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Conserved domains on  [gi|1762107327|ref|WP_151677778|]
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transcription termination/antitermination NusG family protein [Brucella tritici]

Protein Classification

transcription termination/antitermination NusG family protein( domain architecture ID 10492263)

transcription termination/antitermination NusG (N-Utilization Substance G) family protein may be involved in transcription elongation and termination in bacteria

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
NusG pfam02357
Transcription termination factor nusG;
52-147 4.64e-19

Transcription termination factor nusG;


:

Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 78.42  E-value: 4.64e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  52 WLVLQVVSGRELSVRDALEKENIEVLVPIKLGPAVKRQHRVIppKKQPVINGYVLARCAVTNQCMAGLLSFDDVIAILGG 131
Cdd:pfam02357   3 WYVLQTYSGKEKKVKENLERQGIEVFLPTEEVVEIRNGKKKV--VERPLFPGYVFVRMDMTDETWHLVRSTPGVTGFVGG 80
                          90
                  ....*....|....*.
gi 1762107327 132 YEKPFLVSDEKISVFK 147
Cdd:pfam02357  81 SGKPTPIPDEEVERIL 96
 
Name Accession Description Interval E-value
NusG pfam02357
Transcription termination factor nusG;
52-147 4.64e-19

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 78.42  E-value: 4.64e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  52 WLVLQVVSGRELSVRDALEKENIEVLVPIKLGPAVKRQHRVIppKKQPVINGYVLARCAVTNQCMAGLLSFDDVIAILGG 131
Cdd:pfam02357   3 WYVLQTYSGKEKKVKENLERQGIEVFLPTEEVVEIRNGKKKV--VERPLFPGYVFVRMDMTDETWHLVRSTPGVTGFVGG 80
                          90
                  ....*....|....*.
gi 1762107327 132 YEKPFLVSDEKISVFK 147
Cdd:pfam02357  81 SGKPTPIPDEEVERIL 96
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
47-218 3.45e-18

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 78.33  E-value: 3.45e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  47 SDDSPWLVLQVVSGRELSVRDALEK--ENIEVLVPIKLGPAVKRQHRVIppKKQPVINGYVLARCAVTNQCMAGLLSFDD 124
Cdd:COG0250     2 SMEKRWYVVHTYSGYEKKVKENLERriEGIEVFVPTEEVVEIKNGKKKT--VERPLFPGYVFVRMDLTDESWYLVRNTPG 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327 125 VIAILGGYEKPFLVSDEKISVFKSKAEDGKyDYEHFHRKFNGVRWVRMADGPFAGSRAELVAgGRKGNGLAVIEAMVLGR 204
Cdd:COG0250    80 VTGFVGFGGKPAPLPDEEVERILARLEEGE-EKPRPKVDFEVGDRVRITDGPFAGFEGTVEE-VDPEKGRVKVLVSIFGR 157
                         170
                  ....*....|....
gi 1762107327 205 PVAMTVPIAILEPL 218
Cdd:COG0250   158 ETPVELDFSQVEKI 171
NGN cd08000
N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization ...
51-147 3.85e-18

N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization Substance G (NusG) and its eukaryotic homolog Spt5 are involved in transcription elongation and termination. NusG contains an NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein composed of an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms a Spt4-Spt5 complex that is an essential RNA Polymerase II elongation factor. NusG was originally discovered as an N-dependent antitermination enhancing activity in Escherichia coli and has a variety of functions, such as being involved in RNA polymerase elongation and Rho-termination in bacteria. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The diverse activities suggest that, after diverging from a common ancestor, NusG proteins became specialized in different bacteria.


Pssm-ID: 193574 [Multi-domain]  Cd Length: 99  Bit Score: 76.20  E-value: 3.85e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  51 PWLVLQVVSGRELSVRDALEKEN----IEVLVPIKLGPavKRQHRVIPPKKQPVINGYVLARCAVTNQCMAGLLSFDDVI 126
Cdd:cd08000     1 NWYVLFVKTGREEKVEKLLEKRFeandIEAFVPKKEVP--ERKRGKIEEVIKPLFPGYVFVETDLSPELYELIREVPGVI 78
                          90       100
                  ....*....|....*....|.
gi 1762107327 127 AILGGYEKPFLVSDEKISVFK 147
Cdd:cd08000    79 GILGNGEEPSPVSDEEIEMIL 99
 
Name Accession Description Interval E-value
NusG pfam02357
Transcription termination factor nusG;
52-147 4.64e-19

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 78.42  E-value: 4.64e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  52 WLVLQVVSGRELSVRDALEKENIEVLVPIKLGPAVKRQHRVIppKKQPVINGYVLARCAVTNQCMAGLLSFDDVIAILGG 131
Cdd:pfam02357   3 WYVLQTYSGKEKKVKENLERQGIEVFLPTEEVVEIRNGKKKV--VERPLFPGYVFVRMDMTDETWHLVRSTPGVTGFVGG 80
                          90
                  ....*....|....*.
gi 1762107327 132 YEKPFLVSDEKISVFK 147
Cdd:pfam02357  81 SGKPTPIPDEEVERIL 96
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
47-218 3.45e-18

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 78.33  E-value: 3.45e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  47 SDDSPWLVLQVVSGRELSVRDALEK--ENIEVLVPIKLGPAVKRQHRVIppKKQPVINGYVLARCAVTNQCMAGLLSFDD 124
Cdd:COG0250     2 SMEKRWYVVHTYSGYEKKVKENLERriEGIEVFVPTEEVVEIKNGKKKT--VERPLFPGYVFVRMDLTDESWYLVRNTPG 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327 125 VIAILGGYEKPFLVSDEKISVFKSKAEDGKyDYEHFHRKFNGVRWVRMADGPFAGSRAELVAgGRKGNGLAVIEAMVLGR 204
Cdd:COG0250    80 VTGFVGFGGKPAPLPDEEVERILARLEEGE-EKPRPKVDFEVGDRVRITDGPFAGFEGTVEE-VDPEKGRVKVLVSIFGR 157
                         170
                  ....*....|....
gi 1762107327 205 PVAMTVPIAILEPL 218
Cdd:COG0250   158 ETPVELDFSQVEKI 171
NGN cd08000
N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization ...
51-147 3.85e-18

N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization Substance G (NusG) and its eukaryotic homolog Spt5 are involved in transcription elongation and termination. NusG contains an NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein composed of an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms a Spt4-Spt5 complex that is an essential RNA Polymerase II elongation factor. NusG was originally discovered as an N-dependent antitermination enhancing activity in Escherichia coli and has a variety of functions, such as being involved in RNA polymerase elongation and Rho-termination in bacteria. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The diverse activities suggest that, after diverging from a common ancestor, NusG proteins became specialized in different bacteria.


Pssm-ID: 193574 [Multi-domain]  Cd Length: 99  Bit Score: 76.20  E-value: 3.85e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  51 PWLVLQVVSGRELSVRDALEKEN----IEVLVPIKLGPavKRQHRVIPPKKQPVINGYVLARCAVTNQCMAGLLSFDDVI 126
Cdd:cd08000     1 NWYVLFVKTGREEKVEKLLEKRFeandIEAFVPKKEVP--ERKRGKIEEVIKPLFPGYVFVETDLSPELYELIREVPGVI 78
                          90       100
                  ....*....|....*....|.
gi 1762107327 127 AILGGYEKPFLVSDEKISVFK 147
Cdd:cd08000    79 GILGNGEEPSPVSDEEIEMIL 99
NGN_SP_UpxY cd09895
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; ...
51-109 6.62e-05

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; The N-Utilization Substance G (NusG) proteins are involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS (lipopolysaccharide) biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. UpxY proteins, UpxY proteins, where the x is replaced by the letter designation of the specific polysaccharide (UpaY to UphY), are a family of NusG SP factors that act specifically in transcriptional antitermination of operons from which they are encoded. UpxYs are necessary and specific for transcription regulation of the polysaccharide biosynthesis operon. Orthologs of the NusG gene exist in all bacteria, but their functions and requirements are different. The NusG N-terminal (NGN) domain is similar in all NusG orthologs, but its C-terminal domain and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193584 [Multi-domain]  Cd Length: 95  Bit Score: 40.64  E-value: 6.62e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1762107327  51 PWLVLQVVSGRELSVRDALEKENIEVLVPIKLGPaVKRQHRVIpPKKQPVINGYVLARC 109
Cdd:cd09895     1 PWYALYTFPRREKKVAEYLEKKGIECFLPMQYEV-RQWSGRKK-RVEVPLFPNLVFVHI 57
NGN_SP cd09886
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The ...
52-147 3.89e-04

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggest that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193575 [Multi-domain]  Cd Length: 97  Bit Score: 38.50  E-value: 3.89e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1762107327  52 WLVLQVVSGRELSVRDALEKENIEVLVPiKLGPAVKRQHRVIpPKKQPVINGYVLARCAVTNQCMAG-LLSFDDVIAILG 130
Cdd:cd09886     2 WYALRTNPGCEQRAEEALEARGVEAFLP-MLTEERKRRRKKF-DVERPLFPGYVFARLDRSKGQDTStIRACDGVLGVVG 79
                          90
                  ....*....|....*..
gi 1762107327 131 GYEKPFLVSDEKISVFK 147
Cdd:cd09886    80 FDGRPAPVPEQEMRDLR 96
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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