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Conserved domains on  [gi|727178801|ref|WP_033642100|]
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MULTISPECIES: type II secretion system pilot lipoprotein GspS [Serratia]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
T2SS_PulS_OutS super family cl22759
Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins ...
 11-120 1.14e-19

Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins from four gamma proteobacterial species: PulS protein of Klebsiella pneumoniae (P20440), the OutS protein of Erwinia chrysanthemi (Q01567) and Pectobacterium chrysanthemi, and the functionally uncharacterized E. coli protein EtpO. PulS and OutS have been shown to interact with and facilitate insertion of secretins into the outer membrane, suggesting a chaperone-like, or piloting function for members of this family. In the pilotin from this four-helix protein from enterohemorrhagic Escherichia coli, the straight helix alpha2, the curved helix alpha3 and the bent helix alpha4 surround the central N-terminal helix alpha1. These helices create a prominent groove, mainly formed by side chains of helices 1,2 and 3 suggesting this groove is important as a binding site.


The actual alignment was detected with superfamily member TIGR01004:

Pssm-ID: 419858  Cd Length: 128  Bit Score: 78.05  E-value: 1.14e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727178801   11 MACLLLSGCQHAAKSAPDAAI----QRDQLSSLVGAGQFLRERCNRADIPANDPLAAAALQAAERKGWApALNRTQLLDA 86
Cdd:TIGR01004  13 VCCVSLSGCQQNTASRSAASVpaneQLEQLASLVAASRYLKVQCNRSDLPDDGTILKTAVNVAVQKGWD-TGRYQSLPQL 91

                          90       100       110
                  ....*....|....*....|....*....|....
gi 727178801   87 GKNVAAQLEADATPLQEKCSGLNRTLAPFLAQLR 120
Cdd:TIGR01004  92 SENLYQGLLKDSTPEATKCSSFNRTLTPFIDALR 125
 
Name Accession Description Interval E-value
PulS_OutS TIGR01004
lipoprotein, PulS/OutS family; This family comprises lipoproteins from four gamma ...
 11-120 1.14e-19

lipoprotein, PulS/OutS family; This family comprises lipoproteins from four gamma proteobacterial species: PulS protein of Klebsiella pneumoniae, the OutS protein of Erwinia chrysanthemi and Pectobacterium chrysanthemi, and the functionally uncharacterized E. coli protein EtpO. PulS and OutS have been shown to interact with and facilitate insertion of secretins into the outer membrane, suggesting a chaperone-like, or piloting function for members of this family. [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273390  Cd Length: 128  Bit Score: 78.05  E-value: 1.14e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727178801   11 MACLLLSGCQHAAKSAPDAAI----QRDQLSSLVGAGQFLRERCNRADIPANDPLAAAALQAAERKGWApALNRTQLLDA 86
Cdd:TIGR01004  13 VCCVSLSGCQQNTASRSAASVpaneQLEQLASLVAASRYLKVQCNRSDLPDDGTILKTAVNVAVQKGWD-TGRYQSLPQL 91

                          90       100       110
                  ....*....|....*....|....*....|....
gi 727178801   87 GKNVAAQLEADATPLQEKCSGLNRTLAPFLAQLR 120
Cdd:TIGR01004  92 SENLYQGLLKDSTPEATKCSSFNRTLTPFIDALR 125
T2SS_PulS_OutS pfam09691
Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins ...
 20-116 5.18e-13

Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins from four gamma proteobacterial species: PulS protein of Klebsiella pneumoniae (P20440), the OutS protein of Erwinia chrysanthemi (Q01567) and Pectobacterium chrysanthemi, and the functionally uncharacterized E. coli protein EtpO. PulS and OutS have been shown to interact with and facilitate insertion of secretins into the outer membrane, suggesting a chaperone-like, or piloting function for members of this family. In the pilotin from this four-helix protein from enterohemorrhagic Escherichia coli, the straight helix alpha2, the curved helix alpha3 and the bent helix alpha4 surround the central N-terminal helix alpha1. These helices create a prominent groove, mainly formed by side chains of helices 1,2 and 3 suggesting this groove is important as a binding site.


Pssm-ID: 401580  Cd Length: 96  Bit Score: 59.94  E-value: 5.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727178801   20 QHAAKSAPDaaiQRDQLSSLVGAGQFLRERCNRADIPANDPLAAAALQAAERKGWAPAlNRTQLLDAGKNVAAQLEADAT 99
Cdd:pfam09691   3 KHENVPANE---QIEQLSSLVAGIKYLKQQCNRSDLPDDATIINVAMIVAKQRGWDTD-NYQSLPQRSERLYQGLLKDST 78

                          90
                  ....*....|....*..
gi 727178801  100 PLQEKCSGLNRTLAPFL 116
Cdd:pfam09691  79 PKGTQCSSFNRTLTPFI 95
 
Name Accession Description Interval E-value
PulS_OutS TIGR01004
lipoprotein, PulS/OutS family; This family comprises lipoproteins from four gamma ...
 11-120 1.14e-19

lipoprotein, PulS/OutS family; This family comprises lipoproteins from four gamma proteobacterial species: PulS protein of Klebsiella pneumoniae, the OutS protein of Erwinia chrysanthemi and Pectobacterium chrysanthemi, and the functionally uncharacterized E. coli protein EtpO. PulS and OutS have been shown to interact with and facilitate insertion of secretins into the outer membrane, suggesting a chaperone-like, or piloting function for members of this family. [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273390  Cd Length: 128  Bit Score: 78.05  E-value: 1.14e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727178801   11 MACLLLSGCQHAAKSAPDAAI----QRDQLSSLVGAGQFLRERCNRADIPANDPLAAAALQAAERKGWApALNRTQLLDA 86
Cdd:TIGR01004  13 VCCVSLSGCQQNTASRSAASVpaneQLEQLASLVAASRYLKVQCNRSDLPDDGTILKTAVNVAVQKGWD-TGRYQSLPQL 91

                          90       100       110
                  ....*....|....*....|....*....|....
gi 727178801   87 GKNVAAQLEADATPLQEKCSGLNRTLAPFLAQLR 120
Cdd:TIGR01004  92 SENLYQGLLKDSTPEATKCSSFNRTLTPFIDALR 125
T2SS_PulS_OutS pfam09691
Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins ...
 20-116 5.18e-13

Type II secretion system pilotin lipoprotein (PulS_OutS); This family comprises lipoproteins from four gamma proteobacterial species: PulS protein of Klebsiella pneumoniae (P20440), the OutS protein of Erwinia chrysanthemi (Q01567) and Pectobacterium chrysanthemi, and the functionally uncharacterized E. coli protein EtpO. PulS and OutS have been shown to interact with and facilitate insertion of secretins into the outer membrane, suggesting a chaperone-like, or piloting function for members of this family. In the pilotin from this four-helix protein from enterohemorrhagic Escherichia coli, the straight helix alpha2, the curved helix alpha3 and the bent helix alpha4 surround the central N-terminal helix alpha1. These helices create a prominent groove, mainly formed by side chains of helices 1,2 and 3 suggesting this groove is important as a binding site.


Pssm-ID: 401580  Cd Length: 96  Bit Score: 59.94  E-value: 5.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727178801   20 QHAAKSAPDaaiQRDQLSSLVGAGQFLRERCNRADIPANDPLAAAALQAAERKGWAPAlNRTQLLDAGKNVAAQLEADAT 99
Cdd:pfam09691   3 KHENVPANE---QIEQLSSLVAGIKYLKQQCNRSDLPDDATIINVAMIVAKQRGWDTD-NYQSLPQRSERLYQGLLKDST 78

                          90
                  ....*....|....*..
gi 727178801  100 PLQEKCSGLNRTLAPFL 116
Cdd:pfam09691  79 PKGTQCSSFNRTLTPFI 95
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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