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Conserved domains on  [gi|2104192459|ref|WP_224595031|]
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thiaminase II [Bacillus sp. RAR_GA_16]

Protein Classification

TenA family protein( domain architecture ID 14443665)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 3-213 9.00e-126

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


:

Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 354.59  E-value: 9.00e-126
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19360     1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:cd19360    81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19360   161 DFYADDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
 
Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 3-213 9.00e-126

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 354.59  E-value: 9.00e-126
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19360     1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:cd19360    81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19360   161 DFYADDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-219 6.18e-96

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 279.08  E-value: 6.18e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   1 MSFTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLH 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  81 SEIHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKqDHPFYD 160
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPP-DHPYAE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2104192459 161 WITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQVEE 219
Cdd:COG0819   160 WIETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 11-216 3.00e-57

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 180.47  E-value: 3.00e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  11 ATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHNNLC 90
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  91 RAAGVSYEE-LQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQdHPFY-DWITFYGEK 168
Cdd:TIGR04306  81 EVLGISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHTAPE-HSVYhKWIETYSSS 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 2104192459 169 SMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:TIGR04306 160 WFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 8-216 2.01e-45

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 150.58  E-value: 2.01e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   8 REEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHN 87
Cdd:pfam03070   1 HPQLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  88 NLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWITFYGE 167
Cdd:pfam03070  81 RLAEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYAS 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 2104192459 168 KSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:pfam03070 161 EYFRAAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALD 209
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
3-217 5.06e-33

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 124.52  E-value: 5.06e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:PRK14713  320 FTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVE 399

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYeelqgyPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:PRK14713  400 SELHRSWLGDRDADT------APSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWL 473

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQV 217
Cdd:PRK14713  474 QTYADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARRR 528
 
Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 3-213 9.00e-126

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 354.59  E-value: 9.00e-126
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19360     1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:cd19360    81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19360   161 DFYADDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-219 6.18e-96

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 279.08  E-value: 6.18e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   1 MSFTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLH 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  81 SEIHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKqDHPFYD 160
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPP-DHPYAE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2104192459 161 WITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQVEE 219
Cdd:COG0819   160 WIETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 3-215 1.86e-75

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 227.05  E-value: 1.86e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19366     1 FSERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:cd19366    81 MDLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGALEHNPYREWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAY 215
Cdd:cd19366   161 EMYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 3-216 8.78e-71

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 215.13  E-value: 8.78e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19364     1 FTEELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEvnpKQDHPFY-DW 161
Cdd:cd19364    81 LALRETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADA---GSPVPLYqRW 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2104192459 162 ITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:cd19364   158 IDTYASDEFTESVQQQIDLVDRLAEEASEEEREKMKQAFLISSYYELQFWEMAYT 212
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 14-213 8.23e-58

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 181.78  E-value: 8.23e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  14 IFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQIsFVLHSEIHPHNNLCRAA 93
Cdd:cd16099     1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFLAELI-NVLDDELELHEKLLAEL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  94 GVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPkqDHPFYDWITFYGEKSMEPV 173
Cdd:cd16099    80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASLPE--HPPYRFWIDFYASDEYQEL 157

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 2104192459 174 TDQLRKILDDWAETAPErELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd16099   158 VEELLQLLDQLAAAGEE-EKEELKEIFLTSLRYELMFWDA 196
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 11-216 3.00e-57

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 180.47  E-value: 3.00e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  11 ATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHNNLC 90
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  91 RAAGVSYEE-LQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQdHPFY-DWITFYGEK 168
Cdd:TIGR04306  81 EVLGISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHTAPE-HSVYhKWIETYSSS 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 2104192459 169 SMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:TIGR04306 160 WFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 4-214 2.83e-54

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 172.70  E-value: 2.83e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   4 TEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEI 83
Cdd:cd19365     1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  84 HPHNNLCRAAGVSyeelQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPkqDHPFYDWIT 163
Cdd:cd19365    81 ELHRSFLGEFGID----AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASP--NHPYQDWID 154

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2104192459 164 FYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMA 214
Cdd:cd19365   155 TYSDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 3-213 3.16e-48

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 157.74  E-value: 3.16e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19361     1 LSERLYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFY-DW 161
Cdd:cd19361    81 MDIHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERYPAALEHEFYgEW 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2104192459 162 ITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19361   161 VKGYSSEEYAEANQELIDLLDRLTEDISEEQIEKLEEIFVNCSRYELKFWDM 212
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 13-213 4.52e-47

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 154.31  E-value: 4.52e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  13 PIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHNNLCRA 92
Cdd:cd19369     1 PIWEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  93 AGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPK-QDHPFYDWITFYGEKSME 171
Cdd:cd19369    81 FGLTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDNlESNPYKDWIEDYASEEYA 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2104192459 172 PVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19369   161 EYCKEWIDFADRLCENLSEEEKEKLKEIFRKASLYELKFWDM 202
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 8-216 2.01e-45

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 150.58  E-value: 2.01e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   8 REEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHN 87
Cdd:pfam03070   1 HPQLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  88 NLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWITFYGE 167
Cdd:pfam03070  81 RLAEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYAS 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 2104192459 168 KSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:pfam03070 161 EYFRAAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALD 209
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 20-215 3.32e-45

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 149.59  E-value: 3.32e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  20 KHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHsEIHPHNNLCRAAGVSYEE 99
Cdd:cd19367     8 NHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCAEWGISEEE 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459 100 LQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPK-QDHPFYDWITFYGEKSMEPVTDQLR 178
Cdd:cd19367    87 LEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKlEGNPYWSWIETYASDEYQEAVREGI 166

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 2104192459 179 KILDDWAETAP-ERELKRMKDYFIRSCQLEYGFWEMAY 215
Cdd:cd19367   167 ELLEKLAAERPsPARLEELVKIFATATRLEIGFWDMGL 204
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 4-215 1.27e-41

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 140.54  E-value: 1.27e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   4 TEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEI 83
Cdd:cd19363     2 TGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEF 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  84 HPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWIT 163
Cdd:cd19363    82 ANYEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIAEYHKDKLSENPNDIYRDWAS 161

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2104192459 164 FYGEKSMEPVTDQLRKILDDWAETAPERELKRmkdYFIRSCQLEYGFWEMAY 215
Cdd:cd19363   162 VYLSKEYKELVERLRRIVDKYGEGEPFEKLKR---IFKTASKYEYMFWDAAY 210
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 3-215 4.15e-38

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 131.54  E-value: 4.15e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:cd19358     1 FSDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARFLGFLANDE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 ihphNNL---CRAA-GVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLmeeVNPKQDHPF 158
Cdd:cd19358    81 ----NDYferAFAAlGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRA---AAAAPLRFK 153

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2104192459 159 Y-DWITFYGEKSMEPVTDQLRKILDDWAETAPERElkRMKDYFIRSCQLEYGFWEMAY 215
Cdd:cd19358   154 HqEWIDLHSGPEFEAWVDFLRDEVDRVGPTEEERE--RLEAVFARAVELEIAFFDAAY 209
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 16-210 6.35e-36

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 125.82  E-value: 6.35e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  16 EAIF--KHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLhSEIHPHNNLCRAA 93
Cdd:cd19368    12 EALFalYHPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRKGVL-EELKLHDSYAEEW 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  94 GVSYEElQGYPlAPTAHHYTRHMLEAAHSG--TLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWITFYGEKSME 171
Cdd:cd19368    91 GVDLPK-EVTP-DPATRKYTDFLLATASGKvkVAAYTLAAMAPCMRLYAFLGQELARALDDTEDHPYKKWIDTYSSQEFE 168

                         170       180       190
                  ....*....|....*....|....*....|....*....
gi 2104192459 172 PVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGF 210
Cdd:cd19368   169 ALALQLEDLLDKLSASLTGEELEALEKLYRRAMKLEVEF 207
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
3-217 5.06e-33

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 124.52  E-value: 5.06e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSE 82
Cdd:PRK14713  320 FTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVE 399

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  83 IHPHNNLCRAAGVSYeelqgyPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:PRK14713  400 SELHRSWLGDRDADT------APSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWL 473

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2104192459 163 TFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMAYQV 217
Cdd:PRK14713  474 QTYADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARRR 528
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 21-222 1.56e-29

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 114.67  E-value: 1.56e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  21 HPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEIHPHNNLC---RAAGVSY 97
Cdd:PTZ00347   31 LPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELKNCHHHYIdnpDAAGPEA 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  98 EELQgyplaptahhYTRHMLEAAHSGTLG--EIVAALLPCPWTYLEIGQRLMEEVNPKQDHPFYDWITFYGEKSMEPVTD 175
Cdd:PTZ00347  111 ACRK----------YVDFLLASGNADTLGpsVVIAAVIPCARLYAWVGQELTNEVELTESHPFRRWLLSYSDEPINTSVE 180

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 2104192459 176 QLRKILDDWAETAPERELKRMkdyFIRSCQLEYGFWEMAYQVEEWPV 222
Cdd:PTZ00347  181 QLESLLDKYIRPGEFSEVAQA---YRRAMELEYDFFDSFGYCLGRPV 224
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 4-212 2.31e-22

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 90.48  E-value: 2.31e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   4 TEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQISFVLHSEI 83
Cdd:cd19359     1 IDKLWNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLLAFLAAKIKSYLDYAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  84 HPHnNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPKqDHPFYD-WI 162
Cdd:cd19359    81 DFL-KTCHIKLGIPDVVDGVKPSPALKAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNEDPSDK-NTNFYKtWI 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2104192459 163 T--FYGEKSmepvTDQLRKILDDWAETAPERElKRMKDYFIRSCQLEYGFWE 212
Cdd:cd19359   159 EpnLPDPSS----AKQLEFFLNANAAWSKIDR-EKANEIFRQAMQLEINFFN 205
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 3-214 1.90e-17

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 80.79  E-value: 1.90e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   3 FTEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQIS--FVLH 80
Cdd:PRK09517  546 FTRALWEASGDIIAEINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAecIVVE 625

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  81 SEIHPhnnlcraagvsyEELQGYPLAPTAHH----YTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVNPkqDH 156
Cdd:PRK09517  626 AELHR------------SYLSGKEAPSAPSPvtmaYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLAEQNHD--EH 691

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2104192459 157 PFYDWITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEMA 214
Cdd:PRK09517  692 PYKDWLNTYSGEEFIAGTRAAIARVEKALENAGPEQRVDAARAFLSASVHEREFFDQA 749
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 5-214 1.38e-15

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 72.48  E-value: 1.38e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   5 EKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKcdtreeMTLfqEQISFVLHSEI- 83
Cdd:cd19362     2 ENLKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLRALLRASSK------APL--DKAIKILNSVFs 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  84 ------HPHNNLCRAAGVSYEELQ--GYPLAPTAhhYTRHMLeaaHSGTLG--EIVAALLPCPWTYLEIGQRLMEEVNpk 153
Cdd:cd19362    74 grdkgmEVHKFLYSELGITEDEIRrtGYNLVNYA--YTRHLY---YYSTLGwpQFLAAWAPCMWGYSEIGKYVLNSPN-- 146

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2104192459 154 qdhPFYD-WITFYGEKSMEPVTDQLRKILDDWAETaperelKRMKDYFIRSCQLEYGFWEMA 214
Cdd:cd19362   147 ---ELYKtWASFYASKDYKKRVEAILEALDSIEDT------EDIKNIFRNSVNFEIMFWDAA 199
PqqC COG5424
Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];
1-216 6.57e-14

Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];


Pssm-ID: 444176  Cd Length: 228  Bit Score: 68.38  E-value: 6.57e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   1 MSFTEKLREEATPifEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCD---TREEMT--LFQEQI 75
Cdd:COG5424     5 EEFEARLRAEIAR--RYLLKHPFLQRLREGKLTREQLRAFALQRYHYVKHFPRYLAAILSRCPdeeLRRALLenLYEEDG 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  76 SFVLHSEIHPHNNLCRAAGVSYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLPCPWTYLEIGQRLMEEVnpkQD 155
Cdd:COG5424    83 EGPEEGHIELWLRFAEALGLDREEVDARPPLPETRAAVDAYVNFCRRRSLLEAVAASLTAEGFAPEISRERLEGL---LE 159

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2104192459 156 HpfYDWITfygEKSMEPVT----------DQLRKILDDWAETAPERElkRMKDYFIRSCQLEYGFWEMAYQ 216
Cdd:COG5424   160 H--YGLPD---EEALEYFRlhaeldprhaEEALELVLRLADTPEDQE--AALEAARFKLDLLWAFLDALYR 223
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 4-213 1.36e-13

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 67.34  E-value: 1.36e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459   4 TEKLREEATPIFEAIFKHPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDtREEMTLFQEQISFVLHS-- 81
Cdd:cd19357     1 TSHLLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLLARAP-LPSSSLNQRLLDVLLGAla 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  82 ----EIHPHNNLCRAAGVSYEELQGyPLAPTAHHYTRHMLEAAHSG-TLGEIVAALlpcpWT----YLEIGQRLME--EV 150
Cdd:cd19357    80 nlrrELAFFEETAAEYGLDLPGLGV-PPSPATRAYVDFLASLASEGvSYLEGLVVL----WAtekvYLDAWSYARSflPS 154

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2104192459 151 NPKQDHPFYDWITFYGEKSMEPVTDQLRKILDDWAETAPERELKRMKDYFIRSCQLEYGFWEM 213
Cdd:cd19357   155 DADGGALYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVLERAEEVWRRVLELEEAFWPM 217
TenA_PqqC cd19370
TenA_like proteins, including PqqC and CADD; This family contains proteins with similarity to ...
 21-162 7.49e-06

TenA_like proteins, including PqqC and CADD; This family contains proteins with similarity to TenA, and includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C or PQQC proteins. PQQ is the prosthetic group of several bacterial enzymes, including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC catalyzes the last step of PQQ biogenesis which involves a ring closure and an eight-electron oxidation of the substrate [3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid (AHQQ)]. The exact molecular function of members of this family is unclear. Also belonging to this family is Chlamydia protein CADD (Chlamydia protein Associating with Death Domains), a redox protein toxin unique to Chlamydia species, which modulates host cell apoptosis; its redox activity and death domain binding ability may be required for this biological activity. CADD may have a role in folate metabolism.


Pssm-ID: 381705  Cd Length: 219  Bit Score: 45.27  E-value: 7.49e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2104192459  21 HPFVRGIAEGKLDKEQLVHYVKQDFEYLNTFVRIYGIALSKCDTREEMTLFQEQIS--FVLHSE---IHPHNNLCRAAGV 95
Cdd:cd19370    19 HPFHVAMHNGTLTKEQLQGYVANRYYYQKTIPKKLSAIMARCDDAQTRRKWLQNILdeDGGHGEpghIELWLRLGEALGL 98

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2104192459  96 SYEELQGYPLAPTAHHYTRHMLEAAHSGTLGEIVAALLpcpwTYLEIGQRLMEEVNPKQDHPFYDWI 162
Cdd:cd19370    99 TRELLSERHPLPATRFAVDTYLNFARRASWLEAAASSL----SEEFAPQRIQSRLDSWLQHYNSPWI 161
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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