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Conserved domains on  [gi|750203907|ref|WP_040507981|]
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methylated-DNA--[protein]-cysteine S-methyltransferase [Granulicatella elegans]

Protein Classification

methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11417447)

methylated-DNA--[protein]-cysteine S-methyltransferase is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA; it repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme

CATH:  3.30.160.70|1.10.10.10
EC:  2.1.1.63
Gene Ontology:  GO:0003908|GO:0003677|GO:0046872|GO:0006281|GO:0032259
PubMed:  17500045|17485252
SCOP:  4000565|4000678

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 2-159 3.13e-69

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 206.65  E-value: 3.13e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   2 IQTPLGEIRLRSDGKSLTGLWFIGQVNDTKFN--SDIEIKDDlPIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVW 79
Cdd:COG0350    7 FDTPLGPLLIAATDRGLCALSFGDDREEALLArfPAALREDP-PLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQRRVW 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  80 KILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLERGK 159
Cdd:COG0350   86 EALRKIPYGETVTYGELARAIGRPK-----AARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGAL 160
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 2-159 3.13e-69

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 206.65  E-value: 3.13e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   2 IQTPLGEIRLRSDGKSLTGLWFIGQVNDTKFN--SDIEIKDDlPIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVW 79
Cdd:COG0350    7 FDTPLGPLLIAATDRGLCALSFGDDREEALLArfPAALREDP-PLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQRRVW 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  80 KILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLERGK 159
Cdd:COG0350   86 EALRKIPYGETVTYGELARAIGRPK-----AARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGAL 160
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 2-157 1.32e-55

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 171.77  E-value: 1.32e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   2 IQTPLGEIRLRSDGKSLTGLWFIgqvNDTKFnSDIEIKDDlPIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVWKI 81
Cdd:PRK00901   7 YETPIGKIGIAENGTAITHLCFG---EDKIP-KDVTILET-DLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKA 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 750203907  82 LQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLER 157
Cdd:PRK00901  82 LQEIPYGETRSYKEIAVNIGNPK-----ACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 74-157 2.63e-45

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 142.88  E-value: 2.63e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   74 FQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLL 153
Cdd:pfam01035   2 FQRRVWEALRQIPYGKTTTYGEIAKLLGRPK-----AARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALL 76


                  ....
gi 750203907  154 QLER 157
Cdd:pfam01035  77 ELEG 80
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 72-156 8.72e-41

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 131.67  E-value: 8.72e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   72 TMFQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEH 151
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPK-----AARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEF 75


                  ....*
gi 750203907  152 LLQLE 156
Cdd:TIGR00589  76 LLEHE 80
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 74-157 4.40e-40

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 129.91  E-value: 4.40e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  74 FQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLL 153
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPK-----AARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELL 75


                 ....
gi 750203907 154 QLER 157
Cdd:cd06445   76 ELEG 79
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 2-159 3.13e-69

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 206.65  E-value: 3.13e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   2 IQTPLGEIRLRSDGKSLTGLWFIGQVNDTKFN--SDIEIKDDlPIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVW 79
Cdd:COG0350    7 FDTPLGPLLIAATDRGLCALSFGDDREEALLArfPAALREDP-PLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQRRVW 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  80 KILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLERGK 159
Cdd:COG0350   86 EALRKIPYGETVTYGELARAIGRPK-----AARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGAL 160
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 2-157 1.32e-55

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 171.77  E-value: 1.32e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   2 IQTPLGEIRLRSDGKSLTGLWFIgqvNDTKFnSDIEIKDDlPIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVWKI 81
Cdd:PRK00901   7 YETPIGKIGIAENGTAITHLCFG---EDKIP-KDVTILET-DLLKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKA 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 750203907  82 LQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLER 157
Cdd:PRK00901  82 LQEIPYGETRSYKEIAVNIGNPK-----ACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 74-157 2.63e-45

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 142.88  E-value: 2.63e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   74 FQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLL 153
Cdd:pfam01035   2 FQRRVWEALRQIPYGKTTTYGEIAKLLGRPK-----AARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALL 76


                  ....
gi 750203907  154 QLER 157
Cdd:pfam01035  77 ELEG 80
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 72-156 8.72e-41

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 131.67  E-value: 8.72e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907   72 TMFQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEH 151
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPK-----AARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEF 75


                  ....*
gi 750203907  152 LLQLE 156
Cdd:TIGR00589  76 LLEHE 80
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 74-157 4.40e-40

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 129.91  E-value: 4.40e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  74 FQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCHRVLGKNGALTGYAGGVHRKEHLL 153
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPK-----AARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELL 75


                 ....
gi 750203907 154 QLER 157
Cdd:cd06445   76 ELEG 79
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
52-156 8.55e-33

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 114.20  E-value: 8.55e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  52 LESYFSGE-QIPIKIPLQPKGTMFQERVWKILQEIPYGETMTYGEIAQRIAKEKgvetySAQAVGQAVGKNPISILIPCH 130
Cdd:PRK10286  66 LRDYFAGNlSIIDTLPTATGGTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPG-----AARAVGAANGSNPISIVVPCH 140

                         90       100
                 ....*....|....*....|....*.
gi 750203907 131 RVLGKNGALTGYAGGVHRKEHLLQLE 156
Cdd:PRK10286 141 RVIGRNGTMTGYAGGVQRKEWLLRHE 166
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
47-157 9.22e-29

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 108.34  E-value: 9.22e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  47 QVETWLEsyfsGEQIPIKIPLQPKGTMFQERVWKILQEIPYGETMTYGEIAQRIAKEKGVetysaQAVGQAVGKNPISIL 126
Cdd:PRK15435 248 EVIASLN----QRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAV-----RAVASACAANKLAIV 318

                         90       100       110
                 ....*....|....*....|....*....|.
gi 750203907 127 IPCHRVLGKNGALTGYAGGVHRKEHLLQLER 157
Cdd:PRK15435 319 IPCHRVVRGDGALSGYRWGVSRKAQLLRREA 349
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 43-158 9.11e-24

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 94.74  E-value: 9.11e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  43 PIFGQVETWLESYFSGEQIPIKIPLQPKGTMFQERVWKILQEIPYGETMTYGEIAQRIAKEKGVEtysaqAVGQAVGKNP 122
Cdd:COG2169  241 AAFEQLVAEVVGFVEGPLLGLDLPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVR-----AVAAACAANQ 315

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 750203907 123 ISILIPCHRVLGKNGALTGYAGGVHRKEHLLQLERG 158
Cdd:COG2169  316 LAVAIPCHRVVRADGALSGYRWGVERKRALLEREAA 351
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 74-156 1.06e-16

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 70.98  E-value: 1.06e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  74 FQERVWKILQEIPYGETMTYGEIAQRIAKEKGvetysAQAVGQAVGKNPISILIPCHRVLGKNGALT-GYAGGVHRKEHL 152
Cdd:COG3695    6 FYERVYEVVAQIPPGRVATYGDIAALAGLPRG-----ARQVGRALRALPEGSDLPWHRVVNADGRLSpGHAGGAEEQREL 80


                 ....
gi 750203907 153 LQLE 156
Cdd:COG3695   81 LEAE 84
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 72-156 6.75e-11

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 57.44  E-value: 6.75e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 750203907  72 TMFQERVWKILQE-IPYGETMTYGEIAqriakeKGVETySAQAVGQAVGKNPISILIPCHRVLGKNGaLTGYAGGVHRKE 150
Cdd:PRK03887  91 TPFERKVYEWLTKnVKRGEVITYGELA------KALNT-SPRAVGGAMKRNPYPIIVPCHRVVGRKN-PGLYTPKPEYKK 162


                 ....*.
gi 750203907 151 HLLQLE 156
Cdd:PRK03887 163 FLLEVE 168
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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