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Conserved domains on  [gi|1271479712|ref|WP_099265323.1|]
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HlyD family secretion protein

Protein Classification

HlyD family secretion protein( domain architecture ID 11446287)

HlyD family secretion protein similar to Escherichia coli protein YhiI, Acinetobacter baumannii colistin resistance protein EmrA, and Burkholderia cepacia fusaric acid resistance protein FusE

Gene Ontology:  GO:0022857|GO:0016020|GO:0055085
TCDB:  3.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10559 super family cl32535
p-hydroxybenzoic acid efflux pump subunit AaeA;
2-281 8.86e-73

p-hydroxybenzoic acid efflux pump subunit AaeA;


The actual alignment was detected with superfamily member PRK10559:

Pssm-ID: 182548 [Multi-domain]  Cd Length: 310  Bit Score: 225.78  E-value: 8.86e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRI 81
Cdd:PRK10559    6 RKISRTAITLVLVILAFIAIFRAWVFYTESPWTRDARFSADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQK 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  82 AVKQAQALLASRKATWEMRKLNARRRADMDELVISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYV 161
Cdd:PRK10559   86 ALAEAEADVAYYQVLAQEKRREAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPADGWV 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 162 TNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPADLQ-LMSGEVLKGHVESIARGIYDRDNPQSRELI 240
Cdd:PRK10559  166 TNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITpLGSNKVLKGTVDSVAAGVTNSSSTRDSKGM 245

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|.
gi 1271479712 241 ADVNPTFNWVRLAQRVPVRIHLDQVPQDvLLAAGMTCTVIV 281
Cdd:PRK10559  246 ATIDSNLEWVRLAQRVPVRIRLDNQQGN-LYPAGTTATVVI 285
 
Name Accession Description Interval E-value
PRK10559 PRK10559
p-hydroxybenzoic acid efflux pump subunit AaeA;
2-281 8.86e-73

p-hydroxybenzoic acid efflux pump subunit AaeA;


Pssm-ID: 182548 [Multi-domain]  Cd Length: 310  Bit Score: 225.78  E-value: 8.86e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRI 81
Cdd:PRK10559    6 RKISRTAITLVLVILAFIAIFRAWVFYTESPWTRDARFSADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQK 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  82 AVKQAQALLASRKATWEMRKLNARRRADMDELVISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYV 161
Cdd:PRK10559   86 ALAEAEADVAYYQVLAQEKRREAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPADGWV 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 162 TNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPADLQ-LMSGEVLKGHVESIARGIYDRDNPQSRELI 240
Cdd:PRK10559  166 TNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITpLGSNKVLKGTVDSVAAGVTNSSSTRDSKGM 245

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|.
gi 1271479712 241 ADVNPTFNWVRLAQRVPVRIHLDQVPQDvLLAAGMTCTVIV 281
Cdd:PRK10559  246 ATIDSNLEWVRLAQRVPVRIRLDNQQGN-LYPAGTTATVVI 285
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
1-283 1.21e-60

Multidrug resistance efflux pump EmrA [Defense mechanisms];


Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 195.27  E-value: 1.21e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   1 MK--KFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEH 78
Cdd:COG1566     1 MKalKKRRLLALVLLLLALGLALWAAGRNGPDEPVTADGRVEARVVTVAAKVSGRVTEVLVKEGDRVKKGQVLARLDPTD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  79 YRIAVKQAQALLASRKATWEMRKLNARRRADMDEL----------------------------VISAKNRDDASNVATSA 130
Cdd:COG1566    81 LQAALAQAEAQLAAAEAQLARLEAELGAEAEIAAAeaqlaaaqaqldlaqreleryqalykkgAVSQQELDEARAALDAA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 131 LADYQLAQAQLEA---------------------------AELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVD 183
Cdd:COG1566   161 QAQLEAAQAQLAQaqaglreeeelaaaqaqvaqaeaalaqAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVP 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 184 MNSFWVYGFFEETKLPHLKVGDPADLQLMS--GEVLKGHVESIARGIYDRDNPQsreliadvNPTFNwvrLAQRVPVRIH 261
Cdd:COG1566   241 LDDLWVEAYVPETDLGRVKPGQPVEVRVDAypDRVFEGKVTSISPGAGFTSPPK--------NATGN---VVQRYPVRIR 309

                         330       340
                  ....*....|....*....|..
gi 1271479712 262 LDQvPQDVLLAAGMTCTVIVRP 283
Cdd:COG1566   310 LDN-PDPEPLRPGMSATVEIDT 330
8a0101 TIGR00998
efflux pump membrane protein (multidrug resistance protein A); [Transport and binding proteins, ...
 2-279 2.96e-44

efflux pump membrane protein (multidrug resistance protein A); [Transport and binding proteins, Other]


Pssm-ID: 273385 [Multi-domain]  Cd Length: 334  Bit Score: 153.03  E-value: 2.96e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRI 81
Cdd:TIGR00998   1 RKYFLLLLVVLLIVVAGAYAIYWFLVLRDYESTDDAYVKANQLQVSSQVSGSVIEVNVDDTDYVKQGDVLVRLDPTNAEL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  82 AVKQAQALLAS--RKATWEMRKLNA---------------------------RRRADMDELVISAKNRDDASNVATSALA 132
Cdd:TIGR00998  81 ALAKAEANLAAlvRQTKQLEITVQQlqakveslkikleqarekllqaeldlrRRVPLFKKGLISREELDHARKALLSAKA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 133 DY--------------------------QLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNS 186
Cdd:TIGR00998 161 ALnaaiqeqlnanqalvrgtplkkqpavQEAKERLKTAWLALKRTVIRAPFDGYVARRFVQVGQVVSPGQPLMAVVPAEQ 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 187 FWVYGFFEETKLPHLKVGDPADLQL-MSGE--VLKGHVESIARGiydrdNPQSRELIADVNPTFNWVRLAQRVPVRIHLD 263
Cdd:TIGR00998 241 MYVEANFKETQLKNVRIGQPVTIRSdLYGSdvVFEGKVTGISMG-----TGSAFSLLPAQNATGNWIKVVQRLPVRIKLD 315

                         330
                  ....*....|....*..
gi 1271479712 264 QVPQDV-LLAAGMTCTV 279
Cdd:TIGR00998 316 PKELDEhPLRIGLSAEV 332
CusB_dom_1 pfam00529
Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli ...
 31-282 1.03e-28

Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.


Pssm-ID: 425733 [Multi-domain]  Cd Length: 322  Bit Score: 111.75  E-value: 1.03e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  31 TPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATwEMRKLNARRRAD- 109
Cdd:pfam00529   8 VEAPGRVVVSGNAKAVQPQVSGIVTRVLVKEGDRVKAGDVLFQLDPTDYQAALDSAEAQLAKAQAQ-VARLQAELDRLQa 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 110 -MDELVISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAA-------------------------------- 156
Cdd:pfam00529  87 lESELAISRQDYDGATAQLRAAQAAVKAAQAQLAQAQIDLARRRVLAPiggisreslvtagalvaqaqanllatvaqldq 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 157 -------------------------------------------------VDGYVTNLNVHR-GDYARIGEAKMAVVDMNS 186
Cdd:pfam00529 167 iyvqitqsaaenqaevrselsgaqlqiaeaeaelklakldlerteirapVDGTVAFLSVTVdGGTVSAGLRLMFVVPEDN 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 187 FWVYGFFEETKLPHLKVGDPADLQLMS-GEVLKGHVESIARGIYdrdnpqsreliadvnptfnwvrlAQRVPVRIHLDQV 265
Cdd:pfam00529 247 LLVPGMFVETQLDQVRVGQPVLIPFDAfPQTKTGRFTGVVVGIS-----------------------PDTGPVRVVVDKA 303

                         330
                  ....*....|....*...
gi 1271479712 266 PQDV-LLAAGMTCTVIVR 282
Cdd:pfam00529 304 QGPYyPLRIGLSAGALVR 321
 
Name Accession Description Interval E-value
PRK10559 PRK10559
p-hydroxybenzoic acid efflux pump subunit AaeA;
2-281 8.86e-73

p-hydroxybenzoic acid efflux pump subunit AaeA;


Pssm-ID: 182548 [Multi-domain]  Cd Length: 310  Bit Score: 225.78  E-value: 8.86e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRI 81
Cdd:PRK10559    6 RKISRTAITLVLVILAFIAIFRAWVFYTESPWTRDARFSADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQK 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  82 AVKQAQALLASRKATWEMRKLNARRRADMDELVISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYV 161
Cdd:PRK10559   86 ALAEAEADVAYYQVLAQEKRREAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPADGWV 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 162 TNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPADLQ-LMSGEVLKGHVESIARGIYDRDNPQSRELI 240
Cdd:PRK10559  166 TNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITpLGSNKVLKGTVDSVAAGVTNSSSTRDSKGM 245

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|.
gi 1271479712 241 ADVNPTFNWVRLAQRVPVRIHLDQVPQDvLLAAGMTCTVIV 281
Cdd:PRK10559  246 ATIDSNLEWVRLAQRVPVRIRLDNQQGN-LYPAGTTATVVI 285
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
1-283 1.21e-60

Multidrug resistance efflux pump EmrA [Defense mechanisms];


Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 195.27  E-value: 1.21e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   1 MK--KFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEH 78
Cdd:COG1566     1 MKalKKRRLLALVLLLLALGLALWAAGRNGPDEPVTADGRVEARVVTVAAKVSGRVTEVLVKEGDRVKKGQVLARLDPTD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  79 YRIAVKQAQALLASRKATWEMRKLNARRRADMDEL----------------------------VISAKNRDDASNVATSA 130
Cdd:COG1566    81 LQAALAQAEAQLAAAEAQLARLEAELGAEAEIAAAeaqlaaaqaqldlaqreleryqalykkgAVSQQELDEARAALDAA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 131 LADYQLAQAQLEA---------------------------AELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVD 183
Cdd:COG1566   161 QAQLEAAQAQLAQaqaglreeeelaaaqaqvaqaeaalaqAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVP 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 184 MNSFWVYGFFEETKLPHLKVGDPADLQLMS--GEVLKGHVESIARGIYDRDNPQsreliadvNPTFNwvrLAQRVPVRIH 261
Cdd:COG1566   241 LDDLWVEAYVPETDLGRVKPGQPVEVRVDAypDRVFEGKVTSISPGAGFTSPPK--------NATGN---VVQRYPVRIR 309

                         330       340
                  ....*....|....*....|..
gi 1271479712 262 LDQvPQDVLLAAGMTCTVIVRP 283
Cdd:COG1566   310 LDN-PDPEPLRPGMSATVEIDT 330
PRK10476 PRK10476
multidrug transporter subunit MdtN;
11-283 1.75e-49

multidrug transporter subunit MdtN;


Pssm-ID: 182488 [Multi-domain]  Cd Length: 346  Bit Score: 167.13  E-value: 1.75e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  11 LLVLAIALWIGLTLWVDyMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALL 90
Cdd:PRK10476   17 AIVALAIVALVFVIWRT-DSAPSTDDAYIDADVVHVASEVGGRIVELAVTENQAVKKGDLLFRIDPRPYELTVAQAQADL 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  91 ASRKATWEMR-------------------------KLNARRRADMDELV----ISAKNRDDASNVATSALADYQLA---- 137
Cdd:PRK10476   96 ALADAQIMTTqrsvdaersnaasaneqveraranaKLATRTLERLEPLLakgyVSAQQVDQARTAQRDAEVSLNQAllqa 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 138 --------------------QAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFEETK 197
Cdd:PRK10476  176 qaaaaavggvdalvaqraarEAALAIAELHLEDTTVRAPFDGRVVGLKVSVGEFAAPMQPIFTLIDTDHWYAIANFRETD 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 198 LPHLKVGDPADLQLMS--GEVLKGHVESIARGIYDRDNPQSRELIADVNPTFNWVRLAQRVPVRIHLDQVPQDvLLAAGM 275
Cdd:PRK10476  256 LKNIRVGDCATVYSMIdrGRPFEGKVDSIGWGVLPDDGGNVPRGLPYVPRSINWVRVAQRFPVRIMLDKPDPE-LFRIGA 334


                  ....*...
gi 1271479712 276 TCTVIVRP 283
Cdd:PRK10476  335 SAVVELRP 342
8a0101 TIGR00998
efflux pump membrane protein (multidrug resistance protein A); [Transport and binding proteins, ...
 2-279 2.96e-44

efflux pump membrane protein (multidrug resistance protein A); [Transport and binding proteins, Other]


Pssm-ID: 273385 [Multi-domain]  Cd Length: 334  Bit Score: 153.03  E-value: 2.96e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRI 81
Cdd:TIGR00998   1 RKYFLLLLVVLLIVVAGAYAIYWFLVLRDYESTDDAYVKANQLQVSSQVSGSVIEVNVDDTDYVKQGDVLVRLDPTNAEL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  82 AVKQAQALLAS--RKATWEMRKLNA---------------------------RRRADMDELVISAKNRDDASNVATSALA 132
Cdd:TIGR00998  81 ALAKAEANLAAlvRQTKQLEITVQQlqakveslkikleqarekllqaeldlrRRVPLFKKGLISREELDHARKALLSAKA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 133 DY--------------------------QLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNS 186
Cdd:TIGR00998 161 ALnaaiqeqlnanqalvrgtplkkqpavQEAKERLKTAWLALKRTVIRAPFDGYVARRFVQVGQVVSPGQPLMAVVPAEQ 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 187 FWVYGFFEETKLPHLKVGDPADLQL-MSGE--VLKGHVESIARGiydrdNPQSRELIADVNPTFNWVRLAQRVPVRIHLD 263
Cdd:TIGR00998 241 MYVEANFKETQLKNVRIGQPVTIRSdLYGSdvVFEGKVTGISMG-----TGSAFSLLPAQNATGNWIKVVQRLPVRIKLD 315

                         330
                  ....*....|....*..
gi 1271479712 264 QVPQDV-LLAAGMTCTV 279
Cdd:TIGR00998 316 PKELDEhPLRIGLSAEV 332
AcrA COG0845
Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope ...
 37-271 2.11e-41

Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope biogenesis, Defense mechanisms];


Pssm-ID: 440606 [Multi-domain]  Cd Length: 324  Bit Score: 145.09  E-value: 2.11e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  37 GRVRAD-IINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATWEMRKLNARRRADM-DELV 114
Cdd:COG0845    16 GTVEARrEVEVRARVSGRVEEVLVDEGDRVKKGQVLARLDPPDLQAALAQAQAQLAAAQAQLELAKAELERYKALlKKGA 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 115 ISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFE 194
Cdd:COG0845    96 VSQQELDQAKAALDQAQAALAAAQAALEQARANLAYTTIRAPFDGVVGERNVEPGQLVSAGTPLFTIADLDPLEVEFDVP 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 195 ETKLPHLKVGDPADLQL--MSGEVLKGHVESIARGIydrdNPQSRELIADV---NPTFNWvRLAQRVPVRIHLDQVPQDV 269
Cdd:COG0845   176 ESDLARLKVGQPVTVTLdaGPGKTFEGKVTFIDPAV----DPATRTVRVRAelpNPDGLL-RPGMFVRVRIVLGERENAL 250


                  ..
gi 1271479712 270 LL 271
Cdd:COG0845   251 LV 252
CusB_dom_1 pfam00529
Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli ...
 31-282 1.03e-28

Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.


Pssm-ID: 425733 [Multi-domain]  Cd Length: 322  Bit Score: 111.75  E-value: 1.03e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  31 TPWTRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATwEMRKLNARRRAD- 109
Cdd:pfam00529   8 VEAPGRVVVSGNAKAVQPQVSGIVTRVLVKEGDRVKAGDVLFQLDPTDYQAALDSAEAQLAKAQAQ-VARLQAELDRLQa 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 110 -MDELVISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAA-------------------------------- 156
Cdd:pfam00529  87 lESELAISRQDYDGATAQLRAAQAAVKAAQAQLAQAQIDLARRRVLAPiggisreslvtagalvaqaqanllatvaqldq 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 157 -------------------------------------------------VDGYVTNLNVHR-GDYARIGEAKMAVVDMNS 186
Cdd:pfam00529 167 iyvqitqsaaenqaevrselsgaqlqiaeaeaelklakldlerteirapVDGTVAFLSVTVdGGTVSAGLRLMFVVPEDN 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 187 FWVYGFFEETKLPHLKVGDPADLQLMS-GEVLKGHVESIARGIYdrdnpqsreliadvnptfnwvrlAQRVPVRIHLDQV 265
Cdd:pfam00529 247 LLVPGMFVETQLDQVRVGQPVLIPFDAfPQTKTGRFTGVVVGIS-----------------------PDTGPVRVVVDKA 303

                         330
                  ....*....|....*...
gi 1271479712 266 PQDV-LLAAGMTCTVIVR 282
Cdd:pfam00529 304 QGPYyPLRIGLSAGALVR 321
RND_mfp TIGR01730
RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion ...
 37-225 9.08e-25

RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273776 [Multi-domain]  Cd Length: 322  Bit Score: 100.85  E-value: 9.08e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  37 GRVRA-DIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATWEMRKLNARRRADMDEL-V 114
Cdd:TIGR01730  19 GSLEAvDEADLAAEVAGKITKISVREGQKVKKGQVLARLDDDDYQLALQAALAQLAAAEAQLELAQRSFERAERLVKRnA 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 115 ISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFE 194
Cdd:TIGR01730  99 VSQADLDDAKAAVEAAQADLEAAKASLASAQLNLRYTEIRAPFDGTIGRRLVEVGAYVTAGQTLATIVDLDPLEADFSVP 178

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1271479712 195 ETKLPHLKVGDPAD--LQLMSGEVLKGHVESIA 225
Cdd:TIGR01730 179 ERDLPQLRRGQTLTveLDALPGEEFKGKLRFID 211
PRK15136 PRK15136
multidrug efflux MFS transporter periplasmic adaptor subunit EmrA;
34-264 5.14e-22

multidrug efflux MFS transporter periplasmic adaptor subunit EmrA;


Pssm-ID: 185090 [Multi-domain]  Cd Length: 390  Bit Score: 94.38  E-value: 5.14e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  34 TRDGRVRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASR--------------KATWEM 99
Cdd:PRK15136   52 TDDAYVAGNQVQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDAEQAFEKAKTALANSvrqthqlminskqyQANIEL 131

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 100 RK---------LNARR---RADM---DEL------VISAKNRDDA------SNVA---TSALAD---YQLAQAQLEAAEL 146
Cdd:PRK15136  132 QKtalaqaqsdLNRRVplgNANLigrEELqhardaVASAQAQLDVaiqqynANQAmilNTPLEDqpaVQQAATEVRNAWL 211

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 147 NLARTRVLAAVDGYVTNLNVHRGdyARI--GEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPADLQ---LMSGEVLKGHV 221
Cdd:PRK15136  212 ALQRTKIVSPMTGYVSRRSVQVG--AQIspTTPLMAVVPATNLWVDANFKETQLANMRIGQPATITsdiYGDDVVYTGKV 289

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|...
gi 1271479712 222 ESIargiyDRDNPQSRELIADVNPTFNWVRLAQRVPVRIHLDQ 264
Cdd:PRK15136  290 VGL-----DMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDA 327
HlyD_D23 pfam16576
Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and ...
 37-237 1.64e-14

Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.


Pssm-ID: 435440 [Multi-domain]  Cd Length: 214  Bit Score: 70.61  E-value: 1.64e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  37 GRVRAD---IINVAADVSGSVVDVPVHDN-QWVKRGDLLMQID-PEhyrIAVKQAQALLAsrkatwemrkLNARRRADMD 111
Cdd:pfam16576  10 GRVAYDerrLAHVHARVEGWIEKLYVNATgDPVKKGQPLAELYsPE---LVAAQQEYLLA----------LRSGDALSKS 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 112 ELVISAKNRddasnvatsaLADYQLAQAQLEAaelnLARTR-------VLAAVDGYVTNLNVHRGDYARIGEAKMAVVDM 184
Cdd:pfam16576  77 ELLRAARQR----------LRLLGMPEAQIAE----LERTGkvqptvtVYAPISGVVTELNVREGMYVQPGDTLFTIADL 142

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1271479712 185 NSFWVYGFFEETKLPHLKVGDPADLQL--MSGEVLKGHVESiargIYDRDNPQSR 237
Cdd:pfam16576 143 STVWVEADVPEQDLALVKVGQPAEVTLpaLPGKTFEGKVDY----IYPTLDPKTR 193
Biotin_lipoyl_2 pfam13533
Biotin-lipoyl like;
44-91 2.66e-13

Biotin-lipoyl like;


Pssm-ID: 433286  Cd Length: 50  Bit Score: 62.85  E-value: 2.66e-13
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1271479712  44 INVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLA 91
Cdd:pfam13533   3 VKIASPVSGKVVAVNVKEGQQVKKGDVLATLDSPELQLQLQQAEAQLA 50
PRK11556 PRK11556
MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;
41-182 5.00e-12

MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 183194 [Multi-domain]  Cd Length: 415  Bit Score: 65.58  E-value: 5.00e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  41 ADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATWemrkLNARRR-ADMDELV----I 115
Cdd:PRK11556   85 ANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPRPFKVALAQAQGQLAKDQATL----ANARRDlARYQQLAktnlV 160

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1271479712 116 SAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEAKMAVV 182
Cdd:PRK11556  161 SRQELDAQQALVSETEGTIKADEASVASAQLQLDYSRITAPISGRVGLKQVDVGNQISSGDTTGIVV 227
PRK03598 PRK03598
putative efflux pump membrane fusion protein; Provisional
 1-161 4.43e-10

putative efflux pump membrane fusion protein; Provisional


Pssm-ID: 235136 [Multi-domain]  Cd Length: 331  Bit Score: 59.21  E-value: 4.43e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   1 MKKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVraDI--INVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEH 78
Cdd:PRK03598    1 MKKKVVIGLAVVVLAAAVAGGWWWYQSRQDNGLTLYGNV--DIrtVNLGFRVGGRLASLAVDEGDAVKAGQVLGELDAAP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  79 YRIAVKQAQALLASRKATWEMRKLNARR------RADMDEL---------------------VISAKNRDDA-------- 123
Cdd:PRK03598   79 YENALMQAKANVSVAQAQLDLMLAGYRDeeiaqaRAAVKQAqaaydyaqnfynrqqglwksrTISANDLENArssrdqaq 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1271479712 124 --SNVATSALADY--------------QLAQAQ--LEAAELNLARTRVLAAVDGYV 161
Cdd:PRK03598  159 atLKSAQDKLSQYregnrpqdiaqakaSLAQAQaaLAQAELNLQDTELIAPSDGTI 214
PRK15030 PRK15030
multidrug efflux RND transporter periplasmic adaptor subunit AcrA;
37-177 1.25e-08

multidrug efflux RND transporter periplasmic adaptor subunit AcrA;


Pssm-ID: 184990 [Multi-domain]  Cd Length: 397  Bit Score: 55.49  E-value: 1.25e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  37 GRVRA-DIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATWEMRKLNARRRADM-DELV 114
Cdd:PRK15030   58 GRTSAyRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLlGTQY 137

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1271479712 115 ISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDYARIGEA 177
Cdd:PRK15030  138 ISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQA 200
PRK09578 PRK09578
MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;
46-159 3.63e-08

MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 169982 [Multi-domain]  Cd Length: 385  Bit Score: 54.03  E-value: 3.63e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  46 VAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATwEMRKLNARRRadMDELV----ISAKNRD 121
Cdd:PRK09578   66 VRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAAGALAKAEAA-HLAALDKRRR--YDDLVrdraVSERDYT 142

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1271479712 122 DASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDG 159
Cdd:PRK09578  143 EAVADERQAKAAVASAKAELARAQLQLDYATVTAPIDG 180
PRK11578 PRK11578
macrolide transporter subunit MacA; Provisional
 2-207 3.86e-08

macrolide transporter subunit MacA; Provisional


Pssm-ID: 183211 [Multi-domain]  Cd Length: 370  Bit Score: 53.62  E-value: 3.86e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   2 KKFFSLFVTLLVLAIALWIGLTLWVDYMDTPWTRDGRVRADI-----------INVAADVSGSVVDVPVHDNQWVKRGDL 70
Cdd:PRK11578    9 KRYLIALVIVLAGGITLWRILNAPVPTYQTLIVRPGDLQQSVlatgkldalrkVDVGAQVSGQLKTLSVAIGDKVKKDQL 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  71 LMQIDPEHYRIAVKQAQA----LLASR---KATWEMRKLNARRRADMDELviSAKNRDDASNVAT----------SALAD 133
Cdd:PRK11578   89 LGVIDPEQAENQIKEVEAtlmeLRAQRqqaEAELKLARVTLSRQQRLAKT--QAVSQQDLDTAATelavkqaqigTIDAQ 166

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1271479712 134 YQLAQAQLEAAELNLARTRVLAAVDGYVTNLNVHRGDY---ARIGEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPA 207
Cdd:PRK11578  167 IKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTviaAQQAPNILTLADMSTMLVKAQVSEADVIHLKPGQKA 243
HlyD_3 pfam13437
HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator ...
 152-233 2.36e-06

HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator HlyD proteins.


Pssm-ID: 433206 [Multi-domain]  Cd Length: 104  Bit Score: 45.43  E-value: 2.36e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712 152 RVLAAVDGYVTNLNVHRGDYARIGEAKMAVVDMNSFWVYGFFEETKLPHLKVGDPADLQLMSGE--VLKGHVESIARGIY 229
Cdd:pfam13437   1 TIRAPVDGVVAELNVEEGQVVQAGDPLATIVPPDRLLVEAFVPAADLGSLKKGQKVTLKLDPGSdyTLEGKVVRISPTVD 80


                  ....
gi 1271479712 230 DRDN 233
Cdd:pfam13437  81 PDTG 84
PRK09859 PRK09859
multidrug transporter subunit MdtE;
37-159 4.74e-06

multidrug transporter subunit MdtE;


Pssm-ID: 137559 [Multi-domain]  Cd Length: 385  Bit Score: 47.40  E-value: 4.74e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712  37 GR-VRADIINVAADVSGSVVDVPVHDNQWVKRGDLLMQIDPEHYRIAVKQAQALLASRKATWEMRKLNARRRAD-MDELV 114
Cdd:PRK09859   54 GRtVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASlLKTNY 133

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1271479712 115 ISAKNRDDASNVATSALADYQLAQAQLEAAELNLARTRVLAAVDG 159
Cdd:PRK09859  134 VSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITG 178
PLN03200 PLN03200
cellulose synthase-interactive protein; Provisional
 39-154 2.65e-03

cellulose synthase-interactive protein; Provisional


Pssm-ID: 215629 [Multi-domain]  Cd Length: 2102  Bit Score: 39.32  E-value: 2.65e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1271479712   39 VRADIINVAADVSGSVVDVPVhDNQWVKrgdlLMQIDPEhyRIAVKQAQALLASRKATWEMRKLNARRRADMDELVISAK 118
Cdd:PLN03200   632 VLADIFSSRQDLCESLATDEI-INPCIK----LLTNNTE--AVATQSARALAALSRSIKENRKVSYAAEDAIKPLIKLAK 704

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 1271479712  119 NRD-DASNVATSALA----DYQLAQAQLeAAELNLARTRVL 154
Cdd:PLN03200   705 SSSiEVAEQAVCALAnllsDPEVAAEAL-AEDIILPLTRVL 744
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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