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Conserved domains on  [gi|829939530|ref|WP_047367650|]
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MULTISPECIES: oxygen-insensitive NAD(P)H nitroreductase [Enterobacter]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10793524)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 1.21e-156

oxygen-insensitive NAD(P)H nitroreductase;


:

Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 432.09  E-value: 1.21e-156
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   1 MDIISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  81 VVVFCAKTAMDDAWLERVVDQEEADGRFNTPEAKAANHKGRTYFADMHRVDLKDDDQWMAKQVYLNVGNFLLGVGAMGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 829939530 161 AVPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEDFNATLPKSRLPLSTIVTEC 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
 
Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 1.21e-156

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 432.09  E-value: 1.21e-156
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   1 MDIISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  81 VVVFCAKTAMDDAWLERVVDQEEADGRFNTPEAKAANHKGRTYFADMHRVDLKDDDQWMAKQVYLNVGNFLLGVGAMGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 829939530 161 AVPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEDFNATLPKSRLPLSTIVTEC 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 2-213 2.81e-63

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 193.62  E-value: 2.81e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   2 DIISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyVFNERKMLDASHV 81
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAA----WFNQPQIKDASHV 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  82 VVFCAKTAmddawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvdlkdddqWMAKQVYLNVGNFLLGVGAMGLDA 161
Cdd:cd02149   77 VVFLAKKD------------------------------------------------WSAKQTYIALGNMLLAAAMLGIDS 108

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 829939530 162 VPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEdfnaTLPKSRLPLSTI 213
Cdd:cd02149  109 CPIEGFDPAKLDEILGLDEKGYKISVMVAFGYRSEE----KLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 3-214 2.65e-36

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 124.97  E-value: 2.65e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   3 IISVALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTyvfNERKMLDASHVV 82
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAEA---NQEWVADAPVLI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  83 VFCAKTAMDDawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvdlKDDDQWMAKQVYLNVGNFLLGVGAMGLDAV 162
Cdd:COG0778   77 VVCADPDRSE----------------------------------------KVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 829939530 163 PIEGFDAAILDEEFGLKEkGFTSLVVVPVGHHSVEDFnatlPKSRLPLSTIV 214
Cdd:COG0778  117 WIGGFDPEKVRELLGLPE-GEEPVALLALGYPAEELN----PRPRKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 7-193 7.13e-27

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 100.93  E-value: 7.13e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530    7 ALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASHVV---- 82
Cdd:pfam00881   1 IRQRRSVRKFDP-EPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRdanl 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   83 VFCAKTAMDDAWLERVVDQEEADGRFNTPEakaanhkgrtyfadmhrvdlkDDDQWMAKQVYLNVGNFLLGVGAMGLDAV 162
Cdd:pfam00881  80 KLLLQDFLRGAPVLIVITASLSTYLRKAAE---------------------RAYREALLDAGAAAQNLLLAATSLGLGSC 138

                         170       180       190
                  ....*....|....*....|....*....|.
gi 829939530  163 PIEGFDAAILDEEFGLKEKGFTsLVVVPVGH 193
Cdd:pfam00881 139 PIGGFDAAAVRELLGLPDDERL-VGLIAVGY 168
 
Name Accession Description Interval E-value
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
1-217 1.21e-156

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 432.09  E-value: 1.21e-156
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   1 MDIISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASH 80
Cdd:PRK11053   1 MDIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYAFNERKILDASH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  81 VVVFCAKTAMDDAWLERVVDQEEADGRFNTPEAKAANHKGRTYFADMHRVDLKDDDQWMAKQVYLNVGNFLLGVGAMGLD 160
Cdd:PRK11053  81 VVVFCAKTDMDDAYLELVLEQEDADGRFATEEAKAAQDKGRRFFADMHRKELKDLQHWMEKQVYLALGNLLLGAAALGID 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 829939530 161 AVPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEDFNATLPKSRLPLSTIVTEC 217
Cdd:PRK11053 161 ATPIEGFDAAILDAEFGLREKGLTSSVVVPLGYHSEEDFNAKLPKSRLPQETIFTEI 217
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 2-213 2.81e-63

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 193.62  E-value: 2.81e-63
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   2 DIISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyVFNERKMLDASHV 81
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAA----WFNQPQIKDASHV 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  82 VVFCAKTAmddawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvdlkdddqWMAKQVYLNVGNFLLGVGAMGLDA 161
Cdd:cd02149   77 VVFLAKKD------------------------------------------------WSAKQTYIALGNMLLAAAMLGIDS 108

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 829939530 162 VPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEdfnaTLPKSRLPLSTI 213
Cdd:cd02149  109 CPIEGFDPAKLDEILGLDEKGYKISVMVAFGYRSEE----KLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 3-214 2.65e-36

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 124.97  E-value: 2.65e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   3 IISVALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTyvfNERKMLDASHVV 82
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAEA---NQEWVADAPVLI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  83 VFCAKTAMDDawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvdlKDDDQWMAKQVYLNVGNFLLGVGAMGLDAV 162
Cdd:COG0778   77 VVCADPDRSE----------------------------------------KVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 829939530 163 PIEGFDAAILDEEFGLKEkGFTSLVVVPVGHHSVEDFnatlPKSRLPLSTIV 214
Cdd:COG0778  117 WIGGFDPEKVRELLGLPE-GEEPVALLALGYPAEELN----PRPRKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 7-193 7.13e-27

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 100.93  E-value: 7.13e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530    7 ALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASHVV---- 82
Cdd:pfam00881   1 IRQRRSVRKFDP-EPVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRdanl 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   83 VFCAKTAMDDAWLERVVDQEEADGRFNTPEakaanhkgrtyfadmhrvdlkDDDQWMAKQVYLNVGNFLLGVGAMGLDAV 162
Cdd:pfam00881  80 KLLLQDFLRGAPVLIVITASLSTYLRKAAE---------------------RAYREALLDAGAAAQNLLLAATSLGLGSC 138

                         170       180       190
                  ....*....|....*....|....*....|.
gi 829939530  163 PIEGFDAAILDEEFGLKEKGFTsLVVVPVGH 193
Cdd:pfam00881 139 PIGGFDAAAVRELLGLPDDERL-VGLIAVGY 168
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 8-193 3.54e-23

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 90.43  E-value: 3.54e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   8 LKRHSTKAFDPTkkltAEEAEKIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAAGtyvfNERKMLDASHVVVF 84
Cdd:cd02062    2 KTRRSIRKFTDK----PVPEEKLRKILeaaRLAPSAGNLQPWRFIVVRDREKKEKLAKLAAP----NQKFIAGAPVVIVV 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  85 CAktamddawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvDLKDDDQWMAKQVYLNVGNFLLGVGAMGLDAVPI 164
Cdd:cd02062   74 VA--------------------------------------------DPDKSRPWALEDAGAAAQNLLLAAAALGLGSCWI 109

                        170       180       190
                 ....*....|....*....|....*....|.
gi 829939530 165 EGFD--AAILDEEFGLKEkGFTSLVVVPVGH 193
Cdd:cd02062  110 GGFDfrEDKVRELLGIPE-NLRPVALIAIGY 139
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 9-213 1.68e-22

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 88.84  E-value: 1.68e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   9 KRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyvFNERKMLDASHVVVFCAkt 88
Cdd:cd02137    6 SRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAA-----YNQPQVTTASAVILVLG-- 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  89 amddawlervvdqeeadgrfntpeakaanhkgrtyfadmhrvdlkDDDQWMAKQvylnvgNFLLGVGAMGLDAVPIEGFD 168
Cdd:cd02137   79 ---------------------------------------------DLNAGLAAM------NLMLAAKAKGYDTCPMGGFD 107

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 829939530 169 AAILDEEFGLkEKGFTSLVVVPVGHHSVEDfnatLPKSRLPLSTI 213
Cdd:cd02137  108 KEKVAELLNL-PDRYVPVLLIAIGKAADKA----PRSGRLPVDEV 147
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 8-214 1.31e-15

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 71.42  E-value: 1.31e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   8 LKRHSTKAFDPtKKLTAEEaekIKTLL---QYSPSSTNSQPWHFIVASTE-EGKARVAKSAAGtyvFNERKMLDASHVVV 83
Cdd:cd02138    3 AERWSPRAFSP-EPISEED---LLSLFeaaRWAPSCFNEQPWRFVVARRDtEAFEKLLDLLAE---GNQSWAKNAPVLIV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  84 FCAKTAMDDawlervvdqeeadgrfntpeakaaNHKGRTYfadmHRVDLKdddqwMAkqvylnVGNFLLGVGAMGLDAVP 163
Cdd:cd02138   76 VLAKTEFDH------------------------NGKPNRY----ALFDTG-----AA------VANLALQATALGLVVHQ 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 829939530 164 IEGFDAAILDEEFGLKEkGFTSLVVVPVGHHS-VEDFNATL------PKSRLPLSTIV 214
Cdd:cd02138  117 MAGFDPEKAKEALGIPD-EYEPITMIAIGYPGdPESLPEKLlereeaPRTRKPLSEIV 173
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 7-210 2.73e-13

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 65.80  E-value: 2.73e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   7 ALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyvFNERKMLDASHVVVFCA 86
Cdd:cd03370    5 IESRRSIRKYTQ-EPVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAA-----YGQAQVTSAPAVIVIYS 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  87 KtaMDDAwLERVVDQEEADGrfnTPEAKAANHKG-RTYFADMhrvDLKDDDQWMAKQVYLNVGNFLLGVGAMGLDAVPIE 165
Cdd:cd03370   79 D--MEDA-LANLEETIHPGL---SEERRQREAAGlRGAFGKM---SVEQRGQWGLAQANIALGFLLLAAQSLGYDTSPML 149

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 829939530 166 GFDAAILDEEFGLKEK-GFTSLvvVPVGHHSVEDFnatlPKSRLPL 210
Cdd:cd03370  150 GFDPEKVKALLGLPEHvTIAAL--VALGKPAEEGY----PHHRHSL 189
RutE-like cd02148
nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family ...
 11-193 5.91e-12

nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. RutE is involved in the utilization of uracil as the sole nitrogen source; it appears to have the same function as YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid.


Pssm-ID: 380323 [Multi-domain]  Cd Length: 186  Bit Score: 61.89  E-value: 5.91e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  11 HSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGtyvFNERKMLDASHVVVFCAKTAM 90
Cdd:cd02148   10 RTHNAWED-KPVSDEELRAIYDLAKWGPTAANCSPARIVFVRSAEAKERLVPHLSE---GNREKTMAAPVTAILAYDTEF 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  91 DDaWLERVVdqeeadgrfntPEAKAanhkgRTYFADmhrvdlkdDDQWMAKQVYLNVGN-----FLLGVGAMGLDAVPIE 165
Cdd:cd02148   86 YE-HLPRLF-----------PHGDA-----RSWFFG--------SGPARAEETAFRNASlqaayFILAARALGLDCGPMS 140

                        170       180
                 ....*....|....*....|....*...
gi 829939530 166 GFDAAILDEEFgLKEKGFTSLVVVPVGH 193
Cdd:cd02148  141 GFDAAGVDAEF-FAGTRWKSNLVVNLGY 167
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 20-193 2.51e-11

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 60.21  E-value: 2.51e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  20 KKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTyvfNERKMLDASHVVVFcaktAMDDAW---LE 96
Cdd:PRK05365  25 EPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKERLRPALSEG---NLAKTLAAPVTAIV----AYDTEFhehLP 97

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  97 RVVDQEEADGRFNTPEAKAAnhkgRTYFADMHrvdlkdddqwmakqvyLNVGNFLLGVGAMGLDAVPIEGFDAAILDEEF 176
Cdd:PRK05365  98 KLFPHADARSWFAGNPALAE----ETAFRNSS----------------LQGAYLILAARALGLDAGPMSGFDAAAVDAEF 157

                        170
                 ....*....|....*..
gi 829939530 177 gLKEKGFTSLVVVPVGH 193
Cdd:PRK05365 158 -FAGTTWKSNFLVNIGY 173
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 2-94 3.70e-11

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 58.94  E-value: 3.70e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   2 DIISVALKRHSTKAFDPTKKltaeEAEKIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYvfnerkmlDA 78
Cdd:cd20609    1 DFLELAKKRYSVRKFSDKPV----EKEKLDKILeagRLAPTAVNYQPQRILVVRSEEALEKLAKATPRFF--------GA 68

                         90
                 ....*....|....*.
gi 829939530  79 SHVVVFCAKTamDDAW 94
Cdd:cd20609   69 PLVIVVCYDK--DESW 82
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 6-96 4.04e-10

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 56.06  E-value: 4.04e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   6 VALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASteeGKARvAKSAAGTYvfnerkmlDASHVVVFC 85
Cdd:cd02136    1 AIKSRRSVRAFKD-KPVPKETIEKILEAARRAPSGKNTQPWRVYVVT---GKAR-ERLKKAFF--------GAPVALFLT 67

                         90
                 ....*....|.
gi 829939530  86 AKTAMDDAWLE 96
Cdd:cd02136   68 MDKVLGPWSWF 78
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 3-86 2.62e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 54.01  E-value: 2.62e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   3 IISVALKRHSTKAFDPTkkltAEEAEKIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyvFNERKMLDAS 79
Cdd:cd02139    1 VYEAIKKRRSIRKYKPT----PVEEEKLLRILeaaRLAPSAKNRQPWRFIVVKDKELKEKLAEAA-----NGQKFIAEAP 71


                 ....*..
gi 829939530  80 HVVVFCA 86
Cdd:cd02139   72 VVIVACA 78
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 26-96 1.67e-08

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 51.57  E-value: 1.67e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 829939530  26 EAEKIKTLL---QYSPSSTNSQPWHFIVASTEEGKARVAKSAAgtYVFNERKmlDASHVVVFCAKTAmDDAWLE 96
Cdd:cd20608   19 EEEKLEKILeaaRLAPSWANKQCWRFIVVTDKETLSELAKKES--PSNGWLK--DAPVIIVVCADPK-DSGWLN 87
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 6-96 1.94e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 51.76  E-value: 1.94e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   6 VALKRHSTKAFDPTKKltaeEAEKIKTLLQY---SPSSTNSQPWHFIVASTEEGKARVA--KSAAGTYVfnerkmLDASH 80
Cdd:cd02151    2 LLKKRRSIRKYTDEPI----EEEKLEEILEAallAPSSRNSRPVEFIVVDDKETLKKLSecKPHGSAFL------KGAPA 71

                         90
                 ....*....|....*.
gi 829939530  81 VVVFCAKTAMDDAWLE 96
Cdd:cd02151   72 AIVVLADTEKSDTWIE 87
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 8-193 8.29e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 49.91  E-value: 8.29e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   8 LKRHSTKAFDPtKKLtaeEAEKIKTLLQ---YSPSSTNSQPWHFIVASTEEGKARVAKsaAGTYvfneRKML-DASHVVV 83
Cdd:cd02150    2 LTRRSIRKYTD-KPV---EEEDIEKLLRaamAAPSAGNQQPWHFIVVTDREKLDKIAE--AHPY----GKMLkEAPLAIV 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  84 FCA---KTAMDDAWlerVVDqeeadgrfntpeAKAAnhkgrtyfadmhrvdlkdddqwmakqvylnVGNFLLGVGAMGLD 160
Cdd:cd02150   72 VCGdpsKEKAPGYW---VQD------------CSAA------------------------------TENILLAAHALGLG 106

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 829939530 161 AV-----PIEGFDAAIlDEEFGLKEkGFTSLVVVPVGH 193
Cdd:cd02150  107 AVwlgvyPFEERVKAI-REILNIPE-NIIPFCVIALGY 142
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 4-216 3.81e-06

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 46.08  E-value: 3.81e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   4 ISVALKRHSTKAFDPtKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAagtyvFNERKMLDASHVVV 83
Cdd:cd02146    2 IETILNHRSVRKFTD-EPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELA-----GNQPYVAQAPVFLV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530  84 FCAKTAMDDAWLERvvdQEEADGRFNTPEAkaanhkgrTYFADMhrvdlkddDQWMAKQvylnvgNFLLGVGAMGLDAVP 163
Cdd:cd02146   76 FCADLYRHQKIAEE---AGGKDVGLDYLES--------FLVGVV--------DAALAAQ------NALVAAESLGLGIVY 130

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 829939530 164 IEGF--DAAILDEEFGLKEKGFtSLVVVPVGHhsvEDFNATLpKSRLPLSTIVTE 216
Cdd:cd02146  131 IGGIrnNPEEVIELLGLPEYVF-PLFGLTVGH---PDPTPEV-KPRLPLEAVVHE 180
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 9-63 1.47e-04

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 40.72  E-value: 1.47e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 829939530   9 KRHSTKAF--DPTKKltaEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKS 63
Cdd:cd20610    3 KRRSIRKFkpDPVPK---EDIEKILEAANWAPSGMNRQNWEFVVVKGGEKIEKIGIS 56
McbC_SagB-like_oxidoreductase cd02142
oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain ...
 148-193 7.63e-04

oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.


Pssm-ID: 380318  Cd Length: 200  Bit Score: 38.94  E-value: 7.63e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 829939530 148 GNFLLGVGAMGLDAVPIEGFDAAILDEEFGLKEKGFTSLVVVPVGH 193
Cdd:cd02142  155 QNLYLAATALGLGLCAIGAFDDDALRELLGLDEVEEVVLYAFVVGG 200
nitroreductase_FeS-like cd02143
nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family ...
 26-94 8.23e-04

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.


Pssm-ID: 380319 [Multi-domain]  Cd Length: 187  Bit Score: 38.99  E-value: 8.23e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 829939530  26 EAEKIKTLLQ---YSPSSTNSQPWHFIVAsteEGKARVAKSAAGTYVFNERKMLDASHVVVFCAKTAMDDAW 94
Cdd:cd02143   17 PRETLEKLLDiarYAPTGHNSQPVHWLVV---DDPEKVRRLAELVIDWMRELIKEDPELAGKLFLDGIVAAW 85
YdjA-like cd02135
nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the ...
 4-92 1.05e-03

nitroreductase family protein similar to Escherichia coli YdjA; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase YdjA from Escherichia coli. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380312 [Multi-domain]  Cd Length: 162  Bit Score: 38.35  E-value: 1.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 829939530   4 ISVALKRHSTKAFDPTKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVAsTEEGKARVAKSAAGTYVFNERKM-------- 75
Cdd:cd02135    1 LELIKTRRSIRKFKLTGAPPEEQLEELLEAAMWAPNHGKLEPWRFIVV-TGEGRERLAELLAAAAAARAPGAdpekleka 79

                         90       100
                 ....*....|....*....|.
gi 829939530  76 ----LDASHVVVFCAKTAMDD 92
Cdd:cd02135   80 rekaLRAPVVIAVVAKPDEDP 100
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 28-75 3.89e-03

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 37.13  E-value: 3.89e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 829939530  28 EKIKTLLQ---YSPSSTNSQPWHFIVASTEEGKARVAKSA-AGTYVFNERKM 75
Cdd:cd02144   22 EVIENAIRtagTAPSGANTQPWTFVVVSDPEIKRKIREAAeEEEKEFYEKRM 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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