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Conserved domains on  [gi|2221000086|ref|WP_244096616|]
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LexA family transcriptional regulator [Shewanella sp. ANA-3]

Protein Classification

LexA family protein( domain architecture ID 11449429)

LexA family protein may function as a transcriptional regulator involved in the repression of one or more genes involved in the response to DNA damage (SOS response), including recA and lexA and/or may contain a S24 peptidase domain such as in the translesion error-prone DNA polymerase V autoproteolytic subunit

Gene Ontology:  GO:0003677|GO:0045892
PubMed:  10679470|10908318

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
LexA COG1974
SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, ...
 18-123 1.57e-26

SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, Signal transduction mechanisms];


:

Pssm-ID: 441577 [Multi-domain]  Cd Length: 199  Bit Score: 98.06  E-value: 1.57e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  18 ASDYSELRTNLDDLLVLHPCATWFGVAQGDSMQDEGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIF--DKKRRMLL 95
Cdd:COG1974    91 AEENIEEYLDLPEELVKNPGATFALRVKGDSMIDAGILDGDLVIVDRQLEAENGDIVVALIDGEATVKRLykEGGRVRLQ 170

                          90       100
                  ....*....|....*....|....*...
gi 2221000086  96 SANPQYHAVPIrDCDTFSVEGIVIRSVR 123
Cdd:COG1974   171 PENPAYPPIII-EGDDVEILGVVVGVIR 197
 
Name Accession Description Interval E-value
LexA COG1974
SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, ...
 18-123 1.57e-26

SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, Signal transduction mechanisms];


Pssm-ID: 441577 [Multi-domain]  Cd Length: 199  Bit Score: 98.06  E-value: 1.57e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  18 ASDYSELRTNLDDLLVLHPCATWFGVAQGDSMQDEGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIF--DKKRRMLL 95
Cdd:COG1974    91 AEENIEEYLDLPEELVKNPGATFALRVKGDSMIDAGILDGDLVIVDRQLEAENGDIVVALIDGEATVKRLykEGGRVRLQ 170

                          90       100
                  ....*....|....*....|....*...
gi 2221000086  96 SANPQYHAVPIrDCDTFSVEGIVIRSVR 123
Cdd:COG1974   171 PENPAYPPIII-EGDDVEILGVVVGVIR 197
PRK10276 PRK10276
translesion error-prone DNA polymerase V autoproteolytic subunit;
18-123 2.67e-25

translesion error-prone DNA polymerase V autoproteolytic subunit;


Pssm-ID: 182350  Cd Length: 139  Bit Score: 93.32  E-value: 2.67e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  18 ASDYSELRTNLDDLLVLHPCATWFGVAQGDSMQDEGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIFDKKRR-MLLS 96
Cdd:PRK10276   30 AADYVEQRIDLNELLIQHPSATYFVKASGDSMIDAGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTvQLIP 109

                          90       100
                  ....*....|....*....|....*..
gi 2221000086  97 ANPQYHAVPIRDCDTFSVEGIVIRSVR 123
Cdd:PRK10276  110 MNSAYSPITISSEDTLDVFGVVTHIVK 136
Peptidase_S24 pfam00717
Peptidase S24-like;
44-119 2.30e-16

Peptidase S24-like;


Pssm-ID: 425835  Cd Length: 116  Bit Score: 69.54  E-value: 2.30e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2221000086  44 AQGDSMQDeGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIF--DKKRRMLLSANPQYHAVPIRDCDTFSVEGIVI 119
Cdd:pfam00717  40 VKGDSMEP-GIPDGDLVLVDPSREARNGDIVVARLDGEATVKRLyrDGGGIRLISLNPEYPPIELPAEDDVEIIGRVV 116
S24_LexA-like cd06529
Peptidase S24 LexA-like proteins are involved in the SOS response leading to the repair of ...
 44-119 6.00e-15

Peptidase S24 LexA-like proteins are involved in the SOS response leading to the repair of single-stranded DNA within the bacterial cell. This family includes: the lambda repressor CI/C2 family and related bacterial prophage repressor proteins; LexA (EC 3.4.21.88), the repressor of genes in the cellular SOS response to DNA damage; MucA and the related UmuD proteins, which are lesion-bypass DNA polymerases, induced in response to mitogenic DNA damage; RulA, a component of the rulAB locus that confers resistance to UV, and RuvA, which is a component of the RuvABC resolvasome that catalyzes the resolution of Holliday junctions that arise during genetic recombination and DNA repair. The LexA-like proteins contain two-domains: an N-terminal DNA binding domain and a C-terminal domain (CTD) that provides LexA dimerization as well as cleavage activity. They undergo autolysis, cleaving at an Ala-Gly or a Cys-Gly bond, separating the DNA-binding domain from the rest of the protein. In the presence of single-stranded DNA, the LexA, UmuD and MucA proteins interact with RecA, activating self cleavage, thus either derepressing transcription in the case of LexA or activating the lesion-bypass polymerase in the case of UmuD and MucA. The LexA proteins are serine proteases that carry out catalysis using a serine/lysine dyad instead of the prototypical serine/histidine/aspartic acid triad found in most serine proteases. LexA sequence homologs are found in almost all of the bacterial genomes sequenced to date, covering a large number of phyla, suggesting both, an ancient origin and a widespread distribution of lexA and the SOS response.


Pssm-ID: 119397 [Multi-domain]  Cd Length: 81  Bit Score: 64.89  E-value: 6.00e-15
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2221000086  44 AQGDSMQDEgIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIFDKK---RRMLLSANPQYHAVPIRDcDTFSVEGIVI 119
Cdd:cd06529     5 VKGDSMEPT-IPDGDLVLVDPSDTPRDGDIVVARLDGELTVKRLQRRgggRLRLISDNPAYPPIEIDE-EELEIVGVVG 81
 
Name Accession Description Interval E-value
LexA COG1974
SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, ...
 18-123 1.57e-26

SOS-response transcriptional repressor LexA (RecA-mediated autopeptidase) [Transcription, Signal transduction mechanisms];


Pssm-ID: 441577 [Multi-domain]  Cd Length: 199  Bit Score: 98.06  E-value: 1.57e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  18 ASDYSELRTNLDDLLVLHPCATWFGVAQGDSMQDEGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIF--DKKRRMLL 95
Cdd:COG1974    91 AEENIEEYLDLPEELVKNPGATFALRVKGDSMIDAGILDGDLVIVDRQLEAENGDIVVALIDGEATVKRLykEGGRVRLQ 170

                          90       100
                  ....*....|....*....|....*...
gi 2221000086  96 SANPQYHAVPIrDCDTFSVEGIVIRSVR 123
Cdd:COG1974   171 PENPAYPPIII-EGDDVEILGVVVGVIR 197
PRK10276 PRK10276
translesion error-prone DNA polymerase V autoproteolytic subunit;
18-123 2.67e-25

translesion error-prone DNA polymerase V autoproteolytic subunit;


Pssm-ID: 182350  Cd Length: 139  Bit Score: 93.32  E-value: 2.67e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  18 ASDYSELRTNLDDLLVLHPCATWFGVAQGDSMQDEGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIFDKKRR-MLLS 96
Cdd:PRK10276   30 AADYVEQRIDLNELLIQHPSATYFVKASGDSMIDAGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTvQLIP 109

                          90       100
                  ....*....|....*....|....*..
gi 2221000086  97 ANPQYHAVPIRDCDTFSVEGIVIRSVR 123
Cdd:PRK10276  110 MNSAYSPITISSEDTLDVFGVVTHIVK 136
Peptidase_S24 pfam00717
Peptidase S24-like;
44-119 2.30e-16

Peptidase S24-like;


Pssm-ID: 425835  Cd Length: 116  Bit Score: 69.54  E-value: 2.30e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2221000086  44 AQGDSMQDeGIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIF--DKKRRMLLSANPQYHAVPIRDCDTFSVEGIVI 119
Cdd:pfam00717  40 VKGDSMEP-GIPDGDLVLVDPSREARNGDIVVARLDGEATVKRLyrDGGGIRLISLNPEYPPIELPAEDDVEIIGRVV 116
S24_LexA-like cd06529
Peptidase S24 LexA-like proteins are involved in the SOS response leading to the repair of ...
 44-119 6.00e-15

Peptidase S24 LexA-like proteins are involved in the SOS response leading to the repair of single-stranded DNA within the bacterial cell. This family includes: the lambda repressor CI/C2 family and related bacterial prophage repressor proteins; LexA (EC 3.4.21.88), the repressor of genes in the cellular SOS response to DNA damage; MucA and the related UmuD proteins, which are lesion-bypass DNA polymerases, induced in response to mitogenic DNA damage; RulA, a component of the rulAB locus that confers resistance to UV, and RuvA, which is a component of the RuvABC resolvasome that catalyzes the resolution of Holliday junctions that arise during genetic recombination and DNA repair. The LexA-like proteins contain two-domains: an N-terminal DNA binding domain and a C-terminal domain (CTD) that provides LexA dimerization as well as cleavage activity. They undergo autolysis, cleaving at an Ala-Gly or a Cys-Gly bond, separating the DNA-binding domain from the rest of the protein. In the presence of single-stranded DNA, the LexA, UmuD and MucA proteins interact with RecA, activating self cleavage, thus either derepressing transcription in the case of LexA or activating the lesion-bypass polymerase in the case of UmuD and MucA. The LexA proteins are serine proteases that carry out catalysis using a serine/lysine dyad instead of the prototypical serine/histidine/aspartic acid triad found in most serine proteases. LexA sequence homologs are found in almost all of the bacterial genomes sequenced to date, covering a large number of phyla, suggesting both, an ancient origin and a widespread distribution of lexA and the SOS response.


Pssm-ID: 119397 [Multi-domain]  Cd Length: 81  Bit Score: 64.89  E-value: 6.00e-15
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2221000086  44 AQGDSMQDEgIYDRDILIIDRQVNALQGAVIVAQLNGEFICKIFDKK---RRMLLSANPQYHAVPIRDcDTFSVEGIVI 119
Cdd:cd06529     5 VKGDSMEPT-IPDGDLVLVDPSDTPRDGDIVVARLDGELTVKRLQRRgggRLRLISDNPAYPPIEIDE-EELEIVGVVG 81
Peptidase_S24_S26 cd06462
The S24, S26 LexA/signal peptidase superfamily contains LexA-related and type I signal ...
 43-118 9.61e-09

The S24, S26 LexA/signal peptidase superfamily contains LexA-related and type I signal peptidase families. The S24 LexA protein domains include: the lambda repressor CI/C2 family and related bacterial prophage repressor proteins; LexA (EC 3.4.21.88), the repressor of genes in the cellular SOS response to DNA damage; MucA and the related UmuD proteins, which are lesion-bypass DNA polymerases, induced in response to mitogenic DNA damage; RulA, a component of the rulAB locus that confers resistance to UV, and RuvA, which is a component of the RuvABC resolvasome that catalyzes the resolution of Holliday junctions that arise during genetic recombination and DNA repair. The S26 type I signal peptidase (SPase) family also includes mitochondrial inner membrane protease (IMP)-like members. SPases are essential membrane-bound proteases which function to cleave away the amino-terminal signal peptide from the translocated pre-protein, thus playing a crucial role in the transport of proteins across membranes in all living organisms. All members in this superfamily are unique serine proteases that carry out catalysis using a serine/lysine dyad instead of the prototypical serine/histidine/aspartic acid triad found in most serine proteases.


Pssm-ID: 119396 [Multi-domain]  Cd Length: 84  Bit Score: 49.18  E-value: 9.61e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  43 VAQGDSMQDEgIYDRDILIIDRQVNALQ-GAVIVAQLNG-EFICKIF----DKKRRMLLSANPQYHAVPIRDCDTFSVEG 116
Cdd:cd06462     4 RVEGDSMEPT-IPDGDLVLVDKSSYEPKrGDIVVFRLPGgELTVKRViglpGEGHYFLLGDNPNSPDSRIDGPPELDIVG 82


                  ..
gi 2221000086 117 IV 118
Cdd:cd06462    83 VV 84
COG2932 COG2932
Phage repressor protein C, contains Cro/C1-type HTH and peptisase s24 domains [Mobilome: ...
 44-119 1.00e-06

Phage repressor protein C, contains Cro/C1-type HTH and peptisase s24 domains [Mobilome: prophages, transposons];


Pssm-ID: 442176  Cd Length: 121  Bit Score: 44.57  E-value: 1.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221000086  44 AQGDSMQDEgIYDRDILIIDRQVNALQ-GAVIVAQLNGEFICK--IFDKKRRMLL-SANPQYH--AVPIRDCDTFSVEGI 117
Cdd:COG2932    40 VSGDSMEPT-IRDGDIVLVDPSDTEIRdGGIYVVRTDGELLVKrlQRRPDGKLRLiSDNPAYPpiEIPPEDADEIEIIGR 118


                  ..
gi 2221000086 118 VI 119
Cdd:COG2932   119 VV 120
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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