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Conserved domains on  [gi|2306643424|ref|WP_260840699|]
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MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Staphylococcus]

Protein Classification

P-loop NTPase family protein( domain architecture ID 1562424)

P-loop NTPase (nucleoside triphosphate hydrolase) family protein contains two conserved sequence signatures, the Walker A motif (the P-loop proper) and Walker B motif which bind, respectively, the beta and gamma phosphate moieties of the bound nucleotide (typically ATP or GTP), and a Mg(2+) cation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HerA super family cl33869
Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase ...
 139-181 1.27e-06

Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase domains [Replication, recombination and repair];


The actual alignment was detected with superfamily member COG0433:

Pssm-ID: 440202 [Multi-domain]  Cd Length: 388  Bit Score: 49.99  E-value: 1.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2306643424 139 INLSK--TPHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDRK 181
Cdd:COG0433    40 LDLDKllNRHILILGATGSGKSNTLQVLLEELSRAGVPVLVFDPH 84
P-loop_NTPase super family cl38936
P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain ...
 128-295 8.02e-06

P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a hydrophobic residue). The Walker A and B motifs bind the beta-gamma phosphate moiety of the bound nucleotide (typically ATP or GTP) and the Mg2+ cation, respectively. The P-loop NTPases are involved in diverse cellular functions, and they can be divided into two major structural classes: the KG (kinase-GTPase) class which includes Ras-like GTPases and its circularly permutated YlqF-like; and the ASCE (additional strand catalytic E) class which includes ATPase Binding Cassette (ABC), DExD/H-like helicases, 4Fe-4S iron sulfur cluster binding proteins of NifH family, RecA-like F1-ATPases, and ATPases Associated with a wide variety of Activities (AAA). Also included are a diverse set of nucleotide/nucleoside kinase families.


The actual alignment was detected with superfamily member pfam01580:

Pssm-ID: 476819 [Multi-domain]  Cd Length: 219  Bit Score: 46.60  E-value: 8.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 128 FEIPLMKDGT----VINLSKTP-HMLISGLTGSGKSYSMYHLMYSLILKG--HEV--FVIDRKQVltKFGTVIGNDHVAD 198
Cdd:pfam01580  17 LPIALGKDISgnpeVFDLKKMPvHLLIAGATGSGKSVALNTLILSLAYMHtpEEVqlYCIDPKMG--ELSAYEDIPHLLS 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 199 EN-PNESEQIFELIERVNNIMLKRQEILKN---------DDRFKKDIEAGF--QNANWNNICLVIDELGALTQ------- 259
Cdd:pfam01580  95 VPvATDPKRALRALEWLVDEMERRYALFRAlgvrsiagyNGEIAEDPLDGFgdVFLVIYGVHVMCTAGRWLEIlpylvvi 174

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2306643424 260 -----DLAMM-KKAERDRFYSALGNIAMKGRNTGVSLMISLQ 295
Cdd:pfam01580 175 vderaELRLAaPKDSEMRVEDAIVRLAQKGRAAGIHLLLATQ 216
 
Name Accession Description Interval E-value
HerA COG0433
Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase ...
 139-181 1.27e-06

Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase domains [Replication, recombination and repair];


Pssm-ID: 440202 [Multi-domain]  Cd Length: 388  Bit Score: 49.99  E-value: 1.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2306643424 139 INLSK--TPHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDRK 181
Cdd:COG0433    40 LDLDKllNRHILILGATGSGKSNTLQVLLEELSRAGVPVLVFDPH 84
FtsK_SpoIIIE pfam01580
FtsK/SpoIIIE family; FtsK has extensive sequence similarity to wide variety of proteins from ...
 128-295 8.02e-06

FtsK/SpoIIIE family; FtsK has extensive sequence similarity to wide variety of proteins from prokaryotes and plasmids, termed the FtsK/SpoIIIE family. This domain contains a putative ATP binding P-loop motif. It is found in the FtsK cell division protein from E. coli and the stage III sporulation protein E SpoIIIE, which has roles in regulation of prespore specific gene expression in B. subtilis. A mutation in FtsK causes a temperature sensitive block in cell division and it is involved in peptidoglycan synthesis or modification. The SpoIIIE protein is implicated in intercellular chromosomal DNA transfer.


Pssm-ID: 279863 [Multi-domain]  Cd Length: 219  Bit Score: 46.60  E-value: 8.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 128 FEIPLMKDGT----VINLSKTP-HMLISGLTGSGKSYSMYHLMYSLILKG--HEV--FVIDRKQVltKFGTVIGNDHVAD 198
Cdd:pfam01580  17 LPIALGKDISgnpeVFDLKKMPvHLLIAGATGSGKSVALNTLILSLAYMHtpEEVqlYCIDPKMG--ELSAYEDIPHLLS 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 199 EN-PNESEQIFELIERVNNIMLKRQEILKN---------DDRFKKDIEAGF--QNANWNNICLVIDELGALTQ------- 259
Cdd:pfam01580  95 VPvATDPKRALRALEWLVDEMERRYALFRAlgvrsiagyNGEIAEDPLDGFgdVFLVIYGVHVMCTAGRWLEIlpylvvi 174

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2306643424 260 -----DLAMM-KKAERDRFYSALGNIAMKGRNTGVSLMISLQ 295
Cdd:pfam01580 175 vderaELRLAaPKDSEMRVEDAIVRLAQKGRAAGIHLLLATQ 216
T7_EssCb_Firm TIGR03928
type VII secretion protein EssC, C-terminal domain; This model describes the C-terminal domain, ...
 112-292 4.23e-05

type VII secretion protein EssC, C-terminal domain; This model describes the C-terminal domain, or longer subunit, of the Firmicutes type VII secretion protein EssC. This protein (homologous to EccC in Actinobacteria) and the WXG100 target proteins are the only homologous parts of type VII secretion between Firmicutes and Actinobacteria. [Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274860 [Multi-domain]  Cd Length: 1296  Bit Score: 45.75  E-value: 4.23e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424  112 FKSFEQYLQVLNSTREFEIPL-MKDGTV----INLSKTPHMLISGLTGSGKSYSMYHLMYSLILKGHE-VFVID-RKQVL 184
Cdd:TIGR03928 1059 LEEFRERYEVRKILEEGSIPIgLDEETVepvyIDLTENPHLLIVGESDDGKTNVLKSLLKTLAKQEKEkIGLIDsIDRGL 1138

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424  185 TKFGtviGNDHVADEnPNESEQIFELIERVNNIMLKRQEILKNDDRfkkdieAGFQNANWNNICLVIDELGALTQ--DLA 262
Cdd:TIGR03928 1139 LAYR---DLKEVATY-IEEKEDLKEILAELKEEIELREAAYKEALQ------NETGEPAFKPILLIIDDLEDFIQrtDLE 1208

                          170       180       190
                   ....*....|....*....|....*....|
gi 2306643424  263 MMKKaerdrfysaLGNIAMKGRNTGVSLMI 292
Cdd:TIGR03928 1209 IQDI---------LALIMKNGKKLGIHFIV 1229
TrwB_TraG_TraD_VirD4 cd01127
TrwB/TraG/TraD/VirD4 family of bacterial conjugation proteins; The TraG/TraD/VirD4 family are ...
 146-295 4.57e-04

TrwB/TraG/TraD/VirD4 family of bacterial conjugation proteins; The TraG/TraD/VirD4 family are bacterial conjugation proteins involved in type IV secretion (T4S) systems, versatile bacterial secretion systems mediating transport of protein and/or DNA. They are present in gram-negative and gram-positive bacteria, as well as archaea. They form hexameric rings and belong to the RecA-like NTPases superfamily, which also includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases.


Pssm-ID: 410871 [Multi-domain]  Cd Length: 144  Bit Score: 40.28  E-value: 4.57e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 146 HMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDrkqvlTKFGTVIGNDHVADENPNESEQIFELIERVNNIMLKRQEIl 225
Cdd:cd01127     1 NTLVLGTTGSGKTTSIVIPLLDQAARGGSVIITD-----PKGELFLVIPDRDDSFAALRALFFNQLFRALTELASLSPG- 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 226 KNDDRfkkdieagfqnanwnnICLVIDELGALTqdlammkkaerdrFYSALGNIAMKGRNTGVSLMISLQ 295
Cdd:cd01127    75 RLPRR----------------VWFILDEFANLG-------------RIPNLPNLLATGRKRGISVVLILQ 115
AAA_10 pfam12846
AAA-like domain; This family of domains contain a P-loop motif that is characteriztic of the ...
 135-181 7.05e-03

AAA-like domain; This family of domains contain a P-loop motif that is characteriztic of the AAA superfamily. Many of the proteins in this family are conjugative transfer proteins, including VirB4 components of the Type IV secretory pathway and conjugal transfer protein TrbE. This entry includes the arcaheal Vir4/HerA homolog CedB, a membrane-bound protein that is highly induced upon UV treatment and essential for DNA transfer between Sulfolobus cells.


Pssm-ID: 315512 [Multi-domain]  Cd Length: 362  Bit Score: 38.15  E-value: 7.05e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 2306643424 135 DGTVINlskTPHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDRK 181
Cdd:pfam12846  15 KGSKTN---APHSAIIGDLGGGKSVLNKTLFYYIVLLGGKALYIDPK 58
 
Name Accession Description Interval E-value
HerA COG0433
Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase ...
 139-181 1.27e-06

Archaeal DNA helicase HerA or a related bacterial ATPase, contains HAS-barrel and ATPase domains [Replication, recombination and repair];


Pssm-ID: 440202 [Multi-domain]  Cd Length: 388  Bit Score: 49.99  E-value: 1.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 2306643424 139 INLSK--TPHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDRK 181
Cdd:COG0433    40 LDLDKllNRHILILGATGSGKSNTLQVLLEELSRAGVPVLVFDPH 84
FtsK_SpoIIIE pfam01580
FtsK/SpoIIIE family; FtsK has extensive sequence similarity to wide variety of proteins from ...
 128-295 8.02e-06

FtsK/SpoIIIE family; FtsK has extensive sequence similarity to wide variety of proteins from prokaryotes and plasmids, termed the FtsK/SpoIIIE family. This domain contains a putative ATP binding P-loop motif. It is found in the FtsK cell division protein from E. coli and the stage III sporulation protein E SpoIIIE, which has roles in regulation of prespore specific gene expression in B. subtilis. A mutation in FtsK causes a temperature sensitive block in cell division and it is involved in peptidoglycan synthesis or modification. The SpoIIIE protein is implicated in intercellular chromosomal DNA transfer.


Pssm-ID: 279863 [Multi-domain]  Cd Length: 219  Bit Score: 46.60  E-value: 8.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 128 FEIPLMKDGT----VINLSKTP-HMLISGLTGSGKSYSMYHLMYSLILKG--HEV--FVIDRKQVltKFGTVIGNDHVAD 198
Cdd:pfam01580  17 LPIALGKDISgnpeVFDLKKMPvHLLIAGATGSGKSVALNTLILSLAYMHtpEEVqlYCIDPKMG--ELSAYEDIPHLLS 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 199 EN-PNESEQIFELIERVNNIMLKRQEILKN---------DDRFKKDIEAGF--QNANWNNICLVIDELGALTQ------- 259
Cdd:pfam01580  95 VPvATDPKRALRALEWLVDEMERRYALFRAlgvrsiagyNGEIAEDPLDGFgdVFLVIYGVHVMCTAGRWLEIlpylvvi 174

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2306643424 260 -----DLAMM-KKAERDRFYSALGNIAMKGRNTGVSLMISLQ 295
Cdd:pfam01580 175 vderaELRLAaPKDSEMRVEDAIVRLAQKGRAAGIHLLLATQ 216
T7_EssCb_Firm TIGR03928
type VII secretion protein EssC, C-terminal domain; This model describes the C-terminal domain, ...
 112-292 4.23e-05

type VII secretion protein EssC, C-terminal domain; This model describes the C-terminal domain, or longer subunit, of the Firmicutes type VII secretion protein EssC. This protein (homologous to EccC in Actinobacteria) and the WXG100 target proteins are the only homologous parts of type VII secretion between Firmicutes and Actinobacteria. [Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274860 [Multi-domain]  Cd Length: 1296  Bit Score: 45.75  E-value: 4.23e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424  112 FKSFEQYLQVLNSTREFEIPL-MKDGTV----INLSKTPHMLISGLTGSGKSYSMYHLMYSLILKGHE-VFVID-RKQVL 184
Cdd:TIGR03928 1059 LEEFRERYEVRKILEEGSIPIgLDEETVepvyIDLTENPHLLIVGESDDGKTNVLKSLLKTLAKQEKEkIGLIDsIDRGL 1138

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424  185 TKFGtviGNDHVADEnPNESEQIFELIERVNNIMLKRQEILKNDDRfkkdieAGFQNANWNNICLVIDELGALTQ--DLA 262
Cdd:TIGR03928 1139 LAYR---DLKEVATY-IEEKEDLKEILAELKEEIELREAAYKEALQ------NETGEPAFKPILLIIDDLEDFIQrtDLE 1208

                          170       180       190
                   ....*....|....*....|....*....|
gi 2306643424  263 MMKKaerdrfysaLGNIAMKGRNTGVSLMI 292
Cdd:TIGR03928 1209 IQDI---------LALIMKNGKKLGIHFIV 1229
FtsK COG1674
DNA segregation ATPase FtsK/SpoIIIE or related protein [Cell cycle control, cell division, ...
 130-187 5.17e-05

DNA segregation ATPase FtsK/SpoIIIE or related protein [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 441280 [Multi-domain]  Cd Length: 611  Bit Score: 45.30  E-value: 5.17e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2306643424 130 IPLMKD--GT--VINLSKTPHMLISGLTGSGKSYSMYHLMYSLILKGH--EV-FV-IDRKQV-LTKF 187
Cdd:COG1674   263 IALGKDisGEpvVADLAKMPHLLIAGATGSGKSVCINAMILSLLYKATpdEVrLIlIDPKMVeLSVY 329
VirB4 COG3451
Type IV secretory pathway, VirB4 component [Intracellular trafficking, secretion, and ...
 145-179 5.79e-05

Type IV secretory pathway, VirB4 component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442674 [Multi-domain]  Cd Length: 546  Bit Score: 44.94  E-value: 5.79e-05
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2306643424 145 PHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVID 179
Cdd:COG3451   205 GNTLILGPSGSGKSFLLKLLLLQLLRYGARIVIFD 239
TrwB_TraG_TraD_VirD4 cd01127
TrwB/TraG/TraD/VirD4 family of bacterial conjugation proteins; The TraG/TraD/VirD4 family are ...
 146-295 4.57e-04

TrwB/TraG/TraD/VirD4 family of bacterial conjugation proteins; The TraG/TraD/VirD4 family are bacterial conjugation proteins involved in type IV secretion (T4S) systems, versatile bacterial secretion systems mediating transport of protein and/or DNA. They are present in gram-negative and gram-positive bacteria, as well as archaea. They form hexameric rings and belong to the RecA-like NTPases superfamily, which also includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases.


Pssm-ID: 410871 [Multi-domain]  Cd Length: 144  Bit Score: 40.28  E-value: 4.57e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 146 HMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDrkqvlTKFGTVIGNDHVADENPNESEQIFELIERVNNIMLKRQEIl 225
Cdd:cd01127     1 NTLVLGTTGSGKTTSIVIPLLDQAARGGSVIITD-----PKGELFLVIPDRDDSFAALRALFFNQLFRALTELASLSPG- 74

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 226 KNDDRfkkdieagfqnanwnnICLVIDELGALTqdlammkkaerdrFYSALGNIAMKGRNTGVSLMISLQ 295
Cdd:cd01127    75 RLPRR----------------VWFILDEFANLG-------------RIPNLPNLLATGRKRGISVVLILQ 115
ResIII pfam04851
Type III restriction enzyme, res subunit;
148-253 3.53e-03

Type III restriction enzyme, res subunit;


Pssm-ID: 398492 [Multi-domain]  Cd Length: 162  Bit Score: 38.04  E-value: 3.53e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2306643424 148 LISGLTGSGKSYSMYHLMYSLILKGHE---VFVIDRK----QVLTKFGTVIGNDHVA---------DENPNESEQIFELI 211
Cdd:pfam04851  27 LIVMATGSGKTLTAAKLIARLFKKGPIkkvLFLVPRKdlleQALEEFKKFLPNYVEIgeiisgdkkDESVDDNKIVVTTI 106

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2306643424 212 ERVNNIMLKRQEILKNDdrfkkdieagfqnanwNNICLVIDE 253
Cdd:pfam04851 107 QSLYKALELASLELLPD----------------FFDVIIIDE 132
AAA_10 pfam12846
AAA-like domain; This family of domains contain a P-loop motif that is characteriztic of the ...
 135-181 7.05e-03

AAA-like domain; This family of domains contain a P-loop motif that is characteriztic of the AAA superfamily. Many of the proteins in this family are conjugative transfer proteins, including VirB4 components of the Type IV secretory pathway and conjugal transfer protein TrbE. This entry includes the arcaheal Vir4/HerA homolog CedB, a membrane-bound protein that is highly induced upon UV treatment and essential for DNA transfer between Sulfolobus cells.


Pssm-ID: 315512 [Multi-domain]  Cd Length: 362  Bit Score: 38.15  E-value: 7.05e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 2306643424 135 DGTVINlskTPHMLISGLTGSGKSYSMYHLMYSLILKGHEVFVIDRK 181
Cdd:pfam12846  15 KGSKTN---APHSAIIGDLGGGKSVLNKTLFYYIVLLGGKALYIDPK 58
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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