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Conserved domains on  [gi|1750427576|ref|WP_150019651|]
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MULTISPECIES: insecticidal delta-endotoxin Cry8Ea1 family protein [Pantoea]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Endotoxin_N super family cl04339
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 73-261 2.60e-05

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


The actual alignment was detected with superfamily member pfam03945:

Pssm-ID: 397850  Cd Length: 218  Bit Score: 45.21  E-value: 2.60e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576  73 FVPELGGLINAVLNLFW-------PSGEDTFTKIKAQIEALVNEKIDQSTWSNLQAIISELHTKMSYFKQYidKQNF--- 142
Cdd:pfam03945   3 LIPFVGPVLSFIADLIWthlietdKSTPELFNSYRQMIQDLINNSLTQYDDNKLKALLQGCSLSLKDFRTH--VKNLkad 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576 143 -------EMARDIYTALSPwllGIDERFKVDGMSKKY--YFVPLYVMVVDIIYSFRIECIVHAVTIGLTDNEVsserKYI 213
Cdd:pfam03945  81 pknatlkSTVNTIYLNLEN---DIPQKYLKDCRKEGYeaYELPLFVLMATMHLGLLKEGISNGKDWGISDSDV----KIF 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1750427576 214 SDLLSDNNSGAINY-LSAQKDNLAADSKQQVSQCSYYGQYNQICSTFNY 261
Cdd:pfam03945 154 IDKFNKWIVKYAEYcYTAYVKGLAKIQKESANIKDPVKQWNDLNDFRNF 202
 
Name Accession Description Interval E-value
Endotoxin_N pfam03945
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 73-261 2.60e-05

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397850  Cd Length: 218  Bit Score: 45.21  E-value: 2.60e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576  73 FVPELGGLINAVLNLFW-------PSGEDTFTKIKAQIEALVNEKIDQSTWSNLQAIISELHTKMSYFKQYidKQNF--- 142
Cdd:pfam03945   3 LIPFVGPVLSFIADLIWthlietdKSTPELFNSYRQMIQDLINNSLTQYDDNKLKALLQGCSLSLKDFRTH--VKNLkad 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576 143 -------EMARDIYTALSPwllGIDERFKVDGMSKKY--YFVPLYVMVVDIIYSFRIECIVHAVTIGLTDNEVsserKYI 213
Cdd:pfam03945  81 pknatlkSTVNTIYLNLEN---DIPQKYLKDCRKEGYeaYELPLFVLMATMHLGLLKEGISNGKDWGISDSDV----KIF 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1750427576 214 SDLLSDNNSGAINY-LSAQKDNLAADSKQQVSQCSYYGQYNQICSTFNY 261
Cdd:pfam03945 154 IDKFNKWIVKYAEYcYTAYVKGLAKIQKESANIKDPVKQWNDLNDFRNF 202
 
Name Accession Description Interval E-value
Endotoxin_N pfam03945
delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by ...
 73-261 2.60e-05

delta endotoxin, N-terminal domain; This family contains insecticidal toxins produced by Bacillus species of bacteria. During spore formation the bacteria produce crystals of this protein. When an insect ingests these proteins they are activated by proteolytic cleavage. The N terminus is cleaved in all of the proteins and a C terminal extension is cleaved in some members. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. This activated region of the delta endotoxin is composed of three structural domains. The N-terminal helical domain is involved in membrane insertion and pore formation. The second and third domains are involved in receptor binding.


Pssm-ID: 397850  Cd Length: 218  Bit Score: 45.21  E-value: 2.60e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576  73 FVPELGGLINAVLNLFW-------PSGEDTFTKIKAQIEALVNEKIDQSTWSNLQAIISELHTKMSYFKQYidKQNF--- 142
Cdd:pfam03945   3 LIPFVGPVLSFIADLIWthlietdKSTPELFNSYRQMIQDLINNSLTQYDDNKLKALLQGCSLSLKDFRTH--VKNLkad 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1750427576 143 -------EMARDIYTALSPwllGIDERFKVDGMSKKY--YFVPLYVMVVDIIYSFRIECIVHAVTIGLTDNEVsserKYI 213
Cdd:pfam03945  81 pknatlkSTVNTIYLNLEN---DIPQKYLKDCRKEGYeaYELPLFVLMATMHLGLLKEGISNGKDWGISDSDV----KIF 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1750427576 214 SDLLSDNNSGAINY-LSAQKDNLAADSKQQVSQCSYYGQYNQICSTFNY 261
Cdd:pfam03945 154 IDKFNKWIVKYAEYcYTAYVKGLAKIQKESANIKDPVKQWNDLNDFRNF 202
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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