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Conserved domains on  [gi|939351490|ref|WP_054896818|]
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MULTISPECIES: delta-9 fatty acid desaturase DesA [Pseudomonas]

Protein Classification

acyl-CoA desaturase( domain architecture ID 10787619)

acyl-CoA desaturase removes two hydrogen atoms from a fatty acid, creating a carbon/carbon double bond; similar to acyl-CoA delta(9) desaturase and acyl-CoA delta(11) desaturase; may also contain a transposase domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Delta9-FADS-like cd03505
The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl ...
 12-242 1.55e-47

The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl CoA desaturases found in various eukaryotes including vertebrates, insects, higher plants, and fungi. The delta-9 acyl-lipid desaturases are found in a wide range of bacteria. These enzymes play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. Acyl-CoA desaturases are the enzymes involved in the CoA-bound desaturation of fatty acids. Mammalian stearoyl-CoA delta-9 desaturase is a key enzyme in the biosynthesis of monounsaturated fatty acids, and in yeast, the delta-9 acyl-CoA desaturase (OLE1) reaction accounts for all de nova unsaturated fatty acid production in Saccharomyces cerevisiae. These non-heme, iron-containing, ER membrane-bound enzymes are part of a three-component enzyme system involving cytochrome b5, cytochrome b5 reductase, and the delta-9 fatty acid desaturase. This complex catalyzes the NADH- and oxygen-dependent insertion of a cis double bond between carbons 9 and 10 of the saturated fatty acyl substrates, palmitoyl (16:0)-CoA or stearoyl (18:0)-CoA, yielding the monoenoic products palmitoleic (16:l) or oleic (18:l) acids, respectively. In cyanobacteria, the biosynthesis of unsaturated fatty acids is initiated by delta 9 acyl-lipid desaturase (DesC) which introduces the first double bond at the delta-9 position of a saturated fatty acid that has been esterified to a glycerolipid. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXXH, HXXHH, and H/QXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase. Some eukaryotic (Fungi, Euglenozoa, Mycetozoa, Rhodophyta) desaturase domains have an adjacent C-terminal cytochrome b5-like domain.


:

Pssm-ID: 239582  Cd Length: 178  Bit Score: 160.03  E-value: 1.55e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPvikg 91
Cdd:cd03505    2 WATLVFLVLYYLLTGLGITAGYHRLWAHRSFKAPKPLRIFLAILGSLAGQGSPLWWVADHRLHHRYSDTDGDPHSP---- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  92 lstvlRKGaelyraeaenpetlriygkncpddwiernlytrypllgvalmgvidlLLFGTIGITIWAIQMMWIPFWAAGV 171
Cdd:cd03505   78 -----KRG-----------------------------------------------FWFSHVGWLGGLLRIVLVLHATWLV 105

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939351490 172 iNGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIKVFSFLRLAK 242
Cdd:cd03505  106 -NSLAHMWGYRPYDTRDTSRNNWWVALLTFGEGWHNNHHAFPGDARNGLKWYQIDPTKWVIRLLEKLGLAW 175
DDE_Tnp_ISL3 super family cl47205
Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains ...
 271-387 5.42e-04

Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains transposases for IS204, IS1001, IS1096 and IS1165.


The actual alignment was detected with superfamily member pfam01610:

Pssm-ID: 426344  Cd Length: 238  Bit Score: 41.04  E-value: 5.42e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  271 RFQIMAqyrklvigpLVKQELEKVDHSVRHQFH--------RAKRLLSRETSLLEDRHHLRIQSMLEHSHALTVIYEKRL 342
Cdd:pfam01610  81 RFHVVK---------LANEALDEVRRREQKELKerlrkglkGSRWLLLKNPENLTEEQKQRLDELLDLSPKLARAYLLKE 151

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 939351490  343 ALQQIWLKTSSngHDMLAAIKEWVHEAEASGIQSLRDFAHQLKTY 387
Cdd:pfam01610 152 ALRQIWRYADR--AEARKFLDEWINWARRSGLPPFKKLAKTLRNH 194
 
Name Accession Description Interval E-value
Delta9-FADS-like cd03505
The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl ...
 12-242 1.55e-47

The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl CoA desaturases found in various eukaryotes including vertebrates, insects, higher plants, and fungi. The delta-9 acyl-lipid desaturases are found in a wide range of bacteria. These enzymes play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. Acyl-CoA desaturases are the enzymes involved in the CoA-bound desaturation of fatty acids. Mammalian stearoyl-CoA delta-9 desaturase is a key enzyme in the biosynthesis of monounsaturated fatty acids, and in yeast, the delta-9 acyl-CoA desaturase (OLE1) reaction accounts for all de nova unsaturated fatty acid production in Saccharomyces cerevisiae. These non-heme, iron-containing, ER membrane-bound enzymes are part of a three-component enzyme system involving cytochrome b5, cytochrome b5 reductase, and the delta-9 fatty acid desaturase. This complex catalyzes the NADH- and oxygen-dependent insertion of a cis double bond between carbons 9 and 10 of the saturated fatty acyl substrates, palmitoyl (16:0)-CoA or stearoyl (18:0)-CoA, yielding the monoenoic products palmitoleic (16:l) or oleic (18:l) acids, respectively. In cyanobacteria, the biosynthesis of unsaturated fatty acids is initiated by delta 9 acyl-lipid desaturase (DesC) which introduces the first double bond at the delta-9 position of a saturated fatty acid that has been esterified to a glycerolipid. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXXH, HXXHH, and H/QXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase. Some eukaryotic (Fungi, Euglenozoa, Mycetozoa, Rhodophyta) desaturase domains have an adjacent C-terminal cytochrome b5-like domain.


Pssm-ID: 239582  Cd Length: 178  Bit Score: 160.03  E-value: 1.55e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPvikg 91
Cdd:cd03505    2 WATLVFLVLYYLLTGLGITAGYHRLWAHRSFKAPKPLRIFLAILGSLAGQGSPLWWVADHRLHHRYSDTDGDPHSP---- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  92 lstvlRKGaelyraeaenpetlriygkncpddwiernlytrypllgvalmgvidlLLFGTIGITIWAIQMMWIPFWAAGV 171
Cdd:cd03505   78 -----KRG-----------------------------------------------FWFSHVGWLGGLLRIVLVLHATWLV 105

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939351490 172 iNGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIKVFSFLRLAK 242
Cdd:cd03505  106 -NSLAHMWGYRPYDTRDTSRNNWWVALLTFGEGWHNNHHAFPGDARNGLKWYQIDPTKWVIRLLEKLGLAW 175
OLE1 COG1398
Fatty-acid desaturase [Lipid transport and metabolism];
12-252 2.32e-36

Fatty-acid desaturase [Lipid transport and metabolism];


Pssm-ID: 441008  Cd Length: 286  Bit Score: 133.80  E-value: 2.32e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFrFWLWLTTAQNTR-EWTAIHRKHHAKCETVDDPHSPVIK 90
Cdd:COG1398   43 WSAVALALVLYVLTGLGITVGYHRLFSHRSFKTPRWLEYLL-AILGALALQGGPlWWVADHRRHHRHSDTEGDPHSPKLR 121

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  91 GL--STVlrkGAELYRAEAENPEtlriygKNCPDD-------WIERNLYTRYPLLGVALMGVIDLLLFGTIGITIWAI-- 159
Cdd:COG1398  122 GFwwSHM---GWMLREDPTPNDY------RYAPDLakdpelrWLDRYYLLLQLALGLLLPALLGGLLGGGWSGLLWGGfv 192

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490 160 ------QMMWipfwaagVINGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIK 233
Cdd:COG1398  193 rtvllhHGTW-------FINSLAHVWGYRPFETRDTSRNNWWLALLTFGEGWHNNHHAFPTSARHGLRWWEIDPTWWLIR 265

                        250       260
                 ....*....|....*....|
gi 939351490 234 VFSFLRLA-KVQRVAPIAHR 252
Cdd:COG1398  266 LLEKLGLAwDVRRPPAERIA 285
PLN02220 PLN02220
delta-9 acyl-lipid desaturase
 12-241 1.84e-16

delta-9 acyl-lipid desaturase


Pssm-ID: 177866 [Multi-domain]  Cd Length: 299  Bit Score: 79.08  E-value: 1.84e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPvIKG 91
Cdd:PLN02220  55 WEALRFGLILYIVTGLSITFSYHRNLAHRSFKLPKWLEYPFAYSALFALQGDPIDWVSTHRFHHQFTDSDRDPHSP-IEG 133

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  92 L--STVLrkgaelyraeaenpetlriygkncpddWIERNLYTRYPLLGVAlmGVIDL-------LLFGTIGITI---WAI 159
Cdd:PLN02220 134 FwfSHVL---------------------------WIFDTSYIREKCGGRD--NVMDLkqqwfyrFLRKTIGLHIlmfWTL 184

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490 160 QMMW--IPF--WAAGV-----------INGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWE 224
Cdd:PLN02220 185 LYLWggLPYltWGVGVggaigyhvtwlINSACHIWGSRTWKTKDTSRNVWWLSLFTMGESWHNNHHAFESSARQGLEWWQ 264

                        250
                 ....*....|....*..
gi 939351490 225 FDLGWAWIKVFSFLRLA 241
Cdd:PLN02220 265 IDITWYLIRFFEVLGLA 281
FA_desaturase pfam00487
Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a ...
 11-214 8.76e-06

Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a double bond at the delta position of fatty acids. There seem to be two distinct families of fatty acid desaturases which do not seem to be evolutionary related: Family 1 composed of Stearoyl-CoA desaturases (SCD) and Family 2 composed of Bacterial fatty acid desaturases, Plant stearoyl-acyl-carrier-protein desaturase and Cyanobacterial DesA. Members of this entry are ER integral membrane proteins that share the same mushroom-shaped fold consisting of four transmembrane helices (TM1-TM4) which anchor them to the membrane, capped by a cytosolic domain containing a unique 9-10 histidine- coordinating di metal (di-iron) catalytic centre. The structure of mouse stearoyl-CoA desaturase (SDC) revealed that TM2 and TM4 are longer than TM1 and TM3 and protrude into the cytosolic domain, providing three of the nine histidine residues that coordinate the two metal ions, while the other histidine residues are provided by the soluble domain in this enzyme.


Pssm-ID: 425713 [Multi-domain]  Cd Length: 252  Bit Score: 46.96  E-value: 8.76e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490   11 AWQLVAVTLLMTHVtIIGVTVYLHRYSAHRSL----ELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKceTVDDPHS 86
Cdd:pfam00487   1 SWLALLLALLLGLF-LLGITGSLAHEASHGALfkkrRLNRWLNDLLGRLAGLPLGISYSAWRIAHLVHHRY--TNGPDKD 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490   87 PVIKGLSTVLRKGAELYRAEAENPETLRIYGKNCPDDWIERNLYTRYP----------LLGVALMGVIDLLLFGTIGITI 156
Cdd:pfam00487  78 PDTAPLASRFRGLLRYLLRWLLGLLVLAWLLALVLPLWLRRLARRKRPiksrrrrwrlIAWLLLLAAWLGLWLGFLGLGG 157

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939351490  157 WAIQMMWIPFWAAGVINGL--------GHAVGYRNFECRDAATNLVPW-GIIVGGEELHNNHHTYPN 214
Cdd:pfam00487 158 LLLLLWLLPLLVFGFLLALifnylehyGGDWGERPVETTRSIRSPNWWlNLLTGNLNYHIEHHLFPG 224
DDE_Tnp_ISL3 pfam01610
Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains ...
 271-387 5.42e-04

Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains transposases for IS204, IS1001, IS1096 and IS1165.


Pssm-ID: 426344  Cd Length: 238  Bit Score: 41.04  E-value: 5.42e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  271 RFQIMAqyrklvigpLVKQELEKVDHSVRHQFH--------RAKRLLSRETSLLEDRHHLRIQSMLEHSHALTVIYEKRL 342
Cdd:pfam01610  81 RFHVVK---------LANEALDEVRRREQKELKerlrkglkGSRWLLLKNPENLTEEQKQRLDELLDLSPKLARAYLLKE 151

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 939351490  343 ALQQIWLKTSSngHDMLAAIKEWVHEAEASGIQSLRDFAHQLKTY 387
Cdd:pfam01610 152 ALRQIWRYADR--AEARKFLDEWINWARRSGLPPFKKLAKTLRNH 194
 
Name Accession Description Interval E-value
Delta9-FADS-like cd03505
The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl ...
 12-242 1.55e-47

The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like CD includes the delta-9 and delta-11 acyl CoA desaturases found in various eukaryotes including vertebrates, insects, higher plants, and fungi. The delta-9 acyl-lipid desaturases are found in a wide range of bacteria. These enzymes play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. Acyl-CoA desaturases are the enzymes involved in the CoA-bound desaturation of fatty acids. Mammalian stearoyl-CoA delta-9 desaturase is a key enzyme in the biosynthesis of monounsaturated fatty acids, and in yeast, the delta-9 acyl-CoA desaturase (OLE1) reaction accounts for all de nova unsaturated fatty acid production in Saccharomyces cerevisiae. These non-heme, iron-containing, ER membrane-bound enzymes are part of a three-component enzyme system involving cytochrome b5, cytochrome b5 reductase, and the delta-9 fatty acid desaturase. This complex catalyzes the NADH- and oxygen-dependent insertion of a cis double bond between carbons 9 and 10 of the saturated fatty acyl substrates, palmitoyl (16:0)-CoA or stearoyl (18:0)-CoA, yielding the monoenoic products palmitoleic (16:l) or oleic (18:l) acids, respectively. In cyanobacteria, the biosynthesis of unsaturated fatty acids is initiated by delta 9 acyl-lipid desaturase (DesC) which introduces the first double bond at the delta-9 position of a saturated fatty acid that has been esterified to a glycerolipid. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXXH, HXXHH, and H/QXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase. Some eukaryotic (Fungi, Euglenozoa, Mycetozoa, Rhodophyta) desaturase domains have an adjacent C-terminal cytochrome b5-like domain.


Pssm-ID: 239582  Cd Length: 178  Bit Score: 160.03  E-value: 1.55e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPvikg 91
Cdd:cd03505    2 WATLVFLVLYYLLTGLGITAGYHRLWAHRSFKAPKPLRIFLAILGSLAGQGSPLWWVADHRLHHRYSDTDGDPHSP---- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  92 lstvlRKGaelyraeaenpetlriygkncpddwiernlytrypllgvalmgvidlLLFGTIGITIWAIQMMWIPFWAAGV 171
Cdd:cd03505   78 -----KRG-----------------------------------------------FWFSHVGWLGGLLRIVLVLHATWLV 105

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939351490 172 iNGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIKVFSFLRLAK 242
Cdd:cd03505  106 -NSLAHMWGYRPYDTRDTSRNNWWVALLTFGEGWHNNHHAFPGDARNGLKWYQIDPTKWVIRLLEKLGLAW 175
OLE1 COG1398
Fatty-acid desaturase [Lipid transport and metabolism];
12-252 2.32e-36

Fatty-acid desaturase [Lipid transport and metabolism];


Pssm-ID: 441008  Cd Length: 286  Bit Score: 133.80  E-value: 2.32e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFrFWLWLTTAQNTR-EWTAIHRKHHAKCETVDDPHSPVIK 90
Cdd:COG1398   43 WSAVALALVLYVLTGLGITVGYHRLFSHRSFKTPRWLEYLL-AILGALALQGGPlWWVADHRRHHRHSDTEGDPHSPKLR 121

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  91 GL--STVlrkGAELYRAEAENPEtlriygKNCPDD-------WIERNLYTRYPLLGVALMGVIDLLLFGTIGITIWAI-- 159
Cdd:COG1398  122 GFwwSHM---GWMLREDPTPNDY------RYAPDLakdpelrWLDRYYLLLQLALGLLLPALLGGLLGGGWSGLLWGGfv 192

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490 160 ------QMMWipfwaagVINGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIK 233
Cdd:COG1398  193 rtvllhHGTW-------FINSLAHVWGYRPFETRDTSRNNWWLALLTFGEGWHNNHHAFPTSARHGLRWWEIDPTWWLIR 265

                        250       260
                 ....*....|....*....|
gi 939351490 234 VFSFLRLA-KVQRVAPIAHR 252
Cdd:COG1398  266 LLEKLGLAwDVRRPPAERIA 285
PLN02220 PLN02220
delta-9 acyl-lipid desaturase
 12-241 1.84e-16

delta-9 acyl-lipid desaturase


Pssm-ID: 177866 [Multi-domain]  Cd Length: 299  Bit Score: 79.08  E-value: 1.84e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  12 WQLVAVTLLMTHVTIIGVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPvIKG 91
Cdd:PLN02220  55 WEALRFGLILYIVTGLSITFSYHRNLAHRSFKLPKWLEYPFAYSALFALQGDPIDWVSTHRFHHQFTDSDRDPHSP-IEG 133

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  92 L--STVLrkgaelyraeaenpetlriygkncpddWIERNLYTRYPLLGVAlmGVIDL-------LLFGTIGITI---WAI 159
Cdd:PLN02220 134 FwfSHVL---------------------------WIFDTSYIREKCGGRD--NVMDLkqqwfyrFLRKTIGLHIlmfWTL 184

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490 160 QMMW--IPF--WAAGV-----------INGLGHAVGYRNFECRDAATNLVPWGIIVGGEELHNNHHTYPNSAKLSVKKWE 224
Cdd:PLN02220 185 LYLWggLPYltWGVGVggaigyhvtwlINSACHIWGSRTWKTKDTSRNVWWLSLFTMGESWHNNHHAFESSARQGLEWWQ 264

                        250
                 ....*....|....*..
gi 939351490 225 FDLGWAWIKVFSFLRLA 241
Cdd:PLN02220 265 IDITWYLIRFFEVLGLA 281
Membrane-FADS-like cd01060
The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane ...
 15-89 3.46e-06

The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane hydroxylases, beta carotene ketolases (CrtW-like), hydroxylases (CrtR-like), and other related proteins. They are present in all groups of organisms with the exception of archaea. Membrane FADSs are non-heme, iron-containing, oxygen-dependent enzymes involved in regioselective introduction of double bonds in fatty acyl aliphatic chains. They play an important role in the maintenance of the proper structure and functioning of biological membranes. Alkane hydroxylases are bacterial, integral-membrane di-iron enzymes that share a requirement for iron and oxygen for activity similar to that of membrane FADSs, and are involved in the initial oxidation of inactivated alkanes. Beta-carotene ketolase and beta-carotene hydroxylase are carotenoid biosynthetic enzymes for astaxanthin and zeaxanthin, respectively. This superfamily domain has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of these sequences also reveals three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXX(X)H, HXX(X)HH, and HXXHH (an additional conserved histidine residue is seen between clusters 2 and 3). Spectroscopic and genetic evidence point to a nitrogen-rich coordination environment located in the cytoplasm with as many as eight histidines coordinating the two iron ions and a carboxylate residue bridging the two metals in the Pseudomonas oleovorans alkane hydroxylase (AlkB). In addition, the eight histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase.


Pssm-ID: 238511 [Multi-domain]  Cd Length: 122  Bit Score: 45.92  E-value: 3.46e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 939351490  15 VAVTLLMTHVTIIGVTVYLHRYsAHRSLELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETV-DDPHSPVI 89
Cdd:cd01060    1 LLLALLLGLLGGLGLTVLAHEL-GHRSFFRSRWLNRLLGALLGLALGGSYGWWRRSHRRHHRYTNTPgKDPDSAVN 75
FA_desaturase pfam00487
Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a ...
 11-214 8.76e-06

Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a double bond at the delta position of fatty acids. There seem to be two distinct families of fatty acid desaturases which do not seem to be evolutionary related: Family 1 composed of Stearoyl-CoA desaturases (SCD) and Family 2 composed of Bacterial fatty acid desaturases, Plant stearoyl-acyl-carrier-protein desaturase and Cyanobacterial DesA. Members of this entry are ER integral membrane proteins that share the same mushroom-shaped fold consisting of four transmembrane helices (TM1-TM4) which anchor them to the membrane, capped by a cytosolic domain containing a unique 9-10 histidine- coordinating di metal (di-iron) catalytic centre. The structure of mouse stearoyl-CoA desaturase (SDC) revealed that TM2 and TM4 are longer than TM1 and TM3 and protrude into the cytosolic domain, providing three of the nine histidine residues that coordinate the two metal ions, while the other histidine residues are provided by the soluble domain in this enzyme.


Pssm-ID: 425713 [Multi-domain]  Cd Length: 252  Bit Score: 46.96  E-value: 8.76e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490   11 AWQLVAVTLLMTHVtIIGVTVYLHRYSAHRSL----ELNAGLKHFFRFWLWLTTAQNTREWTAIHRKHHAKceTVDDPHS 86
Cdd:pfam00487   1 SWLALLLALLLGLF-LLGITGSLAHEASHGALfkkrRLNRWLNDLLGRLAGLPLGISYSAWRIAHLVHHRY--TNGPDKD 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490   87 PVIKGLSTVLRKGAELYRAEAENPETLRIYGKNCPDDWIERNLYTRYP----------LLGVALMGVIDLLLFGTIGITI 156
Cdd:pfam00487  78 PDTAPLASRFRGLLRYLLRWLLGLLVLAWLLALVLPLWLRRLARRKRPiksrrrrwrlIAWLLLLAAWLGLWLGFLGLGG 157

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939351490  157 WAIQMMWIPFWAAGVINGL--------GHAVGYRNFECRDAATNLVPW-GIIVGGEELHNNHHTYPN 214
Cdd:pfam00487 158 LLLLLWLLPLLVFGFLLALifnylehyGGDWGERPVETTRSIRSPNWWlNLLTGNLNYHIEHHLFPG 224
DDE_Tnp_ISL3 pfam01610
Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains ...
 271-387 5.42e-04

Transposase; Transposase proteins are necessary for efficient DNA transposition. Contains transposases for IS204, IS1001, IS1096 and IS1165.


Pssm-ID: 426344  Cd Length: 238  Bit Score: 41.04  E-value: 5.42e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939351490  271 RFQIMAqyrklvigpLVKQELEKVDHSVRHQFH--------RAKRLLSRETSLLEDRHHLRIQSMLEHSHALTVIYEKRL 342
Cdd:pfam01610  81 RFHVVK---------LANEALDEVRRREQKELKerlrkglkGSRWLLLKNPENLTEEQKQRLDELLDLSPKLARAYLLKE 151

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 939351490  343 ALQQIWLKTSSngHDMLAAIKEWVHEAEASGIQSLRDFAHQLKTY 387
Cdd:pfam01610 152 ALRQIWRYADR--AEARKFLDEWINWARRSGLPPFKKLAKTLRNH 194
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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