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Conserved domains on  [gi|1473338160|ref|WP_117805338|]
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TlpA disulfide reductase family protein [Blautia sp. AF32-4BH]

Protein Classification

TlpA disulfide reductase family protein( domain architecture ID 10121831)

TlpA disulfide reductase family protein such as Bradyrhizobium japonicum thiol:disulfide interchange protein TlpA, an unusual thioredoxin which has been implicated in the biogenesis of cytochrome aa3 and also characterized as a reductant for the copper metallochaperone ScoI

CATH:  3.40.30.10
Gene Ontology:  GO:0015036
PubMed:  9099998|10049365|15558583
SCOP:  3000031

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TlpA_like_family cd02966
TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are ...
 168-287 4.00e-25

TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are bacterial protein disulfide reductases with important roles in cytochrome maturation. They are membrane-anchored proteins with a soluble TRX domain containing a CXXC motif located in the periplasm. The TRX domains of this family contain an insert, approximately 25 residues in length, which correspond to an extra alpha helix and a beta strand when compared with TRX. TlpA catalyzes an essential reaction in the biogenesis of cytochrome aa3, while ResA and DsbE are essential proteins in cytochrome c maturation. Also included in this family are proteins containing a TlpA-like TRX domain with domain architectures similar to E. coli DipZ protein, and the N-terminal TRX domain of PilB protein from Neisseria which acts as a disulfide reductase that can recylce methionine sulfoxide reductases.


:

Pssm-ID: 239264 [Multi-domain]  Cd Length: 116  Bit Score: 97.31  E-value: 4.00e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDtlDNNEAGIKEAkeiLKAQ 247
Cdd:cd02966     2 FSLPDLDGKPVSLSDL-KGKVVLVNFWASWCPPCRAEMPELEALAKEYKDDGVEVVGVNVD--DDDPAAVKAF---LKKY 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1473338160 248 GASYKNLtFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:cd02966    76 GITFPVL-LDPDGELAKAYG---VRGLPTTFLIDRDGRIR 111
 
Name Accession Description Interval E-value
TlpA_like_family cd02966
TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are ...
 168-287 4.00e-25

TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are bacterial protein disulfide reductases with important roles in cytochrome maturation. They are membrane-anchored proteins with a soluble TRX domain containing a CXXC motif located in the periplasm. The TRX domains of this family contain an insert, approximately 25 residues in length, which correspond to an extra alpha helix and a beta strand when compared with TRX. TlpA catalyzes an essential reaction in the biogenesis of cytochrome aa3, while ResA and DsbE are essential proteins in cytochrome c maturation. Also included in this family are proteins containing a TlpA-like TRX domain with domain architectures similar to E. coli DipZ protein, and the N-terminal TRX domain of PilB protein from Neisseria which acts as a disulfide reductase that can recylce methionine sulfoxide reductases.


Pssm-ID: 239264 [Multi-domain]  Cd Length: 116  Bit Score: 97.31  E-value: 4.00e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDtlDNNEAGIKEAkeiLKAQ 247
Cdd:cd02966     2 FSLPDLDGKPVSLSDL-KGKVVLVNFWASWCPPCRAEMPELEALAKEYKDDGVEVVGVNVD--DDDPAAVKAF---LKKY 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1473338160 248 GASYKNLtFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:cd02966    76 GITFPVL-LDPDGELAKAYG---VRGLPTTFLIDRDGRIR 111
TrxA COG0526
Thiol-disulfide isomerase or thioredoxin [Posttranslational modification, protein turnover, ...
 168-314 1.60e-24

Thiol-disulfide isomerase or thioredoxin [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440292 [Multi-domain]  Cd Length: 139  Bit Score: 96.30  E-value: 1.60e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMggEVVGINMDtlDNNEAgikeAKEILKAQ 247
Cdd:COG0526    11 FTLTDLDGKPLSLADL-KGKPVLVNFWATWCPPCRAEMPVLKELAEEYGGV--VFVGVDVD--ENPEA----VKAFLKEL 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1473338160 248 GASYKNLtFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIVGEpFMGGIDdqsnYDQLMKQIQSVLDQ 314
Cdd:COG0526    82 GLPYPVL-LDPDGELAKAYG---VRGIPTTVLIDKDGKIVAR-HVGPLS----PEELEEALEKLLAK 139
AhpC-TSA pfam00578
AhpC/TSA family; This family contains proteins related to alkyl hydroperoxide reductase (AhpC) ...
 168-287 8.11e-14

AhpC/TSA family; This family contains proteins related to alkyl hydroperoxide reductase (AhpC) and thiol specific antioxidant (TSA).


Pssm-ID: 425763 [Multi-domain]  Cd Length: 124  Bit Score: 66.86  E-value: 8.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVddSLFA-KNKVTVVNFW-FSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDTLDNneagikeAKEILK 245
Cdd:pfam00578   8 FELPDGDGGTV--SLSDyRGKWVVLFFYpADWTPVCTTELPALADLYEEFKKLGVEVLGVSVDSPES-------HKAFAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1473338160 246 AQGASYkNLTFDSNSTV-GNY-AGKGNIM-AFPTTVLVDKDGNIV 287
Cdd:pfam00578  79 KYGLPF-PLLSDPDGEVaRAYgVLNEEEGgALRATFVIDPDGKVR 122
PRK03147 PRK03147
thiol-disulfide oxidoreductase ResA;
168-287 4.20e-10

thiol-disulfide oxidoreductase ResA;


Pssm-ID: 179545 [Multi-domain]  Cd Length: 173  Bit Score: 57.71  E-value: 4.20e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDtldnneagikEAKEILKAQ 247
Cdd:PRK03147   44 FVLTDLEGKKIELKDL-KGKGVFLNFWGTWCKPCEKEMPYMNELYPKYKEKGVEIIAVNVD----------ETELAVKNF 112

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1473338160 248 GASYkNLTF----DSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:PRK03147  113 VNRY-GLTFpvaiDKGRQVIDAYG---VGPLPTTFLIDKDGKVV 152
 
Name Accession Description Interval E-value
TlpA_like_family cd02966
TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are ...
 168-287 4.00e-25

TlpA-like family; composed of TlpA, ResA, DsbE and similar proteins. TlpA, ResA and DsbE are bacterial protein disulfide reductases with important roles in cytochrome maturation. They are membrane-anchored proteins with a soluble TRX domain containing a CXXC motif located in the periplasm. The TRX domains of this family contain an insert, approximately 25 residues in length, which correspond to an extra alpha helix and a beta strand when compared with TRX. TlpA catalyzes an essential reaction in the biogenesis of cytochrome aa3, while ResA and DsbE are essential proteins in cytochrome c maturation. Also included in this family are proteins containing a TlpA-like TRX domain with domain architectures similar to E. coli DipZ protein, and the N-terminal TRX domain of PilB protein from Neisseria which acts as a disulfide reductase that can recylce methionine sulfoxide reductases.


Pssm-ID: 239264 [Multi-domain]  Cd Length: 116  Bit Score: 97.31  E-value: 4.00e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDtlDNNEAGIKEAkeiLKAQ 247
Cdd:cd02966     2 FSLPDLDGKPVSLSDL-KGKVVLVNFWASWCPPCRAEMPELEALAKEYKDDGVEVVGVNVD--DDDPAAVKAF---LKKY 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1473338160 248 GASYKNLtFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:cd02966    76 GITFPVL-LDPDGELAKAYG---VRGLPTTFLIDRDGRIR 111
TrxA COG0526
Thiol-disulfide isomerase or thioredoxin [Posttranslational modification, protein turnover, ...
 168-314 1.60e-24

Thiol-disulfide isomerase or thioredoxin [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440292 [Multi-domain]  Cd Length: 139  Bit Score: 96.30  E-value: 1.60e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMggEVVGINMDtlDNNEAgikeAKEILKAQ 247
Cdd:COG0526    11 FTLTDLDGKPLSLADL-KGKPVLVNFWATWCPPCRAEMPVLKELAEEYGGV--VFVGVDVD--ENPEA----VKAFLKEL 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1473338160 248 GASYKNLtFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIVGEpFMGGIDdqsnYDQLMKQIQSVLDQ 314
Cdd:COG0526    82 GLPYPVL-LDPDGELAKAYG---VRGIPTTVLIDKDGKIVAR-HVGPLS----PEELEEALEKLLAK 139
Bcp COG1225
Peroxiredoxin [Posttranslational modification, protein turnover, chaperones];
168-308 4.58e-24

Peroxiredoxin [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440838 [Multi-domain]  Cd Length: 136  Bit Score: 94.93  E-value: 4.58e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVD-DSLfaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDTLDnneagikEAKEILKA 246
Cdd:COG1225     4 FTLPDLDGKTVSlSDL--RGKPVVLYFYATWCPGCTAELPELRDLYEEFKDKGVEVLGVSSDSDE-------AHKKFAEK 74

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1473338160 247 QGASYKNLtFDSNSTVGNyagKGNIMAFPTTVLVDKDGNIVGEpFMGGIDDQSNYDQLMKQI 308
Cdd:COG1225    75 YGLPFPLL-SDPDGEVAK---AYGVRGTPTTFLIDPDGKIRYV-WVGPVDPRPHLEEVLEAL 131
AhpC-TSA pfam00578
AhpC/TSA family; This family contains proteins related to alkyl hydroperoxide reductase (AhpC) ...
 168-287 8.11e-14

AhpC/TSA family; This family contains proteins related to alkyl hydroperoxide reductase (AhpC) and thiol specific antioxidant (TSA).


Pssm-ID: 425763 [Multi-domain]  Cd Length: 124  Bit Score: 66.86  E-value: 8.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVddSLFA-KNKVTVVNFW-FSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDTLDNneagikeAKEILK 245
Cdd:pfam00578   8 FELPDGDGGTV--SLSDyRGKWVVLFFYpADWTPVCTTELPALADLYEEFKKLGVEVLGVSVDSPES-------HKAFAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1473338160 246 AQGASYkNLTFDSNSTV-GNY-AGKGNIM-AFPTTVLVDKDGNIV 287
Cdd:pfam00578  79 KYGLPF-PLLSDPDGEVaRAYgVLNEEEGgALRATFVIDPDGKVR 122
Thioredoxin_8 pfam13905
Thioredoxin-like; Thioredoxins are small enzymes that participate in redox reactions, via the ...
 186-286 1.36e-12

Thioredoxin-like; Thioredoxins are small enzymes that participate in redox reactions, via the reversible oxidation of an active centre disulfide bond.


Pssm-ID: 464033 [Multi-domain]  Cd Length: 95  Bit Score: 62.71  E-value: 1.36e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 186 NKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGG-EVVGINMDTldnneaGIKEAKEILKAQGASYKNLTFDS--NSTV 262
Cdd:pfam13905   1 GKVVLLYFGASWCKPCRRFTPLLKELYEKLKKKKNvEIVFVSLDR------DLEEFKDYLKKMPKDWLSVPFDDdeRNEL 74

                          90       100
                  ....*....|....*....|....
gi 1473338160 263 GNYAgkgNIMAFPTTVLVDKDGNI 286
Cdd:pfam13905  75 KRKY---GVNAIPTLVLLDPNGEV 95
Redoxin pfam08534
Redoxin; This family of redoxins includes peroxiredoxin, thioredoxin and glutaredoxin proteins.
 168-287 1.36e-12

Redoxin; This family of redoxins includes peroxiredoxin, thioredoxin and glutaredoxin proteins.


Pssm-ID: 400717 [Multi-domain]  Cd Length: 148  Bit Score: 64.31  E-value: 1.36e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFS-GCKPCVGELSKLNELNEKLKEMGGEVVGINMdtlDNNEAGIKEAkeiLKA 246
Cdd:pfam08534  11 LPDAATDGNTVSLSDF-KGKKVVLNFWPGaFCPTCSAEHPYLEKLNELYKEKGVDVVAVNS---DNDAFFVKRF---WGK 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1473338160 247 QGASYKnLTFDSNSTVG-NY-----AGKGNIMAFPTTVLVDKDGNIV 287
Cdd:pfam08534  84 EGLPFP-FLSDGNAAFTkALglpieEDASAGLRSPRYAVIDEDGKVV 129
PRK03147 PRK03147
thiol-disulfide oxidoreductase ResA;
168-287 4.20e-10

thiol-disulfide oxidoreductase ResA;


Pssm-ID: 179545 [Multi-domain]  Cd Length: 173  Bit Score: 57.71  E-value: 4.20e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDtldnneagikEAKEILKAQ 247
Cdd:PRK03147   44 FVLTDLEGKKIELKDL-KGKGVFLNFWGTWCKPCEKEMPYMNELYPKYKEKGVEIIAVNVD----------ETELAVKNF 112

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1473338160 248 GASYkNLTF----DSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:PRK03147  113 VNRY-GLTFpvaiDKGRQVIDAYG---VGPLPTTFLIDKDGKVV 152
TlpA_like_DsbE cd03010
TlpA-like family, DsbE (also known as CcmG and CycY) subfamily; DsbE is a membrane-anchored, ...
 168-287 3.02e-09

TlpA-like family, DsbE (also known as CcmG and CycY) subfamily; DsbE is a membrane-anchored, periplasmic TRX-like reductase containing a CXXC motif that specifically donates reducing equivalents to apocytochrome c via CcmH, another cytochrome c maturation (Ccm) factor with a redox active CXXC motif. Assembly of cytochrome c requires the ligation of heme to reduced thiols of the apocytochrome. In bacteria, this assembly occurs in the periplasm. The reductase activity of DsbE in the oxidizing environment of the periplasm is crucial in the maturation of cytochrome c.


Pssm-ID: 239308 [Multi-domain]  Cd Length: 127  Bit Score: 54.12  E-value: 3.02e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGND--VDDSLFaKNKVTVVNFWFSGCKPCVGELSKLNELNeklKEMGGEVVGINMdtLDNNEAgikeAKEILK 245
Cdd:cd03010     6 FSLPALPGPDktLTSADL-KGKPYLLNVWASWCAPCREEHPVLMALA---RQGRVPIYGINY--KDNPEN----ALAWLA 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1473338160 246 AQGASYKNLTFDSNSTVGNYAGkgnIMAFPTTVLVDKDGNIV 287
Cdd:cd03010    76 RHGNPYAAVGFDPDGRVGIDLG---VYGVPETFLIDGDGIIR 114
PRX_like1 cd02969
Peroxiredoxin (PRX)-like 1 family; hypothetical proteins that show sequence similarity to PRXs. ...
 168-297 1.40e-04

Peroxiredoxin (PRX)-like 1 family; hypothetical proteins that show sequence similarity to PRXs. Members of this group contain a conserved cysteine that aligns to the first cysteine in the CXXC motif of TRX. This does not correspond to the peroxidatic cysteine found in PRXs, which aligns to the second cysteine in the CXXC motif of TRX. In addition, these proteins do not contain the other two conserved residues of the catalytic triad of PRX. PRXs confer a protective antioxidant role in cells through their peroxidase activity in which hydrogen peroxide, peroxynitrate, and organic hydroperoxides are reduced and detoxified using reducing equivalents derived from either thioredoxin, glutathione, trypanothione and AhpF.


Pssm-ID: 239267 [Multi-domain]  Cd Length: 171  Bit Score: 41.84  E-value: 1.40e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDDSLFAKNKVTVVNFWFSGCkPCV-GELSKLNELNEKLKEMGGEVVGINM-DTLDNNEAGIKEAKEILK 245
Cdd:cd02969     7 FSLPDTDGKTYSLADFADGKALVVMFICNHC-PYVkAIEDRLNRLAKEYGAKGVAVVAINSnDIEAYPEDSPENMKAKAK 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1473338160 246 AQGASYKNLtFDSNSTVGNYAGKgniMAFPTTVLVDKDGNIVgepFMGGIDD 297
Cdd:cd02969    86 EHGYPFPYL-LDETQEVAKAYGA---ACTPDFFLFDPDGKLV---YRGRIDD 130
TryX_like_TryX_NRX cd03009
Tryparedoxin (TryX)-like family, TryX and nucleoredoxin (NRX) subfamily; TryX and NRX are ...
 174-289 4.80e-04

Tryparedoxin (TryX)-like family, TryX and nucleoredoxin (NRX) subfamily; TryX and NRX are thioredoxin (TRX)-like protein disulfide oxidoreductases that alter the redox state of target proteins via the reversible oxidation of an active center CXXC motif. TryX is involved in the regulation of oxidative stress in parasitic trypanosomatids by reducing TryX peroxidase, which in turn catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. TryX derives reducing equivalents from reduced trypanothione, a polyamine peptide conjugate unique to trypanosomatids, which is regenerated by the NADPH-dependent flavoprotein trypanothione reductase. Vertebrate NRX is a 400-amino acid nuclear protein with one redox active TRX domain containing a CPPC active site motif followed by one redox inactive TRX-like domain. Mouse NRX transcripts are expressed in all adult tissues but is restricted to the nervous system and limb buds in embryos. Plant NRX, longer than the vertebrate NRX by about 100-200 amino acids, is a nuclear protein containing a redox inactive TRX-like domain between two redox active TRX domains. Both vertebrate and plant NRXs show thiol oxidoreductase activity in vitro. Their localization in the nucleus suggests a role in the redox regulation of nuclear proteins such as transcription factors.


Pssm-ID: 239307 [Multi-domain]  Cd Length: 131  Bit Score: 39.58  E-value: 4.80e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 174 DGNDVD-DSLFAKNkvtvVNFWFSG--CKPCVGELSKLNELNEKLKEMGG--EVVGInmdTLDNNEAGIKEakeilkaqg 248
Cdd:cd03009     7 DGGKVPvSSLEGKT----VGLYFSAswCPPCRAFTPKLVEFYEKLKESGKnfEIVFI---SWDRDEESFND--------- 70

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 1473338160 249 aSYKN-----LTFDSNSTVGNYAGKGNIMAFPTTVLVDKDGNIVGE 289
Cdd:cd03009    71 -YFSKmpwlaVPFSDRERRSRLNRTFKIEGIPTLIILDADGEVVTT 115
PRX_like2 cd02970
Peroxiredoxin (PRX)-like 2 family; hypothetical proteins that show sequence similarity to PRXs. ...
 171-287 5.48e-04

Peroxiredoxin (PRX)-like 2 family; hypothetical proteins that show sequence similarity to PRXs. Members of this group contain a CXXC motif, similar to TRX. The second cysteine in the motif corresponds to the peroxidatic cysteine of PRX, however, these proteins do not contain the other two residues of the catalytic triad of PRX. PRXs confer a protective antioxidant role in cells through their peroxidase activity in which hydrogen peroxide, peroxynitrate, and organic hydroperoxides are reduced and detoxified using reducing equivalents derived from either thioredoxin, glutathione, trypanothione and AhpF. TRXs alter the redox state of target proteins by catalyzing the reduction of their disulfide bonds via the CXXC motif using reducing equivalents derived from either NADPH or ferredoxins.


Pssm-ID: 239268 [Multi-domain]  Cd Length: 149  Bit Score: 39.65  E-value: 5.48e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 171 KDLDGNDVDDSLFAKNKVTVVNFW-FSGCKPCVGELSKLNELNEKLKEMGGEVVGINMDTLDNNEAGIKE---------- 239
Cdd:cd02970     8 PDAGGETVTLSALLGEGPVVVVFYrGFGCPFCREYLRALSKLLPELDALGVELVAVGPESPEKLEAFDKGkflpfpvyad 87

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1473338160 240 -----AKE--ILKAQGASYKNLTFDSNSTVGNYAGK-GNIMAFPTTVLVDKDGNIV 287
Cdd:cd02970    88 pdrklYRAlgLVRSLPWSNTPRALWKNAAIGFRGNDeGDGLQLPGVFVIGPDGTIL 143
TlpA_like_ScsD_MtbDsbE cd03011
TlpA-like family, suppressor for copper sensitivity D protein (ScsD) and actinobacterial DsbE ...
 168-285 3.41e-03

TlpA-like family, suppressor for copper sensitivity D protein (ScsD) and actinobacterial DsbE homolog subfamily; composed of ScsD, the DsbE homolog of Mycobacterium tuberculosis (MtbDsbE) and similar proteins, all containing a redox-active CXXC motif. The Salmonella typhimurium ScsD is a thioredoxin-like protein which confers copper tolerance to copper-sensitive mutants of E. coli. MtbDsbE has been characterized as an oxidase in vitro, catalyzing the disulfide bond formation of substrates like hirudin. The reduced form of MtbDsbE is more stable than its oxidized form, consistent with an oxidase function. This is in contrast to the function of DsbE from gram-negative bacteria which is a specific reductase of apocytochrome c.


Pssm-ID: 239309 [Multi-domain]  Cd Length: 123  Bit Score: 36.89  E-value: 3.41e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473338160 168 FKGKDLDGNDVDdSLFAKNKVTVVNFWFSGCKPCVGELSKLNELNEKLKemggeVVGINMDTLDNNEAgikeakeilkAQ 247
Cdd:cd03011     3 FTATTLDGEQFD-LESLSGKPVLVYFWATWCPVCRFTSPTVNQLAADYP-----VVSVALRSGDDGAV----------AR 66

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1473338160 248 GASYKNLTFDS-NSTVGNYAGKGNIMAFPTTVLVDKDGN 285
Cdd:cd03011    67 FMQKKGYGFPViNDPDGVISARWGVSVTPAIVIVDPGGI 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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