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Conserved domains on  [gi|1450161760|ref|WP_116225458|]
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PTS sugar transporter subunit IIB [Pelolinea submarina]

Protein Classification

PTS sugar transporter subunit IIB( domain architecture ID 10007338)

PTS sugar transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming substrates (such as ascorbate, lactose, galactitol) concomitantly with their translocation across the cell membrane

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 3-96 2.80e-22

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


:

Pssm-ID: 442640  Cd Length: 94  Bit Score: 82.91  E-value: 2.80e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   3 KVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETNKPVFNAIPLITGVGED 82
Cdd:COG3414     1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADDADLIVTTTDLAEELGIPVINVIPLLNGIDKD 80

                          90
                  ....*....|....
gi 1450161760  83 ELVEKIIVKVKEIQ 96
Cdd:COG3414    81 EIKEKLLEALKELG 94
 
Name Accession Description Interval E-value
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 3-96 2.80e-22

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 82.91  E-value: 2.80e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   3 KVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETNKPVFNAIPLITGVGED 82
Cdd:COG3414     1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADDADLIVTTTDLAEELGIPVINVIPLLNGIDKD 80

                          90
                  ....*....|....
gi 1450161760  83 ELVEKIIVKVKEIQ 96
Cdd:COG3414    81 EIKEKLLEALKELG 94
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 5-89 6.71e-19

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 74.11  E-value: 6.71e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   5 KIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETNKPVFNAIPLITGVGEDEL 84
Cdd:cd05566     2 KILVACGTGVATSTVVASKVKELLKENGIDVKVEQCKIAEVPSLLDDADLIVSTTKVPEDYGIPVINGLPFLTGIGEDKV 81


                  ....*
gi 1450161760  85 VEKII 89
Cdd:cd05566    82 YEEIL 86
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
3-90 2.56e-06

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 42.16  E-value: 2.56e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   3 KVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVE-TNKPVFNAIPLITGVGE 81
Cdd:PRK10310    2 KRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTARVDRSfGDIPLVHGMPFVSGVGI 81


                  ....*....
gi 1450161760  82 DELVEKIIV 90
Cdd:PRK10310   82 EALQNKILT 90
 
Name Accession Description Interval E-value
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 3-96 2.80e-22

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 82.91  E-value: 2.80e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   3 KVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETNKPVFNAIPLITGVGED 82
Cdd:COG3414     1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADDADLIVTTTDLAEELGIPVINVIPLLNGIDKD 80

                          90
                  ....*....|....
gi 1450161760  83 ELVEKIIVKVKEIQ 96
Cdd:COG3414    81 EIKEKLLEALKELG 94
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 5-89 6.71e-19

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 74.11  E-value: 6.71e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   5 KIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETNKPVFNAIPLITGVGEDEL 84
Cdd:cd05566     2 KILVACGTGVATSTVVASKVKELLKENGIDVKVEQCKIAEVPSLLDDADLIVSTTKVPEDYGIPVINGLPFLTGIGEDKV 81


                  ....*
gi 1450161760  85 VEKII 89
Cdd:cd05566    82 YEEIL 86
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 5-88 3.20e-08

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 46.48  E-value: 3.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   5 KIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELpGRIANYDIVVSNVQV-TVETNKPVFNAIPLITGVGEDE 83
Cdd:cd00133     1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEAQGLSEV-IDLADADLIISTVPLaARFLGKPVIVVSPLLNEKDGEK 79


                  ....*
gi 1450161760  84 LVEKI 88
Cdd:cd00133    80 ILEKL 84
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
3-90 2.56e-06

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 42.16  E-value: 2.56e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   3 KVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVE-TNKPVFNAIPLITGVGE 81
Cdd:PRK10310    2 KRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTARVDRSfGDIPLVHGMPFVSGVGI 81


                  ....*....
gi 1450161760  82 DELVEKIIV 90
Cdd:PRK10310   82 EALQNKILT 90
BglG COG3711
Transcriptional antiterminator [Transcription];
1-96 1.80e-04

Transcriptional antiterminator [Transcription];


Pssm-ID: 442925 [Multi-domain]  Cd Length: 618  Bit Score: 38.69  E-value: 1.80e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   1 MYKVKIVVVCGSGVAMAMHAAYKLKEKLeaEKLSVHIDGSGNNELPGRIANYDIVVSNVQVtveTNKPVFNAIPLITgvg 80
Cdd:COG3711   389 SKKKRVLVVCSSGIGTSRLLKSRLKKLF--PEIEIIDVISYRELEEIDLEDYDLIISTVPL---EDKPVIVVSPLLT--- 460

                          90
                  ....*....|....*.
gi 1450161760  81 eDELVEKIIVKVKEIQ 96
Cdd:COG3711   461 -EEDIEKIRKFLKQIK 475
PTS_IIB_mannitol cd05567
PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate: ...
 5-88 5.16e-04

PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a mannitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIA, IIB, and IIC domains are expressed from the mtlA gene as a single protein, also known as the mannitol PTS permease, the mtl transporter, or MtlA. MtlA is only functional as a dimer with the dimer contacts occuring between the IIC domains. MtlA takes up exogenous mannitol releasing the phosphate ester into the cytoplasm in preparation for oxidation to fructose-6-phosphate by the NAD-dependent mannitol-P dehydrogenase (MtlD). The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include mannitol, chitobiose/lichenan, ascorbate, lactose, galactitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99909 [Multi-domain]  Cd Length: 87  Bit Score: 35.62  E-value: 5.16e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   5 KIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPgriANYDIVVSNVQVTVE-----TNKPVFNAIPLITGV 79
Cdd:cd05567     2 KIVFACDAGMGSSAMGASVLRKKLKKAGLEIPVTNSAIDELP---SDADLVVTHASLTDRakkkaPQAQHLSVDNFLNTP 78


                  ....*....
gi 1450161760  80 GEDELVEKI 88
Cdd:cd05567    79 EYDELIERL 87
FrwC COG1299
Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and ...
 1-101 2.54e-03

Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and metabolism];


Pssm-ID: 440910 [Multi-domain]  Cd Length: 469  Bit Score: 35.25  E-value: 2.54e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1450161760   1 MYKVKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHI--------DGSGNNELPGRIANYDIVVSNVQVtVETNKPVFNA 72
Cdd:COG1299     4 SKKKVIVVATTTAGATAAAAAAAAAAAAAAAAGGVIVvvvgggggGGGNLLTTEEEAAAAAIIIAAADD-VEDRFFGKKG 82

                          90       100
                  ....*....|....*....|....*....
gi 1450161760  73 IPLITGVGEDELVEKIIVKVKEIQAEKSA 101
Cdd:COG1299    83 KVVVVVAGGAAKKELLIIAAAAAAAAVYA 111
PRK09548 PRK09548
PTS ascorbate-specific subunit IIBC;
4-66 6.23e-03

PTS ascorbate-specific subunit IIBC;


Pssm-ID: 236559 [Multi-domain]  Cd Length: 602  Bit Score: 34.33  E-value: 6.23e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1450161760   4 VKIVVVCGSGVAMAMHAAYKLKEKLEAEKLSVHIDGSGNNELPGRIANYDIVVSNVQVTVETN 66
Cdd:PRK09548  507 VRILAVCGQGQGSSMMMKMKIKKYLDKRGIPIIMDSCAVNDYKGKLETIDIIVCSKHLANEIE 569
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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