|
Name |
Accession |
Description |
Interval |
E-value |
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
1-421 |
0e+00 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 830.18 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 1 MFALADVNSFYASCEKVFRPDLRDKPVVVLSNNDGCVIARSKEAKRLGIKMGIPWFQLKAMQFPEKILTFSSNYELYASL 80
Cdd:PRK03609 1 MFALCDVNSFYASCETVFRPDLRGKPVVVLSNNDGCVIARSAEAKALGIKMGDPWFKQKDLFRRCGVVCFSSNYELYADM 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 81 SNRVMAHLEELAPRVEQYSIDEMFLDIRGIDGCTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKEW-PQF 159
Cdd:PRK03609 81 SNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWqRQT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 160 GGVLALTtdNLRRTEKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEACISLE 239
Cdd:PRK03609 161 GGVVDLS--NLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRGEPCLSLE 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 240 EAPPAKQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVNEAYYGNVASEKLLVPTRD 319
Cdd:PRK03609 239 EFAPTKQEIVCSRSFGERITDYESMRQAICSYAARAAEKLRGEHQYCRFISTFVKTSPFALNEPYYGNSASVKLLTPTQD 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 320 TRDIINAAVKALDRIWLDGHRYAKAGVMLNDFTPTGVAQLNLFDDAKPQERSEELMKALDGIN-HSGLGRIWFAGRGIAP 398
Cdd:PRK03609 319 SRDIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMKVLDTLNaKSGKGTLYFAGQGIAQ 398
|
410 420
....*....|....*....|...
gi 695723888 399 EWQMKREMLSPAYTTRWSDIPRA 421
Cdd:PRK03609 399 QWQMKREMLSPRYTTRWSDLLRV 421
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
3-350 |
8.58e-178 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 499.38 E-value: 8.58e-178
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 3 ALADVNSFYASCEKVFRPDLRDKPVVVLSNNDGCVIARSKEAKRLGIKMGIPWFQLKamQFPEKILT--FSSNYELYASL 80
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGCVIARSPEAKALGIKMGSPYFKVP--DLLERHGVavFSSNYALYGDM 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 81 SNRVMAHLEELAPRVEQYSIDEMFLDIRGIDGCTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKEWPQFG 160
Cdd:cd01700 79 SRRIMSILERFSPDVEVYSIDESFLDLTGSLRFGDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 161 GVLALTTDNLRRteKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEACISLEE 240
Cdd:cd01700 159 GVVDLTDEEVRD--KLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 241 APPAKQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVNEAYYGnvASEKLLVPTRDT 320
Cdd:cd01700 237 YPPPKKSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSGFSRQPKYYS--ATNTLPYPTNDT 314
|
330 340 350
....*....|....*....|....*....|
gi 695723888 321 RDIINAAVKALDRIWLDGHRYAKAGVMLND 350
Cdd:cd01700 315 REIVKAALRLLYAIYRPGYAYRKAGVMLSD 344
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
1-348 |
2.19e-129 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 376.41 E-value: 2.19e-129
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 1 MFALADVNSFYASCEKVFRPDLRDKPVVVLS-NNDGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEkILTFSSNYELYAS 79
Cdd:COG0389 2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGdNNRGVVAAASYEARAFGVRSGMPLFQARRL-CPD-LVVLPPDFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 80 LSNRVMAHLEELAPRVEQYSIDEMFLDIRGIDGC-TDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKEwpq 158
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLfGSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKP--- 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 159 fGGVLALTTDNLRRtekLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEACISL 238
Cdd:COG0389 157 -DGLTVIPPGEVAA---FLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPV 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 239 EEAPPAKqQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVNEayygnvASEKLLVPTR 318
Cdd:COG0389 233 EPRRPRK-SIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTT------RSRTLPEPTD 305
|
330 340 350
....*....|....*....|....*....|
gi 695723888 319 DTRDIINAAVKALDRIWLDGHRYAKAGVML 348
Cdd:COG0389 306 DTAELLRAARELLERIYRPGRPVRLLGVRL 335
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
6-337 |
4.38e-59 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 195.82 E-value: 4.38e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNNDGCVIAR-SKEAKRLGIKMGIPWFQlkAMQF-PEKILtFSSNYELYASLSNR 83
Cdd:cd03586 4 DMDAFYASVEQRDNPELKGKPVAVGGSSDRGVVSTaSYEARKFGVRSAMPIFQ--AKKLcPNLIF-VPPRFDKYREVSRQ 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAPRVEQYSIDEMFLDIRGIDGCTDF-EDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPQfgGV 162
Cdd:cd03586 81 IMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSaTEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNK--PN--GL 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 163 LALTTDNLRrtEKLLSLqPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEACISLEEAP 242
Cdd:cd03586 157 TVIPPEDVE--EFLAPL-PVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDR 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 243 PAKqQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVneayygNVASEKLLVPTRDTRD 322
Cdd:cd03586 234 ERK-SIGVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFST------RTRSRTLPEPTDDAED 306
|
330
....*....|....*
gi 695723888 323 IINAAVKALDRIWLD 337
Cdd:cd03586 307 IYELALELLEELLDG 321
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
4-383 |
1.06e-53 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 184.05 E-value: 1.06e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 4 LADVNSFYASCEKVFRPDLRDKPVVVL---SNNDGCVIARSKEAKRLGIKMGIPWFQLKAmQFPEKILtFSSNYELYASL 80
Cdd:PRK03103 7 LVDMQSFYASVEKAANPELKGRPVIVSgdpERRSGVVLAACPLAKAYGVKTAERLWEAQQ-KCPDLVV-VKPRMQRYIDV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 81 SNRVMAHLEELAPRVEQYSIDEMFLDIRgidGCTDF----EDFARQLREHVLIGTGLTIGVGMGPTKTLAKSA--QWASK 154
Cdd:PRK03103 85 SLQITRILEDFTDLVEPFSIDEQFLDVT---GSQKLfgspLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMAcdNFAKK 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 155 EwPQfgGVLALTTDNLRRteKLLSLqPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGea 234
Cdd:PRK03103 162 N-PD--GLFTLDKEDVPA--DLWPL-PVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANG-- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 235 cisLEEAPPAKQQIVCSRSFGERIT------TYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFavnEAYYGNV 308
Cdd:PRK03103 234 ---IDYSPVTPHSLDRQKAIGHQMTlprdyrGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRGADF---DWPTGFS 307
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 695723888 309 ASEKLLVPTRDTRDIINAAVKALDRIWlDGHRYAKAGVMLNDFTPTGVAQLNLFDDakpQERSEELMKALDGINH 383
Cdd:PRK03103 308 RQMTLPEPTNLAMEVYEAACKLFHRHW-DGKPVRRVGVTLSNLVSDDVWQLSLFGD---RERKRSLGYVMDDIKN 378
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
6-348 |
2.22e-51 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 176.01 E-value: 2.22e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNND--GCVIARSKEAKRLGIKMGIPWFQLKAMqFPEKILTfSSNYELYASLSNR 83
Cdd:cd00424 4 DFDNFFASVEQLARPELKGRPVVVVPFNSdsTCVIACSYEARKYGVKRGMPVREARKM-CPNLILV-PARLDLYRRLSER 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAPRVEQYSIDEMFLDIRGID-GCTDFEDFARQLREHVLIGTG-LTIGVGMGPTKTLAKSAQWASKEwpqfGG 161
Cdd:cd00424 82 LLSELEEVAPLVEVASIDELFLDLTGSArLLGLGSEVALRIKRHIAEQLGgITASIGIASNKLLAKLAAKYAKP----DG 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 162 VLALTTDNLRRtekLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNP-AFIRKNFTVVLERTVRELNGEACISLEE 240
Cdd:cd00424 158 LTILDPEDLPG---FLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAASPdALLALWGGVSGERLWYALRGIDDEPLSP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 241 APPaKQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVNEAYYGNVASEKLLVPTRDt 320
Cdd:cd00424 235 PRP-RKSFSHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDGRGATRLRLWLRTVDGRWSGHADIPSRSAPRPISTED- 312
|
330 340
....*....|....*....|....*...
gi 695723888 321 RDIINAAVKALDRIWLDGHRYAKAGVML 348
Cdd:cd00424 313 GELLHALDKLWRALLDDKGPRRLRRLGV 340
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
6-149 |
1.84e-41 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 143.87 E-value: 1.84e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNN-DGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEkILTFSSNYELYASLSNRV 84
Cdd:pfam00817 2 DMDAFFASVELLRDPELKGKPVAVGGGNgRGIVAAASYEARKYGVRSGMPVFEAKKL-CPN-LIVVPPDLELYRRASRKI 79
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 695723888 85 MAHLEEL-APRVEQYSIDEMFLDIRGIDGC-TDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSA 149
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEKLfGAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKLA 146
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
6-382 |
1.42e-38 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 143.63 E-value: 1.42e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSN---NDGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEKILTfSSNYELYASLSN 82
Cdd:PRK01810 11 DMNSFFASVEIAYDPSLQGKPLAVAGNekeRKGIIVTCSYEARAYGIRTTMPLWEAKRL-CPQLIVR-RPNFDRYREASR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 83 RVMAHLEELAPRVEQYSIDEMFLDIRGIDGCTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPqfggv 162
Cdd:PRK01810 89 QMFQILSEFTPLVQPVSIDEGYLDITDCYALGSPLEIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKK--P----- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 163 LALTTDNLRRTEKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEacislEEAP 242
Cdd:PRK01810 162 LGITVLRKRDVPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGI-----DDRP 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 243 PAKQQIVCSRSFG------ERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAvneayygNVA-SEKLLV 315
Cdd:PRK01810 237 VDPEAIYQFKSVGnsttlsHDMDEEKELLDVLRRLSKSVSKRLQKKTVVSYNVQIMIRYHDRR-------TITrSKTLKN 309
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 695723888 316 PTRDTRDIINAAVKALDRIWlDGHRYAKAGVMLND-FTPTG-VAQLNLF---DDAKpqerSEELMKALDGIN 382
Cdd:PRK01810 310 PIWEKRDIFQAASRLFKQHW-NGDPVRLLGVTATDlEWKTEaVKQLDLFsfeEDAK----EEPLLAVIDQIN 376
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
6-337 |
3.81e-37 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 138.23 E-value: 3.81e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNND-----GCVIARSKEAKRLGIKMGIPwFQLKAMQFPEKILtFSSNYELYASL 80
Cdd:PRK03352 11 DLDQFIAAVELLRRPELAGLPVIVGGNGDpteprKVVTCASYEARAFGVRAGMP-LRTAARRCPDAVF-LPSDPAAYDAA 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 81 SNRVMAHLEELAPRVEQYSIDEMFLDIRgidgCTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKEwpqfG 160
Cdd:PRK03352 89 SEEVMATLRDLGVPVEVWGWDEAFLGVD----TDDPEALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKP----A 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 161 GVLALTTDNLRrteKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNF-------TVVLERTVrelnGE 233
Cdd:PRK03352 161 GVFRLTDANWM---AVMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFgpttgpwLLLLARGG----GD 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 234 ACISLEEAPPAkqqivcSRS----FGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFavneayYGNVA 309
Cdd:PRK03352 234 TEVSAEPWVPR------SRSrevtFPQDLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTATF------YTRTK 301
|
330 340
....*....|....*....|....*...
gi 695723888 310 SEKLLVPTRDTRDIINAAVKALDRIWLD 337
Cdd:PRK03352 302 IRKLPEPTTDPDVIEAAALDVLDRFELD 329
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
6-333 |
3.79e-36 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 137.76 E-value: 3.79e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVV--LSNNdGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEKILTFSSNYELYASLSNR 83
Cdd:PRK03348 11 DMDAFFASVEQLTRPTLRGRPVLVggLGGR-GVVAGASYEARVFGARSAMPMHQARRL-VGNGAVVLPPRFVVYRAASRR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAPRVEQYSIDEMFLDIRGIDGCT--DFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPQfgG 161
Cdd:PRK03348 89 VFDTLRELSPVVEQLSFDEAFVEPAELAGASaeEVEAFAERLRARVREETGLPASVGAGSGKQIAKIASGLAK--PD--G 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 162 VLALTTDnlrRTEKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKnftvVLERTVrelnGEACISL--- 238
Cdd:PRK03348 165 IRVVPPG---EERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSEAEVAN----LLGATV----GPALHRLarg 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 239 ------EEAPPAKqQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVneayygNVASEK 312
Cdd:PRK03348 234 iddrpvAERAEAK-QISAESTFAVDLTTRAQLREAIERIAEHAHRRLLKDGRGARTVTVKLRKSDFST------LTRSAT 306
|
330 340
....*....|....*....|.
gi 695723888 313 LLVPTRDTRDIINAAVKALDR 333
Cdd:PRK03348 307 LPYATDDAAVLAATARRLLLD 327
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
6-362 |
2.03e-33 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 128.30 E-value: 2.03e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVV--LSNNdGCVIARSKEAKRLGIKMGIPWFQLKAmQFPEKILtFSSNYELYASLSNR 83
Cdd:PRK14133 9 DMDAFFASVEQMDNPKLKGKPVIVggISER-GVVSTCSYEARKYGVHSAMPVFMAKK-RCPHGIF-LPVRHERYKEVSKN 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAPRVEQYSIDEMFLDIRGIDgcTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQwaskEWPQFGGVL 163
Cdd:PRK14133 86 IFKILYEVTPIVEPVSIDEAYLDITNIK--EEPIKIAKYIKKKVKKETGLTLSVGISYNKFLAKLAS----DWNKPDGIK 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 164 ALTTDNLrrTEKLLSLqPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIR----KNFTVVLERtVRELNGEACISLE 239
Cdd:PRK14133 160 IITEDMI--PDILKPL-PISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIeyfgKFGVEIYER-IRGIDYREVEVSR 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 240 EappaKQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVneayygNVASEKLLVPTRD 319
Cdd:PRK14133 236 E----RKSIGKETTLKKDTKDKEELKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQT------HTKSKTLNDYIRD 305
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 695723888 320 TRDIINAAVKALDRIWLDghRYAK-AGVMLNDFTPTGVAQLNLF 362
Cdd:PRK14133 306 KEEIYNVACEILEHINIK--EPIRlIGLSVSNLSENKIEQLSFL 347
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
5-381 |
1.39e-30 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 121.25 E-value: 1.39e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 5 ADVNSFYASCEKVFRPDLRDKPVVVlsnNDGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEKIlTFSSNYELYASLSNRV 84
Cdd:PRK03858 9 ADLDSFYASVEQRDDPALRGRPVIV---GGGVVLAASYEAKAYGVRTAMGGRQARRL-CPQAV-VVPPRMSAYSRASKAV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 85 MAHLEELAPRVEQYSIDEMFLDIRG---IDGCTdfEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPQfgG 161
Cdd:PRK03858 84 FEVFRDTTPLVEGLSIDEAFLDVGGlrrISGTP--VQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAK--PD--G 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 162 VLALTTDnlRRTEKLLSLqPVEEIWGVGRRIGKKLNTYGVTTALQLARMNpafirknftvvlERTVRELNGEA------C 235
Cdd:PRK03858 158 LLVVPPD--RELAFLHPL-PVRRLWGVGPVTAAKLRAHGITTVGDVAELP------------ESALVSLLGPAagrhlhA 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 236 ISLEEAPPA------KQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAvneayyGNVA 309
Cdd:PRK03858 223 LAHNRDPRRvetgrrRRSVGAQRALGRGPNSPAEVDAVVVALVDRVARRMRAAGRTGRTVVLRLRFDDFT------RATR 296
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 695723888 310 SEKLLVPTRDTRDIINAAVKALDRIW-LDGHRYAK-AGVMLNDFTPTGVAQLNL-FDDAKPqerSEELMKALDGI 381
Cdd:PRK03858 297 SHTLPRPTASTATLLAAARDLVAAAApLIAERGLTlVGFAVSNLDDDGAQQLELpFGLRRP---GSALDAALDAV 368
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
245-355 |
1.74e-27 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 104.95 E-value: 1.74e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 245 KQQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAvneayyGNVASEKLLVPTRDTRDII 324
Cdd:pfam11799 1 RKSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFR------TITRSVTLPSPTDDTDEIY 74
|
90 100 110
....*....|....*....|....*....|.
gi 695723888 325 NAAVKALDRIWlDGHRYAKAGVMLNDFTPTG 355
Cdd:pfam11799 75 RAALRLLRRLY-RGRPVRLLGVSLSNLVPEG 104
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
6-381 |
4.19e-27 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 111.95 E-value: 4.19e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNNDG-----CVIARSKeakrlGIKMGIPWFQ-LKAMqfPEKILtFSSNYELYAS 79
Cdd:PRK02794 42 DCDAFYASVEKRDNPELRDKPVIIGGGKRGvvstaCYIARIH-----GVRSAMPMFKaLKLC--PDAVV-IKPDMEKYVR 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 80 LSNRVMAHLEELAPRVEQYSIDEMFLDIRGidgcTD----------FEDFARQLREHVligtGLTIGVGMGPTKTLAKSA 149
Cdd:PRK02794 114 VGREVRAMMQALTPLVEPLSIDEAFLDLSG----TErlhgappavvLARFARRVEREI----GITVSVGLSYNKFLAKIA 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 150 QWASKewPQFGGVLALTtdnlrRTEKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRE 229
Cdd:PRK02794 186 SDLDK--PRGFSVIGRA-----EALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRL 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 230 LNGEACISLEEAPPAKqQIVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPFAVneayygNVA 309
Cdd:PRK02794 259 ARGIDDRKVSPDREAK-SVSAETTFETDLSDFEDLEPILWRLSEKVSRRLKAAGLAGRTVTLKLKTADFRL------RTR 331
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 695723888 310 SEKLLVPTRdTRDIINAAVKALDRIWLDGHRYAKAGVMLNDFTPTGVAQL-NLFDDAKPQERSEElmKALDGI 381
Cdd:PRK02794 332 RRTLEDPTQ-LADRIFRTARELLEKETDGTAFRLIGIGVSDLSPADEADPpDLLDPQATRRAAAE--RAIDAL 401
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
1-201 |
8.46e-24 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 101.40 E-value: 8.46e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 1 MFALADVNSFYASCEKVFRPDLRDKPVVVL-----SNNDGCVIARSKEAKRLGIKMGIPWFqlKAMQFPEKILTFSSNYE 75
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVCvysgrFEDSGAVATANYEARKLGIKAGMPIV--EAKKILPNAVYLPMRKE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 76 LYASLSNRVMAHLEELAPRVEQYSIDEMFLDIRgiDGCTDFED---FARQLREHVLIGTGLTIGVGMGPTKTLAKSAqwA 152
Cdd:PRK01216 80 VYQQVSNRIMKLLREYSEKIEIASIDEAYLDIS--DKVKNYQDaynLGLEIKNKILEKEKITVTVGISKNKVFAKIA--A 155
|
170 180 190 200
....*....|....*....|....*....|....*....|....*....
gi 695723888 153 SKEWPQFGGVLALTtdnlrRTEKLLSLQPVEEIWGVGRRIGKKLNTYGV 201
Cdd:PRK01216 156 DMAKPNGIKVIDDE-----EVKRFINELDIADIPGIGDITAEKLKKLGV 199
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
6-295 |
2.17e-21 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 95.46 E-value: 2.17e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSN-NDGCVIAR-SKEAKRLGIKMGIpWFQlKAMQFPEKILTFSSNYELYASLSNR 83
Cdd:cd01701 53 DFDCFFVSVSIRNRPDLKGKPVAVCHGkGPNSEIAScNYEARSYGIKNGM-WVG-QAKKLCPQLVTLPYDFEAYEEVSLT 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAPRVEQYSIDEMFLDIRGIDGCT--DFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPqfGG 161
Cdd:cd01701 131 FYEILASYTDNIEAVSCDEALIDITSLLEETyeLPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAK--P--DG 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 162 VLALTTDNLrrtEKLLSLQPVEEIWGVGRRIGKKL-NTYGVTTALQLARMNPAFIrknftvvLERTVRELNGE----ACI 236
Cdd:cd01701 207 QYHLSAEKV---EEFLSQLKVGDLPGVGSSLAEKLvKLFGDTCGGLELRSKTKEK-------LQKVLGPKTGEklydYCR 276
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 695723888 237 SLEEAPPAKQQ----IVCSRSFGERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKT 295
Cdd:cd01701 277 GIDDRPVTGEKerksVSAEINYGIRFTNVDDVEQFLQRLSEELSKRLEESNVTGRQITLKLMK 339
|
|
| DUF4113 |
pfam13438 |
Domain of unknown function (DUF4113); Although the function is not known this domain occurs ... |
374-421 |
3.13e-20 |
|
Domain of unknown function (DUF4113); Although the function is not known this domain occurs almost invariably at the very C-terminus of the IMS family DNA-polymerase repair proteins, IMS, pfam00817.
Pssm-ID: 463876 [Multi-domain] Cd Length: 49 Bit Score: 83.30 E-value: 3.13e-20
10 20 30 40
....*....|....*....|....*....|....*....|....*....
gi 695723888 374 LMKALDGINHS-GLGRIWFAGRGIAPEWQMKREMLSPAYTTRWSDIPRA 421
Cdd:pfam13438 1 LMAALDAINRRyGRGTVRLAAQGLKRDWKMRREHLSPRYTTRWDELPVV 49
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
10-219 |
6.60e-13 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 69.38 E-value: 6.60e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 10 FYASCEKVFRPDLRDKPVVVLSNND--GCVIARSKEAKRLGIKMGIPWFQlkAMQF-PEKILTfSSNYELYASLSNRVMA 86
Cdd:PRK02406 4 FYAAVEMRDNPELRGKPVAVGGSPGrrGVISTCNYEARKFGVRSAMPTAQ--ALKLcPDLIFV-PGRFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 87 HLEELAPRVEQYSIDEMFLDI----RGIDGCTdfeDFARQLREHVLIGTGLTIGVGMGPTKTLAKSaqwAS--------- 153
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVtdnkLCIGSAT---LIAQEIRQDIFEELGLTASAGVAPNKFLAKI---ASdwnkpnglf 154
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 154 ----KEWPQFggVLALttdnlrrtekllslqPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNF 219
Cdd:PRK02406 155 vitpEEVDAF--LATL---------------PVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHF 207
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
6-298 |
1.78e-11 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 65.81 E-value: 1.78e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVlsnndGCVIARSKE---AKRLGIKMGIPWFQlkAMQFPEKILTFSSNYELYASLSN 82
Cdd:PTZ00205 139 DMDMFYAAVEIKKHPEYAAIPLAI-----GTMTMLQTAnyvARGRGIRQGMPGFL--ALKICPNLLILPPDFDAYNEESN 211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 83 RVMAHLEELAPRVEQYSIDEMFLD----IRGIDGCTDFEDFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKewPQ 158
Cdd:PTZ00205 212 TVRRIVAEYDPNYISFGLDELTLEvsayIERFEGTKTAEDVASELRVRVFGETKLTASAGIGPTAALAKIASNINK--PN 289
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 159 FGGVLAL-TTDNLRRTEKLLSLQPVEeiwGVGRRIGKKLNTYGVTTALQL--ARMNPAFIRKNftvvleRTVRELNGeAC 235
Cdd:PTZ00205 290 GQHDLNLhTRGDVMTYVRDLGLRSVP---GVGKVTEALLKGLGITTLSDIynRRVELCYILHN------NLFRFLLG-AS 359
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 695723888 236 ISLEEAPPA----------------KQQIVCSRSFgERITTYDALRQAICQYSERAAEKLRGEHQFCRHISVFIKTSPF 298
Cdd:PTZ00205 360 IGIMQWPDAataantencegatggqRKAISSERSF-TTPRTKEGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWASY 437
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
3-338 |
8.85e-10 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 60.02 E-value: 8.85e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 3 ALADVNSFYASCEKVFRPDLRDKPVVVLSNNDgcVIARSKEAKRLGIK--MGIP----------------WFQLKAMQFP 64
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS--IIAVSYAARAFGVTrfMTIDeakkkcpdlilahvatYKKGEDEADY 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 65 EKIL---TFSSNYELYASLSNRVMAHLEELAPRVEQYSIDEMFLDIRGIdgctdfedFARQLREHVLIGTGLTIGVGMGP 141
Cdd:cd01702 79 HENPspaRHKVSLDPYRRASRKILNILKRFGDVVEKASIDEAYLDLGSR--------IVEEIRQQVYDELGYTCSAGIAH 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 142 TKTLAKSAQWASKEWPQfggvlalTTDNLRRTEKLLSLQPVEEIWGVGRRIGKKL-NTYGVTTALQLARMN--PAFIRKN 218
Cdd:cd01702 151 NKMLAKLASGMNKPNAQ-------TILRNDAVASFLSSLPITSIRGLGGKLGEEIiDLLGLPTEGDVAGFRssESDLQEH 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 219 FTVVLERTV-RELNGEACISLEEAPPAKqQIVCSRSFGERITTydALRQAICQYSERAAE------KLRGE-----HQFC 286
Cdd:cd01702 224 FGEKLGEWLyNLLRGIDHEPVKPRPLPK-SMGSSKNFPGKTAL--STEDVQHWLLVLASElnsrleDDRYEnnrrpKTLV 300
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 695723888 287 RHISVFIKTSpfavneayygnVASEKLLVPTRDTRDIINAAVKALDRIWLDG 338
Cdd:cd01702 301 LSLRQRGDGV-----------RRSRSCALPRYDAQKIVKDAFKLIKAINEEG 341
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
19-259 |
2.31e-09 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 58.55 E-value: 2.31e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 19 RPDLRDKPVVVLS-NNDGCVIARSKEAKRLGIKMGIPWFQLKAMqFPEkILTFSSNYELYASLSNRVMAHLEELAPRVEQ 97
Cdd:cd03468 17 RPADDEAPLAVVErKKAGRILACNAAARAAGVRPGMPLAEALAL-CPN-LQVVEYDPEADARALQELALWLLRFTPLVAL 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 98 YSIDEMFLDIRGIDGCTDFED-FARQLREHVLIgTGLTIGVGMGPTKTLAKsaqWASKewpqFGGVLALTTDNLRRTEKL 176
Cdd:cd03468 95 DGPDGLLLDVTGCLHLFGGEDaLAASLRAALAT-LGLSARAGIADTPGAAW---LLAR----AGGGRGVLRREALAAALV 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 177 LSLQPVEEIWGVGRRIGKKLNTYGVTTALQLARMNPAFIRKNFTVVLERTVRELNGEA--CISLEEAPPAKQQIVcSRSF 254
Cdd:cd03468 167 LLAPLPVAALRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQAYGRDpePLLFSPPPPAFDFRL-ELQL 245
|
....*
gi 695723888 255 GERIT 259
Cdd:cd03468 246 EEPIA 250
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
6-208 |
3.02e-08 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 55.17 E-value: 3.02e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 6 DVNSFYASCEKVFRPDLRDKPVVVLSNNdgCVIARSKEAKRLGIK--MGIPWFQLKAmqfPEKILTFSSNYELYASLSNR 83
Cdd:cd01703 4 DLDCFYAQVEEIRDPSLKSKPLGIQQKY--IVVTCNYEARRLGVKklMSIKDAKEIC---PDLVLVNGEDLTPFRDMSKK 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695723888 84 VMAHLEELAP--RVEQYSIDEMFLDIrgidgcTDFE-----DFARQLREHVLIGTGLTIGVGMGPTKTLAKSAQWASKEW 156
Cdd:cd01703 79 VYRLLRSYSWndRVERLGFDENFMDV------TEMRllvasHIAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKPN 152
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|..
gi 695723888 157 PQfGGVLALTTDNLrrtEKLLSLQPVEEIWGVGRRIGKKLNTYGVTTALQLA 208
Cdd:cd01703 153 QQ-TTLLPPSCADL---MDFMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQ 200
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
181-201 |
1.31e-03 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 36.22 E-value: 1.31e-03
|
| H3TH |
COG3743 |
Predicted 5' DNA nuclease, flap endonuclease-1-like, helix-3-turn-helix (H3TH) domain ... |
185-217 |
2.56e-03 |
|
Predicted 5' DNA nuclease, flap endonuclease-1-like, helix-3-turn-helix (H3TH) domain [Replication, recombination and repair];
Pssm-ID: 442957 [Multi-domain] Cd Length: 92 Bit Score: 36.83 E-value: 2.56e-03
10 20 30
....*....|....*....|....*....|...
gi 695723888 185 IWGVGRRIGKKLNTYGVTTALQLARMNPAFIRK 217
Cdd:COG3743 28 IKGIGPKIEKKLNELGIFTFAQIAAWTPEDIAW 60
|
|
| |
