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Conserved domains on  [gi|1222367780|ref|WP_090420213|]
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MULTISPECIES: tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ [Enterobacter]

Protein Classification

tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ( domain architecture ID 11487661)

tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-241 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


:

Pssm-ID: 185069  Cd Length: 245  Bit Score: 515.07  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114    1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  81 ARSRTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114   81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780 161 LATQEK--EIEPKEETAYPLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQK 238
Cdd:PRK15114  161 LATQENgePQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                  ...
gi 1222367780 239 NKE 241
Cdd:PRK15114  241 NKG 243
 
Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-241 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


Pssm-ID: 185069  Cd Length: 245  Bit Score: 515.07  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114    1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  81 ARSRTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114   81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780 161 LATQEK--EIEPKEETAYPLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQK 238
Cdd:PRK15114  161 LATQENgePQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                  ...
gi 1222367780 239 NKE 241
Cdd:PRK15114  241 NKG 243
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 2-240 7.78e-133

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 373.68  E-value: 7.78e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSA 81
Cdd:COG0565     1 LDNIRVVLVEPQHPGNIGAAARAMKNFGLSDLRLVNPRGWPDEEARAMASGADDVLENARVVDTLEEALADCDLVVGTTA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  82 RSRTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:COG0565    81 RPRELRKPVLTPREAAEELLARAAGGKVALVFGRERSGLTNEELALCDALVTIPTNPEYPSLNLAQAVQVVAYELRKAAL 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1222367780 162 ATQEKEIEPKEETAypLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQKNK 240
Cdd:COG0565   161 EAPPLPPPPEPEPA--TAEELEGLYEHLEELLDAIGFLDPEKPEKLMRRLRRLFGRARLTEREVRTLRGILRALERRLK 237
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 2-240 3.05e-122

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 346.69  E-value: 3.05e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSA 81
Cdd:TIGR00050   1 LENIRIVLVEPSHPGNIGSIARAMKNMGLTELRLVNPRVKLEEEAYALAAGARDILDNAKVVDTLDEALDDCDFVVGTSA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  82 RSRTLPWPMLDPRECGLKSVseAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:TIGR00050  81 RSRNLQRPMLTPRELAPKLV--AEKGKVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYELRMAWL 158

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1222367780 162 ATQEKeiepKEETAYPLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQKNK 240
Cdd:TIGR00050 159 AQQEN----LSEMPYATVDDLELLLEHLEETLLSLGFIPEHKPRQVMRKFRRLYGRAGLEKRELHILRGILRSIEKRIK 233
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 5-155 2.51e-77

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 230.07  E-value: 2.51e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQ-AIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSARS 83
Cdd:cd18093     1 IRVVLVEPQHPGNIGAVARAMKNFGLSDLRLVNPRCDDPEEaARALASGAEDVLENAKVYDTLEEALADCDLVVATTARP 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1222367780  84 RTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18093    81 RGLRRPLLTPRELAELLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-154 5.27e-33

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 116.48  E-value: 5.27e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   4 NIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKP--DSQAIALAAGASDVIgNAQIVDTLDEALAGCSL----VV 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSADpyNPKAIRASAGALFHL-PLVVVDNLEEALKDLKEagyaVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1222367780  78 GTSARSrtlpwpmldprecglKSVSEAE-QAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAY 154
Cdd:pfam00588  80 ATSLGG---------------KSLYEIDfPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
PRK15114 PRK15114
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
 1-241 0e+00

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional


Pssm-ID: 185069  Cd Length: 245  Bit Score: 515.07  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS 80
Cdd:PRK15114    1 MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNATIVDTLDEALAGCSLVVGTS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  81 ARSRTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160
Cdd:PRK15114   81 ARSRTLPWPMLDPRECGLKSVAEAANAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780 161 LATQEK--EIEPKEETAYPLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQK 238
Cdd:PRK15114  161 LATQENgePQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQ 240


                  ...
gi 1222367780 239 NKE 241
Cdd:PRK15114  241 NKG 243
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 2-240 7.78e-133

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 373.68  E-value: 7.78e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSA 81
Cdd:COG0565     1 LDNIRVVLVEPQHPGNIGAAARAMKNFGLSDLRLVNPRGWPDEEARAMASGADDVLENARVVDTLEEALADCDLVVGTTA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  82 RSRTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:COG0565    81 RPRELRKPVLTPREAAEELLARAAGGKVALVFGRERSGLTNEELALCDALVTIPTNPEYPSLNLAQAVQVVAYELRKAAL 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1222367780 162 ATQEKEIEPKEETAypLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQKNK 240
Cdd:COG0565   161 EAPPLPPPPEPEPA--TAEELEGLYEHLEELLDAIGFLDPEKPEKLMRRLRRLFGRARLTEREVRTLRGILRALERRLK 237
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 2-240 3.05e-122

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 346.69  E-value: 3.05e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   2 LQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSA 81
Cdd:TIGR00050   1 LENIRIVLVEPSHPGNIGSIARAMKNMGLTELRLVNPRVKLEEEAYALAAGARDILDNAKVVDTLDEALDDCDFVVGTSA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  82 RSRTLPWPMLDPRECGLKSVseAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWL 161
Cdd:TIGR00050  81 RSRNLQRPMLTPRELAPKLV--AEKGKVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYELRMAWL 158

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1222367780 162 ATQEKeiepKEETAYPLVDDLERFYGHLEQTLLSTGFIREGHPGQVMNKLRRMFTRARPESQELNILRGILASIEQKNK 240
Cdd:TIGR00050 159 AQQEN----LSEMPYATVDDLELLLEHLEETLLSLGFIPEHKPRQVMRKFRRLYGRAGLEKRELHILRGILRSIEKRIK 233
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 5-155 2.51e-77

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 230.07  E-value: 2.51e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQ-AIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSARS 83
Cdd:cd18093     1 IRVVLVEPQHPGNIGAVARAMKNFGLSDLRLVNPRCDDPEEaARALASGAEDVLENAKVYDTLEEALADCDLVVATTARP 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1222367780  84 RTLPWPMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18093    81 RGLRRPLLTPRELAELLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
PRK10433 PRK10433
putative RNA methyltransferase; Provisional
 5-175 1.36e-39

putative RNA methyltransferase; Provisional


Pssm-ID: 236693  Cd Length: 228  Bit Score: 136.27  E-value: 1.36e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   5 IRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTSARSR 84
Cdd:PRK10433    3 LTIILVAPARAENVGAAARAMKTMGFSELRIVDSQAHLEPAARWVAHGSGDILDNAKVFDTLAEALHDVDFTVATTARSR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  85 TLPWPMLDPREC-GLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEvrmawLAT 163
Cdd:PRK10433   83 AKFHYYATPAELvPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMAADYPSLNLGQAVMVYCYQ-----LAG 157

                         170
                  ....*....|..
gi 1222367780 164 QEKEIEPKEETA 175
Cdd:PRK10433  158 LMQQPAKSDTTA 169
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-154 5.27e-33

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 116.48  E-value: 5.27e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   4 NIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKP--DSQAIALAAGASDVIgNAQIVDTLDEALAGCSL----VV 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSADpyNPKAIRASAGALFHL-PLVVVDNLEEALKDLKEagyaVV 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1222367780  78 GTSARSrtlpwpmldprecglKSVSEAE-QAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAY 154
Cdd:pfam00588  80 ATSLGG---------------KSLYEIDfPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 6-155 7.29e-28

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 103.46  E-value: 7.29e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   6 RIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALA-----GCsLVVGTs 80
Cdd:cd18082     1 HLVLDNIRSPGNIGSIFRTADAFGVEKIYLVGPTADIFDPKAKRTSRGTHFSVPYERVDNFEEYLKslkeeGY-PLIGL- 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1222367780  81 arsrtlpwpMLDPRECGLKSVSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18082    79 ---------ELTDGSIPLSEFKFKKPKKIALIFGNERSGLSPELLALCDYTVHIPMSGKNSSLNVSVATGIALYE 144
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 16-155 1.63e-08

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 52.04  E-value: 1.63e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  16 GNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAA-GAS---DVIGNAQIVDTLDEALAGCSLVVGTSARSRTLPWpml 91
Cdd:cd18095    12 GNLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASmGALfrlPIIRVDDLEELLEELKEAGYKVVAADLDGGSSLY--- 88

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1222367780  92 dprecglksvSEAEQAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18095    89 ----------DLDLPKPVALVLGNEGNGLSDELLELCDKRVTIPMSGGVESLNVAVAAAILLYE 142
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 3-158 1.23e-07

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 49.77  E-value: 1.23e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   3 QNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLV-NPLVKPDSQAIAlaAGASDVIgNAQIVDTLDEALA-----GCSLV 76
Cdd:cd18092    15 RDLTVVLENVHDPHNASAVLRTCDAFGVQDVHVIePKNKFKKNRGVA--MGADKWL-DVHRHDSTEECLAalkeqGYRIV 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  77 VgTSARSRTLPwpmldPRECGLksvseaeQAPVALVFGRERVGLTNDELQKCHYHVAIaanPEY---SSLNLAMAVQVIA 153
Cdd:cd18092    92 A-TSLSPDAVP-----LDELDY-------TRPTALVFGNEKDGVSEEALAAADERVYI---PMYgfvESLNVSVAAAIIL 155


                  ....*
gi 1222367780 154 YEVRM 158
Cdd:cd18092   156 YELQR 160
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 1-156 1.58e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 46.40  E-value: 1.58e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780   1 MLQNIRivlvetsHTGNMGSVARAMKTMGLTNLWLV-NPLVKPDSQAIALAAGASDVIgNAQIVDTLDEALAGCSlvvgt 79
Cdd:cd18097     4 VLDNLR-------SAHNVGSIFRTADAAGVEKIYLCgYTPTPPHPKVAKTALGAEESV-PWEHFEDTLEAVRALK----- 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  80 sARSRTLpwpmldpreCGLKSVSEA-------EQAPVALVFGRERVGLTNDELQKCHYHVAIaanPEY---SSLNLAMAV 149
Cdd:cd18097    71 -ARGYTI---------IALETTENSvplhearLPRPLALVLGNEVRGVSPEVLALCDAVVEI---PMFgrkNSLNVAVAA 137


                  ....*..
gi 1222367780 150 QVIAYEV 156
Cdd:cd18097   138 AVALYEL 144
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 112-154 9.27e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 44.18  E-value: 9.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1222367780 112 VFGRERVGLTNDELQKCHYHVAIaanPEYSSLNLAMAVQVIAY 154
Cdd:cd18098    97 LLGPEDGGLPQEVLARCHHVVYL---PTRGSLNVAVAGSIVLY 136
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 108-157 9.56e-06

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 45.57  E-value: 9.56e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1222367780 108 PVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVR 157
Cdd:COG0566   210 PVALVLGNEGKGLSRLVLEACDELVRIPMAGGVESLNVAVAAAILLYEAA 259
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 106-158 9.97e-06

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 44.11  E-value: 9.97e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1222367780 106 QAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRM 158
Cdd:cd18104    92 RGPIAIVVGSESEGLSEFWLKAADERVRIPMAGQADSLNVSVAAAILLYEALR 144
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 99-160 1.78e-05

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 43.48  E-value: 1.78e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1222367780  99 KSVSEAE-QAPVALVFGRERVGLTNDELQK-CHYHVAIAANPEYSSLNLAMAVQVIAYEvrmAW 160
Cdd:COG0219    84 KPYTDVDyQPGDVLLFGRETAGLPEEVLAApADQRLRIPMRPGVRSLNLSNAVAIVLYE---AL 144
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 97-156 1.80e-05

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 43.21  E-value: 1.80e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1222367780  97 GLKSVSEAE-QAPVALVFGRERVGLTNDELQKC-HYHVAIAANPEYSSLNLAMAVQVIAYEV 156
Cdd:cd18094    80 GTKPYTDFDfKPGDYLVFGRESTGLPEEILDAFpDRRLRIPMRPKVRSLNLSNSVAIVLYEA 141
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 108-156 2.70e-05

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 42.76  E-value: 2.70e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1222367780 108 PVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEV 156
Cdd:cd18103    95 PLALVLGSEGKGLRRLVLKNCDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 13-156 7.33e-05

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 41.61  E-value: 7.33e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  13 SHTGNMGSVARAMKTMGltnlwlvnplvkpdSQAIALAAGASDVIGNAQIVDTLDEALAgcSLVVGTSARSRTLPW-PML 91
Cdd:cd18107     9 TSPGNLGTIIRSADALG--------------AHGLVVTGHAADVYDPKTIRASTGSLFS--LPIVRVPSNRELEDWvEAR 72

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1222367780  92 DPRECGLKSVSEAEQA-----------PVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEV 156
Cdd:cd18107    73 KARGTPLQLVGTDEHGdhqiadhdftrPTLLLIGNETAGLSAAWRELCDETVSIPMAGSASSLNAACAASIFLYEA 148
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 16-155 4.06e-04

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 39.54  E-value: 4.06e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  16 GNMGSVARAMKTMGLTNLWLVNPLVKP-DSQAIALAAGASDVIGNAQIV--DTLDEALAGCSLVVGTSARSRTLPWPMLD 92
Cdd:cd18106    12 GNLGSILRSAAAAGCSKVLLTKGCVDPwEPKVLRAGAGAHFRLPIYGNLswDDIPNYLPDDSKVFLADNNLPVQPYDSID 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1222367780  93 precglksvseAEQAPVALVFGRERVGLTNDELQKCH----YHVAIAANPEYSSLNLAMAVQVIAYE 155
Cdd:cd18106    92 -----------YCSGPIVLVIGGETHGVSPEAYKLAHstggSRVHIPLANGVDSLNSAVAASILLFE 147
TM1570-like cd18085
SPOUT superfamily RNA methyltransferase TM1570-like; DUF2168; This domain, found in various ...
 21-152 5.08e-04

SPOUT superfamily RNA methyltransferase TM1570-like; DUF2168; This domain, found in various hypothetical prokaryotic proteins, has no known function. It is also found in a few prokaryotic tRNA (guanine-N(1)-)-methyltransferases. Proteins of this family are members of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349958  Cd Length: 178  Bit Score: 39.83  E-value: 5.08e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1222367780  21 VARAMKTMGLTNLWLVNPLvkPDSQAIA--------LAAGAS------DVIGNAQIVDTLDEALAGCS-------LVVGT 79
Cdd:cd18085    30 IARSSRTYGVKGYYIVTPL--EDQQELAerilghwtEGYGGKynpdrkEALSLVRVVESLEEAIAEIEekegkrpLVVAT 107

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1222367780  80 SARSRtlpWPMLDPREcgLKSVSEAEQaPVALVFGRERvGLTNDELQKCHYHVA-IAANPEYSSLNLAMAVQVI 152
Cdd:cd18085   108 SARPY---ANAISYAE--LRRMLLEDR-PVLLVFGTGW-GLAPEVLERADYVLEpIRGAGDYNHLSVRSAAAII 174
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 99-155 4.00e-03

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 36.36  E-value: 4.00e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1222367780  99 KSVSEAE-QAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYS-SLNLAMAVQVIAYE 155
Cdd:cd18109    82 ENLYEFTlPEKGILVMGNEGHGISPEVLKLLTHRLTIPRYGKGAeSLNVAVATAILLSE 140
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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