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Conserved domains on  [gi|1372008135|ref|WP_106885821|]
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MULTISPECIES: sigma-E factor negative regulatory protein [Aeromonas]

Protein Classification

sigma-E factor negative regulatory protein( domain architecture ID 11459655)

sigma-E factor negative regulatory protein similar to anti-sigma-E factor RseA that functions as the anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-E (RpoE); ECF sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RseA COG3073
RseA, negative regulator of sigma E activity [Signal transduction mechanisms];
1-184 3.90e-47

RseA, negative regulator of sigma E activity [Signal transduction mechanisms];


:

Pssm-ID: 442307  Cd Length: 166  Bit Score: 152.06  E-value: 3.90e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   1 MANKEQLSALMDGDLSDME---VLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPVQ 77
Cdd:COG3073     3 EMQKEQLSALMDGELDELEdrrLLDALAQDAELRQTWARYHLIGDVLRGELLAVLSLDFAARVAAALADEPTVLAPAAAP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135  78 PVAPqvkpagkvvplIRRFGQQMGQYAIAASVAAAVIFGVQQYQGNDGmpANPVLNTIPVGGSATPVSVHYPqegnratp 157
Cdd:COG3073    83 EARK-----------LRRWLRPLGQVAVAASVALVAVVGVQQYNGPGD--AAPVLATVPVSGSASPVSLAVP-------- 141

                         170       180
                  ....*....|....*....|....*..
gi 1372008135 158 rqqglneQQMQEQRERINAFLRDHQLQ 184
Cdd:COG3073   142 -------SQVLEQDRRLNAYLQAHQQR 161
 
Name Accession Description Interval E-value
RseA COG3073
RseA, negative regulator of sigma E activity [Signal transduction mechanisms];
1-184 3.90e-47

RseA, negative regulator of sigma E activity [Signal transduction mechanisms];


Pssm-ID: 442307  Cd Length: 166  Bit Score: 152.06  E-value: 3.90e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   1 MANKEQLSALMDGDLSDME---VLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPVQ 77
Cdd:COG3073     3 EMQKEQLSALMDGELDELEdrrLLDALAQDAELRQTWARYHLIGDVLRGELLAVLSLDFAARVAAALADEPTVLAPAAAP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135  78 PVAPqvkpagkvvplIRRFGQQMGQYAIAASVAAAVIFGVQQYQGNDGmpANPVLNTIPVGGSATPVSVHYPqegnratp 157
Cdd:COG3073    83 EARK-----------LRRWLRPLGQVAVAASVALVAVVGVQQYNGPGD--AAPVLATVPVSGSASPVSLAVP-------- 141

                         170       180
                  ....*....|....*....|....*..
gi 1372008135 158 rqqglneQQMQEQRERINAFLRDHQLQ 184
Cdd:COG3073   142 -------SQVLEQDRRLNAYLQAHQQR 161
PRK10863 PRK10863
anti-sigma-E factor RseA;
4-190 4.42e-47

anti-sigma-E factor RseA;


Pssm-ID: 182789  Cd Length: 216  Bit Score: 153.67  E-value: 4.42e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   4 KEQLSALMDGDLSDMEVLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPVQPVAPQV 83
Cdd:PRK10863    3 KEKLSALMDGETLDSELLNALSHDPEMQKTWESYHLIRDTLRGDVGEVLHFDIASRVAAAIENEPVRQVPTLIPESQPAP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135  84 KPAGKvVPL---IRRFGQQMGQYAIAASVAAAVIFGVQQYQGN---DGMPANPVLNTIPVGGSATPVSVHYPQEGNRATP 157
Cdd:PRK10863   83 HQWQK-MPFwqkVRPWASQLTQMGVAACVSLAVIVGVQHYNGQsetSQQPETPVFNTLPMMGKASPVSLGVPSEAAAGNG 161

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1372008135 158 RQqglneQQMQEQRERINAFLRDHQLQQRLLQD 190
Cdd:PRK10863  162 QQ-----QQVQEQRRRINAMLQDYELQRRLHSE 189
RseA_N pfam03872
Anti sigma-E protein RseA, N-terminal domain; Sigma-E is important for the induction of ...
 4-84 3.30e-34

Anti sigma-E protein RseA, N-terminal domain; Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters. The C-terminal domain is located in the periplasm, and may interact with other protein that signal periplasmic stress.


Pssm-ID: 427557  Cd Length: 87  Bit Score: 116.59  E-value: 3.30e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   4 KEQLSALMDGDLSDMEVLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPV---QPVA 80
Cdd:pfam03872   3 KEQLSALMDGELLDSELLNALSQDAELQQTWARYHLIRDVLRGELPVVLGADIAARVAAALEDEPVHLVPRVApesQPAP 82


                  ....
gi 1372008135  81 PQVK 84
Cdd:pfam03872  83 RQWK 86
RseA_N cd16328
N-terminal domain of RseA; This family contains the cytoplasmic (N-terminal) domain of RseA, ...
 4-65 1.78e-25

N-terminal domain of RseA; This family contains the cytoplasmic (N-terminal) domain of RseA, the transmembrane anti-sigma-E factor. RseA is degraded during sigma-E-dependent transcription caused by bacterial envelope stress such as heat shock. It is an inner membrane protein with an N-terminal cytoplasmic domain that binds sigma-E and blocks its transcriptional activity, and a C-terminal periplasmic domain that binds RseB, an auxiliary negative regulator. Under inducing conditions, RseA is rapidly degraded and sigma-E is released into the cytoplasm, where it can bind core RNAP and induce its regulon. It has been shown that just the N-terminal domain is sufficient to bind and inhibit sigma-E. The C-terminal domain may interact with other proteins that signal periplasmic stress.


Pssm-ID: 319992  Cd Length: 65  Bit Score: 93.41  E-value: 1.78e-25
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1372008135   4 KEQLSALMDGDLSDME---VLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALE 65
Cdd:cd16328     1 KEQLSALMDGELDELEldrLLADLDSDPELRATWQRYHLIGDVLRGELLAAASADFAARVAAALA 65
 
Name Accession Description Interval E-value
RseA COG3073
RseA, negative regulator of sigma E activity [Signal transduction mechanisms];
1-184 3.90e-47

RseA, negative regulator of sigma E activity [Signal transduction mechanisms];


Pssm-ID: 442307  Cd Length: 166  Bit Score: 152.06  E-value: 3.90e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   1 MANKEQLSALMDGDLSDME---VLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPVQ 77
Cdd:COG3073     3 EMQKEQLSALMDGELDELEdrrLLDALAQDAELRQTWARYHLIGDVLRGELLAVLSLDFAARVAAALADEPTVLAPAAAP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135  78 PVAPqvkpagkvvplIRRFGQQMGQYAIAASVAAAVIFGVQQYQGNDGmpANPVLNTIPVGGSATPVSVHYPqegnratp 157
Cdd:COG3073    83 EARK-----------LRRWLRPLGQVAVAASVALVAVVGVQQYNGPGD--AAPVLATVPVSGSASPVSLAVP-------- 141

                         170       180
                  ....*....|....*....|....*..
gi 1372008135 158 rqqglneQQMQEQRERINAFLRDHQLQ 184
Cdd:COG3073   142 -------SQVLEQDRRLNAYLQAHQQR 161
PRK10863 PRK10863
anti-sigma-E factor RseA;
4-190 4.42e-47

anti-sigma-E factor RseA;


Pssm-ID: 182789  Cd Length: 216  Bit Score: 153.67  E-value: 4.42e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   4 KEQLSALMDGDLSDMEVLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPVQPVAPQV 83
Cdd:PRK10863    3 KEKLSALMDGETLDSELLNALSHDPEMQKTWESYHLIRDTLRGDVGEVLHFDIASRVAAAIENEPVRQVPTLIPESQPAP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135  84 KPAGKvVPL---IRRFGQQMGQYAIAASVAAAVIFGVQQYQGN---DGMPANPVLNTIPVGGSATPVSVHYPQEGNRATP 157
Cdd:PRK10863   83 HQWQK-MPFwqkVRPWASQLTQMGVAACVSLAVIVGVQHYNGQsetSQQPETPVFNTLPMMGKASPVSLGVPSEAAAGNG 161

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1372008135 158 RQqglneQQMQEQRERINAFLRDHQLQQRLLQD 190
Cdd:PRK10863  162 QQ-----QQVQEQRRRINAMLQDYELQRRLHSE 189
RseA_N pfam03872
Anti sigma-E protein RseA, N-terminal domain; Sigma-E is important for the induction of ...
 4-84 3.30e-34

Anti sigma-E protein RseA, N-terminal domain; Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters. The C-terminal domain is located in the periplasm, and may interact with other protein that signal periplasmic stress.


Pssm-ID: 427557  Cd Length: 87  Bit Score: 116.59  E-value: 3.30e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1372008135   4 KEQLSALMDGDLSDMEVLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALEDEPVILAPKPV---QPVA 80
Cdd:pfam03872   3 KEQLSALMDGELLDSELLNALSQDAELQQTWARYHLIRDVLRGELPVVLGADIAARVAAALEDEPVHLVPRVApesQPAP 82


                  ....
gi 1372008135  81 PQVK 84
Cdd:pfam03872  83 RQWK 86
RseA_N cd16328
N-terminal domain of RseA; This family contains the cytoplasmic (N-terminal) domain of RseA, ...
 4-65 1.78e-25

N-terminal domain of RseA; This family contains the cytoplasmic (N-terminal) domain of RseA, the transmembrane anti-sigma-E factor. RseA is degraded during sigma-E-dependent transcription caused by bacterial envelope stress such as heat shock. It is an inner membrane protein with an N-terminal cytoplasmic domain that binds sigma-E and blocks its transcriptional activity, and a C-terminal periplasmic domain that binds RseB, an auxiliary negative regulator. Under inducing conditions, RseA is rapidly degraded and sigma-E is released into the cytoplasm, where it can bind core RNAP and induce its regulon. It has been shown that just the N-terminal domain is sufficient to bind and inhibit sigma-E. The C-terminal domain may interact with other proteins that signal periplasmic stress.


Pssm-ID: 319992  Cd Length: 65  Bit Score: 93.41  E-value: 1.78e-25
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1372008135   4 KEQLSALMDGDLSDME---VLNELGTDPALQDTWARYHLIGDAMRGDLPVNLQLDLSDSIMLALE 65
Cdd:cd16328     1 KEQLSALMDGELDELEldrLLADLDSDPELRATWQRYHLIGDVLRGELLAAASADFAARVAAALA 65
RseA_C pfam03873
Anti sigma-E protein RseA, C-terminal domain; Sigma-E is important for the induction of ...
 130-187 1.42e-14

Anti sigma-E protein RseA, C-terminal domain; Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters. The C-terminal domain is located in the periplasm, and may interact with other protein that signal periplasmic stress.


Pssm-ID: 427558  Cd Length: 53  Bit Score: 64.98  E-value: 1.42e-14
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1372008135 130 PVLNTIPVGGSATPVSVHYPQEgnratprQQGLNEQQMQEQRERINAFLRDHQLQQRL 187
Cdd:pfam03873   3 PVLQTLPFMGSASPVSYNVPQE-------QPAVTAEQLQEQRRRINAYLQDHELQRRL 53
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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