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Conserved domains on  [gi|1028642438|ref|WP_063989066|]
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MULTISPECIES: GNAT family N-acetyltransferase [Serratia]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-144 8.72e-11

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 56.54  E-value: 8.72e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   2 TPIVRTAT---LEEIHRLYQRIPEFGG------LHSLADLQRRIGAAPAS---LLIAEIGGQPAGFKLGYQRQETVFYSW 69
Cdd:COG1247     1 EMTIRPATpedAPAIAAIYNEAIAEGTatfetePPSEEEREAWFAAILAPgrpVLVAEEDGEVVGFASLGPFRPRPAYRG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  70 LG----GVLPTCRRHGVAQALLAEQERWARAQGYRQLTVKTRNRFRAMLTMLLAHHYQIV-QLEKIGEVADYRL---LLE 141
Cdd:COG1247    81 TAeesiYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVgTLPEVGFKFGRWLdlvLMQ 160


                  ...
gi 1028642438 142 KNL 144
Cdd:COG1247   161 KRL 163
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-144 8.72e-11

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 56.54  E-value: 8.72e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   2 TPIVRTAT---LEEIHRLYQRIPEFGG------LHSLADLQRRIGAAPAS---LLIAEIGGQPAGFKLGYQRQETVFYSW 69
Cdd:COG1247     1 EMTIRPATpedAPAIAAIYNEAIAEGTatfetePPSEEEREAWFAAILAPgrpVLVAEEDGEVVGFASLGPFRPRPAYRG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  70 LG----GVLPTCRRHGVAQALLAEQERWARAQGYRQLTVKTRNRFRAMLTMLLAHHYQIV-QLEKIGEVADYRL---LLE 141
Cdd:COG1247    81 TAeesiYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVgTLPEVGFKFGRWLdlvLMQ 160


                  ...
gi 1028642438 142 KNL 144
Cdd:COG1247   161 KRL 163
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 10-108 1.96e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.44  E-value: 1.96e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  10 LEEIHRLYQRIPEFGGLHSLADLQR-RIGAAPASLLIAEIGGQPAGFKLGYQRQETVFYSWLGG--VLPTCRRHGVAQAL 86
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEdWDEDASEGFFVAEEDGELVGFASLSIIDDEPPVGEIEGlaVAPEYRGKGIGTAL 80

                          90       100
                  ....*....|....*....|..
gi 1028642438  87 LAEQERWARAQGYRQLTVKTRN 108
Cdd:pfam00583  81 LQALLEWARERGCERIFLEVAA 102
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 43-104 1.18e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.11  E-value: 1.18e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1028642438  43 LLIAEIGGQPAGFKLGYQRQETVFYSWLG--GVLPTCRRHGVAQALLAEQERWARAQGYRQLTV 104
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSGGDTAYIGdlAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
PRK03624 PRK03624
putative acetyltransferase; Provisional
 30-108 2.88e-04

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 38.37  E-value: 2.88e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  30 ADLQRRIGAAPASLLIAEIGGQPAGFKL-GYQRQETVFYSWlgGVLPTCRRHGVAQALLAEQERWARAQGYR--QLTVKT 106
Cdd:PRK03624   34 MDIERKLNHDPSLFLVAEVGGEVVGTVMgGYDGHRGWAYYL--AVHPDFRGRGIGRALVARLEKKLIARGCPkiNLQVRE 111


                  ..
gi 1028642438 107 RN 108
Cdd:PRK03624  112 DN 113
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-144 8.72e-11

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 56.54  E-value: 8.72e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   2 TPIVRTAT---LEEIHRLYQRIPEFGG------LHSLADLQRRIGAAPAS---LLIAEIGGQPAGFKLGYQRQETVFYSW 69
Cdd:COG1247     1 EMTIRPATpedAPAIAAIYNEAIAEGTatfetePPSEEEREAWFAAILAPgrpVLVAEEDGEVVGFASLGPFRPRPAYRG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  70 LG----GVLPTCRRHGVAQALLAEQERWARAQGYRQLTVKTRNRFRAMLTMLLAHHYQIV-QLEKIGEVADYRL---LLE 141
Cdd:COG1247    81 TAeesiYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVgTLPEVGFKFGRWLdlvLMQ 160


                  ...
gi 1028642438 142 KNL 144
Cdd:COG1247   161 KRL 163
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 10-108 1.96e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.44  E-value: 1.96e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  10 LEEIHRLYQRIPEFGGLHSLADLQR-RIGAAPASLLIAEIGGQPAGFKLGYQRQETVFYSWLGG--VLPTCRRHGVAQAL 86
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEdWDEDASEGFFVAEEDGELVGFASLSIIDDEPPVGEIEGlaVAPEYRGKGIGTAL 80

                          90       100
                  ....*....|....*....|..
gi 1028642438  87 LAEQERWARAQGYRQLTVKTRN 108
Cdd:pfam00583  81 LQALLEWARERGCERIFLEVAA 102
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
5-112 9.62e-08

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 48.16  E-value: 9.62e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   5 VRTAT---LEEIHRLYQRipEFGGlHSLADLQRRIGAAPAS--LLIAEIGGQPAGF----KLGYQRQETVFYSWLGGVLP 75
Cdd:COG3153     1 IRPATpedAEAIAALLRA--AFGP-GREAELVDRLREDPAAglSLVAEDDGEIVGHvalsPVDIDGEGPALLLGPLAVDP 77

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1028642438  76 TCRRHGVAQALLAEQERWARAQGYRQLTVKTRNRFRA 112
Cdd:COG3153    78 EYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLP 114
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 43-104 1.18e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 46.11  E-value: 1.18e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1028642438  43 LLIAEIGGQPAGFKLGYQRQETVFYSWLG--GVLPTCRRHGVAQALLAEQERWARAQGYRQLTV 104
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSGGDTAYIGdlAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 4-109 1.50e-07

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 47.36  E-value: 1.50e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   4 IVRTATLEEIHRLYQRIPefgglhSLADLQRRIGA-APASLLIAEIGGQPAGFKLGYQRQETVFYSWLGGVLPTCRRHGV 82
Cdd:COG0454     2 SIRKATPEDINFILLIEA------LDAELKAMEGSlAGAEFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVLPEYRGKGI 75

                          90       100
                  ....*....|....*....|....*..
gi 1028642438  83 AQALLAEQERWARAQGYRQLTVKTRNR 109
Cdd:COG0454    76 GKALLEALLEWARERGCTALELDTLDG 102
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 4-107 1.80e-07

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 46.91  E-value: 1.80e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438   4 IVRTATLEEIHRLYQripefggLHSLADLQRRIGAapasLLIAEIGGQPAGFKLGYQRQETVFYSWLGGVLPTCRRHGVA 83
Cdd:COG1246     2 TIRPATPDDVPAILE-------LIRPYALEEEIGE----FWVAEEDGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIG 70

                          90       100
                  ....*....|....*....|....
gi 1028642438  84 QALLAEQERWARAQGYRQLTVKTR 107
Cdd:COG1246    71 RRLLEALLAEARELGLKRLFLLTT 94
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 41-109 4.06e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 42.44  E-value: 4.06e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  41 ASLLIAEIGGQPAGFKLGYQRQETVFYSWLG-GVLPTCRRHGVAQALLAEQERWARAQGYRQLTVKTRNR 109
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPLDDEGALAELRlAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNR 72
COG3375 COG3375
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
19-103 1.22e-05

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 442602 [Multi-domain]  Cd Length: 271  Bit Score: 43.40  E-value: 1.22e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  19 RIPEFGGLHSLADLQRRI-GAAPAS----------------LLIAEIGGQPAGFK---LGYQRQETVFYSWLGGVLPTCR 78
Cdd:COG3375     9 ELTTPAELEAVQRLQRSVwGSPPNEpvpvhllralahaggyVLGAFDGGELVGFSfgfPGFRPGGLYLHSHMAGVLPEYR 88

                          90       100
                  ....*....|....*....|....*
gi 1028642438  79 RHGVAQALLAEQERWARAQGYRQLT 103
Cdd:COG3375    89 GRGVGYALKLHQREWALERGVTLIT 113
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 72-144 8.01e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 39.25  E-value: 8.01e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1028642438  72 GVLPTCRRHGVAQALLAEQERWARAQGYRQLTVKTRNRFRAMLTMLLAHHYQIVQLEKiGEVADYRLLLEKNL 144
Cdd:COG0456    20 AVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERP-NYYGDDALVMEKEL 91
PRK03624 PRK03624
putative acetyltransferase; Provisional
 30-108 2.88e-04

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 38.37  E-value: 2.88e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  30 ADLQRRIGAAPASLLIAEIGGQPAGFKL-GYQRQETVFYSWlgGVLPTCRRHGVAQALLAEQERWARAQGYR--QLTVKT 106
Cdd:PRK03624   34 MDIERKLNHDPSLFLVAEVGGEVVGTVMgGYDGHRGWAYYL--AVHPDFRGRGIGRALVARLEKKLIARGCPkiNLQVRE 111


                  ..
gi 1028642438 107 RN 108
Cdd:PRK03624  112 DN 113
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
46-100 4.58e-04

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 37.06  E-value: 4.58e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1028642438  46 AEIGGQPAGFkLGYQRQETVFYSWLGGVLPTCRRHGVAQALLAEQERWARAQGYR 100
Cdd:COG2388    14 LEVDGELAGE-LTYRLEGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLK 67
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
67-108 1.09e-03

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 36.04  E-value: 1.09e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1028642438  67 YSWLGGV--LPTCRRHGVAQALLAEQERWARAQGYRQ--LTVKTRN 108
Cdd:COG3393    15 VAEISGVytHPEYRGRGLASALVAALAREALARGARTpfLYVDADN 60
Acetyltransf_6 pfam13480
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 41-100 1.53e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions.


Pssm-ID: 433244 [Multi-domain]  Cd Length: 143  Bit Score: 36.48  E-value: 1.53e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1028642438  41 ASLLIAEIGGQPAGFKLGYqRQETVFYSWLGGVLPTCRRHGVAQALLAEQERWARAQGYR 100
Cdd:pfam13480  72 LRLYVLRLDGRPVAALLGL-RSGDRLYYWFGGYDPEYARLSPGLLLLWELIEHAAEEGLR 130
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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