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Conserved domains on  [gi|1046480918|ref|WP_065651205|]
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MULTISPECIES: GPW/gp25 family protein [Gilliamella]

Protein Classification

GPW/gp25 family protein( domain architecture ID 10007624)

GPW/gp25 family protein similar to type VI secretion system (T6SS) baseplate subunit TssE, which is a component of the T6SS baseplate that serves not only as the tail assembly platform, but also docks the tail to the membrane complex

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
1-112 6.69e-38

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


:

Pssm-ID: 442846  Cd Length: 119  Bit Score: 123.83  E-value: 6.69e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918   1 MSYIGMNCKTGRT--INDMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKL 78
Cdd:COG3628     1 MSEPGFDRATGRVamVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEV 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1046480918  79 DAVDVFPDKE---KLTLQITGSRIDkPNQTFTSEIEV 112
Cdd:COG3628    81 ESVDVTPDPEedgKLLITIDYTVRA-TNQRFNLVYPF 116
 
Name Accession Description Interval E-value
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
1-112 6.69e-38

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 123.83  E-value: 6.69e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918   1 MSYIGMNCKTGRT--INDMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKL 78
Cdd:COG3628     1 MSEPGFDRATGRVamVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEV 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1046480918  79 DAVDVFPDKE---KLTLQITGSRIDkPNQTFTSEIEV 112
Cdd:COG3628    81 ESVDVTPDPEedgKLLITIDYTVRA-TNQRFNLVYPF 116
W PHA02516
baseplate wedge subunit; Provisional
 6-101 1.05e-35

baseplate wedge subunit; Provisional


Pssm-ID: 134016  Cd Length: 103  Bit Score: 117.88  E-value: 1.05e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918   6 MNCKTGRTINDMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKLDAVDV-F 84
Cdd:PHA02516    1 MNRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGSLLPDLIDQPQNPALRLQIYAACAMALMRWEPRITLTRVQIeR 80

                          90
                  ....*....|....*..
gi 1046480918  85 PDKEKLTLQITGSRIDK 101
Cdd:PHA02516   81 AADGRMTVDITGWHVDT 97
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 16-102 6.10e-16

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 67.22  E-value: 6.10e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918  16 DMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKLDAVDVFPDKE---KLTL 92
Cdd:pfam04965   3 LRESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEVDPDPDspgTLRF 82

                          90
                  ....*....|
gi 1046480918  93 QITGSRIDKP 102
Cdd:pfam04965  83 TIEYTLRATP 92
 
Name Accession Description Interval E-value
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
1-112 6.69e-38

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 123.83  E-value: 6.69e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918   1 MSYIGMNCKTGRT--INDMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKL 78
Cdd:COG3628     1 MSEPGFDRATGRVamVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEV 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1046480918  79 DAVDVFPDKE---KLTLQITGSRIDkPNQTFTSEIEV 112
Cdd:COG3628    81 ESVDVTPDPEedgKLLITIDYTVRA-TNQRFNLVYPF 116
W PHA02516
baseplate wedge subunit; Provisional
 6-101 1.05e-35

baseplate wedge subunit; Provisional


Pssm-ID: 134016  Cd Length: 103  Bit Score: 117.88  E-value: 1.05e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918   6 MNCKTGRTINDMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKLDAVDV-F 84
Cdd:PHA02516    1 MNRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGSLLPDLIDQPQNPALRLQIYAACAMALMRWEPRITLTRVQIeR 80

                          90
                  ....*....|....*..
gi 1046480918  85 PDKEKLTLQITGSRIDK 101
Cdd:PHA02516   81 AADGRMTVDITGWHVDT 97
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 16-102 6.10e-16

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 67.22  E-value: 6.10e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918  16 DMDHINQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKLDAVDVFPDKE---KLTL 92
Cdd:pfam04965   3 LRESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEVDPDPDspgTLRF 82

                          90
                  ....*....|
gi 1046480918  93 QITGSRIDKP 102
Cdd:pfam04965  83 TIEYTLRATP 92
25 PHA00415
baseplate wedge subunit
 20-113 3.17e-06

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 43.07  E-value: 3.17e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1046480918  20 INQSIKDILTTPIGSRIERRSYGSLLFLLLDNPNTEATKLRVISATVLALAQWEPRIKLDAVDVFP--DKEKLTLQITGS 97
Cdd:PHA00415   33 IKNSLLGIVTTRKGERPFDPNFGCDISDQLFENMDPLTADTIERNIESAIRNYEPRVYNLNVEVIPvyDDNSIIVTIYFS 112

                          90
                  ....*....|....*.
gi 1046480918  98 RIDKPNQTFTSEIEVA 113
Cdd:PHA00415  113 IIDNPDDLEQIKFQLS 128
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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