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Conserved domains on  [gi|2162188639|ref|WP_231371460|]
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DUF1524 domain-containing protein [Bifidobacterium sp. AGR2158]

Protein Classification

DUF1524 and Excalibur domain-containing protein( domain architecture ID 10539907)

DUF1524 and Excalibur domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DUF1524 pfam07510
Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a ...
 120-257 1.04e-24

Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a conserved HXXP motif. A similar motif is seen in protein families in the His-Me finger endonuclease superfamily which suggests this family of proteins may also act as endonucleases.


:

Pssm-ID: 462188 [Multi-domain]  Cd Length: 141  Bit Score: 97.92  E-value: 1.04e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2162188639 120 DTRNDILNRDMSDKTYKANTHDCV--VATGVL-NDPYTGkrIDFTRGQDTSTAVQIDHVVALSNAWQTGAQQLSEATRTQ 196
Cdd:pfam07510   1 DPRDDEFRRDLTDKVFYRGGSRCLryILAGRLeSDLYEN--DEIDGSSKIIFDRQIEHILPQNPAWGWGAGQWDEEQREI 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2162188639 197 FANDPYNLLAVDGPANQQKSDGDaatWLPANKSF--RCEYVARQIGVKKKYELWVTQGEHDAM 257
Cdd:pfam07510  79 YVNDLGNLTLLTGSLNSAASNKD---FLPKRKAYekSCLYLTRQLAVKDKWNEEVIEARQEAL 138
Excalibur pfam05901
Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus ...
 322-357 1.08e-16

Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus subtilis and several other surface-exposed proteins from diverse bacteria are encoded in the genomes in two paralogous forms that differ by a ~45 amino acid fragment, which comprises a novel conserved domain. Sequence analysis of this domain revealed a conserved DxDxDGxxCE motif, which is strikingly similar to the Ca2+-binding loop of the calmodulin-like EF-hand domains, suggesting an evolutionary relationship between them. Functions of many of the other proteins in which the novel domain, named Excalibur (extracellular calcium-binding region), is found, as well as a structural model of its conserved motif are consistent with the notion that the Excalibur domain binds calcium. This domain is but one more example of the diversity of structural contexts surrounding the EF-hand-like calcium-binding loop in bacteria. This loop is thus more widespread than hitherto recognized and the evolution of EF-hand-like domains is probably more complex than previously appreciated.


:

Pssm-ID: 428664  Cd Length: 36  Bit Score: 72.88  E-value: 1.08e-16
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2162188639 322 YKNCAAVRAAGKAPLHRGDPGYAPKLDRDGDGIACE 357
Cdd:pfam05901   1 YKNCTEARSAGEAPIYRGAPGYRSKLDRDGDGIACE 36
 
Name Accession Description Interval E-value
DUF1524 pfam07510
Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a ...
 120-257 1.04e-24

Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a conserved HXXP motif. A similar motif is seen in protein families in the His-Me finger endonuclease superfamily which suggests this family of proteins may also act as endonucleases.


Pssm-ID: 462188 [Multi-domain]  Cd Length: 141  Bit Score: 97.92  E-value: 1.04e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2162188639 120 DTRNDILNRDMSDKTYKANTHDCV--VATGVL-NDPYTGkrIDFTRGQDTSTAVQIDHVVALSNAWQTGAQQLSEATRTQ 196
Cdd:pfam07510   1 DPRDDEFRRDLTDKVFYRGGSRCLryILAGRLeSDLYEN--DEIDGSSKIIFDRQIEHILPQNPAWGWGAGQWDEEQREI 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2162188639 197 FANDPYNLLAVDGPANQQKSDGDaatWLPANKSF--RCEYVARQIGVKKKYELWVTQGEHDAM 257
Cdd:pfam07510  79 YVNDLGNLTLLTGSLNSAASNKD---FLPKRKAYekSCLYLTRQLAVKDKWNEEVIEARQEAL 138
Excalibur pfam05901
Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus ...
 322-357 1.08e-16

Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus subtilis and several other surface-exposed proteins from diverse bacteria are encoded in the genomes in two paralogous forms that differ by a ~45 amino acid fragment, which comprises a novel conserved domain. Sequence analysis of this domain revealed a conserved DxDxDGxxCE motif, which is strikingly similar to the Ca2+-binding loop of the calmodulin-like EF-hand domains, suggesting an evolutionary relationship between them. Functions of many of the other proteins in which the novel domain, named Excalibur (extracellular calcium-binding region), is found, as well as a structural model of its conserved motif are consistent with the notion that the Excalibur domain binds calcium. This domain is but one more example of the diversity of structural contexts surrounding the EF-hand-like calcium-binding loop in bacteria. This loop is thus more widespread than hitherto recognized and the evolution of EF-hand-like domains is probably more complex than previously appreciated.


Pssm-ID: 428664  Cd Length: 36  Bit Score: 72.88  E-value: 1.08e-16
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2162188639 322 YKNCAAVRAAGKAPLHRGDPGYAPKLDRDGDGIACE 357
Cdd:pfam05901   1 YKNCTEARSAGEAPIYRGAPGYRSKLDRDGDGIACE 36
Excalibur smart00894
Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus ...
 321-357 1.01e-15

Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus subtilis and several other surface-exposed proteins from diverse bacteria are encoded in the genomes in two paralogous forms that differ by a ~45 amino acid fragment, which comprises a novel conserved domain. Sequence analysis of this domain revealed a conserved DxDxDGxxCE motif, which is strikingly similar to the Ca2+-binding loop of the calmodulin-like EF-hand domains, suggesting an evolutionary relationship between them. Functions of many of the other proteins in which the novel domain, named Excalibur (extracellular calcium-binding region), is found, as well as a structural model of its conserved motif are consistent with the notion that the Excalibur domain binds calcium. This domain is but one more example of the diversity of structural contexts surrounding the EF-hand-like calcium-binding loop in bacteria. This loop is thus more widespread than hitherto recognised and the evolution of EF-hand-like domains is probably more complex than previously appreciated.


Pssm-ID: 214891 [Multi-domain]  Cd Length: 37  Bit Score: 69.99  E-value: 1.01e-15
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 2162188639  321 YYKNCAAVRAAGKAPLHRGDPGYAPKLDRDGDGIACE 357
Cdd:smart00894   1 YYKNCKEARAAGAAPIYQGDPGYGPHLDRDGDGVACE 37
Csn1 cd09643
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short ...
 151-217 5.85e-03

CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II


Pssm-ID: 187774 [Multi-domain]  Cd Length: 799  Bit Score: 38.56  E-value: 5.85e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2162188639 151 DPYTGKRIDFTRGQDTSTaVQIDHVVALSNAWqtgaqqlseatrtqfaNDPY-NLLAVDGPANQQKSD 217
Cdd:cd09643   580 CMYTGKEIDIDDLFDLSY-YEIDHILPQSRSF----------------DDSIsNKVLVLASENQEKGD 630
cas_Csn1 TIGR01865
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile ...
 151-217 6.36e-03

CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile elements with a wide host range. This model represents a protein found only in CRISPR-containing species, near other CRISPR-associated proteins (cas), as part of the NMENI subtype of CRISPR/Cas locus. The species range so far for this protein is animal pathogens and commensals only.


Pssm-ID: 273840  Cd Length: 805  Bit Score: 38.56  E-value: 6.36e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2162188639 151 DPYTGKRIDFTRGQDTSTaVQIDHVVALSNAWqtgaqqlseatrtqfaNDPY-NLLAVDGPANQQKSD 217
Cdd:TIGR01865 584 CMYTGKEIDIDDLFDLSY-YEIDHILPQSRSF----------------DDSIsNKVLVLASENQEKGD 634
 
Name Accession Description Interval E-value
DUF1524 pfam07510
Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a ...
 120-257 1.04e-24

Protein of unknown function (DUF1524); This family of uncharacterized proteins contain a conserved HXXP motif. A similar motif is seen in protein families in the His-Me finger endonuclease superfamily which suggests this family of proteins may also act as endonucleases.


Pssm-ID: 462188 [Multi-domain]  Cd Length: 141  Bit Score: 97.92  E-value: 1.04e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2162188639 120 DTRNDILNRDMSDKTYKANTHDCV--VATGVL-NDPYTGkrIDFTRGQDTSTAVQIDHVVALSNAWQTGAQQLSEATRTQ 196
Cdd:pfam07510   1 DPRDDEFRRDLTDKVFYRGGSRCLryILAGRLeSDLYEN--DEIDGSSKIIFDRQIEHILPQNPAWGWGAGQWDEEQREI 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2162188639 197 FANDPYNLLAVDGPANQQKSDGDaatWLPANKSF--RCEYVARQIGVKKKYELWVTQGEHDAM 257
Cdd:pfam07510  79 YVNDLGNLTLLTGSLNSAASNKD---FLPKRKAYekSCLYLTRQLAVKDKWNEEVIEARQEAL 138
Excalibur pfam05901
Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus ...
 322-357 1.08e-16

Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus subtilis and several other surface-exposed proteins from diverse bacteria are encoded in the genomes in two paralogous forms that differ by a ~45 amino acid fragment, which comprises a novel conserved domain. Sequence analysis of this domain revealed a conserved DxDxDGxxCE motif, which is strikingly similar to the Ca2+-binding loop of the calmodulin-like EF-hand domains, suggesting an evolutionary relationship between them. Functions of many of the other proteins in which the novel domain, named Excalibur (extracellular calcium-binding region), is found, as well as a structural model of its conserved motif are consistent with the notion that the Excalibur domain binds calcium. This domain is but one more example of the diversity of structural contexts surrounding the EF-hand-like calcium-binding loop in bacteria. This loop is thus more widespread than hitherto recognized and the evolution of EF-hand-like domains is probably more complex than previously appreciated.


Pssm-ID: 428664  Cd Length: 36  Bit Score: 72.88  E-value: 1.08e-16
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2162188639 322 YKNCAAVRAAGKAPLHRGDPGYAPKLDRDGDGIACE 357
Cdd:pfam05901   1 YKNCTEARSAGEAPIYRGAPGYRSKLDRDGDGIACE 36
Excalibur smart00894
Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus ...
 321-357 1.01e-15

Excalibur calcium-binding domain; Extracellular Ca2+-dependent nuclease YokF from Bacillus subtilis and several other surface-exposed proteins from diverse bacteria are encoded in the genomes in two paralogous forms that differ by a ~45 amino acid fragment, which comprises a novel conserved domain. Sequence analysis of this domain revealed a conserved DxDxDGxxCE motif, which is strikingly similar to the Ca2+-binding loop of the calmodulin-like EF-hand domains, suggesting an evolutionary relationship between them. Functions of many of the other proteins in which the novel domain, named Excalibur (extracellular calcium-binding region), is found, as well as a structural model of its conserved motif are consistent with the notion that the Excalibur domain binds calcium. This domain is but one more example of the diversity of structural contexts surrounding the EF-hand-like calcium-binding loop in bacteria. This loop is thus more widespread than hitherto recognised and the evolution of EF-hand-like domains is probably more complex than previously appreciated.


Pssm-ID: 214891 [Multi-domain]  Cd Length: 37  Bit Score: 69.99  E-value: 1.01e-15
                           10        20        30
                   ....*....|....*....|....*....|....*..
gi 2162188639  321 YYKNCAAVRAAGKAPLHRGDPGYAPKLDRDGDGIACE 357
Cdd:smart00894   1 YYKNCKEARAAGAAPIYQGDPGYGPHLDRDGDGVACE 37
Csn1 cd09643
CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short ...
 151-217 5.85e-03

CRISPR/Cas system-associated protein Cas9; CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II


Pssm-ID: 187774 [Multi-domain]  Cd Length: 799  Bit Score: 38.56  E-value: 5.85e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2162188639 151 DPYTGKRIDFTRGQDTSTaVQIDHVVALSNAWqtgaqqlseatrtqfaNDPY-NLLAVDGPANQQKSD 217
Cdd:cd09643   580 CMYTGKEIDIDDLFDLSY-YEIDHILPQSRSF----------------DDSIsNKVLVLASENQEKGD 630
cas_Csn1 TIGR01865
CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile ...
 151-217 6.36e-03

CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1; CRISPR loci appear to be mobile elements with a wide host range. This model represents a protein found only in CRISPR-containing species, near other CRISPR-associated proteins (cas), as part of the NMENI subtype of CRISPR/Cas locus. The species range so far for this protein is animal pathogens and commensals only.


Pssm-ID: 273840  Cd Length: 805  Bit Score: 38.56  E-value: 6.36e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2162188639 151 DPYTGKRIDFTRGQDTSTaVQIDHVVALSNAWqtgaqqlseatrtqfaNDPY-NLLAVDGPANQQKSD 217
Cdd:TIGR01865 584 CMYTGKEIDIDDLFDLSY-YEIDHILPQSRSF----------------DDSIsNKVLVLASENQEKGD 634
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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