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Conserved domains on  [gi|1710302846|ref|WP_143366368|]
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N-6 DNA methylase, partial [Escherichia coli]

Protein Classification

N-6 DNA methylase family protein( domain architecture ID 1006218)

N-6 DNA methylase family protein might be a component of a type I or type IC restriction system and protect the host DNA from digestion via sequence-specific methylation

CATH:  1.20.1260.30
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:0003677
PubMed:  35205080
SCOP:  4003146

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HsdM super family cl33828
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
1-79 2.51e-25

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


The actual alignment was detected with superfamily member COG0286:

Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 93.71  E-value: 2.51e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1710302846   1 SAGMFVQSVKFVESHQGK-SRDIALYGQELTATTYKLAKMNLAIRGLS-ANLgeRPADTFFSDQHPDLKADYILANPPFN 78
Cdd:COG0286    54 SGGFLVEAAEYLKEHGGDeRKKLSLYGQEINPTTYRLAKMNLLLHGIGdPNI--ELGDTLSNDGDELEKFDVVLANPPFG 131


                  .
gi 1710302846  79 L 79
Cdd:COG0286   132 G 132
 
Name Accession Description Interval E-value
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
1-79 2.51e-25

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 93.71  E-value: 2.51e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1710302846   1 SAGMFVQSVKFVESHQGK-SRDIALYGQELTATTYKLAKMNLAIRGLS-ANLgeRPADTFFSDQHPDLKADYILANPPFN 78
Cdd:COG0286    54 SGGFLVEAAEYLKEHGGDeRKKLSLYGQEINPTTYRLAKMNLLLHGIGdPNI--ELGDTLSNDGDELEKFDVVLANPPFG 131


                  .
gi 1710302846  79 L 79
Cdd:COG0286   132 G 132
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 1-81 1.10e-24

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 93.15  E-value: 1.10e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1710302846   1 SAGMFVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLS-ANLGERPADTFFSDQHPDL-KADYILANPPFN 78
Cdd:pfam02384  56 SGGFLIQAEKFVKEHDGDTNDLSIYGQEKNPTTYRLARMNMILHGIEyDDFHIRHGDTLTSPKFEDDkKFDVVVANPPFS 135


                  ...
gi 1710302846  79 LAG 81
Cdd:pfam02384 136 DKW 138
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 18-80 2.15e-05

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 40.71  E-value: 2.15e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1710302846  18 KSRDIALYGQELTATTYKLAKMNLAIRGLS-ANLGERPADTFFSDQHPDLKA-DYILANPPFNLA 80
Cdd:TIGR00497 242 KTSLVSYYGQEINHTTYNLCRMNMILHNIDyANFNIINADTLTTKEWENENGfEVVVSNPPYSIS 306
 
Name Accession Description Interval E-value
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
1-79 2.51e-25

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 93.71  E-value: 2.51e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1710302846   1 SAGMFVQSVKFVESHQGK-SRDIALYGQELTATTYKLAKMNLAIRGLS-ANLgeRPADTFFSDQHPDLKADYILANPPFN 78
Cdd:COG0286    54 SGGFLVEAAEYLKEHGGDeRKKLSLYGQEINPTTYRLAKMNLLLHGIGdPNI--ELGDTLSNDGDELEKFDVVLANPPFG 131


                  .
gi 1710302846  79 L 79
Cdd:COG0286   132 G 132
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 1-81 1.10e-24

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 93.15  E-value: 1.10e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1710302846   1 SAGMFVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLS-ANLGERPADTFFSDQHPDL-KADYILANPPFN 78
Cdd:pfam02384  56 SGGFLIQAEKFVKEHDGDTNDLSIYGQEKNPTTYRLARMNMILHGIEyDDFHIRHGDTLTSPKFEDDkKFDVVVANPPFS 135


                  ...
gi 1710302846  79 LAG 81
Cdd:pfam02384 136 DKW 138
hsdM TIGR00497
type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine ...
 18-80 2.15e-05

type I restriction system adenine methylase (hsdM); Function: methylation of specific adenine residues; required for both restriction and modification activities. The ECOR124/3 I enzyme recognizes 5'GAA(N7)RTCG. for E.coli see (J. Mol. Biol. 257: 960-969 (1996)). [DNA metabolism, Restriction/modification]


Pssm-ID: 211578 [Multi-domain]  Cd Length: 501  Bit Score: 40.71  E-value: 2.15e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1710302846  18 KSRDIALYGQELTATTYKLAKMNLAIRGLS-ANLGERPADTFFSDQHPDLKA-DYILANPPFNLA 80
Cdd:TIGR00497 242 KTSLVSYYGQEINHTTYNLCRMNMILHNIDyANFNIINADTLTTKEWENENGfEVVVSNPPYSIS 306
TrmN6 COG4123
tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) ...
 18-81 6.99e-03

tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) A37 N6-methylase TrmN6 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 443299 [Multi-domain]  Cd Length: 238  Bit Score: 33.19  E-value: 6.99e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1710302846  18 KSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPAD-TFFSDQHPDLKADYILANPPFNLAG 81
Cdd:COG4123    58 RSPGARITGVEIQPEAAELARRNVALNGLEDRITVIHGDlKEFAAELPPGSFDLVVSNPPYFKAG 122
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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