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Conserved domains on  [gi|739963995|ref|WP_037814267|]
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MULTISPECIES: GNAT family N-acetyltransferase [unclassified Streptomyces]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10006981)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
151-285 8.40e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


:

Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 58.94  E-value: 8.40e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 151 IKSIGPEQAQDFADVLRSAFNTSRPTrEYWHAMSAGPfyADARCLGAYDDqGSVAAVVTVWSA---GPGKPGLVEPMGVH 227
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGREA-ELVDRLREDP--AAGLSLVAEDD-GEIVGHVALSPVdidGEGPALLLGPLAVD 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 739963995 228 EVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGvatYKAAGFEVRPEVRDRI 285
Cdd:COG3153   77 PEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPF---YERFGFRPAGELGLTL 131
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
151-285 8.40e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 58.94  E-value: 8.40e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 151 IKSIGPEQAQDFADVLRSAFNTSRPTrEYWHAMSAGPfyADARCLGAYDDqGSVAAVVTVWSA---GPGKPGLVEPMGVH 227
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGREA-ELVDRLREDP--AAGLSLVAEDD-GEIVGHVALSPVdidGEGPALLLGPLAVD 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 739963995 228 EVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGvatYKAAGFEVRPEVRDRI 285
Cdd:COG3153   77 PEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPF---YERFGFRPAGELGLTL 131
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 160-275 1.08e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.66  E-value: 1.08e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995  160 QDFADVLRSAFNTSRPTREYWhAMSAGPFYADARCLGAYDDqGSVAAVVTVWSAGPGKP-GLVEPMGVHEVHRGRGYGRA 238
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEPLD-LLEDWDEDASEGFFVAEED-GELVGFASLSIIDDEPPvGEIEGLAVAPEYRGKGIGTA 79

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 739963995  239 ITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGF 275
Cdd:pfam00583  80 LLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 194-258 1.66e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.18  E-value: 1.66e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 739963995 194 CLGAYDDqGSVAAVVTVWSAGPGKPGL-VEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVC 258
Cdd:cd04301    1 FLVAEDD-GEIVGFASLSPDGSGGDTAyIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK03624 PRK03624
putative acetyltransferase; Provisional
 225-281 8.17e-03

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 36.06  E-value: 8.17e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 739963995 225 GVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGFEVRPEV 281
Cdd:PRK03624  75 AVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEEQDRI 131
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
151-285 8.40e-11

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 58.94  E-value: 8.40e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 151 IKSIGPEQAQDFADVLRSAFNTSRPTrEYWHAMSAGPfyADARCLGAYDDqGSVAAVVTVWSA---GPGKPGLVEPMGVH 227
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGREA-ELVDRLREDP--AAGLSLVAEDD-GEIVGHVALSPVdidGEGPALLLGPLAVD 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 739963995 228 EVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGvatYKAAGFEVRPEVRDRI 285
Cdd:COG3153   77 PEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPF---YERFGFRPAGELGLTL 131
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 206-283 3.19e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 53.12  E-value: 3.19e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 739963995 206 AVVTVWSAGPGKPGLVEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGFEVRPEVRD 283
Cdd:COG0456    1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPN 78
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
149-283 1.18e-07

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 50.38  E-value: 1.18e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 149 VRIKSIGPEQAQDFADVLRSA-------FNTSRPTREYWHAMSAGPFYADARCLGAyDDQGSVAAVVTV--WSAGPGKPG 219
Cdd:COG1247    2 MTIRPATPEDAPAIAAIYNEAiaegtatFETEPPSEEEREAWFAAILAPGRPVLVA-EEDGEVVGFASLgpFRPRPAYRG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 739963995 220 LVEP-MGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGFEVRPEVRD 283
Cdd:COG1247   81 TAEEsIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 189-287 4.21e-07

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 48.13  E-value: 4.21e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 189 YADARCLGAYDdQGSVAAVVTVWSaGPGKPGLVEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVA 268
Cdd:COG0454   31 LAGAEFIAVDD-KGEPIGFAGLRR-LDDKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIR 108

                         90
                 ....*....|....*....
gi 739963995 269 TYKAAGFEVRPEVRDRIRT 287
Cdd:COG0454  109 FYERLGFKEIERYVAYVGG 127
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
221-282 5.49e-07

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 46.44  E-value: 5.49e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 739963995 221 VEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGFEVRPEVR 282
Cdd:COG3393   18 ISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVGEYA 79
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 160-275 1.08e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 43.66  E-value: 1.08e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995  160 QDFADVLRSAFNTSRPTREYWhAMSAGPFYADARCLGAYDDqGSVAAVVTVWSAGPGKP-GLVEPMGVHEVHRGRGYGRA 238
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEPLD-LLEDWDEDASEGFFVAEED-GELVGFASLSIIDDEPPvGEIEGLAVAPEYRGKGIGTA 79

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 739963995  239 ITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGF 275
Cdd:pfam00583  80 LLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 194-258 1.66e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.18  E-value: 1.66e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 739963995 194 CLGAYDDqGSVAAVVTVWSAGPGKPGL-VEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVC 258
Cdd:cd04301    1 FLVAEDD-GEIVGFASLSPDGSGGDTAyIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 188-280 3.35e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 39.98  E-value: 3.35e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 188 FYADARCLGAYDDQGSVAAVVTvWSAGPGKPGLVEPMGVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGgv 267
Cdd:COG1246   23 LEEEIGEFWVAEEDGEIVGCAA-LHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSAAIH-- 99

                         90
                 ....*....|...
gi 739963995 268 aTYKAAGFEVRPE 280
Cdd:COG1246  100 -FYEKLGFEEIDK 111
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 191-277 7.95e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 37.82  E-value: 7.95e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995  191 DARCLGAYDDqGSVAAVVTVWSAGPGKPGLVEPMGVHEVHRGRGYGRAITVAGTAALREMGsssVRVCTPSSNVGGVATY 270
Cdd:pfam13508   2 GGRFFVAEDD-GKIVGFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGG---IKLLELETTNRAAAFY 77


                  ....*..
gi 739963995  271 KAAGFEV 277
Cdd:pfam13508  78 EKLGFEE 84
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 151-251 5.20e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 36.40  E-value: 5.20e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995  151 IKSIGPEQAQDFADVLRSAFNTSRP--TREYWHAMsagpfYADARCLGAYDD---QGSVAAV---VTVwsagPGKP---G 219
Cdd:pfam13527   1 IRPLTEDEFDEVLRLLEYAFQDEDSpeLREYFRPL-----LEEGRVLGAFDDgelVSTLALYpfeLNV----PGKTlpaA 71

                          90       100       110
                  ....*....|....*....|....*....|..
gi 739963995  220 LVEPMGVHEVHRGRGYGRAITVAGTAALREMG 251
Cdd:pfam13527  72 GITGVATYPEYRGRGVMSRLLRRSLEEMRERG 103
PRK03624 PRK03624
putative acetyltransferase; Provisional
 225-281 8.17e-03

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 36.06  E-value: 8.17e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 739963995 225 GVHEVHRGRGYGRAITVAGTAALREMGSSSVRVCTPSSNVGGVATYKAAGFEVRPEV 281
Cdd:PRK03624  75 AVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEEQDRI 131
Eis COG4552
Predicted acetyltransferase [General function prediction only];
150-250 8.73e-03

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 37.19  E-value: 8.73e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739963995 150 RIKSIGPEQAQDFADVLRSAFNtSRPTREYWHAMSagPFYADARCLGAYDDqGSVAAVVTVWSAGPGKPGLVEPMG---- 225
Cdd:COG4552    2 EIRPLTEDDLDAFARLLAYAFG-PEPDDEELEAYR--PLLEPGRVLGVFDD-GELVGTLALYPFTLNVGGARVPMAgitg 77

                         90       100
                 ....*....|....*....|....*..
gi 739963995 226 --VHEVHRGRGYGRAItvaGTAALREM 250
Cdd:COG4552   78 vaVAPEHRRRGVARAL---LREALAEL 101
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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