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Conserved domains on  [gi|1839844709|ref|WP_169904213|]
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MULTISPECIES: GNAT family N-acetyltransferase [Pseudomonadota]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
13-142 1.65e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 85.05  E-value: 1.65e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  13 IQIRDLQPQDRAAWESLYRQY-----AEFYKEPMNEEILARTWSWLLDAQHPLqgkVVATANGVLLGLAHYRPFPePLLG 87
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAiaegtATFETEPPSEEEREAWFAAILAPGRPV---LVAEEDGEVVGFASLGPFR-PRPA 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  88 KEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALY 132
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
13-142 1.65e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 85.05  E-value: 1.65e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  13 IQIRDLQPQDRAAWESLYRQY-----AEFYKEPMNEEILARTWSWLLDAQHPLqgkVVATANGVLLGLAHYRPFPePLLG 87
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAiaegtATFETEPPSEEEREAWFAAILAPGRPV---LVAEEDGEVVGFASLGPFR-PRPA 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  88 KEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALY 132
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 26-142 3.04e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 62.54  E-value: 3.04e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  26 WESLYRQYAEFYKEPMNEEILARTWSWLLDAQHPLqgkVVATANGVLLGLAHYRPFPEPLlgkEAGFLDDLLVLPEQRGG 105
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEDWDEDASEGF---FVAEEDGELVGFASLSIIDDEP---PVGEIEGLAVAPEYRGK 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1839844709 106 GLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:pfam00583  75 GIGTALLQALLEWARERGCERIFLEVAADNLAAIALY 111
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 64-128 1.17e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.50  E-value: 1.17e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  64 VVATANGVLLGLAHYRPFPEpllGKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLR 128
Cdd:cd04301     2 LVAEDDGEIVGFASLSPDGS---GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
PRK03624 PRK03624
putative acetyltransferase; Provisional
 91-145 6.27e-03

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 34.90  E-value: 6.27e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  91 GFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLYDGL 145
Cdd:PRK03624   69 GWAYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEAL 123
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
13-142 1.65e-21

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 85.05  E-value: 1.65e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  13 IQIRDLQPQDRAAWESLYRQY-----AEFYKEPMNEEILARTWSWLLDAQHPLqgkVVATANGVLLGLAHYRPFPePLLG 87
Cdd:COG1247     2 MTIRPATPEDAPAIAAIYNEAiaegtATFETEPPSEEEREAWFAAILAPGRPV---LVAEEDGEVVGFASLGPFR-PRPA 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  88 KEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:COG1247    78 YRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALY 132
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 26-142 3.04e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 62.54  E-value: 3.04e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  26 WESLYRQYAEFYKEPMNEEILARTWSWLLDAQHPLqgkVVATANGVLLGLAHYRPFPEPLlgkEAGFLDDLLVLPEQRGG 105
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEDWDEDASEGF---FVAEEDGELVGFASLSIIDDEP---PVGEIEGLAVAPEYRGK 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1839844709 106 GLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:pfam00583  75 GIGTALLQALLEWARERGCERIFLEVAADNLAAIALY 111
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-142 4.34e-13

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 62.38  E-value: 4.34e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  39 EPMNEEiLARTWSWLLDAQHplqgkVVATANGVLLGLAHYRPfpeplLGKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQA 118
Cdd:COG0454    18 EALDAE-LKAMEGSLAGAEF-----IAVDDKGEPIGFAGLRR-----LDDKVLELKRLYVLPEYRGKGIGKALLEALLEW 86

                          90       100
                  ....*....|....*....|....
gi 1839844709 119 GKEQGWPFLRWITAQDNAQARRLY 142
Cdd:COG0454    87 ARERGCTALELDTLDGNPAAIRFY 110
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 87-145 1.96e-12

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 59.67  E-value: 1.96e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1839844709  87 GKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLYDGL 145
Cdd:COG0456    10 GGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKL 68
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
15-142 3.21e-12

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 60.48  E-value: 3.21e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  15 IRDLQPQDRAAWESLYRQYaefYKEPMNEEILARtwswlLDAQHPLQGKVVATANGVLLGLAHYRPFPEPLlGKEAGFLD 94
Cdd:COG3153     1 IRPATPEDAEAIAALLRAA---FGPGREAELVDR-----LREDPAAGLSLVAEDDGEIVGHVALSPVDIDG-EGPALLLG 71

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1839844709  95 DLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITaqdNAQARRLY 142
Cdd:COG3153    72 PLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG---DPSLLPFY 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 13-142 2.06e-11

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 58.08  E-value: 2.06e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  13 IQIRDLQPQDRAAWESLYRQYAefykepmneeilartwswlldAQHPLQGKVVATANGVLLGLAHYRPFPEpllgkEAGF 92
Cdd:COG1246     1 MTIRPATPDDVPAILELIRPYA---------------------LEEEIGEFWVAEEDGEIVGCAALHPLDE-----DLAE 54

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1839844709  93 LDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITaqdNAQARRLY 142
Cdd:COG1246    55 LRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLT---TSAAIHFY 101
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
91-142 5.00e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 47.98  E-value: 5.00e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1839844709  91 GFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLY 142
Cdd:COG3393    16 AEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLY 67
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 64-128 1.17e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.50  E-value: 1.17e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  64 VVATANGVLLGLAHYRPFPEpllGKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLR 128
Cdd:cd04301     2 LVAEDDGEIVGFASLSPDGS---GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 64-142 1.45e-07

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 46.68  E-value: 1.45e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1839844709  64 VVATANGVLLGLAHYRPFPEpllgKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGwpfLRWITAQDNAQARRLY 142
Cdd:pfam13508   6 FVAEDDGKIVGFAALLPLDD----EGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGG---IKLLELETTNRAAAFY 77
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 11-142 1.79e-06

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 45.38  E-value: 1.79e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  11 DGIQIRDLQPQDRAAWESLYRQYAEFYKEPMNEEILARTWSWLLDAQHPLQGK------VVATANGVLLGLAHYRPFPEP 84
Cdd:COG1670     6 ERLRLRPLRPEDAEALAELLNDPEVARYLPGPPYSLEEARAWLERLLADWADGgalpfaIEDKEDGELIGVVGLYDIDRA 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1839844709  85 LLGKEAGFLddllVLPEQRGGGLGRRLIEAVAQAGKEQ-GWPFLRWITAQDNAQARRLY 142
Cdd:COG1670    86 NRSAEIGYW----LAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPDNTASIRVL 140
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
55-142 2.00e-06

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 44.79  E-value: 2.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  55 DAQHplqgkVVATANGVLLGLAHYRPfpeplLGKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLrWITAQd 134
Cdd:COG2153    33 DARH-----LLAYDDGELVATARLLP-----PGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRI-VLSAQ- 100


                  ....*...
gi 1839844709 135 nAQARRLY 142
Cdd:COG2153   101 -AHAVGFY 107
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 85-154 3.27e-05

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 40.39  E-value: 3.27e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  85 LLGKEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAqARRLYDGLAQATAWVTY 154
Cdd:pfam08445  16 CLRLPGGELGALQTLPEHRRRGLGSRLVAALARGIAERGITPFAVVVAGNTP-SRRLYEKLGFRKIDETY 84
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 64-142 2.07e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 36.09  E-value: 2.07e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1839844709  64 VVATANGVLLGLAHYRpfpepllgkEAGFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGwPFLRWITAqdNAQ--ARRL 141
Cdd:pfam13673  34 FVAFEGGQIVGVIALR---------DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDG-IKLSELTV--NASpyAVPF 101


                  .
gi 1839844709 142 Y 142
Cdd:pfam13673 102 Y 102
PRK03624 PRK03624
putative acetyltransferase; Provisional
 91-145 6.27e-03

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 34.90  E-value: 6.27e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1839844709  91 GFLDDLLVLPEQRGGGLGRRLIEAVAQAGKEQGWPFLRWITAQDNAQARRLYDGL 145
Cdd:PRK03624   69 GWAYYLAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEAL 123
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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