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Conserved domains on  [gi|727166037|ref|WP_033636451|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Serratia]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10013498)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 8.86e-118

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


:

Pssm-ID: 182406  Cd Length: 157  Bit Score: 328.88  E-value: 8.86e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 727166037  81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDALPAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGALIKA 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
 
Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 8.86e-118

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 328.88  E-value: 8.86e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 727166037  81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDALPAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGALIKA 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-153 9.15e-106

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 298.49  E-value: 9.15e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 727166037  81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDAlPAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGA 153
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-153 2.75e-94

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 269.29  E-value: 2.75e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037    1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 727166037   81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDALpAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGA 153
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-148 4.06e-93

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 266.24  E-value: 4.06e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   3 NIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTTKG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 727166037  83 TPAHSAVSYQAGDYLLFGPETRGLPADILDALPaQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 9.77e-44

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 141.13  E-value: 9.77e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037    1 MLNIVLFEPEIPPNTGNIIRLCANTGFN-LHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQ--RLFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 727166037   78 LTTKGTPAHsAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLEL--CDRLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-157 8.86e-118

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 328.88  E-value: 8.86e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 727166037  81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDALPAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGALIKA 157
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD 157
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-153 9.15e-106

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 298.49  E-value: 9.15e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 727166037  81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDAlPAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGA 153
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-153 2.75e-94

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 269.29  E-value: 2.75e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037    1 MLNIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 727166037   81 KGTPAHSAVSYQAGDYLLFGPETRGLPADILDALpAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAGA 153
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-148 4.06e-93

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 266.24  E-value: 4.06e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   3 NIVLFEPEIPPNTGNIIRLCANTGFNLHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQRLFALTTKG 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 727166037  83 TPAHSAVSYQAGDYLLFGPETRGLPADILDALPaQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 9.77e-44

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 141.13  E-value: 9.77e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037    1 MLNIVLFEPEIPPNTGNIIRLCANTGFN-LHLIEPLGFPWDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQ--RLFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 727166037   78 LTTKGTPAHsAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLEL--CDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-144 3.95e-30

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 106.54  E-value: 3.95e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   4 IVLFEPEIPPNTGNIIRLCANTG-FNLHLIEPLGFPWDDKrLRRAGLDYHEFTRVSRHADYAAFLAAEKPQ--RLFALTT 80
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGvEKIYLVGPTADIFDPK-AKRTSRGTHFSVPYERVDNFEEYLKSLKEEgyPLIGLEL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 727166037  81 KGT---PAHSAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEA 144
Cdd:cd18082   81 TDGsipLSEFKFKKPKKIALIFGNERSGLSPELLAL--CDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 2-143 4.95e-16

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 70.22  E-value: 4.95e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   2 LNIVLFEPEIPPNTGNIIRLCANTGF-NLHLIEPLGFPWDDKRLRRA--GLDYHE--------------------FTRVS 58
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLsDLRLVNPRCDDPEEAARALAsgAEDVLEnakvydtleealadcdlvvaTTARP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  59 RHADYAAFLAAEKPQRLFALTTKGTPAhsavsyqagdyLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVS 138
Cdd:cd18093   81 RGLRRPLLTPRELAELLKEHAAGGKVA-----------LVFGREDSGLTNEELAL--CDLLVTIPTNPEYPSLNLAQAVA 147


                 ....*
gi 727166037 139 VVVYE 143
Cdd:cd18093  148 IVLYE 152
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 14-148 1.67e-13

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 64.03  E-value: 1.67e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  14 NTGNIIRLCANTGF-NLHLIEPL-GFPWDDKRLRRAgldyHEFTRVSRHADYAAFLAAEKPQ--RLFALTtkgtPAHSAV 89
Cdd:cd18092   29 NASAVLRTCDAFGVqDVHVIEPKnKFKKNRGVAMGA----DKWLDVHRHDSTEECLAALKEQgyRIVATS----LSPDAV 100

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 727166037  90 SYQAGDY-----LLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQL 148
Cdd:cd18092  101 PLDELDYtrptaLVFGNEKDGVSEEALAA--ADERVYIPMYGFVESLNVSVAAAIILYELQRQR 162
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 74-147 3.28e-12

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 60.29  E-value: 3.28e-12
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 727166037  74 RLFALTTKGTPAHSAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQ 147
Cdd:cd18104   74 RILATTPKAEKLYYEADLRGPIAIVVGSESEGLSEFWLKA--ADERVRIPMAGQADSLNVSVAAAILLYEALRQ 145
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 4-148 4.76e-12

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 61.29  E-value: 4.76e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   4 IVLFEPEIPPNTGNIIRLCANTGF-NLHLIEPLGFPwDDKRLRRAG-----LD---YHE--------------FTRVSRH 60
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKNFGLsDLRLVNPRGWP-DEEARAMASgaddvLEnarVVDtleealadcdlvvgTTARPRE 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  61 ADYAAFLAAEKPQRLFALTTKGTPAhsavsyqagdyLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVV 140
Cdd:COG0565   85 LRKPVLTPREAAEELLARAAGGKVA-----------LVFGRERSGLTNEELAL--CDALVTIPTNPEYPSLNLAQAVQVV 151


                 ....*...
gi 727166037 141 VYEAWRQL 148
Cdd:COG0565  152 AYELRKAA 159
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 4-144 1.11e-11

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 58.94  E-value: 1.11e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037   4 IVLFE-PEIPPNTGNIIRLCAntGFNLHLIEPLGF---PWDDKRLRRA-GLDYH-EFTRVSRHADYAAFLAAEKPqRLFA 77
Cdd:cd18107    2 VVLFDrPTSPGNLGTIIRSAD--ALGAHGLVVTGHaadVYDPKTIRAStGSLFSlPIVRVPSNRELEDWVEARKA-RGTP 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 727166037  78 LTTKGTPAHSAVSYQAGDY-----LLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEA 144
Cdd:cd18107   79 LQLVGTDEHGDHQIADHDFtrptlLLIGNETAGLSAAWREL--CDETVSIPMAGSASSLNAACAASIFLYEA 148
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 52-147 1.29e-10

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 57.90  E-value: 1.29e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  52 HEFTRVSRHADYAAFLAA--EKPQRLFALTtkgtpAHSAVSYQAGDY-----LLFGPETRGLPADILDAlpAQQKIRIPM 124
Cdd:COG0566  166 LFHVPVARVTNLADALEElkEAGFWVVGTD-----LDGEASLYEVDLpgpvaLVLGNEGKGLSRLVLEA--CDELVRIPM 238

                         90       100
                 ....*....|....*....|...
gi 727166037 125 QAQSRSMNLSNAVSVVVYEAWRQ 147
Cdd:COG0566  239 AGGVESLNVAVAAAILLYEAARQ 261
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 14-144 1.73e-09

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 52.95  E-value: 1.73e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  14 NTGNIIRLCANTGFN-LHLIEPLGFPwDDKRLRRAGLDYHEFTRVSRHADYAAFLAAEKPQ--RLFAL-TTKGTPAHSAV 89
Cdd:cd18097   13 NVGSIFRTADAAGVEkIYLCGYTPTP-PHPKVAKTALGAEESVPWEHFEDTLEAVRALKARgyTIIALeTTENSVPLHEA 91

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 727166037  90 SYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEA 144
Cdd:cd18097   92 RLPRPLALVLGNEVRGVSPEVLAL--CDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 12-144 8.02e-09

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 51.27  E-value: 8.02e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  12 PPNTGNIIRLCANTGFNlHLIepLG----FPWDDKRLRRA-GldYHEFTRVSRHADYAAFLAA--EKPQRLFALTTKGTP 84
Cdd:cd18095   11 PGNLGTIIRTAAAFGVD-GVI--LSkgtvDPYNPKVVRASmG--ALFRLPIIRVDDLEELLEElkEAGYKVVAADLDGGS 85

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  85 AHSAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEA 144
Cdd:cd18095   86 SLYDLDLPKPVALVLGNEGNGLSDELLEL--CDKRVTIPMSGGVESLNVAVAAAILLYEL 143
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 12-152 5.80e-08

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 49.99  E-value: 5.80e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  12 PPNTGNIIRLCANTGFN-LHLIeplgfpWDDKRLRRAG---LDYHEFTRVSRHADYAAFLAAEKPQRLFALTTkgTPAHS 87
Cdd:PRK11081  30 PHNVSAIIRTADAVGVHeVHAV------WPGSRMRTMGstaAGSNSWVQVKTHRTIGDAVAHLKGQGMQILAT--HLSDT 101

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  88 AVSYQAGDY-----LLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEAWRQLDYAG 152
Cdd:PRK11081 102 AVDFREIDYtrptcILMGQEKTGISQEALAL--ADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAG 169
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 1-143 1.81e-07

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 48.55  E-value: 1.81e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037    1 MLNIVLFEPEIPPNTGNIIRLCANTGF-NLHLIEPLGFPWDDKRLRRAGL-DYHEFTRVSRhaDYAAFLAaeKPQRLFAL 78
Cdd:TIGR00050   3 NIRIVLVEPSHPGNIGSIARAMKNMGLtELRLVNPRVKLEEEAYALAAGArDILDNAKVVD--TLDEALD--DCDFVVGT 78

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 727166037   79 TTKG--------TP---AHSAVSYQAGDYLLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYE 143
Cdd:TIGR00050  79 SARSrnlqrpmlTPrelAPKLVAEKGKVALVFGREDSGLTNEELQK--CHVLVSIPTSEEYPSLNLSHAVAVILYE 152
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 98-142 1.37e-05

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 42.25  E-value: 1.37e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 727166037  98 LFGPETRGLPADILDAlpAQQKIRIPMQaqsRSMNLSNAVSVVVY 142
Cdd:cd18098   97 LLGPEDGGLPQEVLAR--CHHVVYLPTR---GSLNVAVAGSIVLY 136
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 12-143 5.43e-05

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 40.98  E-value: 5.43e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  12 PPNTGNIIRLCantgfnlhliEPLGFpwddKRL---RRAGLDYHEFTRVSRHADY------------AAFLAAEKPQ--R 74
Cdd:cd18091   11 PPNLGGLCRTC----------EIFGV----SKLvlpNLAVVEDKQFKALSVSAEKwlpieevkpedlLPYLKEKKQEgyT 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 727166037  75 LFAL--TTKGTPAHSavsYQAGDY--LLFGPETRGLPADILDALpaQQKIRIPMQAQSRSMNLSNAVSVVVYE 143
Cdd:cd18091   77 IVGLeqTANSVSLHD---FQFPKKtvLVLGNEKEGIPAELLSLL--DVCVEIPQLGVIRSLNVHVSGAIAIYE 144
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 97-144 5.84e-04

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 37.75  E-value: 5.84e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 727166037  97 LLFGPETRGLPADILDAlpAQQKIRIPMQAQSRSMNLSNAVSVVVYEA 144
Cdd:cd18103   98 LVLGSEGKGLRRLVLKN--CDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 41-143 3.31e-03

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 35.92  E-value: 3.31e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727166037  41 DKRLRRAGLDYhEFTRVSRHADYAAFLAAEKPQ--RLFALTTKG-TPAHSAVSYQAGDYLLFGPETRGLPADILDAlpAQ 117
Cdd:cd18108   41 DRRLLRASRGY-VFSLPVVLADREEAVAFLRDNdiPLMTLDADGdVPVKELGDRPDRLALVFGSEKGGPSGLFEEA--AA 117

                         90       100
                 ....*....|....*....|....*.
gi 727166037 118 QKIRIPMQAQSRSMNLSNAVSVVVYE 143
Cdd:cd18108  118 GTVSIPMSSSTESLNVSVSAGIALHE 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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