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Conserved domains on  [gi|872679342|ref|WP_048543142|]
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MULTISPECIES: methylated-DNA--[protein]-cysteine S-methyltransferase [Bacillus cereus group]

Protein Classification

methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11417447)

methylated-DNA--[protein]-cysteine S-methyltransferase is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA; it repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme

CATH:  3.30.160.70|1.10.10.10
EC:  2.1.1.63
Gene Ontology:  GO:0003908|GO:0003677|GO:0046872|GO:0006281|GO:0032259
PubMed:  17500045|17485252
SCOP:  4000565|4000678

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-150 1.77e-76

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 224.37  E-value: 1.77e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE----------HKNEMIDQCINELDEYFKGKRREFTVPLSAEGTAFQ 70
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEAllarfpaalrEDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  71 KNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK 150
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-150 1.77e-76

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 224.37  E-value: 1.77e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE----------HKNEMIDQCINELDEYFKGKRREFTVPLSAEGTAFQ 70
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEAllarfpaalrEDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  71 KNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK 150
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 1-147 2.39e-61

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 186.03  E-value: 2.39e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE----HKNEMIDQCINELDEYFKGKRREFTVPLSAEGTAFQKNVWDA 76
Cdd:PRK00901   2 NNIYFYETPIGKIGIAENGTAITHLCFGEDKIPKdvtiLETDLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKA 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 872679342  77 LYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:PRK00901  82 LQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 68-148 6.57e-45

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 141.73  E-value: 6.57e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   68 AFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 872679342  148 I 148
Cdd:pfam01035  81 V 81
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 67-146 9.09e-42

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 133.59  E-value: 9.09e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   67 TAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHE 146
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 69-147 7.44e-39

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 126.44  E-value: 7.44e-39
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 872679342  69 FQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-150 1.77e-76

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 224.37  E-value: 1.77e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE----------HKNEMIDQCINELDEYFKGKRREFTVPLSAEGTAFQ 70
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEAllarfpaalrEDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  71 KNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK 150
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 1-147 2.39e-61

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 186.03  E-value: 2.39e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE----HKNEMIDQCINELDEYFKGKRREFTVPLSAEGTAFQKNVWDA 76
Cdd:PRK00901   2 NNIYFYETPIGKIGIAENGTAITHLCFGEDKIPKdvtiLETDLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKA 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 872679342  77 LYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:PRK00901  82 LQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 68-148 6.57e-45

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 141.73  E-value: 6.57e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   68 AFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 872679342  148 I 148
Cdd:pfam01035  81 V 81
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 67-146 9.09e-42

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 133.59  E-value: 9.09e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   67 TAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHE 146
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
45-149 5.60e-39

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 129.60  E-value: 5.60e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  45 NELDEYFKGKRREF-TVPLSAEGTAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVI 123
Cdd:PRK10286  64 DKLRDYFAGNLSIIdTLPTATGGTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVI 143

                         90       100
                 ....*....|....*....|....*.
gi 872679342 124 GKSGKLVGYAGGLWRKEWLLKHEGIL 149
Cdd:PRK10286 144 GRNGTMTGYAGGVQRKEWLLRHEGYL 169
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 69-147 7.44e-39

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 126.44  E-value: 7.44e-39
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 872679342  69 FQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEG 147
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
9-146 8.38e-30

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 110.65  E-value: 8.38e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342   9 ELGLLEITANDKGITSV---------------IFVDERQEEHKNEM---IDQCINELDeyfkGKRREFTVPLSAEGTAFQ 70
Cdd:PRK15435 197 ELGRCLVAESERGICAIllgdddaaliselqqMFPAADNAPADLTFqqhVREVIASLN----QRDTPLTLPLDIRGTAFQ 272

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 872679342  71 KNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHE 146
Cdd:PRK15435 273 QQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGALSGYRWGVSRKAQLLRRE 348
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 40-146 1.40e-26

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 102.05  E-value: 1.40e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  40 IDQCINELDEYFKGKRREFTVPLSAEGTAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPC 119
Cdd:COG2169  243 FEQLVAEVVGFVEGPLLGLDLPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAIPC 322

                         90       100
                 ....*....|....*....|....*..
gi 872679342 120 HRVIGKSGKLVGYAGGLWRKEWLLKHE 146
Cdd:COG2169  323 HRVVRADGALSGYRWGVERKRALLERE 349
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 64-148 6.65e-13

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 60.97  E-value: 6.65e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  64 AEGTAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKL-VGYAGGLWRKEWL 142
Cdd:COG3695    1 MEMEEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLsPGHAGGAEEQREL 80


                 ....*.
gi 872679342 143 LKHEGI 148
Cdd:COG3695   81 LEAEGV 86
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 62-148 1.88e-10

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 55.90  E-value: 1.88e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 872679342  62 LSAEG-TAFQKNVWDALY-TIPYGVSASYLDIAeKVGNTKAvRAIGGANSRNPISIIVPCHRVIGKSGKLVgYAGGLWRK 139
Cdd:PRK03887  85 LSFEGlTPFERKVYEWLTkNVKRGEVITYGELA-KALNTSP-RAVGGAMKRNPYPIIVPCHRVVGRKNPGL-YTPKPEYK 161


                 ....*....
gi 872679342 140 EWLLKHEGI 148
Cdd:PRK03887 162 KFLLEVEGV 170
Methyltransf_1N pfam02870
6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the ...
 1-63 2.52e-04

6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the N-terminal ribonuclease-like domain associated with 6-O-methylguanine DNA methyltransferase activity. The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (also known as O-6-methylguanine-DNA-alkyltransferase)


Pssm-ID: 397139 [Multi-domain]  Cd Length: 77  Bit Score: 37.73  E-value: 2.52e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 872679342    1 MYQAYYESELGLLEITANDKGITSVIFVDERQEE-------HKNEMIDQCINE----LDEYFKGKR-REFTVPLS 63
Cdd:pfam02870   2 LYYTLIDSPLGRLLLAGDERGLTAIDFLDKDYALrkelpkvLPQPELLPALALlvqaLEEYFAGELkPEFTLPLD 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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