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Conserved domains on  [gi|1124572624|ref|WP_074855115|]
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MULTISPECIES: lipopolysaccharide heptosyltransferase RfaC [Pseudomonadaceae]

Protein Classification

glycosyltransferase family protein( domain architecture ID 56)

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Glycosyltransferase_GTB-type super family cl10013
glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases ...
1-300 3.91e-167

glycosyltransferase family 1 and related proteins with GTB topology; Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. The structures of the formed glycoconjugates are extremely diverse, reflecting a wide range of biological functions. The members of this family share a common GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


The actual alignment was detected with superfamily member PRK10964:

Pssm-ID: 471961  Cd Length: 322  Bit Score: 467.92  E-value: 3.91e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   1 MRVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHPLRTWRSGEWARFKR 80
Cdd:PRK10964    1 MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWVVEEGFAQIPSWHPAVDRVIPVAIRRWRKAWFSAPIRAERKAFRE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  81 RLRETRYDLVIDAQGLLKSAWL-TRYVSAPVAGLDRDSAREPLASRFYDRRYAVPKDQHALERVRQLFAKALGYTLPSGT 159
Cdd:PRK10964   81 ALQAEQYDAVIDAQGLVKSAALvTRLAHGVKHGMDWQSAREPLASLFYNRRHHIAKQQHAVERTRELFAKSLGYSKPQTQ 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 160 GDYGLNR--TAMMDAAAQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAAVLP 237
Cdd:PRK10964  161 GDYAIAQhfLTNLPADAGPYLVFLHATTRDDKHWPEAHWRELIGLLAPSGLRIKLPWGAEHEEQRAKRLAEGFPYVEVLP 240
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1124572624 238 KLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPN 300
Cdd:PRK10964  241 KLSLEQVARVLAGAKAVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQHACRSPGKS 303
 
Name Accession Description Interval E-value
PRK10964 PRK10964
lipopolysaccharide heptosyltransferase RfaC;
1-300 3.91e-167

lipopolysaccharide heptosyltransferase RfaC;


Pssm-ID: 236809  Cd Length: 322  Bit Score: 467.92  E-value: 3.91e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   1 MRVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHPLRTWRSGEWARFKR 80
Cdd:PRK10964    1 MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWVVEEGFAQIPSWHPAVDRVIPVAIRRWRKAWFSAPIRAERKAFRE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  81 RLRETRYDLVIDAQGLLKSAWL-TRYVSAPVAGLDRDSAREPLASRFYDRRYAVPKDQHALERVRQLFAKALGYTLPSGT 159
Cdd:PRK10964   81 ALQAEQYDAVIDAQGLVKSAALvTRLAHGVKHGMDWQSAREPLASLFYNRRHHIAKQQHAVERTRELFAKSLGYSKPQTQ 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 160 GDYGLNR--TAMMDAAAQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAAVLP 237
Cdd:PRK10964  161 GDYAIAQhfLTNLPADAGPYLVFLHATTRDDKHWPEAHWRELIGLLAPSGLRIKLPWGAEHEEQRAKRLAEGFPYVEVLP 240
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1124572624 238 KLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPN 300
Cdd:PRK10964  241 KLSLEQVARVLAGAKAVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQHACRSPGKS 303
heptsyl_trn_I TIGR02193
lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS ...
2-298 1.50e-144

lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS heptosyltransferase I, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 274025  Cd Length: 319  Bit Score: 410.57  E-value: 1.50e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHP--LRTWRsgEWARFK 79
Cdd:TIGR02193   1 RILIVKTSSLGDVIHTLPALTDIKRALPDVEIDWVVEEGFADIVRLHPAVDEVIPVALRRWRKTLfsAATWR--EIKALR 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  80 RRLRETRYDLVIDAQGLLKSAWLTRYVSAPVAGLDRDSAREPLASRFYDRRYAVPKDQHALERVRQLFAKALGYTLP-SG 158
Cdd:TIGR02193  79 ALLRAERYDAVIDAQGLIKSALVARMARGPRHGFDWRSAREPLASLFYNKRVGISYQQHAVERNRKLFALALGYPPPiAE 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 159 TGDYGLNRTA----MMDAAAQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAA 234
Cdd:TIGR02193 159 TIDYGLARRAavafLGHALPAPYAVLLHATSRDDKTWPEERWRELARLLLARGLQIVLPWGNDAEKQRAERIAEALPGAV 238
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1124572624 235 VLPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASG 298
Cdd:TIGR02193 239 VLPKMSLAEVAALLAGADAVVGVDTGLTHLAAALDKPTVTLYGATDPGRTGGYGKPNVALLGES 302
RfaF COG0859
ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];
1-319 2.55e-88

ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440620  Cd Length: 336  Bit Score: 268.38  E-value: 2.55e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   1 MRVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHplrtwrsGEWARFKR 80
Cdd:COG0859     5 MRILIIRLSALGDVLLATPALRALKRAYPDAEIDLLVEPRFAPLLELNPYVDEVIPFDKKRRKGL-------AELLKLLR 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  81 RLRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDsarepLASRFYDRRYAVPKDQHALERVRQLfAKALGYTLPSGT 159
Cdd:COG0859    78 QLRAERYDLVIDLQGSLRSALLARLAGAPRrIGFDKE-----LRSLLLNHRVPLPPDQHEVERYLAL-LAALGIPLPDPR 151
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 160 GDYGLNRTAMMDAAA---------QPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPwGNETEKARAERIAAGL 230
Cdd:COG0859   152 PDLPLPEEDRAEARAllarlglpgKPYIVLHPGASWPAKRWPAERFAELARALAARGLRVVLL-GGPGERELAEEIAAAL 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 231 THAAV--LPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPNAGSGDR-- 306
Cdd:COG0859   231 GPPVInlAGKTSLRELAALLARADLVVGNDTGPMHLAAALGTPTVALFGPTDPARWGPYGDRHRVLRADLPCSPCGKRec 310
                         330
                  ....*....|....*.
gi 1124572624 307 ---DKPCFDGLGAERV 319
Cdd:COG0859   311 plgHHPCMADISPEEV 326
Glyco_transf_9 pfam01075
Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to ...
75-310 4.02e-80

Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.


Pssm-ID: 395853  Cd Length: 247  Bit Score: 244.16  E-value: 4.02e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  75 WARFKRRLRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDSARepLASRFYDRRYAVPKDQHALERVRQLFAKALGY 153
Cdd:pfam01075   1 RRRLGKALRANAYDRVIDLQGLLKSALLVRFLKAPPrIGFDGESRR--LGSLFYSRKHDKPKGPHAVERNRALFAQALGL 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 154 TLPSGTGDYGLNRTAMMDAAAQ-----PYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNE-TEKARAERIA 227
Cdd:pfam01075  79 PKPESKPELGLSLPFRAAALDAagagrPYIVFCPGASFDAKRWPEEHYAELAEALQERGYQVVLFGGPEaHEEEIAERIA 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 228 AGL--THAAVLPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPNagSGD 305
Cdd:pfam01075 159 AGLeeTCVNLLGKTSLEEAAALLAGADLVVGNDSGLMHLAAALDRPVIGLYGPTDPGRTPPYSDNATIVSLHEGC--SPC 236

                  ....*
gi 1124572624 306 RDKPC 310
Cdd:pfam01075 237 FKKTC 241
GT9_LPS_heptosyltransferase cd03789
lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide ...
2-319 9.55e-58

lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


Pssm-ID: 340821  Cd Length: 277  Bit Score: 187.94  E-value: 9.55e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWrkhplrtWRSGEWARFKRR 81
Cdd:cd03789     1 KILVIRLSALGDVVLTTPLLRALKKAYPDAKITVVVGPGYAELLELNPYIDEVIPDDKRRR-------AGLRGRRKLLRE 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  82 LRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDSAREPLASRFydrryavpkdqhalervrqlfakalgytlpsgtg 160
Cdd:cd03789    74 LRARKYDLVIDLHSSLRSALLLLLSGAPRrIGFDREKRRFLLNHPV---------------------------------- 119
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 161 dyglnrtammdaaaQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAAVLP-KL 239
Cdd:cd03789   120 --------------KPLVVIPPGASGPAKRWPAERFAELADRLADEGYRVVLFGGPAEEELAEEIAAALGARVVNLAgKT 185
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 240 NLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHL------CASGPNAGSGDRDKPCFDG 313
Cdd:cd03789   186 SLRELAALLARADLVVGNDSGPMHLAAALGTPTVALFGPTDPARTGPYGSNHVVVradlpcSPCCPKRECPRGDHKCMRD 265

                  ....*.
gi 1124572624 314 LGAERV 319
Cdd:cd03789   266 ITPEEV 271
 
Name Accession Description Interval E-value
PRK10964 PRK10964
lipopolysaccharide heptosyltransferase RfaC;
1-300 3.91e-167

lipopolysaccharide heptosyltransferase RfaC;


Pssm-ID: 236809  Cd Length: 322  Bit Score: 467.92  E-value: 3.91e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   1 MRVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHPLRTWRSGEWARFKR 80
Cdd:PRK10964    1 MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWVVEEGFAQIPSWHPAVDRVIPVAIRRWRKAWFSAPIRAERKAFRE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  81 RLRETRYDLVIDAQGLLKSAWL-TRYVSAPVAGLDRDSAREPLASRFYDRRYAVPKDQHALERVRQLFAKALGYTLPSGT 159
Cdd:PRK10964   81 ALQAEQYDAVIDAQGLVKSAALvTRLAHGVKHGMDWQSAREPLASLFYNRRHHIAKQQHAVERTRELFAKSLGYSKPQTQ 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 160 GDYGLNR--TAMMDAAAQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAAVLP 237
Cdd:PRK10964  161 GDYAIAQhfLTNLPADAGPYLVFLHATTRDDKHWPEAHWRELIGLLAPSGLRIKLPWGAEHEEQRAKRLAEGFPYVEVLP 240
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1124572624 238 KLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPN 300
Cdd:PRK10964  241 KLSLEQVARVLAGAKAVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQHACRSPGKS 303
heptsyl_trn_I TIGR02193
lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS ...
2-298 1.50e-144

lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS heptosyltransferase I, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 274025  Cd Length: 319  Bit Score: 410.57  E-value: 1.50e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHP--LRTWRsgEWARFK 79
Cdd:TIGR02193   1 RILIVKTSSLGDVIHTLPALTDIKRALPDVEIDWVVEEGFADIVRLHPAVDEVIPVALRRWRKTLfsAATWR--EIKALR 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  80 RRLRETRYDLVIDAQGLLKSAWLTRYVSAPVAGLDRDSAREPLASRFYDRRYAVPKDQHALERVRQLFAKALGYTLP-SG 158
Cdd:TIGR02193  79 ALLRAERYDAVIDAQGLIKSALVARMARGPRHGFDWRSAREPLASLFYNKRVGISYQQHAVERNRKLFALALGYPPPiAE 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 159 TGDYGLNRTA----MMDAAAQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAA 234
Cdd:TIGR02193 159 TIDYGLARRAavafLGHALPAPYAVLLHATSRDDKTWPEERWRELARLLLARGLQIVLPWGNDAEKQRAERIAEALPGAV 238
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1124572624 235 VLPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASG 298
Cdd:TIGR02193 239 VLPKMSLAEVAALLAGADAVVGVDTGLTHLAAALDKPTVTLYGATDPGRTGGYGKPNVALLGES 302
RfaF COG0859
ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];
1-319 2.55e-88

ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440620  Cd Length: 336  Bit Score: 268.38  E-value: 2.55e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   1 MRVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWRKHplrtwrsGEWARFKR 80
Cdd:COG0859     5 MRILIIRLSALGDVLLATPALRALKRAYPDAEIDLLVEPRFAPLLELNPYVDEVIPFDKKRRKGL-------AELLKLLR 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  81 RLRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDsarepLASRFYDRRYAVPKDQHALERVRQLfAKALGYTLPSGT 159
Cdd:COG0859    78 QLRAERYDLVIDLQGSLRSALLARLAGAPRrIGFDKE-----LRSLLLNHRVPLPPDQHEVERYLAL-LAALGIPLPDPR 151
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 160 GDYGLNRTAMMDAAA---------QPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPwGNETEKARAERIAAGL 230
Cdd:COG0859   152 PDLPLPEEDRAEARAllarlglpgKPYIVLHPGASWPAKRWPAERFAELARALAARGLRVVLL-GGPGERELAEEIAAAL 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 231 THAAV--LPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPNAGSGDR-- 306
Cdd:COG0859   231 GPPVInlAGKTSLRELAALLARADLVVGNDTGPMHLAAALGTPTVALFGPTDPARWGPYGDRHRVLRADLPCSPCGKRec 310
                         330
                  ....*....|....*.
gi 1124572624 307 ---DKPCFDGLGAERV 319
Cdd:COG0859   311 plgHHPCMADISPEEV 326
Glyco_transf_9 pfam01075
Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to ...
75-310 4.02e-80

Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.


Pssm-ID: 395853  Cd Length: 247  Bit Score: 244.16  E-value: 4.02e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  75 WARFKRRLRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDSARepLASRFYDRRYAVPKDQHALERVRQLFAKALGY 153
Cdd:pfam01075   1 RRRLGKALRANAYDRVIDLQGLLKSALLVRFLKAPPrIGFDGESRR--LGSLFYSRKHDKPKGPHAVERNRALFAQALGL 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 154 TLPSGTGDYGLNRTAMMDAAAQ-----PYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNE-TEKARAERIA 227
Cdd:pfam01075  79 PKPESKPELGLSLPFRAAALDAagagrPYIVFCPGASFDAKRWPEEHYAELAEALQERGYQVVLFGGPEaHEEEIAERIA 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 228 AGL--THAAVLPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHLCASGPNagSGD 305
Cdd:pfam01075 159 AGLeeTCVNLLGKTSLEEAAALLAGADLVVGNDSGLMHLAAALDRPVIGLYGPTDPGRTPPYSDNATIVSLHEGC--SPC 236

                  ....*
gi 1124572624 306 RDKPC 310
Cdd:pfam01075 237 FKKTC 241
GT9_LPS_heptosyltransferase cd03789
lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide ...
2-319 9.55e-58

lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


Pssm-ID: 340821  Cd Length: 277  Bit Score: 187.94  E-value: 9.55e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIRRWrkhplrtWRSGEWARFKRR 81
Cdd:cd03789     1 KILVIRLSALGDVVLTTPLLRALKKAYPDAKITVVVGPGYAELLELNPYIDEVIPDDKRRR-------AGLRGRRKLLRE 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  82 LRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDSAREPLASRFydrryavpkdqhalervrqlfakalgytlpsgtg 160
Cdd:cd03789    74 LRARKYDLVIDLHSSLRSALLLLLSGAPRrIGFDREKRRFLLNHPV---------------------------------- 119
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 161 dyglnrtammdaaaQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWGNETEKARAERIAAGLTHAAVLP-KL 239
Cdd:cd03789   120 --------------KPLVVIPPGASGPAKRWPAERFAELADRLADEGYRVVLFGGPAEEELAEEIAAALGARVVNLAgKT 185
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 240 NLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPGKVGAYGRSQIHL------CASGPNAGSGDRDKPCFDG 313
Cdd:cd03789   186 SLRELAALLARADLVVGNDSGPMHLAAALGTPTVALFGPTDPARTGPYGSNHVVVradlpcSPCCPKRECPRGDHKCMRD 265

                  ....*.
gi 1124572624 314 LGAERV 319
Cdd:cd03789   266 ITPEEV 271
heptsyl_trn_III TIGR02201
lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the ...
2-279 1.09e-17

lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the putative ADP-heptose:LPS heptosyltransferase III, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. This enzyme may be less widely distributed than heptosyltransferases I and II. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 131256  Cd Length: 344  Bit Score: 82.65  E-value: 1.09e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAiRRWRKHPLRTWRSgEWaRFKRR 81
Cdd:TIGR02201   1 RILLIKLRHHGDMLLTTPVISSLKKNYPDAKIDVLLYQETIPILSENPDINALYGLD-RKKAKAGERKLAN-QF-HLIKV 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  82 LRETRYDLVIDAQGLLKSAWLTRYVSAPVA-GLDRDSAREPLASRFYDrrYAVPKDQH-ALERVRQ--LFAKALGY---- 153
Cdd:TIGR02201  78 LRANRYDLVVNLTDQWMVAILVKLLNARVKiGFDYPKRRSAFWRKSFT--ALAPLQGGnTLHTVEQnlSVLTPLGLdslv 155
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 154 ---TLPSGTGDYGLNRTAMMDAA-AQPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWG-NETEKARAERIAA 228
Cdd:TIGR02201 156 kqtRMSYPPADWKAMRALLDEAGvGQNYIVIQPTSRWFFKCWDNDRFSALIDALHARGYEVVLTSGpDKDELAMVNEIAQ 235
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1124572624 229 GLTHA---AVLPKLNLAGVAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPT 279
Cdd:TIGR02201 236 GCQTPrvtSLAGKLTLPQLAALIDHARLFIGVDSVPMHMAAALGTPLVALFGPS 289
heptsyl_trn_II TIGR02195
lipopolysaccharide heptosyltransferase II; This family consists of examples of ADP-heptose:LPS ...
2-282 6.06e-17

lipopolysaccharide heptosyltransferase II; This family consists of examples of ADP-heptose:LPS heptosyltransferase II, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 274026  Cd Length: 334  Bit Score: 80.11  E-value: 6.06e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVV--HTLPALTDAARaiPGIRFDWVVEEGFAEIPAWHPAVSQVIPVAIrrwrKHplRTWRSGEWARFK 79
Cdd:TIGR02195   1 KILVIGPSWVGDMVmaQSLYRLLKKRY--PQAVIDVLAPAWCRPLLERMPEIRQAIDMPL----GH--GALELTERRRLG 72
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  80 RRLRETRYDLVIDAQGLLKSAWLTRYVSAPV-AGLDRDSareplasrfydrRYAVPKDQHALERVR-----QLFAkALGY 153
Cdd:TIGR02195  73 RSLREERYDQAIVLPNSLKSALIPFFAGIPHrTGWRGEM------------RYGLLNDVRALDKERlplmvERYI-ALAY 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 154 T----LPS-----------GTGDYGLNRTAMmdAAAQPYLVFLHGTTWA-SKHWPEADWRALAERMDELGWAVRLpWGNE 217
Cdd:TIGR02195 140 DkgqdLPQplprpqlqvspAEQAAALAKFGL--DTERPIIAFCPGAEFGpAKRWPHEHYAELAKRLIDQGYQVVL-FGSA 216
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1124572624 218 TEKARAERIAAGLTHAAVlpklNLAGVAK------VIAGASACVSVDTGLGHLAAALDVPNISLYGPTLPG 282
Cdd:TIGR02195 217 KDHPAGNEIEALLPGELR----NLAGETSldeavdLIALAKAVVTNDSGLMHVAAALNRPLVALYGSTSPD 283
PRK10916 PRK10916
ADP-heptose--LPS heptosyltransferase RfaF;
188-281 2.24e-11

ADP-heptose--LPS heptosyltransferase RfaF;


Pssm-ID: 182835  Cd Length: 348  Bit Score: 63.92  E-value: 2.24e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 188 SKHWPEADWRALAERMDELGWAVRLpWGNETEKARAERIAAGLTHAAVLPKLNLAGVAK------VIAGASACVSVDTGL 261
Cdd:PRK10916  194 AKRWPHYHYAELAQQLIDEGYQVVL-FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQleqaviLIAACKAIVTNDSGL 272
                          90       100
                  ....*....|....*....|
gi 1124572624 262 GHLAAALDVPNISLYGPTLP 281
Cdd:PRK10916  273 MHVAAALNRPLVALYGPSSP 292
PRK10422 PRK10422
lipopolysaccharide core biosynthesis protein; Provisional
2-279 8.53e-07

lipopolysaccharide core biosynthesis protein; Provisional


Pssm-ID: 182447  Cd Length: 352  Bit Score: 50.16  E-value: 8.53e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624   2 RVLIIKTSSLGDVVHTLPALTDAARAIPGIRFDWVVEEGFAEIPAWHPAVSQVipVAIRRWRKHPLRTWRSgeWARFKRR 81
Cdd:PRK10422    7 RILIIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPILSENPEINAL--YGIKNKKAGASEKIKN--FFSLIKV 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624  82 LRETRYDLVIDAQGLLKSAWLTRYVSA-PVAGLDRDSAREPLASRFYDrrYAVP-KDQHALERVRQLFaKALGY------ 153
Cdd:PRK10422   83 LRANKYDLIVNLTDQWMVALLVRLLNArVKISQDYHHRQSAFWRKSFT--HLVPlQGGHIVESNLSVL-TPLGLsslvke 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1124572624 154 -TLPSGTGDYGLNRTAMmDAAA--QPYLVFLHGTTWASKHWPEADWRALAERMDELGWAVRLPWG-NETEKARAERIAAG 229
Cdd:PRK10422  160 tTMSYRPESWKRMRRQL-DHLGvtQNYVVIQPTARQIFKCWDNDKFSAVIDALQARGYEVVLTSGpDKDDLACVNEIAQG 238
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1124572624 230 LTHAavlPKLNLAG------VAKVIAGASACVSVDTGLGHLAAALDVPNISLYGPT 279
Cdd:PRK10422  239 CQTP---PVTALAGkttfpeLGALIDHAQLFIGVDSAPAHIAAAVNTPLICLFGAT 291
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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