGGRLYGPDIKISRGWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLaeaaelerlnIRGEAYSGVSFI 89
Cdd:COG0770     9 AEATGGRLIGDPDLVVTGVSTDSRKIKPGDLFVALKGERFDGHDFVAQALAKGAAAAL----------VSRPLPADLPLI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  90 AVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLE 169
Cdd:COG0770    79 VVDDTLKALQQLAAAHRARFNIPVIAITGSNGKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 170 LGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEIC-GSPRLAAVVFNNDNCLLKNYMSYNfdNIKKI 248
Cdd:COG0770   159 MGMNHPGEIAYLARIARPDIAVITNIGPAHLEGFGSLEGIARAKGEIFeGLPPGGVAVLNADDPLLAALAERA--KARVL 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 249 VVGSGEGASLTIKeaEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPIS 328
Cdd:COG0770   237 TFGLSEDADVRAE--DIELDEDG--TRFTLHTPGGELEVTLPLPGRHNVSNALAAAAVALALGLDLEEIAAGLAAFQPVK 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 329 GRGLCKRSASRGWVIDEAYNANPASMSAAIRNTrAAAESLGvRKWAILAGMRELGESAAEWHRRiIGELA---DFDGVML 405
Cdd:COG0770   313 GRLEVIEGAGGVTLIDDSYNANPDSMKAALDVL-AQLPGGG-RRIAVLGDMLELGEESEELHRE-VGELAaelGIDRLFT 389

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 406 LGEEWRGC--GPLPEGAALFSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVVGALTEA 463
Cdd:COG0770   390 VGELARAIaeAAGGERAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEG 449
 
Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 10-463 2.25e-134

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 394.85  E-value: 2.25e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  10 AALCGGRLYGPDIKISRGWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLaeaaelerlnIRGEAYSGVSFI 89
Cdd:COG0770     9 AEATGGRLIGDPDLVVTGVSTDSRKIKPGDLFVALKGERFDGHDFVAQALAKGAAAAL----------VSRPLPADLPLI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  90 AVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLE 169
Cdd:COG0770    79 VVDDTLKALQQLAAAHRARFNIPVIAITGSNGKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 170 LGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEIC-GSPRLAAVVFNNDNCLLKNYMSYNfdNIKKI 248
Cdd:COG0770   159 MGMNHPGEIAYLARIARPDIAVITNIGPAHLEGFGSLEGIARAKGEIFeGLPPGGVAVLNADDPLLAALAERA--KARVL 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 249 VVGSGEGASLTIKeaEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPIS 328
Cdd:COG0770   237 TFGLSEDADVRAE--DIELDEDG--TRFTLHTPGGELEVTLPLPGRHNVSNALAAAAVALALGLDLEEIAAGLAAFQPVK 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 329 GRGLCKRSASRGWVIDEAYNANPASMSAAIRNTrAAAESLGvRKWAILAGMRELGESAAEWHRRiIGELA---DFDGVML 405
Cdd:COG0770   313 GRLEVIEGAGGVTLIDDSYNANPDSMKAALDVL-AQLPGGG-RRIAVLGDMLELGEESEELHRE-VGELAaelGIDRLFT 389

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 406 LGEEWRGC--GPLPEGAALFSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVVGALTEA 463
Cdd:COG0770   390 VGELARAIaeAAGGERAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEG 449
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 31-460 7.18e-96

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 294.94  E-value: 7.18e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  31 DSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELERLnirgeaysGVSFIAVADTQRALASLAEEYLRRVS 110
Cdd:TIGR01143   2 DSRAIKPGDLFIALKGERFDGHDFVEQALAAGAVAVVVDREVGPDN--------GLPQILVDDTLEALQALARAKRAKFS 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 111 PRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLELGTNHFGEIEEMVSHFPPEIA 190
Cdd:TIGR01143  74 GKVIGITGSSGKTTTKEMLAAILSHKYKVFATPGNFNNEIGLPLTLLRAPGDHDYAVLEMGASHPGEIAYLAEIAKPDIA 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 191 VITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYMSYNFDniKKIVVGSGEGAslTIKEAEVRLDE 269
Cdd:TIGR01143 154 VITNIGPAHLEGFGSLEGIAEAKGEIlQGLKENGIAVINADDPAFADLAKRLPN--RNILSFGFEGG--DFVAKDISYSA 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 270 NGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPISGRG---LCKRSAsrgwVIDEA 346
Cdd:TIGR01143 230 LG-STSFTLVAPGGEFEVSLPLLGRHNVMNALAAAALALELGIPLEEIAEGLAELKLVKGRFevqTKNGLT----LIDDT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 347 YNANPASMSAAIRntraAAESLGVRKWAILAGMRELGESAAEWHRRiIGELA---DFDGVMLLGEEWRGC-GPLPEGAAL 422
Cdd:TIGR01143 305 YNANPDSMRAALD----ALARFPGKKILVLGDMAELGEYSEELHAE-VGRYAnslGIDLVFLVGEEAAVIyDSFGKQGKH 379

                         410       420       430
                  ....*....|....*....|....*....|....*...
gi 2527307910 423 FSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVVGAL 460
Cdd:TIGR01143 380 FADKDELLAFLKTLVRKGDVVLVKGSRSVKLEKVVEAL 417
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 7-460 1.71e-68

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 235.37  E-value: 1.71e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910   7 SEGAALCGGRLYGP---DIKISRGwKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEaaelerlniRGEAY 83
Cdd:PRK11929  506 IKLRAAKGGSNSLQinsLPHAGAV-STDSRSVGRGELFVALRGENFDGHDYLPQAFAAGACAAVVE---------RQVAD 575

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  84 SGVSFIAVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAAL---KNRFRVHSAIRSFNTLVGCSLTILAMA 160
Cdd:PRK11929  576 VDLPQIVVDDTRAALGRLATAWRARFSLPVVAITGSNGKTTTKEMIAAILaawQGEDRVLATEGNFNNEIGVPLTLLRLR 655

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 161 EDTEVLVLELGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYMS 239
Cdd:PRK11929  656 AQHRAAVFELGMNHPGEIAYLAAIAAPTVALVTNAQREHQEFMHSVEAVARAKGEIiAALPEDGVAVVNGDDPYTAIWAK 735

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 240 -YNFDNIKKIVVGSGEGASLTIKEAEVRLDENGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAA 318
Cdd:PRK11929  736 lAGARRVLRFGLQPGADVYAEKIAKDISVGEAG-GTRCQVVTPAGSAEVYLPLIGEHNLRNALAAIACALAAGASLKQIR 814

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 319 AGIAEISPISGRGLCKRSASRGWVIDEAYNANPASMSAAIRNTraaAESLGVRKWAILAGMRELGESAAEWHRRIIGELA 398
Cdd:PRK11929  815 AGLERFQPVAGRMQRRRLSCGTRIIDDTYNANPDSMRAAIDVL---AELPNGPRALVLGDMLELGDNGPAMHREVGKYAR 891

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2527307910 399 D--FDGVMLLGEEWR--GCGPLPEGAALFSSLEELISKI-DYNDLNDkMILVKGSNSYGLKRVVGAL 460
Cdd:PRK11929  892 QlgIDALITLGEAARdaAAAFGAGARGVCASVDEIIAALrGALPEGD-SVLIKGSRFMRLERVVDAL 957
Mur_ligase_M pfam08245
Mur ligase middle domain;
116-307 1.53e-26

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 106.23  E-value: 1.53e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 116 ITGSVGKTTTRELTAAALKNRFRVHS--------AIRSFNTLVGCSLTILAMAE-DTEVLVLELGTNHFGEiEEMVSHFP 186
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGtigtyigkSGNTTNNAIGLPLTLAEMVEaGAEYAVLEVSSHGLGE-GRLSGLLK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 187 PEIAVITEVAPCHLEGFGSLEGVLRAKLEICGSPRL-AAVVFNNDNcllkNYMSYNFDNIKKI---VVGSGEGASLTIKE 262
Cdd:pfam08245  80 PDIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEdGIAVINADD----PYGAFLIAKLKKAgvrVITYGIEGEADLRA 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2527307910 263 AEVRLDENGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVA 307
Cdd:pfam08245 156 ANIELSSDG-TSFDLFTVPGGELEIEIPLLGRHNVYNALAAIAAA 199
 
Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 10-463 2.25e-134

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 394.85  E-value: 2.25e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  10 AALCGGRLYGPDIKISRGWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLaeaaelerlnIRGEAYSGVSFI 89
Cdd:COG0770     9 AEATGGRLIGDPDLVVTGVSTDSRKIKPGDLFVALKGERFDGHDFVAQALAKGAAAAL----------VSRPLPADLPLI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  90 AVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLE 169
Cdd:COG0770    79 VVDDTLKALQQLAAAHRARFNIPVIAITGSNGKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 170 LGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEIC-GSPRLAAVVFNNDNCLLKNYMSYNfdNIKKI 248
Cdd:COG0770   159 MGMNHPGEIAYLARIARPDIAVITNIGPAHLEGFGSLEGIARAKGEIFeGLPPGGVAVLNADDPLLAALAERA--KARVL 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 249 VVGSGEGASLTIKeaEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPIS 328
Cdd:COG0770   237 TFGLSEDADVRAE--DIELDEDG--TRFTLHTPGGELEVTLPLPGRHNVSNALAAAAVALALGLDLEEIAAGLAAFQPVK 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 329 GRGLCKRSASRGWVIDEAYNANPASMSAAIRNTrAAAESLGvRKWAILAGMRELGESAAEWHRRiIGELA---DFDGVML 405
Cdd:COG0770   313 GRLEVIEGAGGVTLIDDSYNANPDSMKAALDVL-AQLPGGG-RRIAVLGDMLELGEESEELHRE-VGELAaelGIDRLFT 389

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 406 LGEEWRGC--GPLPEGAALFSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVVGALTEA 463
Cdd:COG0770   390 VGELARAIaeAAGGERAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEG 449
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 31-460 7.18e-96

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 294.94  E-value: 7.18e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  31 DSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELERLnirgeaysGVSFIAVADTQRALASLAEEYLRRVS 110
Cdd:TIGR01143   2 DSRAIKPGDLFIALKGERFDGHDFVEQALAAGAVAVVVDREVGPDN--------GLPQILVDDTLEALQALARAKRAKFS 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 111 PRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLELGTNHFGEIEEMVSHFPPEIA 190
Cdd:TIGR01143  74 GKVIGITGSSGKTTTKEMLAAILSHKYKVFATPGNFNNEIGLPLTLLRAPGDHDYAVLEMGASHPGEIAYLAEIAKPDIA 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 191 VITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYMSYNFDniKKIVVGSGEGAslTIKEAEVRLDE 269
Cdd:TIGR01143 154 VITNIGPAHLEGFGSLEGIAEAKGEIlQGLKENGIAVINADDPAFADLAKRLPN--RNILSFGFEGG--DFVAKDISYSA 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 270 NGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPISGRG---LCKRSAsrgwVIDEA 346
Cdd:TIGR01143 230 LG-STSFTLVAPGGEFEVSLPLLGRHNVMNALAAAALALELGIPLEEIAEGLAELKLVKGRFevqTKNGLT----LIDDT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 347 YNANPASMSAAIRntraAAESLGVRKWAILAGMRELGESAAEWHRRiIGELA---DFDGVMLLGEEWRGC-GPLPEGAAL 422
Cdd:TIGR01143 305 YNANPDSMRAALD----ALARFPGKKILVLGDMAELGEYSEELHAE-VGRYAnslGIDLVFLVGEEAAVIyDSFGKQGKH 379

                         410       420       430
                  ....*....|....*....|....*....|....*...
gi 2527307910 423 FSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVVGAL 460
Cdd:TIGR01143 380 FADKDELLAFLKTLVRKGDVVLVKGSRSVKLEKVVEAL 417
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 7-460 1.71e-68

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 235.37  E-value: 1.71e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910   7 SEGAALCGGRLYGP---DIKISRGwKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEaaelerlniRGEAY 83
Cdd:PRK11929  506 IKLRAAKGGSNSLQinsLPHAGAV-STDSRSVGRGELFVALRGENFDGHDYLPQAFAAGACAAVVE---------RQVAD 575

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  84 SGVSFIAVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAAL---KNRFRVHSAIRSFNTLVGCSLTILAMA 160
Cdd:PRK11929  576 VDLPQIVVDDTRAALGRLATAWRARFSLPVVAITGSNGKTTTKEMIAAILaawQGEDRVLATEGNFNNEIGVPLTLLRLR 655

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 161 EDTEVLVLELGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYMS 239
Cdd:PRK11929  656 AQHRAAVFELGMNHPGEIAYLAAIAAPTVALVTNAQREHQEFMHSVEAVARAKGEIiAALPEDGVAVVNGDDPYTAIWAK 735

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 240 -YNFDNIKKIVVGSGEGASLTIKEAEVRLDENGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAA 318
Cdd:PRK11929  736 lAGARRVLRFGLQPGADVYAEKIAKDISVGEAG-GTRCQVVTPAGSAEVYLPLIGEHNLRNALAAIACALAAGASLKQIR 814

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 319 AGIAEISPISGRGLCKRSASRGWVIDEAYNANPASMSAAIRNTraaAESLGVRKWAILAGMRELGESAAEWHRRIIGELA 398
Cdd:PRK11929  815 AGLERFQPVAGRMQRRRLSCGTRIIDDTYNANPDSMRAAIDVL---AELPNGPRALVLGDMLELGDNGPAMHREVGKYAR 891

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2527307910 399 D--FDGVMLLGEEWR--GCGPLPEGAALFSSLEELISKI-DYNDLNDkMILVKGSNSYGLKRVVGAL 460
Cdd:PRK11929  892 QlgIDALITLGEAARdaAAAFGAGARGVCASVDEIIAALrGALPEGD-SVLIKGSRFMRLERVVDAL 957
murF PRK10773
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed
 10-462 4.00e-54

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed


Pssm-ID: 182718 [Multi-domain]  Cd Length: 453  Bit Score: 187.55  E-value: 4.00e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  10 AALCGGRLYGPDIKISRgWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAaeleRLNIrgeaysGVSFI 89
Cdd:PRK10773   10 ADILNGELQGADITIDA-VTTDTRKVTPGCLFVALKGERFDAHDFADDAKAAGAGALLVSR----PLDI------DLPQL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  90 AVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLE 169
Cdd:PRK10773   79 VVKDTRLAFGQLAAWVRQQVPARVVALTGSSGKTSVKEMTAAILRQCGNTLYTAGNLNNDIGVPLTLLRLTPEHDYAVIE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 170 LGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYMSYNFDniKKI 248
Cdd:PRK10773  159 LGANHQGEIAYTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIfSGLPENGIAIMNADSNDWLNWQSVIGS--KTV 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 249 VVGSGEGA-SLTIKEAEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPI 327
Cdd:PRK10773  237 WRFSPNAAnSVDFTATNIHVTSHG--TEFTLHTPTGSVDVLLPLPGRHNIANALAAAALAMSVGATLDAVKAGLANLKAV 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 328 SGRGLCKRSASRGWVIDEAYNANPASMSAAIrntRAAAESLGVRKwAILAGMRELGESAAEWHRRiIGE---LADFDGVM 404
Cdd:PRK10773  315 PGRLFPIQLAEGQLLLDDSYNANVGSMTAAA---QVLAEMPGYRV-MVVGDMAELGAESEACHRQ-VGEaakAAGIDKVL 389

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2527307910 405 LLGE------EWRGCGP-LPEGAALFSSLEELISKidYNDLNdkmILVKGSNSYGLKRVVGALTE 462
Cdd:PRK10773  390 SVGKlshaisEASGVGEhFADKTALIARLKALLAE--HQVIT---ILVKGSRSAAMEEVVRALQE 449
PRK14093 PRK14093
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase; ...
 2-462 2.46e-53

UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase; Provisional


Pssm-ID: 184501 [Multi-domain]  Cd Length: 479  Bit Score: 186.13  E-value: 2.46e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910   2 ALFCASEGAALCGGRLYGPDIKISRGWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAElerlniRGE 81
Cdd:PRK14093    5 PLWTSDAMAEAMGATRSGALPRDVTGISIDSRTLAPGDAYFAIKGDVHDGHAFVAAALKAGAALAVVERAQ------RDK 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  82 AYSGVSFIAVADTQRALASLAEEYLRRVSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAE 161
Cdd:PRK14093   79 FAADAPLLVVDDVLAALRDLGRAARARLEAKVIAVTGSVGKTSTKEALRGVLGAQGETHASVASFNNHWGVPLSLARCPA 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 162 DTEVLVLELGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNNDNCLLKNYM-S 239
Cdd:PRK14093  159 DARFAVFEIGMNHAGEIEPLVKMVRPHVAIITTVEPVHLEFFSGIEAIADAKAEIfTGLEPGGAAVLNRDNPQFDRLAaS 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 240 YNFDNIKKIV-VGSGEGASLTIKEAEVRldengPRTSAVLCR-EGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEA 317
Cdd:PRK14093  239 ARAAGIARIVsFGADEKADARLLDVALH-----ADCSAVHADiLGHDVTYKLGMPGRHIAMNSLAVLAAAELAGADLALA 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 318 AAGIAEISPISGRGLCKRSASRG---WVIDEAYNANPASMSAAIRNTRAAAESLGVRKWAILAGMRELGESAAEWHRRII 394
Cdd:PRK14093  314 ALALSQVQPAAGRGVRHTLEVGGgeaTLIDESYNANPASMAAALGVLGRAPVGPQGRRIAVLGDMLELGPRGPELHRGLA 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 395 GEL--ADFDGVMLlgeewrgCGPL-----------------PEGAALFSSLEELISKIDyndlndkMILVKGSNSYGLKR 455
Cdd:PRK14093  394 EAIraNAIDLVFC-------CGPLmrnlwdalssgkrggyaEDAAALESQVVAAIRAGD-------VIMVKGSLGSRMKT 459


                  ....*..
gi 2527307910 456 VVGALTE 462
Cdd:PRK14093  460 IVTALEK 466
PRK11930 PRK11930
putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine ...
 31-457 1.35e-44

putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase; Provisional


Pssm-ID: 237026 [Multi-domain]  Cd Length: 822  Bit Score: 166.67  E-value: 1.35e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  31 DSREI-NPGDG-FVALKGAATDGHLYLHQAIERGAKLLLAEAaelerLNIRGEAYSGVSFIAVADTQRALASLAEEYLRR 108
Cdd:PRK11930   30 DSRSLsFPENTlFFALKGERNDGHRYIQELYEKGVRNFVVSE-----EKHPEESYPDANFLKVKDPLKALQELAAYHRSQ 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 109 VSPRVAAITGSVGKTTTRELTAAALKNRFRVHSAIRSFNTLVGCSLTILAMAEDTEVLVLELGTNHFGEIEEMVSHFPPE 188
Cdd:PRK11930  105 FDIPVIGITGSNGKTIVKEWLYQLLSPDYNIVRSPRSYNSQIGVPLSVWQLNEEHELGIFEAGISQPGEMEALQKIIKPT 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 189 IAVITEVAPCHLEGFGSLEGVLRAKLEIcgSPRLAAVVFNNDNCLLKNYMSYNFDNIKKIVvgsgegASLTIKEAEVRLD 268
Cdd:PRK11930  185 IGILTNIGGAHQENFRSIKQKIMEKLKL--FKDCDVIIYNGDNELISSCITKSNLTLKLIS------WSRKDPEAPLYIP 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 269 --ENGPRTSAVLCR-EGKDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPISGRGLCKRSASRGWVIDE 345
Cdd:PRK11930  257 fvEKKEDHTVISYTyKGEDFHFEIPFIDDASIENLIHCIAVLLYLGYSADQIQERMARLEPVAMRLEVKEGINNCTLIND 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 346 AYNANPASMSAAIRNTRAAAESLgvRKWAILAGMRELGESAAEWHRRiIGEL---ADFDGVMLLGEEWRGCGPL--PEGA 420
Cdd:PRK11930  337 SYNSDLQSLDIALDFLNRRSQSK--KKTLILSDILQSGQSPEELYRK-VAQLiskRGIDRLIGIGEEISSEASKfeGTEK 413

                         410       420       430
                  ....*....|....*....|....*....|....*..
gi 2527307910 421 ALFSSLEELISKIDYNDLNDKMILVKGSNSYGLKRVV 457
Cdd:PRK11930  414 EFFKTTEAFLKSFAFLKFRNELILVKGARKFEFEQIT 450
Mur_ligase_M pfam08245
Mur ligase middle domain;
116-307 1.53e-26

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 106.23  E-value: 1.53e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 116 ITGSVGKTTTRELTAAALKNRFRVHS--------AIRSFNTLVGCSLTILAMAE-DTEVLVLELGTNHFGEiEEMVSHFP 186
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGtigtyigkSGNTTNNAIGLPLTLAEMVEaGAEYAVLEVSSHGLGE-GRLSGLLK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 187 PEIAVITEVAPCHLEGFGSLEGVLRAKLEICGSPRL-AAVVFNNDNcllkNYMSYNFDNIKKI---VVGSGEGASLTIKE 262
Cdd:pfam08245  80 PDIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEdGIAVINADD----PYGAFLIAKLKKAgvrVITYGIEGEADLRA 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2527307910 263 AEVRLDENGpRTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVA 307
Cdd:pfam08245 156 ANIELSSDG-TSFDLFTVPGGELEIEIPLLGRHNVYNALAAIAAA 199
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 30-330 2.05e-26

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 110.94  E-value: 2.05e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  30 CDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELerlnirgEAYSGVSFIAVADTQRALASLAEEYLRRV 109
Cdd:COG0769     4 YDSRKVKPGDLFVALPGARVDGHDFIAQAIARGAVAVVTEAPGA-------LLAAGVPVIVVPDPRAALALLAAAFYGHP 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 110 S--PRVAAITGSVGKTTTRELTAAALkNRFRVHSA------IRSFNTLVGCSLT---------ILA-MAED-TEVLVLEl 170
Cdd:COG0769    77 SqkLKLIGVTGTNGKTTTTYLLAQIL-RALGKKTGligtvgNGIGGELIPSSLTtpealdlqrLLAeMVDAgVTHVVME- 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 171 gtnhfgeieemVS------------HFppEIAVITEVAPCHLEGFGSLEGVLRAKLEI-CGSPRLAAVVFNND----NCL 233
Cdd:COG0769   155 -----------VSshaldqgrvdgvRF--DVAVFTNLTRDHLDYHGTMEAYFAAKARLfDQLGPGGAAVINADdpygRRL 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 234 LKNYmsynfdNIKKIVVGSGEGASLTIKeaEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVASWLGLS 313
Cdd:COG0769   222 AAAA------PARVITYGLKADADLRAT--DIELSADG--TRFTLVTPGGEVEVRLPLIGRFNVYNALAAIAAALALGID 291

                         330
                  ....*....|....*..
gi 2527307910 314 DEEAAAGIAEISPISGR 330
Cdd:COG0769   292 LEEILAALEKLKGVPGR 308
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 20-394 4.21e-21

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 96.70  E-value: 4.21e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  20 PDIKISRGWKCDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELERLnirgeAYSGVSFIAVADTQRALA 99
Cdd:PRK11929   24 GCVAATADLRLDSREVQPGDLFVACRGAASDGRAFIDQALARGAAAVLVEAEGEDQV-----AAADALVLPVADLRKALG 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 100 SLAEEYLRRVSP--RVAAITGSVGKTTTRELTAAALK-------------NRFRVHSAIRSFNTLVGCSL-TILAMAEDT 163
Cdd:PRK11929   99 ELAARWYGRPSEqlSLVAVTGTNGKTSCAQLLAQLLTrlgkpcgsigtlgARLDGRLIPGSLTTPDAIILhRILARMRAA 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 164 EVLVLELGTNHFGEIEEMVSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEICGS-PRLAAVVFNNDNCLLKNYMSYNF 242
Cdd:PRK11929  179 GADAVAMEASSHGLEQGRLDGLRIAVAGFTNLTRDHLDYHGTMQDYEEAKAALFSKlPGLGAAVINADDPAAARLLAALP 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 243 DNIKkiVVGSGEGASLTIKEAEVRLDENGPRTSAVLCREgkDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIA 322
Cdd:PRK11929  259 RGLK--VGYSPQNAGADVQARDLRATAHGQVFTLATPDG--SYQLVTRLLGRFNVSNLLLVAAALKKLGLPLAQIARALA 334

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 323 EISPISGR--GLCKRSASRGWVIDEAYNANPASMSAAIRNTRAAAESLGVRKWAIL-------AGMR-ELGESAAEWHRR 392
Cdd:PRK11929  335 AVSPVPGRmeRVGPTAGAQGPLVVVDYAHTPDALAKALTALRPVAQARNGRLVCVFgcggdrdKGKRpEMGRIAAELADR 414


                  ..
gi 2527307910 393 II 394
Cdd:PRK11929  415 VV 416
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 30-330 5.53e-21

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 95.20  E-value: 5.53e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  30 CDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAelerlnirGEAYSGVSFIAVADTQRALASLAEEYLRRV 109
Cdd:PRK00139   20 YDSRKVKPGDLFVALPGHKVDGRDFIAQAIANGAAAVVAEAD--------GEAGTGVPVIIVPDLRKALALLAAAFYGHP 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 110 S--PRVAAITGSVGKTTTRELTAAALkNRFRVHSA------IRSFNTLVGCSLT---------ILAMAED--TEVLVLEl 170
Cdd:PRK00139   92 SdkLKLIGVTGTNGKTTTAYLLAQIL-RLLGEKTAligtlgNGIGGELIPSGLTtpdaldlqrLLAELVDagVTYAAME- 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 171 gtnhfgeieemVS------------HFppEIAVITEVAPCHLEGFGSLEGVLRAKLEICGSPRLAAVVFNNDNCllknym 238
Cdd:PRK00139  170 -----------VSshaldqgrvdglKF--DVAVFTNLSRDHLDYHGTMEDYLAAKARLFSELGLAAVINADDEV------ 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 239 SYNFDNIKKIVVGSGEGASLTIKeaEVRLDENGprTSAVLCREgkdisLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAA 318
Cdd:PRK00139  231 GRRLLALPDAYAVSMAGADLRAT--DVEYTDSG--QTFTLVTE-----VESPLIGRFNVSNLLAALAALLALGVPLEDAL 301

                         330
                  ....*....|..
gi 2527307910 319 AGIAEISPISGR 330
Cdd:PRK00139  302 AALAKLQGVPGR 313
murE TIGR01085
UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, ...
 31-394 1.59e-17

UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. An exception is Staphylococcus aureus, in which diaminopimelate is replaced by lysine in the peptidoglycan and MurE is EC 6.3.2.7. The Mycobacteria, part of the closest neighboring branch outside of the low-GC Gram-positive bacteria, use diaminopimelate. A close homolog, scoring just below the trusted cutoff, is found (with introns) in Arabidopsis thaliana. Its role is unknown. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273435 [Multi-domain]  Cd Length: 464  Bit Score: 84.68  E-value: 1.59e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  31 DSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELERLnirgeaySGVSFIAVADTQRALASLAEEYLRRVS 110
Cdd:TIGR01085  10 DSREVKPGDLFVAIKGTHVDGHDFIHDAIANGAVAVVVERDVDFYV-------APVPVIIVPDLRHALSSLAAAFYGHPS 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 111 PR--VAAITGSVGKTTTRELTAAALK-------------NRF---RVHSAIRSFNTlvGCSLTILAMAED-----TEVLV 167
Cdd:TIGR01085  83 KKlkVIGVTGTNGKTTTTSLIAQLLRllgkktgligtigYRLggnDLIKNPAALTT--PEALTLQSTLAEmveagAQYAV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 168 LELgTNH---FGEIEEMvsHFppEIAVITEVAPCHLEGFGSLEGVLRAKLEICGSPRLA--AVVFNNDncllkNYMSYNF 242
Cdd:TIGR01085 161 MEV-SSHalaQGRVRGV--RF--DAAVFTNLSRDHLDFHGTMENYFAAKASLFTELGLKrfAVINLDD-----EYGAQFV 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 243 DNIKKIVVGS-----GEGASLTIKEAEVRLDENGprTSAVLCREGKDISLSCSLFGMQHAYNMGYAFAVA-SWLGLSDEE 316
Cdd:TIGR01085 231 KRLPKDITVSaitqpADGRAQDIKITDSGYSFEG--QQFTFETPAGEGHLHTPLIGRFNVYNLLAALATLlHLGGIDLED 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 317 AAAGIAEISPISGRGLCKRSASRGWVI-DEAYnaNPASMSAAIRNTRAAAESlgvRKWAIL--AGMR------ELGESAA 387
Cdd:TIGR01085 309 IVAALEKFRGVPGRMELVDGGQKFLVIvDYAH--TPDALEKALRTLRKHKDG---RLIVVFgcGGDRdrgkrpLMGAIAE 383


                  ....*..
gi 2527307910 388 EWHRRII 394
Cdd:TIGR01085 384 QLADLVI 390
MurD COG0771
UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; ...
 107-323 2.38e-09

UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramoylalanine-D-glutamate ligase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440534 [Multi-domain]  Cd Length: 445  Bit Score: 59.32  E-value: 2.38e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 107 RRVSPRVAAITGSVGKTTTRELTAAALKN-RFRVHSA--I-RSFntlvgcsLTILAMAEDTEVLVLEL------GTnhfg 176
Cdd:COG0771   101 RLSPAPIIAITGTNGKTTTTTLIGHILKAaGLRVAVGgnIgTPL-------LDLLLEPEPPDVYVLELssfqleTT---- 169

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 177 eieemvSHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEIC-GSPRLAAVVFNNDNCLLKNYmsynFDNIKKIVVGSGEG 255
Cdd:COG0771   170 ------PSLRPDVAVILNITPDHLDRHGSMEAYAAAKARIFaNQTPDDYAVLNADDPLTRAL----AEEAKARVVPFSLK 239

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2527307910 256 ASLtikEAEVRLDENgprtsaVLCREGKDISL----SCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAE 323
Cdd:COG0771   240 EPL---EGGAGLEDG------KLVDRASGEELlpvdDLRLPGRHNLENALAALAAARALGVPPEAIREALRS 302
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 97-323 1.31e-08

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 57.00  E-value: 1.31e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  97 ALASLAEEYlrrvspRVAAITGSVGKTTTRELTAAALknrfrvHSAIRSFNTLVGcsltilamAEdtevlVLELGTN-HF 175
Cdd:COG0773    96 MLAELMRGK------RSIAVAGTHGKTTTTSMLAHIL------EEAGLDPTFLIG--------GI-----LNNFGTNaRL 150

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 176 GEIEEMVS----------HFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEICGS-PRLAAVVFNNDNCLLKNYMSYNFDN 244
Cdd:COG0773   151 GDGDYFVAeadesdgsflHYSPDIAVVTNIEADHLDIYGDLEAIKEAFHEFARNvPFYGLLVLCADDPGLRELLPRCGRP 230

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 245 IKKIvvGSGEGASLTIKEAEVrldeNGPRTSAVLCREGKDI-SLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAE 323
Cdd:COG0773   231 VITY--GFSEDADYRAENIRI----DGGGSTFDVLRRGEELgEVELNLPGRHNVLNALAAIAVALELGVDPEAIAEALAS 304
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 106-330 1.86e-08

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 56.19  E-value: 1.86e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 106 LRRVSPRVAAITGSVGKTTTRELTAAALKnrfrvHSAIRSF---NtlVGCSLTILAMAEDTEVLVLELGTNHFgeieEMV 182
Cdd:TIGR01087  97 LRLVPLPVVAITGTNGKTTTTSLLYHLLK-----AAGLKAFlggN--IGTPALEVLDQEGAELYVLELSSFQL----ETT 165

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 183 SHFPPEIAVITEVAPCHLEGFGSLEGVLRAKLEIC-GSPRLAAVVFNNDnclLKNYMSYNFDNIKKIVVGSGEgasltiK 261
Cdd:TIGR01087 166 ESLRPEIALILNISEDHLDWHGSFEDYVAAKLKIFaRQTEGDVAVLNAD---DPRFARLAQKSKAQVIWFSVE------K 236

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2527307910 262 EAEVRLdengprtsaVLCREG---KDISLSCSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPISGR 330
Cdd:TIGR01087 237 DAERGL---------CIRDGGlylKPNDLEGSLLGLHNAENILAAIALAKSLGLNLEAILEALRSFKGLPHR 299
murD PRK14106
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
 113-216 8.06e-07

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional


Pssm-ID: 184511 [Multi-domain]  Cd Length: 450  Bit Score: 51.12  E-value: 8.06e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 113 VAAITGSVGKTTTRELTAAALKNRFRvhsairsfNTLVGCSL------TILAMAEDTeVLVLELGTNHFgeieEMVSHFP 186
Cdd:PRK14106  110 IVAITGTNGKTTTTTLLGEIFKNAGR--------KTLVAGNIgyplidAVEEYGEDD-IIVAEVSSFQL----ETIKEFK 176

                          90       100       110
                  ....*....|....*....|....*....|
gi 2527307910 187 PEIAVITEVAPCHLEGFGSLEGVLRAKLEI 216
Cdd:PRK14106  177 PKVGCILNITPDHLDRHKTMENYIKAKARI 206
Mur_ligase pfam01225
Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes ...
 30-105 4.50e-06

Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460121 [Multi-domain]  Cd Length: 84  Bit Score: 44.53  E-value: 4.50e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2527307910  30 CDSREINPGDGFVALKGAATDGHLYLHQAIERGAKLLLAEAAELERLNIRGE-AYSGVSFIAVADTQRALASLAEEY 105
Cdd:pfam01225   7 IDGRGMSPGALFLALKGYRVDGSDFIESLIALGAAAVVGHDAANNISPDNPElEAAKVPGIPVIDRREALAELAAAF 83
PRK14573 PRK14573
bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;
98-377 9.40e-06

bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;


Pssm-ID: 184752 [Multi-domain]  Cd Length: 809  Bit Score: 48.27  E-value: 9.40e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  98 LASLAEEYLRRVsprvaaITGSVGKTTTRELTAAALKNRFRVHS-AIRSFNtlvgcSLTILAMAEDTEVLVLELgtnhfG 176
Cdd:PRK14573   97 LAELMQEQISIL------VSGSHGKTTVSSLITAIFQEAKKDPSyAIGGLN-----QEGLNGYSGSSEYFVAEA-----D 160

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 177 EIEEMVSHFPPEIAVITEVAPCHLEGF-GSLEGVLRAKLEICGSPRLAAVVFNNDNC-LLKNymsynfdNIKKIVVGSGE 254
Cdd:PRK14573  161 ESDGSLKHYTPEFSVITNIDNEHLSNFeGDRELLLASIQDFARKVQQINKCFYNGDCpRLKG-------CLQGHSYGFSS 233

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910 255 GASLTIkeaeVRLDENGPRTSAVLCREGK---DISLscSLFGMQHAYNMGYAFAVASWLGLSDEEAAAGIAEISPISgRG 331
Cdd:PRK14573  234 SCDLHI----LSYYQEGWRSYFSAKFLGVvyqDIEL--NLVGMHNVANAAAAMGIALTLGIDEGAIRNALKGFSGVQ-RR 306

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*.
gi 2527307910 332 LCKRSASRGWVIDEAYNANPASMSAAIRNTRAAaesLGVRKwaILA 377
Cdd:PRK14573  307 LERKNSSETFLFLEDYAHHPSEISCTLRAVRDA---VGLRR--IIA 347
Mur_ligase_C pfam02875
Mur ligase family, glutamate ligase domain; This family contains a number of related ligase ...
 341-412 3.48e-04

Mur ligase family, glutamate ligase domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460731 [Multi-domain]  Cd Length: 87  Bit Score: 39.25  E-value: 3.48e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2527307910 341 WVIDeaYNANPASMSAAIrntRAAAESLGVRKWAILAGMRELGESAAEWHRRIIGELADFdgVMLLGEEWRG 412
Cdd:pfam02875  16 VIDD--YAHNPDAMEAAL---RALRNLFPGRLILVFGGMGDRDAEFHALLGRLAAALADV--VILTGDYPRA 80
PRK14022 PRK14022
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase;
31-124 7.73e-03

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase;


Pssm-ID: 237588 [Multi-domain]  Cd Length: 481  Bit Score: 38.48  E-value: 7.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527307910  31 DSREINPGDGFVAlKGAATDgHLYLHQAIERGAKLLLAeaaelerlNIRGEAysGVSFIAVADTQRALASLAEEYLRRVS 110
Cdd:PRK14022   40 DSRTADEGTLFFA-KGAYFK-HKFLQNAITQGLKLYVS--------EKDYEV--GIPQVIVPDIKKAMSLIAMEFYDNPQ 107

                          90
                  ....*....|....*.
gi 2527307910 111 PRVA--AITGSVGKTT 124
Cdd:PRK14022  108 HKLKllAFTGTKGKTT 123
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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