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Conserved domains on  [gi|653678460|ref|WP_027661233|]
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non-ribosomal peptide synthetase [Salinispora fenicalii]

Protein Classification

non-ribosomal peptide synthetase( domain architecture ID 1002729)

non-ribosomal peptide synthetase is a modular multidomain enzyme that acts as an assembly line to catalyze the biosynthesis of complex natural products; may contain C-terminal alpha/beta hydrolase domain

EC:  6.2.1.-
Gene Ontology:  GO:0019184|GO:0016874
PubMed:  11342140|15582399|14529378

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK12467 super family cl36129
peptide synthase; Provisional
 44-2119 0e+00

peptide synthase; Provisional


The actual alignment was detected with superfamily member PRK12467:

Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1578.63  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   44 PGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQTAPAAPVA 123
Cdd:PRK12467   47 FERIPLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFV-QDEEGFRQVIDASLSLT 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  124 LPVIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA-- 194
Cdd:PRK12467  126 IPLDDLAneqgrarESQIEAYINEEVARPFDLANGPLLRVRLLRLADDEHVLVVTLHHIISDGWSMRVLVEELVQLYSay 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  195 --GELPAGVAAP-AFRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAV 270
Cdd:PRK12467  206 sqGREPSLPALPiQYADYAIWQRSWLEAGERERQLAYWQEQLGGeHTVLELPTDRPRPAVPSYRGARLRVDLPQALSAGL 285

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  271 RRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGS 350
Cdd:PRK12467  286 KALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVETERLIGFFVNTQVLKAEVDPQASFLELLQQVKRTA 365

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  351 RNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVALTFGEAAG----TEQPVDVA--LAKCDLDLSVLDDGDR 424
Cdd:PRK12467  366 LGAQAHQDLPFEQLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQlpglTVEELSWArhTAQFDLALDTYESAQG 445

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAASLRERFQQWCRRT 504
Cdd:PRK12467  446 LWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDCVHQLIEAQARQH 525

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PRK12467  526 PERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYML 605

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLG-PDDITGLVVLEETD-TGGYPATEP-PAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:PRK12467  606 DDSGVRLLLTQSHLLAQLPvPAGLRSLCLDEPADlLCGYSGHNPeVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYV 685

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvpeL 741
Cdd:PRK12467  686 CVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLKIVPSHLQAL---L 762

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGPALAGIR----VLVGGEALPAELA---RRLGDaGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYV 812
Cdd:PRK12467  763 QASRVALPRpqraLVCGGEALQVDLLarvRALGP-GARLINHYGPTETTVGVSTYELSDEERDFGNvpIGQPLANLGLYI 841

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:PRK12467  842 LDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGADGGRLYRTGDLARYRADGVIEYLGRMDHQVKIRGF 921

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLInAGPVRRAAAVVREDRPGDLRLVAYVIPDGP-------VAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:PRK12467  922 RIELGEIEARLL-AQPGVREAVVLAQPGDAGLQLVAYLVPAAVadgaehqATRDELKAQLRQVLPDYMVPAHLLLLDSLP 1000

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  966 TTPNGKLDRAALPAPDYGT-RSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFS 1044
Cdd:PRK12467 1001 LTPNGKLDRKALPKPDASAvQATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGGHSLLATQVISRVRQRLGIQVP 1080

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1045 LRRLLAAPTVAAVAAELAAETSTGDDAvhLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRR 1124
Cdd:PRK12467 1081 LRTLFEHQTLAGFAQAVAAQQQGAQPA--LPDVDRDQPLPLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALER 1158

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1125 TLNRVVDRHEMLRTRIHPgPDGMPVQVADPAGGGA---ALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDE 1201
Cdd:PRK12467 1159 SFDALVARHESLRTTFVQ-EDGRTRQVIHPVGSLTleePLLLAADKDEAQLKVYVEAEARQPFDLEQGPLLRVGLLRLAA 1237

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1202 REHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVA 1281
Cdd:PRK12467 1238 DEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQYADYAVWQRQWMDAGERARQLAYWKAQLGGEQP 1317

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1282 T-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLD 1360
Cdd:PRK12467 1318 VlELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANRNRAETE 1397

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1361 NLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQ-EPAGRSA 1435
Cdd:PRK12467 1398 GLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQLVealqPERSLSHSPLFQVMFNHQRDdHQAQAQL 1477

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1436 GDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTV 1515
Cdd:PRK12467 1478 PGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIERLAGHWLNLLQGLVADPERRLGELDLLDEAERR 1557

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1516 RT-SRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLD 1594
Cdd:PRK12467 1558 QIlEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLI--ALG---VGPEVLVGIAVE 1632

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1595 RSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfgDVPVVALDRVADRLTAMPDQ 1674
Cdd:PRK12467 1633 RSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHLQARLPLPD---GLRSLVLDQEDDWLEGYSDS 1709

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1675 RPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTL 1754
Cdd:PRK12467 1710 NPAVNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVI 1789

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MPADATLAdlAEELSGPLAYDYIRL---TPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHY 1831
Cdd:PRK12467 1790 APPGAHRD--PEQLIQLIERQQVTTlhfVPSMLQQLLQMDEQVEHPLSLRRVVCGGEALEVEALRPWLERLPDTGLFNLY 1867

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETVIGRVVHPVREAGELAVDSpLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:PRK12467 1868 GPTETAVDVTHWTCRRKDLEGRDS-VPIGQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVAD 1946

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 PAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIP-- 1985
Cdd:PRK12467 1947 PFGTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDgangKQLVAYVVPtd 2026

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1986 ----AEDHPPVTV-SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRPelEDRYQAPRTPGEQLLAE 2060
Cdd:PRK12467 2027 pglvDDDEAQVALrAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKALPAPDASEL--QQAYVAPQSELEQRLAA 2104

                        2090      2100      2110      2120      2130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 2061 LWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAGVEISPKDVFEQPTVRGQAMRA 2119
Cdd:PRK12467 2105 IWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLAAVA 2163
Abhydrolase super family cl21494
alpha/beta hydrolases; A functionally diverse superfamily containing proteases, lipases, ...
 2157-2311 4.40e-29

alpha/beta hydrolases; A functionally diverse superfamily containing proteases, lipases, peroxidases, esterases, epoxide hydrolases and dehalogenases. The catalytic apparatus typically involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine, and often the mechanism involves a nucleophilic attack on a carbonyl carbon atom.


The actual alignment was detected with superfamily member pfam00975:

Pssm-ID: 473884 [Multi-domain]  Cd Length: 223  Bit Score: 117.49  E-value: 4.40e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2157 RPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGTIAF 2236
Cdd:pfam00975    1 RPLFCFPPAGGSASSFRSLARRLPPPAEVLAVQYPGRGRGEPPLNSIEALADEYAEALRQIQPEGPYALFGHSMGGMLAF 80

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  2237 EMARQLDRLGEPVQ-LILLEPTLPEVARTIDLHRPARDSYLrgaDLAERILRLPAGDPEGDRLRAELTRLLEDAGY 2311
Cdd:pfam00975   81 EVARRLERQGEAVRsLFLSDASAPHTVRYEASRAPDDDEVV---AEFTDEGGTPEELLEDEELLSMLLPALRADYR 153
 
Name Accession Description Interval E-value
PRK12467 PRK12467
peptide synthase; Provisional
 44-2119 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1578.63  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   44 PGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQTAPAAPVA 123
Cdd:PRK12467   47 FERIPLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFV-QDEEGFRQVIDASLSLT 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  124 LPVIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA-- 194
Cdd:PRK12467  126 IPLDDLAneqgrarESQIEAYINEEVARPFDLANGPLLRVRLLRLADDEHVLVVTLHHIISDGWSMRVLVEELVQLYSay 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  195 --GELPAGVAAP-AFRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAV 270
Cdd:PRK12467  206 sqGREPSLPALPiQYADYAIWQRSWLEAGERERQLAYWQEQLGGeHTVLELPTDRPRPAVPSYRGARLRVDLPQALSAGL 285

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  271 RRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGS 350
Cdd:PRK12467  286 KALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVETERLIGFFVNTQVLKAEVDPQASFLELLQQVKRTA 365

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  351 RNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVALTFGEAAG----TEQPVDVA--LAKCDLDLSVLDDGDR 424
Cdd:PRK12467  366 LGAQAHQDLPFEQLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQlpglTVEELSWArhTAQFDLALDTYESAQG 445

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAASLRERFQQWCRRT 504
Cdd:PRK12467  446 LWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDCVHQLIEAQARQH 525

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PRK12467  526 PERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYML 605

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLG-PDDITGLVVLEETD-TGGYPATEP-PAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:PRK12467  606 DDSGVRLLLTQSHLLAQLPvPAGLRSLCLDEPADlLCGYSGHNPeVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYV 685

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvpeL 741
Cdd:PRK12467  686 CVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLKIVPSHLQAL---L 762

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGPALAGIR----VLVGGEALPAELA---RRLGDaGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYV 812
Cdd:PRK12467  763 QASRVALPRpqraLVCGGEALQVDLLarvRALGP-GARLINHYGPTETTVGVSTYELSDEERDFGNvpIGQPLANLGLYI 841

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:PRK12467  842 LDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGADGGRLYRTGDLARYRADGVIEYLGRMDHQVKIRGF 921

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLInAGPVRRAAAVVREDRPGDLRLVAYVIPDGP-------VAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:PRK12467  922 RIELGEIEARLL-AQPGVREAVVLAQPGDAGLQLVAYLVPAAVadgaehqATRDELKAQLRQVLPDYMVPAHLLLLDSLP 1000

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  966 TTPNGKLDRAALPAPDYGT-RSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFS 1044
Cdd:PRK12467 1001 LTPNGKLDRKALPKPDASAvQATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGGHSLLATQVISRVRQRLGIQVP 1080

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1045 LRRLLAAPTVAAVAAELAAETSTGDDAvhLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRR 1124
Cdd:PRK12467 1081 LRTLFEHQTLAGFAQAVAAQQQGAQPA--LPDVDRDQPLPLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALER 1158

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1125 TLNRVVDRHEMLRTRIHPgPDGMPVQVADPAGGGA---ALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDE 1201
Cdd:PRK12467 1159 SFDALVARHESLRTTFVQ-EDGRTRQVIHPVGSLTleePLLLAADKDEAQLKVYVEAEARQPFDLEQGPLLRVGLLRLAA 1237

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1202 REHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVA 1281
Cdd:PRK12467 1238 DEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQYADYAVWQRQWMDAGERARQLAYWKAQLGGEQP 1317

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1282 T-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLD 1360
Cdd:PRK12467 1318 VlELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANRNRAETE 1397

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1361 NLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQ-EPAGRSA 1435
Cdd:PRK12467 1398 GLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQLVealqPERSLSHSPLFQVMFNHQRDdHQAQAQL 1477

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1436 GDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTV 1515
Cdd:PRK12467 1478 PGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIERLAGHWLNLLQGLVADPERRLGELDLLDEAERR 1557

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1516 RT-SRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLD 1594
Cdd:PRK12467 1558 QIlEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLI--ALG---VGPEVLVGIAVE 1632

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1595 RSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfgDVPVVALDRVADRLTAMPDQ 1674
Cdd:PRK12467 1633 RSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHLQARLPLPD---GLRSLVLDQEDDWLEGYSDS 1709

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1675 RPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTL 1754
Cdd:PRK12467 1710 NPAVNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVI 1789

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MPADATLAdlAEELSGPLAYDYIRL---TPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHY 1831
Cdd:PRK12467 1790 APPGAHRD--PEQLIQLIERQQVTTlhfVPSMLQQLLQMDEQVEHPLSLRRVVCGGEALEVEALRPWLERLPDTGLFNLY 1867

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETVIGRVVHPVREAGELAVDSpLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:PRK12467 1868 GPTETAVDVTHWTCRRKDLEGRDS-VPIGQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVAD 1946

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 PAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIP-- 1985
Cdd:PRK12467 1947 PFGTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDgangKQLVAYVVPtd 2026

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1986 ----AEDHPPVTV-SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRPelEDRYQAPRTPGEQLLAE 2060
Cdd:PRK12467 2027 pglvDDDEAQVALrAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKALPAPDASEL--QQAYVAPQSELEQRLAA 2104

                        2090      2100      2110      2120      2130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 2061 LWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAGVEISPKDVFEQPTVRGQAMRA 2119
Cdd:PRK12467 2105 IWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLAAVA 2163
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 39-1336 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 963.55  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   39 LPVQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLgPDGEPVQQTAP 118
Cdd:COG1020    10 PPAAAAAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRT-RAGRPVQVIQP 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  119 AAPVALPVIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLAR 191
Cdd:COG1020    89 VVAAPLPVVVLLvdlealaEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLLLLLLLLALHHIISDGLSDGLLLAELLR 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  192 AY-----AGELPAGVAAPAFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAA 265
Cdd:COG1020   169 LYlaayaGAPLPLPPLPIQYADYALWQREWLQGEELARQLAYWRQQLAGLPPlLELPTDRPRPAVQSYRGARVSFRLPAE 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  266 TEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSR 345
Cdd:COG1020   249 LTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPRPELEGLVGFFVNTLPLRVDLSGDPSFAELLAR 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  346 TVAGSRNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAV-ALTFGEAAGTEQPVDVALAKCDLDLSVLDDGDR 424
Cdd:COG1020   329 VRETLLAAYAHQDLPFERLVEELQPERDLSRNPLFQVMFVLQNAPAdELELPGLTLEPLELDSGTAKFDLTLTVVETGDG 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPA-ASLRERFQQWCRR 503
Cdd:COG1020   409 LRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPAdATLHELFEAQAAR 488

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  504 TPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYL 583
Cdd:COG1020   489 TPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYM 568

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDAATADRLgPDDITGLVVLEETDTGGYPATEPPA-VPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLA 662
Cdd:COG1020   569 LEDAGARLVLTQSALAARL-PELGVPVLALDALALAAEPATNPPVpVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLA 647

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  663 AMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELS 742
Cdd:COG1020   648 WMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLRALLDAAP 727

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  743 GPALAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYVLDANLH 818
Cdd:COG1020   728 EALPSLRLVLVGGEALPPELVRRWRARlpGARLVNLYGPTETTVDSTYYEVTPPDADGGSvpIGRPIANTRVYVLDAHLQ 807

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  819 PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGE 898
Cdd:COG1020   808 PVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFGFPGARLYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIELGE 887

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  899 IETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDG--PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:COG1020   888 IEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAgaAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLA 967

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  977 LPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFSLRRLLAAPTVAA 1056
Cdd:COG1020   968 LPAPAAAAAAAAAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLLLLLLFLAAAAAA 1047

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1057 VAAELAAETSTGDDAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEML 1136
Cdd:COG1020  1048 AAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLALLLLLALLALLALLLLLLLLLLLLALLLLLALLLALLAALRA 1127

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1137 RTRIHPGPDGMPVQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVD 1216
Cdd:COG1020  1128 RRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAELLAAAALLLLLALLLLALLLLLLLLLLLLLLLLLLLLLLLL 1207

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1217 AWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATEVAGDLPRPATPGG 1296
Cdd:COG1020  1208 LLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALAALAALAAALLALALALLALALLLLALALLLPALARAR 1287

                        1290      1300      1310      1320
                  ....*....|....*....|....*....|....*....|
gi 653678460 1297 GGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLL 1336
Cdd:COG1020  1288 AARTARALALLLLLALLLLLALALALLLLLLLLLALLLLA 1327
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 505-977 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 602.21  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAatadrlgpdditglvvleetdtggypateppavpaGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd05930    81 EDSGAKLVLTDP-----------------------------------DDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLWM 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGP 744
Cdd:cd05930   126 QEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGIR-VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYVLDANLHP 819
Cdd:cd05930   206 ALPSLRlVLVGGEALPPDLVRRWRELlpGARLVNLYGPTEATVDATYYRVPPDDEEDGRvpIGRPIPNTRVYVLDENLRP 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  820 APIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEI 899
Cdd:cd05930   286 VPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFG-PGERMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEI 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  900 ETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05930   365 EAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDeeELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 518-915 1.16e-147

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 465.59  E-value: 1.16e-147
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   518 SYRELDERANRLAR-LLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:TIGR01733    1 TYRELDERANRLARhLRAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   597 ATADRLGPDDITGLVV--LEETDTGGYPATEPPAVPAGHS--AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTA 672
Cdd:TIGR01733   81 ALASRLAGLVLPVILLdpLELAALDDAPAPPPPDAPSGPDdlAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   673 EDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHR-VTLAQATPTLWQALVPELsGPALAGIR- 750
Cdd:TIGR01733  161 DDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDAALLAALIAEHpVTVLNLTPSLLALLAAAL-PPALASLRl 239

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   751 VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS---IGVPIDNTQVYVLDANLHPAPIGVL 825
Cdd:TIGR01733  240 VILGGEALTPALVDRWRARgpGARLINLYGPTETTVWSTATLVDPDDAPRESpvpIGRPLANTRLYVLDDDLRPVPVGVV 319

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   826 GELHIAGEGLARGYWNRPGLTAEKFLPDPF-GPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLi 904
Cdd:TIGR01733  320 GELYIGGPGVARGYLNRPELTAERFVPDPFaGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAAL- 398

                          410
                   ....*....|.
gi 653678460   905 NAGPVRRAAAV 915
Cdd:TIGR01733  399 LRHPGVREAVV 409
AMP-binding pfam00501
AMP-binding enzyme;
497-890 7.16e-107

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 348.92  E-value: 7.16e-107
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   497 FQQWCRRTPGAVAVIEGD-TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:pfam00501    1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   576 PVERIAYLLSDARPVLVVTDAAT------ADRLGPDDITGLVVLEETDTGGY------------PATEPPAVPAGHSAYV 637
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALkleellEALGKLEVVKLVLVLDRDPVLKEeplpeeakpadvPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   638 IYTSGSTGRPKGVVITRAALDNFLAAMA----ERFPLTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLADADTARNP 712
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGlSLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   713 AALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTS 788
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLeaGAPKRALLSSLRlVLSGGAPLPPELARRFRELfGGALVNGYGLTETTGVVTT 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   789 GPTSEDSIRR-GSIGVPIDNTQVYVLDAN-LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtRMYRTG 866
Cdd:pfam00501  321 PLPLDEDLRSlGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED-------GWYRTG 393

                          410       420
                   ....*....|....*....|....
gi 653678460   867 DLVRWSAAGDLEYLGRTDHQVKLR 890
Cdd:pfam00501  394 DLGRRDEDGYLEIVGRKKDQIKLG 417
Thioesterase pfam00975
Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of ...
 2157-2311 4.40e-29

Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 395776 [Multi-domain]  Cd Length: 223  Bit Score: 117.49  E-value: 4.40e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2157 RPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGTIAF 2236
Cdd:pfam00975    1 RPLFCFPPAGGSASSFRSLARRLPPPAEVLAVQYPGRGRGEPPLNSIEALADEYAEALRQIQPEGPYALFGHSMGGMLAF 80

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  2237 EMARQLDRLGEPVQ-LILLEPTLPEVARTIDLHRPARDSYLrgaDLAERILRLPAGDPEGDRLRAELTRLLEDAGY 2311
Cdd:pfam00975   81 EVARRLERQGEAVRsLFLSDASAPHTVRYEASRAPDDDEVV---AEFTDEGGTPEELLEDEELLSMLLPALRADYR 153
GrsT COG3208
Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and ...
 2159-2309 1.78e-14

Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442441 [Multi-domain]  Cd Length: 237  Bit Score: 75.27  E-value: 1.78e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2159 VFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGR--EAPAETIEDMAAGYLADLRLvQPEGPYVVVGWSLGGTIAF 2236
Cdd:COG3208     9 LFCFPYAGGSASAYRPWAAALPPDIEVLAVQLPGRGDRlgEPPLTSLEELADDLAEELAP-LLDRPFALFGHSMGALLAF 87

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 2237 EMARQLDRLG--EPVQLIL---LEPTLPEVARTIDLhrpardsyLRGADLAERILRLpAGDPEGDRLRAELTRLLEDA 2309
Cdd:COG3208    88 ELARRLERRGrpLPAHLFVsgrRAPHLPRRRRPLHD--------LSDAELLAELRRL-GGTPEEVLADPELLELFLPI 156
PRK14875 PRK14875
acetoin dehydrogenase E2 subunit dihydrolipoyllysine-residue acetyltransferase; Provisional
 2132-2240 2.60e-04

acetoin dehydrogenase E2 subunit dihydrolipoyllysine-residue acetyltransferase; Provisional


Pssm-ID: 184875 [Multi-domain]  Cd Length: 371  Bit Score: 45.71  E-value: 2.60e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2132 LDTVFGAGHTGTT--RTVVTLR-GEGDGRPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQpL---GMDGREAPAETIED 2205
Cdd:PRK14875  104 EEDAGPAPRKARIggRTVRYLRlGEGDGTPVVLIHGFGGDLNNWLFNHAALAAGRPVIALD-LpghGASSKAVGAGSLDE 182

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 653678460 2206 MAAGYLADLRLVQPEgPYVVVGWSLGGTIAFEMAR 2240
Cdd:PRK14875  183 LAAAVLAFLDALGIE-RAHLVGHSMGGAVALRLAA 216
bioH TIGR01738
pimelyl-[acyl-carrier protein] methyl ester esterase; This CoA-binding enzyme is required for ...
 2154-2239 9.88e-04

pimelyl-[acyl-carrier protein] methyl ester esterase; This CoA-binding enzyme is required for the production of pimeloyl-coenzyme A, the substrate of the BioF protein early in the biosynthesis of biotin. Its exact function is unknown, but is proposed in ref 2. This enzyme belongs to the alpha/beta hydrolase fold family (pfam00561). Members of this family are restricted to the Proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin]


Pssm-ID: 273783 [Multi-domain]  Cd Length: 245  Bit Score: 43.27  E-value: 9.88e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2154 GDGRP-VFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGM-DGREAPAETIEDMAAGYLAdlrlvQPEGPYVVVGWSLG 2231
Cdd:TIGR01738    1 GQGNVhLVLIHGWGMNAEVFRCLDEELSAHFTLHLVDLPGHgRSRGFGPLSLADMAEAIAA-----QAPDPAIWLGWSLG 75


                   ....*...
gi 653678460  2232 GTIAFEMA 2239
Cdd:TIGR01738   76 GLVALHIA 83
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 2055-2116 1.19e-03

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 39.93  E-value: 1.19e-03
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460   2055 EQLLAELWSTVLG---TDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVRGQA 2116
Cdd:smart00823   14 LDLVREQVAAVLGhaaAEAIDPDRPFRDLGLDSLMAVELRNRLEAAtGLRLPATLVFDHPTPAALA 79
 
Name Accession Description Interval E-value
PRK12467 PRK12467
peptide synthase; Provisional
 44-2119 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 1578.63  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   44 PGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQTAPAAPVA 123
Cdd:PRK12467   47 FERIPLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFV-QDEEGFRQVIDASLSLT 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  124 LPVIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA-- 194
Cdd:PRK12467  126 IPLDDLAneqgrarESQIEAYINEEVARPFDLANGPLLRVRLLRLADDEHVLVVTLHHIISDGWSMRVLVEELVQLYSay 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  195 --GELPAGVAAP-AFRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAV 270
Cdd:PRK12467  206 sqGREPSLPALPiQYADYAIWQRSWLEAGERERQLAYWQEQLGGeHTVLELPTDRPRPAVPSYRGARLRVDLPQALSAGL 285

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  271 RRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGS 350
Cdd:PRK12467  286 KALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVETERLIGFFVNTQVLKAEVDPQASFLELLQQVKRTA 365

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  351 RNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVALTFGEAAG----TEQPVDVA--LAKCDLDLSVLDDGDR 424
Cdd:PRK12467  366 LGAQAHQDLPFEQLVEALQPERSLSHSPLFQVMFNHQNTATGGRDREGAQlpglTVEELSWArhTAQFDLALDTYESAQG 445

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAASLRERFQQWCRRT 504
Cdd:PRK12467  446 LWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDCVHQLIEAQARQH 525

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PRK12467  526 PERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYML 605

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLG-PDDITGLVVLEETD-TGGYPATEP-PAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:PRK12467  606 DDSGVRLLLTQSHLLAQLPvPAGLRSLCLDEPADlLCGYSGHNPeVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYV 685

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvpeL 741
Cdd:PRK12467  686 CVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLKIVPSHLQAL---L 762

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGPALAGIR----VLVGGEALPAELA---RRLGDaGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYV 812
Cdd:PRK12467  763 QASRVALPRpqraLVCGGEALQVDLLarvRALGP-GARLINHYGPTETTVGVSTYELSDEERDFGNvpIGQPLANLGLYI 841

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:PRK12467  842 LDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGADGGRLYRTGDLARYRADGVIEYLGRMDHQVKIRGF 921

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLInAGPVRRAAAVVREDRPGDLRLVAYVIPDGP-------VAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:PRK12467  922 RIELGEIEARLL-AQPGVREAVVLAQPGDAGLQLVAYLVPAAVadgaehqATRDELKAQLRQVLPDYMVPAHLLLLDSLP 1000

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  966 TTPNGKLDRAALPAPDYGT-RSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFS 1044
Cdd:PRK12467 1001 LTPNGKLDRKALPKPDASAvQATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGGHSLLATQVISRVRQRLGIQVP 1080

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1045 LRRLLAAPTVAAVAAELAAETSTGDDAvhLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRR 1124
Cdd:PRK12467 1081 LRTLFEHQTLAGFAQAVAAQQQGAQPA--LPDVDRDQPLPLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALER 1158

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1125 TLNRVVDRHEMLRTRIHPgPDGMPVQVADPAGGGA---ALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDE 1201
Cdd:PRK12467 1159 SFDALVARHESLRTTFVQ-EDGRTRQVIHPVGSLTleePLLLAADKDEAQLKVYVEAEARQPFDLEQGPLLRVGLLRLAA 1237

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1202 REHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVA 1281
Cdd:PRK12467 1238 DEHVLVLTLHHIVSDGWSMQVLVDELVALYAAYSQGQSLQLPALPIQYADYAVWQRQWMDAGERARQLAYWKAQLGGEQP 1317

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1282 T-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLD 1360
Cdd:PRK12467 1318 VlELPTDRPRPAVQSHRGARLAFELPPALAEGLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANRNRAETE 1397

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1361 NLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQ-EPAGRSA 1435
Cdd:PRK12467 1398 GLIGFFVNTQVLRAEVDGQASFQQLLQQVKQAALEAQAHQDLPFEQLVealqPERSLSHSPLFQVMFNHQRDdHQAQAQL 1477

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1436 GDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTV 1515
Cdd:PRK12467 1478 PGLSVESLSWESQTAQFDLTLDTYESSEGLQASLTYATDLFEASTIERLAGHWLNLLQGLVADPERRLGELDLLDEAERR 1557

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1516 RT-SRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLD 1594
Cdd:PRK12467 1558 QIlEGWNATHTGYPLARLVHQLIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLI--ALG---VGPEVLVGIAVE 1632

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1595 RSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfgDVPVVALDRVADRLTAMPDQ 1674
Cdd:PRK12467 1633 RSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQSHLQARLPLPD---GLRSLVLDQEDDWLEGYSDS 1709

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1675 RPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTL 1754
Cdd:PRK12467 1710 NPAVNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVI 1789

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MPADATLAdlAEELSGPLAYDYIRL---TPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHY 1831
Cdd:PRK12467 1790 APPGAHRD--PEQLIQLIERQQVTTlhfVPSMLQQLLQMDEQVEHPLSLRRVVCGGEALEVEALRPWLERLPDTGLFNLY 1867

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETVIGRVVHPVREAGELAVDSpLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:PRK12467 1868 GPTETAVDVTHWTCRRKDLEGRDS-VPIGQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVAD 1946

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 PAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIP-- 1985
Cdd:PRK12467 1947 PFGTVGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDgangKQLVAYVVPtd 2026

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1986 ----AEDHPPVTV-SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRPelEDRYQAPRTPGEQLLAE 2060
Cdd:PRK12467 2027 pglvDDDEAQVALrAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKALPAPDASEL--QQAYVAPQSELEQRLAA 2104

                        2090      2100      2110      2120      2130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 2061 LWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAGVEISPKDVFEQPTVRGQAMRA 2119
Cdd:PRK12467 2105 IWQDVLGLEQVGLHDNFFELGGDSIISIQVVSRARQAGIRFTPKDLFQHQTVQSLAAVA 2163
PRK05691 PRK05691
peptide synthase; Validated
 32-2129 0e+00

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 1323.64  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   32 GPAHGGGLPVQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFrLGPDGE 111
Cdd:PRK05691  661 GAAQAAIARLPRGQALPQSLAQNRLWLLWQLDPQSAAYNIPGGLHLRGELDEAALRASFQRLVERHESLRTRF-YERDGV 739

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  112 PVQQTAPAAPVALPVIDVTeaDLPQALRTA---------AEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSC 182
Cdd:PRK05691  740 ALQRIDAQGEFALQRIDLS--DLPEAEREAraaqireeeARQPFDLEKGPLLRVTLVRLDDEEHQLLVTLHHIVADGWSL 817

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  183 GLLLADLARAYAGE---LPAGVAAPAFR--DVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAH 256
Cdd:PRK05691  818 NILLDEFSRLYAAAcqgQTAELAPLPLGyaDYGAWQRQWLAQGEAARQLAYWKAQLGDeQPVLELATDHPRSARQAHSAA 897

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  257 SVRRELPAATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGR 336
Cdd:PRK05691  898 RYSLRVDASLSEALRGLAQAHQATLFMVLLAAFQALLHRYSGQGDIRIGVPNANRPRLETQGLVGFFINTQVLRAQLDGR 977

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  337 PSFDEVLSRTVAGSRNDFAHPQIPFDRLVRELRRdriAGSQLLVQAMAGTES---TAVALTFGEAAgTEQPVDVALAKCD 413
Cdd:PRK05691  978 LPFTALLAQVRQATLGAQAHQDLPFEQLVEALPQ---AREQGLFQVMFNHQQrdlSALRRLPGLLA-EELPWHSREAKFD 1053

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  414 LDL-SVLDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTvLCRWNDTARPLPAAS 492
Cdd:PRK05691 1054 LQLhSEEDRNGRLTLSFDYAAELFDAATIERLAEHFLALLEQVCEDPQRALGDVQLLDAAERAQ-LAQWGQAPCAPAQAW 1132

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  493 LRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:PRK05691 1133 LPELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLD 1212

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFPVERIAYLLSDARPVLVVTDAATADRLGPDDITGLVVLEETDTGGYPATEPP-AVPAGHSAYVIYTSGSTGRPKGVV 651
Cdd:PRK05691 1213 PDYPAERLAYMLADSGVELLLTQSHLLERLPQAEGVSAIALDSLHLDSWPSQAPGlHLHGDNLAYVIYTSGSTGQPKGVG 1292

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATP 731
Cdd:PRK05691 1293 NTHAALAERLQWMQATYALDDSDVLMQKAPISFDVSVWECFWPLITGCRLVLAGPGEHRDPQRIAELVQQYGVTTLHFVP 1372

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQALVPElsgPALAGI----RVLVGGEALPAELARRLGDAGAEVT--NMYGPTETTIWSTSGPTSEDSIRRGSIGVPI 805
Cdd:PRK05691 1373 PLLQLFIDE---PLAAACtslrRLFSGGEALPAELRNRVLQRLPQVQlhNRYGPTETAINVTHWQCQAEDGERSPIGRPL 1449

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  806 DNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDH 885
Cdd:PRK05691 1450 GNVLCRVLDAELNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQ 1529

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  886 QVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDlRLVAYVIPDG--PVAPDALRTELSRTLPDYMIPAVIVPVPD 963
Cdd:PRK05691 1530 QVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAGA-QLVGYYTGEAgqEAEAERLKAALAAELPEYMVPAQLIRLDQ 1608

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  964 FPTTPNGKLDRAALPAPDYGTRSTARpPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGF 1043
Cdd:PRK05691 1609 MPLGPSGKLDRRALPEPVWQQREHVE-PRTELQQQIAAIWREVLGLPRVGLRDDFFALGGHSLLATQIVSRTRQACDVEL 1687

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1044 SLRRLLAAPTVAAVAAELAAETSTGDDAVH--LAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGA 1121
Cdd:PRK05691 1688 PLRALFEASELGAFAEQVARIQAAGERNSQgaIARVDRSQPVPLSYSQQRMWFLWQMEPDSPAYNVGGMARLSGVLDVDR 1767

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1122 LRRTLNRVVDRHEMLRTRIhPGPDGMPVQ-VADPAGGGAALTERDAAPGTDLAQLLLAEAALG----FTLATEHPLRAVL 1196
Cdd:PRK05691 1768 FEAALQALILRHETLRTTF-PSVDGVPVQqVAEDSGLRMDWQDFSALPADARQQRLQQLADSEahqpFDLERGPLLRACL 1846

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1197 WRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTEL 1276
Cdd:PRK05691 1847 VKAAEREHYFVLTLHHIVTEGWAMDIFARELGALYEAFLDDRESPLEPLPVQYLDYSVWQRQWLESGERQRQLDYWKAQL 1926

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1277 -TGAVATEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRG 1355
Cdd:PRK05691 1927 gNEHPLLELPADRPRPPVQSHRGELYRFDLSPELAARVRAFNAQRGLTLFMTMTATLAALLYRYSGQRDLRIGAPVANRI 2006

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1356 NPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIM-----YSF- 1425
Cdd:PRK05691 2007 RPESEGLIGAFLNTQVLRCQLDGQMSVSELLEQVRQTVIEGQSHQDLPFDHLVealqPPRSAAYNPLFQVMcnvqrWEFq 2086

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1426 SSQEPAGrsagdVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTR 1505
Cdd:PRK05691 2087 QSRQLAG-----MTVEYLVNDARATKFDLNLEVTDLDGRLGCCLTYSRDLFDEPRIARMAEHWQNLLEALLGDPQQRLAE 2161

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1506 LAITSESD-TVRTSRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgalgAPPVG 1584
Cdd:PRK05691 2162 LPLLAAAEqQQLLDSLAGEAGEARLDQTLHGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALR-----ERGVG 2236

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1585 PETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLtrtGQGGCLpgaEVFGDVPV-VA--- 1660
Cdd:PRK05691 2237 PQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLL---SDRALF---EALGELPAgVArwc 2310

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1661 LDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMS 1740
Cdd:PRK05691 2311 LEDDAAALAAYSDAPLPFLSLPQHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSINFDAASE 2390

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1741 ALFEPLVSGRGVTLMPADATLADLAEELSGPLAYDYIRLTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLE 1820
Cdd:PRK05691 2391 RLLVPLLCGARVVLRAQGQWGAEEICQLIREQQVSILGFTPSYGSQLAQWLAGQGEQLPVRMCITGGEALTGEHLQRIRQ 2470

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1821 LRPDAEVINHYGPTETVIGRVVHPVREAGELAVDSpLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGR 1900
Cdd:PRK05691 2471 AFAPQLFFNAYGPTETVVMPLACLAPEQLEEGAAS-VPIGRVVGARVAYILDADLALVPQGATGELYVGGAGLAQGYHDR 2549

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1901 PALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD---- 1976
Cdd:PRK05691 2550 PGLTAERFVADPFAADGGRLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLALDtpsg 2629

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1977 QQLVGWFIPA-----EDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTsgrPEL-EDRYQAP 2050
Cdd:PRK05691 2630 KQLAGYLVSAvagqdDEAQAALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRALPAPD---PELnRQAYQAP 2706

                        2090      2100      2110      2120      2130      2140      2150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 2051 RTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAGVEISPKDVFEQPTVrgQAMRAIARSRDDAYA 2129
Cdd:PRK05691 2707 RSELEQQLAQIWREVLNVERVGLGDNFFELGGDSILSIQVVSRARQLGIHFSPRDLFQHQTV--QTLAAVATHSEAAQA 2783
PRK12316 PRK12316
peptide synthase; Provisional
 38-2116 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 1296.85  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   38 GLPVqPPGPV----PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGpLDVDALRAALDAVTARHEALRTVF-RLGPDGEP 112
Cdd:PRK12316 1545 ALPL-PAGEIadiyPLSPMQQGMLFHSLYEQEAGDYINQLRVDVQG-LDPDRFRAAWQATVDRHEILRSGFlWQDGLEQP 1622

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  113 VQQTAPAapVALPVIDV---TEADLPQALRTAA----EQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLL 185
Cdd:PRK12316 1623 LQVIHKQ--VELPFAELdwrGREDLGQALDALAqaerQKGFDLTRAPLLRLVLVRTGEGRHHLIYTNHHILMDGWSNAQL 1700

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  186 LADLARAYAGELPAGvAAPAFRDVAAW-QHGRLAAGElddqlRYWRQRLAGLPDLEIPGDRQRPADRDWRAHSVRRELPA 264
Cdd:PRK12316 1701 LGEVLQRYAGQPVAA-PGGRYRDYIAWlQRQDAAASE-----AFWKEQLAALEEPTRLAQAARTEDGQVGYGDHQQLLDP 1774

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  265 ATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGR--TDLESVVGLFAGMVPV----RLDLTGRPS 338
Cdd:PRK12316 1775 AQTRALAEFARAQKVTLNTLVQAAWLLLLQRYTGQETVAFGATVAGRPAelPGIEQQIGLFINTLPViaapRPDQSVADW 1854

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  339 FDEVLSRTVAgsRNDFAHPQIpfdrlvRELRRDRIAGSQLLVQAMAGTESTAVA----------LTFGEAAGTEQ---PV 405
Cdd:PRK12316 1855 LQEVQALNLA--LREHEHTPL------YDIQRWAGQGGEALFDSLLVFENYPVAealkqgapagLVFGRVSNHEQtnyPL 1926

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  406 DVALAKcdldlsvlddGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTA 485
Cdd:PRK12316 1927 TLAVTL----------GETLSLQYSYDRGHFDAAAIERLDRHLLHLLEQMAEDAQAALGELALLDAGERQRILADWDRTP 1996

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  486 RPLPAA-SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKA 564
Cdd:PRK12316 1997 EAYPRGpGVHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKA 2076

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  565 GAAYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRLGPDdiTGLVVLEETDTG---GYPATEP-PAVPAGHSAYVIYT 640
Cdd:PRK12316 2077 GGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERLPLP--AGVARLPLDRDAewaDYPDTAPaVQLAGENLAYVIYT 2154

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  641 SGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADaDTARNPAALIDLAG 720
Cdd:PRK12316 2155 SGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHPLLNGARVLIRD-DELWDPEQLYDEME 2233

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQATPTLWQALVPEL--SGPALAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWST---SGPTSE 793
Cdd:PRK12316 2234 RHGVTILDFPPVYLQQLAEHAerDGRPPAVRVYCFGGEAVPAASLRLAWEAlrPVYLFNGYGPTEAVVTPLlwkCRPQDP 2313

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  794 DSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSA 873
Cdd:PRK12316 2314 CGAAYVPIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFSASGERLYRTGDLARYRA 2393

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  874 AGDLEYLGRTDHQVKLRGFRIELGEIETTLInAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVA--PDALRTELSRTLPD 951
Cdd:PRK12316 2394 DGVVEYLGRIDHQVKIRGFRIELGEIEARLQ-AHPAVREAVVVAQDGASGKQLVAYVVPDDAAEdlLAELRAWLAARLPA 2472

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTA-RPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFR 1030
Cdd:PRK12316 2473 YMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQLRQAyVAPQEGLEQRLAAIWQAVLKVEQVGLDDHFFELGGHSLLATQ 2552

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1031 LLARIRDEFGAGFSLRRLLAAPTVAAVAAELAAETSTgdDAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLA 1110
Cdd:PRK12316 2553 VVSRVRQDLGLEVPLRILFERPTLAAFAASLESGQTS--RAPVLQKVTRVQPLPLSHAQQRQWFLWQLEPESAAYHLPSA 2630

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1111 LRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEH 1190
Cdd:PRK12316 2631 LHLRGVLDQAALEQAFDALVLRHETLRTRFVEVGEQTRQVILPNMSLRIVLEDCAGVADAAIRQRVAEEIQRPFDLARGP 2710

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1191 PLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELD 1270
Cdd:PRK12316 2711 LLRVRLLALDGQEHVLVITQHHIVSDGWSMQVMVDELVQAYAGARRGEQPTLPPLPLQYADYAAWQRAWMDSGEGARQLD 2790

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1271 FWRTELTGA-VATEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGS 1349
Cdd:PRK12316 2791 YWRERLGGEqPVLELPLDRPRPALQSHRGARLDVALDVALSRELLALARREGVTLFMLLLASFQVLLHRYSGQSDIRVGV 2870

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1350 TVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLVA----DRGAGNGPLFRIMYSF 1425
Cdd:PRK12316 2871 PIANRNRAETERLIGFFVNTQVLRAQVDAQLAFRDLLGQVKEQALGAQAHQDLPFEQLVEalqpERSLSHSPLFQVMYNH 2950

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1426 SSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTR 1505
Cdd:PRK12316 2951 QSGERAAAQLPGLHIESFAWDGAATQFDLALDTWESAEGLGASLTYATDLFDARTVERLARHWQNLLRGMVENPQRSVDE 3030

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1506 LAITSESDTVR-TSRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgalgAPPVG 1584
Cdd:PRK12316 3031 LAMLDAEERGQlLEAWNATAAEYPLERGVHRLFEEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLI-----ERGVG 3105

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1585 PETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGqggcLPGAEVFGdVPVVALDRV 1664
Cdd:PRK12316 3106 PDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQSH----LRLPLAQG-VQVLDLDRG 3180

                        1690      1700      1710      1720      1730      1740      1750      1760
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1665 ADRLTampDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFE 1744
Cdd:PRK12316 3181 DENYA---EANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTTFSFDVFVEELFW 3257

                        1770      1780      1790      1800      1810      1820      1830      1840
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1745 PLVSGRGVTLM-PADATLADLAEELSGPLAYDYIRLTPSHLRHLVGHWTGQELpPAARGWVVGGETLDPALVKQLLELRP 1823
Cdd:PRK12316 3258 PLMSGARVVLAgPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRC-TSLKRIVCGGEALPADLQQQVFAGLP 3336

                        1850      1860      1870      1880      1890      1900      1910      1920
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1824 daeVINHYGPTETVIGRVVHPVREAGElavdSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPAL 1903
Cdd:PRK12316 3337 ---LYNLYGPTEATITVTHWQCVEEGK----DAVPIGRPIANRACYILDGSLEPVPVGALGELYLGGEGLARGYHNRPGL 3409

                        1930      1940      1950      1960      1970      1980      1990      2000
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1904 TAEKFLPDPAgEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQLVGW 1982
Cdd:PRK12316 3410 TAERFVPDPF-VPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLaVDGRQLVAY 3488

                        2010      2020      2030      2040      2050      2060      2070      2080
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1983 FIPaEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGrpELEDRYQAPRTPGEQLLAELW 2062
Cdd:PRK12316 3489 VVP-EDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAA--LLQQDYVAPVNELERRLAAIW 3565

                        2090      2100      2110      2120      2130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 653678460 2063 STVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAGVEISPKDVFEQPTVRGQA 2116
Cdd:PRK12316 3566 ADVLKLEQVGLTDNFFELGGDSIISLQVVSRARQAGIRFTPKDLFQHQTIQGLA 3619
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 39-1336 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 963.55  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   39 LPVQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLgPDGEPVQQTAP 118
Cdd:COG1020    10 PPAAAAAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRT-RAGRPVQVIQP 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  119 AAPVALPVIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLAR 191
Cdd:COG1020    89 VVAAPLPVVVLLvdlealaEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLLLLLLLLALHHIISDGLSDGLLLAELLR 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  192 AY-----AGELPAGVAAPAFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAA 265
Cdd:COG1020   169 LYlaayaGAPLPLPPLPIQYADYALWQREWLQGEELARQLAYWRQQLAGLPPlLELPTDRPRPAVQSYRGARVSFRLPAE 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  266 TEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSR 345
Cdd:COG1020   249 LTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPRPELEGLVGFFVNTLPLRVDLSGDPSFAELLAR 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  346 TVAGSRNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAV-ALTFGEAAGTEQPVDVALAKCDLDLSVLDDGDR 424
Cdd:COG1020   329 VRETLLAAYAHQDLPFERLVEELQPERDLSRNPLFQVMFVLQNAPAdELELPGLTLEPLELDSGTAKFDLTLTVVETGDG 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPA-ASLRERFQQWCRR 503
Cdd:COG1020   409 LRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQLLAEWNATAAPYPAdATLHELFEAQAAR 488

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  504 TPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYL 583
Cdd:COG1020   489 TPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYM 568

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDAATADRLgPDDITGLVVLEETDTGGYPATEPPA-VPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLA 662
Cdd:COG1020   569 LEDAGARLVLTQSALAARL-PELGVPVLALDALALAAEPATNPPVpVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLA 647

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  663 AMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELS 742
Cdd:COG1020   648 WMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLTPSLLRALLDAAP 727

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  743 GPALAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYVLDANLH 818
Cdd:COG1020   728 EALPSLRLVLVGGEALPPELVRRWRARlpGARLVNLYGPTETTVDSTYYEVTPPDADGGSvpIGRPIANTRVYVLDAHLQ 807

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  819 PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGE 898
Cdd:COG1020   808 PVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFGFPGARLYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIELGE 887

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  899 IETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDG--PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:COG1020   888 IEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAgaAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLA 967

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  977 LPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFSLRRLLAAPTVAA 1056
Cdd:COG1020   968 LPAPAAAAAAAAAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAARLLLLLLLLLLLFLAAAAAA 1047

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1057 VAAELAAETSTGDDAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEML 1136
Cdd:COG1020  1048 AAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLALLLLLALLALLALLLLLLLLLLLLALLLLLALLLALLAALRA 1127

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1137 RTRIHPGPDGMPVQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVD 1216
Cdd:COG1020  1128 RRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAELLAAAALLLLLALLLLALLLLLLLLLLLLLLLLLLLLLLLL 1207

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1217 AWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATEVAGDLPRPATPGG 1296
Cdd:COG1020  1208 LLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALAALAALAAALLALALALLALALLLLALALLLPALARAR 1287

                        1290      1300      1310      1320
                  ....*....|....*....|....*....|....*....|
gi 653678460 1297 GGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLL 1336
Cdd:COG1020  1288 AARTARALALLLLLALLLLLALALALLLLLLLLLALLLLA 1327
PRK12467 PRK12467
peptide synthase; Provisional
 31-1347 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 869.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   31 QGPAHGGGLPVQPPG-PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLgPD 109
Cdd:PRK12467 1100 QQQGAQPALPDVDRDqPLPLSYAQERQWFLWQLEPGSAAYHIPQALRLKGPLDIEALERSFDALVARHESLRTTFVQ-ED 1178

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  110 GEPVQQTAPAAPVA-----LPVIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGL 184
Cdd:PRK12467 1179 GRTRQVIHPVGSLTleeplLLAADKDEAQLKVYVEAEARQPFDLEQGPLLRVGLLRLAADEHVLVLTLHHIVSDGWSMQV 1258

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  185 LLADLARAYA----GELPAGVAAP-AFRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSV 258
Cdd:PRK12467 1259 LVDELVALYAaysqGQSLQLPALPiQYADYAVWQRQWMDAGERARQLAYWKAQLGGeQPVLELPTDRPRPAVQSHRGARL 1338

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  259 RRELPAATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPS 338
Cdd:PRK12467 1339 AFELPPALAEGLRALARREGVTLFMLLLASFQTLLHRYSGQDDIRVGVPIANRNRAETEGLIGFFVNTQVLRAEVDGQAS 1418

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  339 FDEVLSRTVAGSRNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAG--TESTAVALTFGEAAGTEQPVDVALAKCDLDL 416
Cdd:PRK12467 1419 FQQLLQQVKQAALEAQAHQDLPFEQLVEALQPERSLSHSPLFQVMFNhqRDDHQAQAQLPGLSVESLSWESQTAQFDLTL 1498

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  417 SVLDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAA-SLRE 495
Cdd:PRK12467 1499 DTYESSEGLQASLTYATDLFEASTIERLAGHWLNLLQGLVADPERRLGELDLLDEAERRQILEGWNATHTGYPLArLVHQ 1578

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  496 RFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:PRK12467 1579 LIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEY 1658

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  576 PVERIAYLLSDARPVLVVTDAATADRLG-PDDITGLVVLEETD-TGGYPATEPPAVPAGHS-AYVIYTSGSTGRPKGVVI 652
Cdd:PRK12467 1659 PRERLAYMIEDSGIELLLTQSHLQARLPlPDGLRSLVLDQEDDwLEGYSDSNPAVNLAPQNlAYVIYTSGSTGRPKGAGN 1738

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  653 TRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPT 732
Cdd:PRK12467 1739 RHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPS 1818

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  733 LWQALV--PELSGPALAGIRVLVGGEALPAELAR----RLGDAGaeVTNMYGPTETTIWSTSGP-TSEDSIRRGS--IGV 803
Cdd:PRK12467 1819 MLQQLLqmDEQVEHPLSLRRVVCGGEALEVEALRpwleRLPDTG--LFNLYGPTETAVDVTHWTcRRKDLEGRDSvpIGQ 1896

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  804 PIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRT 883
Cdd:PRK12467 1897 PIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFGTVGSRLYRTGDLARYRADGVIEYLGRI 1976

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  884 DHQVKLRGFRIELGEIETTLINAGPVRRaAAVVREDRPGDLRLVAYVIPDGPVAPDA----------LRTELSRTLPDYM 953
Cdd:PRK12467 1977 DHQVKIRGFRIELGEIEARLREQGGVRE-AVVIAQDGANGKQLVAYVVPTDPGLVDDdeaqvalraiLKNHLKASLPEYM 2055

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  954 IPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTA-RPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLL 1032
Cdd:PRK12467 2056 VPAHLVFLARMPLTPNGKLDRKALPAPDASELQQAyVAPQSELEQRLAAIWQDVLGLEQVGLHDNFFELGGDSIISIQVV 2135

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1033 ARIRDEfGAGFSLRRLLAAPTVAAVAAELaaetSTGDDAVHLAPVPRDEPLPLSPMQESIWlaDQVSAGDSVYNVPLALR 1112
Cdd:PRK12467 2136 SRARQA-GIRFTPKDLFQHQTVQSLAAVA----QEGDGTVSIDQGPVTGDLPLLPIQQMFF--ADDIPERHHWNQSVLLE 2208

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1113 LIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEHPL 1192
Cdd:PRK12467 2209 PREALDAELLEAALQALLVHHDALRLGFVQEDGGWSAMHRAPEQERRPLLWQVVVADKEELEALCEQAQRSLDLEEGPLL 2288

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1193 RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFW 1272
Cdd:PRK12467 2289 RAVLATLPDGSQRLLLVIHHLVVDGVSWRILLEDLQTAYRQLQGGQPVKLPAKTSAFKAWAERLQTYAASAALADELGYW 2368

                        1290      1300      1310      1320      1330      1340      1350
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1273 RTELTGAvATEVAGDLPRPATPGGGGGAVEFALAPRQI-QGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVI 1347
Cdd:PRK12467 2369 QAQLQGA-STELPCDHPQGGLQRRHAASVTTHLDSEWTrRLLQEAPAAYRTQVNDLLLTALARVIARWTGQASTLI 2443
PRK12316 PRK12316
peptide synthase; Provisional
 41-1347 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 862.34  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   41 VQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDgEPVQQTAPAA 120
Cdd:PRK12316   44 VSSAERDRLSYAQQRMWFLWQLEPQSGAYNLPSAVRLNGPLDRQALERAFASLVQRHETLRTVFPRGAD-DSLAQVPLDR 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  121 PVALPVIDVT---EADLPQALRTAAE----QPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY 193
Cdd:PRK12316  123 PLEVEFEDCSglpEAEQEARLRDEAQreslQPFDLCEGPLLRVRLLRLGEEEHVLLLTLHHIVSDGWSMNVLIEEFSRFY 202

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  194 AG-ELPAGVAAPA----FRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATE 267
Cdd:PRK12316  203 SAyATGAEPGLPAlpiqYADYALWQRSWLEAGEQERQLEYWRAQLGEeHPVLELPTDHPRPAVPSYRGSRYEFSIDPALA 282

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  268 AAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLS--- 344
Cdd:PRK12316  283 EALRGTARRQGLTLFMLLLGAFNVLLHRYSGQTDIRVGVPIANRNRAEVEGLIGFFVNTQVLRSVFDGRTRVATLLAgvk 362

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  345 RTVAGSRndfAHPQIPFDRLVRELRRDRIAGSQLLVQAM---------AGTESTAVALTFGEAagteqPVDVALAKCDLD 415
Cdd:PRK12316  363 DTVLGAQ---AHQDLPFERLVEALKVERSLSHSPLFQVMynhqplvadIEALDTVAGLEFGQL-----EWKSRTTQFDLT 434

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  416 LSVLDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLP-AASLR 494
Cdd:PRK12316  435 LDTYEKGGRLHAALTYATDLFEARTVERMARHWQNLLRGMVENPQARVDELPMLDAEERGQLVEGWNATAAEYPlQRGVH 514

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:PRK12316  515 RLFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPE 594

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAATADRLGPDDITGLVVLEETDT--GGYPaTEPP--AVPAGHSAYVIYTSGSTGRPKGV 650
Cdd:PRK12316  595 YPAERLAYMLEDSGVQLLLSQSHLGRKLPLAAGVQVLDLDRPAAwlEGYS-EENPgtELNPENLAYVIYTSGSTGKPKGA 673

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  651 VITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQAT 730
Cdd:PRK12316  674 GNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGARLVVAAPGDHRDPAKLVELINREGVDTLHFV 753

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  731 PTLWQALVPELSGPALAGIRVLV-GGEALPAELARRLGD--AGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDN 807
Cdd:PRK12316  754 PSMLQAFLQDEDVASCTSLRRIVcSGEALPADAQEQVFAklPQAGLYNLYGPTEAAIDVTHWTCVEEGGDSVPIGRPIAN 833

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  808 TQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQV 887
Cdd:PRK12316  834 LACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFV-AGERMYRTGDLARYRADGVIEYAGRIDHQV 912

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  888 KLRGFRIELGEIETTLINAGPVRRAAAVVREDRpgdlRLVAYVIPD--GPVAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:PRK12316  913 KLRGLRIELGEIEARLLEHPWVREAAVLAVDGK----QLVGYVVLEseGGDWREALKAHLAASLPEYMVPAQWLALERLP 988

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  966 TTPNGKLDRAALPAPDYGTRSTAR-PPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEfGAGFS 1044
Cdd:PRK12316  989 LTPNGKLDRKALPAPEASVAQQGYvAPRNALERTLAAIWQDVLGVERVGLDDNFFELGGDSIVSIQVVSRARQA-GIQLS 1067

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1045 LRRLLAAPTVAAVAAELAAETSTGDDAvhlapVPRDEPLPLSPMQEsiWLADQVSAGDSVYNVPLALRLIGPLDRGALRR 1124
Cdd:PRK12316 1068 PRDLFQHQTIRSLALVAKAGQATAADQ-----GPASGEVALAPVQR--WFFEQAIPQRQHWNQSLLLQARQPLDPDRLGR 1140

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1125 TLNRVVDRHEMLRTRIHpGPDGMPVQVADPAGGGAALTERDAApgtDLAQLLLAEAALGFTLATEHP--LRAVLWRLDER 1202
Cdd:PRK12316 1141 ALERLVAHHDALRLRFR-EEDGGWQQAYAAPQAGEVLWQRQAA---SEEELLALCEEAQRSLDLEQGplLRALLVDMADG 1216

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1203 EHVLLLTAHHVAVDAWSMDLIQRDLTELYaadtghREPELDEPALAQAdYAVW-QRQSAQRGRYAAELDFWRTELTGAvA 1281
Cdd:PRK12316 1217 SQRLLLVIHHLVVDGVSWRILLEDLQRAY------ADLDADLPARTSS-YQAWaRRLHEHAGARAEELDYWQAQLEDA-P 1288

                        1290      1300      1310      1320      1330      1340
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1282 TEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRC-GTTVSTVVLSALQTLLYRITGGADVVI 1347
Cdd:PRK12316 1289 HELPCENPDGALENRHERKLELRLDAERTRQLLQEAPAAyRTQVNDLLLTALARVTCRWSGQASVLV 1355
PRK12316 PRK12316
peptide synthase; Provisional
 40-1354 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 805.33  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   40 PVQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlgPDGEPVQQTAPA 119
Cdd:PRK12316 2596 KVTRVQPLPLSHAQQRQWFLWQLEPESAAYHLPSALHLRGVLDQAALEQAFDALVLRHETLRTRFV--EVGEQTRQVILP 2673

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  120 A----PVALPVIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG 195
Cdd:PRK12316 2674 NmslrIVLEDCAGVADAAIRQRVAEEIQRPFDLARGPLLRVRLLALDGQEHVLVITQHHIVSDGWSMQVMVDELVQAYAG 2753

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  196 -----ELPAGVAAPAFRDVAAWQHGRLAAGELDDQLRYWRQRLAGL-PDLEIPGDRQRPADRDWRAHSVRRELPAATEAA 269
Cdd:PRK12316 2754 arrgeQPTLPPLPLQYADYAAWQRAWMDSGEGARQLDYWRERLGGEqPVLELPLDRPRPALQSHRGARLDVALDVALSRE 2833

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  270 VRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAG 349
Cdd:PRK12316 2834 LLALARREGVTLFMLLLASFQVLLHRYSGQSDIRVGVPIANRNRAETERLIGFFVNTQVLRAQVDAQLAFRDLLGQVKEQ 2913

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  350 SRNDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVA-LTFGEAAGTEQPVDVALAKCDLDLSVLDDGDRLAVT 428
Cdd:PRK12316 2914 ALGAQAHQDLPFEQLVEALQPERSLSHSPLFQVMYNHQSGERAaAQLPGLHIESFAWDGAATQFDLALDTWESAEGLGAS 2993

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  429 LVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAASLRER-FQQWCRRTPGA 507
Cdd:PRK12316 2994 LTYATDLFDARTVERLARHWQNLLRGMVENPQRSVDELAMLDAEERGQLLEAWNATAAEYPLERGVHRlFEEQVERTPDA 3073

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  508 VAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDA 587
Cdd:PRK12316 3074 VALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDS 3153

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  588 RPVLVVTDAATadRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAER 667
Cdd:PRK12316 3154 GAQLLLSQSHL--RLPLAQGVQVLDLDRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQA 3231

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  668 FPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPALA 747
Cdd:PRK12316 3232 YGLGVGDRVLQFTTFSFDVFVEELFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRCT 3311

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  748 GIR-VLVGGEALPAELARRLgDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLG 826
Cdd:PRK12316 3312 SLKrIVCGGEALPADLQQQV-FAGLPLYNLYGPTEATITVTHWQCVEEGKDAVPIGRPIANRACYILDGSLEPVPVGALG 3390

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  827 ELHIAGEGLARGYWNRPGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINA 906
Cdd:PRK12316 3391 ELYLGGEGLARGYHNRPGLTAERFVPDPF-VPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEH 3469

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  907 GPVRRAAAVVREDRpgdlRLVAYVIPDGPVA--PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGT 984
Cdd:PRK12316 3470 PWVREAVVLAVDGR----QLVAYVVPEDEAGdlREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAAL 3545

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  985 RSTAR-PPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRdEFGAGFSLRRLLAAPTVAAVAAELAa 1063
Cdd:PRK12316 3546 LQQDYvAPVNELERRLAAIWADVLKLEQVGLTDNFFELGGDSIISLQVVSRAR-QAGIRFTPKDLFQHQTIQGLARVAR- 3623

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1064 etstGDDAVHLAPVPRDEPLPLSPMQESIWlaDQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPG 1143
Cdd:PRK12316 3624 ----VGGGVAVDQGPVSGETLLLPIQQQFF--EEPVPERHHWNQSLLLKPREALDAAALEAALQALVEHHDALRLRFVED 3697

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1144 PDGMPVQVADPAGGGAALTERDAApGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLI 1223
Cdd:PRK12316 3698 AGGWTAEHLPVELGGALLWRAELD-DAEELERLGEEAQRSLDLADGPLLRALLATLADGSQRLLLVIHHLVVDGVSWRIL 3776

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1224 QRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGaVATEVAGDLPRPATPGGGGGAVEF 1303
Cdd:PRK12316 3777 LEDLQQAYQQLLQGEAPRLPAKTSSFKAWAERLQEHARGEALKAELAYWQEQLQG-VSSELPCDHPQGALQNRHAASVQT 3855

                        1290      1300      1310      1320      1330
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 1304 ALAprqiqgiRELARRC--------GTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR 1354
Cdd:PRK12316 3856 RLD-------RELTRRLlqqapaayRTQVNDLLLTALARVVCRWTGEASALVQLEGHGR 3907
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
 1069-2393 0e+00

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 796.37  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1069 DDAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMP 1148
Cdd:COG1020     4 AAAAALPPAAAAAPLPLSAAQQRLWLLLLLLLGSAAYNLALALLLLGLLLVAALLLLAALLARRRRALRTRLRTRAGRPV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1149 VQVADPAGGGA----ALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQ 1224
Cdd:COG1020    84 QVIQPVVAAPLpvvvLLVDLEALAEAAAEAAAAAEALAPFDLLRGPLLRLLLLLLLLLLLLLLLALHHIISDGLSDGLLL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1225 RDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVAT-EVAGDLPRPATPGGGGGAVEF 1303
Cdd:COG1020   164 AELLRLYLAAYAGAPLPLPPLPIQYADYALWQREWLQGEELARQLAYWRQQLAGLPPLlELPTDRPRPAVQSYRGARVSF 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1304 ALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFH 1383
Cdd:COG1020   244 RLPAELTAALRALARRHGVTLFMVLLAAFALLLARYSGQDDVVVGTPVAGRPRPELEGLVGFFVNTLPLRVDLSGDPSFA 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1384 ELLRRTHARTADALAHQELPFEHLVAD----RGAGNGPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVV 1459
Cdd:COG1020   324 ELLARVRETLLAAYAHQDLPFERLVEElqpeRDLSRNPLFQVMFVLQNAPADELELPGLTLEPLELDSGTAKFDLTLTVV 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1460 DGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVR-TSRWGRSEQHVGDDRTVHQLVE 1538
Cdd:COG1020   404 ETGDGLRLTLEYNTDLFDAATIERMAGHLVTLLEALAADPDQPLGDLPLLTAAERQQlLAEWNATAAPYPADATLHELFE 483

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1539 AQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDP 1618
Cdd:COG1020   484 AQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLR--ALG---VGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDP 558

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1619 GYPMARLTRIADTVTPALVLTRTGQGGCLPGAevfgDVPVVALDRVAdrLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQP 1698
Cdd:COG1020   559 AYPAERLAYMLEDAGARLVLTQSALAARLPEL----GVPVLALDALA--LAAEPATNPPVPVTPDDLAYVIYTSGSTGRP 632

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1699 KGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATL--ADLAEELSGPlAYDY 1776
Cdd:COG1020   633 KGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRdpAALAELLARH-RVTV 711

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1777 IRLTPSHLRHLVGHwtGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGelAVDSP 1856
Cdd:COG1020   712 LNLTPSLLRALLDA--APEALPSLRLVLVGGEALPPELVRRWRARLPGARLVNLYGPTETTVDSTYYEVTPPD--ADGGS 787

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1857 LPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLD 1936
Cdd:COG1020   788 VPIGRPIANTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFGFPGARLYRTGDLARWLPDGNLE 867

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1937 FVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPN 2012
Cdd:COG1020   868 FLGRADDQVKIRGFRIELGEIEAALLQHPGVREAVVVAREdapgDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPA 947

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2013 QWVALDAFPLTPHGKVNHRALPGPTSGRPelEDRYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLSG 2092
Cdd:COG1020   948 AVVLLLPLPLTGNGKLDRLALPAPAAAAA--AAAAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALAR 1025

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2093 ACRRAGVEISPKDVFEQPTVRGQAMRAIARSRDDAYAPVLDTVFGAGHTGTT-RTVVTLRGEGDGRPVFCVHPSGGGVAW 2171
Cdd:COG1020  1026 AARLLLLLLLLLLLFLAAAAAAAAAAAAAAAAAAAAPLAAAAAPLPLPPLLLsLLALLLALLLLLALLALLALLLLLLLL 1105

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2172 YLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGTIAFEMARQLDRLGEPVQL 2251
Cdd:COG1020  1106 LLLLALLLLLALLLALLAALRARRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAELLAAAALLLLLALLLLALL 1185

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2252 ILLEPTLPEVARTIDLHRPARDSYLRGADLAERILRLPAGDPEGDRLRAELTRLLEDAGYSAGEISLGDALPMRACGQML 2331
Cdd:COG1020  1186 LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAALLALAALAALAAALLALALA 1265

                        1290      1300      1310      1320      1330      1340
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 2332 AAFAEYRPLPLPDESKVRTQFVVSAECTDATPERPSGTSHTAYGSYAAGWQKLLGRAAAPRT 2393
Cdd:COG1020  1266 LLALALLLLALALLLPALARARAARTARALALLLLLALLLLLALALALLLLLLLLLALLLLA 1327
PRK12467 PRK12467
peptide synthase; Provisional
 1076-2123 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 784.74  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1076 PVPRD----EPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQV 1151
Cdd:PRK12467   39 PIPQVrsafERIPLSYAQERQWFLWQLDPDSAAYNIPTALRLRGELDVSALRRAFDALVARHESLRTRFVQDEEGFRQVI 118

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1152 ADPAGGGAALTERDAAPGTDL----AQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDL 1227
Cdd:PRK12467  119 DASLSLTIPLDDLANEQGRAResqiEAYINEEVARPFDLANGPLLRVRLLRLADDEHVLVVTLHHIISDGWSMRVLVEEL 198

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1228 TELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTEL-TGAVATEVAGDLPRPATPGGGGGAVEFALA 1306
Cdd:PRK12467  199 VQLYSAYSQGREPSLPALPIQYADYAIWQRSWLEAGERERQLAYWQEQLgGEHTVLELPTDRPRPAVPSYRGARLRVDLP 278

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1307 PRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELL 1386
Cdd:PRK12467  279 QALSAGLKALAQREGVTLFMVLLASFQTLLHRYSGQSDIRIGVPNANRNRVETERLIGFFVNTQVLKAEVDPQASFLELL 358

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1387 RRTHARTADALAHQELPFEHLVA----DRGAGNGPLFRIMYSFSSQEPAGRSA-----GDVDLEPVPVPVTTCKFDLVLT 1457
Cdd:PRK12467  359 QQVKRTALGAQAHQDLPFEQLVEalqpERSLSHSPLFQVMFNHQNTATGGRDRegaqlPGLTVEELSWARHTAQFDLALD 438

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1458 VVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRTSRWGRSEQHVGDDRTVHQLV 1537
Cdd:PRK12467  439 TYESAQGLWAAFTYATDLFEATTIERLATHWRNLLEAIVAEPRRRLGELPLLDAEERARELVRWNAPATEYAPDCVHQLI 518

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1538 EAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgalgAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD 1617
Cdd:PRK12467  519 EAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLI-----AAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLD 593

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPMARLTRIADTVTPALVLTRTGQGGCLPgaeVFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQ 1697
Cdd:PRK12467  594 PEYPQDRLAYMLDDSGVRLLLTQSHLLAQLP---VPAGLRSLCLDEPADLLCGYSGHNPEVALDPDNLAYVIYTSGSTGQ 670

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1698 PKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLAdlAEELSGPLA---Y 1774
Cdd:PRK12467  671 PKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARD--AEAFAALMAdqgV 748

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1775 DYIRLTPSHLRHLVGHwTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGelAVD 1854
Cdd:PRK12467  749 TVLKIVPSHLQALLQA-SRVALPRPQRALVCGGEALQVDLLARVRALGPGARLINHYGPTETTVGVSTYELSDEE--RDF 825

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1855 SPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGL 1934
Cdd:PRK12467  826 GNVPIGQPLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGADGGRLYRTGDLARYRADGV 905

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1935 LDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPAED----HPPVTVSALRRFCAEQLP 2006
Cdd:PRK12467  906 IEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPgdagLQLVAYLVPAAVadgaEHQATRDELKAQLRQVLP 985

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2007 EFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRPelEDRYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSIS 2086
Cdd:PRK12467  986 DYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASAV--QATFVAPQTELEKRLAAIWADVLKVERVGLTDNFFELGGHSLL 1063

                        1050      1060      1070
                  ....*....|....*....|....*....|....*...
gi 653678460 2087 VIQ-LSGACRRAGVEISPKDVFEQPTVRGQAMRAIARS 2123
Cdd:PRK12467 1064 ATQvISRVRQRLGIQVPLRTLFEHQTLAGFAQAVAAQQ 1101
PRK12316 PRK12316
peptide synthase; Provisional
 1075-2119 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 774.90  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1075 APVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPG--------PDG 1146
Cdd:PRK12316   42 AGVSSAERDRLSYAQQRMWFLWQLEPQSGAYNLPSAVRLNGPLDRQALERAFASLVQRHETLRTVFPRGaddslaqvPLD 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1147 MPVQVADPAGGGAALTERDAApgtdLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRD 1226
Cdd:PRK12316  122 RPLEVEFEDCSGLPEAEQEAR----LRDEAQRESLQPFDLCEGPLLRVRLLRLGEEEHVLLLTLHHIVSDGWSMNVLIEE 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1227 LTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVAT-EVAGDLPRPATPGGGGGAVEFAL 1305
Cdd:PRK12316  198 FSRFYSAYATGAEPGLPALPIQYADYALWQRSWLEAGEQERQLEYWRAQLGEEHPVlELPTDHPRPAVPSYRGSRYEFSI 277

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1306 APRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHEL 1385
Cdd:PRK12316  278 DPALAEALRGTARRQGLTLFMLLLGAFNVLLHRYSGQTDIRVGVPIANRNRAEVEGLIGFFVNTQVLRSVFDGRTRVATL 357

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1386 LRRTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQ--EPAGRS-AGDVDLEPVPVPVTTCKFDLVLTV 1458
Cdd:PRK12316  358 LAGVKDTVLGAQAHQDLPFERLVealkVERSLSHSPLFQVMYNHQPLvaDIEALDtVAGLEFGQLEWKSRTTQFDLTLDT 437

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1459 VDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRTSR-WGRSEQHVGDDRTVHQLV 1537
Cdd:PRK12316  438 YEKGGRLHAALTYATDLFEARTVERMARHWQNLLRGMVENPQARVDELPMLDAEERGQLVEgWNATAAEYPLQRGVHRLF 517

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1538 EAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD 1617
Cdd:PRK12316  518 EEQVERTPEAPALAFGEETLDYAELNRRANRLAHALI--ERG---VGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLD 592

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPMARLTRIADTVTPALVLTRTGQGGCLPgaeVFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQ 1697
Cdd:PRK12316  593 PEYPAERLAYMLEDSGVQLLLSQSHLGRKLP---LAAGVQVLDLDRPAAWLEGYSEENPGTELNPENLAYVIYTSGSTGK 669

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1698 PKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSG-RGVTLMPADATLADLAEELSGPLAYDY 1776
Cdd:PRK12316  670 PKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGaRLVVAAPGDHRDPAKLVELINREGVDT 749

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1777 IRLTPSHLRHLVgHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELAVdsp 1856
Cdd:PRK12316  750 LHFVPSMLQAFL-QDEDVASCTSLRRIVCSGEALPADAQEQVFAKLPQAGLYNLYGPTEAAIDVTHWTCVEEGGDSV--- 825

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1857 lPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEpGARMYRTGDLVRWRGDGLLD 1936
Cdd:PRK12316  826 -PIGRPIANLACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFVA-GERMYRTGDLARYRADGVIE 903

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1937 FVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-QQLVGWFIPaEDHPPVTVSALRRFCAEQLPEFMVPNQWV 2015
Cdd:PRK12316  904 YAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVDgKQLVGYVVL-ESEGGDWREALKAHLAASLPEYMVPAQWL 982

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2016 ALDAFPLTPHGKVNHRALPGPTSGRPELEdrYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACR 2095
Cdd:PRK12316  983 ALERLPLTPNGKLDRKALPAPEASVAQQG--YVAPRNALERTLAAIWQDVLGVERVGLDDNFFELGGDSIVSIQVVSRAR 1060

                        1050      1060
                  ....*....|....*....|....
gi 653678460 2096 RAGVEISPKDVFEQPTVRGQAMRA 2119
Cdd:PRK12316 1061 QAGIQLSPRDLFQHQTIRSLALVA 1084
PRK05691 PRK05691
peptide synthase; Validated
 492-2108 0e+00

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 752.39  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  492 SLRERFQQWCRRTPGAVAVI------EGDTTLSYRELDERANRLARLLMERGAGAETfvAVLL-PRSADLLVTLLAVIKA 564
Cdd:PRK05691   10 TLVQALQRRAAQTPDRLALRfladdpGEGVVLSYRDLDLRARTIAAALQARASFGDR--AVLLfPSGPDYVAAFFGCLYA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  565 GAAYLPIDPDFPV-----ERIAYLLSDARPVLVVTDAATAD------RLGPDDITGLVVLEETDTGGYPATEPPAVPAGH 633
Cdd:PRK05691   88 GVIAVPAYPPESArrhhqERLLSIIADAEPRLLLTVADLRDsllqmeELAAANAPELLCVDTLDPALAEAWQEPALQPDD 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  634 SAYVIYTSGSTGRPKGVVITRAAL--DNFLAAMAERFPLTAEDRVLATTTVSFDIaGL--ELYLPLRSGAGVVL-ADADT 708
Cdd:PRK05691  168 IAFLQYTSGSTALPKGVQVSHGNLvaNEQLIRHGFGIDLNPDDVIVSWLPLYHDM-GLigGLLQPIFSGVPCVLmSPAYF 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  709 ARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPALAGI-----RVLVGG-----EALPAELARRLGDAGAEVTNM-- 776
Cdd:PRK05691  247 LERPLRWLEAISEYGGTISGGPDFAYRLCSERVSESALERLdlsrwRVAYSGsepirQDSLERFAEKFAACGFDPDSFfa 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  777 -YGPTETTIWSTSGPT--------------SEDSIRRG------SIGVPIDNTQVYVLDAN-LHPAPIGVLGELHIAGEG 834
Cdd:PRK05691  327 sYGLAEATLFVSGGRRgqgipaleldaealARNRAEPGtgsvlmSCGRSQPGHAVLIVDPQsLEVLGDNRVGEIWASGPS 406

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  835 LARGYWNRPGLTAEKFLPDPfgppGTRMYRTGDLvRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR--- 911
Cdd:PRK05691  407 IAHGYWRNPEASAKTFVEHD----GRTWLRTGDL-GFLRDGELFVTGRLKDMLIVRGHNLYPQDIEKTVEREVEVVRkgr 481

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  912 --AAAVVREDRPG------DLRLVAYVIPdgpvaPDALRTELSRTLPD--YMIPAVIVPVPD--FPTTPNGKLDRAA--- 976
Cdd:PRK05691  482 vaAFAVNHQGEEGigiaaeISRSVQKILP-----PQALIKSIRQAVAEacQEAPSVVLLLNPgaLPKTSSGKLQRSAcrl 556

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  977 ------------LPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFS 1044
Cdd:PRK05691  557 rladgsldsyalFPALQAVEAAQTAASGDELQARIAAIWCEQLKVEQVAADDHFFLLGGNSIAATQVVARLRDELGIDLN 636

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1045 LRRLLAAPTVAAVAAELAAETSTGDDAVH-LAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALR 1123
Cdd:PRK05691  637 LRQLFEAPTLAAFSAAVARQLAGGGAAQAaIARLPRGQALPQSLAQNRLWLLWQLDPQSAAYNIPGGLHLRGELDEAALR 716

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1124 RTLNRVVDRHEMLRTRIHPgPDGMPVQVADPAG---------GGAALTERDAAPGTDLAQLLLAEaalgFTLATEHPLRA 1194
Cdd:PRK05691  717 ASFQRLVERHESLRTRFYE-RDGVALQRIDAQGefalqridlSDLPEAEREARAAQIREEEARQP----FDLEKGPLLRV 791

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1195 VLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRT 1274
Cdd:PRK05691  792 TLVRLDDEEHQLLVTLHHIVADGWSLNILLDEFSRLYAAACQGQTAELAPLPLGYADYGAWQRQWLAQGEAARQLAYWKA 871

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1275 ELTGAVAT-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSG 1353
Cdd:PRK05691  872 QLGDEQPVlELATDHPRSARQAHSAARYSLRVDASLSEALRGLAQAHQATLFMVLLAAFQALLHRYSGQGDIRIGVPNAN 951

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1354 RGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLV-ADRGAGNGPLFRIMYSFSSQEP-A 1431
Cdd:PRK05691  952 RPRLETQGLVGFFINTQVLRAQLDGRLPFTALLAQVRQATLGAQAHQDLPFEQLVeALPQAREQGLFQVMFNHQQRDLsA 1031

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1432 GRSAGDVDLEPVPVPVTTCKFDLVL-TVVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITS 1510
Cdd:PRK05691 1032 LRRLPGLLAEELPWHSREAKFDLQLhSEEDRNGRLTLSFDYAAELFDAATIERLAEHFLALLEQVCEDPQRALGDVQLLD 1111

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1511 ESDTVRTSRWGRSEQhVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHAL-HTGalgappVGPETPV 1589
Cdd:PRK05691 1112 AAERAQLAQWGQAPC-APAQAWLPELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLrDKG------VGPDVCV 1184

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1590 LLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfgDVPVVALDRVadRLT 1669
Cdd:PRK05691 1185 AIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAERLAYMLADSGVELLLTQSHLLERLPQAE---GVSAIALDSL--HLD 1259

                        1290      1300      1310      1320      1330      1340      1350      1360
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1670 AMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSG 1749
Cdd:PRK05691 1260 SWPSQAPGLHLHGDNLAYVIYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSDVLMQKAPISFDVSVWECFWPLITG 1339

                        1370      1380      1390      1400      1410      1420      1430      1440
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1750 -RGVTLMPAD----ATLADLAEELSgplaYDYIRLTPSHLRHLVghwtgqELPPAA-----RGWVVGGETLDPALVKQLL 1819
Cdd:PRK05691 1340 cRLVLAGPGEhrdpQRIAELVQQYG----VTTLHFVPPLLQLFI------DEPLAAactslRRLFSGGEALPAELRNRVL 1409

                        1450      1460      1470      1480      1490      1500      1510      1520
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1820 ELRPDAEVINHYGPTETVIgRVVH--PVREAGELAvdsplPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGY 1897
Cdd:PRK05691 1410 QRLPQVQLHNRYGPTETAI-NVTHwqCQAEDGERS-----PIGRPLGNVLCRVLDAELNLLPPGVAGELCIGGAGLARGY 1483

                        1530      1540      1550      1560      1570      1580      1590      1600
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1898 LGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD- 1976
Cdd:PRK05691 1484 LGRPALTAERFVPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREg 1563

                        1610      1620      1630      1640      1650      1660      1670      1680
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1977 ---QQLVGWFiPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRPEledrYQAPRTP 2053
Cdd:PRK05691 1564 aagAQLVGYY-TGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRALPEPVWQQRE----HVEPRTE 1638

                        1690      1700      1710      1720      1730
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 2054 GEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFE 2108
Cdd:PRK05691 1639 LQQQIAAIWREVLGLPRVGLRDDFFALGGHSLLATQIVSRTRQAcDVELPLRALFE 1694
PRK12316 PRK12316
peptide synthase; Provisional
 38-1049 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 704.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   38 GLPVqPPGPV----PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGpLDVDALRAALDAVTARHEALRTVF-RLGPDGEP 112
Cdd:PRK12316 4091 ALPL-PLGEIediyPLSPMQQGMLFHSLYEQEAGDYINQMRVDVQG-LDVERFRAAWQAALDRHDVLRSGFvWQGELGRP 4168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  113 VQQTAPAAPVALPVIDVT-EADLPQALRTAAEQP----FDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLA 187
Cdd:PRK12316 4169 LQVVHKQVSLPFAELDWRgRADLQAALDALAAAErergFDLQRAPLLRLVLVRTAEGRHHLIYTNHHILMDGWSNSQLLG 4248

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  188 DLARAYAGELPAGvAAPAFRDVAAW-QHGRLAAGElddqlRYWRQRLAGLPDLEIPGDRQRPAD-RDWRAHS-VRRELPA 264
Cdd:PRK12316 4249 EVLERYSGRPPAQ-PGGRYRDYIAWlQRQDAAASE-----AFWREQLAALDEPTRLAQAIARADlRSANGYGeHVRELDA 4322

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  265 ATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRG--RTDLESVVGLFAGMVPVRLDLTGRPSFDEV 342
Cdd:PRK12316 4323 TATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPaeLPGIEGQIGLFINTLPVIATPRAQQSVVEW 4402

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  343 LSRTVAGSRNDFAHPQIPFDrlvrELRRDRIAGSQLLVQAMAGTESTAVA----------LTFGEAAGTEQPVdvalakC 412
Cdd:PRK12316 4403 LQQVQRQNLALREHEHTPLY----EIQRWAGQGGEALFDSLLVFENYPVSealqqgapggLRFGEVTNHEQTN------Y 4472

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  413 DLDLSVlDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPAA- 491
Cdd:PRK12316 4473 PLTLAV-GLGETLSLQFSYDRGHFDAATIERLARHLTNLLEAMAEDPQRRLGELQLLEKAEQQRIVALWNRTDAGYPATr 4551

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  492 SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPI 571
Cdd:PRK12316 4552 CVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPL 4631

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  572 DPDFPVERIAYLLSDARPVLVVTDAATADRL-GPDDITGLVVLEETDTGGYPATEPPAVPAGHS-AYVIYTSGSTGRPKG 649
Cdd:PRK12316 4632 DPEYPRERLAYMMEDSGAALLLTQSHLLQRLpIPDGLASLALDRDEDWEGFPAHDPAVRLHPDNlAYVIYTSGSTGRPKG 4711

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  650 VVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADaDTARNPAALIDLAGRHRVTLAQA 729
Cdd:PRK12316 4712 VAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLINGASVVIRD-DSLWDPERLYAEIHEHRVTVLVF 4790

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  730 TPTLWQALV--PELSGPALAGIRVLVGGEALPAELARRLGDAGAEVT--NMYGPTETTIWSTSGpTSEDSIRRGS----I 801
Cdd:PRK12316 4791 PPVYLQQLAehAERDGEPPSLRVYCFGGEAVAQASYDLAWRALKPVYlfNGYGPTETTVTVLLW-KARDGDACGAaympI 4869

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  802 GVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLG 881
Cdd:PRK12316 4870 GTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFGAPGGRLYRTGDLARYRADGVIDYLG 4949

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  882 RTDHQVKLRGFRIELGEIETTLINAGPVRRaAAVVREDRPGDLRLVAYVIPDGPVAPDA----------LRTELSRTLPD 951
Cdd:PRK12316 4950 RVDHQVKIRGFRIELGEIEARLREHPAVRE-AVVIAQEGAVGKQLVGYVVPQDPALADAdeaqaelrdeLKAALRERLPE 5028

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTAR-PPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFR 1030
Cdd:PRK12316 5029 YMVPAHLVFLARMPLTPNGKLDRKALPQPDASLLQQAYvAPRSELEQQVAAIWAEVLQLERVGLDDNFFELGGHSLLAIQ 5108

                        1050
                  ....*....|....*....
gi 653678460 1031 LLARIRDEFGAGFSLRRLL 1049
Cdd:PRK12316 5109 VTSRIQLELGLELPLRELF 5127
PRK05691 PRK05691
peptide synthase; Validated
 46-1354 0e+00

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 703.47  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQTAPAAPV--- 122
Cdd:PRK05691 1728 PVPLSYSQQRMWFLWQMEPDSPAYNVGGMARLSGVLDVDRFEAALQALILRHETLRTTFP-SVDGVPVQQVAEDSGLrmd 1806

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  123 -----ALPViDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLL---LADLARAYA 194
Cdd:PRK05691 1807 wqdfsALPA-DARQQRLQQLADSEAHQPFDLERGPLLRACLVKAAEREHYFVLTLHHIVTEGWAMDIFareLGALYEAFL 1885

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  195 GELPAGVAAPA--FRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAVR 271
Cdd:PRK05691 1886 DDRESPLEPLPvqYLDYSVWQRQWLESGERQRQLDYWKAQLGNeHPLLELPADRPRPPVQSHRGELYRFDLSPELAARVR 1965

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  272 RLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSR 351
Cdd:PRK05691 1966 AFNAQRGLTLFMTMTATLAALLYRYSGQRDLRIGAPVANRIRPESEGLIGAFLNTQVLRCQLDGQMSVSELLEQVRQTVI 2045

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  352 NDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVALTfGEAAG--TEQPVDVALA-KCDLDLSVLDDGDRLAVT 428
Cdd:PRK05691 2046 EGQSHQDLPFDHLVEALQPPRSAAYNPLFQVMCNVQRWEFQQS-RQLAGmtVEYLVNDARAtKFDLNLEVTDLDGRLGCC 2124

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  429 LVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTA-RPLPAASLRERFQQWCRRTPGA 507
Cdd:PRK05691 2125 LTYSRDLFDEPRIARMAEHWQNLLEALLGDPQQRLAELPLLAAAEQQQLLDSLAGEAgEARLDQTLHGLFAAQAARTPQA 2204

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  508 VAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDA 587
Cdd:PRK05691 2205 PALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDS 2284

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  588 RPVLVVTDAATADRLG--PDDItGLVVLEETDTG--GYPATEPPAVP-AGHSAYVIYTSGSTGRPKGVVITRAALDNFLA 662
Cdd:PRK05691 2285 GIGLLLSDRALFEALGelPAGV-ARWCLEDDAAAlaAYSDAPLPFLSlPQHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQ 2363

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  663 AMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLAdADTARNPAALIDLAGRHRVTLAQATPT----LWQALV 738
Cdd:PRK05691 2364 AVIERFGMRADDCELHFYSINFDAASERLLVPLLCGARVVLR-AQGQWGAEEICQLIREQQVSILGFTPSygsqLAQWLA 2442

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  739 PElsGPALAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEdSIRRGSIGVPIDNT----QVYV 812
Cdd:PRK05691 2443 GQ--GEQLPVRMCITGGEALTGEHLQRIRQAfaPQLFFNAYGPTETVVMPLACLAPE-QLEEGAASVPIGRVvgarVAYI 2519

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:PRK05691 2520 LDADLALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFAADGGRLYRTGDLVRLRADGLVEYVGRIDHQVKIRGF 2599

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLINAGPVRRAAaVVREDRPGDLRLVAYVIPDGPVAPD--------ALRTELSRTLPDYMIPAVIVPVPDF 964
Cdd:PRK05691 2600 RIELGEIESRLLEHPAVREAV-VLALDTPSGKQLAGYLVSAVAGQDDeaqaalreALKAHLKQQLPDYMVPAHLILLDSL 2678

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  965 PTTPNGKLDRAALPAPDYG-TRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRdEFGAGF 1043
Cdd:PRK05691 2679 PLTANGKLDRRALPAPDPElNRQAYQAPRSELEQQLAQIWREVLNVERVGLGDNFFELGGDSILSIQVVSRAR-QLGIHF 2757

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1044 SLRRLLAAPTVAAVAAelaaeTSTGDDAVHLAPVPRDEPLPLSPMQEsiWLADQVSAGDSVYNVPLALRLIGPLDRGALR 1123
Cdd:PRK05691 2758 SPRDLFQHQTVQTLAA-----VATHSEAAQAEQGPLQGASGLTPIQH--WFFDSPVPQPQHWNQALLLEPRQALDPALLE 2830

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1124 RTLNRVVDRHEMLRTRIHpGPDGMpVQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDERE 1203
Cdd:PRK05691 2831 QALQALVEHHDALRLRFS-QADGR-WQAEYRAVTAQELLWQVTVADFAECAALFADAQRSLDLQQGPLLRALLVDGPQGQ 2908

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1204 HVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELtGAVATE 1283
Cdd:PRK05691 2909 QRLLLAIHHLVVDGVSWRVLLEDLQALYRQLSAGAEPALPAKTSAFRDWAARLQAYAGSESLREELGWWQAQL-GGPRAE 2987

                        1290      1300      1310      1320      1330      1340      1350
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1284 VAGDLPRPATPGGGGGAVEFALAPRQIQGIRELARRC-GTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR 1354
Cdd:PRK05691 2988 LPCDRPQGGNLNRHAQTVSVRLDAERTRQLLQQAPAAyRTQVNDLLLTALARVLCRWSGQPSVLVQLEGHGR 3059
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
40-1040 0e+00

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 689.86  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   40 PVQPPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGpDGEPVQQTAPA 119
Cdd:PRK10252    1 AEPMSQHLPLVAAQPGIWMAEKLSPLPSAWSVAHYVELTGELDAPLLARAVVAGLAEADTLRMRFTED-NGEVWQWVDPA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  120 APVALP-VIDVTEADLPQALRTAA-----EQPFDLERG-PLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARA 192
Cdd:PRK10252   80 LTFPLPeIIDLRTQPDPHAAAQALmqadlQQDLRVDSGkPLVFHQLIQLGDNRWYWYQRYHHLLVDGFSFPAITRRIAAI 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  193 YA----GELPAGVAAPAFRDVAAwQHGRLAAGELDDQLR-YWRQRLAGLPDLEIPGDrQRPADRDWRAHSVRRELPAATE 267
Cdd:PRK10252  160 YCawlrGEPTPASPFTPFADVVE-EYQRYRASEAWQRDAaFWAEQRRQLPPPASLSP-APLPGRSASADILRLKLEFTDG 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  268 AAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRgrtdLESVVGLFAGMV----PVRLDLTGRPSFDEVL 343
Cdd:PRK10252  238 AFRQLAAQASGVQRPDLALALVALWLGRLCGRMDYAAGFIFMRR----LGSAALTATGPVlnvlPLRVHIAAQETLPELA 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  344 SRTVAGSRNDFAHPQIPFDRLVRELrrDRIAGSQLL------VQ------AMAGTESTAVALTFGeaagteqPVDvalak 411
Cdd:PRK10252  314 TRLAAQLKKMRRHQRYDAEQIVRDS--GRAAGDEPLfgpvlnIKvfdyqlDFPGVQAQTHTLATG-------PVN----- 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  412 cDLDLSVLDDGD-RLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDElHTVLCRWNDTARPLPA 490
Cdd:PRK10252  380 -DLELALFPDEHgGLSIEILANPQRYDEATLIAHAERLKALIAQFAADPALLCGDVDILLPGE-YAQLAQVNATAVEIPE 457

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  491 ASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLP 570
Cdd:PRK10252  458 TTLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLP 537

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  571 IDPDFPVERIAYLLSDARPVLVVTDAATADRLgpDDITGLVVLEETDTGGYPATEPPAVPA-GHSAYVIYTSGSTGRPKG 649
Cdd:PRK10252  538 LDTGYPDDRLKMMLEDARPSLLITTADQLPRF--ADVPDLTSLCYNAPLAPQGAAPLQLSQpHHTAYIIFTSGSTGRPKG 615

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  650 VVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQA 729
Cdd:PRK10252  616 VMVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHF 695

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  730 TPTLWQALV----PELSGPALAGIR-VLVGGEALPAELARRL-GDAGAEVTNMYGPTETTI---WSTSGPTSEDSIRRGS 800
Cdd:PRK10252  696 VPSMLAAFVasltPEGARQSCASLRqVFCSGEALPADLCREWqQLTGAPLHNLYGPTEAAVdvsWYPAFGEELAAVRGSS 775

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  801 --IGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPpGTRMYRTGDLVRWSAAGDLE 878
Cdd:PRK10252  776 vpIGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAP-GERMYRTGDVARWLDDGAVE 854

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  879 YLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVR-----EDRPGDLR-LVAYVIPDGPVAPD--ALRTELSRTLP 950
Cdd:PRK10252  855 YLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqaAATGGDARqLVGYLVSQSGLPLDtsALQAQLRERLP 934

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  951 DYMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFR 1030
Cdd:PRK10252  935 PHMVPVVLLQLDQLPLSANGKLDRKALPLPELKAQVPGRAPKTGTETIIAAAFSSLLGCDVVDADADFFALGGHSLLAMK 1014

                        1050
                  ....*....|
gi 653678460 1031 LLARIRDEFG 1040
Cdd:PRK10252 1015 LAAQLSRQFA 1024
PRK12467 PRK12467
peptide synthase; Provisional
 39-1290 0e+00

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 685.35  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   39 LPVqPPGPV----PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGpLDVDALRAALDAVTARHEALRTVF-RLGPDGEPV 113
Cdd:PRK12467 2636 LPV-AVGDIediyPLSPMQQGMLFHTLYEGGAGDYINQMRVDVEG-LDVERFRTAWQAVIDRHEILRSGFlWDGELEEPL 2713

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  114 QQTAPAAPVALPVIDVTE-ADLPQALRTAAE----QPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLAD 188
Cdd:PRK12467 2714 QVVYKQARLPFSRLDWRDrADLEQALDALAAadrqQGFDLLSAPLLRLTLVRTGEDRHHLIYTNHHILMDGWSGSQLLGE 2793

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  189 LARAYAGELPAGVAApAFRDVAAWqhgrLAAGELDDQLRYWRQRLAGL--PDLEIPGDRQRPADRDWRAHSVRRELPAAT 266
Cdd:PRK12467 2794 VLQRYFGQPPPAREG-RYRDYIAW----LQAQDAEASEAFWKEQLAALeePTRLARALYPAPAEAVAGHGAHYLHLDATQ 2868

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  267 EAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR--GRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLS 344
Cdd:PRK12467 2869 TRQLIEFARRHRVTLNTLVQGAWLLLLQRFTGQDTVCFGATVAGRpaQLRGAEQQLGLFINTLPVIASPRAEQTVSDWLQ 2948

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  345 RTVAGSRNDFAHPQIPfdrlVRELRRDRIAGSQLLVQAMAGTESTAVA----------LTFGEAAGTEQ---PVDVALAK 411
Cdd:PRK12467 2949 QVQAQNLALREFEHTP----LADIQRWAGQGGEALFDSILVFENYPISealkqgapsgLRFGAVSSREQtnyPLTLAVGL 3024

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  412 cdldlsvlddGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTARPLPA- 490
Cdd:PRK12467 3025 ----------GDTLELEFSYDRQHFDAAAIERLAESFDRLLQAMLNNPAARLGELPTLAAHERRQVLHAWNATAAAYPSe 3094

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  491 ASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLP 570
Cdd:PRK12467 3095 RLVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVP 3174

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  571 IDPDFPVERIAYLLSDARPVLVVTDAATADRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHS-AYVIYTSGSTGRPKG 649
Cdd:PRK12467 3175 LDPEYPRERLAYMIEDSGVKLLLTQAHLLEQLPAPAGDTALTLDRLDLNGYSENNPSTRVMGENlAYVIYTSGSTGKPKG 3254

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  650 VVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTaRNPAALIDLAGRHRVTLAQA 729
Cdd:PRK12467 3255 VGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLWTLICGGCLVVRDNDL-WDPEELWQAIHAHRISIACF 3333

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  730 TPTLWQALVPELSGPALAGI-RVLVGGEALP----AELARRLGDAGaeVTNMYGPTETTIWSTSGPTSEDSIRRGS---I 801
Cdd:PRK12467 3334 PPAYLQQFAEDAGGADCASLdIYVFGGEAVPpaafEQVKRKLKPRG--LTNGYGPTEAVVTVTLWKCGGDAVCEAPyapI 3411

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  802 GVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLG 881
Cdd:PRK12467 3412 GRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFSGSGGRLYRTGDLARYRADGVIEYLG 3491

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  882 RTDHQVKLRGFRIELGEIETTLINAGPVRRaAAVVREDRPGDLRLVAYVIPDGPVAP--DALRTELSRTLPDYMIPAVIV 959
Cdd:PRK12467 3492 RIDHQVKIRGFRIELGEIEARLLQHPSVRE-AVVLARDGAGGKQLVAYVVPADPQGDwrETLRDHLAASLPDYMVPAQLL 3570

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  960 PVPDFPTTPNGKLDRAALPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEF 1039
Cdd:PRK12467 3571 VLAAMPLGPNGKVDRKALPDPDAKGSREYVAPRSEVEQQLAAIWADVLGVEQVGVTDNFFELGGDSLLALQVLSRIRQSL 3650

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1040 GAGFSLRRLLaaptvaavaaelaaetstgddavhLAPVPRDeplplspmqesiwLADQVSAGDsvynvpLALRLIGPLDR 1119
Cdd:PRK12467 3651 GLKLSLRDLM------------------------SAPTIAE-------------LAGYSPLGD------VPVNLLLDLNR 3687

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1120 gaLRRTLNRVVDRHEMLRTrihpGPDGMPVQVAdpagggaalterdaapgtdlaqlllaeaalgftlatehplravlwrL 1199
Cdd:PRK12467 3688 --LETGFPALFCRHEGLGT----VFDYEPLAVI----------------------------------------------L 3715

                        1210      1220      1230      1240      1250      1260      1270      1280
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1200 DEREHVLLLTAHHVAVDAWSmdliqrdltelyaadtghrEPELDEPALAQADYAVWQRQSAQRGRYAAELDfwrteltGA 1279
Cdd:PRK12467 3716 EGDRHVLGLTCRHLLDDGWQ-------------------DTSLQAMAVQYADYILWQQAKGPYGLLGWSLG-------GT 3769

                        1290
                  ....*....|.
gi 653678460 1280 VATEVAGDLPR 1290
Cdd:PRK12467 3770 LARLVAELLER 3780
PRK12316 PRK12316
peptide synthase; Provisional
 1070-2113 0e+00

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 621.59  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1070 DAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGpLDRGALRRTLNRVVDRHEMLRTRI-HPGPDGMP 1148
Cdd:PRK12316 4090 DALPLPLGEIEDIYPLSPMQQGMLFHSLYEQEAGDYINQMRVDVQG-LDVERFRAAWQAALDRHDVLRSGFvWQGELGRP 4168

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1149 VQVADPAGGgAALTERDAAPGTDLAQLLLAEAAL----GFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQ 1224
Cdd:PRK12316 4169 LQVVHKQVS-LPFAELDWRGRADLQAALDALAAAererGFDLQRAPLLRLVLVRTAEGRHHLIYTNHHILMDGWSNSQLL 4247

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1225 RDLTELYAADTghrepeLDEPALAQADYAVW-QRQSAqrgryAAELDFWRTELTGAVA-TEVAGDLPRP-ATPGGGGGAV 1301
Cdd:PRK12316 4248 GEVLERYSGRP------PAQPGGRYRDYIAWlQRQDA-----AASEAFWREQLAALDEpTRLAQAIARAdLRSANGYGEH 4316

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1302 EFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGN--PRLDNLVGCLVNTVVLRGDLSGA 1379
Cdd:PRK12316 4317 VRELDATATARLREFARTQRVTLNTLVQAAWLLLLQRYTGQDTVAFGATVAGRPAelPGIEGQIGLFINTLPVIATPRAQ 4396

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1380 PTFHELLRRTHARTADALAHQELPFEHLVADRGAGNGPLFRIMYSFSS---------QEPAGRSAGDVDLEPVpvpvTTC 1450
Cdd:PRK12316 4397 QSVVEWLQQVQRQNLALREHEHTPLYEIQRWAGQGGEALFDSLLVFENypvsealqqGAPGGLRFGEVTNHEQ----TNY 4472

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1451 KFDLVltvVDGGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRT-SRWGRSEQHVGD 1529
Cdd:PRK12316 4473 PLTLA---VGLGETLSLQFSYDRGHFDAATIERLARHLTNLLEAMAEDPQRRLGELQLLEKAEQQRIvALWNRTDAGYPA 4549

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKA 1609
Cdd:PRK12316 4550 TRCVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALI--ARG---VGPEVLVGIAMERSAEMMVGLLAVLKA 4624

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1610 GGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfgDVPVVALDRvADRLTAMPDQRPEVTVDPRGLAYSI 1689
Cdd:PRK12316 4625 GGAYVPLDPEYPRERLAYMMEDSGAALLLTQSHLLQRLPIPD---GLASLALDR-DEDWEGFPAHDPAVRLHPDNLAYVI 4700

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 FTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADLAEELS 1769
Cdd:PRK12316 4701 YTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLINGASVVIRDDSLWDPERLYAEI 4780

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1770 GPLAYDYIRLTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVReAG 1849
Cdd:PRK12316 4781 HEHRVTVLVFPPVYLQQLAEHAERDGEPPSLRVYCFGGEAVAQASYDLAWRALKPVYLFNGYGPTETTVTVLLWKAR-DG 4859

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 ELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRW 1929
Cdd:PRK12316 4860 DACGAAYMPIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFGAPGGRLYRTGDLARY 4939

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPAEDH--PPVTVSA-----LR 1998
Cdd:PRK12316 4940 RADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEgavgKQLVGYVVPQDPAlaDADEAQAelrdeLK 5019

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1999 RFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGrpELEDRYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFF 2078
Cdd:PRK12316 5020 AALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDAS--LLQQAYVAPRSELEQQVAAIWAEVLQLERVGLDDNFF 5097

                        1050      1060      1070
                  ....*....|....*....|....*....|....*.
gi 653678460 2079 DLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVR 2113
Cdd:PRK12316 5098 ELGGHSLLAIQVTSRIQLElGLELPLRELFQTPTLA 5133
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 505-977 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 602.21  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAatadrlgpdditglvvleetdtggypateppavpaGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd05930    81 EDSGAKLVLTDP-----------------------------------DDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLWM 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGP 744
Cdd:cd05930   126 QEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGIR-VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQVYVLDANLHP 819
Cdd:cd05930   206 ALPSLRlVLVGGEALPPDLVRRWRELlpGARLVNLYGPTEATVDATYYRVPPDDEEDGRvpIGRPIPNTRVYVLDENLRP 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  820 APIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEI 899
Cdd:cd05930   286 VPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFG-PGERMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEI 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  900 ETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05930   365 EAALLAHPGVREAAVVAREDGDGEKRLVAYVVPDEGGELDeeELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
 505-977 0e+00

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 587.72  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd12116     1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLgPDDITGLVVLEETDTGGYPATEPPAVPaGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd12116    81 EDAEPALVLTDDALPDRL-PAGLPVLLLALAAAAAAPAAPRTPVSP-DDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHSM 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPelSG- 743
Cdd:cd12116   159 RERLGLGPGDRLLAVTTYAFDISLLELLLPLLAGARVVIAPRETQRDPEALARLIEAHSITVMQATPATWRMLLD--AGw 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  744 PALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTsEDSIRRGSIGVPIDNTQVYVLDANLHPAPIG 823
Cdd:cd12116   237 QGRAGLTALCGGEALPPDLAARLLSRVGSLWNLYGPTETTIWSTAARV-TAAAGPIPIGRPLANTQVYVLDAALRPVPPG 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  824 VLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTL 903
Cdd:cd12116   316 VPGELYIGGDGVAQGYLGRPALTAERFVPDPFAGPGSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAAL 395

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  904 INAGPVRRAAAVVREDRpGDLRLVAYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd12116   396 AAHPGVAQAAVVVREDG-GDRRLVAYVVLKAGAAPDaaALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
1076-2279 3.50e-178

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 583.93  E-value: 3.50e-178
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1076 PVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGmPVQVADPA 1155
Cdd:PRK10252    1 AEPMSQHLPLVAAQPGIWMAEKLSPLPSAWSVAHYVELTGELDAPLLARAVVAGLAEADTLRMRFTEDNGE-VWQWVDPA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1156 GGGAALTERD----AAPGTDLAQLLLAEAALGFTLATEHPL-RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTEL 1230
Cdd:PRK10252   80 LTFPLPEIIDlrtqPDPHAAAQALMQADLQQDLRVDSGKPLvFHQLIQLGDNRWYWYQRYHHLLVDGFSFPAITRRIAAI 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1231 YAADTgHREPELDEP----ALAQADYAVWqRQSAQRGRYAAeldFWRTELTG--AVATEVAGDLP-RPATPGGGGGAVEF 1303
Cdd:PRK10252  160 YCAWL-RGEPTPASPftpfADVVEEYQRY-RASEAWQRDAA---FWAEQRRQlpPPASLSPAPLPgRSASADILRLKLEF 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1304 ALAprqiqGIRELARRCGTTVSTVVLSALQTL-LYRITGGADVVIGSTVSGR-GNPRLdNLVGCLVNTVVLRGDLSGAPT 1381
Cdd:PRK10252  235 TDG-----AFRQLAAQASGVQRPDLALALVALwLGRLCGRMDYAAGFIFMRRlGSAAL-TATGPVLNVLPLRVHIAAQET 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1382 FHELLRRTHARTADALAHQELPFEHLVADRG--AGN----GPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPvttckfDLV 1455
Cdd:PRK10252  309 LPELATRLAAQLKKMRRHQRYDAEQIVRDSGraAGDeplfGPVLNIKVFDYQLDFPGVQAQTHTLATGPVN------DLE 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1456 LTV---VDGGSTLEgvLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRTSRWGRSeQHVGDDRT 1532
Cdd:PRK10252  383 LALfpdEHGGLSIE--ILANPQRYDEATLIAHAERLKALIAQFAADPALLCGDVDILLPGEYAQLAQVNAT-AVEIPETT 459

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGY 1612
Cdd:PRK10252  460 LSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLR--ERG---VKPGDSVAVALPRSVFLTLALHAIVEAGAA 534

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1613 FIPVDPGYPMARLTRIADTVTPALVLTRTGQggclpgAEVFGDVPVVALDRVADRLTAmPDQRPEVTVDPRGLAYSIFTS 1692
Cdd:PRK10252  535 WLPLDTGYPDDRLKMMLEDARPSLLITTADQ------LPRFADVPDLTSLCYNAPLAP-QGAAPLQLSQPHHTAYIIFTS 607

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1693 GSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADA-----TLADL-AE 1766
Cdd:PRK10252  608 GSTGRPKGVMVGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAhrdplAMQQFfAE 687

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1767 ElsgplAYDYIRLTPSHLRHLVGHWTGQELPPAA---RGWVVGGETLDPALVKQLlELRPDAEVINHYGPTETVIGRVVH 1843
Cdd:PRK10252  688 Y-----GVTTTHFVPSMLAAFVASLTPEGARQSCaslRQVFCSGEALPADLCREW-QQLTGAPLHNLYGPTEAAVDVSWY 761

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1844 PV-REAGELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYR 1922
Cdd:PRK10252  762 PAfGEELAAVRGSSVPIGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFA-PGERMYR 840

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1923 TGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----------DQQLVGWFIPAEDHPP 1991
Cdd:PRK10252  841 TGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqaaatggdARQLVGYLVSQSGLPL 920

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1992 vTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP-----TSGRpeledryqAPRTPGEQLLAELWSTVL 2066
Cdd:PRK10252  921 -DTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRKALPLPelkaqVPGR--------APKTGTETIIAAAFSSLL 991

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2067 GTDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVRGQAmRAIARSRDDAYAPVLDtvfgaghtgttr 2145
Cdd:PRK10252  992 GCDVVDADADFFALGGHSLLAMKLAAQLSRQfARQVTPGQVMVASTVAKLA-TLLDAEEDESRRLGFG------------ 1058

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2146 TVVTLRgEGDGRPVFCVHPSgGGVAW-YLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYV 2224
Cdd:PRK10252 1059 TILPLR-EGDGPTLFCFHPA-SGFAWqFSVLSRYLDPQWSIYGIQSPRPDGPMQTATSLDEVCEAHLATLLEQQPHGPYH 1136

                        1210      1220      1230      1240      1250      1260      1270
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 2225 VVGWSLGGTIAFEMARQLDRLGEPVQLILLEPTLP----------------EVARTIDLHRPARDSYLRGA 2279
Cdd:PRK10252 1137 LLGYSLGGTLAQGIAARLRARGEEVAFLGLLDTWPpetqnwrekeangldpEVLAEIDREREAFLAAQQGS 1207
PRK12467 PRK12467
peptide synthase; Provisional
 1070-2264 3.27e-176

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 606.39  E-value: 3.27e-176
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1070 DAVHLAPVPRDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGpLDRGALRRTLNRVVDRHEMLRTR-IHPGPDGMP 1148
Cdd:PRK12467 2634 DRLPVAVGDIEDIYPLSPMQQGMLFHTLYEGGAGDYINQMRVDVEG-LDVERFRTAWQAVIDRHEILRSGfLWDGELEEP 2712

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1149 VQV-----------------ADPAGGGAALTERDAApgtdlaqlllaeaaLGFTLATEHPLRAVLWRLDEREHVLLLTAH 1211
Cdd:PRK12467 2713 LQVvykqarlpfsrldwrdrADLEQALDALAAADRQ--------------QGFDLLSAPLLRLTLVRTGEDRHHLIYTNH 2778

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1212 HVAVDAWSMDLIQRDLTELYAADTghrepeLDEPALAQADYAVW-QRQSAQRGRyaaelDFWRTELtGAVA--TEVAGDL 1288
Cdd:PRK12467 2779 HILMDGWSGSQLLGEVLQRYFGQP------PPAREGRYRDYIAWlQAQDAEASE-----AFWKEQL-AALEepTRLARAL 2846

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1289 -PRPATPGGGGGAVEFALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNPRLDNLVGC 1365
Cdd:PRK12467 2847 yPAPAEAVAGHGAHYLHLDATQTRQLIEFARRHRVTLNTLVQGAWLLLLQRFTGQDTVCFGATVAGRpaQLRGAEQQLGL 2926

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1366 LVNTVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLVADRGAGNGPLFRIMYSFSSQ--EPAGRSAGDVDLEPV 1443
Cdd:PRK12467 2927 FINTLPVIASPRAEQTVSDWLQQVQAQNLALREFEHTPLADIQRWAGQGGEALFDSILVFENYpiSEALKQGAPSGLRFG 3006

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1444 PVP---VTTCKFDLVLTVvdgGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPE--LAVTRLAITSESDTVRTS 1518
Cdd:PRK12467 3007 AVSsreQTNYPLTLAVGL---GDTLELEFSYDRQHFDAAAIERLAESFDRLLQAMLNNPAarLGELPTLAAHERRQVLHA 3083

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1519 rWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPE 1598
Cdd:PRK12467 3084 -WNATAAAYPSERLVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLI--AIG---VGPDVLVGVAVERSVE 3157

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1599 LVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVfgdVPVVALDRVAdrLTAMPDQRPEV 1678
Cdd:PRK12467 3158 MIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLTQAHLLEQLPAPAG---DTALTLDRLD--LNGYSENNPST 3232

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLmpAD 1758
Cdd:PRK12467 3233 RVMGENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLWTLICGGCLVV--RD 3310

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1759 ATLADLAEELSGPLAYDY--IRLTPSHLRHLVGHWTGQELPPAARgWVVGGETLDPALVKQLLELRPDAEVINHYGPTET 1836
Cdd:PRK12467 3311 NDLWDPEELWQAIHAHRIsiACFPPAYLQQFAEDAGGADCASLDI-YVFGGEAVPPAAFEQVKRKLKPRGLTNGYGPTEA 3389

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1837 VIgRVVHPVREAGELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEP 1916
Cdd:PRK12467 3390 VV-TVTLWKCGGDAVCEAPYAPIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFSGS 3468

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1917 GARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPaEDHPPV 1992
Cdd:PRK12467 3469 GGRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLARDgaggKQLVAYVVP-ADPQGD 3547

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1993 TVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSgrpELEDRYQAPRTPGEQLLAELWSTVLGTDRIG 2072
Cdd:PRK12467 3548 WRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDA---KGSREYVAPRSEVEQQLAAIWADVLGVEQVG 3624

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2073 IEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVRGQAmrAIARSRDDAYAPVLDTVfgaghtgttrtvvtlR 2151
Cdd:PRK12467 3625 VTDNFFELGGDSLLALQVLSRIRQSlGLKLSLRDLMSAPTIAELA--GYSPLGDVPVNLLLDLN---------------R 3687

                        1130      1140      1150      1160      1170      1180      1190      1200
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2152 GEGDGRPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLG 2231
Cdd:PRK12467 3688 LETGFPALFCRHEGLGTVFDYEPLAVILEGDRHVLGLTCRHLLDDGWQDTSLQAMAVQYADYILWQQAKGPYGLLGWSLG 3767

                        1210      1220      1230
                  ....*....|....*....|....*....|....
gi 653678460 2232 GTIAFEMARQLDRLGEPVQLI-LLEPTLPEVART 2264
Cdd:PRK12467 3768 GTLARLVAELLEREGESEAFLgLFDNTLPLPDEF 3801
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 495-977 3.44e-173

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 540.71  E-value: 3.44e-173
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:cd17646     2 ALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAATADRLGPDDitGLVVLEETDTGGYPATEPPAVPAGHS-AYVIYTSGSTGRPKGVVIT 653
Cdd:cd17646    82 YPADRLAYMLADAGPAVVLTTADLAARLPAGG--DVALLGDEALAAPPATPPLVPPRPDNlAYVIYTSGSTGRPKGVMVT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  654 RAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTL 733
Cdd:cd17646   160 HAGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCHFVPSM 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  734 WQALVPELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIRRG-SIGVPIDNTQV 810
Cdd:cd17646   240 LRVFLAEPAAGSCASLRrVFCSGEALPPELAARFLALpGAELHNLYGPTEAAIDVTHWPVRGPAETPSvPIGRPVPNTRL 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  811 YVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLR 890
Cdd:cd17646   320 YVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPFG-PGSRMYRTGDLARWRPDGALEFLGRSDDQVKIR 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  891 GFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDG---PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTT 967
Cdd:cd17646   399 GFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAgaaGPDTAALRAHLAERLPEYMVPAAFVVLDALPLT 478

                         490
                  ....*....|
gi 653678460  968 PNGKLDRAAL 977
Cdd:cd17646   479 ANGKLDRAAL 488
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
 495-981 5.45e-168

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 526.51  E-value: 5.45e-168
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:cd17655     1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAATADRLgpdDITGLVVLEETDTGGYPATE--PPAVPAGHSAYVIYTSGSTGRPKGVVI 652
Cdd:cd17655    81 YPEERIQYILEDSGADILLTQSHLQPPI---AFIGLIDLLDEDTIYHEESEnlEPVSKSDDLAYVIYTSGSTGKPKGVMI 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  653 TRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPT 732
Cdd:cd17655   158 EHRGVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDLTPA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  733 LWQALVPELSGPALAGIRVLVGGEALPAELARRLGD---AGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDN 807
Cdd:cd17655   238 HLKLLDAADDSEGLSLKHLIVGGEALSTELAKKIIElfgTNPTITNAYGPTETTVDASIYQYEPETDQQVSvpIGKPLGN 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  808 TQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQV 887
Cdd:cd17655   318 TRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPF-VPGERMYRTGDLARWLPDGNIEFLGRIDHQV 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  888 KLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTT 967
Cdd:cd17655   397 KIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELPVAQLREFLARELPDYMIPSYFIKLDEIPLT 476

                         490
                  ....*....|....
gi 653678460  968 PNGKLDRAALPAPD 981
Cdd:cd17655   477 PNGKVDRKALPEPD 490
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
 495-977 2.61e-167

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 524.07  E-value: 2.61e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:cd12117     1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAATADRLGPDdiTGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITR 654
Cdd:cd12117    81 LPAERLAFMLADAGAKVLLTDRSLAGRAGGL--EVAVVIDEALDAGPAGNPAVPVSPDDLAYVMYTSGSTGRPKGVAVTH 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  655 AALDNfLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLW 734
Cdd:cd12117   159 RGVVR-LVKNTNYVTLGPDDRVLQTSPLAFDASTFEIWGALLNGARLVLAPKGTLLDPDALGALIAEEGVTVLWLTAALF 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  735 QALVPELSGpALAGIR-VLVGGEALPAELARRLGDAGAE--VTNMYGPTETTIWSTSGPTSEDSIRRGS--IGVPIDNTQ 809
Cdd:cd12117   238 NQLADEDPE-CFAGLReLLTGGEVVSPPHVRRVLAACPGlrLVNGYGPTENTTFTTSHVVTELDEVAGSipIGRPIANTR 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  810 VYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGtRMYRTGDLVRWSAAGDLEYLGRTDHQVKL 889
Cdd:cd12117   317 VYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPFGPGE-RLYRTGDLARWLPDGRLEFLGRIDDQVKI 395

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  890 RGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPN 969
Cdd:cd12117   396 RGFRIELGEIEAALRAHPGVREAVVVVREDAGGDKRLVAYVVAEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTAN 475


                  ....*...
gi 653678460  970 GKLDRAAL 977
Cdd:cd12117   476 GKVDRRAL 483
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
 505-978 3.62e-167

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 521.81  E-value: 3.62e-167
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd17652     1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAatadrlgpdditglvvleetdtggypateppavpaGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd17652    81 ADARPALLLTTP-----------------------------------DNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQ 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvPELSGP 744
Cdd:cd17652   126 IAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVLAPAEELLPGEPLADLLREHRITHVTLPPAALAAL-PPDDLP 204

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGirVLVGGEALPAELARRLGDaGAEVTNMYGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTQVYVLDANLHPAPIGV 824
Cdd:cd17652   205 DLRT--LVVAGEACPAELVDRWAP-GRRMINAYGPTETTVCATMAGPLPGG-GVPPIGRPVPGTRVYVLDARLRPVPPGV 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  825 LGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLI 904
Cdd:cd17652   281 PGELYIAGAGLARGYLNRPGLTAERFVADPFGAPGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALT 360

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  905 NAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDA--LRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALP 978
Cdd:cd17652   361 EHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAaeLRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRALP 436
PRK12316 PRK12316
peptide synthase; Provisional
 1065-2189 5.33e-157

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 546.86  E-value: 5.33e-157
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1065 TSTGDDAVHLAPVPRDEPLPLSPMQESIW---LADQvSAGDSVYNvplaLRL-IGPLDRGALRRTLNRVVDRHEMLRTRI 1140
Cdd:PRK12316 1539 SQAQLDALPLPAGEIADIYPLSPMQQGMLfhsLYEQ-EAGDYINQ----LRVdVQGLDPDRFRAAWQATVDRHEILRSGF 1613

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1141 HPGPD-GMPVQVAD-----PAGGGAALTERDaaPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVA 1214
Cdd:PRK12316 1614 LWQDGlEQPLQVIHkqvelPFAELDWRGRED--LGQALDALAQAERQKGFDLTRAPLLRLVLVRTGEGRHHLIYTNHHIL 1691

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1215 VDAWSMDLIQRDLTELYAadtGHREPEldePALAQADYAVW-QRQSAqrgryAAELDFWRTELTGAVA-TEVAGDLPRPA 1292
Cdd:PRK12316 1692 MDGWSNAQLLGEVLQRYA---GQPVAA---PGGRYRDYIAWlQRQDA-----AASEAFWKEQLAALEEpTRLAQAARTED 1760

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1293 TPGGGGGAVEfALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGN--PRLDNLVGCLVNTV 1370
Cdd:PRK12316 1761 GQVGYGDHQQ-LLDPAQTRALAEFARAQKVTLNTLVQAAWLLLLQRYTGQETVAFGATVAGRPAelPGIEQQIGLFINTL 1839

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1371 VLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLVADRGAGNGPLFRIMYSFSS---------QEPAGRSAGDV-DL 1440
Cdd:PRK12316 1840 PVIAAPRPDQSVADWLQEVQALNLALREHEHTPLYDIQRWAGQGGEALFDSLLVFENypvaealkqGAPAGLVFGRVsNH 1919

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1441 EPVPVPVTtckfdLVLTVvdgGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRT-SR 1519
Cdd:PRK12316 1920 EQTNYPLT-----LAVTL---GETLSLQYSYDRGHFDAAAIERLDRHLLHLLEQMAEDAQAALGELALLDAGERQRIlAD 1991

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1520 WGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPEL 1599
Cdd:PRK12316 1992 WDRTPEAYPRGPGVHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLR--ARG---VGPEVRVAIAAERSFEL 2066

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1600 VVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPgaeVFGDVPVVALDRVADrLTAMPDQRPEVT 1679
Cdd:PRK12316 2067 VVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERLP---LPAGVARLPLDRDAE-WADYPDTAPAVQ 2142

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1680 VDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGrgVTLMPADA 1759
Cdd:PRK12316 2143 LAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHPLLNG--ARVLIRDD 2220

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1760 TLADlAEELsgplaYDYIR--------LTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHY 1831
Cdd:PRK12316 2221 ELWD-PEQL-----YDEMErhgvtildFPPVYLQQLAEHAERDGRPPAVRVYCFGGEAVPAASLRLAWEALRPVYLFNGY 2294

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETVIGRVVHPVREAGELAVDSPlPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:PRK12316 2295 GPTEAVVTPLLWKCRPQDPCGAAYV-PIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPD 2373

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 PAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPaE 1987
Cdd:PRK12316 2374 PFSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQDgasgKQLVAYVVP-D 2452

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1988 DHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGrpELEDRYQAPRTPGEQLLAELWSTVLG 2067
Cdd:PRK12316 2453 DAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVS--QLRQAYVAPQEGLEQRLAAIWQAVLK 2530

                        1050      1060      1070      1080      1090      1100      1110      1120
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2068 TDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVrgQAMRAIARSRDDAYAPVLDTVfgaghtgtTRT 2146
Cdd:PRK12316 2531 VEQVGLDDHFFELGGHSLLATQVVSRVRQDlGLEVPLRILFERPTL--AAFAASLESGQTSRAPVLQKV--------TRV 2600

                        1130      1140      1150      1160
                  ....*....|....*....|....*....|....*....|...
gi 653678460 2147 VVTLRGEGDGRPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQ 2189
Cdd:PRK12316 2601 QPLPLSHAQQRQWFLWQLEPESAAYHLPSALHLRGVLDQAALE 2643
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
 505-977 3.35e-156

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 491.44  E-value: 3.35e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd17643     1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd17643    81 ADSGPSLLLTD--------PDDL---------------------------AYVIYTSGSTGRPKGVVVSHANVLALFAAT 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPEL--S 742
Cdd:cd17643   126 QRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSAFYQLVEAAdrD 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  743 GPALAGIR-VLVGGEALPAELAR----RLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRG---SIGVPIDNTQVYVLD 814
Cdd:cd17643   206 GRDPLALRyVIFGGEALEAAMLRpwagRFGLDRPQLVNMYGITETTVHVTFRPLDAADLPAAaasPIGRPLPGLRVYVLD 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  815 ANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRI 894
Cdd:cd17643   286 ADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFGGPGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRI 365

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  895 ELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:cd17643   366 ELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAAADiaELRALLKELLPDYMVPARYVPLDALPLTVNGKL 445


                  ....*
gi 653678460  973 DRAAL 977
Cdd:cd17643   446 DRAAL 450
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
 1545-2033 4.88e-156

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 490.89  E-value: 4.88e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd05930     1 PDAVAVVDGDQSLTYAELDARANRLARYLR--ERG---VGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAER 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd05930    76 LAYILEDSGAKLVLT-----------------------------------------DPDDLAYVIYTSGSTGKPKGVMVE 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADAT-----LADLAEELSgplaYDYIRL 1779
Cdd:cd05930   115 HRGLVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRkdpeaLADLLAEEG----ITVLHL 190

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1780 TPSHLRHLVGHwTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELavDSPLPL 1859
Cdd:cd05930   191 TPSLLRLLLQE-LELAALPSLRLVLVGGEALPPDLVRRWRELLPGARLVNLYGPTEATVDATYYRVPPDDEE--DGRVPI 267

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1860 GRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTGDLVRWRGDGLLDFVG 1939
Cdd:cd05930   268 GRPIPNTRVYVLDENLRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFG-PGERMYRTGDLVRWLPDGNLEFLG 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1940 RVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEDhPPVTVSALRRFCAEQLPEFMVPNQW 2014
Cdd:cd05930   347 RIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVARedgdgEKRLVAYVVPDEG-GELDEEELRAHLAERLPDYMVPSAF 425

                         490
                  ....*....|....*....
gi 653678460 2015 VALDAFPLTPHGKVNHRAL 2033
Cdd:cd05930   426 VVLDALPLTPNGKVDRKAL 444
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
 497-978 6.17e-153

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 484.15  E-value: 6.17e-153
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  497 FQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFP 576
Cdd:cd17651     1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  577 VERIAYLLSDARPVLVVTDAATADRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAA 656
Cdd:cd17651    81 AERLAFMLADAGPVLVLTHPALAGELAVELVAVTLLDQPGAAAGADAEPDPALDADDLAYVIYTSGSTGRPKGVVMPHRS 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  657 LDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQA 736
Cdd:cd17651   161 LANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIFSTLCAGATLVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALRA 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  737 LVPEL--SGPALAGIR-VLVGGEALP-----AELARRLGdaGAEVTNMYGPTETTIwSTSGPTSEDSIRRG---SIGVPI 805
Cdd:cd17651   241 LAEHGrpLGVRLAALRyLLTGGEQLVltedlREFCAGLP--GLRLHNHYGPTETHV-VTALSLPGDPAAWPappPIGRPI 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  806 DNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDH 885
Cdd:cd17651   318 DNTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPFV-PGARMYRTGDLARWLPDGELEFLGRADD 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  886 QVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPD--GPVAPDALRTELSRTLPDYMIPAVIVPVPD 963
Cdd:cd17651   397 QVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEKRLVAYVVGDpeAPVDAAELRAALATHLPEYMVPSAFVLLDA 476

                         490
                  ....*....|....*
gi 653678460  964 FPTTPNGKLDRAALP 978
Cdd:cd17651   477 LPLTPNGKLDRRALP 491
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 518-915 1.16e-147

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 465.59  E-value: 1.16e-147
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   518 SYRELDERANRLAR-LLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:TIGR01733    1 TYRELDERANRLARhLRAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   597 ATADRLGPDDITGLVV--LEETDTGGYPATEPPAVPAGHS--AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTA 672
Cdd:TIGR01733   81 ALASRLAGLVLPVILLdpLELAALDDAPAPPPPDAPSGPDdlAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   673 EDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHR-VTLAQATPTLWQALVPELsGPALAGIR- 750
Cdd:TIGR01733  161 DDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDAALLAALIAEHpVTVLNLTPSLLALLAAAL-PPALASLRl 239

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   751 VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS---IGVPIDNTQVYVLDANLHPAPIGVL 825
Cdd:TIGR01733  240 VILGGEALTPALVDRWRARgpGARLINLYGPTETTVWSTATLVDPDDAPRESpvpIGRPLANTRLYVLDDDLRPVPVGVV 319

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   826 GELHIAGEGLARGYWNRPGLTAEKFLPDPF-GPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLi 904
Cdd:TIGR01733  320 GELYIGGPGVARGYLNRPELTAERFVPDPFaGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAAL- 398

                          410
                   ....*....|.
gi 653678460   905 NAGPVRRAAAV 915
Cdd:TIGR01733  399 LRHPGVREAVV 409
LCL_NRPS-like cd19531
LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar ...
 46-459 1.03e-143

LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar domains including the C-domain of SgcC5, a free-standing NRPS with both ester- and amide- bond forming activity; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. Streptomyces globisporus SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380454 [Multi-domain]  Cd Length: 427  Bit Score: 454.89  E-value: 1.03e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGpDGEPVQQTAPAAPVALP 125
Cdd:cd19531     1 PLPLSFAQQRLWFLDQLEPGSAAYNIPGALRLRGPLDVAALERALNELVARHEALRTTFVEV-DGEPVQVILPPLPLPLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  126 VIDVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGEL- 197
Cdd:cd19531    80 VVDLSglpeaerEAEAQRLAREEARRPFDLARGPLLRATLLRLGEDEHVLLLTMHHIVSDGWSMGVLLRELAALYAAFLa 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  198 --PAGVAAPA--FRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEAAVRR 272
Cdd:cd19531   160 grPSPLPPLPiqYADYAVWQREWLQGEVLERQLAYWREQLAGAPPvLELPTDRPRPAVQSFRGARVRFTLPAELTAALRA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  273 LAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRN 352
Cdd:cd19531   240 LARREGATLFMTLLAAFQVLLHRYSGQDDIVVGTPVAGRNRAELEGLIGFFVNTLVLRTDLSGDPTFRELLARVRETALE 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  353 DFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTEST-AVALTFGEAAGTEQPVDVALAKCDLDLSVLDDGDRLAVTLVA 431
Cdd:cd19531   320 AYAHQDLPFEKLVEALQPERDLSRSPLFQVMFVLQNApAAALELPGLTVEPLEVDSGTAKFDLTLSLTETDGGLRGSLEY 399

                         410       420
                  ....*....|....*....|....*...
gi 653678460  432 AADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19531   400 NTDLFDAATIERMAGHFQTLLEAIVADP 427
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
 493-977 8.17e-143

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 453.31  E-value: 8.17e-143
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  493 LRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:cd12115     1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFPVERIAYLLSDARPVLVVTDAAtadrlgpdditglvvleetdtggypateppavpagHSAYVIYTSGSTGRPKGVVI 652
Cdd:cd12115    81 PAYPPERLRFILEDAQARLVLTDPD-----------------------------------DLAYVIYTSGSTGRPKGVAI 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  653 TRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADadtarNPAALIDLAGRHRVTLAQATPT 732
Cdd:cd12115   126 EHRNAAAFLQWAAAAFSAEELAGVLASTSICFDLSVFELFGPLATGGKVVLAD-----NVLALPDLPAAAEVTLINTVPS 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  733 LWQALVPElsGPALAGIRVL-VGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQ 809
Cdd:cd12115   201 AAAELLRH--DALPASVRVVnLAGEPLPRDLVQRLYARlqVERVVNLYGPSEDTTYSTVAPVPPGASGEVSIGRPLANTQ 278

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  810 VYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKL 889
Cdd:cd12115   279 AYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPFG-PGARLYRTGDLVRWRPDGLLEFLGRADNQVKV 357

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  890 RGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAP--DALRTELSRTLPDYMIPAVIVPVPDFPTT 967
Cdd:cd12115   358 RGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAGlvEDLRRHLGTRLPAYMVPSRFVRLDALPLT 437

                         490
                  ....*....|
gi 653678460  968 PNGKLDRAAL 977
Cdd:cd12115   438 PNGKIDRSAL 447
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
 505-978 8.87e-142

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 450.67  E-value: 8.87e-142
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd17649     1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDaatadrlGPDditglvvleetdtggypateppavpagHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd17649    81 EDSGAGLLLTH-------HPR---------------------------QLAYVIYTSGSTGTPKGVAVSHGPLAAHCQAT 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPEL--- 741
Cdd:cd17649   127 AERYGLTPGDRELQFASFNFDGAHEQLLPPLICGACVVLRPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEAdrt 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 -SGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS---IGVPIDNTQVYVLDANL 817
Cdd:cd17649   207 gDGRPPSLRLYIFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVWKCEAGAARAGAsmpIGRPLGGRSAYILDADL 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  818 HPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELG 897
Cdd:cd17649   287 NPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELG 366

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  898 EIETTLInAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVA----PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd17649   367 EIEAALL-EHPGVREAAVVALDGAGGKQLVAYVVLRAAAAqpelRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLD 445


                  ....*
gi 653678460  974 RAALP 978
Cdd:cd17649   446 RKALP 450
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
 1534-2033 2.38e-141

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 450.96  E-value: 2.38e-141
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1534 HQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:cd17646     1 HALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLL--RARG---VGPEDRVAVLLPRSADLVVALLAVLKAGAAY 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAevfGDVPVValdrVADRLTAMPDQRPEVTVDPRGLAYSIFTSG 1693
Cdd:cd17646    76 LPLDPGYPADRLAYMLADAGPAVVLTTADLAARLPAG---GDVALL----GDEALAAPPATPPLVPPRPDNLAYVIYTSG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1694 STGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPAD-----ATLADLAEEL 1768
Cdd:cd17646   149 STGRPKGVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGghrdpAYLAALIREH 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1769 SGPLAYdyirLTPSHLRHLVGhWTGQELPPAARGWVVGGETLDPALVKQLLELrPDAEVINHYGPTETVIGRVVHPVREA 1848
Cdd:cd17646   229 GVTTCH----FVPSMLRVFLA-EPAAGSCASLRRVFCSGEALPPELAARFLAL-PGAELHNLYGPTEAAIDVTHWPVRGP 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1849 GElavDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTGDLVR 1928
Cdd:cd17646   303 AE---TPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPFG-PGSRMYRTGDLAR 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1929 WRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-----QQLVGWFIPAEDHPPVTVSALRRFCAE 2003
Cdd:cd17646   379 WRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAapagaARLVGYVVPAAGAAGPDTAALRAHLAE 458

                         490       500       510
                  ....*....|....*....|....*....|
gi 653678460 2004 QLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17646   459 RLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
 492-978 3.13e-139

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 443.80  E-value: 3.13e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  492 SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPI 571
Cdd:cd17644     1 CIHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  572 DPDFPVERIAYLLSDARPVLVVTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVV 651
Cdd:cd17644    81 DPNYPQERLTYILEDAQISVLLTQ--------PENL---------------------------AYVIYTSGSTGKPKGVM 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATP 731
Cdd:cd17644   126 IEHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVTLLSGATLVLRPEEMRSSLEDFVQYIQQWQLTVLSLPP 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQALVPELSGPALAGIR----VLVGGEALPAELARRLGDAGAE---VTNMYGPTETTIWST----SGPTSEDsIRRGS 800
Cdd:cd17644   206 AYWHLLVLELLLSTIDLPSslrlVIVGGEAVQPELVRQWQKNVGNfiqLINVYGPTEATIAATvcrlTQLTERN-ITSVP 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  801 IGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPF-GPPGTRMYRTGDLVRWSAAGDLEY 879
Cdd:cd17644   285 IGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFnSSESERLYKTGDLARYLPDGNIEY 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  880 LGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDA--LRTELSRTLPDYMIPAV 957
Cdd:cd17644   365 LGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPSTveLRQFLKAKLPDYMIPSA 444

                         490       500
                  ....*....|....*....|.
gi 653678460  958 IVPVPDFPTTPNGKLDRAALP 978
Cdd:cd17644   445 FVVLEELPLTPNGKIDRRALP 465
PRK05691 PRK05691
peptide synthase; Validated
 38-1046 8.87e-135

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 475.43  E-value: 8.87e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   38 GLPVqPPGPV----PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVF--RLGPDGE 111
Cdd:PRK05691 3246 ALPV-PAAEIedvyPLTPMQEGLLLHTLLEPGTGLYYMQDRYRINSALDPERFAQAWQAVVARHEALRASFswNAGETML 3324

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  112 PVQQTAPAAPV------ALPViDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLL 185
Cdd:PRK05691 3325 QVIHKPGRTPIdyldwrGLPE-DGQEQRLQALHKQEREAGFDLLNQPPFHLRLIRVDEARYWFMMSNHHILIDAWCRSLL 3403

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  186 LADLARAYA----GELPAGVAAPAFRDVAAWqhgrLAAGELDDQLRYWRQRLAGLP-DLEIPGDRqrPADRDWRAHS--- 257
Cdd:PRK05691 3404 MNDFFEIYTalgeGREAQLPVPPRYRDYIGW----LQRQDLAQARQWWQDNLRGFErPTPIPSDR--PFLREHAGDSggm 3477

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  258 ----VRRELPAATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR--GRTDLESVVGLFAGMVPVRL 331
Cdd:PRK05691 3478 vvgdCYTRLDAADGARLRELAQAHQLTVNTFAQAAWALVLRRYSGDRDVLFGVTVAGRpvSMPQMQRTVGLFINSIALRV 3557

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  332 DLTG---RPSFDEVLSRTVAGSRNDFAHPQIPfdrLVRELRRDRIAGSQLLVQAMAGTESTAVALT-FGEAAGTEQPVDV 407
Cdd:PRK05691 3558 QLPAagqRCSVRQWLQGLLDSNMELREYEYLP---LVAIQECSELPKGQPLFDSLFVFENAPVEVSvLDRAQSLNASSDS 3634

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  408 ALAKCDLDLS-VLDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDELHTVLCRWNDTAR 486
Cdd:PRK05691 3635 GRTHTNFPLTaVCYPGDDLGLHLSYDQRYFDAPTVERLLGEFKRLLLALVQGFHGDLSELPLLGEQERDFLLDGCNRSER 3714

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  487 PLP-AASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAG 565
Cdd:PRK05691 3715 DYPlEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAG 3794

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  566 AAYLPIDPDFPVERIAYLLSDAR-PVLVVTDAATA------DRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHS-AYV 637
Cdd:PRK05691 3795 AGYLPLDPGLPAQRLQRIIELSRtPVLVCSAACREqarallDELGCANRPRLLVWEEVQAGEVASHNPGIYSGPDNlAYV 3874

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  638 IYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLE-LYLPLrSGAGVVLADADTARNPAALI 716
Cdd:PRK05691 3875 IYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQfLAAPL-FGARVEIVPNAIAHDPQGLL 3953

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  717 DLAGRHRVTLAQATPTLWQALVPElSGPALAGIR-VLVGGEALPAELAR----RLGDAGaeVTNMYGPTETTIWSTSGPT 791
Cdd:PRK05691 3954 AHVQAQGITVLESVPSLIQGMLAE-DRQALDGLRwMLPTGEAMPPELARqwlqRYPQIG--LVNAYGPAECSDDVAFFRV 4030

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 SEDSIRrGS---IGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDL 868
Cdd:PRK05691 4031 DLASTR-GSylpIGSPTDNNRLYLLDEALELVPLGAVGELCVAGTGVGRGYVGDPLRTALAFVPHPFGAPGERLYRTGDL 4109

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  869 VRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDlRLVAYVIP-DGPVAPDALRTE--- 944
Cdd:PRK05691 4110 ARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQEGVNGK-HLVGYLVPhQTVLAQGALLERikq 4188

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  945 -LSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPDYG-TRSTA-RPPRDDRERFLCTAFAEVLGLPEVAVSDNFFEL 1021
Cdd:PRK05691 4189 rLRAELPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGqLQSQAyLAPRNELEQTLATIWADVLKVERVGVHDNFFEL 4268

                        1050      1060
                  ....*....|....*....|....*
gi 653678460 1022 GGHSLLAFRLLARIRDEFGAGFSLR 1046
Cdd:PRK05691 4269 GGHSLLATQIASRVQKALQRNVPLR 4293
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
 1535-2036 1.15e-134

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 431.75  E-value: 1.15e-134
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1535 QLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgalgAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFI 1614
Cdd:cd17655     1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLR-----EKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYL 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 PVDPGYPMARLTRIADTVTPALVLTrtgQGGCLPGAEVFGDVPVVALDRVADRltamPDQRPEVTVDPRGLAYSIFTSGS 1694
Cdd:cd17655    76 PIDPDYPEERIQYILEDSGADILLT---QSHLQPPIAFIGLIDLLDEDTIYHE----ESENLEPVSKSDDLAYVIYTSGS 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADatladlaEELSGPLAY 1774
Cdd:cd17655   149 TGKPKGVMIEHRGVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLLSGNTLYIVRKE-------TVLDGQALT 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1775 DYIR--------LTPSHLRHL--VGHWTGQELppaaRGWVVGGETLDPALVKQLLELRPDA-EVINHYGPTETVIGRVVH 1843
Cdd:cd17655   222 QYIRqnritiidLTPAHLKLLdaADDSEGLSL----KHLIVGGEALSTELAKKIIELFGTNpTITNAYGPTETTVDASIY 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1844 PVrEAGELAVDSPlPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAgEPGARMYRT 1923
Cdd:cd17655   298 QY-EPETDQQVSV-PIGKPLGNTRIYILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPF-VPGERMYRT 374

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1924 GDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-DQQ----LVGWFIPAEDhppVTVSALR 1998
Cdd:cd17655   375 GDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARkDEQgqnyLCAYIVSEKE---LPVAQLR 451

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 653678460 1999 RFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:cd17655   452 EFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKALPEP 489
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
 1533-2033 7.72e-132

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 421.73  E-value: 7.72e-132
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGY 1612
Cdd:cd12115     1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARL--RAAG---VGPESRVGVCLERTPDLVVALLAVLKAGAA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1613 FIPVDPGYPMARLTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTS 1692
Cdd:cd12115    76 YVPLDPAYPPERLRFILEDAQARLVLT-----------------------------------------DPDDLAYVIYTS 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1693 GSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADLAEELSGPL 1772
Cdd:cd12115   115 GSTGRPKGVAIEHRNAAAFLQWAAAAFSAEELAGVLASTSICFDLSVFELFGPLATGGKVVLADNVLALPDLPAAAEVTL 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 aydyIRLTPSHLRHLVGHwtgQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELA 1852
Cdd:cd12115   195 ----INTVPSAAAELLRH---DALPASVRVVNLAGEPLPRDLVQRLYARLQVERVVNLYGPSEDTTYSTVAPVPPGASGE 267

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1853 VdsplPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTGDLVRWRGD 1932
Cdd:cd12115   268 V----SIGRPLANTQAYVLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPFG-PGARLYRTGDLVRWRPD 342

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1933 GLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWfIPAEDHPPVTVSALRRFCAEQLPE 2007
Cdd:cd12115   343 GLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIgdaagERRLVAY-IVAEPGAAGLVEDLRRHLGTRLPA 421

                         490       500
                  ....*....|....*....|....*.
gi 653678460 2008 FMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd12115   422 YMVPSRFVRLDALPLTPNGKIDRSAL 447
LCL_NRPS-like cd19531
LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar ...
 1082-1499 1.61e-131

LCL-type Condensation (C) domain of non-ribosomal peptide synthetases(NRPSs) and similar domains including the C-domain of SgcC5, a free-standing NRPS with both ester- and amide- bond forming activity; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. Streptomyces globisporus SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380454 [Multi-domain]  Cd Length: 427  Bit Score: 420.22  E-value: 1.61e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGpDGMPVQVADPAGGgAAL 1161
Cdd:cd19531     1 PLPLSFAQQRLWFLDQLEPGSAAYNIPGALRLRGPLDVAALERALNELVARHEALRTTFVEV-DGEPVQVILPPLP-LPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERD------AAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADT 1235
Cdd:cd19531    79 PVVDlsglpeAEREAEAQRLAREEARRPFDLARGPLLRATLLRLGEDEHVLLLTMHHIVSDGWSMGVLLRELAALYAAFL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1236 GHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVAT-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIR 1314
Cdd:cd19531   159 AGRPSPLPPLPIQYADYAVWQREWLQGEVLERQLAYWREQLAGAPPVlELPTDRPRPAVQSFRGARVRFTLPAELTAALR 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1315 ELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTA 1394
Cdd:cd19531   239 ALARREGATLFMTLLAAFQVLLHRYSGQDDIVVGTPVAGRNRAELEGLIGFFVNTLVLRTDLSGDPTFRELLARVRETAL 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1395 DALAHQELPFEHLVA----DRGAGNGPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLE 1470
Cdd:cd19531   319 EAYAHQDLPFEKLVEalqpERDLSRSPLFQVMFVLQNAPAAALELPGLTVEPLEVDSGTAKFDLTLSLTETDGGLRGSLE 398

                         410       420
                  ....*....|....*....|....*....
gi 653678460 1471 YADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19531   399 YNTDLFDAATIERMAGHFQTLLEAIVADP 427
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
 505-977 3.47e-131

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 421.29  E-value: 3.47e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd12114     1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADrlgPDDITGLVVLEETDTGGYPATEPPAVPAGH-SAYVIYTSGSTGRPKGVVITRAALDNFLAA 663
Cdd:cd12114    81 ADAGARLVLTDGPDAQ---LDVAVFDVLILDLDALAAPAPPPPVDVAPDdLAYVIFTSGSTGTPKGVMISHRAALNTILD 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  664 MAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQAL--VPEL 741
Cdd:cd12114   158 INRRFAVGPDDRVLALSSLSFDLSVYDIFGALSAGATLVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLldVLEA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGPALAGIR-VLVGGEALPAELARRLGD--AGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSI--GVPIDNTQVYVLDAN 816
Cdd:cd12114   238 AQALLPSLRlVLLSGDWIPLDLPARLRAlaPDARLISLGGATEASIWSIYHPIDEVPPDWRSIpyGRPLANQRYRVLDPR 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  817 LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIEL 896
Cdd:cd12114   318 GRDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTH---PDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIEL 394

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  897 GEIETTLINAGPVRRAAAVVReDRPGDLRLVAYVIPDG---PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd12114   395 GEIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDNdgtPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVD 473


                  ....
gi 653678460  974 RAAL 977
Cdd:cd12114   474 RAAL 477
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
 1535-2033 3.70e-131

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 421.22  E-value: 3.70e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1535 QLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFI 1614
Cdd:cd12117     1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLR--AAG---VGPGDVVGVLAERSPELVVALLAVLKAGAAYV 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 PVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVFGDVpvvaldrvADRLTAMPDQRPEVTVDPRGLAYSIFTSGS 1694
Cdd:cd12117    76 PLDPELPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVI--------DEALDAGPAGNPAVPVSPDDLAYVMYTSGS 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEHIGIVRYLSWAAASYPTAGRRgTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATL--ADLAEELSGPL 1772
Cdd:cd12117   148 TGRPKGVAVTHRGVVRLVKNTNYVTLGPDDR-VLQTSPLAFDASTFEIWGALLNGARLVLAPKGTLLdpDALGALIAEEG 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 AyDYIRLTPSHLRHLVghwtgQELPPAARGW---VVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREag 1849
Cdd:cd12117   227 V-TVLWLTAALFNQLA-----DEDPECFAGLrelLTGGEVVSPPHVRRVLAACPGLRLVNGYGPTENTTFTTSHVVTE-- 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 ELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTGDLVRW 1929
Cdd:cd12117   299 LDEVAGSIPIGRPIANTRVYVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPFG-PGERLYRTGDLARW 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEdhpPVTVSALRRFCAEQ 2004
Cdd:cd12117   378 LPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVRedaggDKRLVAYVVAEG---ALDAAELRAFLRER 454

                         490       500
                  ....*....|....*....|....*....
gi 653678460 2005 LPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd12117   455 LPAYMVPAAFVVLDELPLTANGKVDRRAL 483
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
 505-977 9.12e-129

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 413.02  E-value: 9.12e-129
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd17650     1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAM 664
Cdd:cd17650    81 EDSGAKLLLTQ--------PEDL---------------------------AYVIYTSGTTGKPKGVMVEHRNVAHAAHAW 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  665 AERFPLTAED-RVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPEL-- 741
Cdd:cd17650   126 RREYELDSFPvRLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALIRPVMAYVyr 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGPALAGIRVL-VGGEALPAE----LARRLGdAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGS---IGVPIDNTQVYVL 813
Cdd:cd17650   206 NGLDLSAMRLLiVGSDGCKAQdfktLAARFG-QGMRIINSYGVTEATIDSTYYEEGRDPLGDSAnvpIGRPLPNTAMYVL 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  814 DANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPpGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFR 893
Cdd:cd17650   285 DERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFAP-GERMYRTGDLARWRADGNVELLGRVDHQVKIRGFR 363

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  894 IELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd17650   364 IELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYVVAAATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVD 443


                  ....
gi 653678460  974 RAAL 977
Cdd:cd17650   444 RRAL 447
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
 1558-1972 1.01e-128

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 411.27  E-value: 1.01e-128
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1558 TFAGLDAQANRLAHALHTGALgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALV 1637
Cdd:TIGR01733    1 TYRELDERANRLARHLRAAGG----VGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLL 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1638 LTRTGQGGCLPGAeVFGDVPVVALDRVADRlTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAA 1717
Cdd:TIGR01733   77 LTDSALASRLAGL-VLPVILLDPLELAALD-DAPAPPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLAR 154

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1718 SYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLAD--LAEELSGPLAYDYIRLTPSHLRHLVGHwtGQE 1795
Cdd:TIGR01733  155 RYGLDPDDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDaaLLAALIAEHPVTVLNLTPSLLALLAAA--LPP 232

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1796 LPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVrEAGELAVDSPLPLGRPLGETRLQVLDAWL 1875
Cdd:TIGR01733  233 ALASLRLVILGGEALTPALVDRWRARGPGARLINLYGPTETTVWSTATLV-DPDDAPRESPVPIGRPLANTRLYVLDDDL 311

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1876 EAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDP-AGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIEL 1954
Cdd:TIGR01733  312 RPVPVGVVGELYIGGPGVARGYLNRPELTAERFVPDPfAGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIEL 391

                          410
                   ....*....|....*...
gi 653678460  1955 GEIEARMAEHPGVEQAVV 1972
Cdd:TIGR01733  392 GEIEAALLRHPGVREAVV 409
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
 1537-2034 2.16e-128

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 413.66  E-value: 2.16e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1537 VEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV 1616
Cdd:cd17651     1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLR--ARG---VGPGDLVALCARRSAELVVALLAILKAGAAYVPL 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1617 DPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVfgdvPVVALDRVADrlTAMPDQRPEVTVDPRGLAYSIFTSGSTG 1696
Cdd:cd17651    76 DPAYPAERLAFMLADAGPVLVLTHPALAGELAVELV----AVTLLDQPGA--AAGADAEPDPALDADDLAYVIYTSGSTG 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1697 QPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPAD-----ATLADLAEELsgp 1771
Cdd:cd17651   150 RPKGVVMPHRSLANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIFSTLCAGATLVLPPEEvrtdpPALAAWLDEQ--- 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1772 lAYDYIRLTPSHLRHLVGH-WTGQELPPAARGWVVGGETLDP-ALVKQLLELRPDAEVINHYGPTETVigrvvhpVREAG 1849
Cdd:cd17651   227 -RISRVFLPTVALRALAEHgRPLGVRLAALRYLLTGGEQLVLtEDLREFCAGLPGLRLHNHYGPTETH-------VVTAL 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 ELAVD-----SPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTG 1924
Cdd:cd17651   299 SLPGDpaawpAPPPIGRPIDNTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPFV-PGARMYRTG 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1925 DLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEDHPPvTVSALRR 1999
Cdd:cd17651   378 DLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLARedrpgEKRLVAYVVGDPEAPV-DAAELRA 456

                         490       500       510
                  ....*....|....*....|....*....|....*
gi 653678460 2000 FCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17651   457 ALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
 495-977 8.20e-128

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 411.55  E-value: 8.20e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:cd05918     3 DLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLDPS 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITR 654
Cdd:cd05918    83 HPLQRLQEILQDTGAKVVLTSS-------PSDA---------------------------AYVIFTSGSTGKPKGVVIEH 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  655 AALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNpaaliDLAG---RHRVTLAQATP 731
Cdd:cd05918   129 RALSTSALAHGRALGLTSESRVLQFASYTFDVSILEIFTTLAAGGCLCIPSEEDRLN-----DLAGfinRLRVTWAFLTP 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQALVPElsgpALAGIRVLV-GGEALPAELARRLGDaGAEVTNMYGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTqV 810
Cdd:cd05918   204 SVARLLDPE----DVPSLRTLVlGGEALTQSDVDTWAD-RVRLINAYGPAECTIAATVSPVVPST-DPRNIGRPLGAT-C 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  811 YVLDANLH--PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPF------GPPGTRMYRTGDLVRWSAAGDLEYLGR 882
Cdd:cd05918   277 WVVDPDNHdrLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPAwlkqegSGRGRRLYRTGDLVRYNPDGSLEYVGR 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  883 TDHQVKLRGFRIELGEIETTLINAGPVRR---AAAVVREDRPGDLRLVAYVIPDG-------------------PVAPDA 940
Cdd:cd05918   357 KDTQVKIRGQRVELGEIEHHLRQSLPGAKevvVEVVKPKDGSSSPQLVAFVVLDGsssgsgdgdslflepsdefRALVAE 436

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 653678460  941 LRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05918   437 LRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
 1545-2033 9.20e-128

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 410.91  E-value: 9.20e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHTGAlgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd12116     1 PDATAVRDDDRSLSYAELDERANRLAARLRARG-----VGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADR 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTRTGqggcLPgAEVFGDVPVVALDRVADRLtamPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd12116    76 LRYILEDAEPALVLTDDA----LP-DRLPAGLPVLLLALAAAAA---APAAPRTPVSPDDLAYVIYTSGSTGRPKGVVVS 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYL-SWAAASYPTAGRRgTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATL--ADLAEELSGPLAyDYIRLTP 1781
Cdd:cd12116   148 HRNLVNFLhSMRERLGLGPGDR-LLAVTTYAFDISLLELLLPLLAGARVVIAPRETQRdpEALARLIEAHSI-TVMQATP 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1782 SHLRHLVGhwTGQELPPAARGwVVGGETLDPALVKQLLElrPDAEVINHYGPTETVIGRVVHPVREAgelavDSPLPLGR 1861
Cdd:cd12116   226 ATWRMLLD--AGWQGRAGLTA-LCGGEALPPDLAARLLS--RVGSLWNLYGPTETTIWSTAARVTAA-----AGPIPIGR 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1862 PLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRV 1941
Cdd:cd12116   296 PLANTQVYVLDAALRPVPPGVPGELYIGGDGVAQGYLGRPALTAERFVPDPFAGPGSRLYRTGDLVRRRADGRLEYLGRA 375

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1942 DDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR----DQQLVGWFIPAEDHPPvTVSALRRFCAEQLPEFMVPNQWVAL 2017
Cdd:cd12116   376 DGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVRedggDRRLVAYVVLKAGAAP-DAAALRAHLRATLPAYMVPSAFVRL 454

                         490
                  ....*....|....*.
gi 653678460 2018 DAFPLTPHGKVNHRAL 2033
Cdd:cd12116   455 DALPLTANGKLDRKAL 470
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
 1545-2034 4.93e-124

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 399.44  E-value: 4.93e-124
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd17649     1 PDAVALVFGDQSLSYAELDARANRLAHRLR--ALG---VGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAER 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTRtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd17649    76 LRYMLEDSGAGLLLTH----------------------------------------HPRQLAYVIYTSGSTGTPKGVAVS 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYP-TAGRRgTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADatLADLAEELSGPLAYDYIR---LT 1780
Cdd:cd17649   116 HGPLAAHCQATAERYGlTPGDR-ELQFASFNFDGAHEQLLPPLICGACVVLRPDE--LWASADELAEMVRELGVTvldLP 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1781 PSHLRHLVGHW--TGQELPPAARGWVVGGETLDPALVKQLLELrpDAEVINHYGPTETVIGRVVHPVReAGELAVDSPLP 1858
Cdd:cd17649   193 PAYLQQLAEEAdrTGDGRPPSLRLYIFGGEALSPELLRRWLKA--PVRLFNAYGPTEATVTPLVWKCE-AGAARAGASMP 269

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFV 1938
Cdd:cd17649   270 IGRPLGGRSAYILDADLNPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPFGAPGSRLYRTGDLARWRDDGVIEYL 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD----QQLVGWFIPAEDHP-PVTVSALRRFCAEQLPEFMVPNQ 2013
Cdd:cd17649   350 GRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDgaggKQLVAYVVLRAAAAqPELRAQLRTALRASLPDYMVPAH 429

                         490       500
                  ....*....|....*....|.
gi 653678460 2014 WVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17649   430 LVFLARLPLTPNGKLDRKALP 450
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
 1545-2034 1.37e-123

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 397.78  E-value: 1.37e-123
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd17652     1 PDAPAVVFGDETLTYAELNARANRLARLLA--ARG---VGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAER 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd17652    76 IAYMLADARPALLLT-----------------------------------------TPDNLAYVIYTSGSTGRPKGVVVT 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLA--DLAEELsGPLAYDYIRLTPS 1782
Cdd:cd17652   115 HRGLANLAAAQIAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVLAPAEELLPgePLADLL-REHRITHVTLPPA 193

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1783 HLRHLvghwTGQELPPAaRGWVVGGETLDPALVKQLlelRPDAEVINHYGPTETVIGRVVHPVREAGelavdSPLPLGRP 1862
Cdd:cd17652   194 ALAAL----PPDDLPDL-RTLVVAGEACPAELVDRW---APGRRMINAYGPTETTVCATMAGPLPGG-----GVPPIGRP 260

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1863 LGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVD 1942
Cdd:cd17652   261 VPGTRVYVLDARLRPVPPGVPGELYIAGAGLARGYLNRPGLTAERFVADPFGAPGSRMYRTGDLARWRADGQLEFLGRAD 340

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1943 DQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-----QQLVGWFIPAEDhPPVTVSALRRFCAEQLPEFMVPNQWVAL 2017
Cdd:cd17652   341 DQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDdrpgdKRLVAYVVPAPG-AAPTAAELRAHLAERLPGYMVPAAFVVL 419

                         490
                  ....*....|....*..
gi 653678460 2018 DAFPLTPHGKVNHRALP 2034
Cdd:cd17652   420 DALPLTPNGKLDRRALP 436
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
 504-978 2.55e-122

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 395.69  E-value: 2.55e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  504 TPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYL 583
Cdd:cd17656     1 TPDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDAATADRLgpdDITGLVVLEETDTGGYPATE--PPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:cd17656    81 MLDSGVRVVLTQRHLKSKL---SFNKSTILLEDPSISQEDTSniDYINNSDDLLYIIYTSGTTGKPKGVQLEHKNMVNLL 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPE- 740
Cdd:cd17656   158 HFEREKTNINFSDKVLQFATCSFDVCYQEIFSTLLSGGTLYIIREETKRDVEQLFDLVKRHNIEVVFLPVAFLKFIFSEr 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  741 -LSGPALAGIR-VLVGGEAL--PAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRR-GSIGVPIDNTQVYVLDA 815
Cdd:cd17656   238 eFINRFPTCVKhIITAGEQLviTNEFKEMLHEHNVHLHNHYGPSETHVVTTYTINPEAEIPElPPIGKPISNTWIYILDQ 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  816 NLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIE 895
Cdd:cd17656   318 EQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPF-DPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIE 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  896 LGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRA 975
Cdd:cd17656   397 LGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFVMEQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRK 476


                  ...
gi 653678460  976 ALP 978
Cdd:cd17656   477 ALP 479
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
 497-978 7.62e-121

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 389.99  E-value: 7.62e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  497 FQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFP 576
Cdd:cd17645     4 FEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYP 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  577 VERIAYLLSDARPVLVVTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAA 656
Cdd:cd17645    84 GERIAYMLADSSAKILLTN--------PDDL---------------------------AYVIYTSGSTGLPKGVMIEHHN 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  657 LDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAqATPTLWQA 736
Cdd:cd17645   129 LVNLCEWHRPYFGVTPADKSLVYASFSFDASAWEIFPHLTAGAALHVVPSERRLDLDALNDYFNQEGITIS-FLPTGAAE 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  737 LVPELSGPALagiRVLV-GGEALpaelaRRLGDAGAEVTNMYGPTETTIWSTSGPT--SEDSIrrgSIGVPIDNTQVYVL 813
Cdd:cd17645   208 QFMQLDNQSL---RVLLtGGDKL-----KKIERKGYKLVNNYGPTENTVVATSFEIdkPYANI---PIGKPIDNTRVYIL 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  814 DANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFR 893
Cdd:cd17645   277 DEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPF-VPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYR 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  894 IELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd17645   356 IEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAYVTAPEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVD 435


                  ....*
gi 653678460  974 RAALP 978
Cdd:cd17645   436 RKALP 440
PRK05691 PRK05691
peptide synthase; Validated
 1076-2091 1.06e-117

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 420.34  E-value: 1.06e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1076 PVPR---DEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTR------------I 1140
Cdd:PRK05691 3248 PVPAaeiEDVYPLTPMQEGLLLHTLLEPGTGLYYMQDRYRINSALDPERFAQAWQAVVARHEALRASfswnagetmlqvI 3327

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1141 H-PGPDGMPVQ--VADPAGGGA----AL--TERDAapgtdlaqlllaeaalGFTLATEHPLRAVLWRLDEREHVLLLTAH 1211
Cdd:PRK05691 3328 HkPGRTPIDYLdwRGLPEDGQEqrlqALhkQEREA----------------GFDLLNQPPFHLRLIRVDEARYWFMMSNH 3391

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1212 HVAVDAWSMDLIQRDLTELYAADTGHREPELDEPALAQaDYAVW-QRQSAQRGRyaaelDFWRTELTG-AVATEVAGDLP 1289
Cdd:PRK05691 3392 HILIDAWCRSLLMNDFFEIYTALGEGREAQLPVPPRYR-DYIGWlQRQDLAQAR-----QWWQDNLRGfERPTPIPSDRP 3465

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1290 --RPATPGGGGGAVE---FALAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNPRLDNL 1362
Cdd:PRK05691 3466 flREHAGDSGGMVVGdcyTRLDAADGARLRELAQAHQLTVNTFAQAAWALVLRRYSGDRDVLFGVTVAGRpvSMPQMQRT 3545

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1363 VGCLVNTVVLRGDLSGAP---TFHELLRRTHARTADALAHQELPFEHLVADRGAGNG-PLFRIMYSFSSQepagrsagdv 1438
Cdd:PRK05691 3546 VGLFINSIALRVQLPAAGqrcSVRQWLQGLLDSNMELREYEYLPLVAIQECSELPKGqPLFDSLFVFENA---------- 3615

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1439 dlePVPVPVTTCKFDLVLTVvDGGST-----LEGVLEYADDLYL----------PGTAERLVTSLTNLLAAAVRTPELAV 1503
Cdd:PRK05691 3616 ---PVEVSVLDRAQSLNASS-DSGRThtnfpLTAVCYPGDDLGLhlsydqryfdAPTVERLLGEFKRLLLALVQGFHGDL 3691

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1504 TRLAITSESD-TVRTSRWGRSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGapp 1582
Cdd:PRK05691 3692 SELPLLGEQErDFLLDGCNRSERDYPLEQSYVRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALR--AAG--- 3766

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1583 VGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIAD-TVTPALVLTRTgqggCLP-GAEVFGDVPVVA 1660
Cdd:PRK05691 3767 VGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDPGLPAQRLQRIIElSRTPVLVCSAA----CREqARALLDELGCAN 3842

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1661 LDR--VADRLTA--MPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIV-RYLSwaAASYPTAGRRGTLAHS-SVG 1734
Cdd:PRK05691 3843 RPRllVWEEVQAgeVASHNPGIYSGPDNLAYVIYTSGSTGLPKGVMVEQRGMLnNQLS--KVPYLALSEADVIAQTaSQS 3920

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1735 FDLTM-SALFEPLVSGRgVTLMPadatlADLAEELSGPLAY---DYIRLTPSHLRHLVGHWTGQELPPAARGWVV-GGET 1809
Cdd:PRK05691 3921 FDISVwQFLAAPLFGAR-VEIVP-----NAIAHDPQGLLAHvqaQGITVLESVPSLIQGMLAEDRQALDGLRWMLpTGEA 3994

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1810 LDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELAvdSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIG 1889
Cdd:PRK05691 3995 MPPELARQWLQRYPQIGLVNAYGPAECSDDVAFFRVDLASTRG--SYLPIGSPTDNNRLYLLDEALELVPLGAVGELCVA 4072

                         890       900       910       920       930       940       950       960
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1890 GDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ 1969
Cdd:PRK05691 4073 GTGVGRGYVGDPLRTALAFVPHPFGAPGERLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVRE 4152

                         970       980       990      1000      1010      1020      1030      1040
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1970 AVVLVRD----QQLVGWFIPAEdhPPVTVSALRRFCAEQ----LPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRP 2041
Cdd:PRK05691 4153 AAVAVQEgvngKHLVGYLVPHQ--TVLAQGALLERIKQRlraeLPDYMVPLHWLWLDRLPLNANGKLDRKALPALDIGQL 4230

                        1050      1060      1070      1080      1090
                  ....*....|....*....|....*....|....*....|....*....|
gi 653678460 2042 ELEDrYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLS 2091
Cdd:PRK05691 4231 QSQA-YLAPRNELEQTLATIWADVLKVERVGVHDNFFELGGHSLLATQIA 4279
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
 505-978 9.22e-117

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 378.67  E-value: 9.22e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAG-AETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYL 583
Cdd:cd17648     1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAEIrPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAA 663
Cdd:cd17648    81 LEDTGARVVITN--------STDL---------------------------AYAIYTSGTTGKPKGVLVEHGSVVNLRTS 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  664 MAERFPLTAED--RVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvpEL 741
Cdd:cd17648   126 LSERYFGRDNGdeAVLFFSNYVFDFFVEQMTLALLNGQKLVVPPDEMRFDPDRFYAYINREKVTYLSGTPSVLQQY--DL 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SG-PALAgiRVLVGGEALPAELARRL-GDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHP 819
Cdd:cd17648   204 ARlPHLK--RVDAAGEEFTAPVFEKLrSRFAGLIINAYGPTETTVTNHKRFFPGDQRFDKSLGRPVRNTKCYVLNDAMKR 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  820 APIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPG-------TRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:cd17648   282 VPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTEQerargrnARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQ 361

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLINAGPVRRAAAVVRED-----RPGDLRLVAYVIPDGPVAPDA-LRTELSRTLPDYMIPAVIVPVPDFPT 966
Cdd:cd17648   362 RIEPGEVEAALASYPGVRECAVVAKEDasqaqSRIQKYLVGYYLPEPGHVPESdLLSFLRAKLPRYMVPARLVRLEGIPV 441

                         490
                  ....*....|..
gi 653678460  967 TPNGKLDRAALP 978
Cdd:cd17648   442 TINGKLDVRALP 453
LCL_NRPS-like cd19540
LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; ...
 46-459 1.19e-116

LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380463 [Multi-domain]  Cd Length: 433  Bit Score: 377.53  E-value: 1.19e-116
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGpDGEPVQQTAPA--APVA 123
Cdd:cd19540     1 RIPLSFAQQRLWFLNRLDGPSAAYNIPLALRLTGALDVDALRAALADVVARHESLRTVFPED-DGGPYQVVLPAaeARPD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  124 LPVIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY----AGELPA 199
Cdd:cd19540    80 LTVVDVTEDELAARLAEAARRGFDLTAELPLRARLFRLGPDEHVLVLVVHHIAADGWSMAPLARDLATAYaarrAGRAPD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  200 GVAAPA-FRDVAAWQHGRL-----AAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEAAVRR 272
Cdd:cd19540   160 WAPLPVqYADYALWQRELLgdeddPDSLAARQLAYWRETLAGLPEeLELPTDRPRPAVASYRGGTVEFTIDAELHARLAA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  273 LAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRN 352
Cdd:cd19540   240 LAREHGATLFMVLHAALAVLLSRLGAGDDIPIGTPVAGRGDEALDDLVGMFVNTLVLRTDVSGDPTFAELLARVRETDLA 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  353 DFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVA-LTFGEAAGTEQPVDVALAKCDLDLSVLDDGDR------L 425
Cdd:cd19540   320 AFAHQDVPFERLVEALNPPRSTARHPLFQVMLAFQNTAAAtLELPGLTVEPVPVDTGVAKFDLSFTLTERRDAdgapagL 399

                         410       420       430
                  ....*....|....*....|....*....|....
gi 653678460  426 AVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19540   400 TGELEYATDLFDRSTAERLADRFVRVLEAVVADP 433
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
 1532-2034 2.03e-116

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 378.32  E-value: 2.03e-116
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGG 1611
Cdd:cd17644     1 CIHQLFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQS--LG---VKSESLVGICVERSLEMIIGLLAILKAGG 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1612 YFIPVDPGYPMARLTRIADTVTPALVLTRtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvdPRGLAYSIFT 1691
Cdd:cd17644    76 AYVPLDPNYPQERLTYILEDAQISVLLTQ-----------------------------------------PENLAYVIYT 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1692 SGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPAD--ATLADLAeels 1769
Cdd:cd17644   115 SGSTGKPKGVMIEHQSLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVTLLSGATLVLRPEEmrSSLEDFV---- 190

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1770 gplayDYIR--------LTPSHLRHLV--GHWTGQELPPAARGWVVGGETLDPALVKQLLE-LRPDAEVINHYGPTETVI 1838
Cdd:cd17644   191 -----QYIQqwqltvlsLPPAYWHLLVleLLLSTIDLPSSLRLVIVGGEAVQPELVRQWQKnVGNFIQLINVYGPTEATI 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1839 GRVVHPVREAGELAVDSPlPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDP-AGEPG 1917
Cdd:cd17644   266 AATVCRLTQLTERNITSV-PIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPfNSSES 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1918 ARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEDHPPv 1992
Cdd:cd17644   345 ERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVRedqpgNKRLVAYIVPHYEESP- 423

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 653678460 1993 TVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17644   424 STVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRALP 465
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
 1545-2033 3.67e-116

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 377.03  E-value: 3.67e-116
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd17643     1 PEAVAVVDEDRRLTYGELDARANRLARTLR--AEG---VGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd17643    76 IAFILADSGPSLLLT-----------------------------------------DPDDLAYVIYTSGSTGRPKGVVVS 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYP-TAGRRGTLAHSSvGFDLTMSALFEPLVSGRGVTLMPADATLAdlAEELSGPLAYDYIRL---T 1780
Cdd:cd17643   115 HANVLALFAATQRWFGfNEDDVWTLFHSY-AFDFSVWEIWGALLHGGRLVVVPYEVARS--PEDFARLLRDEGVTVlnqT 191

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1781 PSHLRHLV-GHWTGQELPPAARGWVVGGETLDPALVKQLLELRPD--AEVINHYGPTETVIGRVVHPVREAGELAVDSPl 1857
Cdd:cd17643   192 PSAFYQLVeAADRDGRDPLALRYVIFGGEALEAAMLRPWAGRFGLdrPQLVNMYGITETTVHVTFRPLDAADLPAAAAS- 270

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1858 PLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDF 1937
Cdd:cd17643   271 PIGRPLPGLRVYVLDADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFGGPGSRMYRTGDLARRLPDGELEY 350

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1938 VGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEDHPPVTvSALRRFCAEQLPEFMVPN 2012
Cdd:cd17643   351 LGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVRedepgDTRLVAYVVADDGAAADI-AELRALLKELLPDYMVPA 429

                         490       500
                  ....*....|....*....|.
gi 653678460 2013 QWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17643   430 RYVPLDALPLTVNGKLDRAAL 450
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
 502-977 1.66e-114

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 371.96  E-value: 1.66e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:cd05945     2 AANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARPVLVVTDaatadrlgPDDitglvvleetdtggypateppavpaghSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:cd05945    82 EILDAAKPALLIAD--------GDD---------------------------NAYIIFTSGSTGRPKGVQISHDNLVSFT 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALV--P 739
Cdd:cd05945   127 NWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYPALASGATLVPVPRDATADPKQLFRFLAEHGITVWVSTPSFAAMCLlsP 206

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  740 ELSGPALAGIR-VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTS-GPTSEDSIRRGS--IGVPIDNTQVYVL 813
Cdd:cd05945   207 TFTPESLPSLRhFLFCGEVLPHKTARALQQRfpDARIYNTYGPTEATVAVTYiEVTPEVLDGYDRlpIGYAKPGAKLVIL 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  814 DANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFR 893
Cdd:cd05945   287 DEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPD----EGQRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYR 362

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  894 IELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVA---PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNG 970
Cdd:cd05945   363 IELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEaglTKAIKAELAERLPPYMIPRRFVYLDELPLNANG 442


                  ....*..
gi 653678460  971 KLDRAAL 977
Cdd:cd05945   443 KIDRKAL 449
LCL_NRPS-like cd19540
LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; ...
 1082-1499 2.25e-113

LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380463 [Multi-domain]  Cd Length: 433  Bit Score: 368.29  E-value: 2.25e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTrIHPGPDGMPVQ-VADPAGGGAA 1160
Cdd:cd19540     1 RIPLSFAQQRLWFLNRLDGPSAAYNIPLALRLTGALDVDALRAALADVVARHESLRT-VFPEDDGGPYQvVLPAAEARPD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1161 LTERDAAPGtDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREP 1240
Cdd:cd19540    80 LTVVDVTED-ELAARLAEAARRGFDLTAELPLRARLFRLGPDEHVLVLVVHHIAADGWSMAPLARDLATAYAARRAGRAP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1241 ELDEPALAQADYAVWQR-----QSAQRGRYAAELDFWRTELTGAVA-TEVAGDLPRPATPGGGGGAVEFALAPRQIQGIR 1314
Cdd:cd19540   159 DWAPLPVQYADYALWQRellgdEDDPDSLAARQLAYWRETLAGLPEeLELPTDRPRPAVASYRGGTVEFTIDAELHARLA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1315 ELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTA 1394
Cdd:cd19540   239 ALAREHGATLFMVLHAALAVLLSRLGAGDDIPIGTPVAGRGDEALDDLVGMFVNTLVLRTDVSGDPTFAELLARVRETDL 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1395 DALAHQELPFEHLVA----DRGAGNGPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVD------GGST 1464
Cdd:cd19540   319 AAFAHQDVPFERLVEalnpPRSTARHPLFQVMLAFQNTAAATLELPGLTVEPVPVDTGVAKFDLSFTLTErrdadgAPAG 398

                         410       420       430
                  ....*....|....*....|....*....|....*
gi 653678460 1465 LEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19540   399 LTGELEYATDLFDRSTAERLADRFVRVLEAVVADP 433
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
 503-977 6.10e-113

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 367.02  E-value: 6.10e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  503 RTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAY 582
Cdd:cd17653     9 AHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQA 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  583 LLSDARPVLVVTDAAtadrlgPDDitglvvleetdtggypateppavpaghSAYVIYTSGSTGRPKGVVITRAALDNFLA 662
Cdd:cd17653    89 ILRTSGATLLLTTDS------PDD---------------------------LAYIIFTSGSTGIPKGVMVPHRGVLNYVS 135

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  663 AMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADAdtarnPAALIDLAgrHRVTLAQATPTLWQALVPElS 742
Cdd:cd17653   136 QPPARLDVGPGSRVAQVLSIAFDACIGEIFSTLCNGGTLVLADP-----SDPFAHVA--RTVDALMSTPSILSTLSPQ-D 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  743 GPALAGIrvLVGGEALPAELARRLGdAGAEVTNMYGPTETTIWSTSGPTSEDsiRRGSIGVPIDNTQVYVLDANLHPAPI 822
Cdd:cd17653   208 FPNLKTI--FLGGEAVPPSLLDRWS-PGRRLYNAYGPTECTISSTMTELLPG--QPVTIGKPIPNSTCYILDADLQPVPE 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  823 GVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETT 902
Cdd:cd17653   283 GVVGEICISGVQVARGYLGNPALTASKFVPDPFW-PGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEV 361

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  903 LI-NAGPVRRAAAVVREDrpgdlRLVAYVIPDGpVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd17653   362 VLqSQPEVTQAAAIVVNG-----RLVAFVTPET-VDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKAL 431
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
 493-979 8.12e-113

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 367.21  E-value: 8.12e-113
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  493 LRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:COG0318     1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFPVERIAYLLSDARPVLVVTdaatadrlgpdditglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVI 652
Cdd:COG0318    81 PRLTAEELAYILEDSGARALVT----------------------------------------ALILYTSGTTGRPKGVML 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  653 TRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLADAdtaRNPAALIDLAGRHRVTLAQATP 731
Cdd:COG0318   121 THRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGlTVGLLAPLLAGATLVLLPR---FDPERVLELIERERVTVLFGVP 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQALV--PELSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDN 807
Cdd:COG0318   198 TMLARLLrhPEFARYDLSSLRLVVsGGAPLPPELLERFEERfGVRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPG 277

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  808 TQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQV 887
Cdd:COG0318   278 VEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGW-------LRTGDLGRLDEDGYLYIVGRKKDMI 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  888 KLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:COG0318   350 ISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVlrPGAELDAEELRAFLRERLARYKVPRRVEFVDELP 429

                         490
                  ....*....|....
gi 653678460  966 TTPNGKLDRAALPA 979
Cdd:COG0318   430 RTASGKIDRRALRE 443
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
 1535-2033 1.46e-107

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 351.61  E-value: 1.46e-107
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1535 QLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHAL-HTGalgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:cd17653     1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLlQLG------VVPGDVVPLLSDRSLEMLVAILAILKAGAAY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPMARLTRIADTVTPALVLTRTGqggclpgaevfgdvpvvaldrvadrltampdqrpevtvdPRGLAYSIFTSG 1693
Cdd:cd17653    75 VPLDAKLPSARIQAILRTSGATLLLTTDS---------------------------------------PDDLAYIIFTSG 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1694 STGQPKGVAVEHIGIVRYLSWAAAS-YPTAGRRgTLAHSSVGFDLTMSALFEPLvsGRGVTLMPAD--ATLADLAEELsg 1770
Cdd:cd17653   116 STGIPKGVMVPHRGVLNYVSQPPARlDVGPGSR-VAQVLSIAFDACIGEIFSTL--CNGGTLVLADpsDPFAHVARTV-- 190

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1771 playDYIRLTPSHLrhlvghwtgQELPPAA----RGWVVGGETLDPALVKqllELRPDAEVINHYGPTETVIGrVVHPvr 1846
Cdd:cd17653   191 ----DALMSTPSIL---------STLSPQDfpnlKTIFLGGEAVPPSLLD---RWSPGRRLYNAYGPTECTIS-STMT-- 251

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1847 eagELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAgEPGARMYRTGDL 1926
Cdd:cd17653   252 ---ELLPGQPVTIGKPIPNSTCYILDADLQPVPEGVVGEICISGVQVARGYLGNPALTASKFVPDPF-WPGSRMYRTGDY 327

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1927 VRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARM-AEHPGVEQAVVLVRDQQLVGWFIPAEdhppVTVSALRRFCAEQL 2005
Cdd:cd17653   328 GRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVlQSQPEVTQAAAIVVNGRLVAFVTPET----VDVDGLRSELAKHL 403

                         490       500
                  ....*....|....*....|....*...
gi 653678460 2006 PEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17653   404 PSYAVPDRIIALDSFPLTANGKVDRKAL 431
AMP-binding pfam00501
AMP-binding enzyme;
497-890 7.16e-107

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 348.92  E-value: 7.16e-107
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   497 FQQWCRRTPGAVAVIEGD-TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:pfam00501    1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   576 PVERIAYLLSDARPVLVVTDAAT------ADRLGPDDITGLVVLEETDTGGY------------PATEPPAVPAGHSAYV 637
Cdd:pfam00501   81 PAEELAYILEDSGAKVLITDDALkleellEALGKLEVVKLVLVLDRDPVLKEeplpeeakpadvPPPPPPPPDPDDLAYI 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   638 IYTSGSTGRPKGVVITRAALDNFLAAMA----ERFPLTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLADADTARNP 712
Cdd:pfam00501  161 IYTSGTTGKPKGVMLTHRNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGlSLGLLGPLLAGATVVLPPGFPALDP 240

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   713 AALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTS 788
Cdd:pfam00501  241 AALLELIERYKVTVLYGVPTLLNMLLeaGAPKRALLSSLRlVLSGGAPLPPELARRFRELfGGALVNGYGLTETTGVVTT 320

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   789 GPTSEDSIRR-GSIGVPIDNTQVYVLDAN-LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtRMYRTG 866
Cdd:pfam00501  321 PLPLDEDLRSlGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDED-------GWYRTG 393

                          410       420
                   ....*....|....*....|....
gi 653678460   867 DLVRWSAAGDLEYLGRTDHQVKLR 890
Cdd:pfam00501  394 DLGRRDEDGYLEIVGRKKDQIKLG 417
Condensation pfam00668
Condensation domain; This domain is found in many multi-domain enzymes which synthesize ...
 47-473 9.04e-106

Condensation domain; This domain is found in many multi-domain enzymes which synthesize peptide antibiotics. This domain catalyzes a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pfam00550). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site.


Pssm-ID: 395541 [Multi-domain]  Cd Length: 454  Bit Score: 347.01  E-value: 9.04e-106
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460    47 VPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPV 126
Cdd:pfam00668    5 YPLSPAQKRMWFLEKLEPHSSAYNMPAVLKLTGELDPERLEKALQELINRHDALRTVFIRQENGEPVQVILEERPFELEI 84

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   127 IDV-------TEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG---- 195
Cdd:pfam00668   85 IDIsdlseseEEEAIEAFIQRDLQSPFDLEKGPLFRAGLFRIAENRHHLLLSMHHIIVDGVSLGILLRDLADLYQQllkg 164

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   196 -ELPAGvAAPAFRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRL 273
Cdd:pfam00668  165 ePLPLP-PKTPYKDYAEWLQQYLQSEDYQKDAAYWLEQLEGeLPVLQLPKDYARPADRSFKGDRLSFTLDEDTEELLRKL 243

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   274 AAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRND 353
Cdd:pfam00668  244 AKAHGTTLNDVLLAAYGLLLSRYTGQDDIVVGTPGSGRPSPDIERMVGMFVNTLPLRIDPKGGKTFSELIKRVQEDLLSA 323

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   354 FAHPQIPFDRLVRELRRDRIAGSQLLVQAM------AGTESTAVALTFGEAAGTEQPVDVALAKCDLDLSVLDDGDRLAV 427
Cdd:pfam00668  324 EPHQGYPFGDLVNDLRLPRDLSRHPLFDPMfsfqnyLGQDSQEEEFQLSELDLSVSSVIEEEAKYDLSLTASERGGGLTI 403

                          410       420       430       440
                   ....*....|....*....|....*....|....*....|....*.
gi 653678460   428 TLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEVDLLDTDE 473
Cdd:pfam00668  404 KIDYNTSLFDEETIERFAEHFKELLEQAIAHPSQPLSELDLLSDAE 449
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
 1541-2033 5.55e-103

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 338.84  E-value: 5.55e-103
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPpvgpeTPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY 1620
Cdd:cd05945     1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAG-----DPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASS 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 PMARLTRIADTVTPALVLtrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtVDPRGLAYSIFTSGSTGQPKG 1700
Cdd:cd05945    76 PAERIREILDAAKPALLI-----------------------------------------ADGDDNAYIIFTSGSTGRPKG 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1701 VAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADAT--LADLAEELSgPLAYDYIR 1778
Cdd:cd05945   115 VQISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYPALASGATLVPVPRDATadPKQLFRFLA-EHGITVWV 193

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1779 LTPSHLRHLVGHWT-GQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAgELAVDSPL 1857
Cdd:cd05945   194 STPSFAAMCLLSPTfTPESLPSLRHFLFCGEVLPHKTARALQQRFPDARIYNTYGPTEATVAVTYIEVTPE-VLDGYDRL 272

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1858 PLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPagepGARMYRTGDLVRWRGDGLLDF 1937
Cdd:cd05945   273 PIGYAKPGAKLVILDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDE----GQRAYRTGDLVRLEADGLLFY 348

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1938 VGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-----DQQLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPN 2012
Cdd:cd05945   349 RGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKykgekVTELIAFVVPKPGAEAGLTKAIKAELAERLPPYMIPR 428

                         490       500
                  ....*....|....*....|.
gi 653678460 2013 QWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05945   429 RFVYLDELPLNANGKIDRKAL 449
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
 1533-2033 4.86e-102

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 337.21  E-value: 4.86e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGGY 1612
Cdd:cd05918     1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRS--LG---VGPGVFVPLCFEKSKWAVVAMLAVLKAGGA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1613 FIPVDPGYPMARLTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtVDPRGLAYSIFTS 1692
Cdd:cd05918    76 FVPLDPSHPLQRLQEILQDTGAKVVLT----------------------------------------SSPSDAAYVIFTS 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1693 GSTGQPKGVAVEHIGIV-RYLSWAAASYPTAGRRgTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADLAEELSGp 1771
Cdd:cd05918   116 GSTGKPKGVVIEHRALStSALAHGRALGLTSESR-VLQFASYTFDVSILEIFTTLAAGGCLCIPSEEDRLNDLAGFINR- 193

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1772 LAYDYIRLTPSHLRHLvghwtGQELPPAARGWVVGGETLDPALVKQLLelrPDAEVINHYGPTETVIGRVVHPVREAGEl 1851
Cdd:cd05918   194 LRVTWAFLTPSVARLL-----DPEDVPSLRTLVLGGEALTQSDVDTWA---DRVRLINAYGPAECTIAATVSPVVPSTD- 264

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1852 avdsPLPLGRPLGeTRLQVLDA----WLeaVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPA------GEPGARMY 1921
Cdd:cd05918   265 ----PRNIGRPLG-ATCWVVDPdnhdRL--VPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPAwlkqegSGRGRRLY 337

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1922 RTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEH-PGVEQAVVLV-------RDQQLVGWFIPAEDHP--- 1990
Cdd:cd05918   338 RTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSlPGAKEVVVEVvkpkdgsSSPQLVAFVVLDGSSSgsg 417

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1991 -------------PVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05918   418 dgdslflepsdefRALVAELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRAL 473
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
 1545-2033 1.00e-100

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 332.12  E-value: 1.00e-100
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd17650     1 PDAIAVSDATRQLTYRELNERANQLARTLR--GLG---VAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAER 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd17650    76 LQYMLEDSGAKLLLT-----------------------------------------QPEDLAYVIYTSGTTGKPKGVMVE 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVR-YLSW----AAASYPTAgrrgTLAHSSVGFDLTMSALFEPLVSGRGVTLMPaDATLADLAEelsgplAYDYIR- 1778
Cdd:cd17650   115 HRNVAHaAHAWrreyELDSFPVR----LLQMASFSFDVFAGDFARSLLNGGTLVICP-DEVKLDPAA------LYDLILk 183

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1779 -------LTPSHLRHLVGHWTGQEL-PPAARGWVVGGETLDPALVKQLL-ELRPDAEVINHYGPTETVIGRVVHPVREAG 1849
Cdd:cd17650   184 sritlmeSTPALIRPVMAYVYRNGLdLSAMRLLIVGSDGCKAQDFKTLAaRFGQGMRIINSYGVTEATIDSTYYEEGRDP 263

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 ELAVDSpLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGePGARMYRTGDLVRW 1929
Cdd:cd17650   264 LGDSAN-VPIGRPLPNTAMYVLDERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFA-PGERMYRTGDLARW 341

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-----QQLVGWFIPAEdhpPVTVSALRRFCAEQ 2004
Cdd:cd17650   342 RADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREdkggeARLCAYVVAAA---TLNTAELRAFLAKE 418

                         490       500
                  ....*....|....*....|....*....
gi 653678460 2005 LPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17650   419 LPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
 1545-2033 6.57e-98

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 325.38  E-value: 6.57e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd12114     1 PDATAVICGDGTLTYGELAERARRVAGALK--AAG---VRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAAR 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggCLPGAevFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd12114    76 REAILADAGARLVLT------DGPDA--QLDVAVFDVLILDLDALAAPAPPPPVDVAPDDLAYVIFTSGSTGTPKGVMIS 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPAD-----ATLADLAEEL------SGP-- 1771
Cdd:cd12114   148 HRAALNTILDINRRFAVGPDDRVLALSSLSFDLSVYDIFGALSAGATLVLPDEArrrdpAHWAELIERHgvtlwnSVPal 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1772 --LAYDYIRLTPSHLRHL-----VGHWTGQELPPAARgwvvggetldpalvkqllELRPDAEVINHYGPTETVIGRVVHP 1844
Cdd:cd12114   228 leMLLDVLEAAQALLPSLrlvllSGDWIPLDLPARLR------------------ALAPDARLISLGGATEASIWSIYHP 289

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1845 VREAgeLAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEpgaRMYRTG 1924
Cdd:cd12114   290 IDEV--PPDWRSIPYGRPLANQRYRVLDPRGRDCPDWVPGELWIGGRGVALGYLGDPELTAARFVTHPDGE---RLYRTG 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1925 DLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQ----QLVGWFIPAEDHPPVTVSALRRF 2000
Cdd:cd12114   365 DLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLGDpggkRLAAFVVPDNDGTPIAPDALRAF 444

                         490       500       510
                  ....*....|....*....|....*....|...
gi 653678460 2001 CAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd12114   445 LAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
 1534-2034 1.16e-96

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 320.27  E-value: 1.16e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1534 HQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGAlgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:cd17645     1 HQLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKG-----VKPDDQVGIMLDKSLDMIAAILGVLKAGGAY 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPMARLTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSG 1693
Cdd:cd17645    76 VPIDPDYPGERIAYMLADSSAKILLT-----------------------------------------NPDDLAYVIYTSG 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1694 STGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADA-----TLADLAEE- 1767
Cdd:cd17645   115 STGLPKGVMIEHHNLVNLCEWHRPYFGVTPADKSLVYASFSFDASAWEIFPHLTAGAALHVVPSERrldldALNDYFNQe 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1768 ------LSGPLAYDYIRLTPSHLRHLVghwtgqelppaargwvVGGETLDPALVKQLlelrpdaEVINHYGPTETVIGRV 1841
Cdd:cd17645   195 gitisfLPTGAAEQFMQLDNQSLRVLL----------------TGGDKLKKIERKGY-------KLVNNYGPTENTVVAT 251

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1842 VHPVREAgelavDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAgEPGARMY 1921
Cdd:cd17645   252 SFEIDKP-----YANIPIGKPIDNTRVYILDEALQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPF-VPGERMY 325

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1922 RTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQ-----LVGWFIPAEDHPPvtvSA 1996
Cdd:cd17645   326 RTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDAdgrkyLVAYVTAPEEIPH---EE 402

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 653678460 1997 LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17645   403 LREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKALP 440
SgcC5_NRPS-like cd19539
SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- ...
 1084-1499 1.54e-95

SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- bond forming activity and similar C-domains of modular NRPSs; SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. This subfamily also includes similar C-domains of modular NRPSs such as Penicillium chrysogenum N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase PCBAB. Condensation (C) domains of NRPSs normally catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380462 [Multi-domain]  Cd Length: 427  Bit Score: 316.63  E-value: 1.54e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAGGgAALTE 1163
Cdd:cd19539     3 PLSFAQERLWFIDQGEDGGPAYNIPGAWRLTGPLDVEALREALRDVVARHEALRTLLVRDDGGVPRQEILPPGP-APLEV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1164 RDAAPGTDLAQLLL-----AEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHR 1238
Cdd:cd19539    82 RDLSDPDSDRERRLeellrERESRGFDLDEEPPIRAVLGRFDPDDHVLVLVAHHTAFDAWSLDVFARDLAALYAARRKGP 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1239 EPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELAR 1318
Cdd:cd19539   162 AAPLPELRQQYKEYAAWQREALAAPRAAELLDFWRRRLRGAEPTALPTDRPRPAGFPYPGADLRFELDAELVAALRELAK 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1319 RCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALA 1398
Cdd:cd19539   242 RARSSLFMVLLAAYCVLLRRYTGQTDIVVGTPVAGRNHPRFESTVGFFVNLLPLRVDVSDCATFRDLIARVRKALVDAQR 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1399 HQELPFEHLVA----DRGAGNGPLFRIMYSF-SSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTLEGVLEYAD 1473
Cdd:cd19539   322 HQELPFQQLVAelpvDRDAGRHPLVQIVFQVtNAPAGELELAGGLSYTEGSDIPDGAKFDLNLTVTEEGTGLRGSLGYAT 401

                         410       420
                  ....*....|....*....|....*.
gi 653678460 1474 DLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19539   402 SLFDEETIQGFLADYLQVLRQLLANP 427
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
 1544-2034 2.08e-93

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 312.49  E-value: 2.08e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1544 TPGWTALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMA 1623
Cdd:cd17656     1 TPDAVAVVFENQKLTYRELNERSNQLARFLRE--KG---VKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEE 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1624 RLTRIADTVTPALVLTRTGqggcLPGAEVFGDVPVVALDrvaDRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAV 1703
Cdd:cd17656    76 RRIYIMLDSGVRVVLTQRH----LKSKLSFNKSTILLED---PSISQEDTSNIDYINNSDDLLYIIYTSGTTGKPKGVQL 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1704 EHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLaDLaEELSGPLAYDYIRLT--P 1781
Cdd:cd17656   149 EHKNMVNLLHFEREKTNINFSDKVLQFATCSFDVCYQEIFSTLLSGGTLYIIREETKR-DV-EQLFDLVKRHNIEVVflP 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1782 SHLRHLVGHWTG--QELPPAARGWVVGGETLdpaLVKQLLE---LRPDAEVINHYGPTET--VIGRVVHPVREAGELAvd 1854
Cdd:cd17656   227 VAFLKFIFSEREfiNRFPTCVKHIITAGEQL---VITNEFKemlHEHNVHLHNHYGPSEThvVTTYTINPEAEIPELP-- 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1855 splPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAgEPGARMYRTGDLVRWRGDGL 1934
Cdd:cd17656   302 ---PIGKPISNTWIYILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPF-DPNERMYRTGDLARYLPDGN 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1935 LDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQ-----LVGWFIPAEDhppVTVSALRRFCAEQLPEFM 2009
Cdd:cd17656   378 IEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDkgekyLCAYFVMEQE---LNISQLREYLAKQLPEYM 454

                         490       500
                  ....*....|....*....|....*
gi 653678460 2010 VPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17656   455 IPSFFVPLDQLPLTPNGKVDRKALP 479
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
 1545-2034 5.80e-93

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 310.10  E-value: 5.80e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPpvgpETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:cd17648     1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAEIRP----DDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDER 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 LTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd17648    77 IQFILEDTGARVVIT-----------------------------------------NSTDLAYAIYTSGTTGKPKGVLVE 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 HIGIVRYLSWAAASYPTAGRR--GTLAHSSVGFDLTMSALFEPLVSGRGVTLMP-----ADATLADLAEE-----LSGPL 1772
Cdd:cd17648   116 HGSVVNLRTSLSERYFGRDNGdeAVLFFSNYVFDFFVEQMTLALLNGQKLVVPPdemrfDPDRFYAYINRekvtyLSGTP 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 A----YDYIRLTpsHLRHLVghwtgqelppaargwvVGGETLDPALVKQLLELRPdAEVINHYGPTETVIGRVVHPvrEA 1848
Cdd:cd17648   196 SvlqqYDLARLP--HLKRVD----------------AAGEEFTAPVFEKLRSRFA-GLIINAYGPTETTVTNHKRF--FP 254

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1849 GELAVDSPLplGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPG-------ARMY 1921
Cdd:cd17648   255 GDQRFDKSL--GRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQTEQerargrnARLY 332

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1922 RTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR----------DQQLVGWFIPAEDHpp 1991
Cdd:cd17648   333 KTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKedasqaqsriQKYLVGYYLPEPGH-- 410

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 653678460 1992 VTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd17648   411 VPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRALP 453
SgcC5_NRPS-like cd19539
SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- ...
 47-459 2.37e-88

SgcC5 is a non-ribosomal peptide synthetase (NRPS) condensation enzyme with ester- and amide- bond forming activity and similar C-domains of modular NRPSs; SgcC5 is a free-standing NRPS condensation enzyme (rather than a modular NRPS), which catalyzes the condensation between the SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and (R)-1phenyl-1,2-ethanediol, forming an ester bond, during the synthesis of the chromoprotein enediyne antitumor antibiotic C-1027. It has some acceptor substrate promiscuity as it has been shown to also catalyze the formation of an amide bond between SgcC2-tethered (S)-3-chloro-5-hydroxy-beta-tyrosine and a mimic of the enediyne core acceptor substrate having an amine at its C-2 position. This subfamily also includes similar C-domains of modular NRPSs such as Penicillium chrysogenum N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase PCBAB. Condensation (C) domains of NRPSs normally catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380462 [Multi-domain]  Cd Length: 427  Bit Score: 295.83  E-value: 2.37e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   47 VPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPV 126
Cdd:cd19539     2 IPLSFAQERLWFIDQGEDGGPAYNIPGAWRLTGPLDVEALREALRDVVARHEALRTLLVRDDGGVPRQEILPPGPAPLEV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  127 IDVTEADLPQA------LRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGeLPAG 200
Cdd:cd19539    82 RDLSDPDSDRErrleelLRERESRGFDLDEEPPIRAVLGRFDPDDHVLVLVAHHTAFDAWSLDVFARDLAALYAA-RRKG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  201 VAAP------AFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLA 274
Cdd:cd19539   161 PAAPlpelrqQYKEYAAWQREALAAPRAAELLDFWRRRLRGAEPTALPTDRPRPAGFPYPGADLRFELDAELVAALRELA 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  275 AAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDF 354
Cdd:cd19539   241 KRARSSLFMVLLAAYCVLLRRYTGQTDIVVGTPVAGRNHPRFESTVGFFVNLLPLRVDVSDCATFRDLIARVRKALVDAQ 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  355 AHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAvALTFGEAAGTEQPVDVAL---AKCDLDLSVLDDGDRLAVTLVA 431
Cdd:cd19539   321 RHQELPFQQLVAELPVDRDAGRHPLVQIVFQVTNAP-AGELELAGGLSYTEGSDIpdgAKFDLNLTVTEEGTGLRGSLGY 399

                         410       420
                  ....*....|....*....|....*...
gi 653678460  432 AADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19539   400 ATSLFDEETIQGFLADYLQVLRQLLANP 427
LCL_NRPS cd19538
LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; ...
 47-452 1.20e-87

LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380461 [Multi-domain]  Cd Length: 432  Bit Score: 293.79  E-value: 1.20e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   47 VPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFrlgP--DGEPVQQTAPAAPVAL 124
Cdd:cd19538     2 IPLSFAQRRLWFLHQLEGPSATYNIPLVIKLKGKLDVQALQQALYDVVERHESLRTVF---PeeDGVPYQLILEEDEATP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  125 P--VIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY----AGELP 198
Cdd:cd19538    79 KleIKEVDEEELESEINEAVRYPFDLSEEPPFRATLFELGENEHVLLLLLHHIAADGWSLAPLTRDLSKAYrarcKGEAP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  199 AGVAAPA-FRDVAAWQ-----HGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEAAVR 271
Cdd:cd19538   159 ELAPLPVqYADYALWQqellgDESDPDSLIARQLAYWKKQLAGLPDeIELPTDYPRPAESSYEGGTLTFEIDSELHQQLL 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  272 RLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSR 351
Cdd:cd19538   239 QLAKDNNVTLFMVLQAGFAALLTRLGAGTDIPIGSPVAGRNDDSLEDLVGFFVNTLVLRTDTSGNPSFRELLERVKETNL 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  352 NDFAHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTESTAVA-LTFGEAAGTEQPVDVALAKCDLDLSV---LDDG--DRL 425
Cdd:cd19538   319 EAYEHQDIPFERLVEALNPTRSRSRHPLFQIMLALQNTPQPsLDLPGLEAKLELRTVGSAKFDLTFELreqYNDGtpNGI 398

                         410       420
                  ....*....|....*....|....*..
gi 653678460  426 AVTLVAAADLFTPAAAEQLAGQFLSLL 452
Cdd:cd19538   399 EGFIEYRTDLFDHETIEALAQRYLLLL 425
C_PKS-NRPS cd19532
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 47-459 2.67e-87

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHxxxD motif, a few such as Monascus pilosus lovastatin nonaketide synthase MokA have a non-canonical HRxxxD motif in the C-domain and are unable to catalyze amide-bond formation. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380455 [Multi-domain]  Cd Length: 421  Bit Score: 292.44  E-value: 2.67e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   47 VPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGP-DGEPVQQTAPAAPVALP 125
Cdd:cd19532     2 EPMSFGQSRFWFLQQYLEDPTTFNVTFSYRLTGPLDVARLERAVRAVGQRHEALRTCFFTDPeDGEPMQGVLASSPLRLE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  126 VIDVT-EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGElPAGVAAP 204
Cdd:cd19532    82 HVQISdEAEVEEEFERLKNHVYDLESGETMRIVLLSLSPTEHYLIFGYHHIAMDGVSFQIFLRDLERAYNGQ-PLLPPPL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  205 AFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD----LEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLAAAHDTT 280
Cdd:cd19532   161 QYLDFAARQRQDYESGALDEDLAYWKSEFSTLPEplplLPFAKVKSRPPLTRYDTHTAERRLDAALAARIKEASRKLRVT 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  281 PFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDFAHPQIP 360
Cdd:cd19532   241 PFHFYLAALQVLLARLLDVDDICIGIADANRTDEDFMETIGFFLNLLPLRFRRDPSQTFADVLKETRDKAYAALAHSRVP 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  361 FDRLVRELRRDRIAGSQLLVQA-----MAGTESTavalTFGEAAGTEqpVDVALAK--CDLDLSVLDDGDRLA-VTLVAA 432
Cdd:cd19532   321 FDVLLDELGVPRSATHSPLFQVfinyrQGVAESR----PFGDCELEG--EEFEDARtpYDLSLDIIDNPDGDClLTLKVQ 394

                         410       420
                  ....*....|....*....|....*..
gi 653678460  433 ADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19532   395 SSLYSEEDAELLLDSYVNLLEAFARDP 421
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
 229-1039 8.44e-84

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 305.07  E-value: 8.44e-84
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   229 WRQRLAGLPDLEIPGDRQRPADRDWRAHSVRRELPAATeaavrrLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVpv 308
Cdd:TIGR03443    2 WSERLDNPTLSVLPHDYLRPANNRLVEATYSLQLPSAE------VTAGGGSTPFIILLAAFAALVYRLTGDEDIVLGT-- 73

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   309 agRGRTDLESVVglfagmvpVRLDLTGRPSFDEVLSRTVAGSRNDFAHPQIPFDRLVRElrrdriagsqllVQAMAGTES 388
Cdd:TIGR03443   74 --SSNKSGRPFV--------LRLNITPELSFLQLYAKVSEEEKEGASDIGVPFDELSEH------------IQAAKKLER 131

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   389 TAV--ALTFGEAAGTEQPVDVALAKCDLDLSVLDDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAPDRLVSEV 466
Cdd:TIGR03443  132 TPPlfRLAFQDAPDNQQTTYSTGSTTDLTVFLTPSSPELELSIYYNSLLFSSDRITIVADQLAQLLSAASSNPDEPIGKV 211

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   467 DLLdTDELHTVLcrwndtarPLPAASL---------RERFQQWCRRTPGAVAVIE---------GDTTLSYRELDERANR 528
Cdd:TIGR03443  212 SLI-TPSQKSLL--------PDPTKDLdwsgfrgaiHDIFADNAEKHPDRTCVVEtpsfldpssKTRSFTYKQINEASNI 282

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   529 LARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP--VLVVTDAATADRLGPDD 606
Cdd:TIGR03443  283 LAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQTIYLSVAKPraLIVIEKAGTLDQLVRDY 362

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   607 ITG----------LVVLEE-TDTGGYPATEPPAVPAGHSAY------VI----------YTSGSTGRPKGVVITRAALDN 659
Cdd:TIGR03443  363 IDKelelrteipaLALQDDgSLVGGSLEGGETDVLAPYQALkdtptgVVvgpdsnptlsFTSGSEGIPKGVLGRHFSLAY 442

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   660 FLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALvp 739
Cdd:TIGR03443  443 YFPWMAKRFGLSENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTADDIGTPGRLAEWMAKYGATVTHLTPAMGQLL-- 520

                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   740 elSGPALAGIRVL-----VGgEALPAELARRLgDAGAE---VTNMYGPTET-------TIWSTSGPTS-----EDSIrrg 799
Cdd:TIGR03443  521 --SAQATTPIPSLhhaffVG-DILTKRDCLRL-QTLAEnvcIVNMYGTTETqravsyfEIPSRSSDSTflknlKDVM--- 593

                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   800 SIGVPIDNTQVYVLDANLHPAPIGV--LGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGT----------------- 860
Cdd:TIGR03443  594 PAGKGMKNVQLLVVNRNDRTQTCGVgeVGEIYVRAGGLAEGYLGLPELNAEKFVNNWFVDPSHwidldkennkperefwl 673

                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   861 ----RMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP---- 932
Cdd:TIGR03443  674 gprdRLYRTGDLGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPLVRENVTLVRRDKDEEPTLVSYIVPqdks 753

                          810       820       830       840       850       860       870       880
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   933 ---------------DGPVAP---------DALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTA 988
Cdd:TIGR03443  754 deleefksevddeesSDPVVKglikyrkliKDIREYLKKKLPSYAIPTVIVPLKKLPLNPNGKVDKPALPFPDTAQLAAV 833

                          890       900       910       920       930       940
                   ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460   989 RPPRD---------DRERFLCTAFAEVLGLPEVAVS--DNFFELGGHSLLAFRLLARIRDEF 1039
Cdd:TIGR03443  834 AKNRSasaadeeftETEREIRDLWLELLPNRPATISpdDSFFDLGGHSILATRMIFELRKKL 895
LCL_NRPS cd19538
LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; ...
 1083-1499 3.89e-83

LCL-type Condensation domain of non-ribosomal peptide synthetases (NRPSs) and similar domains; LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.


Pssm-ID: 380461 [Multi-domain]  Cd Length: 432  Bit Score: 281.08  E-value: 3.89e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1083 LPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTrIHPGPDGMPVQ-VADPAGGGAAL 1161
Cdd:cd19538     2 IPLSFAQRRLWFLHQLEGPSATYNIPLVIKLKGKLDVQALQQALYDVVERHESLRT-VFPEEDGVPYQlILEEDEATPKL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPgTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREPE 1241
Cdd:cd19538    81 EIKEVDE-EELESEINEAVRYPFDLSEEPPFRATLFELGENEHVLLLLLHHIAADGWSLAPLTRDLSKAYRARCKGEAPE 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1242 LDEPALAQADYAVWQRQ-----SAQRGRYAAELDFWRTELTGA-VATEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRE 1315
Cdd:cd19538   160 LAPLPVQYADYALWQQEllgdeSDPDSLIARQLAYWKKQLAGLpDEIELPTDYPRPAESSYEGGTLTFEIDSELHQQLLQ 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1316 LARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTAD 1395
Cdd:cd19538   240 LAKDNNVTLFMVLQAGFAALLTRLGAGTDIPIGSPVAGRNDDSLEDLVGFFVNTLVLRTDTSGNPSFRELLERVKETNLE 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1396 ALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTV-----VDGGSTLE 1466
Cdd:cd19538   320 AYEHQDIPFERLVealnPTRSRSRHPLFQIMLALQNTPQPSLDLPGLEAKLELRTVGSAKFDLTFELreqynDGTPNGIE 399

                         410       420       430
                  ....*....|....*....|....*....|...
gi 653678460 1467 GVLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19538   400 GFIEYRTDLFDHETIEALAQRYLLLLESAVENP 432
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
495-977 2.26e-80

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 275.62  E-value: 2.26e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:PRK04813    6 ETIEEFAQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGHAYIPVDVS 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVV-TDAATADRLGPDDITgLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVIT 653
Cdd:PRK04813   86 SPAERIEMIIEVAKPSLIIaTEELPLEILGIPVIT-LDELKDIFATGNPYDFDHAVKGDDNYYIIFTSGTTGKPKGVQIS 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  654 RAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDlagrhrvTLAQ----- 728
Cdd:PRK04813  165 HDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYPTLASGGTLVALPKDMTANFKQLFE-------TLPQlpinv 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  729 --ATPTLWQA--LVPELSGPALAGIRV-LVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGS- 800
Cdd:PRK04813  238 wvSTPSFADMclLDPSFNEEHLPNLTHfLFCGEELPHKTAKKLLERfpSATIYNTYGPTEATVAVTSIEITDEMLDQYKr 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  801 --IGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpFGPPGTRMYRTGDLVRWsAAGDLE 878
Cdd:PRK04813  318 lpIGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAF----FTFDGQPAYHTGDAGYL-EDGLLF 392

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  879 YLGRTDHQVKLRGFRIELGEIETTLiNAGPVRRAAAVVREDRPGDLR-LVAYVIP-DGPVAPD-----ALRTELSRTLPD 951
Cdd:PRK04813  393 YQGRIDFQIKLNGYRIELEEIEQNL-RQSSYVESAVVVPYNKDHKVQyLIAYVVPkEEDFEREfeltkAIKKELKERLME 471

                         490       500
                  ....*....|....*....|....*.
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK04813  472 YMIPRKFIYRDSLPLTPNGKIDRKAL 497
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
 1533-2033 4.32e-80

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 272.84  E-value: 4.32e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGY 1612
Cdd:COG0318     1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALR--ALG---VGPGDRVALLLPNSPEFVVAFLAALRAGAV 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1613 FIPVDPGYPMARLTRIADTVTPALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvdprglAYSIFTS 1692
Cdd:COG0318    76 VVPLNPRLTAEELAYILEDSGARALVT----------------------------------------------ALILYTS 109

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1693 GSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSA-LFEPLVSGRGVTLMP---ADATLADLAEEl 1768
Cdd:COG0318   110 GTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVgLLAPLLAGATLVLLPrfdPERVLELIERE- 188

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1769 sGPlayDYIRLTPSHLRHLVGH--WTGQELPpAARGWVVGGETLDPALVKQLLElRPDAEVINHYGPTETVIGRVVHPvr 1846
Cdd:COG0318   189 -RV---TVLFGVPTMLARLLRHpeFARYDLS-SLRLVVSGGAPLPPELLERFEE-RFGVRIVEGYGLTETSPVVTVNP-- 260

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1847 eaGELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpagEPGarMYRTGDL 1926
Cdd:COG0318   261 --EDPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF------RDG--WLRTGDL 330

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1927 VRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-----VRDQQLVGWFIPAEDHpPVTVSALRRFC 2001
Cdd:COG0318   331 GRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVgvpdeKWGERVVAFVVLRPGA-ELDAEELRAFL 409

                         490       500       510
                  ....*....|....*....|....*....|..
gi 653678460 2002 AEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:COG0318   410 RERLARYKVPRRVEFVDELPRTASGKIDRRAL 441
D-ala-DACP-lig TIGR01734
D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also ...
 492-977 3.93e-79

D-alanine--poly(phosphoribitol) ligase, subunit 1; This model represents the enzyme (also called D-alanine-D-alanyl carrier protein ligase) which activates D-alanine as an adenylate via the reaction D-ala + ATP -> D-ala-AMP + PPi, and further catalyzes the condensation of the amino acid adenylate with the D-alanyl carrier protein (D-ala-ACP). The D-alanine is then further transferred to teichoic acid in the biosynthesis of lipoteichoic acid (LTA) and wall teichoic acid (WTA) in gram positive bacteria, both polysacchatides. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273780 [Multi-domain]  Cd Length: 502  Bit Score: 272.02  E-value: 3.93e-79
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   492 SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPI 571
Cdd:TIGR01734    1 KLIEAIQAFAETYPQTIAYRYQGQELTYQQLKEQSDRLAAFIQKRILPKKSPIIVYGHMEPHMLVAFLGSIKSGHAYIPV 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   572 DPDFPVERIAYLLSDARPVLVVTDAA-TADRLGPDDITgLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGV 650
Cdd:TIGR01734   81 DTSIPSERIEMIIEAAGPELVIHTAElSIDAVGTQIIT-LSALEQAETSGGPVSFDHAVKGDDNYYIIYTSGSTGNPKGV 159

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   651 VITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQAT 730
Cdd:TIGR01734  160 QISHDNLVSFTNWMLADFPLSEGKQFLNQAPFSFDLSVMDLYPCLASGGTLHCLDKDITNNFKLLFEELPKTGLNVWVST 239

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   731 PTLWQA--LVPELSGPALAGI-RVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTS-GPTSE--DSIRRGSIG 802
Cdd:TIGR01734  240 PSFVDMclLDPNFNQENYPHLtHFLFCGEELPVKTAKALLERfpKATIYNTYGPTEATVAVTSvKITQEilDQYPRLPIG 319

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   803 VPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpFGPPGTRMYRTGDLVRWSaAGDLEYLGR 882
Cdd:TIGR01734  320 FAKPDMNLFIMDEEGEPLPEGEKGEIVIVGPSVSKGYLNNPEKTAEAF----FSHEGQPAYRTGDAGTIT-DGQLFYQGR 394

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   883 TDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDL-RLVAYVIPDGPV------APDALRTELSRTLPDYMIP 955
Cdd:TIGR01734  395 LDFQIKLHGYRIELEDIEFNLRQSSYIESAVVVPKYNKDHKVeYLIAAIVPETEDfekefqLTKAIKKELKKSLPAYMIP 474

                          490       500
                   ....*....|....*....|..
gi 653678460   956 AVIVPVPDFPTTPNGKLDRAAL 977
Cdd:TIGR01734  475 RKFIYRDQLPLTANGKIDRKAL 496
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
 1271-2125 8.00e-77

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 283.11  E-value: 8.00e-77
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1271 FWRTELTGAVATEVAGDLPRPATpgggGGAVEfALAPRQIQGiRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGST 1350
Cdd:TIGR03443    1 RWSERLDNPTLSVLPHDYLRPAN----NRLVE-ATYSLQLPS-AEVTAGGGSTPFIILLAAFAALVYRLTGDEDIVLGTS 74

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1351 VSGRGNPrldnlvgclvntVVLRGDLSGAPTFHELLRRTHARTADALAHQELPFEHLVADRGAGNG-----PLFRImySF 1425
Cdd:TIGR03443   75 SNKSGRP------------FVLRLNITPELSFLQLYAKVSEEEKEGASDIGVPFDELSEHIQAAKKlertpPLFRL--AF 140

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1426 SSQEpagrsagDVDLEPVPVPVTTckfDLVLTVVDGGSTLEGVLEYADDLYlpgTAER---LVTSLTNLLAAAVRTPELA 1502
Cdd:TIGR03443  141 QDAP-------DNQQTTYSTGSTT---DLTVFLTPSSPELELSIYYNSLLF---SSDRitiVADQLAQLLSAASSNPDEP 207

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1503 VTRLAITSES------DTVRTSRWGrseQHVGddrTVHQLVEAQA----DR-----TPGWTALRTGDRSLTFAGLDAQAN 1567
Cdd:TIGR03443  208 IGKVSLITPSqksllpDPTKDLDWS---GFRG---AIHDIFADNAekhpDRtcvveTPSFLDPSSKTRSFTYKQINEASN 281

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1568 RLAHAL-HTGalgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLT------------------RI 1628
Cdd:TIGR03443  282 ILAHYLlKTG------IKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQTiylsvakpraliviekagTL 355

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1629 ADTVT-------------PALVLTRTG--QGGCLPGAEvfGDV--PVVALDrvadrltampDQRPEVTVDPRGLAYSIFT 1691
Cdd:TIGR03443  356 DQLVRdyidkelelrteiPALALQDDGslVGGSLEGGE--TDVlaPYQALK----------DTPTGVVVGPDSNPTLSFT 423

                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1692 SGSTGQPKGVAVEHIGIVRYLSWAAASYP-TAGRRGTL----AHSSVGFDLtmsalFEPLVSGRGVTLMPAD--ATLADL 1764
Cdd:TIGR03443  424 SGSEGIPKGVLGRHFSLAYYFPWMAKRFGlSENDKFTMlsgiAHDPIQRDM-----FTPLFLGAQLLVPTADdiGTPGRL 498

                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1765 AEELSgplayDY----IRLTPSHLRHLVGHWTGQeLPPAARGWVVGGetldpALVKQ----LLELRPDAEVINHYGPTET 1836
Cdd:TIGR03443  499 AEWMA-----KYgatvTHLTPAMGQLLSAQATTP-IPSLHHAFFVGD-----ILTKRdclrLQTLAENVCIVNMYGTTET 567

                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1837 viGRVVH----PVREAGELAVDSP---LPLGRPLGETRLQVLDAWLEAVP--VGAVGELFIGGDGIARGYLGRPALTAEK 1907
Cdd:TIGR03443  568 --QRAVSyfeiPSRSSDSTFLKNLkdvMPAGKGMKNVQLLVVNRNDRTQTcgVGEVGEIYVRAGGLAEGYLGLPELNAEK 645

                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1908 FLP------------DPAGEPGA---------RMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPG 1966
Cdd:TIGR03443  646 FVNnwfvdpshwidlDKENNKPErefwlgprdRLYRTGDLGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPL 725

                          810       820       830       840       850       860       870       880
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1967 VEQAVVLVR-----DQQLVGWFIP----------------AEDHPPVtVSALRR----------FCAEQLPEFMVPNQWV 2015
Cdd:TIGR03443  726 VRENVTLVRrdkdeEPTLVSYIVPqdksdeleefksevddEESSDPV-VKGLIKyrklikdireYLKKKLPSYAIPTVIV 804

                          890       900       910       920       930       940       950       960
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2016 ALDAFPLTPHGKVNHRALPGPTSGRPELEDRYQAPR------TPGEQLLAELWSTVLGT--DRIGIEDNFFDLGGTSISV 2087
Cdd:TIGR03443  805 PLKKLPLNPNGKVDKPALPFPDTAQLAAVAKNRSASaadeefTETEREIRDLWLELLPNrpATISPDDSFFDLGGHSILA 884

                          970       980       990
                   ....*....|....*....|....*....|....*....
gi 653678460  2088 IQLSGACRRA-GVEISPKDVFEQPTVRGQAmRAIARSRD 2125
Cdd:TIGR03443  885 TRMIFELRKKlNVELPLGLIFKSPTIKGFA-KEVDRLKK 922
AMP-binding pfam00501
AMP-binding enzyme;
1537-1948 1.34e-76

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 261.48  E-value: 1.34e-76
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1537 VEAQADRTPGWTALRTGD-RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIP 1615
Cdd:pfam00501    1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLR--ALG---VGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1616 VDPGYPMARLTRIADTVTPALVLTrTGQGGCLPGAEVFGDVPVVALDRVADRLTAM-------------PDQRPEVTVDP 1682
Cdd:pfam00501   76 LNPRLPAEELAYILEDSGAKVLIT-DDALKLEELLEALGKLEVVKLVLVLDRDPVLkeeplpeeakpadVPPPPPPPPDP 154

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1683 RGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRG----TLAHSSVGFDLTMSA-LFEPLVSGRGVTLMPA 1757
Cdd:pfam00501  155 DDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIKRVRPRGFGLGpddrVLSTLPLFHDFGLSLgLLGPLLAGATVVLPPG 234

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1758 DATLADLAeelsgplAYDYIR--------LTPSHLRHLVGHWTG-QELPPAARGWVVGGETLDPALVKQLLELRPDAeVI 1828
Cdd:pfam00501  235 FPALDPAA-------LLELIErykvtvlyGVPTLLNMLLEAGAPkRALLSSLRLVLSGGAPLPPELARRFRELFGGA-LV 306

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1829 NHYGPTETVIGrVVHPVREAGELAvdSPLPLGRPLGETRLQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEK 1907
Cdd:pfam00501  307 NGYGLTETTGV-VTTPLPLDEDLR--SLGSVGRPLPGTEVKIVDdETGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEA 383

                          410       420       430       440
                   ....*....|....*....|....*....|....*....|.
gi 653678460  1908 FLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLR 1948
Cdd:pfam00501  384 FDED-------GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
C_NRPS-like cd19066
Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of ...
 46-459 5.62e-75

Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long, with various activities such as antibiotic, antifungal, antitumor and immunosuppression. There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380453 [Multi-domain]  Cd Length: 427  Bit Score: 257.34  E-value: 5.62e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFrLGPDGEPVQQTAPAAPVALP 125
Cdd:cd19066     1 KIPLSPMQRGMWFLKKLATDPSAFNVAIEMFLTGSLDLARLKQALDAVMERHDVLRTRF-CEEAGRYEQVVLDKTVRFRI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  126 -VIDVTEADLPQA----LRTAAEQ-PFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG-ELP 198
Cdd:cd19066    80 eIIDLRNLADPEArlleLIDQIQQtIYDLERGPLVRVALFRLADERDVLVVAIHHIIVDGGSFQILFEDISSVYDAaERQ 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  199 AGVAAP---AFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLA 274
Cdd:cd19066   160 KPTLPPpvgSYADYAAWLEKQLESEAAQADLAYWTSYLHGLPPpLPLPKAKRPSQVASYEVLTLEFFLRSEETKRLREVA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  275 AAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDF 354
Cdd:cd19066   240 RESGTTPTQLLLAAFALALKRLTASIDVVIGLTFLNRPDEAVEDTIGLFLNLLPLRIDTSPDATFPELLKRTKEQSREAI 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  355 AHPQIPFDRLVRELRRDRIAGSQLLVQAMAGTEST-AVALTFGEAAGTEQPVDVAL-AKCDLDLSVLDDGDR-LAVTLVA 431
Cdd:cd19066   320 EHQRVPFIELVRHLGVVPEAPKHPLFEPVFTFKNNqQQLGKTGGFIFTTPVYTSSEgTVFDLDLEASEDPDGdLLLRLEY 399

                         410       420
                  ....*....|....*....|....*...
gi 653678460  432 AADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19066   400 SRGVYDERTIDRFAERYMTALRQLIENP 427
Condensation pfam00668
Condensation domain; This domain is found in many multi-domain enzymes which synthesize ...
 1084-1514 9.13e-75

Condensation domain; This domain is found in many multi-domain enzymes which synthesize peptide antibiotics. This domain catalyzes a condensation reaction to form peptide bonds in non- ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pfam00550). It has been shown that mutations in the HHXXXDG motif abolish activity suggesting this is part of the active site.


Pssm-ID: 395541 [Multi-domain]  Cd Length: 454  Bit Score: 257.65  E-value: 9.13e-75
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1084 PLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPA-------- 1155
Cdd:pfam00668    6 PLSPAQKRMWFLEKLEPHSSAYNMPAVLKLTGELDPERLEKALQELINRHDALRTVFIRQENGEPVQVILEErpfeleii 85

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1156 --GGGAALTERDAAPgtdlaQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAA 1233
Cdd:pfam00668   86 diSDLSESEEEEAIE-----AFIQRDLQSPFDLEKGPLFRAGLFRIAENRHHLLLSMHHIIVDGVSLGILLRDLADLYQQ 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1234 DTghREPELDEPALAQ-ADYAVWQRQSAQRGRYAAELDFWRTELTGAVA-TEVAGDLPRPATPGGGGGAVEFALAPRQIQ 1311
Cdd:pfam00668  161 LL--KGEPLPLPPKTPyKDYAEWLQQYLQSEDYQKDAAYWLEQLEGELPvLQLPKDYARPADRSFKGDRLSFTLDEDTEE 238

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1312 GIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHA 1391
Cdd:pfam00668  239 LLRKLAKAHGTTLNDVLLAAYGLLLSRYTGQDDIVVGTPGSGRPSPDIERMVGMFVNTLPLRIDPKGGKTFSELIKRVQE 318

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1392 RTADALAHQELPFEHLVAD----RGAGNGPLFRIMYSF-------SSQEPAGRSAGDVDLEPVPVPVTtcKFDLVLTVVD 1460
Cdd:pfam00668  319 DLLSAEPHQGYPFGDLVNDlrlpRDLSRHPLFDPMFSFqnylgqdSQEEEFQLSELDLSVSSVIEEEA--KYDLSLTASE 396

                          410       420       430       440       450
                   ....*....|....*....|....*....|....*....|....*....|....
gi 653678460  1461 GGSTLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDT 1514
Cdd:pfam00668  397 RGGGLTIKIDYNTSLFDEETIERFAEHFKELLEQAIAHPSQPLSELDLLSDAEK 450
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
1532-2033 1.54e-74

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 258.67  E-value: 1.54e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTpgwtALRTGDRSLTFAGLDAQANRLAHALHTGALGAppvgpETPVLLLLDRSPELVVAMLAVLKAGG 1611
Cdd:PRK04813    7 TIEEFAQTQPDFP----AYDYLGEKLTYGQLKEDSDALAAFIDSLKLPD-----KSPIIVFGHMSPEMLATFLGAVKAGH 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1612 YFIPVDPGYPMARLTRIADTVTPALVLTRTGqggcLPgaEVFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFT 1691
Cdd:PRK04813   78 AYIPVDVSSPAERIEMIIEVAKPSLIIATEE----LP--LEILGIPVITLDELKDIFATGNPYDFDHAVKGDDNYYIIFT 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1692 SGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADAT--LADLAEEL- 1768
Cdd:PRK04813  152 SGTTGKPKGVQISHDNLVSFTNWMLEDFALPEGPQFLNQAPYSFDLSVMDLYPTLASGGTLVALPKDMTanFKQLFETLp 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1769 --------SGPLAYDYIRLTPSHlrhlvghwtGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTE-TVIG 1839
Cdd:PRK04813  232 qlpinvwvSTPSFADMCLLDPSF---------NEEHLPNLTHFLFCGEELPHKTAKKLLERFPSATIYNTYGPTEaTVAV 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1840 RVVHPVREAgeLAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPdpagEPGAR 1919
Cdd:PRK04813  303 TSIEITDEM--LDQYKRLPIGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAFFT----FDGQP 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1920 MYRTGDLVRWRgDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-----QQLVGWFIPA----EDHP 1990
Cdd:PRK04813  377 AYHTGDAGYLE-DGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVVPYNkdhkvQYLIAYVVPKeedfEREF 455

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 653678460 1991 PVTvSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK04813  456 ELT-KAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
500-977 1.16e-71

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 251.95  E-value: 1.16e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  500 WCRRTPGAVAVI-EGDT----TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:COG0365    18 HAEGRGDKVALIwEGEDgeerTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPG 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDAATA----------------------------DRLGPD-DITGLVVLEETDTGGYPATE 625
Cdd:COG0365    98 FGAEALADRIEDAEAKVLITADGGLrggkvidlkekvdealeelpslehvivvGRTGADvPMEGDLDWDELLAAASAEFE 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  626 PPAVPAGHSAYVIYTSGSTGRPKGVVIT-RAALDNFLAAMAERFPLTAEDRVLATTTVSFdIAGL--ELYLPLRSGAGVV 702
Cdd:COG0365   178 PEPTDADDPLFILYTSGTTGKPKGVVHThGGYLVHAATTAKYVLDLKPGDVFWCTADIGW-ATGHsyIVYGPLLNGATVV 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  703 LAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALVPE----LSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTN 775
Cdd:COG0365   257 LYEgRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAgdepLKKYDLSSLRLLGsAGEPLNPEVWEWWYEAvGVPIVD 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  776 MYGPTETT-IWSTSGPTSEdsIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE--GLARGYWNRPGLTAEKFLP 852
Cdd:COG0365   337 GWGQTETGgIFISNLPGLP--VKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIKGPwpGMFRGYWNDPERYRETYFG 414

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  853 DPFGppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP 932
Cdd:COG0365   415 RFPG-----WYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVL 489

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 653678460  933 -DGPVAPDALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:COG0365   490 kPGVEPSDELAKELqahvREELGPYAYPREIEFVDELPKTRSGKIMRRLL 539
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
 501-979 1.35e-71

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 250.47  E-value: 1.35e-71
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   501 CRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERI 580
Cdd:TIGR03098   10 AARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQV 89

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   581 AYLLSDARPVLVVTDAATADRLGP-----DDITGLVV------------------LEETDTGGYPATEPPAVPAGHSAyV 637
Cdd:TIGR03098   90 AHILADCNVRLLVTSSERLDLLHPalpgcHDLRTLIIvgdpahaseghpgeepasWPKLLALGDADPPHPVIDSDMAA-I 168

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   638 IYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNpaaLID 717
Cdd:TIGR03098  169 LYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVVLHDYLLPRD---VLK 245

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   718 LAGRHRVTLAQATPTLWQALV----PELSGPALagiRVLVG-GEALPAELARRLGDA--GAEVTNMYGPTEtTIWSTSGP 790
Cdd:TIGR03098  246 ALEKHGITGLAAVPPLWAQLAqldwPESAAPSL---RYLTNsGGAMPRATLSRLRSFlpNARLFLMYGLTE-AFRSTYLP 321

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   791 TSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRT----G 866
Cdd:TIGR03098  322 PEEVDRRPDSIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAMGYWNDPEKTAERFRPLPPFPGELHLPELavwsG 401

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   867 DLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVIP--DGPVAPDALRT 943
Cdd:TIGR03098  402 DTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFgVPDPTLGQ-AIVLVVTPpgGEELDRAALLA 480

                          490       500       510
                   ....*....|....*....|....*....|....*.
gi 653678460   944 ELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPA 979
Cdd:TIGR03098  481 ECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
C_PKS-NRPS_PksJ-like cd20484
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 48-459 2.60e-71

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs), similar to Bacillus subtilis PksJ; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily have the typical C-domain HHxxxD motif. PksJ is involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is important in secondary metabolism. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380472 [Multi-domain]  Cd Length: 430  Bit Score: 246.84  E-value: 2.60e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQTAPAAPVALPVI 127
Cdd:cd20484     3 PLSEGQKGLWMLQKMSPEMSAYNVPLCFRFSSKLDVEKFKQACQFVLEQHPILKSVIE-EEDGVPFQKIEPSKPLSFQEE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  128 D---VTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA----GELPAG 200
Cdd:cd20484    82 DissLKESEIIAYLREKAKEPFVLENGPLMRVHLFSRSEQEHFVLITIHHIIFDGSSSLTLIHSLLDAYQallqGKQPTL 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  201 VAAPA-FRDVAAWQHGRLAAGELDDQLRYWRQRLAG-LPDLEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLAAAHD 278
Cdd:cd20484   162 ASSPAsYYDFVAWEQDMLAGAEGEEHRAYWKQQLSGtLPILELPADRPRSSAPSFEGQTYTRRLPSELSNQIKSFARSQS 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  279 TTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEV---LSRTVAgsrNDFA 355
Cdd:cd20484   242 INLSTVFLGIFKLLLHRYTGQEDIIVGMPTMGRPEERFDSLIGYFINMLPIRSRILGEETFSDFirkLQLTVL---DGLD 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  356 HPQIPFDRLVRELRRDR-IAGSQLL--------------VQAMAGTESTAVALTFGEAAGTEqpvdvalAKCDLDLSVLD 420
Cdd:cd20484   319 HAAYPFPAMVRDLNIPRsQANSPVFqvaffyqnflqstsLQQFLAEYQDVLSIEFVEGIHQE-------GEYELVLEVYE 391

                         410       420       430
                  ....*....|....*....|....*....|....*....
gi 653678460  421 DGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd20484   392 QEDRFTLNIKYNPDLFDASTIERMMEHYVKLAEELIANP 430
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
 633-973 8.94e-71

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 241.81  E-value: 8.94e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  633 HSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADtarNP 712
Cdd:cd04433     1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKF---DP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  713 AALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTS 788
Cdd:cd04433    78 EAALELIEREKVTILLGVPTLLARLLkaPESAGYDLSSLRALVsGGAPLPPELLERFEEApGIKLVNGYGLTETGGTVAT 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  789 GPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpfgppGTRMYRTGDL 868
Cdd:cd04433   158 GPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVD--------EDGWYRTGDL 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  869 VRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP--DGPVAPDALRTELS 946
Cdd:cd04433   230 GRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLrpGADLDAEELRAHVR 309

                         330       340
                  ....*....|....*....|....*..
gi 653678460  947 RTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd04433   310 ERLAPYKVPRRVVFVDALPRTASGKID 336
COG4908 COG4908
Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General ...
 49-282 4.85e-68

Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General function prediction only];


Pssm-ID: 443936 [Multi-domain]  Cd Length: 243  Bit Score: 229.93  E-value: 4.85e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   49 LAPGQAGIWFseqLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGpDGEPVQQTAPAAPVALPVID 128
Cdd:COG4908     1 LSPAQKRFLF---LEPGSNAYNIPAVLRLEGPLDVEALERALRELVRRHPALRTRFVEE-DGEPVQRIDPDADLPLEVVD 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  129 VT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG-----E 196
Cdd:COG4908    77 LSalpeperEAELEELVAEEASRPFDLARGPLLRAALIRLGEDEHVLLLTIHHIISDGWSLGILLRELAALYAAllegeP 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  197 LPAGVAAPAFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLAA 275
Cdd:COG4908   157 PPLPELPIQYADYAAWQRAWLQSEALEKQLEYWRQQLAGAPPvLELPTDRPRPAVQTFRGATLSFTLPAELTEALKALAK 236


                  ....*..
gi 653678460  276 AHDTTPF 282
Cdd:COG4908   237 AHGATVN 243
DCL_NRPS cd19543
DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the ...
 48-459 3.92e-67

DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor; The DCL-type Condensation (C) domain catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. This domain is D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains in addition to the LCL- and DCL-types such as starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380465 [Multi-domain]  Cd Length: 423  Bit Score: 234.40  E-value: 3.92e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPVI 127
Cdd:cd19543     3 PLSPMQEGMLFHSLLDPGSGAYVEQMVITLEGPLDPDRFRAAWQAVVDRHPILRTSFVWEGLGEPLQVVLKDRKLPWREL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  128 DVT-------EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA----GE 196
Cdd:cd19543    83 DLShlseaeqEAELEALAEEDRERGFDLARAPLMRLTLIRLGDDRYRLVWSFHHILLDGWSLPILLKELFAIYAalgeGQ 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  197 LPAGVAAPAFRDVAAWqhgrLAAGELDDQLRYWRQRLAGLPDL-EIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLAA 275
Cdd:cd19543   163 PPSLPPVRPYRDYIAW----LQRQDKEAAEAYWREYLAGFEEPtPLPKELPADADGSYEPGEVSFELSAELTARLQELAR 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  276 AHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR--GRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLsRTVAGSRND 353
Cdd:cd19543   239 QHGVTLNTVVQGAWALLLSRYSGRDDVVFGTTVSGRpaELPGIETMVGLFINTLPVRVRLDPDQTVLELL-KDLQAQQLE 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  354 F-AHPQIPfdrLVrELRRDRIAGSQLL----------VQAMAGTESTAVALTFGEAAGTEQPvdvalakcDLDLSVL-DD 421
Cdd:cd19543   318 LrEHEYVP---LY-EIQAWSEGKQALFdhllvfenypVDESLEEEQDEDGLRITDVSAEEQT--------NYPLTVVaIP 385

                         410       420       430
                  ....*....|....*....|....*....|....*...
gi 653678460  422 GDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19543   386 GEELTIKLSYDAEVFDEATIERLLGHLRRVLEQVAANP 423
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
 501-974 1.98e-65

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 229.80  E-value: 1.98e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  501 CRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERI 580
Cdd:cd17631     5 ARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEV 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  581 AYLLSDARPVLVVtdaatadrlgpDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVIT-RAALDN 659
Cdd:cd17631    85 AYILADSGAKVLF-----------DDL---------------------------ALLMYTSGTTGRPKGAMLThRNLLWN 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  660 FLAAMAErFPLTAEDRVLATTTVsFDIAGLELYLP---LRSGAGVVLADADtarnPAALIDLAGRHRVTLAQATPTLWQA 736
Cdd:cd17631   127 AVNALAA-LDLGPDDVLLVVAPL-FHIGGLGVFTLptlLRGGTVVILRKFD----PETVLDLIERHRVTSFFLVPTMIQA 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  737 LV--PELSGPALAGIR-VLVGGEALPAELARRLGDAGAEVTNMYGPTETTIwSTSGPTSEDSIRR-GSIGVPIDNTQVYV 812
Cdd:cd17631   201 LLqhPRFATTDLSSLRaVIYGGAPMPERLLRALQARGVKFVQGYGMTETSP-GVTFLSPEDHRRKlGSAGRPVFFVEVRI 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGF 892
Cdd:cd17631   280 VDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDGWF--------HTGDLGRLDEDGYLYIVDRKKDMIISGGE 351

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  893 RIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPN 969
Cdd:cd17631   352 NVYPAEVEDVLYEHPAVAEVAVIgVPDEKWGE-AVVAVVVprPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNAT 430


                  ....*
gi 653678460  970 GKLDR 974
Cdd:cd17631   431 GKILK 435
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
 515-980 2.94e-64

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 229.33  E-value: 2.94e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPvlvvt 594
Cdd:cd17647    19 RSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKP----- 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 daatadrlgpddiTGLVVLEETDTGGYPATEPPavpaghsayVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAED 674
Cdd:cd17647    94 -------------RGLIVIRAAGVVVGPDSNPT---------LSFTSGSEGIPKGVLGRHFSLAYYFPWMAKRFNLSEND 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  675 RVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPALAGIRVLVG 754
Cdd:cd17647   152 KFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTQDDIGTPGRLAEWMAKYGATVTHLTPAMGQLLTAQATTPFPKLHHAFFV 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  755 GEALPAELARRLgDAGAE---VTNMYGPTETT-------IWS-TSGPTSEDSIR------RGsigvpIDNTQVYVLDAN- 816
Cdd:cd17647   232 GDILTKRDCLRL-QTLAEnvrIVNMYGTTETQravsyfeVPSrSSDPTFLKNLKdvmpagRG-----MLNVQLLVVNRNd 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  817 -LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPG---------------------TRMYRTGDLVRWSAA 874
Cdd:cd17647   306 rTQICGIGEVGEIYVRAGGLAEGYRGLPELNKEKFVNNWFVEPDhwnyldkdnnepwrqfwlgprDRLYRTGDLGRYLPN 385

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  875 GDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDG-------------------- 934
Cdd:cd17647   386 GDCECCGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRRDKDEEPTLVSYIVPRFdkpddesfaqedvpkevstd 465

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  935 PVAP---------DALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAP 980
Cdd:cd17647   466 PIVKgligyrkliKDIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKLQFP 520
C_NRPS-like cd19066
Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of ...
 1082-1499 4.86e-64

Condensation domain of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long, with various activities such as antibiotic, antifungal, antitumor and immunosuppression. There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380453 [Multi-domain]  Cd Length: 427  Bit Score: 225.37  E-value: 4.86e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRiHPGPDGMPVQVADPAGGGAAL 1161
Cdd:cd19066     1 KIPLSPMQRGMWFLKKLATDPSAFNVAIEMFLTGSLDLARLKQALDAVMERHDVLRTR-FCEEAGRYEQVVLDKTVRFRI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPGTDLAQLLL----AEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTgH 1237
Cdd:cd19066    80 EIIDLRNLADPEARLLelidQIQQTIYDLERGPLVRVALFRLADERDVLVVAIHHIIVDGGSFQILFEDISSVYDAAE-R 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1238 REPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVAT-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIREL 1316
Cdd:cd19066   159 QKPTLPPPVGSYADYAAWLEKQLESEAAQADLAYWTSYLHGLPPPlPLPKAKRPSQVASYEVLTLEFFLRSEETKRLREV 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1317 ARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADA 1396
Cdd:cd19066   239 ARESGTTPTQLLLAAFALALKRLTASIDVVIGLTFLNRPDEAVEDTIGLFLNLLPLRIDTSPDATFPELLKRTKEQSREA 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1397 LAHQELPFEHLVAD----RGAGNGPLFRIMYSF-SSQEPAGRSAGDVDLEPVPVPVTTCKFDLVL---TVVDGGstLEGV 1468
Cdd:cd19066   319 IEHQRVPFIELVRHlgvvPEAPKHPLFEPVFTFkNNQQQLGKTGGFIFTTPVYTSSEGTVFDLDLeasEDPDGD--LLLR 396

                         410       420       430
                  ....*....|....*....|....*....|.
gi 653678460 1469 LEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19066   397 LEYSRGVYDERTIDRFAERYMTALRQLIENP 427
X-Domain_NRPS cd19546
X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to ...
 43-459 7.52e-61

X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to the non-ribosomal peptide synthetase (NRPS); The X-domain is a catalytically inactive member of the Condensation (C) domain family of non-ribosomal peptide synthetase (NRPS). It has been shown to recruit oxygenases to the NRPS to perform side-chain crosslinking in the production of glycopeptide antibiotics. C-domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as this X-domain, the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, and dual E/C (epimerization and condensation) domains. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity; members of this X-domain subfamily lack the second H of this motif.


Pssm-ID: 380468 [Multi-domain]  Cd Length: 440  Bit Score: 216.58  E-value: 7.52e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   43 PPGPVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDGEPVQQT--APAA 120
Cdd:cd19546     1 RPDEVPATAGQLRTWLLARLDEETRGRHLSVALRLRGRLDRDALEAALGDVAARHEILRTTFP-GDGGDVHQRIldADAA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  121 PVALPVIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY----AGE 196
Cdd:cd19546    80 RPELPVVPATEEELPALLADRAAHLFDLTRETPWRCTLFALSDTEHVLLLVVHRIAADDESLDVLVRDLAAAYgarrEGR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  197 LPAGVAAP-AFRDVAAWQHgRLAAGELD------DQLRYWRQRLAGLPD-LEIPGDRQRPADRDWRAHSVRRELPAATEA 268
Cdd:cd19546   160 APERAPLPlQFADYALWER-ELLAGEDDrdsligDQIAYWRDALAGAPDeLELPTDRPRPVLPSRRAGAVPLRLDAEVHA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  269 AVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR-GRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTV 347
Cdd:cd19546   239 RLMEAAESAGATMFTVVQAALAMLLTRLGAGTDVTVGTVLPRDdEEGDLEGMVGPFARPLALRTDLSGDPTFRELLGRVR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  348 AGSRNDFAHPQIPFDRLVRELRRDRIAGSQLLVQ-AMAGTESTAVALTFGEAAG-TEQPVDVALAKCDLDLSVL------ 419
Cdd:cd19546   319 EAVREARRHQDVPFERLAELLALPPSADRHPVFQvALDVRDDDNDPWDAPELPGlRTSPVPLGTEAMELDLSLAlterrn 398

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|..
gi 653678460  420 --DDGDRLAVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19546   399 ddGDPDGLDGSLRYAADLFDRATAAALARRLVRVLEQVAADP 440
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 524-977 1.04e-60

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 216.92  E-value: 1.04e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  524 ERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAA----YLPIDPDFPVERIAYLLSDARPVLVVTDAATA 599
Cdd:cd05922     1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADAGAA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  600 DRL------GPDDITgLVVLEETDTGGYPAtepPAVPAGHS--AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLT 671
Cdd:cd05922    81 DRLrdalpaSPDPGT-VLDADGIRAARASA---PAHEVSHEdlALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGIT 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  672 AEDRVLATTTVSFDIAGLELYLPLRSGAGVVLadADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPA-LAGIR 750
Cdd:cd05922   157 ADDRALTVLPLSYDYGLSVLNTHLLRGATLVL--TNDGVLDDAFWEDLREHGATGLAGVPSTYAMLTRLGFDPAkLPSLR 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  751 VL--VGGeALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLG 826
Cdd:cd05922   235 YLtqAGG-RLPQETIARLRELlpGAQVYVMYGQTEATRRMTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPG 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  827 ELHIAGEGLARGYWNRPGLTAEKflpdpfGPPGTRMYrTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINA 906
Cdd:cd05922   314 EIVHRGPNVMKGYWNDPPYRRKE------GRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSI 386

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460  907 GPVRRAAAVVREDRPGDlRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05922   387 GLIIEAAAVGLPDPLGE-KLALFVTAPDKIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAAL 456
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
 509-977 7.57e-60

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 213.48  E-value: 7.57e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  509 AVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDAR 588
Cdd:cd05919     3 AFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCE 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  589 PVLVVTDAatadrlgpDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMA-ER 667
Cdd:cd05919    83 ARLVVTSA--------DDI---------------------------AYLLYSSGTTGPPKGVMHAHRDPLLFADAMArEA 127

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  668 FPLTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLadADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGP-- 744
Cdd:cd05919   128 LGLTPGDRVFSSAKMFFGYGlGNSLWFPLAVGASAVL--NPGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSpd 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETT-IWSTSGPtseDSIRRGSIGVPIDNTQVYVLDANLHPAP 821
Cdd:cd05919   206 ALRSLRLCVsAGEALPRGLGERWMEHfGGPILDGIGATEVGhIFLSNRP---GAWRLGSTGRPVPGYEIRLVDEEGHTIP 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  822 IGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIET 901
Cdd:cd05919   283 PGEEGDLLVRGPSAAVGYWNNPEKSRATFNGG--------WYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVES 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  902 TLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPD-----ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:cd05919   355 LIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQeslarDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFK 434


                  .
gi 653678460  977 L 977
Cdd:cd05919   435 L 435
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
 497-977 1.44e-59

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 213.96  E-value: 1.44e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  497 FQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFP 576
Cdd:cd05936     5 LEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYT 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  577 VERIAYLLSDARPVLVVTDAATADRLGPdditglvvleetdtgGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAA 656
Cdd:cd05936    85 PRELEHILNDSGAKALIVAVSFTDLLAA---------------GAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRN 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  657 L-DNFLAAMA-ERFPLTAEDRVLATTTVsFDIAGLE--LYLPLRSGAGVVLAdadTARNPAALIDLAGRHRVTLAQATPT 732
Cdd:cd05936   150 LvANALQIKAwLEDLLEGDDVVLAALPL-FHVFGLTvaLLLPLALGATIVLI---PRFRPIGVLKEIRKHRVTIFPGVPT 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  733 LWQALV--PELSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPtSEDSIRRGSIGVPIDNT 808
Cdd:cd05936   226 MYIALLnaPEFKKRDFSSLRLCIsGGAPLPVEVAERFEELtGVPIVEGYGLTETSPVVAVNP-LDGPRKPGSIGIPLPGT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  809 QVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVK 888
Cdd:cd05936   305 EVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFVDGWL--------RTGDIGYMDEDGYFFIVDRKKDMII 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  889 LRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:cd05936   377 VGGFNVYPREVEEVLYEHPAVAEAAVVgVPDPYSGE-AVKAFVVlkEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELP 455

                         490
                  ....*....|..
gi 653678460  966 TTPNGKLDRAAL 977
Cdd:cd05936   456 KSAVGKILRREL 467
starter-C_NRPS cd19533
Starter Condensation domains, found in the first module of nonribosomal peptide synthetases ...
 48-459 2.16e-59

Starter Condensation domains, found in the first module of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. While standard C-domains catalyze peptide bond formation between two amino acids, an initial, ('starter') C-domain may instead acylate an amino acid with a fatty acid. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380456 [Multi-domain]  Cd Length: 419  Bit Score: 211.84  E-value: 2.16e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLgPDGEPVQQTAPAAPVALPVI 127
Cdd:cd19533     3 PLTSAQRGVWFAEQLDPEGSIYNLAEYLEITGPVDLAVLERALRQVIAEAETLRLRFTE-EEGEPYQWIDPYTPVPIRHI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  128 DVTEADLPQA-----LRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLL---LADLARAYAGELPA 199
Cdd:cd19533    82 DLSGDPDPEGaaqqwMQEDLRKPLPLDNDPLFRHALFTLGDNRHFWYQRVHHIVMDGFSFALFgqrVAEIYTALLKGRPA 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  200 GvaAPAFRDVAAWQHGRLAAGELDDQLR---YWRQRLAGLPDLEIPGDRQRPADRDWRAHSVrrELPAATEAAVRRLAAA 276
Cdd:cd19533   162 P--PAPFGSFLDLVEEEQAYRQSERFERdraFWTEQFEDLPEPVSLARRAPGRSLAFLRRTA--ELPPELTRTLLEAAEA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  277 HDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDFAH 356
Cdd:cd19533   238 HGASWPSFFIALVAAYLHRLTGANDVVLGVPVMGRLGAAARQTPGMVANTLPLRLTVDPQQTFAELVAQVSRELRSLLRH 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  357 PQIPFDRLVRELRR----DRIAGSQLLVQAMagtestAVALTFGEAAGTEQ-----PVDvalakcDLDLSVLD--DGDRL 425
Cdd:cd19533   318 QRYRYEDLRRDLGLtgelHPLFGPTVNYMPF------DYGLDFGGVVGLTHnlssgPTN------DLSIFVYDrdDESGL 385

                         410       420       430
                  ....*....|....*....|....*....|....
gi 653678460  426 AVTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19533   386 RIDFDANPALYSGEDLARHQERLLRLLEEAAADP 419
C_PKS-NRPS cd19532
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1084-1499 3.29e-59

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHxxxD motif, a few such as Monascus pilosus lovastatin nonaketide synthase MokA have a non-canonical HRxxxD motif in the C-domain and are unable to catalyze amide-bond formation. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380455 [Multi-domain]  Cd Length: 421  Bit Score: 211.16  E-value: 3.29e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGP-DGMPVQV--ADPAgggAA 1160
Cdd:cd19532     3 PMSFGQSRFWFLQQYLEDPTTFNVTFSYRLTGPLDVARLERAVRAVGQRHEALRTCFFTDPeDGEPMQGvlASSP---LR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1161 LTERDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTghrep 1240
Cdd:cd19532    80 LEHVQISDEAEVEEEFERLKNHVYDLESGETMRIVLLSLSPTEHYLIFGYHHIAMDGVSFQIFLRDLERAYNGQP----- 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1241 eLDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATevagdLP---------RPATPGGGGGAVEFALAPRQIQ 1311
Cdd:cd19532   155 -LLPPPLQYLDFAARQRQDYESGALDEDLAYWKSEFSTLPEP-----LPllpfakvksRPPLTRYDTHTAERRLDAALAA 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1312 GIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHA 1391
Cdd:cd19532   229 RIKEASRKLRVTPFHFYLAALQVLLARLLDVDDICIGIADANRTDEDFMETIGFFLNLLPLRFRRDPSQTFADVLKETRD 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1392 RTADALAHQELPF----EHLVADRGAGNGPLFRIMYSFSSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVD---GGST 1464
Cdd:cd19532   309 KAYAALAHSRVPFdvllDELGVPRSATHSPLFQVFINYRQGVAESRPFGDCELEGEEFEDARTPYDLSLDIIDnpdGDCL 388

                         410       420       430
                  ....*....|....*....|....*....|....*
gi 653678460 1465 LEgvLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19532   389 LT--LKVQSSLYSEEDAELLLDSYVNLLEAFARDP 421
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
 514-977 1.69e-55

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 201.16  E-value: 1.69e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  514 DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARpvlvV 593
Cdd:cd17654    14 DTTVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQRSLTVMKKCH----V 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAATADRLGpdditglvVLEETDTggyPATEPPAVPAGHS-AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTA 672
Cdd:cd17654    90 SYLLQNKELD--------NAPLSFT---PEHRHFNIRTDEClAYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITS 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  673 EDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALID-LAGRHRVTLAQATPTLWQALVPELSGP----ALA 747
Cdd:cd17654   159 EDILFLTSPLTFDPSVVEIFLSLSSGATLLIVPTSVKVLPSKLADiLFKRHRITVLQATPTLFRRFGSQSIKStvlsATS 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  748 GIRVL-VGGEALPAEL---ARRLGDAGAEVTNMYGPTETTIWSTSG--PTSEDSIRrgsIGVPIDNTQVYVLDANLHPap 821
Cdd:cd17654   239 SLRVLaLGGEPFPSLVilsSWRGKGNRTRIFNIYGITEVSCWALAYkvPEEDSPVQ---LGSPLLGTVIEVRDQNGSE-- 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  822 igVLGELHIagEGLARGYwnrpgltaekFLPDPFGPPGTRMYRTGDLVRwSAAGDLEYLGRTDHQVKLRGFRIELGEIET 901
Cdd:cd17654   314 --GTGQVFL--GGLNRVC----------ILDDEVTVPKGTMRATGDFVT-VKDGELFFLGRKDSQIKRRGKRINLDLIQQ 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  902 TLINAGPVrRAAAVVREDrpgDLRLVAYVI---PDGPVAPDALRTELSRT-LPDYMipaviVPVPDFPTTPNGKLDRAAL 977
Cdd:cd17654   379 VIESCLGV-ESCAVTLSD---QQRLIAFIVgesSSSRIHKELQLTLLSSHaIPDTF-----VQIDKLPLTSHGKVDKSEL 449
C_PKS-NRPS cd20483
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 46-453 2.39e-55

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHXXXD motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380471 [Multi-domain]  Cd Length: 430  Bit Score: 200.18  E-value: 2.39e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLG-PDGEpvQQTAPAAPVAL 124
Cdd:cd20483     1 PRPMSTFQRRLWFLHNFLEDKTFLNLLLVCHIKGKPDVNLLQKALSELVRRHEVLRTAYFEGdDFGE--QQVLDDPSFHL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  125 PVIDVT-----EADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY----AG 195
Cdd:cd20483    79 IVIDLSeaadpEAALDQLVRNLRRQELDIEEGEVIRGWLVKLPDEEFALVLASHHIAWDRGSSKSIFEQFTALYdalrAG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  196 ELPAGVAAPA--FRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPDLE--IP-GDRQRPADRDWRAHSVRRELPAATEAAV 270
Cdd:cd20483   159 RDLATVPPPPvqYIDFTLWHNALLQSPLVQPLLDFWKEKLEGIPDASklLPfAKAERPPVKDYERSTVEATLDKELLARM 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  271 RRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGS 350
Cdd:cd20483   239 KRICAQHAVTPFMFLLAAFRAFLYRYTEDEDLTIGMVDGDRPHPDFDDLVGFFVNMLPIRCRMDCDMSFDDLLESTKTTC 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  351 RNDFAHPQIPFDRLVRELRRDRIAG----SQLLVQ-AMAGTESTAvalTFGEAAGTEQPVDVALAKCDLDLSVLDDGDR- 424
Cdd:cd20483   319 LEAYEHSAVPFDYIVDALDVPRSTShfpiGQIAVNyQVHGKFPEY---DTGDFKFTDYDHYDIPTACDIALEAEEDPDGg 395

                         410       420
                  ....*....|....*....|....*....
gi 653678460  425 LAVTLVAAADLFTPAAAEQLAGQFLSLLA 453
Cdd:cd20483   396 LDLRLEFSTTLYDSADMERFLDNFVTFLT 424
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
 497-977 3.95e-55

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 202.34  E-value: 3.95e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  497 FQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFP 576
Cdd:PRK06187   12 LRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIRLK 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  577 VERIAYLLSDARPVLVVTDAATADRLGP-----DDITGLVVLEETDTGGY--------------PATEPPAVPAGHSAYV 637
Cdd:PRK06187   92 PEEIAYILNDAEDRVVLVDSEFVPLLAAilpqlPTVRTVIVEGDGPAAPLapevgeyeellaaaSDTFDFPDIDENDAAA 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  638 -IYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVsFDIAGLEL-YLPLRSGAGVVLADadtaR-NPAA 714
Cdd:PRK06187  172 mLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDDVYLVIVPM-FHVHAWGLpYLALMAGAKQVIPR----RfDPEN 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  715 LIDLAGRHRVTLAQATPTLWQALVPELSGPA--LAGIR-VLVGGEALPAELARR-LGDAGAEVTNMYGPTETT-IWSTSG 789
Cdd:PRK06187  247 LLDLIETERVTFFFAVPTIWQMLLKAPRAYFvdFSSLRlVIYGGAALPPALLREfKEKFGIDLVQGYGMTETSpVVSVLP 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSED---SIRRGSIGVPIDNTQVYVLDANLHPAP--IGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYR 864
Cdd:PRK06187  327 PEDQLpgqWTKRRSAGRPLPGVEARIVDDDGDELPpdGGEVGEIIVRGPWLMQGYWNRPEATAETIDGG--------WLH 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  865 TGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIEtTLINAGPVRRAAAV--VREDRPGDlRLVAYVI--PDGPVAPDA 940
Cdd:PRK06187  399 TGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELE-DALYGHPAVAEVAVigVPDEKWGE-RPVAVVVlkPGATLDAKE 476

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 653678460  941 LRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06187  477 LRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
DCL_NRPS cd19543
DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the ...
 1083-1499 8.54e-55

DCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs), which catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor; The DCL-type Condensation (C) domain catalyzes the condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. This domain is D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains in addition to the LCL- and DCL-types such as starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380465 [Multi-domain]  Cd Length: 423  Bit Score: 198.58  E-value: 8.54e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1083 LPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADpAGGGAALT 1162
Cdd:cd19543     2 YPLSPMQEGMLFHSLLDPGSGAYVEQMVITLEGPLDPDRFRAAWQAVVDRHPILRTSFVWEGLGEPLQVVL-KDRKLPWR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1163 ERDAAPGTDLAQLLLAEA------ALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTG 1236
Cdd:cd19543    81 ELDLSHLSEAEQEAELEAlaeedrERGFDLARAPLMRLTLIRLGDDRYRLVWSFHHILLDGWSLPILLKELFAIYAALGE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1237 HREPELdEPALAQADYAVW-QRQSAQrgryAAElDFWRTELTGAVA-TEVAGDLPRPATPGGGGGAVEFALAPRQIQGIR 1314
Cdd:cd19543   161 GQPPSL-PPVRPYRDYIAWlQRQDKE----AAE-AYWREYLAGFEEpTPLPKELPADADGSYEPGEVSFELSAELTARLQ 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1315 ELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHAR 1392
Cdd:cd19543   235 ELARQHGVTLNTVVQGAWALLLSRYSGRDDVVFGTTVSGRpaELPGIETMVGLFINTLPVRVRLDPDQTVLELLKDLQAQ 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1393 TADALAHQELPFehlvAD---RGAGNGPLF-RIM----YSFSSQEPAGRSAGDVDLEPVPVPVTTcKFDLVLTVVDGGsT 1464
Cdd:cd19543   315 QLELREHEYVPL----YEiqaWSEGKQALFdHLLvfenYPVDESLEEEQDEDGLRITDVSAEEQT-NYPLTVVAIPGE-E 388

                         410       420       430
                  ....*....|....*....|....*....|....*
gi 653678460 1465 LEGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19543   389 LTIKLSYDAEVFDEATIERLLGHLRRVLEQVAANP 423
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
 505-977 2.93e-54

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 199.52  E-value: 2.93e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd05959    18 GDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLGP---DDITGLVVLEETDTGG----------YPATEPPAVPAGHS-----AYVIYTSGSTGR 646
Cdd:cd05959    98 EDSRARVVVVSGELAPVLAAaltKSEHTLVVLIVSGGAGpeagalllaeLVAAEAEQLKPAAThaddpAFWLYSSGSTGR 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  647 PKGVVITRAALdnflAAMAERFP-----LTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLADADTArnPAALIDLAG 720
Cdd:cd05959   178 PKGVVHLHADI----YWTAELYArnvlgIREDDVCFSAAKLFFAYGlGNSLTFPLSVGATTVLMPERPT--PAAVFKRIR 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQATPTLWQAL--VPELSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETT-IWSTSGPtseDS 795
Cdd:cd05959   252 RYRPTVFFGVPTLYAAMlaAPNLPSRDLSSLRLCVsAGEALPAEVGERWKARfGLDILDGIGSTEMLhIFLSNRP---GR 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  796 IRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLpdpfGPpgtrMYRTGDLVRWSAAG 875
Cdd:cd05959   329 VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDTFQ----GE----WTRTGDKYVRDDDG 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  876 DLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP-DGPVAPDALRTELSR----TLP 950
Cdd:cd05959   401 FYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLrPGYEDSEALEEELKEfvkdRLA 480

                         490       500
                  ....*....|....*....|....*..
gi 653678460  951 DYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05959   481 PYKYPRWIVFVDELPKTATGKIQRFKL 507
X-Domain_NRPS cd19546
X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to ...
 1079-1492 5.75e-53

X-domain is a catalytically inactive Condensation-like domain shown to recruit oxygenases to the non-ribosomal peptide synthetase (NRPS); The X-domain is a catalytically inactive member of the Condensation (C) domain family of non-ribosomal peptide synthetase (NRPS). It has been shown to recruit oxygenases to the NRPS to perform side-chain crosslinking in the production of glycopeptide antibiotics. C-domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as this X-domain, the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, and dual E/C (epimerization and condensation) domains. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity; members of this X-domain subfamily lack the second H of this motif.


Pssm-ID: 380468 [Multi-domain]  Cd Length: 440  Bit Score: 193.85  E-value: 5.75e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1079 RDEPLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIhPGPDGMPVQ-VADPAGG 1157
Cdd:cd19546     1 RPDEVPATAGQLRTWLLARLDEETRGRHLSVALRLRGRLDRDALEAALGDVAARHEILRTTF-PGDGGDVHQrILDADAA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1158 GAALTERDAAPgTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGH 1237
Cdd:cd19546    80 RPELPVVPATE-EELPALLADRAAHLFDLTRETPWRCTLFALSDTEHVLLLVVHRIAADDESLDVLVRDLAAAYGARREG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1238 REPELDEPALAQADYAVWQRQ-----SAQRGRYAAELDFWRTELTGAV-ATEVAGDLPRPATPGGGGGAVEFALAPRQIQ 1311
Cdd:cd19546   159 RAPERAPLPLQFADYALWEREllageDDRDSLIGDQIAYWRDALAGAPdELELPTDRPRPVLPSRRAGAVPLRLDAEVHA 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1312 GIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR-GNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTH 1390
Cdd:cd19546   239 RLMEAAESAGATMFTVVQAALAMLLTRLGAGTDVTVGTVLPRDdEEGDLEGMVGPFARPLALRTDLSGDPTFRELLGRVR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1391 ARTADALAHQELPFEHLVADRG----AGNGPLFRIMYSFSSQEPAGRSAGDV---DLEPVPVPVTTCKFDLVLTVVD--- 1460
Cdd:cd19546   319 EAVREARRHQDVPFERLAELLAlppsADRHPVFQVALDVRDDDNDPWDAPELpglRTSPVPLGTEAMELDLSLALTErrn 398

                         410       420       430
                  ....*....|....*....|....*....|....*
gi 653678460 1461 ---GGSTLEGVLEYADDLYLPGTAERLVTSLTNLL 1492
Cdd:cd19546   399 ddgDPDGLDGSLRYAADLFDRATAAALARRLVRVL 433
COG4908 COG4908
Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General ...
 1085-1324 3.17e-52

Uncharacterized conserved protein, contains a NRPS condensation (elongation) domain [General function prediction only];


Pssm-ID: 443936 [Multi-domain]  Cd Length: 243  Bit Score: 184.86  E-value: 3.17e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1085 LSPMQESIWLADQvsaGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGpDGMPVQVADPAGGgAALTER 1164
Cdd:COG4908     1 LSPAQKRFLFLEP---GSNAYNIPAVLRLEGPLDVEALERALRELVRRHPALRTRFVEE-DGEPVQRIDPDAD-LPLEVV 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1165 D------AAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHR 1238
Cdd:COG4908    76 DlsalpePEREAELEELVAEEASRPFDLARGPLLRAALIRLGEDEHVLLLTIHHIISDGWSLGILLRELAALYAALLEGE 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1239 EPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAV-ATEVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELA 1317
Cdd:COG4908   156 PPPLPELPIQYADYAAWQRAWLQSEALEKQLEYWRQQLAGAPpVLELPTDRPRPAVQTFRGATLSFTLPAELTEALKALA 235


                  ....*..
gi 653678460 1318 RRCGTTV 1324
Cdd:COG4908   236 KAHGATV 242
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
 507-977 9.70e-52

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 190.19  E-value: 9.70e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  507 AVAVIEGDTTLSYRELDERANRLARLLMERGAGAE-TFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLS 585
Cdd:cd05941     2 RIAIVDDGDSITYADLVARAARLANRLLALGKDLRgDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVIT 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  586 DARPVLVVTDAAtadrlgpdditglvvleetdtggypateppavpaghsayVIYTSGSTGRPKGVVITRAALDNFLAAMA 665
Cdd:cd05941    82 DSEPSLVLDPAL---------------------------------------ILYTSGTTGRPKGVVLTHANLAANVRALV 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  666 ERFPLTAEDRVLATTTVsFDIAGLELYL--PLRSGAGVVLADADTARNPAALIDLAgrhRVTLAQATPTLWQALV--PEL 741
Cdd:cd05941   123 DAWRWTEDDVLLHVLPL-HHVHGLVNALlcPLFAGASVEFLPKFDPKEVAISRLMP---SITVFMGVPTIYTRLLqyYEA 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  742 SGP--------ALAGIRVLVGGEA-LPAELARRLGDA-GAEVTNMYGPTETTIwSTSGPTSEDsiRR-GSIGVPIDNTQV 810
Cdd:cd05941   199 HFTdpqfaraaAAERLRLMVSGSAaLPVPTLEEWEAItGHTLLERYGMTEIGM-ALSNPLDGE--RRpGTVGMPLPGVQA 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  811 YVLDAN-LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRT-DHQVK 888
Cdd:cd05941   276 RIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW-------FKTGDLGVVDEDGYYWILGRSsVDIIK 348

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  889 LRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAP---DALRTELSRTLPDYMIPAVIVPVPDFP 965
Cdd:cd05941   349 SGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGAAAlslEELKEWAKQRLAPYKRPRRLILVDELP 428

                         490
                  ....*....|..
gi 653678460  966 TTPNGKLDRAAL 977
Cdd:cd05941   429 RNAMGKVNKKEL 440
CT_NRPS-like cd19542
Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike ...
 1083-1499 2.46e-51

Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike bacterial NRPS, which typically have specialized terminal thioesterase (TE) domains to cyclize peptide products, many fungal NRPSs employ a terminal condensation-like (CT) domain to produce macrocyclic peptidyl products (e.g. cyclosporine and echinocandin). Domains in this subfamily (which includes both terminal and non-terminal domains) typically have a non-canonical conserved [SN]HxxxDx(14)Y motif at their active site compared to the standard Condensation (C) domain active site motif (HHxxxD). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380464 [Multi-domain]  Cd Length: 401  Bit Score: 187.51  E-value: 2.46e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1083 LPLSPMQESIwLADQVSAGDSvYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTR-IHPGPDGMPVQVadpagggaal 1161
Cdd:cd19542     2 YPCTPMQEGM-LLSQLRSPGL-YFNHFVFDLDSSVDVERLRNAWRQLVQRHDILRTVfVESSAEGTFLQV---------- 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPG-------TDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYaad 1234
Cdd:cd19542    70 VLKSLDPPieevetdEDSLDALTRDLLDDPTLFGQPPHRLTLLETSSGEVYLVLRISHALYDGVSLPIILRDLAAAY--- 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1235 tghrEPELDEPALAQADYAvwqrQSAQRGRYAAELDFWRTELTGAVATEVagdlprPATPGGGGGAVEFALAPRQIQGIR 1314
Cdd:cd19542   147 ----NGQLLPPAPPFSDYI----SYLQSQSQEESLQYWRKYLQGASPCAF------PSLSPKRPAERSLSSTRRSLAKLE 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1315 ELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPR--LDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHAR 1392
Cdd:cd19542   213 AFCASLGVTLASLFQAAWALVLARYTGSRDVVFGYVVSGRDLPVpgIDDIVGPCINTLPVRVKLDPDWTVLDLLRQLQQQ 292

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1393 TADALAHQELPFEHLV-ADRGAGNGPLFRIMYSF----SSQEPAGRSAGDVDLEPVPVPVTtckFDLVLTVVDGGSTLEG 1467
Cdd:cd19542   293 YLRSLPHQHLSLREIQrALGLWPSGTLFNTLVSYqnfeASPESELSGSSVFELSAAEDPTE---YPVAVEVEPSGDSLKV 369

                         410       420       430
                  ....*....|....*....|....*....|..
gi 653678460 1468 VLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19542   370 SLAYSTSVLSEEQAEELLEQFDDILEALLANP 401
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 518-978 2.55e-51

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 188.27  E-value: 2.55e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  518 SYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDaa 597
Cdd:cd05934     5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVVD-- 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  598 tadrlgpdditglvvleetdtggypateppavpaghSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDrVL 677
Cdd:cd05934    83 ------------------------------------PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFGLGEDD-VY 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  678 ATTTVSFDIAGLE--LYLPLRSGAGVVLADADTARnpaALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRVLV 753
Cdd:cd05934   126 LTVLPLFHINAQAvsVLAALSVGATLVLLPRFSAS---RFWSDVRRYGATVTNYLGAMLSYLLaqPPSPDDRAHRLRAAY 202

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  754 GGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPtsEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE 833
Cdd:cd05934   203 GAPNPPELHEEFEERFGVRLLEGYGMTETIVGVIGPR--DEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRGL 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  834 ---GLARGYWNRPGLTAEKFlpdpfgPPGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVR 910
Cdd:cd05934   281 rgwGFFKGYYNMPEATAEAM------RNG--WFHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVR 352

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  911 RAAAV-VREDRPGDLRLVAYVIPDG-PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALP 978
Cdd:cd05934   353 EAAVVaVPDEVGEDEVKAVVVLRPGeTLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
 1685-2028 1.11e-50

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 183.64  E-value: 1.11e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1685 LAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMP---ADATL 1761
Cdd:cd04433     2 PALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPkfdPEAAL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1762 ADLAEElsGPlayDYIRLTPSHLRHLVGHWTGQELP-PAARGWVVGGETLDPALVKQLLElRPDAEVINHYGPTETvIGR 1840
Cdd:cd04433    82 ELIERE--KV---TILLGVPTLLARLLKAPESAGYDlSSLRALVSGGAPLPPELLERFEE-APGIKLVNGYGLTET-GGT 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1841 VVHPVREAGELAVDSplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpagEPGarM 1920
Cdd:cd04433   155 VATGPPDDDARKPGS---VGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVD------EDG--W 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1921 YRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQQ----LVGWFIPAEDHPPvTVS 1995
Cdd:cd04433   224 YRTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEaAVVGVPDPEwgerVVAVVVLRPGADL-DAE 302

                         330       340       350
                  ....*....|....*....|....*....|...
gi 653678460 1996 ALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd04433   303 ELRAHVRERLAPYKVPRRVVFVDALPRTASGKI 335
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
 495-977 6.25e-50

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 187.28  E-value: 6.25e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAylpidpd 574
Cdd:COG1021    29 DLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI------- 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 fPV---------ErIAYLLSDARPVLVVTDA--------ATADRL--------------GPDDITGLVVLEETDTGGypa 623
Cdd:COG1021   102 -PVfalpahrraE-ISHFAEQSEAVAYIIPDrhrgfdyrALARELqaevpslrhvlvvgDAGEFTSLDALLAAPADL--- 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  624 tEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTV-------SFDIAGlelylPLR 696
Cdd:COG1021   177 -SEPRPDPDDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRASAEICGLDADTVYLAALPAahnfplsSPGVLG-----VLY 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  697 SGAGVVLADADTARNPAALIDlagRHRVTLAQATPTLWQALV--PELSGPALAGIRVL-VGGEALPAELARRLGDA-GAE 772
Cdd:COG1021   251 AGGTVVLAPDPSPDTAFPLIE---RERVTVTALVPPLALLWLdaAERSRYDLSSLRVLqVGGAKLSPELARRVRPAlGCT 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  773 VTNMYGPTETTIWSTSGPTSEDsIRRGSIGVPI-DNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFL 851
Cdd:COG1021   328 LQQVFGMAEGLVNYTRLDDPEE-VILTTQGRPIsPDDEVRIVDEDGNPVPPGEVGELLTRGPYTIRGYYRAPEHNARAFT 406

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  852 PDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIEtTLINAGP-VRRAAAVVREDRpgDL--RLVA 928
Cdd:COG1021   407 PDGF-------YRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVE-NLLLAHPaVHDAAVVAMPDE--YLgeRSCA 476

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460  929 YVIPDG-PVAPDALRTEL-SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:COG1021   477 FVVPRGePLTLAELRRFLrERGLAAFKLPDRLEFVDALPLTAVGKIDKKAL 527
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
 507-977 1.75e-49

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 184.82  E-value: 1.75e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  507 AVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSD 586
Cdd:cd05926     5 ALVVPGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLAD 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  587 ARPVLVVTDA--------------ATADRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHS-----AYVIYTSGSTGRP 647
Cdd:cd05926    85 LGSKLVLTPKgelgpasraasklgLAILELALDVGVLIRAPSAESLSNLLADKKNAKSEGVPlpddlALILHTSGTTGRP 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  648 KGVVITRAaldNFLAAM---AERFPLTAEDRVLATTTVsFDIAGL--ELYLPLRSGAGVVLADADTArnpAALIDLAGRH 722
Cdd:cd05926   165 KGVPLTHR---NLAASAtniTNTYKLTPDDRTLVVMPL-FHVHGLvaSLLSTLAAGGSVVLPPRFSA---STFWPDVRDY 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLWQALV---PELSGPALAGIRVL-VGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIR 797
Cdd:cd05926   238 NATWYTAVPTIHQILLnrpEPNPESPPPKLRFIrSCSASLPPAVLEALEATfGAPVLEAYGMTEAAHQMTSNPLPPGPRK 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  798 RGSIGVPiDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDL 877
Cdd:cd05926   318 PGSVGKP-VGVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGW-------FRTGDLGYLDADGYL 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  878 EYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLrLVAYVIPDG--PVAPDALRTELSRTLPDYMI 954
Cdd:cd05926   390 FLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFgVPDEKYGEE-VAAAVVLREgaSVTEEELRAFCRKHLAAFKV 468

                         490       500
                  ....*....|....*....|...
gi 653678460  955 PAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05926   469 PKKVYFVDELPKTATGKIQRRKV 491
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
 1555-2036 1.89e-48

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 182.72  E-value: 1.89e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1555 RSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTP 1634
Cdd:cd17647    19 RSFTYRDINEASNIVAHYLIKTGIKRGDV-----VMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKP 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1635 -ALVLTRtgQGGCLPGaevfgdvpvvaldrvadrltamPDQRPEVTvdprglaysiFTSGSTGQPKGVAVEHIGIVRYLS 1713
Cdd:cd17647    94 rGLIVIR--AAGVVVG----------------------PDSNPTLS----------FTSGSEGIPKGVLGRHFSLAYYFP 139

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1714 WAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPAD-----ATLADLAEELSGPLAYdyirLTPSHLRHLV 1788
Cdd:cd17647   140 WMAKRFNLSENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTQDdigtpGRLAEWMAKYGATVTH----LTPAMGQLLT 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1789 GHWTGQeLPPAARGWVVGgETLDPALVKQLLELRPDAEVINHYGPTET--VIGRVVHPVREAGELAVDS---PLPLGRPL 1863
Cdd:cd17647   216 AQATTP-FPKLHHAFFVG-DILTKRDCLRLQTLAENVRIVNMYGTTETqrAVSYFEVPSRSSDPTFLKNlkdVMPAGRGM 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1864 GETRLQVLDAW--LEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLP------------DPAGE---------PGARM 1920
Cdd:cd17647   294 LNVQLLVVNRNdrTQICGIGEVGEIYVRAGGLAEGYRGLPELNKEKFVNnwfvepdhwnylDKDNNepwrqfwlgPRDRL 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1921 YRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQ-----LVGWFIPAEDHPPVTVS 1995
Cdd:cd17647   374 YRTGDLGRYLPNGDCECCGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRRDKdeeptLVSYIVPRFDKPDDESF 453

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1996 A--------------------------LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:cd17647   454 AqedvpkevstdpivkgligyrklikdIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKLQFP 520
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
 516-972 2.03e-48

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 180.27  E-value: 2.03e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP-VLVVT 594
Cdd:cd05903     1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAkVFVVP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 daataDRLGPDDitglvvleetdtggyPATEPPAVpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAED 674
Cdd:cd05903    81 -----ERFRQFD---------------PAAMPDAV-----ALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGLGPGD 135

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  675 RVLATTTVSFDIAGLE-LYLPLRSGAGVVLADADTARNPAALIDlagRHRVTLAQATPTLWQAL--VPELSGPALAGIR- 750
Cdd:cd05903   136 VFLVASPMAHQTGFVYgFTLPLLLGAPVVLQDIWDPDKALALMR---EHGVTFMMGATPFLTDLlnAVEEAGEPLSRLRt 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  751 VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELH 829
Cdd:cd05903   213 FVCGGATVPRSLARRAAELlGAKVCSAYGSTECPGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELL 292

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  830 IAGEGLARGYWNRPGLTAEkFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPV 909
Cdd:cd05903   293 SRGPSVFLGYLDRPDLTAD-AAPEGW-------FRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGV 364

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  910 RRAAAVVREDRPGDLRLVAYVIPDGPVAP--DALRTELSRT-LPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:cd05903   365 IEAAVVALPDERLGERACAVVVTKSGALLtfDELVAYLDRQgVAKQYWPERLVHVDDLPRTPSGKV 430
CT_NRPS-like cd19542
Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike ...
 48-459 7.82e-48

Terminal Condensation (CT)-like domains of nonribosomal peptide synthetases (NRPSs); Unlike bacterial NRPS, which typically have specialized terminal thioesterase (TE) domains to cyclize peptide products, many fungal NRPSs employ a terminal condensation-like (CT) domain to produce macrocyclic peptidyl products (e.g. cyclosporine and echinocandin). Domains in this subfamily (which includes both terminal and non-terminal domains) typically have a non-canonical conserved [SN]HxxxDx(14)Y motif at their active site compared to the standard Condensation (C) domain active site motif (HHxxxD). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380464 [Multi-domain]  Cd Length: 401  Bit Score: 177.50  E-value: 7.82e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIwFSEQLRPGtPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVF-RLGPDGEPVQQTAPAAPVALPV 126
Cdd:cd19542     3 PCTPMQEGM-LLSQLRSP-GLYFNHFVFDLDSSVDVERLRNAWRQLVQRHDILRTVFvESSAEGTFLQVVLKSLDPPIEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  127 IDVTEADLPQALRTAAEQPFDLERgPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGELPAgvAAPAF 206
Cdd:cd19542    81 VETDEDSLDALTRDLLDDPTLFGQ-PPHRLTLLETSSGEVYLVLRISHALYDGVSLPIILRDLAAAYNGQLLP--PAPPF 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  207 RDVAAwqhgRLAAGELDDQLRYWRQRLAGLPDLEIPG-DRQRPADRdwRAHSVRRELpaateAAVRRLAAAHDTTPFTVL 285
Cdd:cd19542   158 SDYIS----YLQSQSQEESLQYWRKYLQGASPCAFPSlSPKRPAER--SLSSTRRSL-----AKLEAFCASLGVTLASLF 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  286 LAAYGTFLHRLTGQDDFAVGVPVAGRG--RTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDFAHPQIPFDR 363
Cdd:cd19542   227 QAAWALVLARYTGSRDVVFGYVVSGRDlpVPGIDDIVGPCINTLPVRVKLDPDWTVLDLLRQLQQQYLRSLPHQHLSLRE 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  364 LVRELRRDRIA---GSQLLVQAMAGTESTAVALTFGEAAGTEQ-PVDVALAkcdldLSVLDDGDRLAVTLVAAADLFTPA 439
Cdd:cd19542   307 IQRALGLWPSGtlfNTLVSYQNFEASPESELSGSSVFELSAAEdPTEYPVA-----VEVEPSGDSLKVSLAYSTSVLSEE 381

                         410       420
                  ....*....|....*....|
gi 653678460  440 AAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19542   382 QAEELLEQFDDILEALLANP 401
PRK07787 PRK07787
acyl-CoA synthetase; Validated
 509-980 1.41e-47

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 178.64  E-value: 1.41e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  509 AVIEGDTTLSYRELDERANRLArllmERGAGAETfVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDAR 588
Cdd:PRK07787   18 AVRIGGRVLSRSDLAGAATAVA----ERVAGARR-VAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADSG 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  589 PVLVVTDAatadrlgPDDITGLVVlEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERF 668
Cdd:PRK07787   93 AQAWLGPA-------PDDPAGLPH-VPVRLHARSWHRYPEPDPDAPALIVYTSGTTGPPKGVVLSRRAIAADLDALAEAW 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  669 PLTAEDrVLATTTVSFDIAGLELYL--PLRSGAGVVLADADTARNPAALIDLAGrhrvTLAQATPTLWQALV--PELSGp 744
Cdd:PRK07787  165 QWTADD-VLVHGLPLFHVHGLVLGVlgPLRIGNRFVHTGRPTPEAYAQALSEGG----TLYFGVPTVWSRIAadPEAAR- 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGIRVLVGGEA-LPAELARRLGDA-GAEVTNMYGPTETTIwSTSgpTSEDSIRR-GSIGVPIDNTQVYVLDANLHPAP 821
Cdd:PRK07787  239 ALRGARLLVSGSAaLPVPVFDRLAALtGHRPVERYGMTETLI-TLS--TRADGERRpGWVGLPLAGVETRLVDEDGGPVP 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  822 IGV--LGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGR--TDhQVKLRGFRIELG 897
Cdd:PRK07787  316 HDGetVGELQVRGPTLFDGYLNRPDATAAAFTADGW-------FRTGDVAVVDPDGMHRIVGResTD-LIKSGGYRIGAG 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  898 EIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07787  388 EIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQL 467


                  ...
gi 653678460  978 PAP 980
Cdd:PRK07787  468 LSE 470
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
 502-977 7.15e-47

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 177.79  E-value: 7.15e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PRK07656   16 RRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTADEAA 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLL--SDARPVLVVTDAATADRLGPDDITGL--VVLEETDTG----------------GYPATEPPAVPAGHSAYVIYTS 641
Cdd:PRK07656   96 YILarGDAKALFVLGLFLGVDYSATTRLPALehVVICETEEDdphtekmktftdflaaGDPAERAPEVDPDDVADILFTS 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  642 GSTGRPKGVVIT-RAALDNFlAAMAERFPLTAEDRVLATTTVsFDIAGLELYL--PLRSGAGVVLAdadTARNPAALIDL 718
Cdd:PRK07656  176 GTTGRPKGAMLThRQLLSNA-ADWAEYLGLTEGDRYLAANPF-FHVFGYKAGVnaPLMRGATILPL---PVFDPDEVFRL 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  719 AGRHRVTLAQATPTLWQAL--VPELSGPALAGIRVLV-GGEALPAELARRLGDA-GAE-VTNMYGPTETTIWSTSGPTSE 793
Cdd:PRK07656  251 IETERITVLPGPPTMYNSLlqHPDRSAEDLSSLRLAVtGAASMPVALLERFESElGVDiVLTGYGLSEASGVTTFNRLDD 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  794 D-SIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWS 872
Cdd:PRK07656  331 DrKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATAAAIDADGW-------LHTGDLGRLD 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  873 AAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLrLVAYVI--PDGPVAPDALRTELSRTL 949
Cdd:PRK07656  404 EEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIgVPDERLGEV-GKAYVVlkPGAELTEEELIAYCREHL 482

                         490       500
                  ....*....|....*....|....*...
gi 653678460  950 PDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07656  483 AKYKVPRSIEFLDELPKNATGKVLKRAL 510
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
 492-977 5.96e-46

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 174.44  E-value: 5.96e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  492 SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPI 571
Cdd:cd05920    16 PLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLA 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  572 DPDFPVERIAYLLSDARPVLVVTDaataDRLGPDDITGLVVLEetdtggypATEPPAVpaghsAYVIYTSGSTGRPKGVV 651
Cdd:cd05920    96 LPSHRRSELSAFCAHAEAVAYIVP----DRHAGFDHRALAREL--------AESIPEV-----ALFLLSGGTTGTPKLIP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAALDNFLAAMAERFPLTAEDRVLATTTVS--FDIAGLELYLPLRSGAGVVLADADTARNPAALIDlagRHRVTLAQA 729
Cdd:cd05920   159 RTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAhnFPLACPGVLGTLLAGGRVVLAPDPSPDAAFPLIE---REGVTVTAL 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  730 TPTLWQALVPEL--SGPALAGIRVL-VGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIrRGSIGVPI 805
Cdd:cd05920   236 VPALVSLWLDAAasRRADLSSLRLLqVGGARLSPALARRVPPVlGCTLQQVFGMAEGLLNYTRLDDPDEVI-IHTQGRPM 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  806 D-NTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTD 884
Cdd:cd05920   315 SpDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGF-------YRTGDLVRRTPDGYLVVEGRIK 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  885 HQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPvAPDA--LRTEL-SRTLPDYMIPAVIVPV 961
Cdd:cd05920   388 DQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGERSCAFVVLRDP-PPSAaqLRRFLrERGLAAYKLPDRIEFV 466

                         490
                  ....*....|....*.
gi 653678460  962 PDFPTTPNGKLDRAAL 977
Cdd:cd05920   467 DSLPLTAVGKIDKKAL 482
EntF2 COG3319
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ...
 1539-2424 9.57e-46

Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442548 [Multi-domain]  Cd Length: 855  Bit Score: 180.28  E-value: 9.57e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1539 AQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDP 1618
Cdd:COG3319     1 AAAAAAAAAAAAAAAAAAAAAAAAAALAAAAAAAAAAALLLLAAALLVALAALALAALALAALLAVALLAAALALAALAA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1619 GYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQP 1698
Cdd:COG3319    81 LAALALALAAAAAALLLAALALLLALLAALALALLALLLAALLLALAALAAAAAAAALAAAAAAAAALAAAAGLGGGGGG 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1699 KGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADLAEELSGPLAYDYIR 1778
Cdd:COG3319   161 AGVLVLVLAALLALLLAALLALALALAALLLLALAAALALALLLLLALLLLLLLLLALLLLLLLALLAAAALLALLLALL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1779 LTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVkqLLELRPDAEVINHYGPTETVIGRVVHPVREAGELAVDSPLP 1858
Cdd:COG3319   241 LLLLAALLLLLALALLLLLALLLLLGLLALLLALLLL--LALLLLAAAAALAAGGTATTAAVTTTAAAAAPGVAGALGPI 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRGDGLLDFV 1938
Cdd:COG3319   319 GGGPGLLVLLVLLVLLLPLLLGVGGGGGGGGGGGGAGGLAGRGLRAAAALRDPAGAGARGRLRRGGDRGRRLGGGLLLGL 398

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALD 2018
Cdd:COG3319   399 GRLRLQRLRRGLREELEEAEAALAEAAAVAAAVAAAAAAAAAAAALAAAVVAAAALAAAALLLLLLLLLLPPPLPPALLL 478

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2019 AFPLTPHGKVNHRALPGPTSGRPELEDRYQAPRTPGEQLLAELWSTVLGTDRIGIEDNFFDLGGTSISVIQLSGACRRAG 2098
Cdd:COG3319   479 LLLLLLLLLLAALLLAAAAPAAAAAAAAAPAPAAALELALALLLLLLLGLGLVGDDDDFFGGGGGSLLALLLLLLLLALL 558

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2099 VEISPKDVFEQPTVRGQAMRAIARSRDDAYAPVLdtvfgaghtgttrtvVTLRGEGDGRPVFCVHPSGGGVAWYLPLARA 2178
Cdd:COG3319   559 LRLLLLLALLLAPTLAALAAALAAAAAAAALSPL---------------VPLRAGGSGPPLFCVHPAGGNVLCYRPLARA 623

                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2179 LPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGTIAFEMARQLDRLGEPV-QLILLEPT 2257
Cdd:COG3319   624 LGPDRPVYGLQAPGLDGGEPPPASVEEMAARYVEAIRAVQPEGPYHLLGWSFGGLVAYEMARQLEAQGEEVaLLVLLDSY 703

                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2258 LPEVARTIDLHRPAR---DSYLRGADLAERILRLPAGDPEgdRLRAELTRLLEDAGYSAGEISLGDALPMRACGQMLAAF 2334
Cdd:COG3319   704 APGALARLDEAELLAallRDLARGVDLPLDAEELRALDPE--ERLARLLERLREAGLPAGLDAERLRRLLRVFRANLRAL 781

                         810       820       830       840       850       860       870       880
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2335 AEYRPLPLPdeskVRTQFVVSAECTDATPERPsgtshtaygsyAAGWQKLLgrAAAPRTIHLPGIHATMLTTDRCPTVVS 2414
Cdd:COG3319   782 RRYRPRPYD----GPVLLFRAEEDPPGRADDP-----------ALGWRPLV--AGGLEVHDVPGDHFSMLREPHVAELAA 844

                         890
                  ....*....|
gi 653678460 2415 ACVEALRTTD 2424
Cdd:COG3319   845 ALRAALAAAE 854
DCL_NRPS-like cd19536
DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal ...
 48-459 1.30e-45

DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal fungal CT domains and Dual Epimerization/Condensation (E/C) domains; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type [D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L))], which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380459 [Multi-domain]  Cd Length: 419  Bit Score: 171.48  E-value: 1.30e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPVI 127
Cdd:cd19536     3 PLSSLQEGMLFHSLLNPGGSVYLHNYTYTVGRRLNLDLLLEALQVLIDRHDILRTSFIEDGLGQPVQVVHRQAQVPVTEL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  128 DVT---EADLPQALRTAAEQ--PFDLERGPLLRACLHRIGAKQHVLLV-TVHHIVLDGWSCGLLLADLARAYAGeLPAGV 201
Cdd:cd19536    83 DLTpleEQLDPLRAYKEETKirRFDLGRAPLVRAALVRKDERERFLLViSDHHSILDGWSLYLLVKEILAVYNQ-LLEYK 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  202 A-----APAFRDVAAWqhgRLAAGELDDQLRYWRQRLAG--LPDLEIPGD-RQRPADRDWrahSVRRELPAAteAAVRRL 273
Cdd:cd19536   162 PlslppAQPYRDFVAH---ERASIQQAASERYWREYLAGatLATLPALSEaVGGGPEQDS---ELLVSVPLP--VRSRSL 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  274 AAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR--GRTDLESVVGLFAGMVPVRLDLTGRpSFDEVLSRTVAGSR 351
Cdd:cd19536   234 AKRSGIPLSTLLLAAWALVLSRHSGSDDVVFGTVVHGRseETTGAERLLGLFLNTLPLRVTLSEE-TVEDLLKRAQEQEL 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  352 NDFAHPQIPfdrlVRELRRDriAGSQLLVQAMAGTESTAVALTFGEAAGTEQPVDVAL-----AKCDLDLSVLDDGDRLA 426
Cdd:cd19536   313 ESLSHEQVP----LADIQRC--SEGEPLFDSIVNFRHFDLDFGLPEWGSDEGMRRGLLfsefkSNYDVNLSVLPKQDRLE 386

                         410       420       430
                  ....*....|....*....|....*....|...
gi 653678460  427 VTLVAAADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19536   387 LKLAYNSQVLDEEQAQRLAAYYKSAIAELATAP 419
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
 1594-2033 1.33e-44

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 169.57  E-value: 1.33e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1594 DRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTvtpalvltrtgqggclpgAEVFGDVpvvaldrVADRLTAMPD 1673
Cdd:cd17654    49 DRGTESPVAILAILFLGAAYAPIDPASPEQRSLTVMKK------------------CHVSYLL-------QNKELDNAPL 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1674 QRPEVTVD-----PRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVS 1748
Cdd:cd17654   104 SFTPEHRHfnirtDECLAYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLSLSS 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1749 G-------RGVTLMPAdaTLADLAEELSGPlayDYIRLTPSHLRHLVGHWTGQELPPAA---RGWVVGGE-----TLDPA 1813
Cdd:cd17654   184 GatllivpTSVKVLPS--KLADILFKRHRI---TVLQATPTLFRRFGSQSIKSTVLSATsslRVLALGGEpfpslVILSS 258

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1814 LVKQLLELRpdaeVINHYGPTETVIGRVVHPVREAgelavDSPLPLGRPLGETRLQVLDAWLEAVPvgavGELFIGGdgI 1893
Cdd:cd17654   259 WRGKGNRTR----IFNIYGITEVSCWALAYKVPEE-----DSPVQLGSPLLGTVIEVRDQNGSEGT----GQVFLGG--L 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1894 ARGYlgrpaltaekFLPDPAGEPGARMYRTGDLVRwRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL 1973
Cdd:cd17654   324 NRVC----------ILDDEVTVPKGTMRATGDFVT-VKDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGVESCAVT 392

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1974 VRDQQLVGWFIPAEDHPPVTVSALRRFcaeQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17654   393 LSDQQRLIAFIVGESSSSRIHKELQLT---LLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
C_PKS-NRPS cd20483
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1082-1497 4.11e-44

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Most members of this subfamily have the typical C-domain HHXXXD motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380471 [Multi-domain]  Cd Length: 430  Bit Score: 167.44  E-value: 4.11e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAGggAAL 1161
Cdd:cd20483     1 PRPMSTFQRRLWFLHNFLEDKTFLNLLLVCHIKGKPDVNLLQKALSELVRRHEVLRTAYFEGDDFGEQQVLDDPS--FHL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPGTDLAQLLLAEAalgfTLATEHPL--------RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAA 1233
Cdd:cd20483    79 IVIDLSEAADPEAALDQLV----RNLRRQELdieegeviRGWLVKLPDEEFALVLASHHIAWDRGSSKSIFEQFTALYDA 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1234 DTGHREP-ELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAvaTEVAGDLP-----RPATPGGGGGAVEFALAP 1307
Cdd:cd20483   155 LRAGRDLaTVPPPPVQYIDFTLWHNALLQSPLVQPLLDFWKEKLEGI--PDASKLLPfakaeRPPVKDYERSTVEATLDK 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1308 RQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLR 1387
Cdd:cd20483   233 ELLARMKRICAQHAVTPFMFLLAAFRAFLYRYTEDEDLTIGMVDGDRPHPDFDDLVGFFVNMLPIRCRMDCDMSFDDLLE 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1388 RTHARTADALAHQELPFEHLV----ADRGAGNGPLFRIMYSFSSQEPAGRSAG------DVDLEPVPvpvTTCKFDL-VL 1456
Cdd:cd20483   313 STKTTCLEAYEHSAVPFDYIVdaldVPRSTSHFPIGQIAVNYQVHGKFPEYDTgdfkftDYDHYDIP---TACDIALeAE 389

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|.
gi 653678460 1457 TVVDGGstLEGVLEYADDLYLPGTAERLVTSLTNLLAAAVR 1497
Cdd:cd20483   390 EDPDGG--LDLRLEFSTTLYDSADMERFLDNFVTFLTSVIR 428
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
 517-977 1.26e-43

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 165.97  E-value: 1.26e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  597 atadrlgpDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRV 676
Cdd:cd05972    81 --------EDP---------------------------ALIYFTSGTTGLPKGVLHTHSYPLGHIPTAAYWLGLRPDDIH 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  677 LAT------TTVSFDIAGlelylPLRSGAGVVLADAD--TARNpaaLIDLAGRHRVTLAQATPTLWQALV-PELSGPALA 747
Cdd:cd05972   126 WNIadpgwaKGAWSSFFG-----PWLLGATVFVYEGPrfDAER---ILELLERYGVTSFCGPPTAYRMLIkQDLSSYKFS 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  748 GIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETTIwsTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVL 825
Cdd:cd05972   198 HLRLVVsAGEPLNPEVIEWWRAAtGLPIRDGYGQTETGL--TVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGRELPPGEE 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  826 GEL--HIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTL 903
Cdd:cd05972   276 GDIaiKLPPPGLFLGYVGDPEKTEASIRGD--------YYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESAL 347

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  904 INAGPVRRAAAVVREDrPGDLRLV-AYVI-PDGPVAPDALRTEL-----SRTLPdYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:cd05972   348 LEHPAVAEAAVVGSPD-PVRGEVVkAFVVlTSGYEPSEELAEELqghvkKVLAP-YKYPREIEFVEELPKTISGKIRRVE 425


                  .
gi 653678460  977 L 977
Cdd:cd05972   426 L 426
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
 635-977 1.34e-43

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 162.89  E-value: 1.34e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVsFDIAGLE-LYLPLRSGAGVVLADadtaRNPA 713
Cdd:cd17630     3 ATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPL-YHVGGLAiLVRSLLAGAELVLLE----RNQA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  714 ALIDLAgRHRVTLAQATPT-LWQALVPELSGPALAGIR-VLVGGEALPAELARRLGDAGAEVTNMYGPTETTiwSTSGPT 791
Cdd:cd17630    78 LAEDLA-PPGVTHVSLVPTqLQRLLDSGQGPAALKSLRaVLLGGAPIPPELLERAADRGIPLYTTYGMTETA--SQVATK 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 SEDSIRRGSIGVPIDNTQVYVLDAnlhpapigvlGELHIAGEGLARGYWNRPgltaekfLPDPFGPPGTrmYRTGDLVRW 871
Cdd:cd17630   155 RPDGFGRGGVGVLLPGRELRIVED----------GEIWVGGASLAMGYLRGQ-------LVPEFNEDGW--FTTKDLGEL 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  872 SAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPD 951
Cdd:cd17630   216 HADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPAELRAWLKDKLAR 295

                         330       340
                  ....*....|....*....|....*.
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd17630   296 FKLPKRIYPVPELPRTGGGKVDRRAL 321
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
 510-974 5.76e-43

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 164.92  E-value: 5.76e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  510 VIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP 589
Cdd:cd05914     1 LYYGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  590 VLV-VTDaatadrlgPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERF 668
Cdd:cd05914    81 KAIfVSD--------EDDV---------------------------ALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEVV 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  669 PLTAEDRVLATTTVSfDIAGL--ELYLPLRSGAGVVLADadtaRNPAALIDLAGRHRVTLAQATPTLWQ-------ALVP 739
Cdd:cd05914   126 LLGKGDKILSILPLH-HIYPLtfTLLLPLLNGAHVVFLD----KIPSAKIIALAFAQVTPTLGVPVPLViekifkmDIIP 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  740 ELSGP-------------------------ALAG-IRVLV-GGEALPAELARRLGDAGAEVTNMYGPTETT-IWSTSGPt 791
Cdd:cd05914   201 KLTLKkfkfklakkinnrkirklafkkvheAFGGnIKEFViGGAKINPDVEEFLRTIGFPYTIGYGMTETApIISYSPP- 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 seDSIRRGSIGVPIDNTQVYVLDanlhPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRW 871
Cdd:cd05914   280 --NRIRLGSAGKVIDGVEVRIDS----PDPATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDGW-------FHTGDLGKI 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  872 SAAGDLEYLGRTDHQVKL-RGFRIELGEIETTLINAGPVRRAAAVVREDRpgdLRLVAYVIPDGPVAP------------ 938
Cdd:cd05914   347 DAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNMPFVLESLVVVQEKK---LVALAYIDPDFLDVKalkqrniidaik 423

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 653678460  939 DALRTELSRTLPDY-MIPAVIVPVPDFPTTPNGKLDR 974
Cdd:cd05914   424 WEVRDKVNQKVPNYkKISKVKIVKEEFEKTPKGKIKR 460
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
1532-2033 1.21e-42

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 166.44  E-value: 1.21e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALR----TGD-RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAV 1606
Cdd:COG0365    10 IAYNCLDRHAEGRGDKVALIwegeDGEeRTLTYAELRREVNRFANALR--ALG---VKKGDRVAIYLPNIPEAVIAMLAC 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1607 LKAGGYFIPVDPGY-PMARLTRIADTvTPALVLTRTGQ---GGCLPGAEVFGDVP--------VVALDRV---------- 1664
Cdd:COG0365    85 ARIGAVHSPVFPGFgAEALADRIEDA-EAKVLITADGGlrgGKVIDLKEKVDEALeelpslehVIVVGRTgadvpmegdl 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1665 --ADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGV-------AVEHIGIVRY---------------LSWA-AASY 1719
Cdd:COG0365   164 dwDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVvhthggyLVHAATTAKYvldlkpgdvfwctadIGWAtGHSY 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1720 PTAGrrgTLAH--SSVGFD-----LTMSALFEpLVSGRGVTLMPADATL--ADLAEELSGPLAYDyirltPSHLRHLVGh 1790
Cdd:COG0365   244 IVYG---PLLNgaTVVLYEgrpdfPDPGRLWE-LIEKYGVTVFFTAPTAirALMKAGDEPLKKYD-----LSSLRLLGS- 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1791 wTGQELPPAARGWV---VGgetldpalvkqllelrpdAEVINHYGPTET----VIGRVVHPVReAGELavdsplplGRPL 1863
Cdd:COG0365   314 -AGEPLNPEVWEWWyeaVG------------------VPIVDGWGQTETggifISNLPGLPVK-PGSM--------GKPV 365

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1864 GETRLQVLDAWLEAVPVGAVGELFIGGD--GIARGYLGRPALTAEKFLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRV 1941
Cdd:COG0365   366 PGYDVAVVDEDGNPVPPGEEGELVIKGPwpGMFRGYWNDPERYRETYFGRFPG-----WYRTGDGARRDEDGYFWILGRS 440

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1942 DDQVKLRGYRIELGEIEARMAEHPGV-EQAVV----LVRDQQLVGWFIPAEDHPP--VTVSALRRFCAEQLPEFMVPNQW 2014
Cdd:COG0365   441 DDVINVSGHRIGTAEIESALVSHPAVaEAAVVgvpdEIRGQVVKAFVVLKPGVEPsdELAKELQAHVREELGPYAYPREI 520

                         570
                  ....*....|....*....
gi 653678460 2015 VALDAFPLTPHGKVNHRAL 2033
Cdd:COG0365   521 EFVDELPKTRSGKIMRRLL 539
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
 506-977 3.87e-42

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 163.47  E-value: 3.87e-42
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   506 GAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLS 585
Cdd:TIGR02262   20 GKTAFIDDISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLE 99

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   586 DARP-VLVVTDA---ATADRLG--PDDITGLVV---------LEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGV 650
Cdd:TIGR02262  100 DSRArVVFVSGAllpVIKAALGksPHLEHRVVVgrpeagevqLAELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGV 179

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   651 VITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGL--ELYLPLRSGAGVVL-ADADTarnPAALIDLAGRHRVTLA 727
Cdd:TIGR02262  180 VHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAYGLgnALTFPMSVGATTVLmGERPT---PDAVFDRLRRHQPTIF 256

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   728 QATPTLWQALVPELSGPALAGIRVLV---GGEALPAELARRLGDA-GAEVTNMYGPTETT-IWSTSGPtseDSIRRGSIG 802
Cdd:TIGR02262  257 YGVPTLYAAMLADPNLPSEDQVRLRLctsAGEALPAEVGQRWQARfGVDIVDGIGSTEMLhIFLSNLP---GDVRYGTSG 333

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   803 VPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLpdpfGPpgtrMYRTGDLVRWSAAGDLEYLGR 882
Cdd:TIGR02262  334 KPVPGYRLRLVGDGGQDVADGEPGELLISGPSSATMYWNNRAKSRDTFQ----GE----WTRSGDKYVRNDDGSYTYAGR 405

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   883 TDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVP 960
Cdd:TIGR02262  406 TDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIKPKAFVVlrPGQTALETELKEHVKDRLAPYKYPRWIVF 485

                          490
                   ....*....|....*..
gi 653678460   961 VPDFPTTPNGKLDRAAL 977
Cdd:TIGR02262  486 VDDLPKTATGKIQRFKL 502
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 518-977 1.89e-39

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 154.13  E-value: 1.89e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  518 SYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDaa 597
Cdd:cd05971     8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTD-- 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  598 tadrlGPDDitglvvleetdtggypateppavpaghSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVL 677
Cdd:cd05971    86 -----GSDD---------------------------PALIIYTSGTTGPPKGALHAHRVLLGHLPGVQFPFNLFPRDGDL 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  678 ATTTVSFD-IAGL-ELYLPLRSGAGVVLADADTARNPAALIDLAGRHRVTLAQATPT---LWQALVPELSGPALAGIRVL 752
Cdd:cd05971   134 YWTPADWAwIGGLlDVLLPSLYFGVPVLAHRMTKFDPKAALDLMSRYGVTTAFLPPTalkMMRQQGEQLKHAQVKLRAIA 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  753 VGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPtSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELhia 831
Cdd:cd05971   214 TGGESLGEELLGWAREQfGVEVNEFYGQTECNLVIGNCS-ALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEI--- 289

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 geGLAR-------GYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLI 904
Cdd:cd05971   290 --AVELpdpvaflGYWNNPSATEKKMAGDWL--------LTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLL 359

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460  905 NAGPVRRAAAVVREDRPGDLRLVAYVI-PDGPVAPDALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05971   360 KHPAVLMAAVVGIPDPIRGEIVKAFVVlNPGETPSDALAREIqelvKTRLAAHEYPREIEFVNELPRTATGKIRRREL 437
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
 512-972 1.89e-39

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 155.06  E-value: 1.89e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  512 EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVL 591
Cdd:cd05911     6 DTGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKV 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  592 VVTDA-------ATADRLGPDD----ITGLV--------VLEETDTGGYPA-TEPPAVPAGHSAYVIYTSGSTGRPKGVV 651
Cdd:cd05911    86 IFTDPdglekvkEAAKELGPKDkiivLDDKPdgvlsiedLLSPTLGEEDEDlPPPLKDGKDDTAAILYSSGTTGLPKGVC 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAAL--DNFLAAMAERFPLTAEDRVLATTTVsFDIAGLE--LYLPLRSGAGVVLADADtarnPAALIDLAGRHRVTLA 727
Cdd:cd05911   166 LSHRNLiaNLSQVQTFLYGNDGSNDVILGFLPL-YHIYGLFttLASLLNGATVIIMPKFD----SELFLDLIEKYKITFL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  728 QATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLG--DAGAEVTNMYGPTETTiwSTSGPTSEDSIRRGSIG 802
Cdd:cd05911   241 YLVPPIAAALAksPLLDKYDLSSLRvILSGGAPLSKELQELLAkrFPNATIKQGYGMTETG--GILTVNPDGDDKPGSVG 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  803 VPIDNTQVYVLDANLHPA-PIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLG 881
Cdd:cd05911   319 RLLPNVEAKIVDDDGKDSlGPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGW-------LHTGDIGYFDEDGYLYIVD 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  882 RTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPdgpvAPDALRTELSrtLPDYMIPAV---- 957
Cdd:cd05911   392 RKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVR----KPGEKLTEKE--VKDYVAKKVasyk 465

                         490       500
                  ....*....|....*....|
gi 653678460  958 -----IVPVPDFPTTPNGKL 972
Cdd:cd05911   466 qlrggVVFVDEIPKSASGKI 485
C_PKS-NRPS_PksJ-like cd20484
Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS ...
 1084-1499 2.14e-39

Condensation domain of hybrid polyketide synthetase/nonribosomal peptide synthetases (PKS/NRPSs), similar to Bacillus subtilis PksJ; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Hybrid PKS/NRPS create polymers containing both polyketide and amide linkages. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily have the typical C-domain HHxxxD motif. PksJ is involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is important in secondary metabolism. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380472 [Multi-domain]  Cd Length: 430  Bit Score: 153.63  E-value: 2.14e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPgPDGMPVQVADPAGGgAALTE 1163
Cdd:cd20484     3 PLSEGQKGLWMLQKMSPEMSAYNVPLCFRFSSKLDVEKFKQACQFVLEQHPILKSVIEE-EDGVPFQKIEPSKP-LSFQE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1164 RD--AAPGTDLAQLLLAEAALGFTLAtEHPL-RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHREP 1240
Cdd:cd20484    81 EDisSLKESEIIAYLREKAKEPFVLE-NGPLmRVHLFSRSEQEHFVLITIHHIIFDGSSSLTLIHSLLDAYQALLQGKQP 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1241 ELDEPALAQADYAVWQRQ--SAQRGryAAELDFWRTELTGAVAT-EVAGDLPRPATPGGGGGAVEFALAPRQIQGIRELA 1317
Cdd:cd20484   160 TLASSPASYYDFVAWEQDmlAGAEG--EEHRAYWKQQLSGTLPIlELPADRPRSSAPSFEGQTYTRRLPSELSNQIKSFA 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1318 RRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADAL 1397
Cdd:cd20484   238 RSQSINLSTVFLGIFKLLLHRYTGQEDIIVGMPTMGRPEERFDSLIGYFINMLPIRSRILGEETFSDFIRKLQLTVLDGL 317

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1398 AHQELPFEHLVAD----RGAGNGPLFRIMYSF-------SSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTLE 1466
Cdd:cd20484   318 DHAAYPFPAMVRDlnipRSQANSPVFQVAFFYqnflqstSLQQFLAEYQDVLSIEFVEGIHQEGEYELVLEVYEQEDRFT 397

                         410       420       430
                  ....*....|....*....|....*....|...
gi 653678460 1467 GVLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd20484   398 LNIKYNPDLFDASTIERMMEHYVKLAEELIANP 430
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
 505-931 4.62e-39

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 154.31  E-value: 4.62e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDT--TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAY 582
Cdd:cd05904    19 PSRPALIDAATgrALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAK 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  583 LLSDARPVLVVTDAATADRLgPDDITGLVVLEETDTGG---------YPATEPPAVPAGHS--AYVIYTSGSTGRPKGVV 651
Cdd:cd05904    99 QVKDSGAKLAFTTAELAEKL-ASLALPVVLLDSAEFDSlsfsdllfeADEAEPPVVVIKQDdvAALLYSSGTTGRSKGVM 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAaldNFLAAMA-----ERFPLTAEDRVLATTTVsFDIAGLELYL--PLRSGAG-VVLADADTarnpAALIDLAGRHR 723
Cdd:cd05904   178 LTHR---NLIAMVAqfvagEGSNSDSEDVFLCVLPM-FHIYGLSSFAlgLLRLGATvVVMPRFDL----EELLAAIERYK 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  724 VTLAQATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGPTSE-DSIR 797
Cdd:cd05904   250 VTHLPVVPPIVLALVksPIVDKYDLSSLRqIMSGAAPLGKELIEAFRAKfpNVDLGQGYGMTESTGVVAMCFAPEkDRAK 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  798 RGSIGVPIDNTQVYVLDAN-LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGD 876
Cdd:cd05904   330 YGSVGRLVPNVEAKIVDPEtGESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEGW-------LHTGDLCYIDEDGY 402

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  877 LEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI 931
Cdd:cd05904   403 LFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVV 457
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
 1541-2028 4.77e-39

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 152.76  E-value: 4.77e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPgy 1620
Cdd:cd17631     5 ARRHPDRTALVFGGRSLTYAELDERVNRLAHALR--ALG---VAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNF-- 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 pmaRLTRiadtvtpalvltrtgqggclpgaevfgdvpvvalDRVADRLTampDQRPEVTVDprGLAYSIFTSGSTGQPKG 1700
Cdd:cd17631    78 ---RLTP----------------------------------PEVAYILA---DSGAKVLFD--DLALLMYTSGTTGRPKG 115

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1701 VAVEHigivRYLSWAAASYPTA---GRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMP-----ADATLADLAEElsgpl 1772
Cdd:cd17631   116 AMLTH----RNLLWNAVNALAAldlGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVIlrkfdPETVLDLIERH----- 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 AYDYIRLTPSHLRHLVGH--WTGQELPpAARGWVVGGETLDPALVKQLLELrpDAEVINHYGPTETVIGRVVHPvreaGE 1850
Cdd:cd17631   187 RVTSFFLVPTMIQALLQHprFATTDLS-SLRAVIYGGAPMPERLLRALQAR--GVKFVQGYGMTETSPGVTFLS----PE 259

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1851 LAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepgarMYRTGDLVRWR 1930
Cdd:cd17631   260 DHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRDG--------WFHTGDLGRLD 331

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1931 GDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQlvgW------FIPAEDHPPVTVSALRRFCAE 2003
Cdd:cd17631   332 EDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVaEVAVIGVPDEK---WgeavvaVVVPRPGAELDEDELIAHCRE 408

                         490       500
                  ....*....|....*....|....*
gi 653678460 2004 QLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd17631   409 RLARYKIPKSVEFVDALPRNATGKI 433
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 512-977 6.25e-39

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 154.33  E-value: 6.25e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  512 EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVL 591
Cdd:cd12119    21 GEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRV 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  592 VVTDAATADRLGP-----DDITGLVVLeeTDTGGYPATEPPAVPA------------------GHSAYVI-YTSGSTGRP 647
Cdd:cd12119   101 VFVDRDFLPLLEAiaprlPTVEHVVVM--TDDAAMPEPAGVGVLAyeellaaespeydwpdfdENTAAAIcYTSGTTGNP 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  648 KGVVITRAALdnFLAAMAER----FPLTAEDRVLATTTVsFDIAGLEL-YLPLRSGAGVVLADADTarNPAALIDLAGRH 722
Cdd:cd12119   179 KGVVYSHRSL--VLHAMAALltdgLGLSESDVVLPVVPM-FHVNAWGLpYAAAMVGAKLVLPGPYL--DPASLAELIERE 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLWQALVPELSGPA---LAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTET-TIWSTSGPTS------ 792
Cdd:cd12119   254 GVTFAAGVPTVWQGLLDHLEANGrdlSSLRRVVIGGSAVPRSLIEAFEERGVRVIHAWGMTETsPLGTVARPPSehsnls 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  793 --EDSIRRGSIGVPIDNTQVYVLDANLHPAPI--GVLGELHIAGEGLARGYWNRPGlTAEKFLPDPFgppgtrmYRTGDL 868
Cdd:cd12119   334 edEQLALRAKQGRPVPGVELRIVDDDGRELPWdgKAVGELQVRGPWVTKSYYKNDE-ESEALTEDGW-------LRTGDV 405

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  869 VRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELS 946
Cdd:cd12119   406 ATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVlkEGATVTAEELLEFLA 485

                         490       500       510
                  ....*....|....*....|....*....|.
gi 653678460  947 RTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd12119   486 DKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
PRK06188 PRK06188
acyl-CoA synthetase; Validated
 502-988 8.63e-39

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 153.99  E-value: 8.63e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PRK06188   23 KRYPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRTALHPLGSLDDHA 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARP-VLVVTDAATADR----------------LGPDDiTGLVVLEETDTGGYPATEPPAVPAgHSAYVIYTSGST 644
Cdd:PRK06188  103 YVLEDAGIsTLIVDPAPFVERalallarvpslkhvltLGPVP-DGVDLLAAAAKFGPAPLVAAALPP-DIAGLAYTGGTT 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  645 GRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFdiAGLELYLP--LRSGAGVVLADADtarnPAALIDLAGRH 722
Cdd:PRK06188  181 GKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSH--AGGAFFLPtlLRGGTVIVLAKFD----PAEVLRAIEEQ 254

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLWQALV--PELSGPALAGIRVLVGGEA--LPAELA---RRLGDAGAEVtnmYGPTETTIWSTSGPTSE-- 793
Cdd:PRK06188  255 RITATFLVPTMIYALLdhPDLRTRDLSSLETVYYGASpmSPVRLAeaiERFGPIFAQY---YGQTEAPMVITYLRKRDhd 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  794 --DSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRW 871
Cdd:PRK06188  332 pdDPKRLTSCGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAFRDG--------WLHTGDVARE 403

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  872 SAAGDLEYLGRTDHQVKLRGFRIELGEIETTLiNAGPVRRAAAV--VREDRPGDLrLVAYVI--PDGPVAPDALRTELSR 947
Cdd:PRK06188  404 DEDGFYYIVDRKKDMIVTGGFNVFPREVEDVL-AEHPAVAQVAVigVPDEKWGEA-VTAVVVlrPGAAVDAAELQAHVKE 481

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 653678460  948 TLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTRSTA 988
Cdd:PRK06188  482 RKGSVHAPKQVDFVDSLPLTALGKPDKKALRARYWEGRGRA 522
C_NRPS-like cd19537
Condensation family domain with an atypical active site motif; Condensation (C) domains of ...
 57-368 8.73e-39

Condensation family domain with an atypical active site motif; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily typically have a non-canonical conserved SHXXXDX(14)Y motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380460 [Multi-domain]  Cd Length: 395  Bit Score: 150.80  E-value: 8.73e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   57 WFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGpDGEPVQQTAPAAPVALPVIDVteadlpq 136
Cdd:cd19537    12 WHKYQLSTGTSSFNVSFACRLSGDVDRDRLASAWNTVLARHRILRSRYVPR-DGGLRRSYSSSPPRVQRVDTL------- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  137 ALRTAAEQPFDLERGPLLRACLhrigaKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGELPAGVAAPAFrDVAAWQHgR 216
Cdd:cd19537    84 DVWKEINRPFDLEREDPIRVFI-----SPDTLLVVMSHIICDLTTLQLLLREVSAAYNGKLLPPVRREYL-DSTAWSR-P 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  217 LAAGELDdqlrYWRQRLAGLPDLEIPGdrqRPADRDWRAHSVRRELPAATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRL 296
Cdd:cd19537   157 ASPEDLD----FWSEYLSGLPLLNLPR---RTSSKSYRGTSRVFQLPGSLYRSLLQFSTSSGITLHQLALAAVALALQDL 229

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  297 TGQDDFAVGVPVAGRGRTDLESVVGLFAGMVPVRL--DLTGRPSFDEVLSRTVAGSRNDFAHPqIPFDRLVREL 368
Cdd:cd19537   230 SDRTDIVLGAPYLNRTSEEDMETVGLFLEPLPIRIrfPSSSDASAADFLRAVRRSSQAALAHA-IPWHQLLEHL 302
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
 489-972 2.19e-38

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 153.01  E-value: 2.19e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  489 PAASLRERFQQWCRR----TPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKA 564
Cdd:PRK07786   11 PYLARRQNWVNQLARhalmQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANML 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  565 GAAYLPIDPDFPVERIAYLLSDARPVLVVTD------AATADRLGPDDITGLVVLEETDTG--GY--------PATEPPA 628
Cdd:PRK07786   91 GAIAVPVNFRLTPPEIAFLVSDCGAHVVVTEaalapvATAVRDIVPLLSTVVVAGGSSDDSvlGYedllaeagPAHAPVD 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  629 VPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLP-LRSGAGVVLADAD 707
Cdd:PRK07786  171 IPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADINSDVGFVGVPLFHIAGIGSMLPgLLLGAPTVIYPLG 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  708 tARNPAALIDLAGRHRVTLAQATPTLWQAL--VPELSGPALAgIRVLVGGEAlPAE--LARRLGDA--GAEVTNMYGPTE 781
Cdd:PRK07786  251 -AFDPGQLLDVLEAEKVTGIFLVPAQWQAVcaEQQARPRDLA-LRVLSWGAA-PASdtLLRQMAATfpEAQILAAFGQTE 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  782 TTIwSTSGPTSEDSIR-RGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgt 860
Cdd:PRK07786  328 MSP-VTCMLLGEDAIRkLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAFAGGWF----- 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  861 rmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAP-- 938
Cdd:PRK07786  402 ---HSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDDAAlt 478

                         490       500       510
                  ....*....|....*....|....*....|....*
gi 653678460  939 -DALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK07786  479 lEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKV 513
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
 1531-2033 2.20e-38

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 152.37  E-value: 2.20e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1531 RTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAG 1610
Cdd:PRK07656    5 MTLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALA--ALG---IGKGDRVAIWAPNSPHWVIAALGALKAG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1611 GYFIPVDPGYPMARLTRI-ADTVTPALVltrtGQGGCLPGAEVfGDVPVVALDRVA-----------------DRLTAMP 1672
Cdd:PRK07656   80 AVVVPLNTRYTADEAAYIlARGDAKALF----VLGLFLGVDYS-ATTRLPALEHVViceteeddphtekmktfTDFLAAG 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1673 DQR-PEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVR-YLSWAAASYPTAGRRgTLA-----HSsvgFDLTmSALFEP 1745
Cdd:PRK07656  155 DPAeRAPEVDPDDVADILFTSGTTGRPKGAMLTHRQLLSnAADWAEYLGLTEGDR-YLAanpffHV---FGYK-AGVNAP 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1746 LVSGRGVTLMP---ADATLADLAEE----LSGPLA-----YDYIRLTPSHLRHLvghwtgqelppaaRGWVVGGETLDPA 1813
Cdd:PRK07656  230 LMRGATILPLPvfdPDEVFRLIETEritvLPGPPTmynslLQHPDRSAEDLSSL-------------RLAVTGAASMPVA 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1814 LVKQLLELRPDAEVINHYGPTE----TVIGRvvhpvreAGELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIG 1889
Cdd:PRK07656  297 LLERFESELGVDIVLTGYGLSEasgvTTFNR-------LDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVR 369

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1890 GDGIARGYLGRPALTAEKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ 1969
Cdd:PRK07656  370 GPNVMKGYYDDPEATAAAIDAD-------GWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAE 442

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1970 AVVL-VRDQQL---VGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK07656  443 AAVIgVPDERLgevGKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
 517-977 2.60e-38

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 150.32  E-value: 2.60e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:cd05935     2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  597 ATadrlgpDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRV 676
Cdd:cd05935    82 EL------DDL---------------------------ALIPYTSGTTGLPKGCMHTHFSAAANALQSAVWTGLTPSDVI 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  677 LATTTVsFDIAGLE--LYLPLRSGAGVVLA---DADTARnpaaliDLAGRHRVTLAQATPTLWQALV--PELSGPALAGI 749
Cdd:cd05935   129 LACLPL-FHVTGFVgsLNTAVYVGGTYVLMarwDRETAL------ELIEKYKVTFWTNIPTMLVDLLatPEFKTRDLSSL 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 RVLVGGEA-LPAELARRLGD-AGAEVTNMYGPTETTIWSTSGPTSedSIRRGSIGVPIDNTQVYVLDA-NLHPAPIGVLG 826
Cdd:cd05935   202 KVLTGGGApMPPAVAEKLLKlTGLRFVEGYGLTETMSQTHTNPPL--RPKLQCLGIP*FGVDARVIDIeTGRELPPNEVG 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  827 ELHIAGEGLARGYWNRPGLTAEKFLPDpfgpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINA 906
Cdd:cd05935   280 EIVVRGPQIFKGYWNRPEETEESFIEI----KGRRFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKH 355

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  907 GPVRRAAAV-VREDRPGDlRLVAYVIPD----GPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05935   356 PAI*EVCVIsVPDERVGE-EVKAFIVLRpeyrGKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
PRK06164 PRK06164
acyl-CoA synthetase; Validated
 490-967 4.00e-38

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 152.20  E-value: 4.00e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  490 AASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYL 569
Cdd:PRK06164    9 ADTLASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVI 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  570 PIDPDFPVERIAYLLSDARPVLV------------------------------VTDAATADRLGPDDITGLVVLEETDTG 619
Cdd:PRK06164   89 AVNTRYRSHEVAHILGRGRARWLvvwpgfkgidfaailaavppdalpplraiaVVDDAADATPAPAPGARVQLFALPDPA 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  620 GYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVS--FDIAGleLYLPLRS 697
Cdd:PRK06164  169 PPAAAGERAADPDAGALLFTTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCgvFGFST--LLGALAG 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  698 GAGVVLADA-DTARNPAALidlaGRHRVTLAQATPTLWQALVPELSGPA-LAGIRVLVGGEALPA--ELARRLGDAGAEV 773
Cdd:PRK06164  247 GAPLVCEPVfDAARTARAL----RRHRVTHTFGNDEMLRRILDTAGERAdFPSARLFGFASFAPAlgELAALARARGVPL 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  774 TNMYGPTETTIWSTSGPTSED-SIRRGSIGVPID-NTQVYVLD-ANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKF 850
Cdd:PRK06164  323 TGLYGSSEVQALVALQPATDPvSVRIEGGGRPASpEARVRARDpQDGALLPDGESGEIEIRAPSLMRGYLDNPDATARAL 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  851 LPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLiNAGPVRRAAAVVREDRPGDLRLVAYV 930
Cdd:PRK06164  403 TDDGY-------FRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHAL-EALPGVAAAQVVGATRDGKTVPVAFV 474

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 653678460  931 IPDGPVAPDA--LRTELSRTLPDYMIPAVIVPVPDFPTT 967
Cdd:PRK06164  475 IPTDGASPDEagLMAACREALAGFKVPARVQVVEAFPVT 513
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
 515-963 7.37e-38

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 149.67  E-value: 7.37e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVT 594
Cdd:cd05907     4 QPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 DaatadrlGPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITraaLDNFLA---AMAERFPLT 671
Cdd:cd05907    84 E-------DPDDL---------------------------ATIIYTSGTTGRPKGVMLS---HRNILSnalALAERLPAT 126

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  672 AEDRVLA---TTTVSFDIAGleLYLPLRSGAGVVLadadtARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPALAG 748
Cdd:cd05907   127 EGDRHLSflpLAHVFERRAG--LYVPLLAGARIYF-----ASSAETLLDDLSEVRPTVFLAVPRVWEKVYAAIKVKAVPG 199

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  749 IR--------------VLVGGEALPAELARRLGDAGAEVTNMYGPTETTiwSTSGPTSEDSIRRGSIGVPIDNTQVyvld 814
Cdd:cd05907   200 LKrklfdlavggrlrfAASGGAPLPAELLHFFRALGIPVYEGYGLTETS--AVVTLNPPGDNRIGTVGKPLPGVEV---- 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  815 anlhpaPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFR- 893
Cdd:cd05907   274 ------RIADDGEILVRGPNVMLGYYKNPEATAEALDADGW-------LHTGDLGEIDEDGFLHITGRKKDLIITSGGKn 340

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  894 IELGEIETTLINAGPVRRaAAVVREDRPgdlRLVAYVIPDgpvaPDALRTELSRTLPDYMIPAVIVPVPD 963
Cdd:cd05907   341 ISPEPIENALKASPLISQ-AVVIGDGRP---FLVALIVPD----PEALEAWAEEHGIAYTDVAELAANPA 402
PRK08316 PRK08316
acyl-CoA synthetase; Validated
 502-972 9.04e-38

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 150.85  E-value: 9.04e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PRK08316   22 RRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEELA 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARPVLVVTDAATADRL-------GPDDITGLVVLEETDTGG-----------YPATEPPAVPAGHS-AYVIYTSG 642
Cdd:PRK08316  102 YILDHSGARAFLVDPALAPTAeaalallPVDTLILSLVLGGREAPGgwldfadwaeaGSVAEPDVELADDDlAQILYTSG 181

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  643 STGRPKGVVIT-RAALDNFLAAMAErFPLTAEDRVLATTTVsFDIAGLELYL-P-LRSGAGVVLADADTarnPAALIDLA 719
Cdd:PRK08316  182 TESLPKGAMLThRALIAEYVSCIVA-GDMSADDIPLHALPL-YHCAQLDVFLgPyLYVGATNVILDAPD---PELILRTI 256

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  720 GRHRVTLAQATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTS-GPtsE 793
Cdd:PRK08316  257 EAERITSFFAPPTVWISLLrhPDFDTRDLSSLRkGYYGASIMPVEVLKELRERlpGLRFYNCYGQTEIAPLATVlGP--E 334

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  794 DSIRR-GSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWS 872
Cdd:PRK08316  335 EHLRRpGSAGRPVLNVETRVVDDDGNDVAPGEVGEIVHRSPQLMLGYWDDPEKTAEAFRGGWF--------HSGDLGVMD 406

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  873 AAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDG--PVAPDALRTELSRTLP 950
Cdd:PRK08316  407 EEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAgaTVTEDELIAHCRARLA 486

                         490       500
                  ....*....|....*....|..
gi 653678460  951 DYMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK08316  487 GFKVPKRVIFVDELPRNPSGKI 508
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
 1535-2033 1.45e-37

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 148.87  E-value: 1.45e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1535 QLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFI 1614
Cdd:cd05936     3 DLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQ--NLG---VQPGDRVALMLPNCPQFPIAYFGALKAGAVVV 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 PVDPGYPMARLTRIadtvtpalvLTRTGqggclpgAEVfgdvpVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGS 1694
Cdd:cd05936    78 PLNPLYTPRELEHI---------LNDSG-------AKA-----LIVAVSFTDLLAAGAPLGERVALTPEDVAVLQYTSGT 136

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGT-------LAHSsvgFDLTMsALFEPLVSGRGVTLMP---ADATLADL 1764
Cdd:cd05936   137 TGVPKGAMLTHRNLVANALQIKAWLEDLLEGDDvvlaalpLFHV---FGLTV-ALLLPLALGATIVLIPrfrPIGVLKEI 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1765 AEElsgplaydyiRLT-----PSHLRHLVGHWTGQELPPAA-RGWVVGGETLDPALVKQLLElRPDAEVINHYGPTETVI 1838
Cdd:cd05936   213 RKH----------RVTifpgvPTMYIALLNAPEFKKRDFSSlRLCISGGAPLPVEVAERFEE-LTGVPIVEGYGLTETSP 281

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1839 GRVVHPVREagelaVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepga 1918
Cdd:cd05936   282 VVAVNPLDG-----PRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFVDG------- 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1919 rMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQ----LVGWFIPAEDHpPVT 1993
Cdd:cd05936   350 -WLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVgVPDPYsgeaVKAFVVLKEGA-SLT 427

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 653678460 1994 VSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05936   428 EEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRREL 467
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
482-942 2.31e-37

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 151.02  E-value: 2.31e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  482 NDTARPLPAASLRERFQQWCRRTPGAVAVIEGD----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVT 557
Cdd:COG1022     2 SEFSDVPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  558 LLAVIKAGAAYLPIDPDFPVERIAYLLSDARP-VLVVTDAATADRLGP--DDITGL---VVLEETDTGGYP--------- 622
Cdd:COG1022    82 DLAILAAGAVTVPIYPTSSAEEVAYILNDSGAkVLFVEDQEQLDKLLEvrDELPSLrhiVVLDPRGLRDDPrllsldell 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  623 -----ATEPPAVPAGHS-------AYVIYTSGSTGRPKGVVITRAaldNFLA---AMAERFPLTAEDRVLatttvsfdia 687
Cdd:COG1022   162 algreVADPAELEARRAavkpddlATIIYTSGTTGRPKGVMLTHR---NLLSnarALLERLPLGPGDRTL---------- 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  688 gleLYLPL-----RSG------AGVVLAdadTARNPAALIDLAGRHRVTLAQATPTLW-------QALVPELSGP----- 744
Cdd:COG1022   229 ---SFLPLahvfeRTVsyyalaAGATVA---FAESPDTLAEDLREVKPTFMLAVPRVWekvyagiQAKAEEAGGLkrklf 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ----------------------------ALA------------G--IRVLV-GGEALPAELARRLGDAGAEVTNMYGPTE 781
Cdd:COG1022   303 rwalavgrryararlagkspslllrlkhALAdklvfsklrealGgrLRFAVsGGAALGPELARFFRALGIPVLEGYGLTE 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  782 ttiwsTSGPTS---EDSIRRGSIGVPIDNTQVYvldanlhpapIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgpp 858
Cdd:COG1022   383 -----TSPVITvnrPGDNRIGTVGPPLPGVEVK----------IAEDGEILVRGPNVMKGYYKNPEATAEAFDADGW--- 444

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  859 gtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLR-GFRIELGEIETTLinagpvrRA------AAVVREDRPgdlRLVAYVI 931
Cdd:COG1022   445 ----LHTGDIGELDEDGFLRITGRKKDLIVTSgGKNVAPQPIENAL-------KAsplieqAVVVGDGRP---FLAALIV 510

                         570
                  ....*....|.
gi 653678460  932 PDgpvaPDALR 942
Cdd:COG1022   511 PD----FEALG 517
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
 502-972 2.35e-37

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 150.81  E-value: 2.35e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVI-EGDT-----TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:cd17634    64 RENGDRTAIIyEGDDtsqsrTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGF 143

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  576 PVERIAYLLSDARPVLVVT---------------DAATADRLGPDDITGLVVLEETD-----TGGY------------PA 623
Cdd:cd17634   144 APEAVAGRIIDSSSRLLITadggvragrsvplkkNVDDALNPNVTSVEHVIVLKRTGsdidwQEGRdlwwrdliakasPE 223

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  624 TEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLA-AMAERFPLTAEDRVLATTTVSFDIAGLEL-YLPLRSGAGV 701
Cdd:cd17634   224 HQPEAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAAtTMKYVFDYGPGDIYWCTADVGWVTGHSYLlYGPLACGATT 303

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  702 VLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALVPE----LSGPALAGIRVLVG-GEALPAE----LARRLGDAGA 771
Cdd:cd17634   304 LLYEgVPNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAgddaIEGTDRSSLRILGSvGEPINPEayewYWKKIGKEKC 383

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  772 EVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE--GLARGYWNRPGLTAEK 849
Cdd:cd17634   384 PVVDTWWQTETGGFMITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPwpGQTRTLFGDHERFEQT 463

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  850 FLPDPFGppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAY 929
Cdd:cd17634   464 YFSTFKG-----MYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAY 538

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460  930 VI-----PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:cd17634   539 VVlnhgvEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
 499-976 3.57e-37

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 149.70  E-value: 3.57e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  499 QWCRRTPGAVAVI------EGDTTLSYRELDERANRLARLLMERGAGAETfVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:cd05931     1 RRAAARPDRPAYTflddegGREETLTYAELDRRARAIAARLQAVGKPGDR-VLLLAPPGLDFVAAFLGCLYAGAIAVPLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFP---VERIAYLLSDARPVLVVTDAATADRLGP-------DDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSG 642
Cdd:cd05931    80 PPTPgrhAERLAAILADAGPRVVLTTAAALAAVRAfaasrpaAGTPRLLVVDLLPDTSAADWPPPSPDPDDIAYLQYTSG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  643 STGRPKGVVIT-RAALDNfLAAMAERFPLTAEDRVlatttVSFdiagLELY----------LPLRSGAGVVL-ADADTAR 710
Cdd:cd05931   160 STGTPKGVVVThRNLLAN-VRQIRRAYGLDPGDVV-----VSW----LPLYhdmgliggllTPLYSGGPSVLmSPAAFLR 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  711 NPAALIDLAGRHRVTLAQAtPTLWQALV------PELSGPALAGIRV-LVGGEALPAELARRLGDAGAE-------VTNM 776
Cdd:cd05931   230 RPLRWLRLISRYRATISAA-PNFAYDLCvrrvrdEDLEGLDLSSWRVaLNGAEPVRPATLRRFAEAFAPfgfrpeaFRPS 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  777 YGPTETTIWSTSGPT------------------------SEDSIRRGSIGVPIDNTQVYVLDANLH-PAPIGVLGELHIA 831
Cdd:cd05931   309 YGLAEATLFVSGGPPgtgpvvlrvdrdalagravavaadDPAARELVSCGRPLPDQEVRIVDPETGrELPDGEVGEIWVR 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 GEGLARGYWNRPGLTAEKFLPDPfGPPGTRMYRTGDLvRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR 911
Cdd:cd05931   389 GPSVASGYWGRPEATAETFGALA-ATDEGGWLRTGDL-GFLHDGELYITGRLKDLIIVRGRNHYPQDIEATAEEAHPALR 466

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  912 ----AAAVVREDRPGDLRLVAYVIPD-GPVAPDALRTELSRTLPDY--MIPAVIVPVP--DFPTTPNGKLDRAA 976
Cdd:cd05931   467 pgcvAAFSVPDDGEERLVVVAEVERGaDPADLAAIAAAIRAAVAREhgVAPADVVLVRpgSIPRTSSGKIQRRA 540
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1554-2034 3.84e-37

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 146.67  E-value: 3.84e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1554 DRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVT 1633
Cdd:cd05934     1 GRRWTYAELLRESARIAAALA--ALG---IRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSG 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1634 PALVLTrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPrglAYSIFTSGSTGQPKGVAVEHigivRYLS 1713
Cdd:cd05934    76 AQLVVV-----------------------------------------DP---ASILYTSGTTGPPKGVVITH----ANLT 107

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1714 WAAASYPTAGRRGT---------LAHSSVGFDLTMSALfeplVSGRGVTLMP---ADATLADLAEelSGPLAYDYIRLTP 1781
Cdd:cd05934   108 FAGYYSARRFGLGEddvyltvlpLFHINAQAVSVLAAL----SVGATLVLLPrfsASRFWSDVRR--YGATVTNYLGAML 181

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1782 SHL----------RHLVGHWTGQELPPAargwvvggetldpalVKQLLELRPDAEVINHYGPTET---VIGRVVHPVRea 1848
Cdd:cd05934   182 SYLlaqppspddrAHRLRAAYGAPNPPE---------------LHEEFEERFGVRLLEGYGMTETivgVIGPRDEPRR-- 244

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1849 gelavdsPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFI---GGDGIARGYLGRPALTAEKFlpdPAGepgarMYRTGD 1925
Cdd:cd05934   245 -------PGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIrglRGWGFFKGYYNMPEATAEAM---RNG-----WFHTGD 309

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1926 LVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ---QLVGWFIPAEDHPPVTVSALRRFC 2001
Cdd:cd05934   310 LGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVrEAAVVAVPDEvgeDEVKAVVVLRPGETLDPEELFAFC 389

                         490       500       510
                  ....*....|....*....|....*....|...
gi 653678460 2002 AEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:cd05934   390 EGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
 507-977 4.82e-37

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 146.85  E-value: 4.82e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  507 AVAVIEGDTTLSYRELDERANRLARLLMerGAGAETF---VAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYL 583
Cdd:cd05958     1 RTCLRSPEREWTYRDLLALANRIANVLV--GELGIVPgnrVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDAATADRlgpDDITGLVvleetdtggypateppavpaghsayviYTSGSTGRPKGVVitrAALDNFLAA 663
Cdd:cd05958    79 LDKARITVALCAHALTAS---DDICILA---------------------------FTSGTTGAPKATM---HFHRDPLAS 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  664 mAERFP-----LTAEDRVLATTTVSFDIA-GLELYLPLRSGAGVVLADADTARNpaaLIDLAGRHRVTLAQATPTLWQAL 737
Cdd:cd05958   126 -ADRYAvnvlrLREDDRFVGSPPLAFTFGlGGVLLFPFGVGASGVLLEEATPDL---LLSAIARYKPTVLFTAPTAYRAM 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  738 V--PELSGPALAGIRVLV-GGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSgpTSEDSIRRGSIGVPIDNTQVYVL 813
Cdd:cd05958   202 LahPDAAGPDLSSLRKCVsAGEALPAALHRAWKEAtGIPIIDGIGSTEMFHIFIS--ARPGDARPGATGKPVPGYEAKVV 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  814 DANLHPAPIGVLGELHIAGEglaRGYWNrpglTAEKFLPDPFGppGTRMYrTGDLVRWSAAGDLEYLGRTDHQVKLRGFR 893
Cdd:cd05958   280 DDEGNPVPDGTIGRLAVRGP---TGCRY----LADKRQRTYVQ--GGWNI-TGDTYSRDPDGYFRHQGRSDDMIVSGGYN 349

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  894 IELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPD-----ALRTELSRTLPDYMIPAVIVPVPDFPTTP 968
Cdd:cd05958   350 IAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGpvlarELQDHAKAHIAPYKYPRAIEFVTELPRTA 429


                  ....*....
gi 653678460  969 NGKLDRAAL 977
Cdd:cd05958   430 TGKLQRFAL 438
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 517-977 5.29e-37

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 146.51  E-value: 5.29e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL--SDARpvLVVT 594
Cdd:cd05973     1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLrtSGAR--LVVT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 DAATADRLGpddiTGLVVLeetdtggypateppavpaghsayvIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAED 674
Cdd:cd05973    79 DAANRHKLD----SDPFVM------------------------MFTSGTTGLPKGVPVPLRALAAFGAYLRDAVDLRPED 130

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  675 RVLATTT------VSFDIAGlelylPLRSGAGVVLADAdtARNPAALIDLAGRHRVTLAQATPTLWQAL----VPELSGP 744
Cdd:cd05973   131 SFWNAADpgwaygLYYAITG-----PLALGHPTILLEG--GFSVESTWRVIERLGVTNLAGSPTAYRLLmaagAEVPARP 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  745 ALAGIRVLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIG 823
Cdd:cd05973   204 KGRLRRVSSAGEPLTPEVIRWFDAAlGVPIHDHYGQTELGMVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPG 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  824 VLGELHI--AGEGLA--RGYWNRPGltaekflPDPFGppgtRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEI 899
Cdd:cd05973   284 EPGRLAIdiANSPLMwfRGYQLPDT-------PAIDG----GYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDV 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  900 ETTLINAGPVRRAAAVvreDRPGDLR---LVAYVIPDG-----PVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGK 971
Cdd:cd05973   353 ESALIEHPAVAEAAVI---GVPDPERtevVKAFVVLRGghegtPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGK 429


                  ....*.
gi 653678460  972 LDRAAL 977
Cdd:cd05973   430 IQRFLL 435
starter-C_NRPS cd19533
Starter Condensation domains, found in the first module of nonribosomal peptide synthetases ...
 1083-1499 1.01e-36

Starter Condensation domains, found in the first module of nonribosomal peptide synthetases (NRPSs); Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. While standard C-domains catalyze peptide bond formation between two amino acids, an initial, ('starter') C-domain may instead acylate an amino acid with a fatty acid. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380456 [Multi-domain]  Cd Length: 419  Bit Score: 145.20  E-value: 1.01e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1083 LPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPgPDGMPVQVADPAGGGAA-- 1160
Cdd:cd19533     2 LPLTSAQRGVWFAEQLDPEGSIYNLAEYLEITGPVDLAVLERALRQVIAEAETLRLRFTE-EEGEPYQWIDPYTPVPIrh 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1161 --LTErDAAPGTDLAQLLLAEAALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHR 1238
Cdd:cd19533    81 idLSG-DPDPEGAAQQWMQEDLRKPLPLDNDPLFRHALFTLGDNRHFWYQRVHHIVMDGFSFALFGQRVAEIYTALLKGR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1239 EPElDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVA-TEVAGDLPRPATPGGGGGAVefaLAPRQIQGIRELA 1317
Cdd:cd19533   160 PAP-PAPFGSFLDLVEEEQAYRQSERFERDRAFWTEQFEDLPEpVSLARRAPGRSLAFLRRTAE---LPPELTRTLLEAA 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1318 RRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADAL 1397
Cdd:cd19533   236 EAHGASWPSFFIALVAAYLHRLTGANDVVLGVPVMGRLGAAARQTPGMVANTLPLRLTVDPQQTFAELVAQVSRELRSLL 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1398 AHQELPFEHLVADRG--AGNGPLFRIMYSFSSQEPAGRsAGDVDLEPVPV---PVTtckfDLVLTVVD--GGSTLEGVLE 1470
Cdd:cd19533   316 RHQRYRYEDLRRDLGltGELHPLFGPTVNYMPFDYGLD-FGGVVGLTHNLssgPTN----DLSIFVYDrdDESGLRIDFD 390

                         410       420
                  ....*....|....*....|....*....
gi 653678460 1471 YADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19533   391 ANPALYSGEDLARHQERLLRLLEEAAADP 419
PRK06178 PRK06178
acyl-CoA synthetase; Validated
 487-977 1.10e-36

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 148.27  E-value: 1.10e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  487 PLPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGA 566
Cdd:PRK06178   29 PHGERPLTEYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGA 108

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  567 AYLPIDPDFPVERIAYLLSD--------------------------------------ARPVLVVTDAATADRLGPDDIT 608
Cdd:PRK06178  109 VHVPVSPLFREHELSYELNDagaevllaldqlapvveqvraetslrhvivtsladvlpAEPTLPLPDSLRAPRLAAAGAI 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  609 GLVVLEETDTGGYPAtEPPAVPAghSAYVIYTSGSTGRPKGVVITRAaldNFLAAMAERFPLT---AEDRVLATTTVSFD 685
Cdd:PRK06178  189 DLLPALRACTAPVPL-PPPALDA--LAALNYTGGTTGMPKGCEHTQR---DMVYTAAAAYAVAvvgGEDSVFLSFLPEFW 262

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  686 IAG--LELYLPLRSGAGVVLadadTAR-NPAALIDLAGRHRVTLA-----QATPTLWQALVPELSGPALAGIRVLVGGEA 757
Cdd:PRK06178  263 IAGenFGLLFPLFSGATLVL----LARwDAVAFMAAVERYRVTRTvmlvdNAVELMDHPRFAEYDLSSLRQVRVVSFVKK 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  758 LPAELARRLGDAG----AEVTnmYGPTET----TIwsTSGPTSEDSIRRGS---IGVPIDNTQVYVLDANLH-PAPIGVL 825
Cdd:PRK06178  339 LNPDYRQRWRALTgsvlAEAA--WGMTEThtcdTF--TAGFQDDDFDLLSQpvfVGLPVPGTEFKICDFETGeLLPLGAE 414

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  826 GELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLIN 905
Cdd:PRK06178  415 GEIVVRTPSLLKGYWNKPEATAEALRDG--------WLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQ 486

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  906 AGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVpVPDFPTTPNGKLDRAAL 977
Cdd:PRK06178  487 HPAVLGSAVVGRPDPDKGQVPVAFVQlkPGADLTAAALQAWCRENMAVYKVPEIRI-VDALPMTATGKVRKQDL 559
E_NRPS cd19534
Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 46-346 1.49e-36

Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Epimerization (E) domains of nonribosomal peptide synthetases (NRPS) flip the chirality of the end amino acid of a peptide being manufactured by the NRPS. E-domains are homologous to the Condensation (C) domains. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Specialized tailoring NRPS domains such as E-domains greatly increase the range of possible peptide products created by the NRPS machinery. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the E-domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380457 [Multi-domain]  Cd Length: 428  Bit Score: 145.09  E-value: 1.49e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   46 PVPLAPGQAgiWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGepVQQTAP---AAPV 122
Cdd:cd19534     1 EVPLTPIQR--WFFEQNLAGRHHFNQSVLLRVPQGLDPDALRQALRALVEHHDALRMRFRREDGG--WQQRIRgdvEELF 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  123 ALPVIDVTEADLPQALRTAAEQ---PFDLERGPLLRACL-HRIGAKQHVLLVtVHHIVLDGWSCGLLLADLARAYAGELP 198
Cdd:cd19534    77 RLEVVDLSSLAQAAAIEALAAEaqsSLDLEEGPLLAAALfDGTDGGDRLLLV-IHHLVVDGVSWRILLEDLEAAYEQALA 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  199 AGVAA----PAFRDVAAWQHGRLAAGELDDQLRYWRQRLAGLPdLEIPGDR-QRPADRDwrahSVRREL-PAATEAAVRR 272
Cdd:cd19534   156 GEPIPlpskTSFQTWAELLAEYAQSPALLEELAYWRELPAADY-WGLPKDPeQTYGDAR----TVSFTLdEEETEALLQE 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  273 LAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGvpVAGRGRTDLESV------VGLFAGMVPVRLDLTGRPSFDEVLSRT 346
Cdd:cd19534   231 ANAAYRTEINDLLLAALALAFQDWTGRAPPAIF--LEGHGREEIDPGldlsrtVGWFTSMYPVVLDLEASEDLGDTLKRV 308
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
 503-977 2.43e-36

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 146.11  E-value: 2.43e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  503 RTPGAVAVI--EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERI 580
Cdd:cd05923    13 RAPDACAIAdpARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAEL 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  581 AYLLSDARPVLVVTdaaTADRLGPDDITGLVVLEET---------DTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVV 651
Cdd:cd05923    93 AELIERGEMTAAVI---AVDAQVMDAIFQSGVRVLAlsdlvglgePESAGPLIEDPPREPEQPAFVFYTSGTTGLPKGAV 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITRAALDNFLAAMAERFPLT--AEDRVLATTTVSFDIAGLELYLPLRSGAG-VVLADADTARNPAALIDlagRHRVTLAQ 728
Cdd:cd05923   170 IPQRAAESRVLFMSTQAGLRhgRHNVVLGLMPLYHVIGFFAVLVAALALDGtYVVVEEFDPADALKLIE---QERVTSLF 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  729 ATPTLWQALVP--ELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTiwstsGPTSEDSIRRGSIGVP 804
Cdd:cd05923   247 ATPTHLDALAAaaEFAGLKLSSLRhVTFAGATMPDAVLERVNQHlPGEKVNIYGTTEAM-----NSLYMRDARTGTEMRP 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  805 IDNTQVY---VLDANLHPAPIGVLGELHIAGEGLA--RGYWNRPGLTAEKfLPDpfgppgtRMYRTGDLVRWSAAGDLEY 879
Cdd:cd05923   322 GFFSEVRivrIGGSPDEALANGEEGELIVAAAADAafTGYLNQPEATAKK-LQD-------GWYRTGDVGYVDPSGDVRI 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  880 LGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAYVIPDGPVAPDALRTE-LSRTLPDYMIPAV 957
Cdd:cd05923   394 LGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIgVADERWGQSVTACVVPREGTLSADELDQFcRASELADFKRPRR 473

                         490       500
                  ....*....|....*....|
gi 653678460  958 IVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05923   474 YFFLDELPKNAMNKVLRRQL 493
PRK09088 PRK09088
acyl-CoA synthetase; Validated
 502-977 7.59e-36

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 144.56  E-value: 7.59e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIE--GDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVER 579
Cdd:PRK09088    6 RLQPQRLAAVDlaLGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASE 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  580 IAYLLSDARPVLVVTDAATAD-RLGPDDITGLVVLEETDTggyPATEPPAvPAGHSAYVIYTSGSTGRPKGVVITRAALD 658
Cdd:PRK09088   86 LDALLQDAEPRLLLGDDAVAAgRTDVEDLAAFIASADALE---PADTPSI-PPERVSLILFTSGTSGQPKGVMLSERNLQ 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  659 NFLAAMAERFPLTAEDRVLATTTVsFDIAGL--ELYLPLRSGAGVVLADA-DTARNPAALIDLAgrHRVTLAQATPTLWQ 735
Cdd:PRK09088  162 QTAHNFGVLGRVDAHSSFLCDAPM-FHIIGLitSVRPVLAVGGSILVSNGfEPKRTLGRLGDPA--LGITHYFCVPQMAQ 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  736 AL--VPELSGPALAGIRVLV-GGEALPAELARRLGDAGAEVTNMYGPTET-TIWSTSGPTSEDSIRRGSIGVPIDNTQVY 811
Cdd:PRK09088  239 AFraQPGFDAAALRHLTALFtGGAPHAAEDILGWLDDGIPMVDGFGMSEAgTVFGMSVDCDVIRAKAGAAGIPTPTVQTR 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  812 VLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRG 891
Cdd:PRK09088  319 VVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGW-------FRTGDIARRDADGFFWVVDRKKDMFISGG 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  892 FRIELGEIETTLINAGPVRRAAAV-VREDRPGDL-RLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPN 969
Cdd:PRK09088  392 ENVYPAEIEAVLADHPGIRECAVVgMADAQWGEVgYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTAS 471


                  ....*...
gi 653678460  970 GKLDRAAL 977
Cdd:PRK09088  472 GKLQKARL 479
DCL_NRPS-like cd19536
DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal ...
 1083-1499 9.31e-36

DCL-type Condensation domains of nonribosomal peptide synthetases (NRPSs), such as terminal fungal CT domains and Dual Epimerization/Condensation (E/C) domains; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type [D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L))], which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380459 [Multi-domain]  Cd Length: 419  Bit Score: 142.59  E-value: 9.31e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1083 LPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAgGGAALT 1162
Cdd:cd19536     2 YPLSSLQEGMLFHSLLNPGGSVYLHNYTYTVGRRLNLDLLLEALQVLIDRHDILRTSFIEDGLGQPVQVVHRQ-AQVPVT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1163 ERDAAPGTDLAQL----LLAEAALGFTLATEHPLRAVLWRLDEREHVLL-LTAHHVAVDAWSMDLIQRDLTELYAADTGH 1237
Cdd:cd19536    81 ELDLTPLEEQLDPlrayKEETKIRRFDLGRAPLVRAALVRKDERERFLLvISDHHSILDGWSLYLLVKEILAVYNQLLEY 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1238 REPELdEPALAQADYAVWQRQSAQRgryAAELDFWRTELTGAVATEVAGdlPRPATPGGGGGAVEFALAPRQIQGIRELA 1317
Cdd:cd19536   161 KPLSL-PPAQPYRDFVAHERASIQQ---AASERYWREYLAGATLATLPA--LSEAVGGGPEQDSELLVSVPLPVRSRSLA 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1318 RRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNPRLDNLVGCLVNTVVLRGDLSGApTFHELLRRTHARTAD 1395
Cdd:cd19536   235 KRSGIPLSTLLLAAWALVLSRHSGSDDVVFGTVVHGRseETTGAERLLGLFLNTLPLRVTLSEE-TVEDLLKRAQEQELE 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1396 ALAHQELPFeHLVADRGAGNgPLFRIM-------YSFSSQEPAGRSAGDVDLEPVPVPVTtckFDLVLTVVDGGSTLEGV 1468
Cdd:cd19536   314 SLSHEQVPL-ADIQRCSEGE-PLFDSIvnfrhfdLDFGLPEWGSDEGMRRGLLFSEFKSN---YDVNLSVLPKQDRLELK 388

                         410       420       430
                  ....*....|....*....|....*....|.
gi 653678460 1469 LEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19536   389 LAYNSQVLDEEQAQRLAAYYKSAIAELATAP 419
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
502-977 1.00e-35

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 144.23  E-value: 1.00e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMER-GAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERI 580
Cdd:PRK06839   13 YLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENEL 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  581 AYLLSDA--RPVLV-VTDAATADRLG-------PDDITGLVVLEETDTGGYpatEPPAvpaGHSAYVI-YTSGSTGRPKG 649
Cdd:PRK06839   93 IFQLKDSgtTVLFVeKTFQNMALSMQkvsyvqrVISITSLKEIEDRKIDNF---VEKN---ESASFIIcYTSGTTGKPKG 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  650 VVITRA-----ALDNFLAamaerFPLTAEDRVLATTTVsFDIAGLELY-LP-LRSGAGVVLADAdtaRNPAALIDLAGRH 722
Cdd:PRK06839  167 AVLTQEnmfwnALNNTFA-----IDLTMHDRSIVLLPL-FHIGGIGLFaFPtLFAGGVIIVPRK---FEPTKALSMIEKH 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLWQALV--PELSGPALAGIRVLV-GGEALPAELARRLGDAGAEVTNMYGPTETTiwstsgPT----SEDS 795
Cdd:PRK06839  238 KVTVVMGVPTIHQALIncSKFETTNLQSVRWFYnGGAPCPEELMREFIDRGFLFGQGFGMTETS------PTvfmlSEED 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  796 IRR--GSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSA 873
Cdd:PRK06839  312 ARRkvGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEETIQDGWL--------CTGDLARVDE 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  874 AGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVRED-RPGDLRLVAYVIPDGPVAPDA-LRTELSRTLPD 951
Cdd:PRK06839  384 DGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHvKWGEIPIAFIVKKSSSVLIEKdVIEHCRLFLAK 463

                         490       500
                  ....*....|....*....|....*.
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06839  464 YKIPKEIVFLKELPKNATGKIQKAQL 489
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
 1552-2033 1.45e-35

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 143.61  E-value: 1.45e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1552 TGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADT 1631
Cdd:cd05926    10 GSTPALTYADLAELVDDLARQLA--ALG---IKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLAD 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1632 VTPALVLTRTGQGG-CLPGA--------EVFGDVPVVALDRVADRLTAMPD----QRPEVTVDPRGLAYSIFTSGSTGQP 1698
Cdd:cd05926    85 LGSKLVLTPKGELGpASRAAsklglailELALDVGVLIRAPSAESLSNLLAdkknAKSEGVPLPDDLALILHTSGTTGRP 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1699 KGVAVEHIGIVRYLSWAAASYPTAGRRGTLA-------HSSVGfdltmsALFEPLVSGRGVTLMPA-DATL--ADLAEE- 1767
Cdd:cd05926   165 KGVPLTHRNLAASATNITNTYKLTPDDRTLVvmplfhvHGLVA------SLLSTLAAGGSVVLPPRfSASTfwPDVRDYn 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1768 --------------LSGPLAYDYIRltPSHLRHlvghwtgqelppaARGwvvGGETLDPALVKQLlELRPDAEVINHYGP 1833
Cdd:cd05926   239 atwytavptihqilLNRPEPNPESP--PPKLRF-------------IRS---CSASLPPAVLEAL-EATFGAPVLEAYGM 299

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1834 TETVIGRVVHPVreagELAVDSPLPLGRPLGeTRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPa 1913
Cdd:cd05926   300 TEAAHQMTSNPL----PPGPRKPGSVGKPVG-VEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDG- 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1914 gepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQ---QLVGWFIPAEDH 1989
Cdd:cd05926   374 ------WFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFgVPDEkygEEVAAAVVLREG 447

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 653678460 1990 PPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05926   448 ASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKV 491
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
 1532-2033 2.88e-35

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 143.40  E-value: 2.88e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGG 1611
Cdd:PRK06187    7 TIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALR--ALG---VKKGDRVAVFDWNSHEYLEAYFAVPKIGA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1612 YFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAE----------VFGDVPVVALDRVADRLTAM----PDQRPE 1677
Cdd:PRK06187   82 VLHPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILpqlptvrtviVEGDGPAAPLAPEVGEYEELlaaaSDTFDF 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1678 VTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRyLSWAAASYPTAGRRGT------LAHS---SVGFDLTMSAL------ 1742
Cdd:PRK06187  162 PDIDENDAAAMLYTSGTTGHPKGVVLSHRNLFL-HSLAVCAWLKLSRDDVylvivpMFHVhawGLPYLALMAGAkqvipr 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1743 -FEP-----LVSGRGVTLMPADAT-LADLaeeLSGPLAY--DYirltpSHLRHLVghwtgqelppaargwvVGGETLDPA 1813
Cdd:PRK06187  241 rFDPenlldLIETERVTFFFAVPTiWQML---LKAPRAYfvDF-----SSLRLVI----------------YGGAALPPA 296

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1814 LVKQLLElRPDAEVINHYGPTETV-IGRVVHPvrEAGELAvDSPLPL--GRPLGETRLQVLDAWLEAVPV--GAVGELFI 1888
Cdd:PRK06187  297 LLREFKE-KFGIDLVQGYGMTETSpVVSVLPP--EDQLPG-QWTKRRsaGRPLPGVEARIVDDDGDELPPdgGEVGEIIV 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1889 GGDGIARGYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV- 1967
Cdd:PRK06187  373 RGPWLMQGYWNRPEATAETIDGG--------WLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVa 444

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1968 EQAVVLVRDQQlvgW------FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK06187  445 EVAVIGVPDEK---WgerpvaVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVL 513
PRK06145 PRK06145
acyl-CoA synthetase; Validated
 500-972 1.06e-34

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 141.18  E-value: 1.06e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  500 WCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVER 579
Cdd:PRK06145   11 HARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADE 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  580 IAYLLSDARPVLVVTD---AATADRLGPDDITGLVVLEETD---TGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVIT 653
Cdd:PRK06145   91 VAYILGDAGAKLLLVDeefDAIVALETPKIVIDAAAQADSRrlaQGGLEIPPQAAVAPTDLVRLMYTSGTTDRPKGVMHS 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  654 raaLDNFLAAMAER---FPLTAEDRVLATTTV----SFDIAGLelylplrsgagVVLADADTAR-----NPAALIDLAGR 721
Cdd:PRK06145  171 ---YGNLHWKSIDHviaLGLTASERLLVVGPLyhvgAFDLPGI-----------AVLWVGGTLRihrefDPEAVLAAIER 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  722 HRVTLAQATPTLWQAL--VPELSGPALAGIR-VLVGGEALPAELARRLGD--AGAEVTNMYGPTETTIWSTSGPTSEDSI 796
Cdd:PRK06145  237 HRLTCAWMAPVMLSRVltVPDRDRFDLDSLAwCIGGGEKTPESRIRDFTRvfTRARYIDAYGLTETCSGDTLMEAGREIE 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  797 RRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGD 876
Cdd:PRK06145  317 KIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGDWF--------RSGDVGYLDEEGF 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  877 LEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVI--PDGPVAPDALRTELSRTLPDYM 953
Cdd:PRK06145  389 LYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIgVHDDRWGE-RITAVVVlnPGATLTLEALDRHCRQRLASFK 467

                         490
                  ....*....|....*....
gi 653678460  954 IPAVIVPVPDFPTTPNGKL 972
Cdd:PRK06145  468 VPRQLKVRDELPRNPSGKV 486
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
 1548-2033 1.40e-34

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 139.73  E-value: 1.40e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGDRSLTFAGLDAQANRLAHALHtgalGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTR 1627
Cdd:cd05941     3 IAIVDDGDSITYADLVARAARLANRLL----ALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEY 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1628 IADTVTPALVLTRtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvdprglAYSIFTSGSTGQPKGVAVEHIG 1707
Cdd:cd05941    79 VITDSEPSLVLDP---------------------------------------------ALILYTSGTTGRPKGVVLTHAN 113

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1708 I---VRYLS--WAAASyptagrRGTLAHssvGFDL-----TMSALFEPLVSGRGVTLMPA-DATLADLAEELSGPLAYD- 1775
Cdd:cd05941   114 LaanVRALVdaWRWTE------DDVLLH---VLPLhhvhgLVNALLCPLFAGASVEFLPKfDPKEVAISRLMPSITVFMg 184

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1776 ----YIRLTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVV--HPVREAG 1849
Cdd:cd05941   185 vptiYTRLLQYYEAHFTDPQFARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALSNplDGERRPG 264

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 elAVDSPLPlgrplG-ETRLqVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepgaRMYRTGDLVR 1928
Cdd:cd05941   265 --TVGMPLP-----GvQARI-VDEETGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDD-------GWFKTGDLGV 329

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1929 WRGDGLLDFVGRV-DDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD----QQLVGWFIPAEDHPPVTVSALRRFCA 2002
Cdd:cd05941   330 VDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVsECAVIGVPDpdwgERVVAVVVLRAGAAALSLEELKEWAK 409

                         490       500       510
                  ....*....|....*....|....*....|.
gi 653678460 2003 EQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05941   410 QRLAPYKRPRRLILVDELPRNAMGKVNKKEL 440
PRK07788 PRK07788
acyl-CoA synthetase; Validated
 480-981 1.48e-34

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 141.60  E-value: 1.48e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  480 RWNDTARPLPAASlrerfqqwcRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLL 559
Cdd:PRK07788   47 RYGPFAGLVAHAA---------RRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALY 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  560 AVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRLG--PDDITGLVVL-EETDTGGYPA------------- 623
Cdd:PRK07788  118 AAGKVGARIILLNTGFSGPQLAEVAAREGVKALVYDDEFTDLLSalPPDLGRLRAWgGNPDDDEPSGstdetlddliags 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  624 -TEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTV--SFDIAGLELYLPLRSgaG 700
Cdd:PRK07788  198 sTAPLPKPPKPGGIVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMfhATGWAHLTLAMALGS--T 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  701 VVLadadtAR--NPAALIDLAGRHRVTLAQATPTLWQ---ALVPELSG-PALAGIR-VLVGGEALPAELARRLGDA-GAE 772
Cdd:PRK07788  276 VVL-----RRrfDPEATLEDIAKHKATALVVVPVMLSrilDLGPEVLAkYDTSSLKiIFVSGSALSPELATRALEAfGPV 350

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  773 VTNMYGPTETTIWSTSGPtSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYwnrpgltaekflP 852
Cdd:PRK07788  351 LYNLYGSTEVAFATIATP-EDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGY------------T 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  853 DPFGPPGTR-MYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI 931
Cdd:PRK07788  418 DGRDKQIIDgLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVV 497

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460  932 --PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPD 981
Cdd:PRK07788  498 kaPGAALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1566-2033 2.18e-34

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 139.50  E-value: 2.18e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1566 ANRLAHALHTGALgappVGPETPVLLLLDRSpELVVAMLAVLKAGGY----FIPVDPGYPMARLTRIADTVTPALVLTRT 1641
Cdd:cd05922     3 VSAAASALLEAGG----VRGERVVLILPNRF-TYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1642 GQGGCL-PGAEVFGDVPVVALdrvADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIvryLSWAAASYP 1720
Cdd:cd05922    78 GAADRLrDALPASPDPGTVLD---ADGIRAARASAPAHEVSHEDLALLLYTSGSTGSPKLVRLSHQNL---LANARSIAE 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1721 TAGRRGT---LAHSSVGFDLTMSALFEPLVSGRGVTL----MPADATLADLAEELSGPLAydyirLTPSHLRHLvghwTG 1793
Cdd:cd05922   152 YLGITADdraLTVLPLSYDYGLSVLNTHLLRGATLVLtndgVLDDAFWEDLREHGATGLA-----GVPSTYAML----TR 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1794 QELPPAA----RGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPvreaGELAVDSPLPLGRPLGETRLQ 1869
Cdd:cd05922   223 LGFDPAKlpslRYLTQAGGRLPQETIARLRELLPGAQVYVMYGQTEATRRMTYLP----PERILEKPGSIGLAIPGGEFE 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1870 VL--DAWLEavPVGAVGELFIGGDGIARGYLGRPALTAEKflpdpaGEPGARMYrTGDLVRWRGDGLLDFVGRVDDQVKL 1947
Cdd:cd05922   299 ILddDGTPT--PPGEPGEIVHRGPNVMKGYWNDPPYRRKE------GRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKL 369

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1948 RGYRIELGEIEARMAEHPGVEQAVVLVRDQQLV-GWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHG 2026
Cdd:cd05922   370 FGNRISPTEIEAAARSIGLIIEAAAVGLPDPLGeKLALFVTAPDKIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASG 449


                  ....*..
gi 653678460 2027 KVNHRAL 2033
Cdd:cd05922   450 KVDYAAL 456
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
 517-981 5.71e-34

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 137.63  E-value: 5.71e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:cd05969     1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  597 ATADRLGPDDitglvvleetdtggypateppavpaghSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRV 676
Cdd:cd05969    81 ELYERTDPED---------------------------PTLLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDLHPDDIY 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  677 LATTT---VSFDIAGLelYLPLRSGAGVVLADADTarNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPA----LAGI 749
Cdd:cd05969   134 WCTADpgwVTGTVYGI--WAPWLNGVTNVVYEGRF--DAESWYGIIERVKVTVWYTAPTAIRMLMKEGDELArkydLSSL 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 R-VLVGGEALPAELARrlgdAGAEVTNM-----YGPTETTIWSTSGPTSEDsIRRGSIGVPIDNTQVYVLDANLHPAPIG 823
Cdd:cd05969   210 RfIHSVGEPLNPEAIR----WGMEVFGVpihdtWWQTETGSIMIANYPCMP-IKPGSMGKPLPGVKAAVVDENGNELPPG 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  824 VLGELHIAGE--GLARGYWNRPGLTAEKFLpdpfgppgTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIET 901
Cdd:cd05969   285 TKGILALKPGwpSMFRGIWNDEERYKNSFI--------DGWYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVES 356

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  902 TLINAGPVRRAAAVVREDRPGDLRLVAYV-IPDGPVAPDALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:cd05969   357 ALMEHPAVAEAGVIGKPDPLRGEIIKAFIsLKEGFEPSDELKEEIinfvRQKLGAHVAPREIEFVDNLPKTRSGKIMRRV 436


                  ....*
gi 653678460  977 LPAPD 981
Cdd:cd05969   437 LKAKE 441
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
 490-977 1.22e-33

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 138.57  E-value: 1.22e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  490 AASLRERFQQWCRRTPGA----VAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAG 565
Cdd:cd05906     9 PRTLLELLLRAAERGPTKgityIDADGSEEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAG 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  566 --AAYLPIDPDFP-----VERIAYLLSDARPVLVVTDAATADRLGP-------DDITGLVVLEETDTGGypatEPPAVPA 631
Cdd:cd05906    89 fvPAPLTVPPTYDepnarLRKLRHIWQLLGSPVVLTDAELVAEFAGletlsglPGIRVLSIEELLDTAA----DHDLPQS 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  632 GHS--AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLatTTVSFD-IAGL-ELYL-PLRSGAGVVLADA 706
Cdd:cd05906   165 RPDdlALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFL--NWVPLDhVGGLvELHLrAVYLGCQQVHVPT 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  707 DTA-RNPAALIDLAGRHRVTLAQAtP----TLWQALVPELSGPA--LAGIRVLV-GGEALPAE----LARRLGDAGAEVT 774
Cdd:cd05906   243 EEIlADPLRWLDLIDRYRVTITWA-PnfafALLNDLLEEIEDGTwdLSSLRYLVnAGEAVVAKtirrLLRLLEPYGLPPD 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  775 NM---YGPTET---TIWSTSGPT--SEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLT 846
Cdd:cd05906   322 AIrpaFGMTETcsgVIYSRSFPTydHSQALEFVSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEAN 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  847 AEKFLPDPFgppgtrmYRTGDLVrWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR---AAAVVREDRPGD 923
Cdd:cd05906   402 AEAFTEDGW-------FRTGDLG-FLDNGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPsftAAFAVRDPGAET 473

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 653678460  924 LRLV-----AYVIPDGPVAP-DALRTELSRTL---PDYMIPaviVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05906   474 EELAiffvpEYDLQDALSETlRAIRSVVSREVgvsPAYLIP---LPKEEIPKTSLGKIQRSKL 533
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
517-990 1.24e-33

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 140.94  E-value: 1.24e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDA 596
Cdd:PRK06060   31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEPALVVTSD 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  597 ATADRLGPDDITGLVVLEETDTGGYPATEPPaVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAER-FPLTAEDR 675
Cdd:PRK06060  111 ALRDRFQPSRVAEAAELMSEAARVAPGGYEP-MGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKaLRLTPEDT 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATTTVSFDIA-GLELYLPLRSGAGVVLADADTARNPAALidLAGRHRVTLAQATPTLWQALVPELSGPALAGIR-VLV 753
Cdd:PRK06060  190 GLCSARMYFAYGlGNSVWFPLATGGSAVINSAPVTPEAAAI--LSARFGPSVLYGVPNFFARVIDSCSPDSFRSLRcVVS 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  754 GGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE 833
Cdd:PRK06060  268 AGEALELGLAERLMEFFGGIPILDGIGSTEVGQTFVSNRVDEWRLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGP 347

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  834 GLARGYWNRPG--LTAEKFLpdpfgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR 911
Cdd:PRK06060  348 AIAKGYWNRPDspVANEGWL------------DTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAE 415

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  912 AAAVVREDRPGDLRLVAYVIP------DGPVAPDALRTELSRtLPDYMIPAVIVPVPDFPTTPNGKLDRAALPApdygtR 985
Cdd:PRK06060  416 AAVVAVRESTGASTLQAFLVAtsgatiDGSVMRDLHRGLLNR-LSAFKVPHRFAVVDRLPRTPNGKLVRGALRK-----Q 489


                  ....*
gi 653678460  986 STARP 990
Cdd:PRK06060  490 SPTKP 494
PRK07798 PRK07798
acyl-CoA synthetase; Validated
 505-973 1.41e-33

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 138.48  E-value: 1.41e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PRK07798   17 PDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELRYLL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLGP--DDITGLVVLEETDTGGYPATEPPAVP------AGHSA-----------YVIYTSGSTG 645
Cdd:PRK07798   97 DDSDAVALVYEREFAPRVAEvlPRLPKLRTLVVVEDGSGNDLLPGAVDyedalaAGSPErdfgerspddlYLLYTGGTTG 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  646 RPKGVVITRAalDNFLAAMAERFPLTAE---------DRVLATT-TVSFDIAglelylPLRSGAG-------------VV 702
Cdd:PRK07798  177 MPKGVMWRQE--DIFRVLLGGRDFATGEpiedeeelaKRAAAGPgMRRFPAP------PLMHGAGqwaafaalfsgqtVV 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  703 LADADTArNPAALIDLAGRHRVTL------AQATPtLWQALVPElSGPALAGIRVLV-GGEALPAELARRLGDA--GAEV 773
Cdd:PRK07798  249 LLPDVRF-DADEVWRTIEREKVNVitivgdAMARP-LLDALEAR-GPYDLSSLFAIAsGGALFSPSVKEALLELlpNVVL 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  774 TNMYGPTET----TIWSTSGPTSEDSIRrgsigVPIDNTQVyVLDANLHPAPIGVLGELHIAGEG-LARGYWNRPGLTAE 848
Cdd:PRK07798  326 TDSIGSSETgfggSGTVAKGAVHTGGPR-----FTIGPRTV-VLDEDGNPVEPGSGEIGWIARRGhIPLGYYKDPEKTAE 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  849 KFlpdpFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVRED-RPGDlRLV 927
Cdd:PRK07798  400 TF----PTIDGVRYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDeRWGQ-EVV 474

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460  928 AYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:PRK07798  475 AVVQLREGARPDlaELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKAD 522
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
491-977 2.60e-33

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 137.96  E-value: 2.60e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  491 ASLRERFQQWCRRTPGAVAVI-EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYL 569
Cdd:PRK06087   23 ASLADYWQQTARAMPDKIAVVdNHGASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSV 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  570 PIDPDFPVERIAYLLS--------------DARPVLVVTD----------AATADRLGPDdiTGLVVLEETDTGGYPATE 625
Cdd:PRK06087  103 PLLPSWREAELVWVLNkcqakmffaptlfkQTRPVDLILPlqnqlpqlqqIVGVDKLAPA--TSSLSLSQIIADYEPLTT 180

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  626 PPAVPAGHSAYVIYTSGSTGRPKGVVITRaalDNFLA---AMAERFPLTAEDRVLATTTVSFDIAGLE-LYLPLRSGAGV 701
Cdd:PRK06087  181 AITTHGDELAAVLFTSGTEGLPKGVMLTH---NNILAserAYCARLNLTWQDVFMMPAPLGHATGFLHgVTAPFLIGARS 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  702 VLADadtARNPAALIDLAGRHRVTLAQ-ATPTLWQALVP-ELSGPALAGIRV-LVGGEALPAELARRLGDAGAEVTNMYG 778
Cdd:PRK06087  258 VLLD---IFTPDACLALLEQQRCTCMLgATPFIYDLLNLlEKQPADLSALRFfLCGGTTIPKKVARECQQRGIKLLSVYG 334

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  779 PTETTIWSTSGPtsEDSIRR--GSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFg 856
Cdd:PRK06087  335 STESSPHAVVNL--DDPLSRfmHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELTARALDEEGW- 411

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  857 ppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVRED-RPGDlRLVAYVIPDGP 935
Cdd:PRK06087  412 ------YYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDeRLGE-RSCAYVVLKAP 484

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 653678460  936 VAPDALRTELS----RTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06087  485 HHSLTLEEVVAffsrKRVAKYKYPEHIVVIDKLPRTASGKIQKFLL 530
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
 502-977 4.33e-33

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 137.11  E-value: 4.33e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIE------GDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:PRK13295   35 ASCPDKTAVTAvrlgtgAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIF 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  576 PVERIAYLLSDARP-VLVVTD-------AATADRLGPD--DITGLVVLEETDTGGYPA--TEP--------PAVPAGHS- 634
Cdd:PRK13295  115 RERELSFMLKHAESkVLVVPKtfrgfdhAAMARRLRPElpALRHVVVVGGDGADSFEAllITPaweqepdaPAILARLRp 194

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 -----AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFD---IAGLElyLPLRSGAGVVLADa 706
Cdd:PRK13295  195 gpddvTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQtgfMYGLM--MPVMLGATAVLQD- 271

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  707 dtARNPAALIDLAGRHRVTLAQA-TPTLWQ-ALVPELSGPALAGIRV-LVGGEALPAELARRLGDA-GAEVTNMYGPTET 782
Cdd:PRK13295  272 --IWDPARAAELIRTEGVTFTMAsTPFLTDlTRAVKESGRPVSSLRTfLCAGAPIPGALVERARAAlGAKIVSAWGMTEN 349

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  783 TIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEkflpDPFGppgtrM 862
Cdd:PRK13295  350 GAVTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQVRGCSNFGGYLKRPQLNGT----DADG-----W 420

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  863 YRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP------DGPV 936
Cdd:PRK13295  421 FDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPrpgqslDFEE 500

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 653678460  937 APDALRtelSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK13295  501 MVEFLK---AQKVAKQYIPERLVVRDALPRTPSGKIQKFRL 538
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
498-974 5.40e-33

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 135.86  E-value: 5.40e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  498 QQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPV 577
Cdd:PRK03640    9 KQRAFLTPDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSR 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  578 ERIAYLLSDARPVLVVTDAATADRLGPDditGLVVLEETDTGGYPATEP-PAVPAGHSAYVIYTSGSTGRPKGVVITRAa 656
Cdd:PRK03640   89 EELLWQLDDAEVKCLITDDDFEAKLIPG---ISVKFAELMNGPKEEAEIqEEFDLDEVATIMYTSGTTGKPKGVIQTYG- 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  657 lDNFLAAM--AERFPLTAEDRVLATTTVsFDIAGLELYlpLRS---GAGVVLADadtARNPAALIDLAGRHRVTLAQATP 731
Cdd:PRK03640  165 -NHWWSAVgsALNLGLTEDDCWLAAVPI-FHISGLSIL--MRSviyGMRVVLVE---KFDAEKINKLLQTGGVTIISVVS 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQALVPEL-SGPALAGIR-VLVGGEALPAELARRLGDAGAEVTNMYGPTET-----TIwstsgpTSEDSIRR-GSIGV 803
Cdd:PRK03640  238 TMLQRLLERLgEGTYPSSFRcMLLGGGPAPKPLLEQCKEKGIPVYQSYGMTETasqivTL------SPEDALTKlGSAGK 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  804 PIDNTQVYVLDaNLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRT 883
Cdd:PRK03640  312 PLFPCELKIEK-DGVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQDGWF--------KTGDIGYLDEEGFLYVLDRR 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  884 DHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDR-----PgdlrlVAYVIPDGPVAPDALRTELSRTLPDYMIPAVI 958
Cdd:PRK03640  383 SDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDkwgqvP-----VAFVVKSGEVTEEELRHFCEEKLAKYKVPKRF 457

                         490
                  ....*....|....*.
gi 653678460  959 VPVPDFPTTPNGKLDR 974
Cdd:PRK03640  458 YFVEELPRNASGKLLR 473
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
 488-972 6.33e-33

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 136.63  E-value: 6.33e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  488 LPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLM-ERGAGAETFVAVLLPRSADLLVTLLAVIKAGA 566
Cdd:PRK08314    7 LPETSLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYYAILRANA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  567 AYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRL----GPDDITGLVVLEETDT----GGYP-----ATEPPAVPAGH 633
Cdd:PRK08314   87 VVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVapavGNLRLRHVIVAQYSDYlpaePEIAvpawlRAEPPLQALAP 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  634 SAYVI-------------------------YTSGSTGRPKGVVIT-RAALDNFLAAmAERFPLTAEDRVLATTTVsFDIA 687
Cdd:PRK08314  167 GGVVAwkealaaglappphtagpddlavlpYTSGTTGVPKGCMHThRTVMANAVGS-VLWSNSTPESVVLAVLPL-FHVT 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  688 GLE--LYLPLRSGAGVVLA---DADTARnpaaliDLAGRHRVTLAQATPTLwqaLVPELSGPALAG-----IRVLVGG-E 756
Cdd:PRK08314  245 GMVhsMNAPIYAGATVVLMprwDREAAA------RLIERYRVTHWTNIPTM---VVDFLASPGLAErdlssLRYIGGGgA 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  757 ALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTseDSIRRGSIGVPIDNTQVYVLD-ANLHPAPIGVLGELHIAGEG 834
Cdd:PRK08314  316 AMPEAVAERLKELtGLDYVEGYGLTETMAQTHSNPP--DRPKLQCLGIPTFGVDARVIDpETLEELPPGEVGEIVVHGPQ 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  835 LARGYWNRPGLTAEKFLP-DpfgppGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAA 913
Cdd:PRK08314  394 VFKGYWNRPEATAEAFIEiD-----GKRFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEAC 468

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  914 AV-VREDRPGDlRLVAYVIPD----GPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK08314  469 VIaTPDPRRGE-TVKAVVVLRpearGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
 507-972 1.13e-32

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 135.03  E-value: 1.13e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  507 AVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSD 586
Cdd:PRK08276    2 AVIMAPSGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDD 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  587 ARPVLVVTDAATAD------RLGPDDITGLVVLEETDTG---------GYPATEPPAVPAGhsAYVIYTSGSTGRPKGVV 651
Cdd:PRK08276   82 SGAKVLIVSAALADtaaelaAELPAGVPLLLVVAGPVPGfrsyeealaAQPDTPIADETAG--ADMLYSSGTTGRPKGIK 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ITR------AALDNFLAAMAERFPLTAEDRVLATTTvsfdiagleLY--LPLRSGAG-------VVLADADTARNPAALI 716
Cdd:PRK08276  160 RPLpgldpdEAPGMMLALLGFGMYGGPDSVYLSPAP---------LYhtAPLRFGMSalalggtVVVMEKFDAEEALALI 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  717 DlagRHRVTLAQATPTLWQ---ALVPEL-SGPALAGIRVLVGGEA-LPAELARRLGD-AGAEVTNMYGPTE----TTIws 786
Cdd:PRK08276  231 E---RYRVTHSQLVPTMFVrmlKLPEEVrARYDVSSLRVAIHAAApCPVEVKRAMIDwWGPIIHEYYASSEgggvTVI-- 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  787 tsgpTSEDSIRR-GSIGVPIDnTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrmyrt 865
Cdd:PRK08276  306 ----TSEDWLAHpGSVGKAVL-GEVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARNPH------------ 368

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  866 gdlvRWSAAGDLEYL---------GRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVIP-DG 934
Cdd:PRK08276  369 ----GWVTVGDVGYLdedgylyltDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFgVPDEEMGE-RVKAVVQPaDG 443

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 653678460  935 PVAPDALRTELSRTLPD----YMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK08276  444 ADAGDALAAELIAWLRGrlahYKCPRSIDFEDELPRTPTGKL 485
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
 1552-2029 4.42e-32

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 133.11  E-value: 4.42e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1552 TGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADT 1631
Cdd:cd05911     6 DTGKELTYAQLRTLSRRLAAGLR--KLG---LKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1632 VTPALVLT--------RTGQGGCLPGAEVF-------GDVPVVALDRVADrLTAMPDQRPEVTVDPRGLAYSIFTSGSTG 1696
Cdd:cd05911    81 SKPKVIFTdpdglekvKEAAKELGPKDKIIvlddkpdGVLSIEDLLSPTL-GEEDEDLPPPLKDGKDDTAAILYSSGTTG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1697 QPKGVAVEHIGIVRYLSWAAASYPTAGRRG-------TLAHSSvGFDLTMSALFeplvsgRGVTL--MPA--DATLADLA 1765
Cdd:cd05911   160 LPKGVCLSHRNLIANLSQVQTFLYGNDGSNdvilgflPLYHIY-GLFTTLASLL------NGATViiMPKfdSELFLDLI 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1766 EELSGPLAYdyirLTPSHLRHLVGHwtgqELPPAA-----RGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGR 1840
Cdd:cd05911   233 EKYKITFLY----LVPPIAAALAKS----PLLDKYdlsslRVILSGGAPLSKELQELLAKRFPNATIKQGYGMTETGGIL 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1841 VVHPVREAGELAVDSPLPLgrplgeTRLQVLDaWL--EAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepga 1918
Cdd:cd05911   305 TVNPDGDDKPGSVGRLLPN------VEAKIVD-DDgkDSLGPNEPGEICVRGPQVMKGYYNNPEATKETFDED------- 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1919 RMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVV----LVRDQQLVGWFIPAEDHPPVTV 1994
Cdd:cd05911   371 GWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVigipDEVSGELPRAYVVRKPGEKLTE 450

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 653678460 1995 SALRRFCAEQLPEFmvpNQW----VALDAFPLTPHGKVN 2029
Cdd:cd05911   451 KEVKDYVAKKVASY---KQLrggvVFVDEIPKSASGKIL 486
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
503-977 6.17e-32

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 133.58  E-value: 6.17e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  503 RTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAylPIDPDFPVERIAy 582
Cdd:PRK10946   35 AASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSE- 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  583 LLSDAR---PVLVVTDAATA--------DRLGPDDITGLVVLEETDTGGY--------PATEPPA--VPAGHSAYVIYTS 641
Cdd:PRK10946  112 LNAYASqiePALLIADRQHAlfsdddflNTLVAEHSSLRVVLLLNDDGEHslddainhPAEDFTAtpSPADEVAFFQLSG 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  642 GSTGRPKgvVITRAALDNFLA--AMAERFPLTAEDRVLATTTVSFDiaglelyLPLRS-GA-GVVLADADT--ARNPAAL 715
Cdd:PRK10946  192 GSTGTPK--LIPRTHNDYYYSvrRSVEICGFTPQTRYLCALPAAHN-------YPMSSpGAlGVFLAGGTVvlAPDPSAT 262

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  716 I--DLAGRHRVTLAQATP---TLW-QALVPELSGPALAGIRVL-VGGEALPAELARRLGDA-GAEVTNMYGPTETTIWST 787
Cdd:PRK10946  263 LcfPLIEKHQVNVTALVPpavSLWlQAIAEGGSRAQLASLKLLqVGGARLSETLARRIPAElGCQLQQVFGMAEGLVNYT 342

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  788 SGPTSEDSIrRGSIGVPI-DNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTG 866
Cdd:PRK10946  343 RLDDSDERI-FTTQGRPMsPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGF-------YCSG 414

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  867 DLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTEL- 945
Cdd:PRK10946  415 DLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEPLKAVQLRRFLr 494

                         490       500       510
                  ....*....|....*....|....*....|..
gi 653678460  946 SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK10946  495 EQGIAEFKLPDRVECVDSLPLTAVGKVDKKQL 526
PRK07514 PRK07514
malonyl-CoA synthase; Validated
 491-977 6.94e-31

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 129.61  E-value: 6.94e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  491 ASLRERFQqwcrrTPGAVAVIEGD-TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYL 569
Cdd:PRK07514    7 DALRAAFA-----DRDAPFIETPDgLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  570 PIDPDFPVERIAYLLSDARPVLVVTDAATADRLGP--------------DDITGlvVLEETDTGGYPATEPPAVPAGHSA 635
Cdd:PRK07514   82 PLNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKiaaaagaphvetldADGTG--SLLEAAAAAPDDFETVPRGADDLA 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 YVIYTSGSTGRPKGVVITRaalDNFLA---AMAERFPLTAEDRVLATTTVsFDIAGL--ELYLPLRSGAGVV-LADADta 709
Cdd:PRK07514  160 AILYTSGTTGRSKGAMLSH---GNLLSnalTLVDYWRFTPDDVLIHALPI-FHTHGLfvATNVALLAGASMIfLPKFD-- 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  710 rnPAALIDLagrhrvtLAQAT-----PTLWQALV--PELSGPALAGIRVLVGGEA-LPAELAR----RLGDAGAEvtnMY 777
Cdd:PRK07514  234 --PDAVLAL-------MPRATvmmgvPTFYTRLLqePRLTREAAAHMRLFISGSApLLAETHRefqeRTGHAILE---RY 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  778 GPTETTIwSTSGPTseDSIRR-GSIGVPIDNTQVYVLD-ANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPF 855
Cdd:PRK07514  302 GMTETNM-NTSNPY--DGERRaGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGF 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  856 gppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIEtTLINAGPVRRAAAVVREDRPgDL--RLVAYVI-- 931
Cdd:PRK07514  379 -------FITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVE-GEIDELPGVVESAVIGVPHP-DFgeGVTAVVVpk 449

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 653678460  932 PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07514  450 PGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLL 495
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
 1684-2033 9.25e-31

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 125.52  E-value: 9.25e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1684 GLAYSIFTSGSTGQPKGVAvehigivryLSWAAAsypTAGRRGTlaHSSVGFDLTMSALFE-PL--VSGRGVTL------ 1754
Cdd:cd17630     1 RLATVILTSGSTGTPKAVV---------HTAANL---LASAAGL--HSRLGFGGGDSWLLSlPLyhVGGLAILVrsllag 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 -MPADATLADLAEELSGPLAYDYIRLTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELRpdAEVINHYGP 1833
Cdd:cd17630    67 aELVLLERNQALAEDLAPPGVTHVSLVPTQLQRLLDSGQGPAALKSLRAVLLGGAPIPPELLERAADRG--IPLYTTYGM 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1834 TETVIGRVVHPVREAGELAVdsplplGRPLGETRLQVLDAwleavpvgavGELFIGGDGIARGYLGRPaltaekfLPDPA 1913
Cdd:cd17630   145 TETASQVATKRPDGFGRGGV------GVLLPGRELRIVED----------GEIWVGGASLAMGYLRGQ-------LVPEF 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1914 GEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRD----QQLVGWFipaED 1988
Cdd:cd17630   202 NEDG--WFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVgVPDeelgQRPVAVI---VG 276

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*
gi 653678460 1989 HPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd17630   277 RGPADPAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRAL 321
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
 1556-2028 2.02e-30

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 127.11  E-value: 2.02e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1556 SLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPA 1635
Cdd:cd05903     1 RLTYSELDTRADRLAAGL--AALG---VGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAK 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1636 LVLTrtgqggclpgAEVFGdvpvvaldrvADRLTAMPDQrpevtvdprgLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWA 1715
Cdd:cd05903    76 VFVV----------PERFR----------QFDPAAMPDA----------VALLLFTSGTTGEPKGVMHSHNTLSASIRQY 125

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1716 AASYPTAGRRGTLAHSSVG-FDLTMSALFEPLVSGRGVTLMP---ADATLADLAEE------LSGPLAYDYIRL---TPS 1782
Cdd:cd05903   126 AERLGLGPGDVFLVASPMAhQTGFVYGFTLPLLLGAPVVLQDiwdPDKALALMREHgvtfmmGATPFLTDLLNAveeAGE 205

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1783 HLRHLvghwtgqelppaaRGWVVGGETLDPALVKQLLELRpDAEVINHYGPTETV-IGRVVHPVREAGELAVDsplplGR 1861
Cdd:cd05903   206 PLSRL-------------RTFVCGGATVPRSLARRAAELL-GAKVCSAYGSTECPgAVTSITPAPEDRRLYTD-----GR 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1862 PLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdPAGepgarMYRTGDLVRWRGDGLLDFVGRV 1941
Cdd:cd05903   267 PLPGVEIKVVDDTGATLAPGVEGELLSRGPSVFLGYLDRPDLTADAA---PEG-----WFRTGDLARLDEDGYLRITGRS 338

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1942 DDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQL---VGWFIPAEDHPPVTVSALRRFC-AEQLPEFMVPNQWVA 2016
Cdd:cd05903   339 KDIIIRGGENIPVLEVEDLLLGHPGViEAAVVALPDERLgerACAVVVTKSGALLTFDELVAYLdRQGVAKQYWPERLVH 418

                         490
                  ....*....|..
gi 653678460 2017 LDAFPLTPHGKV 2028
Cdd:cd05903   419 VDDLPRTPSGKV 430
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
 1530-2033 6.32e-30

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 127.18  E-value: 6.32e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKA 1609
Cdd:COG1021    24 GETLGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLL--ALG---LRPGDRVVVQLPNVAEFVIVFFALFRA 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1610 GgyFIPVD--PGYPMARLTRIADTVTPALVLTRTGQGG------------CLPGAE---VFGDV-PVVALDRvadrLTAM 1671
Cdd:COG1021    99 G--AIPVFalPAHRRAEISHFAEQSEAVAYIIPDRHRGfdyralarelqaEVPSLRhvlVVGDAgEFTSLDA----LLAA 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1672 PDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEH---IGIVRYLswAAASYPTAGRRgTLAHSSVGFDLTMS---ALFEP 1745
Cdd:COG1021   173 PADLSEPRPDPDDVAFFQLSGGTTGLPKLIPRTHddyLYSVRAS--AEICGLDADTV-YLAALPAAHNFPLSspgVLGVL 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1746 LVSGRGVtlmpadatladLAEELSGPLAYDYIRltpshlRH------LVghwtgqelPPAARGWV--------------- 1804
Cdd:COG1021   250 YAGGTVV-----------LAPDPSPDTAFPLIE------RErvtvtaLV--------PPLALLWLdaaersrydlsslrv 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1805 --VGGETLDPALVKQLLEL-------------------RPDAevinhygPTETVIGRVVHPVREAGE-LAVDsplPLGRP 1862
Cdd:COG1021   305 lqVGGAKLSPELARRVRPAlgctlqqvfgmaeglvnytRLDD-------PEEVILTTQGRPISPDDEvRIVD---EDGNP 374

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1863 lgetrlqvldawleaVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepGarMYRTGDLVRWRGDGLLDFVGRVD 1942
Cdd:COG1021   375 ---------------VPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPD-----G--FYRTGDLVRRTPDGYLVVEGRAK 432

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1943 DQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQQL---VGWFIPAEDHPPvTVSALRRFCAEQ-LPEFMVPNQWVAL 2017
Cdd:COG1021   433 DQINRGGEKIAAEEVENLLLAHPAVHDaAVVAMPDEYLgerSCAFVVPRGEPL-TLAELRRFLRERgLAAFKLPDRLEFV 511

                         570
                  ....*....|....*.
gi 653678460 2018 DAFPLTPHGKVNHRAL 2033
Cdd:COG1021   512 DALPLTAVGKIDKKAL 527
Cyc_NRPS cd19535
Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 81-370 6.71e-30

Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Cyc (heterocyclization) domains catalyze two separate reactions in the creation of heterocyclized peptide products in nonribosomal peptide synthesis: amide bond formation followed by intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and a carbonyl carbon on the peptide backbone to form a thiazoline, oxazoline, or methyloxazoline ring. Cyc-domains are homologous to standard NRPS Condensation (C) domains. C-domains typically have a conserved HHxxxD motif at the active site; Cyc-domains have an alternative, conserved DxxxxD active site motif, mutation of the aspartate residues in this motif can abolish or diminish condensation activity. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and Cyc-domains. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380458 [Multi-domain]  Cd Length: 423  Bit Score: 125.29  E-value: 6.71e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   81 LDVDALRAALDAVTARHEALRTVFrlGPDGEpvQQTAPAAP-VALPVIDVTEADLPQALRTAAE-------QPFDLERGP 152
Cdd:cd19535    37 LDPDRLERAWNKLIARHPMLRAVF--LDDGT--QQILPEVPwYGITVHDLRGLSEEEAEAALEElrerlshRVLDVERGP 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  153 LLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG---ELPAgvAAPAFRD-VAAWQHGRLAAGELDdqLRY 228
Cdd:cd19535   113 LFDIRLSLLPEGRTRLHLSIDLLVADALSLQILLRELAALYEDpgePLPP--LELSFRDyLLAEQALRETAYERA--RAY 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  229 WRQRLAGL---PDLEIpgdRQRPADRDwRAHSVRRE--LPAATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFA 303
Cdd:cd19535   189 WQERLPTLppaPQLPL---AKDPEEIK-EPRFTRREhrLSAEQWQRLKERARQHGVTPSMVLLTAYAEVLARWSGQPRFL 264

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460  304 VGVPVAGR--GRTDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDFAHPQIPFDRLVRELRR 370
Cdd:cd19535   265 LNLTLFNRlpLHPDVNDVVGDFTSLLLLEVDGSEGQSFLERARRLQQQLWEDLDHSSYSGVVVVRRLLR 333
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
 1548-2033 1.12e-29

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 124.88  E-value: 1.12e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGGyfipvdpgypmarltr 1627
Cdd:cd05919     2 TAFYAADRSVTYGQLHDGANRLGSALRN--LG---VSSGDRVLLLMLDSPELVQLFLGCLARGA---------------- 60

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1628 iadtvtpalvltrtgqggclpgaevfgdVPVVA--LDRVADRLTAMPDQRPE-VTVDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:cd05919    61 ----------------------------IAVVInpLLHPDDYAYIARDCEARlVVTSADDIAYLLYSSGTTGPPKGVMHA 112

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1705 H---IGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFePLVSGRGVTLMP----ADATLADLAEE-----LSGPL 1772
Cdd:cd05919   113 HrdpLLFADAMAREALGLTPGDRVFSSAKMFFGYGLGNSLWF-PLAVGASAVLNPgwptAERVLATLARFrptvlYGVPT 191

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 AYDYIRLTPSHLRHLVGhwtgqelppAARGWVVGGETLDPALVKQLLELRpDAEVINHYGPTETVIgrvVHPVREAGELA 1852
Cdd:cd05919   192 FYANLLDSCAGSPDALR---------SLRLCVSAGEALPRGLGERWMEHF-GGPILDGIGATEVGH---IFLSNRPGAWR 258

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1853 VDSplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpAGEpgarMYRTGDLVRWRGD 1932
Cdd:cd05919   259 LGS---TGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATF----NGG----WYRTGDKFCRDAD 327

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1933 GLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ----QLVGWFIPAEDHPP--VTVSALRRFCAEQL 2005
Cdd:cd05919   328 GWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVaEAAVVAVPEStglsRLTAFVVLKSPAAPqeSLARDIHRHLLERL 407

                         490       500
                  ....*....|....*....|....*...
gi 653678460 2006 PEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05919   408 SAHKVPRRIAFVDELPRTATGKLQRFKL 435
PRK05691 PRK05691
peptide synthase; Validated
 45-331 1.55e-29

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 129.90  E-value: 1.55e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   45 GPVPLAPGQAgiWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVAL 124
Cdd:PRK05691 2790 GASGLTPIQH--WFFDSPVPQPQHWNQALLLEPRQALDPALLEQALQALVEHHDALRLRFSQADGRWQAEYRAVTAQELL 2867

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  125 PVIDVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAY-AGELPAGVAA 203
Cdd:PRK05691 2868 WQVTVADFAECAALFADAQRSLDLQQGPLLRALLVDGPQGQQRLLLAIHHLVVDGVSWRVLLEDLQALYrQLSAGAEPAL 2947

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  204 PA----FRDVAAWQHGRLAAGELDDQLRYWRQRLAGlPDLEIPGDRQRPADRDWRAHSVRREL-PAATEAAVRRLAAAHD 278
Cdd:PRK05691 2948 PAktsaFRDWAARLQAYAGSESLREELGWWQAQLGG-PRAELPCDRPQGGNLNRHAQTVSVRLdAERTRQLLQQAPAAYR 3026

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460  279 TTPFTVLLAAYGTFLHRLTGQDdfAVGVPVAGRGR------TDLESVVGLFAGMVPVRL 331
Cdd:PRK05691 3027 TQVNDLLLTALARVLCRWSGQP--SVLVQLEGHGRealfddIDLTRSVGWFTSAYPLRL 3083
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
 516-977 1.77e-29

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 123.61  E-value: 1.77e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLvvtd 595
Cdd:cd05912     1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL---- 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  596 aatadrlgpDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDR 675
Cdd:cd05912    77 ---------DDI---------------------------ATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGLTEDDN 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATTTVsFDIAGLELYlpLRS---GAGVVLADADTARNPAALIDlagRHRVTLAQATPTLWQALVPELSGPALAGIR-V 751
Cdd:cd05912   121 WLCALPL-FHISGLSIL--MRSviyGMTVYLVDKFDAEQVLHLIN---SGKVTIISVVPTMLQRLLEILGEGYPNNLRcI 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  752 LVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIgvlGELHIA 831
Cdd:cd05912   195 LLGGGPAPKPLLEQCKEKGIPVYQSYGMTETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYEV---GEILLK 271

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 GEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR 911
Cdd:cd05912   272 GPNVTKGYLNRPDATEESFENGWF--------KTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKE 343

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  912 AAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05912   344 AGVVGIPDDKWGQVPVAFVVSERPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
 1557-2033 2.42e-29

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 123.60  E-value: 2.42e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY-PMARLTRIADTVTPA 1635
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLGLRKGDR-----VAVLLPRVPELWAVILAVIKLGAVYVPLTTLLgPKDIEYRLEAAGAKA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1636 LVltrtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtVDPRGLAYSIFTSGSTGQPKGVAVEHigivrylSWA 1715
Cdd:cd05972    76 IV------------------------------------------TDAEDPALIYFTSGTTGLPKGVLHTH-------SYP 106

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1716 AASYPTAGRRGTLAHSSVGFDL--------TMSALFEPLVSGRGVTLMPADATLADLAEELSGPLAYDYIRLTPSHLRHL 1787
Cdd:cd05972   107 LGHIPTAAYWLGLRPDDIHWNIadpgwakgAWSSFFGPWLLGATVFVYEGPRFDAERILELLERYGVTSFCGPPTAYRML 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1788 VGHWTGQELPPAARGWVVGGETLDPALVKQLLElRPDAEVINHYGPTETVIGRVVHPVREAgelavdSPLPLGRPLGETR 1867
Cdd:cd05972   187 IKQDLSSYKFSHLRLVVSAGEPLNPEVIEWWRA-ATGLPIRDGYGQTETGLTVGNFPDMPV------KPGSMGRPTPGYD 259

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1868 LQVLDAWLEAVPVGAVGELFI--GGDGIARGYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQV 1945
Cdd:cd05972   260 VAIIDDDGRELPPGEEGDIAIklPPPGLFLGYVGDPEKTEASIRGD--------YYLTGDRAYRDEDGYFWFVGRADDII 331

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1946 KLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ---QLVGWFI---PAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALD 2018
Cdd:cd05972   332 KSSGYRIGPFEVESALLEHPAVaEAAVVGSPDPvrgEVVKAFVvltSGYEPSEELAEELQGHVKKVLAPYKYPREIEFVE 411

                         490
                  ....*....|....*
gi 653678460 2019 AFPLTPHGKVNHRAL 2033
Cdd:cd05972   412 ELPKTISGKIRRVEL 426
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
 505-971 2.96e-29

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 124.33  E-value: 2.96e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:cd12118    18 PDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFIL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATAdrlgpdditglvvLEETDTGGYPatEPPAVPAGHSAYVI---YTSGSTGRPKGVVIT-RAAldnF 660
Cdd:cd12118    98 RHSEAKVLFVDREFE-------------YEDLLAEGDP--DFEWIPPADEWDPIalnYTSGTTGRPKGVVYHhRGA---Y 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  661 LAAMAerfpltaedrvlatTTVSFDIAGLELYL---PL--------------RSGAGVVLADADtarnPAALIDLAGRHR 723
Cdd:cd12118   160 LNALA--------------NILEWEMKQHPVYLwtlPMfhcngwcfpwtvaaVGGTNVCLRKVD----AKAIYDLIEKHK 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  724 VTLAQATPTLWQALV---PELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETT------IWS---TSGPT 791
Cdd:cd12118   222 VTHFCGAPTVLNMLAnapPSDARPLPHRVHVMTAGAPPPAAVLAKMEELGFDVTHVYGLTETYgpatvcAWKpewDELPT 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 SEDSIRRGSIGVP-IDNTQVYVLDANLH---PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpfgppgtR--MYRT 865
Cdd:cd12118   302 EERARLKARQGVRyVGLEEVDVLDPETMkpvPRDGKTIGEIVFRGNIVMKGYLKNPEATAEAF----------RggWFHS 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  866 GDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRT 943
Cdd:cd12118   372 GDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVElkEGAKVTEEEIIA 451

                         490       500
                  ....*....|....*....|....*...
gi 653678460  944 ELSRTLPDYMIPAVIVpVPDFPTTPNGK 971
Cdd:cd12118   452 FCREHLAGFMVPKTVV-FGELPKTSTGK 478
Thioesterase pfam00975
Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of ...
 2157-2311 4.40e-29

Thioesterase domain; Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.


Pssm-ID: 395776 [Multi-domain]  Cd Length: 223  Bit Score: 117.49  E-value: 4.40e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2157 RPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGTIAF 2236
Cdd:pfam00975    1 RPLFCFPPAGGSASSFRSLARRLPPPAEVLAVQYPGRGRGEPPLNSIEALADEYAEALRQIQPEGPYALFGHSMGGMLAF 80

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  2237 EMARQLDRLGEPVQ-LILLEPTLPEVARTIDLHRPARDSYLrgaDLAERILRLPAGDPEGDRLRAELTRLLEDAGY 2311
Cdd:pfam00975   81 EVARRLERQGEAVRsLFLSDASAPHTVRYEASRAPDDDEVV---AEFTDEGGTPEELLEDEELLSMLLPALRADYR 153
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
 1529-1988 6.66e-29

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 123.88  E-value: 6.66e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1529 DDRTVHQLVEAQADRTPGWTAL---RTGdRSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLA 1605
Cdd:cd05904     3 TDLPLDSVSFLFASAHPSRPALidaATG-RALTYAELERRVRRLAAGLAKRGGRKGDV-----VLLLSPNSIEFPVAFLA 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1606 VLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAevfgDVPVVALDRVADRLTAM--------PDQRPE 1677
Cdd:cd05904    77 VLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAELAEKLASL----ALPVVLLDSAEFDSLSFsdllfeadEAEPPV 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1678 VTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVrylswAAASYPTAGRRGTLAHSSV-----------GFDLTMSALfepL 1746
Cdd:cd05904   153 VVIKQDDVAALLYSSGTTGRSKGVMLTHRNLI-----AMVAQFVAGEGSNSDSEDVflcvlpmfhiyGLSSFALGL---L 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1747 VSGRGVTLMPAdatlADLAEELSGPLAYD--YIRLTPSHLRHLVGHWTGQELP-PAARGWVVGGETLDPALVKQLLELRP 1823
Cdd:cd05904   225 RLGATVVVMPR----FDLEELLAAIERYKvtHLPVVPPIVLALVKSPIVDKYDlSSLRQIMSGAAPLGKELIEAFRAKFP 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1824 DAEVINHYGPTETVIGRVVHPVREAGELAVDSplpLGRPLGETRLQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPA 1902
Cdd:cd05904   301 NVDLGQGYGMTESTGVVAMCFAPEKDRAKYGS---VGRLVPNVEAKIVDpETGESLPPNQTGELWIRGPSIMKGYLNNPE 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1903 LTAEKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqqlvgw 1982
Cdd:cd05904   378 ATAATIDKE-------GWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAV---------- 440


                  ....*.
gi 653678460 1983 fIPAED 1988
Cdd:cd05904   441 -IPYPD 445
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 516-977 8.33e-29

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 122.57  E-value: 8.33e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVtd 595
Cdd:cd05910     2 RLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAFI-- 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  596 aatadrlgpdditglvvleetdtgGYPATEPPAVpaghsayVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDR 675
Cdd:cd05910    80 ------------------------GIPKADEPAA-------ILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEV 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATttvsFDIAGleLYLPLRsGAGVVLADADTAR----NPAALIDLAGRHRVTLAQATPTLWQALVP--ELSGPALAGI 749
Cdd:cd05910   129 DLAT----FPLFA--LFGPAL-GLTSVIPDMDPTRparaDPQKLVGAIRQYGVSIVFGSPALLERVARycAQHGITLPSL 201

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 R-VLVGGEALPAELARRLGDA---GAEVTNMYGPTET----------TIWSTSGPTSEDsirRGS-IGVPIDNTQVYVLD 814
Cdd:cd05910   202 RrVLSAGAPVPIALAARLRKMlsdEAEILTPYGATEAlpvssigsreLLATTTAATSGG---AGTcVGRPIPGVRVRIIE 278

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  815 ANLHP---------APIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgPPGTRMYRTGDLVRWSAAGDLEYLGRTDH 885
Cdd:cd05910   279 IDDEPiaewddtleLPRGEIGEITVTGPTVTPTYVNRPVATALAKIDD---NSEGFWHRMGDLGYLDDEGRLWFCGRKAH 355

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  886 QVKLRGFRIELGEIEtTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDA--LRTELSRTLPDYMIPAVIVPV-- 961
Cdd:cd05910   356 RVITTGGTLYTEPVE-RVFNTHPGVRRSALVGVGKPGCQLPVLCVEPLPGTITPRarLEQELRALAKDYPHTQRIGRFli 434

                         490
                  ....*....|....*....
gi 653678460  962 -PDFPTTP--NGKLDRAAL 977
Cdd:cd05910   435 hPSFPVDIrhNAKIFREKL 453
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
 487-974 8.51e-29

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 124.34  E-value: 8.51e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  487 PLPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGA 566
Cdd:PRK05605   28 DYGDTTLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGA 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  567 AYLPIDPDFPVERIAYLLSD--ARpVLVVTD--AATADRLGPDdiTGL---VVLEETD---------------------- 617
Cdd:PRK05605  108 VVVEHNPLYTAHELEHPFEDhgAR-VAIVWDkvAPTVERLRRT--TPLetiVSVNMIAampllqrlalrlpipalrkara 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  618 --TGGYPATEP------------------PAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAED--R 675
Cdd:PRK05605  185 alTGPAPGTVPwetlvdaaiggdgsdvshPRPTPDDVALILYTSGTTGKPKGAQLTHRNLFANAAQGKAWVPGLGDGpeR 264

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATTTVsFDIAGLELYLPLRS--GAGVVLAdadtarnPAALIDL----AGRHRVTLAQATPTLWQALV--PELSGPALA 747
Cdd:PRK05605  265 VLAALPM-FHAYGLTLCLTLAVsiGGELVLL-------PAPDIDLildaMKKHPPTWLPGVPPLYEKIAeaAEERGVDLS 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  748 GIRV-LVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTQVYVLDANlHPA---PI 822
Cdd:PRK05605  337 GVRNaFSGAMALPVSTVELWEKLtGGLLVEGYGLTETSPIIVGNPMSDDR-RPGYVGVPFPDTEVRIVDPE-DPDetmPD 414

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  823 GVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETT 902
Cdd:PRK05605  415 GEEGELLVRGPQVFKGYWNRPEETAKSFLDG--------WFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEV 486

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  903 LINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVA--PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:PRK05605  487 LREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAAldPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRR 560
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
 1552-2028 8.87e-29

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 124.61  E-value: 8.87e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1552 TGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY-PMARLTRIAD 1630
Cdd:cd17634    80 SQSRTISYRELHREVCRFA-----GTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFaPEAVAGRIID 154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1631 TVTPALVltrTGQGGCLPG------------AEVFGDVP--VVALDRVA--------------DRLTAMPDQRPEVTVDP 1682
Cdd:cd17634   155 SSSRLLI---TADGGVRAGrsvplkknvddaLNPNVTSVehVIVLKRTGsdidwqegrdlwwrDLIAKASPEHQPEAMNA 231

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1683 RGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAAS-YPTAGRRGTLAHSSVGFDLTMSAL-FEPLVSG------RGVTL 1754
Cdd:cd17634   232 EDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYvFDYGPGDIYWCTADVGWVTGHSYLlYGPLACGattllyEGVPN 311

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MPADATLADLAEE------LSGPLAY--------DYI-RLTPSHLRHLVGhwTGQELPPAARGW---VVGGETldpalvk 1816
Cdd:cd17634   312 WPTPARMWQVVDKhgvnilYTAPTAIralmaagdDAIeGTDRSSLRILGS--VGEPINPEAYEWywkKIGKEK------- 382

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1817 qllelRPdaeVINHYGPTETViGRVVHPVREAGELAVDSPLplgRPLGETRLQVLDAWLEAVPVGAVGELFIGGD--GIA 1894
Cdd:cd17634   383 -----CP---VVDTWWQTETG-GFMITPLPGAIELKAGSAT---RPVFGVQPAVVDNEGHPQPGGTEGNLVITDPwpGQT 450

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1895 RGYLGRPALTAEKFLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVL 1973
Cdd:cd17634   451 RTLFGDHERFEQTYFSTFKG-----MYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVaEAAVVG 525

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1974 VRD----QQLVGWFI--PAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd17634   526 IPHaikgQAPYAYVVlnHGVEPSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
PRK09274 PRK09274
peptide synthase; Provisional
 485-977 3.33e-28

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 122.31  E-value: 3.33e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  485 ARPLPAASlrerfqqwcRRTPGAVAVIEGDT----------TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADL 554
Cdd:PRK09274    9 ARHLPRAA---------QERPDQLAVAVPGGrgadgklaydELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEF 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  555 LVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP--VLVVTDAATADRL---GPDDITGLVVLEETD-TGGYPATEPPA 628
Cdd:PRK09274   80 FALTFALFKAGAVPVLVDPGMGIKNLKQCLAEAQPdaFIGIPKAHLARRLfgwGKPSVRRLVTVGGRLlWGGTTLATLLR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  629 VPAGHS-----------AYVIYTSGSTGRPKGVVITRaalDNFLA---AMAERFPLTAEDRVLATttvsfdiaglelyLP 694
Cdd:PRK09274  160 DGAAAPfpmadlapddmAAILFTSGSTGTPKGVVYTH---GMFEAqieALREDYGIEPGEIDLPT-------------FP 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  695 LRS------GAGVVLADADTAR----NPAALIDLAGRHRVTLAQATPTLWQALVP--ELSGPALAGI-RVLVGGEALPAE 761
Cdd:PRK09274  224 LFAlfgpalGMTSVIPDMDPTRpatvDPAKLFAAIERYGVTNLFGSPALLERLGRygEANGIKLPSLrRVISAGAPVPIA 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  762 LARRLGD---AGAEVTNMYGPTE----TTIWSTS--GPTSEDSIRRGSI--GVPIDNTQVYVLD---------ANLHPAP 821
Cdd:PRK09274  304 VIERFRAmlpPDAEILTPYGATEalpiSSIESREilFATRAATDNGAGIcvGRPVDGVEVRIIAisdapipewDDALRLA 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  822 IGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGppGTRmYRTGDLVRWSAAGDLEYLGRTDHQVKLRG---FRIELGE 898
Cdd:PRK09274  384 TGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGQG--DVW-HRMGDLGYLDAQGRLWFCGRKAHRVETAGgtlYTIPCER 460

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  899 IettlINAGP-VRRAAAV-VREdrPGDLR--LVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPV---PDFPTTP--N 969
Cdd:PRK09274  461 I----FNTHPgVKRSALVgVGV--PGAQRpvLCVELEPGVACSKSALYQELRALAAAHPHTAGIERFlihPSFPVDIrhN 534


                  ....*...
gi 653678460  970 GKLDRAAL 977
Cdd:PRK09274  535 AKIFREKL 542
beta-lac_NRPS cd19547
Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis ...
 48-379 4.80e-28

Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis NocB which exhibits an unusual cyclization to form beta-lactam rings in pro-nocardicin G synthesis; Nocardia uniformis NRPS NocB acts centrally in the biosynthesis of the nocardicin monocyclic beta-lactam antibiotics. Along with another NRPS NocA, it mediates an unusual cyclization to form beta-lactam rings in the synthesis of the beta-lactam-containing pentapeptide pro-nocardicin G. This small subfamily is related to DCL-type Condensation (C) domains, which catalyze condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; domains belonging to this subfamily have an HHHxxxD motif at the active site.


Pssm-ID: 380469 [Multi-domain]  Cd Length: 422  Bit Score: 119.72  E-value: 4.80e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPVI 127
Cdd:cd19547     3 PLAPMQEGMLFRGLFWPDSDAYFNQNVLELVGGTDEDVLREAWRRVADRYEILRTGFTWRDRAEPLQYVRDDLAPPWALL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  128 DVTEAD-------LPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYA----GE 196
Cdd:cd19547    83 DWSGEDpdrraelLERLLADDRAAGLSLADCPLYRLTLVRLGGGRHYLLWSHHHILLDGWCLSLIWGDVFRVYEelahGR 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  197 LPAGVAAPAFRDVAAWQHGRLAAGelDDQLRYWRQRLAglpDLEIPGDRQRPADRDWRAHSVRRELPAATEAAVRRLAAA 276
Cdd:cd19547   163 EPQLSPCRPYRDYVRWIRARTAQS--EESERFWREYLR---DLTPSPFSTAPADREGEFDTVVHEFPEQLTRLVNEAARG 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  277 HDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGRGR--TDLESVVGLFAGMVPVRLDLTGRPSFDEVLSRTVAGSRNDF 354
Cdd:cd19547   238 YGVTTNAISQAAWSMLLALQTGARDVVHGLTIAGRPPelEGSEHMVGIFINTIPLRIRLDPDQTVTGLLETIHRDLATTA 317

                         330       340
                  ....*....|....*....|....*
gi 653678460  355 AHPQIPFDRLVRELRRDRIAGSQLL 379
Cdd:cd19547   318 AHGHVPLAQIKSWASGERLSGGRVF 342
PRK12316 PRK12316
peptide synthase; Provisional
 31-331 7.08e-28

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 124.68  E-value: 7.08e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   31 QGPAhggglpvqpPGPVPLAPGQAgiWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRlGPDG 110
Cdd:PRK12316 1094 QGPA---------SGEVALAPVQR--WFFEQAIPQRQHWNQSLLLQARQPLDPDRLGRALERLVAHHDALRLRFR-EEDG 1161

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  111 EpvQQTAPAAPVALPVI---DVTEADLPQALRTAAEQPFDLERGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLA 187
Cdd:PRK12316 1162 G--WQQAYAAPQAGEVLwqrQAASEEELLALCEEAQRSLDLEQGPLLRALLVDMADGSQRLLLVIHHLVVDGVSWRILLE 1239

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  188 DLARAYAGELPA-GVAAPAFRDVAAWQHGRlaAGELDDQLRYWRQRLAGLPDlEIPGDRQRPADRDWRAHSVRRELPAAT 266
Cdd:PRK12316 1240 DLQRAYADLDADlPARTSSYQAWARRLHEH--AGARAEELDYWQAQLEDAPH-ELPCENPDGALENRHERKLELRLDAER 1316

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460  267 EAAVRRLA-AAHDTTPFTVLLAAYGTFLHRLTGQDdfAVGVPVAGRGR------TDLESVVGLFAGMVPVRL 331
Cdd:PRK12316 1317 TRQLLQEApAAYRTQVNDLLLTALARVTCRWSGQA--SVLVQLEGHGRedlfedIDLSRTVGWFTSLFPVRL 1386
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
 1531-2033 1.27e-27

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 119.54  E-value: 1.27e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1531 RTVHQLVEAQADRTPGWTALRTGDRS--LTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLK 1608
Cdd:cd05923     1 QTVFEMLRRAASRAPDACAIADPARGlrLTYSELRARIEAVAARLH--ARG---LRPGQRVAVVLPNSVEAVIALLALHR 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1609 AGGYFIPVDPgypMARLTRIADTVTPALVLTRTGQGGCLPGAEVF-GDVPVVAL-DRVADR-------LTAMPDQRPEVT 1679
Cdd:cd05923    76 LGAVPALINP---RLKAAELAELIERGEMTAAVIAVDAQVMDAIFqSGVRVLALsDLVGLGepesagpLIEDPPREPEQP 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1680 vdprglAYSIFTSGSTGQPKGVAVEHIGIV-RYLSWAAASYPTAGRRGTLA-----HSSVGFdltmSALFEPLVSGRGVT 1753
Cdd:cd05923   153 ------AFVFYTSGTTGLPKGAVIPQRAAEsRVLFMSTQAGLRHGRHNVVLglmplYHVIGF----FAVLVAALALDGTY 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1754 LMPADATLADlAEELSGPLAYDYIRLTPSHLRHLVGHWTGQELP-PAARGWVVGGETLDPALVKQLLELRPdAEVINHYG 1832
Cdd:cd05923   223 VVVEEFDPAD-ALKLIEQERVTSLFATPTHLDALAAAAEFAGLKlSSLRHVTFAGATMPDAVLERVNQHLP-GEKVNIYG 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1833 PTETVIGRVVHPVREAGELavdsplplgRPLGETRLQ---VLDAWLEAVPVGAVGELFI--GGDGIARGYLGRPALTAEK 1907
Cdd:cd05923   301 TTEAMNSLYMRDARTGTEM---------RPGFFSEVRivrIGGSPDEALANGEEGELIVaaAADAAFTGYLNQPEATAKK 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1908 fLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQ---QLVGWF 1983
Cdd:cd05923   372 -LQD-------GWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIgVADErwgQSVTAC 443

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1984 IPAEDHPPvTVSALRRFC-AEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05923   444 VVPREGTL-SADELDQFCrASELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
C_NRPS-like cd19537
Condensation family domain with an atypical active site motif; Condensation (C) domains of ...
 1082-1494 1.41e-27

Condensation family domain with an atypical active site motif; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. Members of this subfamily typically have a non-canonical conserved SHXXXDX(14)Y motif. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380460 [Multi-domain]  Cd Length: 395  Bit Score: 117.67  E-value: 1.41e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGM-------PVQVADP 1154
Cdd:cd19537     1 DTALSPIEREWWHKYQLSTGTSSFNVSFACRLSGDVDRDRLASAWNTVLARHRILRSRYVPRDGGLrrsysssPPRVQRV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1155 agGGAALTERDAAPgtdlaqlllaeaalgFTLATEHPLRAVLwrldEREHvLLLTAHHVAVDAWSMDLIQRDLTELYAAD 1234
Cdd:cd19537    81 --DTLDVWKEINRP---------------FDLEREDPIRVFI----SPDT-LLVVMSHIICDLTTLQLLLREVSAAYNGK 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1235 TghrepeLDEPALAQADYAVWQRQSAQRgryaaELDFWRTELTGAVATevagDLPRPATPGGGG-GAVEFALAPRQIQGI 1313
Cdd:cd19537   139 L------LPPVRREYLDSTAWSRPASPE-----DLDFWSEYLSGLPLL----NLPRRTSSKSYRgTSRVFQLPGSLYRSL 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1314 RELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLR--GDLSGAPTFHELLRRTHA 1391
Cdd:cd19537   204 LQFSTSSGITLHQLALAAVALALQDLSDRTDIVLGAPYLNRTSEEDMETVGLFLEPLPIRirFPSSSDASAADFLRAVRR 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1392 RTADALAHQeLPF----EHLVADRGAGNGPLFRIMYSFSSQEpAGRSAGDVD-LEPVPVPVTTCKFDLVL--TVVDGGS- 1463
Cdd:cd19537   284 SSQAALAHA-IPWhqllEHLGLPPDSPNHPLFDVMVTFHDDR-GVSLALPIPgVEPLYTWAEGAKFPLMFefTALSDDSl 361

                         410       420       430
                  ....*....|....*....|....*....|.
gi 653678460 1464 TLEgvLEYADDLYLPGTAERLVTSLTNLLAA 1494
Cdd:cd19537   362 LLR--LEYDTDCFSEEEIDRIESLILAALEL 390
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
 515-973 4.33e-27

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 117.82  E-value: 4.33e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLARLLmERGAGAETFVAVLLPRSADLLVTLLAVIKAGaaYLPIDPDFP--VERIAYLLSDARPVLV 592
Cdd:cd05909     6 TSLTYRKLLTGAIALARKL-AKMTKEGENVGVMLPPSAGGALANFALALSG--KVPVMLNYTagLRELRACIKLAGIKTV 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  593 VTDAATADRLGPDDIT------GLVVLEETDTG---------GYPATEPPA----------VPAGHSAYVIYTSGSTGRP 647
Cdd:cd05909    83 LTSKQFIEKLKLHHLFdveydaRIVYLEDLRAKiskadkckaFLAGKFPPKwllrifgvapVQPDDPAVILFTSGSEGLP 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  648 KGVVITRAALDNFLAAMAERFPLTAEDRVLATTTV--SFDIAGlELYLPLRSGAGVVLA-DADTARNPAALIDLAGrhrV 724
Cdd:cd05909   163 KGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFfhSFGLTG-CLWLPLLSGIKVVFHpNPLDYKKIPELIYDKK---A 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  725 TLAQATPTLWQALVPELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETT-IWSTSGPTSEDsiRRGSI 801
Cdd:cd05909   239 TILLGTPTFLRGYARAAHPEDFSSLRlVVAGAEKLKDTLRQEFQEKfGIRILEGYGTTECSpVISVNTPQSPN--KEGTV 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  802 GVPIDNTQVYVLD-ANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpfgppGTRMYRTGDLVRWSAAGDLEYL 880
Cdd:cd05909   317 GRPLPGMEVKIVSvETHEEVPIGEGGLLLVRGPNVMLGYLNEPELTSFAF--------GDGWYDTGDIGKIDGEGFLTIT 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  881 GRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV--VREDRPGDLRLVAYVIPDGpvAPDALRTELSRT-LPDYMIPAV 957
Cdd:cd05909   389 GRLSRFAKIAGEMVSLEAIEDILSEILPEDNEVAVvsVPDGRKGEKIVLLTTTTDT--DPSSLNDILKNAgISNLAKPSY 466

                         490
                  ....*....|....*.
gi 653678460  958 IVPVPDFPTTPNGKLD 973
Cdd:cd05909   467 IHQVEEIPLLGTGKPD 482
menE TIGR01923
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ...
 1558-2033 5.01e-27

O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]


Pssm-ID: 162605 [Multi-domain]  Cd Length: 436  Bit Score: 116.78  E-value: 5.01e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1558 TFAGLDAQANRLAHALHtgalgAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALV 1637
Cdd:TIGR01923    1 TWQDLDCEAAHLAKALK-----AQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLL 75

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1638 LTrtgqggclpgAEVFGDVPVVALDrvADRLTAmPDQRP-EVTVDPRG--LAYSIFTSGSTGQPKGVAVEH--------- 1705
Cdd:TIGR01923   76 LT----------DSLLEEKDFQADS--LDRIEA-AGRYEtSLSASFNMdqIATLMFTSGTTGKPKAVPHTFrnhyasavg 142

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1706 ----IGIVRYLSWAAaSYPtagrrgtLAHSSvgfdlTMSALFEPLVSGRGVTLMPADAtlaDLAEELSGPlAYDYIRLTP 1781
Cdd:TIGR01923  143 skenLGFTEDDNWLL-SLP-------LYHIS-----GLSILFRWLIEGATLRIVDKFN---QLLEMIANE-RVTHISLVP 205

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1782 SHLRHLVGHWTGQELppaARGWVVGGETLDPALVKQLLELRPDaeVINHYGPTETVIGRVVHPVR-EAGELAVDSPLPlG 1860
Cdd:TIGR01923  206 TQLNRLLDEGGHNEN---LRKILLGGSAIPAPLIEEAQQYGLP--IYLSYGMTETCSQVTTATPEmLHARPDVGRPLA-G 279

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1861 RplgETRLQVLDAwleavpvGAVGELFIGGDGIARGYLGRPALTaekflpdPAGEPGArMYRTGDLVRWRGDGLLDFVGR 1940
Cdd:TIGR01923  280 R---EIKIKVDNK-------EGHGEIMVKGANLMKGYLYQGELT-------PAFEQQG-WFNTGDIGELDGEGFLYVLGR 341

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  1941 VDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqqlvgwfIPAED-------------HPPVTVSALRRFCAEQLPE 2007
Cdd:TIGR01923  342 RDDLIISGGENIYPEEIETVLYQHPGIQEAVV-----------VPKPDaewgqvpvayivsESDISQAKLIAYLTEKLAK 410

                          490       500
                   ....*....|....*....|....*.
gi 653678460  2008 FMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:TIGR01923  411 YKVPIAFEKLDELPYNASGKILRNQL 436
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
 1541-2039 5.80e-27

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 118.50  E-value: 5.80e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALR------TGDRSLTFAGLDAQANRLAHALHtgALGAPPvgpeTPVLLLLDRSPELVVAMLAVLKAGGYFI 1614
Cdd:cd05931     3 AAARPDRPAYTflddegGREETLTYAELDRRARAIAARLQ--AVGKPG----DRVLLLAPPGLDFVAAFLGCLYAGAIAV 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 PV---DPGYPMARLTRIADTVTPALVLTRTGQGGCLPG----AEVFGDVPVVALDRVADRLtamPDQRPEVTVDPRGLAY 1687
Cdd:cd05931    77 PLpppTPGRHAERLAAILADAGPRVVLTTAAALAAVRAfaasRPAAGTPRLLVVDLLPDTS---AADWPPPSPDPDDIAY 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1688 SIFTSGSTGQPKGVAVEHIGIVRYLSWAAASY-PTAGRRGT----LAHssvgfDLTM-SALFEPLVSGRGVTLMPADATL 1761
Cdd:cd05931   154 LQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYgLDPGDVVVswlpLYH-----DMGLiGGLLTPLYSGGPSVLMSPAAFL 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1762 ADLA---EELS--------GP-LAYDYI--RLTPSHLRHL-VGHWtgqelppaaRGWVVGGETLDPALVKQLLE------ 1820
Cdd:cd05931   229 RRPLrwlRLISryratisaAPnFAYDLCvrRVRDEDLEGLdLSSW---------RVALNGAEPVRPATLRRFAEafapfg 299

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1821 LRPDAeVINHYGPTETVI---------GRVVHPVR----EAGELAVDSP-------LPLGRPLGETRLQVLDA-WLEAVP 1879
Cdd:cd05931   300 FRPEA-FRPSYGLAEATLfvsggppgtGPVVLRVDrdalAGRAVAVAADdpaarelVSCGRPLPDQEVRIVDPeTGRELP 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1880 VGAVGELFIGGDGIARGYLGRPALTAEKFLPDpAGEPGARMYRTGDLvRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEA 1959
Cdd:cd05931   379 DGEVGEIWVRGPSVASGYWGRPEATAETFGAL-AATDEGGWLRTGDL-GFLHDGELYITGRLKDLIIVRGRNHYPQDIEA 456

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1960 RMAEHPGVEQ----AVVLVRDQQLVGWFIPAEDHPPVTVSALRRFCAEqlpefMVpnQWVAlDAFPLTPHGK--VNHRAL 2033
Cdd:cd05931   457 TAEEAHPALRpgcvAAFSVPDDGEERLVVVAEVERGADPADLAAIAAA-----IR--AAVA-REHGVAPADVvlVRPGSI 528


                  ....*.
gi 653678460 2034 PGPTSG 2039
Cdd:cd05931   529 PRTSSG 534
FUM14_C_NRPS-like cd19545
Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond ...
 1084-1499 6.82e-27

Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond forming Fusarium verticillioides FUM14 protein; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) typically catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. However, some C-domains have ester-bond forming activity. This subfamily includes Fusarium verticillioides FUM14 (also known as NRPS8), a bi-domain protein with an ester-bond forming NRPS C-domain, which catalyzes linkages between an aminoacyl/peptidyl-PCP donor and a hydroxyl-containing acceptor. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. FUM14 has an altered active site motif DHTHCD instead of the typical HHxxxD motif seen in other subfamily members. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380467 [Multi-domain]  Cd Length: 395  Bit Score: 115.47  E-value: 6.82e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESiwLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVAdpagggaalte 1163
Cdd:cd19545     3 PCTPLQEG--LMALTARQPGAYVGQRVFELPPDIDLARLQAAWEQVVQANPILRTRIVQSDSGGLLQVV----------- 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1164 RDAAP-----GTDLAQLLLAEAALGFTLATehPL-RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAadtgh 1237
Cdd:cd19545    70 VKESPiswteSTSLDEYLEEDRAAPMGLGG--PLvRLALVEDPDTERYFVWTIHHALYDGWSLPLILRQVLAAYQ----- 142

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1238 repelDEPALAQADYAVWQRQSAQRGRYAAElDFWRTELTGAVATEVagdlprPATPGGGGGAVEFALAPRQIQgIRELA 1317
Cdd:cd19545   143 -----GEPVPQPPPFSRFVKYLRQLDDEAAA-EFWRSYLAGLDPAVF------PPLPSSRYQPRPDATLEHSIS-LPSSA 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1318 RRcGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNP--RLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTAD 1395
Cdd:cd19545   210 SS-GVTLATVLRAAWALVLSRYTGSDDVVFGVTLSGRNAPvpGIEQIVGPTIATVPLRVRIDPEQSVEDFLQTVQKDLLD 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1396 AlahqeLPFEHLVADR------GAGNGPLFRIMYSFSSQEPAGrSAGDVDLEPVPVPVTTCKFD---LVLTVVDGGSTLE 1466
Cdd:cd19545   289 M-----IPFEHTGLQNirrlgpDARAACNFQTLLVVQPALPSS-TSESLELGIEEESEDLEDFSsygLTLECQLSGSGLR 362

                         410       420       430
                  ....*....|....*....|....*....|...
gi 653678460 1467 GVLEYADDLYLPGTAERLVTSLTNLLAAAVRTP 1499
Cdd:cd19545   363 VRARYDSSVISEEQVERLLDQFEHVLQQLASAP 395
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
 1545-2033 1.14e-26

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 117.08  E-value: 1.14e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV-------D 1617
Cdd:cd05959    18 GDKTAFIDDAGSLTYAELEAEARRVAGALRA--LG---VKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVntlltpdD 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPM----ARL----TRIADTVTPAL--------VLTRTGQGGCLPGAEVFGDVpvvaLDRVADRLTAMPDQRPEVtvd 1681
Cdd:cd05959    93 YAYYLedsrARVvvvsGELAPVLAAALtksehtlvVLIVSGGAGPEAGALLLAEL----VAAEAEQLKPAATHADDP--- 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 prglAYSIFTSGSTGQPKGVAVEHigivRYLSWAAASYptAGRRGTLAHSSVGFDLTM--------SALFEPLVSGRGVT 1753
Cdd:cd05959   166 ----AFWLYSSGSTGRPKGVVHLH----ADIYWTAELY--ARNVLGIREDDVCFSAAKlffayglgNSLTFPLSVGATTV 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1754 LM---PADATLADLAEELSGPLAYDYIRLTPSHLRHLVghwTGQELPPAARGWVVGGETLdPALVKQLLELRPDAEVINH 1830
Cdd:cd05959   236 LMperPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAAPN---LPSRDLSSLRLCVSAGEAL-PAEVGERWKARFGLDILDG 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1831 YGPTETV---IGRVVHPVReagelavdsPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEK 1907
Cdd:cd05959   312 IGSTEMLhifLSNRPGRVR---------YGTTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRDKTRDT 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1908 FLpdpaGEpgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ----QLVGW 1982
Cdd:cd05959   383 FQ----GE----WTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVlEAAVVGVEDEdgltKPKAF 454

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 653678460 1983 FIPAEDHPPVTVSA--LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05959   455 VVLRPGYEDSEALEeeLKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKL 507
FUM14_C_NRPS-like cd19545
Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond ...
 64-459 1.29e-26

Condensation domains of nonribosomal peptide synthetases (NRPSs) similar to the ester-bond forming Fusarium verticillioides FUM14 protein; Condensation (C) domains of nonribosomal peptide synthetases (NRPSs) typically catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. However, some C-domains have ester-bond forming activity. This subfamily includes Fusarium verticillioides FUM14 (also known as NRPS8), a bi-domain protein with an ester-bond forming NRPS C-domain, which catalyzes linkages between an aminoacyl/peptidyl-PCP donor and a hydroxyl-containing acceptor. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. FUM14 has an altered active site motif DHTHCD instead of the typical HHxxxD motif seen in other subfamily members. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380467 [Multi-domain]  Cd Length: 395  Bit Score: 114.70  E-value: 1.29e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   64 PGTPL--------------YSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQqtAPAAPVALPVIDV 129
Cdd:cd19545     3 PCTPLqeglmaltarqpgaYVGQRVFELPPDIDLARLQAAWEQVVQANPILRTRIVQSDSGGLLQ--VVVKESPISWTES 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  130 TeaDLPQALRTAAEQPFDLErGPLLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGELPAGVaaPAFRDV 209
Cdd:cd19545    81 T--SLDEYLEEDRAAPMGLG-GPLVRLALVEDPDTERYFVWTIHHALYDGWSLPLILRQVLAAYQGEPVPQP--PPFSRF 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  210 AAWqhgrLAAGELDDQLRYWRQRLAGLpdleipgdrqrpADRDWRAHSVRRELPAATEAAVRRLA----AAHDTTPFTVL 285
Cdd:cd19545   156 VKY----LRQLDDEAAAEFWRSYLAGL------------DPAVFPPLPSSRYQPRPDATLEHSISlpssASSGVTLATVL 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  286 LAAYGTFLHRLTGQDDFAVGVPVAGRG--RTDLESVVG-LFAGmVPVRLDLTGRPSFDEVLSRTvagsrNDFAHPQIPFD 362
Cdd:cd19545   220 RAAWALVLSRYTGSDDVVFGVTLSGRNapVPGIEQIVGpTIAT-VPLRVRIDPEQSVEDFLQTV-----QKDLLDMIPFE 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  363 --------RLVRELRRDRIAGSQLLVQamAGTESTAVALTFGEaagtEQPVDVALAKCD---LDLSVLDDGDRLAVTLVA 431
Cdd:cd19545   294 htglqnirRLGPDARAACNFQTLLVVQ--PALPSSTSESLELG----IEEESEDLEDFSsygLTLECQLSGSGLRVRARY 367

                         410       420
                  ....*....|....*....|....*...
gi 653678460  432 AADLFTPAAAEQLAGQFLSLLAGLTGAP 459
Cdd:cd19545   368 DSSVISEEQVERLLDQFEHVLQQLASAP 395
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
 1532-2033 2.87e-26

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 116.21  E-value: 2.87e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGG 1611
Cdd:PRK08314   11 SLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQ-QECG---VRKGDRVLLYMQNSPQFVIAYYAILRANA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1612 YFIPVDPGYPMARLTRI---------------ADTVTPALVLTRTGQ------GGCLPGAevfGDVPVVALDRVADRLTA 1670
Cdd:PRK08314   87 VVVPVNPMNREEELAHYvtdsgarvaivgselAPKVAPAVGNLRLRHvivaqySDYLPAE---PEIAVPAWLRAEPPLQA 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1671 MPDQR--------------PEVTVDPRGLAYSIFTSGSTGQPKGVAVEHigivRYLSWAAASyptAGRRGTLAHSSVG-- 1734
Cdd:PRK08314  164 LAPGGvvawkealaaglapPPHTAGPDDLAVLPYTSGTTGVPKGCMHTH----RTVMANAVG---SVLWSNSTPESVVla 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1735 ----FDLT--MSALFEPLVSGRGVTLMPA--DATLADLAEelsgplaydyirltpshlRHLVGHWTGqelPP-------- 1798
Cdd:PRK08314  237 vlplFHVTgmVHSMNAPIYAGATVVLMPRwdREAAARLIE------------------RYRVTHWTN---IPtmvvdfla 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1799 ----AARG-----WVVGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVreagelavDSPLP--LGRPLGETR 1867
Cdd:PRK08314  296 spglAERDlsslrYIGGGGAAMPEAVAERLKELTGLDYVEGYGLTETMAQTHSNPP--------DRPKLqcLGIPTFGVD 367

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1868 LQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLP-DpagepGARMYRTGDLVRWRGDGLLDFVGRVDDQV 1945
Cdd:PRK08314  368 ARVIDpETLEELPPGEVGEIVVHGPQVFKGYWNRPEATAEAFIEiD-----GKRFFRTGDLGRMDEEGYFFITDRLKRMI 442

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1946 KLRGYRIELGEIEARMAEHPGVEQA-VVLVRDQ---QLVGWFIPAEDHPPVTVSA--LRRFCAEQLPEFMVPNQWVALDA 2019
Cdd:PRK08314  443 NASGFKVWPAEVENLLYKHPAIQEAcVIATPDPrrgETVKAVVVLRPEARGKTTEeeIIAWAREHMAAYKYPRIVEFVDS 522

                         570
                  ....*....|....
gi 653678460 2020 FPLTPHGKVNHRAL 2033
Cdd:PRK08314  523 LPKSGSGKILWRQL 536
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
 499-979 3.73e-26

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 116.44  E-value: 3.73e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  499 QWCRRTPGAVAVI----EGDT-TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDP 573
Cdd:cd05968    69 KWLADTRTRPALRwegeDGTSrTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFS 148

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  574 DFPVERIAYLLSDARPVLVVTDAATADRLGPDDIT--------------GLVVLEETDTGGYPAT----EPPAVPAGHSA 635
Cdd:cd05968   149 GFGKEAAATRLQDAEAKALITADGFTRRGREVNLKeeadkacaqcptveKVVVVRHLGNDFTPAKgrdlSYDEEKETAGD 228

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 Y-----------VIYTSGSTGRPKGVVIT------RAALDnflaaMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSG 698
Cdd:cd05968   229 GaertesedplmIIYTSGTTGKPKGTVHVhagfplKAAQD-----MYFQFDLKPGDLLTWFTDLGWMMGPWLIFGGLILG 303

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  699 AGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPA----LAGIRVLVG-GEALPAE----LARRLGD 768
Cdd:cd05968   304 ATMVLYDgAPDHPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVnahdLSSLRVLGStGEPWNPEpwnwLFETVGK 383

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  769 AGAEVTNMYGPTETTiWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPiGVLGELHIAGE--GLARGYWNRPGLT 846
Cdd:cd05968   384 GRNPIINYSGGTEIS-GGILGNVLIKPIKPSSFNGPVPGMKADVLDESGKPAR-PEVGELVLLAPwpGMTRGFWRDEDRY 461

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  847 AEKF---LPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPG 922
Cdd:cd05968   462 LETYwsrFDN--------VWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIgVPHPVKG 533

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460  923 DLRLVAYVIPDGPVAPDALRTELSRTLPDYM----IPAVIVPVPDFPTTPNGKLDRAALPA 979
Cdd:cd05968   534 EAIVCFVVLKPGVTPTEALAEELMERVADELgkplSPERILFVKDLPKTRNAKVMRRVIRA 594
PRK07787 PRK07787
acyl-CoA synthetase; Validated
 1536-2036 5.00e-26

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 114.32  E-value: 5.00e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPgwtALRTGDRSLTFAGLDAQANRLAHALHtgalGAPPVGPE-TPvlllldrSPELVVAMLAVLKAGGYFI 1614
Cdd:PRK07787    8 AVAAAADIAD---AVRIGGRVLSRSDLAGAATAVAERVA----GARRVAVLaTP-------TLATVLAVVGALIAGVPVV 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 PVDPGYPMARLTRIADTVTPALVLTRTGQGGclpgaevfGDVPVVALDRVADRLTAMPDqrpevtVDPRGLAYSIFTSGS 1694
Cdd:PRK07787   74 PVPPDSGVAERRHILADSGAQAWLGPAPDDP--------AGLPHVPVRLHARSWHRYPE------PDPDAPALIVYTSGT 139

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEHIGIVRYLSWAAASYPTAGRrGTLAHSSVGFD---LTMSALFEPLVSGRGVTLmpADATLADLAEELSG- 1770
Cdd:PRK07787  140 TGPPKGVVLSRRAIAADLDALAEAWQWTAD-DVLVHGLPLFHvhgLVLGVLGPLRIGNRFVHT--GRPTPEAYAQALSEg 216

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1771 -------PLAYDYIRLTPSHLRHLVGhwtgqelppaARGWVVGGETLdPALVKQLLELRPDAEVINHYGPTETVIgrvVH 1843
Cdd:PRK07787  217 gtlyfgvPTVWSRIAADPEAARALRG----------ARLLVSGSAAL-PVPVFDRLAALTGHRPVERYGMTETLI---TL 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1844 PVREAGElavDSPLPLGRPLGETRLQVLDAWLEAVPVG--AVGELFIGGDGIARGYLGRPALTAEKFLPDPagepgarMY 1921
Cdd:PRK07787  283 STRADGE---RRPGWVGLPLAGVETRLVDEDGGPVPHDgeTVGELQVRGPTLFDGYLNRPDATAAAFTADG-------WF 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1922 RTGDLVRWRGDGLLDFVGRVD-DQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD----QQLVGWFIPAEdhpPVTVS 1995
Cdd:PRK07787  353 RTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVrEAAVVGVPDddlgQRIVAYVVGAD---DVAAD 429

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 653678460 1996 ALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:PRK07787  430 ELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLSE 470
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
 515-933 5.07e-26

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 114.38  E-value: 5.07e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVT 594
Cdd:cd17640     4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 DAatadrlGPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAED 674
Cdd:cd17640    84 EN------DSDDL---------------------------ATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPPQPGD 130

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  675 RVLAtttvsfdiaglelYLPL-----RSGAGVVLAD--ADTARNPAALIDLAGRHRVTLAQATPTLWQALVP-------- 739
Cdd:cd17640   131 RFLS-------------ILPIwhsyeRSAEYFIFACgcSQAYTSIRTLKDDLKRVKPHYIVSVPRLWESLYSgiqkqvsk 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  740 ------ELSGPALAG--IRVLV-GGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEdsIRRGSIGVPIDNTQV 810
Cdd:cd17640   198 sspikqFLFLFFLSGgiFKFGIsGGGALPPHVDTFFEAIGIEVLNGYGLTETSPVVSARRLKC--NVRGSVGRPLPGTEI 275

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  811 YVLDANLH-PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRT-DHQVK 888
Cdd:cd17640   276 KIVDPEGNvVLPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDGW-------FNTGDLGWLTCGGELVLTGRAkDTIVL 348

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*
gi 653678460  889 LRGFRIELGEIETTLINAgPVRRAAAVVREDRPgdlRLVAYVIPD 933
Cdd:cd17640   349 SNGENVEPQPIEEALMRS-PFIEQIMVVGQDQK---RLGALIVPN 389
PRK09088 PRK09088
acyl-CoA synthetase; Validated
 1540-2028 5.43e-26

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 114.52  E-value: 5.43e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1540 QADRTPGWTALR--TGDRSLTFAGLDAQANRLAHALHTGAlgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD 1617
Cdd:PRK09088    4 HARLQPQRLAAVdlALGRRWTYAELDALVGRLAAVLRRRG-----CVDGERLAVLARNSVWLVALHFACARVGAIYVPLN 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPMARLTRIADTVTPALVLtrtgqGGCLPGAevfGDVPVVALDRVADRLTAM-PDQRPevTVDPRGLAYSIFTSGSTG 1696
Cdd:PRK09088   79 WRLSASELDALLQDAEPRLLL-----GDDAVAA---GRTDVEDLAAFIASADALePADTP--SIPPERVSLILFTSGTSG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1697 QPKGVAVEHigivRYLSWAAASYPTAGRRGtlAHSSVGFDLTMSALFEPLVSGRGVTLM----------PADATLADLAE 1766
Cdd:PRK09088  149 QPKGVMLSE----RNLQQTAHNFGVLGRVD--AHSSFLCDAPMFHIIGLITSVRPVLAVggsilvsngfEPKRTLGRLGD 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1767 ELSG-------PLAYDYIRLTP----SHLRHLVGHWTGQELPPAA--RGWVVGGetldpalvkqllelrpdAEVINHYGP 1833
Cdd:PRK09088  223 PALGithyfcvPQMAQAFRAQPgfdaAALRHLTALFTGGAPHAAEdiLGWLDDG-----------------IPMVDGFGM 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1834 TE--TVIGRVVHPVREAGELAVdsplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpd 1911
Cdd:PRK09088  286 SEagTVFGMSVDCDVIRAKAGA-----AGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAF--- 357

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 pagePGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQL--VGW-FIPAE 1987
Cdd:PRK09088  358 ----TGDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIrECAVVGMADAQWgeVGYlAIVPA 433

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 653678460 1988 DHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PRK09088  434 DGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKL 474
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 48-337 6.77e-26

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 112.92  E-value: 6.77e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   48 PLAPGQAGIWFSEQLRPGTPLYSVPLAVRLTGPLDVDALRAALDAVTARHEALRTVFRLgpDG--EPVQ---QTAPaapv 122
Cdd:cd19544     3 PLAPLQEGILFHHLLAEEGDPYLLRSLLAFDSRARLDAFLAALQQVIDRHDILRTAILW--EGlsEPVQvvwRQAE---- 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  123 aLPVIDVT---EADLPQALRTAAE---QPFDLERGPLLRACL-HRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAG 195
Cdd:cd19544    77 -LPVEELTldpGDDALAQLRARFDprrYRLDLRQAPLLRAHVaEDPANGRWLLLLLFHHLISDHTSLELLLEEIQAILAG 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  196 E---LPAgvaAPAFRDVAAwqHGRLAAGELDDQlRYWRQRLA---------GLPDleIPGDrqrpadrDWRAHSVRRELP 263
Cdd:cd19544   156 RaaaLPP---PVPYRNFVA--QARLGASQAEHE-AFFREMLGdvdeptapfGLLD--VQGD-------GSDITEARLALD 220

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  264 AATEAAVRRLAAAHDTTPFTVLLAAYGTFLHRLTGQDDFAVGVPVAGR--GRTDLESVVGLFAGMVPVRLDLTGRP 337
Cdd:cd19544   221 AELAQRLRAQARRLGVSPASLFHLAWALVLARCSGRDDVVFGTVLSGRmqGGAGADRALGMFINTLPLRVRLGGRS 296
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
 481-977 7.35e-26

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 114.86  E-value: 7.35e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  481 WNDTARPLPAA--SLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTL 558
Cdd:PRK06155    9 AARAVDPLPPSerTLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVF 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  559 LAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRL-----GPDDITGLVVLEET---------DTGGYPAT 624
Cdd:PRK06155   89 LGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVEAALLAALeaadpGDLPLPAVWLLDAPasvsvpagwSTAPLPPL 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  625 EPPAVPA----GHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDrVLATTTVSFDIAGLELYLP-LRSGA 699
Cdd:PRK06155  169 DAPAPAAavqpGDTAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADD-VLYTTLPLFHTNALNAFFQaLLAGA 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  700 GVVLADADTArnpAALIDLAGRHrvtlaQATPT-LWQALVPEL----SGPALAGIRVLVG-GEALPAELARRLGDA-GAE 772
Cdd:PRK06155  248 TYVLEPRFSA---SGFWPAVRRH-----GATVTyLLGAMVSILlsqpARESDRAHRVRVAlGPGVPAALHAAFRERfGVD 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  773 VTNMYGPTETTIwSTSGPTSEDsiRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE---GLARGYWNRPGLTAEK 849
Cdd:PRK06155  320 LLDGYGSTETNF-VIAVTHGSQ--RPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADepfAFATGYFGMPEKTVEA 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  850 FlpdpfgppGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVA 928
Cdd:PRK06155  397 W--------RNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFpVPSELGEDEVMAA 468

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 653678460  929 YVIPDGPVA-PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06155  469 VVLRDGTALePVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVL 518
PRK07638 PRK07638
acyl-CoA synthetase; Validated
 505-977 1.05e-25

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 113.72  E-value: 1.05e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETfVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PRK07638   15 PNKIAIKENDRVLTYKDWFESVCKVANWLNEKESKNKT-IAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERL 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDARPVLVVTDAATADRLgpDDITGLVVLEET---DTGGYPATEPPAVPAGHSA-YVIYTSGSTGRPKGVVIT-RAALDN 659
Cdd:PRK07638   94 AISNADMIVTERYKLNDL--PDEEGRVIEIDEwkrMIEKYLPTYAPIENVQNAPfYMGFTSGSTGKPKAFLRAqQSWLHS 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  660 FLAAmAERFPLTAEDRVL-ATTTVSfdiaGLELY---LPLRSGAGVVLADAdtaRNPAALIDLAGRHRVTLAQATPTLWQ 735
Cdd:PRK07638  172 FDCN-VHDFHMKREDSVLiAGTLVH----SLFLYgaiSTLYVGQTVHLMRK---FIPNQVLDKLETENISVMYTVPTMLE 243

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  736 ALVPELSGPAlAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSgpTSEDSIRR-GSIGVPIDNTQVYV 812
Cdd:PRK07638  244 SLYKENRVIE-NKMKIISSGAKWEAEAKEKIKNIfpYAKLYEFYGASELSFVTAL--VDEESERRpNSVGRPFHNVQVRI 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  813 LDANLHPAPIGVLGELHIAGEGLARGYWNrpgltaekflpdpfgppGTRMYRTGDLVRWSAAGDLEYL---------GRT 883
Cdd:PRK07638  321 CNEAGEEVQKGEIGTVYVKSPQFFMGYII-----------------GGVLARELNADGWMTVRDVGYEdeegfiyivGRE 383

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  884 DHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVipDGPVAPDALRTELSRTLPDYMIPAVIVPVPD 963
Cdd:PRK07638  384 KNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII--KGSATKQQLKSFCLQRLSSFKIPKEWHFVDE 461

                         490
                  ....*....|....
gi 653678460  964 FPTTPNGKLDRAAL 977
Cdd:PRK07638  462 IPYTNSGKIARMEA 475
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
 483-974 1.48e-25

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 114.10  E-value: 1.48e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  483 DTARPLPAASLRERFQQWCRRTPG--AVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLA 560
Cdd:PRK12583   10 GGDKPLLTQTIGDAFDATVARFPDreALVVRHQALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  561 VIKAGAAYLPIDPDFPVERIAYLL--SDARPVLVVtDA-------ATADRLGPD---------------DITGLVVLEET 616
Cdd:PRK12583   90 TARIGAILVNINPAYRASELEYALgqSGVRWVICA-DAfktsdyhAMLQELLPGlaegqpgalacerlpELRGVVSLAPA 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  617 DTGGYPA-TEPPAVPAGHSAY----------------VIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLAT 679
Cdd:PRK12583  169 PPPGFLAwHELQARGETVSREalaerqasldrddpinIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVP 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  680 TTVsFDIAGLEL--YLPLRSGAGVVLadADTARNPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRV-LVG 754
Cdd:PRK12583  249 VPL-YHCFGMVLanLGCMTVGACLVY--PNEAFDPLATLQAVEEERCTALYGVPTMFIAELdhPQRGNFDLSSLRTgIMA 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  755 GEALPAELARRLGDA--GAEVTNMYGPTETTIWST-SGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIA 831
Cdd:PRK12583  326 GAPCPIEVMRRVMDEmhMAEVQIAYGMTETSPVSLqTTAADDLERRVETVGRTQPHLEVKVVDPDGATVPRGEIGELCTR 405

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 GEGLARGYWNRPGLTAEKFLPDPFgppgtrMYrTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRR 911
Cdd:PRK12583  406 GYSVMKGYWNNPEATAESIDEDGW------MH-TGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVAD 478

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  912 AAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:PRK12583  479 VQVFGVPDEKYGEEIVAWVRlhPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
 502-972 1.50e-25

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 113.63  E-value: 1.50e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEG--DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVER 579
Cdd:PRK13391    8 QTTPDKPAVIMAstGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAE 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  580 IAYLLSDARPVLVVTDAATAD--------------RL---GPDDITGLVVLEETdTGGYPATEPPAVPAGhsAYVIYTSG 642
Cdd:PRK13391   88 AAYIVDDSGARALITSAAKLDvarallkqcpgvrhRLvldGDGELEGFVGYAEA-VAGLPATPIADESLG--TDMLYSSG 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  643 STGRPKGVV--ITRAALD---NFLAAMAERFPLTAEDRVLATttvsfdiAGLELYLPLRSgAGVVLADADTA-----RNP 712
Cdd:PRK13391  165 TTGRPKGIKrpLPEQPPDtplPLTAFLQRLWGFRSDMVYLSP-------APLYHSAPQRA-VMLVIRLGGTVivmehFDA 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  713 AALIDLAGRHRVTLAQATPTLWQAL--VPELSGPA--LAGIRVLVGGEA-LPAELARRLGD-AGAEVTNMYGPTEttiws 786
Cdd:PRK13391  237 EQYLALIEEYGVTHTQLVPTMFSRMlkLPEEVRDKydLSSLEVAIHAAApCPPQVKEQMIDwWGPIIHEYYAATE----- 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  787 TSGPTSEDS----IRRGSIGVPIDNTqVYVLDANLHPAPIGVLGELHIAGeGLARGYWNRPGLTAEKFLPDPfgppgtrm 862
Cdd:PRK13391  312 GLGFTACDSeewlAHPGTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEG-GRPFEYLNDPAKTAEARHPDG-------- 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  863 yrtgdlvRWSAAGDLEYLG---------RTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVIP 932
Cdd:PRK13391  382 -------TWSTVGDIGYVDedgylyltdRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFgVPNEDLGE-EVKAVVQP 453

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 653678460  933 DGPVAPDA---------LRTELSRtlpdYMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK13391  454 VDGVDPGPalaaeliafCRQRLSR----QKCPRSIDFEDELPRLPTGKL 498
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
 492-973 1.97e-25

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 115.79  E-value: 1.97e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  492 SLRERFQQWCRRTPGAVAVIE-GDTTLSYRELDERANRLARLLmERGAGAETFVAVLLPRS-----ADLLVTLL------ 559
Cdd:PRK08633  616 PLAEAWIDTAKRNWSRLAVADsTGGELSYGKALTGALALARLL-KRELKDEENVGILLPPSvagalANLALLLAgkvpvn 694

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  560 ------------AVIKAG-------------AAYLPIDPDFPVERIAYLLSDARPVLVVTD---AATADRLGPdditgLV 611
Cdd:PRK08633  695 lnytaseaalksAIEQAQiktvitsrkflekLKNKGFDLELPENVKVIYLEDLKAKISKVDkltALLAARLLP-----AR 769

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  612 VLEETDTGGYPATEPpavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTV--SFDIAGl 689
Cdd:PRK08633  770 LLKRLYGPTFKPDDT--------ATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFfhSFGLTV- 840

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  690 ELYLPLRSGAGVVladadTARNP---AALIDLAGRHRVTLAQATPTLWQA------LVPELsgpaLAGIR-VLVGGEALP 759
Cdd:PRK08633  841 TLWLPLLEGIKVV-----YHPDPtdaLGIAKLVAKHRATILLGTPTFLRLylrnkkLHPLM----FASLRlVVAGAEKLK 911

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  760 AELArrlgDA-----GAEVTNMYGPTETT-IWSTSGPTSEDS-------IRRGSIGVPIDNTQVYVLDA-NLHPAPIGVL 825
Cdd:PRK08633  912 PEVA----DAfeekfGIRILEGYGATETSpVASVNLPDVLAAdfkrqtgSKEGSVGMPLPGVAVRIVDPeTFEELPPGED 987

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  826 GELHIAGEGLARGYWNRPGLTAEkFLPDpfgPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIE---TT 902
Cdd:PRK08633  988 GLILIGGPQVMKGYLGDPEKTAE-VIKD---IDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEeelAK 1063

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460  903 LINAGPVRRAAAVVREDRPGDlRLVAyVIPDGPVAPDALRTELSRT-LPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:PRK08633 1064 ALGGEEVVFAVTAVPDEKKGE-KLVV-LHTCGAEDVEELKRAIKESgLPNLWKPSRYFKVEALPLLGSGKLD 1133
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1557-2028 2.21e-25

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 111.84  E-value: 2.21e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPAL 1636
Cdd:cd05973     1 LTFGELRALSARFANALQ--ELG---VGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARL 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1637 VLTRTgqggclpgaevfgdvpvvaldrvadrltampDQRPEVTVDPrglAYSIFTSGSTGQPKGVAVEhigiVRYL-SWA 1715
Cdd:cd05973    76 VVTDA-------------------------------ANRHKLDSDP---FVMMFTSGTTGLPKGVPVP----LRALaAFG 117

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1716 AASYPTAGRRG-----TLAHSSVGFDLtMSALFEPLVSGRGVTLmpadatladLAEELSGPLAYDYIR--------LTPS 1782
Cdd:cd05973   118 AYLRDAVDLRPedsfwNAADPGWAYGL-YYAITGPLALGHPTIL---------LEGGFSVESTWRVIErlgvtnlaGSPT 187

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1783 HLRHLVGHWTGQELPPAARGWVV--GGETLDPALVKqLLELRPDAEVINHYGPTEtvIGRVV-------HPVREAGelav 1853
Cdd:cd05973   188 AYRLLMAAGAEVPARPKGRLRRVssAGEPLTPEVIR-WFDAALGVPIHDHYGQTE--LGMVLanhhaleHPVHAGS---- 260

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1854 dsplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIA----RGYLGRPAltaekflPDPAGepgaRMYRTGDLVRW 1929
Cdd:cd05973   261 -----AGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIANSPlmwfRGYQLPDT-------PAIDG----GYYLTGDTVEF 324

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQ---LVGWFI---PAEDHPPVTVSALRRFCA 2002
Cdd:cd05973   325 DPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVaEAAVIGVPDPErteVVKAFVvlrGGHEGTPALADELQLHVK 404

                         490       500
                  ....*....|....*....|....*.
gi 653678460 2003 EQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd05973   405 KRLSAHAYPRTIHFVDELPKTPSGKI 430
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
 500-977 2.22e-25

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 113.36  E-value: 2.22e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  500 WCRRTPGAVAVIEGDTT-----LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPD 574
Cdd:cd05970    26 MAKEYPDKLALVWCDDAgeeriFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQ 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  575 FPVERIAYLLSDARPVLVVTDA----------ATAD--------RLGPDDITGLVVLEETDTGGYPATEPP---AVPAGH 633
Cdd:cd05970   106 LTAKDIVYRIESADIKMIVAIAednipeeiekAAPEcpskpklvWVGDPVPEGWIDFRKLIKNASPDFERPtanSYPCGE 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  634 SAYVIY-TSGSTGRPKGVVitraalDNFLaamaerFPL----TA-------EDRV---LATTTVSFDIAGlELYLPLRSG 698
Cdd:cd05970   186 DILLVYfSSGTTGMPKMVE------HDFT------YPLghivTAkywqnvrEGGLhltVADTGWGKAVWG-KIYGQWIAG 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  699 AGVVLADADTArNPAALIDLAGRHRVTLAQATPTLWQALVPE-LSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTN 775
Cdd:cd05970   253 AAVFVYDYDKF-DPKALLEKLSKYGVTTFCAPPTIYRFLIREdLSRYDLSSLRyCTTAGEALNPEVFNTFKEKtGIKLME 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  776 MYGPTETTIWSTSGPTSEdsIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE-----GLARGYWNRPGLTAEKF 850
Cdd:cd05970   332 GFGQTETTLTIATFPWME--PKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIRTSkgkpvGLFGGYYKDAEKTAEVW 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  851 LPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAY 929
Cdd:cd05970   410 HDG--------YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTgVPDPIRGQVVKATI 481

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460  930 VIPDGPVAPDALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05970   482 VLAKGYEPSEELKKELqdhvKKVTAPYKYPRIVEFVDELPKTISGKIRRVEI 533
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
 1530-2033 2.85e-25

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 112.42  E-value: 2.85e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKA 1609
Cdd:cd05920    14 DEPLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLR--GLG---IRPGDRVVVQLPNVAEFVVLFFALLRL 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1610 GGYFIPVDPGYPMARLTRIADTVTPALVLtrtgqggclpGAEVFGDVPVVALDRvadrltampdqrpEVTVDPRGLAYSI 1689
Cdd:cd05920    89 GAVPVLALPSHRRSELSAFCAHAEAVAYI----------VPDRHAGFDHRALAR-------------ELAESIPEVALFL 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 FTSGSTGQPKGVAVEHIGivrYLSWAAASYPTAGrrgtLAHSSV---------GFDLTMSALFEPLVSGRGVTLMP---A 1757
Cdd:cd05920   146 LSGGTTGTPKLIPRTHND---YAYNVRASAEVCG----LDQDTVylavlpaahNFPLACPGVLGTLLAGGRVVLAPdpsP 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1758 DATLADLAEELSGPLAydyirLTPSHLRHLVGHWTGQELPPAARGWV-VGGETLDPALVKQLLELRpDAEVINHYGPTET 1836
Cdd:cd05920   219 DAAFPLIEREGVTVTA-----LVPALVSLWLDAAASRRADLSSLRLLqVGGARLSPALARRVPPVL-GCTLQQVFGMAEG 292

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1837 VIG--RVVHPvreaGELAVDSPlplGRPLG-ETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpa 1913
Cdd:cd05920   293 LLNytRLDDP----DEVIIHTQ---GRPMSpDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPD-- 363

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1914 gepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVG----WFIPAEDh 1989
Cdd:cd05920   364 -----GFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLGerscAFVVLRD- 437

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 653678460 1990 PPVTVSALRRFCAEQ-LPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05920   438 PPPSAAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1690-2028 5.20e-25

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 108.90  E-value: 5.20e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 FTSGSTGQPKGVAVEHIGIVRylswaaASYPTAGRRGTLAHSSV--------GFDLTMSALfepLVSGRGVTL-MPAD-- 1758
Cdd:cd05917     9 FTSGTTGSPKGATLTHHNIVN------NGYFIGERLGLTEQDRLcipvplfhCFGSVLGVL---ACLTHGATMvFPSPsf 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1759 ---ATLADLAEE----LSG-PLAY-------DYIRLTPSHLRhlvghwTGqelppaargwVVGGETLDPALVKQLLELRP 1823
Cdd:cd05917    80 dplAVLEAIEKEkctaLHGvPTMFiaelehpDFDKFDLSSLR------TG----------IMAGAPCPPELMKRVIEVMN 143

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1824 DAEVINHYGPTETvigrvvHPVREAGElaVDSPLPL-----GRPLGETRLQVLDAWLEAVP-VGAVGELFIGGDGIARGY 1897
Cdd:cd05917   144 MKDVTIAYGMTET------SPVSTQTR--TDDSIEKrvntvGRIMPHTEAKIVDPEGGIVPpVGVPGELCIRGYSVMKGY 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1898 LGRPALTAEKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQA-VVLVRD 1976
Cdd:cd05917   216 WNDPEKTAEAIDGD-------GWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVqVVGVPD 288

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 653678460 1977 QQL---VGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd05917   289 ERYgeeVCAWIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKI 343
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
 499-974 5.48e-25

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 113.11  E-value: 5.48e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   499 QWCRRTPGAVAVI-EGDT-----TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:TIGR02188   65 RHLEARPDKVAIIwEGDEpgevrKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVF 144

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   573 PDFPVERIAYLLSDARPVLVVT-----------------DAATADrlGPDDITGLVVLEETD-----------------T 618
Cdd:TIGR02188  145 GGFSAEALADRINDAGAKLVITadeglrggkviplkaivDEALEK--CPVSVEHVLVVRRTGnpvvpwvegrdvwwhdlM 222

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   619 GGYPAT-EPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALdNFLAAMAER--FPLTAEDRVLATTTVSFdIAGLE--LYL 693
Cdd:TIGR02188  223 AKASAYcEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGY-LLYAAMTMKyvFDIKDGDIFWCTADVGW-ITGHSyiVYG 300

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   694 PLRSGAGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALVPElsGPA------LAGIRVL--VgGEALPAE--- 761
Cdd:TIGR02188  301 PLANGATTVMFEgVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRL--GDEwvkkhdLSSLRLLgsV-GEPINPEawm 377

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   762 -LARRLGDAGAEVTNMYGPTET---TIWSTSGPTsedSIRRGSIGVPIDNTQVYVLDANLHPAPI-GVLGELHIAGE--G 834
Cdd:TIGR02188  378 wYYKVVGKERCPIVDTWWQTETggiMITPLPGAT---PTKPGSATLPFFGIEPAVVDEEGNPVEGpGEGGYLVIKQPwpG 454

                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   835 LARGYWNRPgltaEKFLPDPFGP-PGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAA 913
Cdd:TIGR02188  455 MLRTIYGDH----ERFVDTYFSPfPG--YYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAA 528

                          490       500       510       520       530       540
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460   914 AVvreDRPGDLR---LVAYVIPDGPVAPDA-----LRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:TIGR02188  529 VV---GIPDDIKgqaIYAFVTLKDGYEPDDelrkeLRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMR 594
PRK07529 PRK07529
AMP-binding domain protein; Validated
 485-977 1.11e-24

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 111.97  E-value: 1.11e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  485 ARPLPAaSLRERFQQWCRRTPGAVAV---IEGD-----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLV 556
Cdd:PRK07529   20 ARDLPA-STYELLSRAAARHPDAPALsflLDADpldrpETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHF 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  557 TLLAVIKAGAAYlPIDPDFPVERIAYLLSDARPVLVVT-----DAATADRLGP--DDITGLVVLEETDTGGY-PATEPPA 628
Cdd:PRK07529   99 ALWGGEAAGIAN-PINPLLEPEQIAELLRAAGAKVLVTlgpfpGTDIWQKVAEvlAALPELRTVVEVDLARYlPGPKRLA 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  629 VPAGH---------------------------------SAYViYTSGSTGRPKgVVITRAALDNFLAAMAERFPLTAEDR 675
Cdd:PRK07529  178 VPLIRrkaharildfdaelarqpgdrlfsgrpigpddvAAYF-HTGGTTGMPK-LAQHTHGNEVANAWLGALLLGLGPGD 255

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATTTVSFDIAGL--ELYLPLRSGAGVVLADADTARNPAALID---LAGRHRVTLAQATPTLWQAL--VPeLSGPALAG 748
Cdd:PRK07529  256 TVFCGLPLFHVNALlvTGLAPLARGAHVVLATPQGYRGPGVIANfwkIVERYRINFLSGVPTVYAALlqVP-VDGHDISS 334

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  749 IR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPtSEDSIRRGSIGVPIDNTQVYVL-----DANLHPAP 821
Cdd:PRK07529  335 LRyALCGAAPLPVEVFRRFEAAtGVRIVEGYGLTEATCVSSVNP-PDGERRIGSVGLRLPYQRVRVVilddaGRYLRDCA 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  822 IGVLGELHIAGEGLARGYwnrpgLTAEK----FLPDpfgppgtRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELG 897
Cdd:PRK07529  414 VDEVGVLCIAGPNVFSGY-----LEAAHnkglWLED-------GWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPA 481

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  898 EIETTLINAGPVRRAAAVVRED-RPGDLRlVAYV--IPDGPVAPDALRTELSRTLPD-YMIPAVIVPVPDFPTTPNGKLD 973
Cdd:PRK07529  482 AIEEALLRHPAVALAAAVGRPDaHAGELP-VAYVqlKPGASATEAELLAFARDHIAErAAVPKHVRILDALPKTAVGKIF 560


                  ....
gi 653678460  974 RAAL 977
Cdd:PRK07529  561 KPAL 564
PRK07470 PRK07470
acyl-CoA synthetase; Validated
 499-972 2.19e-24

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 110.13  E-value: 2.19e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  499 QWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVE 578
Cdd:PRK07470   15 QAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPD 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  579 RIAYLLSDARPVLVVTDAATAD-----RLGPDDITGLVVLEETDTG-GYPA---------TEPPAVPAGHSAYVIYTSGS 643
Cdd:PRK07470   95 EVAYLAEASGARAMICHADFPEhaaavRAASPDLTHVVAIGGARAGlDYEAlvarhlgarVANAAVDHDDPCWFFFTSGT 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  644 TGRPKGVVITRAAL----DNFLAAMaerFP-LTAEDRVLATTTVSFDiAGLELYLPLRSGAGVVLADADTArNPAALIDL 718
Cdd:PRK07470  175 TGRPKAAVLTHGQMafviTNHLADL---MPgTTEQDASLVVAPLSHG-AGIHQLCQVARGAATVLLPSERF-DPAEVWAL 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  719 AGRHRVTLAQATPTLWQALV--PELSGPALAGIRVLV--GGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGP---- 790
Cdd:PRK07470  250 VERHRVTNLFTVPTILKMLVehPAVDRYDHSSLRYVIyaGAPMYRADQKRALAKLGKVLVQYFGLGEVTGNITVLPpalh 329

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  791 TSED--SIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGDL 868
Cdd:PRK07470  330 DAEDgpDARIGTCGFERTGMEVQIQDDEGRELPPGETGEICVIGPAVFAGYYNNPEANAKAFRDGWF--------RTGDL 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  869 VRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAYVIPDG-PVAPDALRTELS 946
Cdd:PRK07470  402 GHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLgVPDPVWGEVGVAVCVARDGaPVDEAELLAWLD 481

                         490       500
                  ....*....|....*....|....*.
gi 653678460  947 RTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:PRK07470  482 GKVARYKLPKRFFFWDALPKSGYGKI 507
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
 509-980 2.31e-24

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 109.79  E-value: 2.31e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  509 AVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDAR 588
Cdd:PRK12406    4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  589 ----------------------PVLVV---TDAATADRLGPDDIT---GLVVLEETDTGGYPATePPAVPAGHSayVIYT 640
Cdd:PRK12406   84 arvliahadllhglasalpagvTVLSVptpPEIAAAYRISPALLTppaGAIDWEGWLAQQEPYD-GPPVPQPQS--MIYT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  641 SGSTGRPKGVviTRAALDNFLAAMAER-----FPLTAEDRVLATTTV------SFDIAGLELylplrsGAGVVLadaDTA 709
Cdd:PRK12406  161 SGTTGHPKGV--RRAAPTPEQAAAAEQmraliYGLKPGIRALLTGPLyhsapnAYGLRAGRL------GGVLVL---QPR 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  710 RNPAALIDLAGRHRVTLAQATPTLWQALvpeLSGPA-------LAGIRVLVGGEA-LPAELARRLGDAGAEVTN-MYGPT 780
Cdd:PRK12406  230 FDPEELLQLIERHRITHMHMVPTMFIRL---LKLPEevrakydVSSLRHVIHAAApCPADVKRAMIEWWGPVIYeYYGST 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  781 ETTIWSTSgpTSEDSIRR-GSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLAR-GYWNRPGLTAEkflpdpfgpp 858
Cdd:PRK12406  307 ESGAVTFA--TSEDALSHpGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRAE---------- 374

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  859 gtrMYRTGdlvrWSAAGDLEYLG---------RTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAY 929
Cdd:PRK12406  375 ---IDRGG----FITSGDVGYLDadgylflcdRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAV 447

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|...
gi 653678460  930 VIPDGPVAPDA--LRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAP 980
Cdd:PRK12406  448 VEPQPGATLDEadIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP 500
PRK13382 PRK13382
bile acid CoA ligase;
502-980 2.72e-24

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 109.85  E-value: 2.72e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PRK13382   54 QRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALA 133

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARPVLVVTD---AATADR------------LGPDDITGLVVLEETDTggyPATEPPAVPAGHSAYVIYTSGSTGR 646
Cdd:PRK13382  134 EVVTREGVDTVIYDeefSATVDRaladcpqatrivAWTDEDHDLTVEVLIAA---HAGQRPEPTGRKGRVILLTSGTTGT 210

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  647 PKGVVITRAALDNFLAAMAERFPLTAEDRVL--ATTTVSFDIAGLELYLPLRSgaGVVladadTAR--NPAALIDLAGRH 722
Cdd:PRK13382  211 PKGARRSGPGGIGTLKAILDRTPWRAEEPTVivAPMFHAWGFSQLVLAASLAC--TIV-----TRRrfDPEATLDLIDRH 283

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLWQ---ALVPE-LSGPALAGIRVLVG-GEALPAELAR----RLGDAgaeVTNMYGPTETTIWSTSGPtse 793
Cdd:PRK13382  284 RATGLAVVPVMFDrimDLPAEvRNRYSGRSLRFAAAsGSRMRPDVVIafmdQFGDV---IYNNYNATEAGMIATATP--- 357

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  794 DSIRRG--SIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYwnRPGLTAEKFlpdpfgppgTRMYRTGDLVRW 871
Cdd:PRK13382  358 ADLRAApdTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY--TSGSTKDFH---------DGFMASGDVGYL 426

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  872 SAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVA--PDALRTELSRTL 949
Cdd:PRK13382  427 DENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGASatPETLKQHVRDNL 506

                         490       500       510
                  ....*....|....*....|....*....|.
gi 653678460  950 PDYMIPAVIVPVPDFPTTPNGKLDRAALPAP 980
Cdd:PRK13382  507 ANYKVPRDIVVLDELPRGATGKILRRELQAR 537
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1558-2033 2.92e-24

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 108.67  E-value: 2.92e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1558 TFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY-PMARLTRIADTVTPAL 1636
Cdd:cd05971     8 TFKELKTASNRFANVLK--EIG---LEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFgPEALEYRLSNSGASAL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1637 VltrtgqggclpgaevfgdvpvvaldrvadrlTAMPDqrpevtvDPrglAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAA 1716
Cdd:cd05971    83 V-------------------------------TDGSD-------DP---ALIIYTSGTTGPPKGALHAHRVLLGHLPGVQ 121

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1717 ASYPTAGRRGTLAHSS-----VG--FDLTMSALFE--PLVSGRgVTLMPADATLaDLAEELSGPLAYdyirLTPSHLRHL 1787
Cdd:cd05971   122 FPFNLFPRDGDLYWTPadwawIGglLDVLLPSLYFgvPVLAHR-MTKFDPKAAL-DLMSRYGVTTAF----LPPTALKMM 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1788 VGHWTGQELPPAA-RGWVVGGETLDPALVKQLLElRPDAEVINHYGPTET--VIGR--VVHPVReagelavdsPLPLGRP 1862
Cdd:cd05971   196 RQQGEQLKHAQVKlRAIATGGESLGEELLGWARE-QFGVEVNEFYGQTECnlVIGNcsALFPIK---------PGSMGKP 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1863 LGETRLQVLDAWLEAVPVGAVGELFIG-GDGIAR-GYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGR 1940
Cdd:cd05971   266 IPGHRVAIVDDNGTPLPPGEVGEIAVElPDPVAFlGYWNNPSATEKKMAGD--------WLLTGDLGRKDSDGYFWYVGR 337

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1941 VDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-----VRDQQLVGWFIPAEDHPP--VTVSALRRFCAEQLPEFMVPNQ 2013
Cdd:cd05971   338 DDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVgipdpIRGEIVKAFVVLNPGETPsdALAREIQELVKTRLAAHEYPRE 417

                         490       500
                  ....*....|....*....|
gi 653678460 2014 WVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05971   418 IEFVNELPRTATGKIRRREL 437
beta-lac_NRPS cd19547
Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis ...
 1084-1407 3.17e-24

Condensation domain of nonribosomal peptide synthetases (NRPSs) similar to Nocardia uniformis NocB which exhibits an unusual cyclization to form beta-lactam rings in pro-nocardicin G synthesis; Nocardia uniformis NRPS NocB acts centrally in the biosynthesis of the nocardicin monocyclic beta-lactam antibiotics. Along with another NRPS NocA, it mediates an unusual cyclization to form beta-lactam rings in the synthesis of the beta-lactam-containing pentapeptide pro-nocardicin G. This small subfamily is related to DCL-type Condensation (C) domains, which catalyze condensation between a D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; domains belonging to this subfamily have an HHHxxxD motif at the active site.


Pssm-ID: 380469 [Multi-domain]  Cd Length: 422  Bit Score: 108.17  E-value: 3.17e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQV--ADPAGGGAAL 1161
Cdd:cd19547     3 PLAPMQEGMLFRGLFWPDSDAYFNQNVLELVGGTDEDVLREAWRRVADRYEILRTGFTWRDRAEPLQYvrDDLAPPWALL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPGTDLAQLLLAEA---ALGFTLATEHPLRAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHR 1238
Cdd:cd19547    83 DWSGEDPDRRAELLERLLAddrAAGLSLADCPLYRLTLVRLGGGRHYLLWSHHHILLDGWCLSLIWGDVFRVYEELAHGR 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1239 EPELdEPALAQADYAVWQRQSAQRGRYAAEldFWRTELTGAVATEVAgdlPRPATPGGGGGAVEFALAPRQIQGIRELAR 1318
Cdd:cd19547   163 EPQL-SPCRPYRDYVRWIRARTAQSEESER--FWREYLRDLTPSPFS---TAPADREGEFDTVVHEFPEQLTRLVNEAAR 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1319 RCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRgNPRL---DNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTAD 1395
Cdd:cd19547   237 GYGVTTNAISQAAWSMLLALQTGARDVVHGLTIAGR-PPELegsEHMVGIFINTIPLRIRLDPDQTVTGLLETIHRDLAT 315

                         330
                  ....*....|..
gi 653678460 1396 ALAHQELPFEHL 1407
Cdd:cd19547   316 TAAHGHVPLAQI 327
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
1536-2033 3.35e-24

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 109.59  E-value: 3.35e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPGWTALR-TGDR-SLTFAGLDAQANRLAHALHTGALGappvgPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:PRK05852   21 LVEVAATRLPEAPALVvTADRiAISYRDLARLVDDLAGQLTRSGLL-----PGDRVALRMGSNAEFVVALLAASRADLVV 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPMARLT-RIADTVTPALVLTRTGQGGCLPGAEVFGDVPV-VALDRVADRLTAMP--DQRPEVTVD---PRGL- 1685
Cdd:PRK05852   96 VPLDPALPIAEQRvRSQAAGARVVLIDADGPHDRAEPTTRWWPLTVnVGGDSGPSGGTLSVhlDAATEPTPAtstPEGLr 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1686 ---AYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSV--GFDLtMSALFEPLVSGrGVTLMPADAT 1760
Cdd:PRK05852  176 pddAMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLyhGHGL-IAALLATLASG-GAVLLPARGR 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1761 LA--DLAEELSGPLAYDYIRLTPSH--LRHLVGHWTGQELPPAARGWVVGGETLDPAlVKQLLELRPDAEVINHYGPTET 1836
Cdd:PRK05852  254 FSahTFWDDIKAVGATWYTAVPTIHqiLLERAATEPSGRKPAALRFIRSCSAPLTAE-TAQALQTEFAAPVVCAFGMTEA 332

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1837 VIGRVVHPVREAG--ELAVDSPLPLGRPLGeTRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpag 1914
Cdd:PRK05852  333 THQVTTTQIEGIGqtENPVVSTGLVGRSTG-AQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANFTDG--- 408

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1915 epgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPAEDHP---- 1990
Cdd:PRK05852  409 -----WLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPresa 483

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 653678460 1991 PVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK05852  484 PPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
 485-704 4.87e-24

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 109.58  E-value: 4.87e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  485 ARPLPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKA 564
Cdd:PRK08279   31 ITPDSKRSLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKL 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  565 GAA-----------------------YLPIDPDF--PVERIAYLLSDARPVLVVTDAATADRLGPDDITGLVvleetdtG 619
Cdd:PRK08279  111 GAVvallntqqrgavlahslnlvdakHLIVGEELveAFEEARADLARPPRLWVAGGDTLDDPEGYEDLAAAA-------A 183

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  620 GYPATEPPA---VPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATttvsfdiaglelyLPLR 696
Cdd:PRK08279  184 GAPTTNPASrsgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYCC-------------LPLY 250


                  ....*...
gi 653678460  697 SGAGVVLA 704
Cdd:PRK08279  251 HNTGGTVA 258
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 517-915 7.42e-24

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 107.27  E-value: 7.42e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPidpdfpveriayllsdarpvlvvtda 596
Cdd:cd05974     1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIP-------------------------- 54

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  597 ATAdRLGPDDITglvvlEETDTGGYPATEPPAVPAGHSAYVIY-TSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDR 675
Cdd:cd05974    55 ATT-LLTPDDLR-----DRVDRGGAVYAAVDENTHADDPMLLYfTSGTTSKPKLVEHTHRSYPVGHLSTMYWIGLKPGDV 128

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 VLATTTVSF-DIAGLELYLPLRSGAGVVLADaDTARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPALAGIRVLVG 754
Cdd:cd05974   129 HWNISSPGWaKHAWSCFFAPWNAGATVFLFN-YARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDLASFDVKLREVVG 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  755 -GEALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSedSIRRGSIGVPIDNTQVYVLDANLHPAPIG----VLGEL 828
Cdd:cd05974   208 aGEPLNPEVIEQVRRAwGLTIRDGYGQTETTALVGNSPGQ--PVKAGSMGRPLPGYRVALLDPDGAPATEGevalDLGDT 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  829 HIAGegLARGYWNRPGLTAEKFlpdpfgppGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGP 908
Cdd:cd05974   286 RPVG--LMKGYAGDPDKTAHAM--------RGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPA 355


                  ....*..
gi 653678460  909 VRRAAAV 915
Cdd:cd05974   356 VAEAAVV 362
PRK06178 PRK06178
acyl-CoA synthetase; Validated
 1522-2028 7.78e-24

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 108.98  E-value: 7.78e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1522 RSEQHVGDDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVV 1601
Cdd:PRK06178   24 REPEYPHGERPLTEYLRAWARERPQRPAIIFYGHVITYAELDELSDRFA-----ALLRQRGVGAGDRVAVFLPNCPQFHI 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1602 AMLAVLKAGGYFIPVDPG------------------------YPMARLTRIADTVTPALVltrTGQGGCLPGAEVFG--- 1654
Cdd:PRK06178   99 VFFGILKLGAVHVPVSPLfrehelsyelndagaevllaldqlAPVVEQVRAETSLRHVIV---TSLADVLPAEPTLPlpd 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1655 --DVPVVALDRVADRLTAMPDQR---PEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVrYLswAAASYPTAGRRGTla 1729
Cdd:PRK06178  176 slRAPRLAAAGAIDLLPALRACTapvPLPPPALDALAALNYTGGTTGMPKGCEHTQRDMV-YT--AAAAYAVAVVGGE-- 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1730 hSSV-----------GFDLTMsaLFePLVSGRGVTLMP---ADATLA--------------DLAEEL---SGPLAYDYir 1778
Cdd:PRK06178  251 -DSVflsflpefwiaGENFGL--LF-PLFSGATLVLLArwdAVAFMAaveryrvtrtvmlvDNAVELmdhPRFAEYDL-- 324

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1779 ltpSHLRHlvghwtgqelpPAARGWVvggETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELAVDS-PL 1857
Cdd:PRK06178  325 ---SSLRQ-----------VRVVSFV---KKLNPDYRQRWRALTGSVLAEAAWGMTETHTCDTFTAGFQDDDFDLLSqPV 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1858 PLGRPLGETRLQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpagEPGarMYRTGDLVRWRGDGLLD 1936
Cdd:PRK06178  388 FVGLPVPGTEFKICDfETGELLPLGAEGEIVVRTPSLLKGYWNKPEATAEAL------RDG--WLHTGDIGKIDEQGFLH 459

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1937 FVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRD-----QQLVGWFIPAEDHpPVTVSALRRFCAEQLPEFMVP 2011
Cdd:PRK06178  460 YLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPdpdkgQVPVAFVQLKPGA-DLTAAALQAWCRENMAVYKVP 538

                         570
                  ....*....|....*..
gi 653678460 2012 NQWVaLDAFPLTPHGKV 2028
Cdd:PRK06178  539 EIRI-VDALPMTATGKV 554
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
 502-979 8.25e-24

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 108.58  E-value: 8.25e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVIEGDTTLSYRE-LDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPdfpVERI 580
Cdd:PRK13388   12 RAGDDTIAVRYGDRTWTWREvLAEAAARAAALIALADPDRPLHVGVLLGNTPEMLFWLAAAALGGYVLVGLNT---TRRG 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  581 AYLLSDARPV---LVVTDAATADRLGPDDITGLVVLEeTDTGGY-------PATEP-PAVPAGHSAYVIYTSGSTGRPKG 649
Cdd:PRK13388   89 AALAADIRRAdcqLLVTDAEHRPLLDGLDLPGVRVLD-VDTPAYaelvaaaGALTPhREVDAMDPFMLIFTSGTTGAPKA 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  650 VVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLP-LRSGAGVVLAD--------ADTARNPAALIDLAG 720
Cdd:PRK13388  168 VRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHSNAVMAGWAPaVASGAAVALPAkfsasgflDDVRRYGATYFNYVG 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RhrvTLAQATPTlwqalvPELSGPALAGIRVLVGGEALP---AELARRLGdagAEVTNMYGPTETTIWSTSGPTSEdsir 797
Cdd:PRK13388  248 K---PLAYILAT------PERPDDADNPLRVAFGNEASPrdiAEFSRRFG---CQVEDGYGSSEGAVIVVREPGTP---- 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  798 RGSIGVPIDNTQVY-----------VLDANLHPA-PIGVLGEL-HIAGEGLARGYWNRPGLTAEKFlpdpfgppgtR--M 862
Cdd:PRK13388  312 PGSIGRGAPGVAIYnpetltecavaRFDAHGALLnADEAIGELvNTAGAGFFEGYYNNPEATAERM----------RhgM 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  863 YRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAA-VVREDRPGDLRLVAYVIPDG-PVAPDA 940
Cdd:PRK13388  382 YWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVyAVPDERVGDQVMAALVLRDGaTFDPDA 461

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|.
gi 653678460  941 LRTELS--RTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPA 979
Cdd:PRK13388  462 FAAFLAaqPDLGTKAWPRYVRIAADLPSTATNKVLKRELIA 502
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
 1548-2033 9.52e-24

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 107.18  E-value: 9.52e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGDRSLTFAGLDAQANRLAHALhTGALGAPPVGPetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTR 1627
Cdd:cd05958     2 TCLRSPEREWTYRDLLALANRIANVL-VGELGIVPGNR---VLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1628 IADTVTPALVLtrtgqggclpgaevfgdvpvvaldrVADRLTAMPDqrpevtvdprgLAYSIFTSGSTGQPKGVAVEHig 1707
Cdd:cd05958    78 ILDKARITVAL-------------------------CAHALTASDD-----------ICILAFTSGTTGAPKATMHFH-- 119

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1708 ivRYLSWAAASYPTAGRRGT-------LAHSSVGFDLTMSALFePLVSGRGVTLMPadATLADLAEELSGPLAYDYIRLT 1780
Cdd:cd05958   120 --RDPLASADRYAVNVLRLReddrfvgSPPLAFTFGLGGVLLF-PFGVGASGVLLE--EATPDLLLSAIARYKPTVLFTA 194

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1781 PSHLRHLVGH--WTGQELPPaARGWVVGGETLDPALVKQLLELRpDAEVINHYGPTEtvigrVVHPVREAGELAVdSPLP 1858
Cdd:cd05958   195 PTAYRAMLAHpdAAGPDLSS-LRKCVSAGEALPAALHRAWKEAT-GIPIIDGIGSTE-----MFHIFISARPGDA-RPGA 266

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGylgrpaltaekflpdpAGEPGARMY------RTGDLVRWRGD 1932
Cdd:cd05958   267 TGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGPTGCRY----------------LADKRQRTYvqggwnITGDTYSRDPD 330

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1933 GLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQ---QLVGWFI---PAEDHPPVTVSALRRFCAEQL 2005
Cdd:cd05958   331 GYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAEcAVVGHPDEsrgVVVKAFVvlrPGVIPGPVLARELQDHAKAHI 410

                         490       500
                  ....*....|....*....|....*...
gi 653678460 2006 PEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05958   411 APYKYPRAIEFVTELPRTATGKLQRFAL 438
PRK06164 PRK06164
acyl-CoA synthetase; Validated
 1536-2023 1.17e-23

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 107.91  E-value: 1.17e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIP 1615
Cdd:PRK06164   15 LLDAHARARPDAVALIDEDRPLSRAELRALVDRLA-----AWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1616 VDPGYPMARLTRIADTVTPALVLTRTGQGGcLPGAEVFGDVP---------VVALDRVAD--------RLTAMPDQRPEV 1678
Cdd:PRK06164   90 VNTRYRSHEVAHILGRGRARWLVVWPGFKG-IDFAAILAAVPpdalpplraIAVVDDAADatpapapgARVQLFALPDPA 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TV--------DPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGR 1750
Cdd:PRK06164  169 PPaaageraaDPDAGALLFTTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLLGALAGGA 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1751 GVTLMP---ADATLADLAEElsgplaydyiRLTpshlrHLVG--------HWTGQELPPAARGWVVGGETLDPALVKQLL 1819
Cdd:PRK06164  249 PLVCEPvfdAARTARALRRH----------RVT-----HTFGndemlrriLDTAGERADFPSARLFGFASFAPALGELAA 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1820 ELRP-DAEVINHYGPTETVIGRVVHPVREAgelAVDSPLPLGRPL-GETRLQVLDAWLEAV-PVGAVGELFIGGDGIARG 1896
Cdd:PRK06164  314 LARArGVPLTGLYGSSEVQALVALQPATDP---VSVRIEGGGRPAsPEARVRARDPQDGALlPDGESGEIEIRAPSLMRG 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1897 YLGRPALTAEKFLPDPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVE--QAVVLV 1974
Cdd:PRK06164  391 YLDNPDATARALTDDG-------YFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAaaQVVGAT 463

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 653678460 1975 RDQQLVGW-FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLT 2023
Cdd:PRK06164  464 RDGKTVPVaFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVT 513
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
 487-977 1.22e-23

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 108.18  E-value: 1.22e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  487 PLPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGA 566
Cdd:PRK07059   19 ASQYPSLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGY 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  567 AYLPIDPDFPVERIAYLLSDA---------------------RPVLVVTDAATADRLGPDD-ITGLVV------------ 612
Cdd:PRK07059   99 VVVNVNPLYTPRELEHQLKDSgaeaivvlenfattvqqvlakTAVKHVVVASMGDLLGFKGhIVNFVVrrvkkmvpawsl 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  613 ---------LEEtdtGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAaldNFLAAMAE-----RFPLTAEDRVLA 678
Cdd:PRK07059  179 pghvrfndaLAE---GARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHR---NIVANVLQmeawlQPAFEKKPRPDQ 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  679 TTTVsfdiAGLELY----------LPLRSGA-GVVLADAdtaRNPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPA 745
Cdd:PRK07059  253 LNFV----CALPLYhifaltvcglLGMRTGGrNILIPNP---RDIPGFIKELKKYQVHIFPAVNTLYNALLnnPDFDKLD 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  746 LAGIRV-LVGGEALPAELARR-LGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRrGSIGVPIDNTQVYVLDANLHPAPIG 823
Cdd:PRK07059  326 FSKLIVaNGGGMAVQRPVAERwLEMTGCPITEGYGLSETSPVATCNPVDATEFS-GTIGLPLPSTEVSIRDDDGNDLPLG 404

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  824 VLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTL 903
Cdd:PRK07059  405 EPGEICIRGPQVMAGYWNRPDETAKVMTADGF-------FRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVV 477

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  904 INAGPVRRAAAV-VREDRPGDLrLVAYVIPDGPVAPDALRTELSRT-LPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07059  478 ASHPGVLEVAAVgVPDEHSGEA-VKLFVVKKDPALTEEDVKAFCKErLTNYKRPKFVEFRTELPKTNVGKILRREL 552
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
 503-979 1.73e-23

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 107.39  E-value: 1.73e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  503 RTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAY 582
Cdd:PRK13383   47 RWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAA 126

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  583 LLSDARPVLVVTDAATADRLGPDDITGLVVLEET----DTGGYPATEPPAvpaghsAYVIYTSGSTGRPKGV---VITRA 655
Cdd:PRK13383  127 ALRAHHISTVVADNEFAERIAGADDAVAVIDPATagaeESGGRPAVAAPG------RIVLLTSGTTGKPKGVpraPQLRS 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  656 ALDNFLAAMaERFPLTAEDRVLATTTVsFDIAGLELYLPLRSGAGVVLA----DADTARNPAALidlagrHRVTLAQATP 731
Cdd:PRK13383  201 AVGVWVTIL-DRTRLRTGSRISVAMPM-FHGLGLGMLMLTIALGGTVLThrhfDAEAALAQASL------HRADAFTAVP 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWqALVPEL-----SGPALAGIRVLV-GGEALPAELARRLGDAGAEVT-NMYGPTETTIWSTSGPtSEDSIRRGSIGVP 804
Cdd:PRK13383  273 VVL-ARILELpprvrARNPLPQLRVVMsSGDRLDPTLGQRFMDTYGDILyNGYGSTEVGIGALATP-ADLRDAPETVGKP 350

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  805 IDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGltaeKFLPDPfgppgtrMYRTGDLVRWSAAGDLEYLGRTD 884
Cdd:PRK13383  351 VAGCPVRILDRNNRPVGPRVTGRIFVGGELAGTRYTDGGG----KAVVDG-------MTSTGDMGYLDNAGRLFIVGRED 419

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  885 HQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDYMIPAVIVPVP 962
Cdd:PRK13383  420 DMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVlhPGSGVDAAQLRDYLKDRVSRFEQPRDINIVS 499

                         490
                  ....*....|....*..
gi 653678460  963 DFPTTPNGKLDRAALPA 979
Cdd:PRK13383  500 SIPRNPTGKVLRKELPG 516
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
 637-974 2.02e-23

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 104.26  E-value: 2.02e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  637 VIYTSGSTGRPKGVVITR----AALDNFlaaMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNp 712
Cdd:cd17635     6 VIFTSGTTGEPKAVLLANktffAVPDIL---QKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGENTTYK- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  713 aALIDLAGRHRVTLAQATPTLWQALVPELSGpALAGIR----VLVGGE-ALPAELARRLGDAGAEVTNMYGPTETTIwST 787
Cdd:cd17635    82 -SLFKILTTNAVTTTCLVPTLLSKLVSELKS-ANATVPslrlIGYGGSrAIAADVRFIEATGLTNTAQVYGLSETGT-AL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  788 SGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmyRTGD 867
Cdd:cd17635   159 CLPTDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLIDGWV--------NTGD 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  868 LVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAA-AVVREDRPGDLRLVAYVIP--DGPVAPDALRTE 944
Cdd:cd17635   231 LGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECAcYEISDEEFGELVGLAVVASaeLDENAIRALKHT 310

                         330       340       350
                  ....*....|....*....|....*....|
gi 653678460  945 LSRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:cd17635   311 IRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
 512-977 3.42e-23

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 106.31  E-value: 3.42e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  512 EGDT-TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPV 590
Cdd:PRK08008   32 GGVVrRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQAS 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  591 LVVTDAA---TADRLGPDD---ITGLVVL---EETDTGGY---------PAT--EPPAVPAGHSAYVIYTSGSTGRPKGV 650
Cdd:PRK08008  112 LLVTSAQfypMYRQIQQEDatpLRHICLTrvaLPADDGVSsftqlkaqqPATlcYAPPLSTDDTAEILFTSGTTSRPKGV 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  651 VITRAaldNFLAA---MAERFPLTAEDRVLaTTTVSFDI-----AGLELylpLRSGAGVVLADADTARnpaALIDLAGRH 722
Cdd:PRK08008  192 VITHY---NLRFAgyySAWQCALRDDDVYL-TVMPAFHIdcqctAAMAA---FSAGATFVLLEKYSAR---AFWGQVCKY 261

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQATPTLwqalvpelsgpalagIRVLVGGEALPAELARRLGDA------------------GAEVTNMYGPTETTI 784
Cdd:PRK08008  262 RATITECIPMM---------------IRTLMVQPPSANDRQHCLREVmfylnlsdqekdafeerfGVRLLTSYGMTETIV 326

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  785 WSTsGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHI---AGEGLARGYWNRPGLTAEKFLPDPFgppgtr 861
Cdd:PRK08008  327 GII-GDRPGDKRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIkgvPGKTIFKEYYLDPKATAKVLEADGW------ 399

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  862 mYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP-DG-PVAPD 939
Cdd:PRK08008  400 -LHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLnEGeTLSEE 478

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 653678460  940 ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK08008  479 EFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 639-974 4.14e-23

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 103.51  E-value: 4.14e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  639 YTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVsFDIAGLEL--YLPLRSGAGVVLADAdtARNPAALI 716
Cdd:cd05917     9 FTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPL-FHCFGSVLgvLACLTHGATMVFPSP--SFDPLAVL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  717 DLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRV-LVGGEALPAELARRL-GDAG-AEVTNMYGPTETTIWSTSGpT 791
Cdd:cd05917    86 EAIEKEKCTALHGVPTMFIAELehPDFDKFDLSSLRTgIMAGAPCPPELMKRViEVMNmKDVTIAYGMTETSPVSTQT-R 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 SEDSI--RRGSIGVPIDNTQVYVLDANLHP-APIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtRMYRTGDL 868
Cdd:cd05917   165 TDDSIekRVNTVGRIMPHTEAKIVDPEGGIvPPVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGD-------GWLHTGDL 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  869 VRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVI--PDGPVAPDALRTEL 945
Cdd:cd05917   238 AVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVgVPDERYGE-EVCAWIRlkEGAELTEEDIKAYC 316

                         330       340
                  ....*....|....*....|....*....
gi 653678460  946 SRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:cd05917   317 KGKIAHYKVPRYVFFVDEFPLTVSGKIQK 345
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
 1557-2033 4.23e-23

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 106.21  E-value: 4.23e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALhtGALGAPPvgPETpVLLLLDRSPELVVAMLAVLKAGgyFIPVDPGYPMA---------RLTR 1627
Cdd:cd05906    40 QSYQDLLEDARRLAAGL--RQLGLRP--GDS-VILQFDDNEDFIPAFWACVLAG--FVPAPLTVPPTydepnarlrKLRH 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1628 IADTVTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLTAMPDQrPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIG 1707
Cdd:cd05906   113 IWQLLGSPVVLTDAELVAEFAGLETLSGLPGIRVLSIEELLDTAADH-DLPQSRPDDLALLMLTSGSTGFPKAVPLTHRN 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1708 IVRYLSWAAASYPTAGRRGTLahSSVGFD----LTMSALFePLVSGRGVTLMPADATLADlaeelsgPLAY-DYI---RL 1779
Cdd:cd05906   192 ILARSAGKIQHNGLTPQDVFL--NWVPLDhvggLVELHLR-AVYLGCQQVHVPTEEILAD-------PLRWlDLIdryRV 261

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1780 T----P----SHLRHLV-----GHWTGQELppaaRGWVVGGETLDPALVKQLLE------LRPDAeVINHYGPTETVIGR 1840
Cdd:cd05906   262 TitwaPnfafALLNDLLeeiedGTWDLSSL----RYLVNAGEAVVAKTIRRLLRllepygLPPDA-IRPAFGMTETCSGV 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1841 VVHPVREAGELAVDSPL-PLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepgaR 1919
Cdd:cd05906   337 IYSRSFPTYDHSQALEFvSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTED-------G 409

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1920 MYRTGDLVrWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ---AVVLVRDQ-----QLVGWFIPAEDHPP 1991
Cdd:cd05906   410 WFRTGDLG-FLDNGNLTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPsftAAFAVRDPgaeteELAIFFVPEYDLQD 488

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460 1992 V---TVSALRRFCAEQL---PEFMVPnqwVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05906   489 AlseTLRAIRSVVSREVgvsPAYLIP---LPKEEIPKTSLGKIQRSKL 533
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
 498-977 6.17e-23

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 104.95  E-value: 6.17e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  498 QQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPV 577
Cdd:PRK09029   10 RHWAQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQ 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  578 ERIAYLLsdarPVLVVTDAATADRLG-PDDITGLVVLEetdtggYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVIT-RA 655
Cdd:PRK09029   90 PLLEELL----PSLTLDFALVLEGENtFSALTSLHLQL------VEGAHAVAWQPQRLATMTLTSGSTGLPKAAVHTaQA 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  656 ALDN---FLAAMaerfPLTAEDRVLatttvsfdiagleLYLPLR--SGAGVV----LADAD-TARNPAALI-DLAGrhrV 724
Cdd:PRK09029  160 HLASaegVLSLM----PFTAQDSWL-------------LSLPLFhvSGQGIVwrwlYAGATlVVRDKQPLEqALAG---C 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  725 TLAQATPT-LWQALvpELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTiwSTSGPTSEDSirRGSIGV 803
Cdd:PRK09029  220 THASLVPTqLWRLL--DNRSEPLSLKAVLLGGAAIPVELTEQAEQQGIRCWCGYGLTEMA--STVCAKRADG--LAGVGS 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  804 PIDNTQVYVLDanlhpapigvlGELHIAGEGLARGYWNRPGLTAekfLPDPFGppgtrMYRTGDLVRWsAAGDLEYLGRT 883
Cdd:PRK09029  294 PLPGREVKLVD-----------GEIWLRGASLALGYWRQGQLVP---LVNDEG-----WFATRDRGEW-QNGELTILGRL 353

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  884 DHQVKLRGFRIELGEIEtTLINAGP-VRRAAAVVRED-----RPgdlrlVAYVIPDGPVAPDALRTELSRTLPDYMIPAV 957
Cdd:PRK09029  354 DNLFFSGGEGIQPEEIE-RVINQHPlVQQVFVVPVADaefgqRP-----VAVVESDSEAAVVNLAEWLQDKLARFQQPVA 427

                         490       500
                  ....*....|....*....|..
gi 653678460  958 IVPVPDfpTTPNG--KLDRAAL 977
Cdd:PRK09029  428 YYLLPP--ELKNGgiKISRQAL 447
PRK05857 PRK05857
fatty acid--CoA ligase;
488-985 9.18e-23

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 105.09  E-value: 9.18e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  488 LPAASLrERFQQWCRRTPGAVAV--IEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAG 565
Cdd:PRK05857   12 LPSTVL-DRVFEQARQQPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLG 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  566 AAYLPIDPDFPVERIAYLLSDARPVLVVTD-----AATADRLGPDDITGLVVLEETDTG--------GYPATEpPAVPAG 632
Cdd:PRK05857   91 AIAVMADGNLPIAAIERFCQITDPAAALVApgskmASSAVPEALHSIPVIAVDIAAVTResehsldaASLAGN-ADQGSE 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  633 HSAYVIYTSGSTGRPKGVVI---TRAALDNFLAAMAERFPltaeDRVLATTTVS----FDIAGLELYLPLRSGAGVVLAD 705
Cdd:PRK05857  170 DPLAMIFTSGTTGEPKAVLLanrTFFAVPDILQKEGLNWV----TWVVGETTYSplpaTHIGGLWWILTCLMHGGLCVTG 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  706 ADtarNPAALIDLAGRHRVTLAQATPTLWQALVPEL--SGPALAGIRVLV-GGEALPAELARRLGDAGAEVTNMYGPTET 782
Cdd:PRK05857  246 GE---NTTSLLEILTTNAVATTCLVPTLLSKLVSELksANATVPSLRLVGyGGSRAIAADVRFIEATGVRTAQVYGLSET 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  783 TIWSTSGPTSEDSIRR---GSIGVPIDNTQVYVLDAN------LHPAPIGVLGELHIAGEGLARGYWNRPGLTAEkFLPD 853
Cdd:PRK05857  323 GCTALCLPTDDGSIVKieaGAVGRPYPGVDVYLAATDgigptaPGAGPSASFGTLWIKSPANMLGYWNNPERTAE-VLID 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  854 PFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAyVIP 932
Cdd:PRK05857  402 GW-------VNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYeIPDEEFGALVGLA-VVA 473

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  933 DGPVAPDALRTELSRTLPDY-------MIPAVIVPVPDFPTTPNGKLDRAALPAPDYGTR 985
Cdd:PRK05857  474 SAELDESAARALKHTIAARFrresepmARPSTIVIVTDIPRTQSGKVMRASLAAAATADK 533
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
 1557-2033 9.52e-23

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 104.12  E-value: 9.52e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPvdpgypmarltrIADTVTPAL 1636
Cdd:cd05969     1 YTFAQLKVLSARFANVLK--SLG---VGKGDRVFVLSPRSPELYFSMLGIGKIGAVICP------------LFSAFGPEA 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1637 VLTRTGQGGclpgAEVfgdvpVVALDRVADRLtampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYlsWAA 1716
Cdd:cd05969    64 IRDRLENSE----AKV-----LITTEELYERT------------DPEDPTLLHYTSGTTGTPKGVLHVHDAMIFY--YFT 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1717 ASYPtagrrgtlahssvgFDL-----------------TMSALFEPLVSGRGVTLMPADATladlAEELSGPLAYDYIRL 1779
Cdd:cd05969   121 GKYV--------------LDLhpddiywctadpgwvtgTVYGIWAPWLNGVTNVVYEGRFD----AESWYGIIERVKVTV 182

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1780 ---TPSHLRHLVGHwtGQELPPA-----ARGWVVGGETLDPALVKQLLELRpDAEVINHYGPTET---VIGRVV-HPVRe 1847
Cdd:cd05969   183 wytAPTAIRMLMKE--GDELARKydlssLRFIHSVGEPLNPEAIRWGMEVF-GVPIHDTWWQTETgsiMIANYPcMPIK- 258

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1848 agelavdsPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGD--GIARGYLGRPALTAEKFlpdPAGepgarMYRTGD 1925
Cdd:cd05969   259 --------PGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGwpSMFRGIWNDEERYKNSF---IDG-----WYLTGD 322

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1926 LVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPA-----EDHPPvtVSALRR- 1999
Cdd:cd05969   323 LAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAfislkEGFEP--SDELKEe 400

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 653678460 2000 ---FCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05969   401 iinFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVL 437
EntF2 COG3319
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ...
 485-1085 2.89e-22

Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442548 [Multi-domain]  Cd Length: 855  Bit Score: 105.17  E-value: 2.89e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  485 ARPLPAASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKA 564
Cdd:COG3319     2 AAAAAAAAAAAAAAAAAAAAAAAAALAAAAAAAAAAALLLLAAALLVALAALALAALALAALLAVALLAAALALAALAAL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  565 GAAYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGST 644
Cdd:COG3319    82 AALALALAAAAAALLLAALALLLALLAALALALLALLLAALLLALAALAAAAAAAALAAAAAAAAALAAAAGLGGGGGGA 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  645 GRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADADTARNPAALIDLAGRHRV 724
Cdd:COG3319   162 GVLVLVLAALLALLLAALLALALALAALLLLALAAALALALLLLLALLLLLLLLLALLLLLLLALLAAAALLALLLALLL 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  725 TLAQATPTLWQALVPELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVP 804
Cdd:COG3319   242 LLLAALLLLLALALLLLLALLLLLGLLALLLALLLLLALLLLAAAAALAAGGTATTAAVTTTAAAAAPGVAGALGPIGGG 321

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  805 IDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTD 884
Cdd:COG3319   322 PGLLVLLVLLVLLLPLLLGVGGGGGGGGGGGGAGGLAGRGLRAAAALRDPAGAGARGRLRRGGDRGRRLGGGLLLGLGRL 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  885 HQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAY--VIPDGPVAPDALRTELSRTLPDYMIPAVIVPVP 962
Cdd:COG3319   402 RLQRLRRGLREELEEAEAALAEAAAVAAAVAAAAAAAAAAAALAAavVAAAALAAAALLLLLLLLLLPPPLPPALLLLLL 481

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  963 DFPTTPNGKLDRAALPAPDYGTRSTARPPRDDRERFLCTAFAEVLGLPEVAVSDNFFELGGHSLLAFRLLARIRDEFGAG 1042
Cdd:COG3319   482 LLLLLLLAALLLAAAAPAAAAAAAAAPAPAAALELALALLLLLLLGLGLVGDDDDFFGGGGGSLLALLLLLLLLALLLRL 561

                         570       580       590       600
                  ....*....|....*....|....*....|....*....|...
gi 653678460 1043 FSLRRLLAAPTVAAVAAELAAETSTGDDAVHLAPVPRDEPLPL 1085
Cdd:COG3319   562 LLLLALLLAPTLAALAAALAAAAAAAALSPLVPLRAGGSGPPL 604
PLN02574 PLN02574
4-coumarate--CoA ligase-like
 505-977 9.82e-22

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 102.23  E-value: 9.82e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDT--TLSYRELDERANRLARLLMER-GAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PLN02574   53 NGDTALIDSSTgfSISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIK 132

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARPVLVVTDAATADRLGPDDITGLVVLEETD-TGGYPATEP--------------PAVPAGHSAYVIYTSGSTGR 646
Cdd:PLN02574  133 KRVVDCSVGLAFTSPENVEKLSPLGVPVIGVPENYDfDSKRIEFPKfyelikedfdfvpkPVIKQDDVAAIMYSSGTTGA 212

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  647 PKGVVITR----AALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLP--LRSGAGVVLADADTARNPAALIDlag 720
Cdd:PLN02574  213 SKGVVLTHrnliAMVELFVRFEASQYEYPGSDNVYLAALPMFHIYGLSLFVVglLSLGSTIVVMRRFDASDMVKVID--- 289

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQATPTLWQALVPELSGPALAGIRVLVGGEALPAELARRLGDAGAEV------TNMYGPTETTIWSTSGPTSED 794
Cdd:PLN02574  290 RFKVTHFPVVPPILMALTKKAKGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTlphvdfIQGYGMTESTAVGTRGFNTEK 369

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  795 SIRRGSIGVPIDNTQVYVLDANLHPA-PIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSA 873
Cdd:PLN02574  370 LSKYSSVGLLAPNMQAKVVDWSTGCLlPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGW-------LRTGDIAYFDE 442

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  874 AGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPD 951
Cdd:PLN02574  443 DGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVrrQGSTLSQEAVINYVAKQVAP 522

                         490       500
                  ....*....|....*....|....*.
gi 653678460  952 YMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PLN02574  523 YKKVRKVVFVQSIPKSPAGKILRREL 548
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 635-977 1.21e-21

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 99.09  E-value: 1.21e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 AYVIYTSGSTGRPKgVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGL--ELYLPLRSGAGVVLADADTARNP 712
Cdd:cd05944     5 AAYFHTGGTTGTPK-LAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSvvTLLTPLASGAHVVLAGPAGYRNP 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  713 AALID---LAGRHRVTLAQATPTLWQALVPELSGPALAGIR-VLVGGEALPAELARRLGDA-GAEVTNMYGPTETTIWST 787
Cdd:cd05944    84 GLFDNfwkLVERYRITSLSTVPTVYAALLQVPVNADISSLRfAMSGAAPLPVELRARFEDAtGLPVVEGYGLTEATCLVA 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  788 SGPtSEDSIRRGSIGVPIDNTQV--YVLDANLH---PAPIGVLGELHIAGEGLARGYWNRPGLTaekflpDPFGPPGtrM 862
Cdd:cd05944   164 VNP-PDGPKRPGSVGLRLPYARVriKVLDGVGRllrDCAPDEVGEICVAGPGVFGGYLYTEGNK------NAFVADG--W 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  863 YRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYV--IPDGPVAPDA 940
Cdd:cd05944   235 LNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVqlKPGAVVEEEE 314

                         330       340       350
                  ....*....|....*....|....*....|....*...
gi 653678460  941 LRTELSRTLPDY-MIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05944   315 LLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
PRK07798 PRK07798
acyl-CoA synthetase; Validated
 1536-2031 1.25e-21

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 101.50  E-value: 1.25e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIP 1615
Cdd:PRK07798    8 LFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLI--AQG---LGPGDHVGIYARNRIEYVEAMLGAFKARAVPVN 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1616 VDPGYPMARLTRIADTVTPALVLTRTGQGGCLpgAEVFGDVP----VVALD------------RVADRLTAMPDQRPEVT 1679
Cdd:PRK07798   83 VNYRYVEDELRYLLDDSDAVALVYEREFAPRV--AEVLPRLPklrtLVVVEdgsgndllpgavDYEDALAAGSPERDFGE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1680 VDPRGLaYSIFTSGSTGQPKGVAVEH--------IGIVRYLSWAAASYPTAGRRGTLAHSSVGFDL-----------TMS 1740
Cdd:PRK07798  161 RSPDDL-YLLYTGGTTGMPKGVMWRQedifrvllGGRDFATGEPIEDEEELAKRAAAGPGMRRFPApplmhgagqwaAFA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1741 AL-------------FEP-----LVSGRGVTLMP--ADATLADLAEELSGPLAYDYirltpSHLRHLVGhwtgqelppaa 1800
Cdd:PRK07798  240 ALfsgqtvvllpdvrFDAdevwrTIEREKVNVITivGDAMARPLLDALEARGPYDL-----SSLFAIAS----------- 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1801 rgwvvGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPvrEAGELAVDSPLPLGRPlgetRLQVLDAWLEAVPV 1880
Cdd:PRK07798  304 -----GGALFSPSVKEALLELLPNVVLTDSIGSSETGFGGSGTV--AKGAVHTGGPRFTIGP----RTVVLDEDGNPVEP 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1881 GAVGELFIGGDG-IARGYLGRPALTAEKFlpdPAGEpGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEA 1959
Cdd:PRK07798  373 GSGEIGWIARRGhIPLGYYKDPEKTAETF---PTID-GVRYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEE 448

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1960 RMAEHPGVEQAVVLVRD-----QQLVGWFIPAEDHPPvTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHR 2031
Cdd:PRK07798  449 ALKAHPDVADALVVGVPderwgQEVVAVVQLREGARP-DLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKADYR 524
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
 502-974 1.60e-21

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 101.87  E-value: 1.60e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  502 RRTPGAVAVI-EGD-----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF 575
Cdd:cd05966    64 KERGDKVAIIwEGDepdqsRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGF 143

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  576 PVERIAYLLSDARPVLVVT-DA-----------ATAD---RLGPDdITGLVVLEETDT----------------GGYPAT 624
Cdd:cd05966   144 SAESLADRINDAQCKLVITaDGgyrggkviplkEIVDealEKCPS-VEKVLVVKRTGGevpmtegrdlwwhdlmAKQSPE 222

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  625 -EPPAVPAGHSAYVIYTSGSTGRPKGVVITRAAldnFL--AAMAER--FPLTAEDRVLATTTVSFdIAGLE--LYLPLRS 697
Cdd:cd05966   223 cEPEWMDSEDPLFILYTSGSTGKPKGVVHTTGG---YLlyAATTFKyvFDYHPDDIYWCTADIGW-ITGHSyiVYGPLAN 298

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  698 GAGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALVPElsGPA------LAGIRVL--VGgEALPAE----LAR 764
Cdd:cd05966   299 GATTVMFEgTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKF--GDEwvkkhdLSSLRVLgsVG-EPINPEawmwYYE 375

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  765 RLGDAGAEVTNMYGPTET-TIWSTSGPtSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGE--GLARGYWN 841
Cdd:cd05966   376 VIGKERCPIVDTWWQTETgGIMITPLP-GATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPwpGMARTIYG 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  842 RPgltaEKFLPDPFGP-PGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVvreDR 920
Cdd:cd05966   455 DH----ERYEDTYFSKfPG--YYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVV---GR 525

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460  921 PGDLR---LVAYVIP-DGPVAPDALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:cd05966   526 PHDIKgeaIYAFVTLkDGEEPSDELRKELrkhvRKEIGPIATPDKIQFVPGLPKTRSGKIMR 587
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
484-977 1.95e-21

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 101.12  E-value: 1.95e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  484 TARPLPAA---SLRERFQQWCRRTPGAVAVIEGD--TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTL 558
Cdd:PRK05852    6 GAAPMASDfgpRIADLVEVAATRLPEAPALVVTAdrIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVAL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  559 LAVIKAGAAYLPIDPDFPV--ERIAYLLSDARPVLVVTDA------------ATADRLGPDDITGLVVLEETDTGGYPAT 624
Cdd:PRK05852   86 LAASRADLVVVPLDPALPIaeQRVRSQAAGARVVLIDADGphdraepttrwwPLTVNVGGDSGPSGGTLSVHLDAATEPT 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  625 EPPAVPAG---HSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGV 701
Cdd:PRK05852  166 PATSTPEGlrpDDAMIMFTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGA 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  702 VLADAD---TARNPAALIDLAGrhrVTLAQATPTLWQALV----PELSGPALAGIRVLVGGEA-LPAELARRLGDA-GAE 772
Cdd:PRK05852  246 VLLPARgrfSAHTFWDDIKAVG---ATWYTAVPTIHQILLeraaTEPSGRKPAALRFIRSCSApLTAETAQALQTEfAAP 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  773 VTNMYGPTETTIWSTSgpTSEDSIRRG-----SIGVPIDNT--QVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGL 845
Cdd:PRK05852  323 VVCAFGMTEATHQVTT--TQIEGIGQTenpvvSTGLVGRSTgaQIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTI 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  846 TAEKFLPDPFgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLR 925
Cdd:PRK05852  401 TAANFTDGWL--------RTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEA 472

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 653678460  926 LVAYVIPDGPVAP--DALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK05852  473 VAAVIVPRESAPPtaEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
PRK06188 PRK06188
acyl-CoA synthetase; Validated
 1534-2036 2.13e-21

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 100.83  E-value: 2.13e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1534 HQLVEAQAdRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGAppvGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:PRK06188   16 HLLVSALK-RYPDRPALVLGDTRLTYGQLADRISRYIQAFE--ALGL---GTGDAVALLSLNRPEVLMAIGAAQLAGLRR 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVdpgYPMARLTR----IAD------TVTPALVLTRTG----QGGCLPGAEVFGDVPV-VALDRVADRLTAMPDQRPEV 1678
Cdd:PRK06188   90 TAL---HPLGSLDDhayvLEDagistlIVDPAPFVERALallaRVPSLKHVLTLGPVPDgVDLLAAAAKFGPAPLVAAAL 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TVDPRGLAYsifTSGSTGQPKGVAVEHIGIVRYLSWAAASY--PTAGRRGT---LAHSSvgfdltmSALFEP-LVSGRGV 1752
Cdd:PRK06188  167 PPDIAGLAY---TGGTTGKPKGVMGTHRSIATMAQIQLAEWewPADPRFLMctpLSHAG-------GAFFLPtLLRGGTV 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1753 TLMP---ADATLADLAEElsgplaydYIRLT---PSHLRHLVGHwtgqelPPAARG------WVV-GGETLDPALVKQLL 1819
Cdd:PRK06188  237 IVLAkfdPAEVLRAIEEQ--------RITATflvPTMIYALLDH------PDLRTRdlssleTVYyGASPMSPVRLAEAI 302

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1820 ElRPDAEVINHYGPTE-----TVIGRVVHPVREAGELAvdsplPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIA 1894
Cdd:PRK06188  303 E-RFGPIFAQYYGQTEapmviTYLRKRDHDPDDPKRLT-----SCGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVM 376

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1895 RGYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVL 1973
Cdd:PRK06188  377 DGYWNRPEETAEAFRDG--------WLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQvAVIG 448

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1974 VRDQQ---LVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:PRK06188  449 VPDEKwgeAVTAVVVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALRAR 514
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
 1529-2031 2.67e-21

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 100.84  E-value: 2.67e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1529 DDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLK 1608
Cdd:PRK05605   30 GDTTLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLR--ALG---VRPGDRVAIVLPNCPQHIVAFYAVLR 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1609 AGGYFIPVDPGYPMARLT---------------RIADTVT--------------------------------PALVLTRT 1641
Cdd:PRK05605  105 LGAVVVEHNPLYTAHELEhpfedhgarvaivwdKVAPTVErlrrttpletivsvnmiaampllqrlalrlpiPALRKARA 184

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1642 GQGGCLPGAevfgdVPVVALdrVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPT 1721
Cdd:PRK05605  185 ALTGPAPGT-----VPWETL--VDAAIGGDGSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLFANAAQGKAWVPG 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1722 AGRRGT-------LAHSsvgFDLTMSALFEPLVSGRGV-------------------TLMPADATL----ADLAEE---- 1767
Cdd:PRK05605  258 LGDGPErvlaalpMFHA---YGLTLCLTLAVSIGGELVllpapdidlildamkkhppTWLPGVPPLyekiAEAAEErgvd 334

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1768 LSGplaydyIRLTPShlrhlvghwtgqelppaargwvvGGETLDPALVkQLLELRPDAEVINHYGPTET---VIGRVVHP 1844
Cdd:PRK05605  335 LSG------VRNAFS-----------------------GAMALPVSTV-ELWEKLTGGLLVEGYGLTETspiIVGNPMSD 384

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1845 VREAGELAVdsplPLgrPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepgarMYRTG 1924
Cdd:PRK05605  385 DRRPGYVGV----PF--PDTEVRIVDPEDPDETMPDGEEGELLVRGPQVFKGYWNRPEETAKSFLDG--------WFRTG 450

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1925 DLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqqlVGwfIPAED-----------HPPVT 1993
Cdd:PRK05605  451 DVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAV-------VG--LPREDgseevvaavvlEPGAA 521

                         570       580       590       600
                  ....*....|....*....|....*....|....*....|
gi 653678460 1994 VS--ALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHR 2031
Cdd:PRK05605  522 LDpeGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRR 561
PRK06145 PRK06145
acyl-CoA synthetase; Validated
 1541-2044 4.93e-21

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 99.58  E-value: 4.93e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDpgy 1620
Cdd:PRK06145   12 ARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDV-----VALLMKNSAAFLELAFAASYLGAVFLPIN--- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 pmARLTRiaDTVtpALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLTAMPDQR---------PEVTVDPRGLAYSIFT 1691
Cdd:PRK06145   84 --YRLAA--DEV--AYILGDAGAKLLLVDEEFDAIVALETPKIVIDAAAQADSRRlaqggleipPQAAVAPTDLVRLMYT 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1692 SGSTGQPKGVAVEHIGivryLSWAAASYP-----TAGRR----GTLAHssVG-FDLTMSALfepLVSGRGVTLM---PAD 1758
Cdd:PRK06145  158 SGTTDRPKGVMHSYGN----LHWKSIDHVialglTASERllvvGPLYH--VGaFDLPGIAV---LWVGGTLRIHrefDPE 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1759 ATLADLAEE-LSG----PLAYDYIRLTPSHLRHLVGHWTgqelppaargWVVGGETLDPAL-VKQLLELRPDAEVINHYG 1832
Cdd:PRK06145  229 AVLAAIERHrLTCawmaPVMLSRVLTVPDRDRFDLDSLA----------WCIGGGEKTPESrIRDFTRVFTRARYIDAYG 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1833 PTETVIGRVVhpvREAGElAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDp 1912
Cdd:PRK06145  299 LTETCSGDTL---MEAGR-EIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGD- 373

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1913 agepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD----QQLVGWFIPAE 1987
Cdd:PRK06145  374 -------WFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVaEAAVIGVHDdrwgERITAVVVLNP 446

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1988 DHpPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALpgptsgRPELE 2044
Cdd:PRK06145  447 GA-TLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVL------RDELN 496
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
 636-974 5.04e-21

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 96.71  E-value: 5.04e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 YVIYTSGSTGRPKgvVITRAAlDNFLAAMAER---FPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLadaDTARNP 712
Cdd:cd17633     4 YIGFTSGTTGLPK--AYYRSE-RSWIESFVCNedlFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIG---QRKFNP 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  713 AALIDLAGRHRVTLAQATPTLWQALVPELSgPALAGIRVLVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWSTSGP 790
Cdd:cd17633    78 KSWIRKINQYNATVIYLVPTMLQALARTLE-PESKIKSIFSSGQKLFESTKKKLKNIfpKANLIEFYGTSELSFITYNFN 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  791 tsEDSIRRGSIGVPIDNTQVYVLDANLhpapiGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpfgppgtrmYRTGDLVR 870
Cdd:cd17633   157 --QESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGYVRGGFSNPDGW------------MSVGDIGY 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  871 WSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTeLSRTLP 950
Cdd:cd17633   218 VDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYSGDKLTYKQLKRF-LKQKLS 296

                         330       340
                  ....*....|....*....|....
gi 653678460  951 DYMIPAVIVPVPDFPTTPNGKLDR 974
Cdd:cd17633   297 RYEIPKKIIFVDSLPYTSSGKIAR 320
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
 1556-2033 6.12e-21

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 98.19  E-value: 6.12e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1556 SLTFAGLDAQANRLAHalHTGALGAPPvgpETPVLLLLDRSPELVVAMLAVLKAGgyfipvdpgypmarltriadtvtpa 1635
Cdd:cd05912     1 SYTFAELFEEVSRLAE--HLAALGVRK---GDRVALLSKNSIEMILLIHALWLLG------------------------- 50

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1636 lvltrtgqggclpgaevfgdVPVVALDRvadRLTA--MPDQRPEVTVDPRGLAYSIFTSGSTGQPKGV------------ 1701
Cdd:cd05912    51 --------------------AEAVLLNT---RLTPneLAFQLKDSDVKLDDIATIMYTSGTTGKPKGVqqtfgnhwwsai 107

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1702 -AVEHIGIVRYLSWAAAsYPtagrrgtLAHSSvgfdlTMSALFEPLVSGRGVTLMP---ADATLADLAEElsgplAYDYI 1777
Cdd:cd05912   108 gSALNLGLTEDDNWLCA-LP-------LFHIS-----GLSILMRSVIYGMTVYLVDkfdAEQVLHLINSG-----KVTII 169

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1778 RLTPSHLRHLVGHwTGQELPPAARGWVVGGETLDPALVKQLLELrpDAEVINHYGPTETViGRVVHPVREAGELAVDSpl 1857
Cdd:cd05912   170 SVVPTMLQRLLEI-LGEGYPNNLRCILLGGGPAPKPLLEQCKEK--GIPVYQSYGMTETC-SQIVTLSPEDALNKIGS-- 243

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1858 pLGRPLGETRLQVLDawlEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpagEPGArmYRTGDLVRWRGDGLLDF 1937
Cdd:cd05912   244 -AGKPLFPVELKIED---DGQPPYEVGEILLKGPNVTKGYLNRPDATEESF------ENGW--FKTGDIGYLDEEGFLYV 311

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1938 VGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqqlVG-----W------FIPAEDhpPVTVSALRRFCAEQLP 2006
Cdd:cd05912   312 LDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGV-------VGipddkWgqvpvaFVVSER--PISEEELIAYCSEKLA 382

                         490       500
                  ....*....|....*....|....*..
gi 653678460 2007 EFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05912   383 KYKVPKKIYFVDELPRTASGKLLRHEL 409
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
 493-977 8.29e-21

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 99.34  E-value: 8.29e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  493 LRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:PRK06710   26 LHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTN 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFPVERIAYLLSD--ARPVLV-------VTDAATADRLG-------------PDDI---------TGLVV-LEETDTGG 620
Cdd:PRK06710  106 PLYTERELEYQLHDsgAKVILCldlvfprVTNVQSATKIEhvivtriadflpfPKNLlypfvqkkqSNLVVkVSESETIH 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  621 Y---------PATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAAL-DNFLAAMAERFPLTAEDRVLATTTVSFDIAGLE 690
Cdd:PRK06710  186 LwnsvekevnTGVEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLvSNTLMGVQWLYNCKEGEEVVLGVLPFFHVYGMT 265

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  691 --LYLPLRSGAGVVLADADTARnpaALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRVLVGGEA-LPAELARR 765
Cdd:PRK06710  266 avMNLSIMQGYKMVLIPKFDMK---MVFEAIKKHKVTLFPGAPTIYIALLnsPLLKEYDISSIRACISGSApLPVEVQEK 342

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  766 LGD-AGAEVTNMYGPTETTIWSTSGPTSEDSIRrGSIGVPIDNTQVYVLDANLHPA-PIGVLGELHIAGEGLARGYWNRP 843
Cdd:PRK06710  343 FETvTGGKLVEGYGLTESSPVTHSNFLWEKRVP-GSIGVPWPDTEAMIMSLETGEAlPPGEIGEIVVKGPQIMKGYWNKP 421

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  844 GLTAeKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPG 922
Cdd:PRK06710  422 EETA-AVLQDGW-------LHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIgVPDPYRG 493

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  923 DLRLVAYVIPDGPVAPDALRTELSRT-LPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06710  494 ETVKAFVVLKEGTECSEEELNQFARKyLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
 637-882 1.04e-20

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 95.80  E-value: 1.04e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  637 VIYTSGSTGRPKGVVITRAaldNFLAA---MAERFPLTAEDRVLATTTVsFDIAGLELYLPL--RSGAGVVLADADtarn 711
Cdd:cd17637     5 IIHTAAVAGRPRGAVLSHG---NLIAAnlqLIHAMGLTEADVYLNMLPL-FHIAGLNLALATfhAGGANVVMEKFD---- 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  712 PAALIDLAGRHRVTLAQATPTLWQALVPEL--SGPALAGIRVLVGGEAlPAELARRLGDAGAEVTNMYGPTETTIWSTSG 789
Cdd:cd17637    77 PAEALELIEEEKVTLMGSFPPILSNLLDAAekSGVDLSSLRHVLGLDA-PETIQRFEETTGATFWSLYGQTETSGLVTLS 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSEdsiRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLV 869
Cdd:cd17637   156 PYRE---RPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTFRNG--------WHHTGDLG 224

                         250
                  ....*....|...
gi 653678460  870 RWSAAGDLEYLGR 882
Cdd:cd17637   225 RFDEDGYLWYAGR 237
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
635-977 1.40e-20

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 95.88  E-value: 1.40e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERfpLTAEDRVLATTTvSFDIAGLELYL-PLRSGAGVVLADADTARNPA 713
Cdd:PRK07824   38 ALVVATSGTTGTPKGAMLTAAALTASADATHDR--LGGPGQWLLALP-AHHIAGLQVLVrSVIAGSEPVELDVSAGFDPT 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  714 ALIDLAGRHRV--TLAQATPT-LWQALVPELSGPALAGI-RVLVGGEALPAELARRLGDAGAEVTNMYGPTEttiwsTSG 789
Cdd:PRK07824  115 ALPRAVAELGGgrRYTSLVPMqLAKALDDPAATAALAELdAVLVGGGPAPAPVLDAAAAAGINVVRTYGMSE-----TSG 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSEDsirrgsiGVPIDNTQVYVLDanlhpapigvlGELHIAGEGLARGYWNRPGltaekflPDPFGPPGtrMYRTGDLV 869
Cdd:PRK07824  190 GCVYD-------GVPLDGVRVRVED-----------GRIALGGPTLAKGYRNPVD-------PDPFAEPG--WFRTDDLG 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  870 RWSaAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVIPDGPVAP--DALRTELS 946
Cdd:PRK07824  243 ALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFgLPDDRLGQ-RVVAAVVGDGGPAPtlEALRAHVA 320

                         330       340       350
                  ....*....|....*....|....*....|.
gi 653678460  947 RTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07824  321 RTLDRTAAPRELHVVDELPRRGIGKVDRRAL 351
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
491-977 1.76e-20

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 98.20  E-value: 1.76e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  491 ASLRERFQQWCRRTPGAVAVIEGDTTLSYRELDERANRLARLL-----MERGagaeTFVAVLLPrsaDLL---VTLLAVI 562
Cdd:PRK08974   23 QSLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLqnglgLKKG----DRVALMMP---NLLqypIALFGIL 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  563 KAGAAYLPIDPDFPVERIAYLLSD--ARPVLVVTD-AATAD--------------RLG---------------------- 603
Cdd:PRK08974   96 RAGMIVVNVNPLYTPRELEHQLNDsgAKAIVIVSNfAHTLEkvvfktpvkhviltRMGdqlstakgtlvnfvvkyikrlv 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  604 -----PDDITGLVVLEEtdtGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVIT-RAALDNFLAAMAERFPLTAEDRVL 677
Cdd:PRK08974  176 pkyhlPDAISFRSALHK---GRRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLThRNMLANLEQAKAAYGPLLHPGKEL 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  678 ATTtvsfdiaGLELY----------LPLRSGAGVVLADadTARNPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPA 745
Cdd:PRK08974  253 VVT-------ALPLYhifaltvnclLFIELGGQNLLIT--NPRDIPGFVKELKKYPFTAITGVNTLFNALLnnEEFQELD 323

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  746 LAGIRVLVGG-EALPAELARRLGDA-GAEVTNMYGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTQVYVLDANLHPAPIG 823
Cdd:PRK08974  324 FSSLKLSVGGgMAVQQAVAERWVKLtGQYLLEGYGLTECSPLVSVNPYDLDY-YSGSIGLPVPSTEIKLVDDDGNEVPPG 402

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  824 VLGELHIAGEGLARGYWNRPGLTAEkFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTL 903
Cdd:PRK08974  403 EPGELWVKGPQVMLGYWQRPEATDE-VIKDGW-------LATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVV 474

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  904 INAGPVRRAAAV-VREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK08974  475 MLHPKVLEVAAVgVPSEVSGEAVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
1522-1973 1.93e-20

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 98.25  E-value: 1.93e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1522 RSEQHVGDDRTVHQLVEAQADRTPGWTALRTGD----RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSP 1597
Cdd:COG1022     2 SEFSDVPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLL--ALG---VKPGDRVAILSDNRP 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1598 ELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQ--------GGCLP---------GAEVFGDVPVVA 1660
Cdd:COG1022    77 EWVIADLAILAAGAVTVPIYPTSSAEEVAYILNDSGAKVLFVEDQEqldkllevRDELPslrhivvldPRGLRDDPRLLS 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1661 LDRVADRLTAMPDQ----RPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHigivRYLSWAAASYPTAGRRGT--------- 1727
Cdd:COG1022   157 LDELLALGREVADPaeleARRAAVKPDDLATIIYTSGTTGRPKGVMLTH----RNLLSNARALLERLPLGPgdrtlsflp 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1728 LAHSsvgFDLTMSALFepLVSG------RGVTLMPAD--------------------ATLADLAEELSGP---------- 1771
Cdd:COG1022   233 LAHV---FERTVSYYA--LAAGatvafaESPDTLAEDlrevkptfmlavprvwekvyAGIQAKAEEAGGLkrklfrwala 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1772 LAYDYIRL-----TPS---HLRHLVGHW---------TGQELppaaRGWVVGGETLDPALVK-------QLLELrpdaev 1827
Cdd:COG1022   308 VGRRYARArlagkSPSlllRLKHALADKlvfsklreaLGGRL----RFAVSGGAALGPELARffralgiPVLEG------ 377

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1828 inhYGPTET--VIGrvvhpVREAGELAVDSplpLGRPLGETRLQVldawleavpvGAVGELFIGGDGIARGYLGRPALTA 1905
Cdd:COG1022   378 ---YGLTETspVIT-----VNRPGDNRIGT---VGPPLPGVEVKI----------AEDGEILVRGPNVMKGYYKNPEATA 436

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 1906 EKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLR-GYRIELGEIEARMAEHPGVEQAVVL 1973
Cdd:COG1022   437 EAFDAD-------GWLHTGDIGELDEDGFLRITGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVV 498
PRK08316 PRK08316
acyl-CoA synthetase; Validated
 1541-2052 2.33e-20

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 97.70  E-value: 2.33e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV---- 1616
Cdd:PRK08316   21 ARRYPDKTALVFGDRSWTYAELDAAVNRVAAALL--DLG---LKKGDRVAALGHNSDAYALLWLACARAGAVHVPVnfml 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1617 ---DPGY----PMARL----TRIADTVTPALVLTRTGQGGCLPGAEvfGDVPVVALDRVADRLTAMPDQRPEVTVDPRGL 1685
Cdd:PRK08316   96 tgeELAYildhSGARAflvdPALAPTAEAALALLPVDTLILSLVLG--GREAPGGWLDFADWAEAGSVAEPDVELADDDL 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1686 AYSIFTSGSTGQPKGVAVEHIGIV-RYLSWAAASYPTAGrrgtlahssvgfDLTMSALfePL--VSGRGVTLMPADATLA 1762
Cdd:PRK08316  174 AQILYTSGTESLPKGAMLTHRALIaEYVSCIVAGDMSAD------------DIPLHAL--PLyhCAQLDVFLGPYLYVGA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1763 D--LAEELSGPLAYDYI---RLT-----P----SHLRHLVghWTGQELPPAARGWVvgGETLDPALVkqLLELR---PDA 1825
Cdd:PRK08316  240 TnvILDAPDPELILRTIeaeRITsffapPtvwiSLLRHPD--FDTRDLSSLRKGYY--GASIMPVEV--LKELRerlPGL 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1826 EVINHYGPTE-----TVIGRVVHPVReagelavdsPLPLGRPL--GETRlqVLDAWLEAVPVGAVGELFIGGDGIARGYL 1898
Cdd:PRK08316  314 RFYNCYGQTEiaplaTVLGPEEHLRR---------PGSAGRPVlnVETR--VVDDDGNDVAPGEVGEIVHRSPQLMLGYW 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1899 GRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ 1977
Cdd:PRK08316  383 DDPEKTAEAFRGG--------WFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVaEVAVIGLPDP 454

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1978 ---QLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRalpgptsgrpELEDRYQAPRT 2052
Cdd:PRK08316  455 kwiEAVTAVVVPKAGATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKR----------ELRERYAGAFT 522
PRK07867 PRK07867
acyl-CoA synthetase; Validated
 509-979 2.54e-20

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 97.44  E-value: 2.54e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  509 AVIEGDTTLSYRELDERANRLARLLMER-GAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPdfpVERIAYLLSDA 587
Cdd:PRK07867   21 GLYFEDSFTSWREHIRGSAARAAALRARlDPTRPPHVGVLLDNTPEFSLLLGAAALSGIVPVGLNP---TRRGAALARDI 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  588 RPV---LVVTDAATADRLgpDDITGLVVLEETDT-------GGYPATEPPAVPAGHS--AYVIYTSGSTGRPKGVVITRA 655
Cdd:PRK07867   98 AHAdcqLVLTESAHAELL--DGLDPGVRVINVDSpawadelAAHRDAEPPFRVADPDdlFMLIFTSGTSGDPKAVRCTHR 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  656 ALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYLP-LRSGAGVVLADADTArnpAALIDLAGRHRVTLAQ--ATPT 732
Cdd:PRK07867  176 KVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVaLAAGASIALRRKFSA---SGFLPDVRRYGATYANyvGKPL 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  733 LWQALVPELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEDsirrGSIGVPIDNTQVY- 811
Cdd:PRK07867  253 SYVLATPERPDDADNPLRIVYGNEGAPGDIARFARRFGCVVVDGFGSTEGGVAITRTPDTPP----GALGPLPPGVAIVd 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  812 ----------VLDANLHPAPIGVLGEL-HIAGEGLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYL 880
Cdd:PRK07867  329 pdtgtecppaEDADGRLLNADEAIGELvNTAGPGGFEGYYNDPEADAERMRGG--------VYWSGDLAYRDADGYAYFA 400

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  881 GRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRP-GDLRLVAYVIPDGPV-APDALRTELS--RTLPDYMIPA 956
Cdd:PRK07867  401 GRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVvGDQVMAALVLAPGAKfDPDAFAEFLAaqPDLGPKQWPS 480

                         490       500
                  ....*....|....*....|...
gi 653678460  957 VIVPVPDFPTTPNGKLDRAALPA 979
Cdd:PRK07867  481 YVRVCAELPRTATFKVLKRQLSA 503
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
 1530-2028 2.58e-20

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 97.43  E-value: 2.58e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTAL------RTGDRSLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAM 1603
Cdd:PRK13295   23 DRTINDDLDACVASCPDKTAVtavrlgTGAPRRFTYRELAALVDRVAVGL--ARLG---VGRGDVVSCQLPNWWEFTVLY 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1604 LAVLKAGGYFIPVDP--------------------------GYPMARLTRIADTVTPAL--VLTRTGQGgclpgAEVFGD 1655
Cdd:PRK13295   98 LACSRIGAVLNPLMPifrerelsfmlkhaeskvlvvpktfrGFDHAAMARRLRPELPALrhVVVVGGDG-----ADSFEA 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1656 V---PVVALDRVADRLTAMPdqRPevtvDPRGLAYSIFTSGSTGQPKGVAVEHIGIVrylswaAASYPTAGRRG------ 1726
Cdd:PRK13295  173 LlitPAWEQEPDAPAILARL--RP----GPDDVTQLIYTSGTTGEPKGVMHTANTLM------ANIVPYAERLGlgaddv 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1727 -----TLAHSSvGFdltMSALFEPLVSGRGVTLM----PADAtlADLAEE-------LSGPLAYDYIRlTPSHLRHLVgh 1790
Cdd:PRK13295  241 ilmasPMAHQT-GF---MYGLMMPVMLGATAVLQdiwdPARA--AELIRTegvtftmASTPFLTDLTR-AVKESGRPV-- 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1791 wtgqelpPAARGWVVGGETLDPALVKQLLElRPDAEVINHYGPTETVIGRVVHPvREAGELAVDSPlplGRPLGETRLQV 1870
Cdd:PRK13295  312 -------SSLRTFLCAGAPIPGALVERARA-ALGAKIVSAWGMTENGAVTLTKL-DDPDERASTTD---GCPLPGVEVRV 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1871 LDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEkflpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGY 1950
Cdd:PRK13295  380 VDADGAPLPAGQIGRLQVRGCSNFGGYLKRPQLNGT----DADG-----WFDTGDLARIDADGYIRISGRSKDVIIRGGE 450

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1951 RIELGEIEARMAEHPGVEQ-AVVLVRDQQL---VGWFIPAEDHPPVTVSALRRFCAEQ-LPEFMVPNQWVALDAFPLTPH 2025
Cdd:PRK13295  451 NIPVVEIEALLYRHPAIAQvAIVAYPDERLgerACAFVVPRPGQSLDFEEMVEFLKAQkVAKQYIPERLVVRDALPRTPS 530


                  ...
gi 653678460 2026 GKV 2028
Cdd:PRK13295  531 GKI 533
PRK07638 PRK07638
acyl-CoA synthetase; Validated
 1541-2048 3.14e-20

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 96.77  E-value: 3.14e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgalgAPPVGPETpVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY 1620
Cdd:PRK07638   11 ASLQPNKIAIKENDRVLTYKDWFESVCKVANWLN-----EKESKNKT-IAILLENRIEFLQLFAGAAMAGWTCVPLDIKW 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 PMA-RLTRIADTvTPALVLTRTGQGGCLPGAEVfgdvPVVALDRVaDRLTAMPDQRPEVTVDPRGLA-YSIFTSGSTGQP 1698
Cdd:PRK07638   85 KQDeLKERLAIS-NADMIVTERYKLNDLPDEEG----RVIEIDEW-KRMIEKYLPTYAPIENVQNAPfYMGFTSGSTGKP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1699 KGVAVEHigivryLSWAAASYPTAGR-----------RGTLAHSSVGFDlTMSALFeplvSGRGVTLMPaDATLADLAEE 1767
Cdd:PRK07638  159 KAFLRAQ------QSWLHSFDCNVHDfhmkredsvliAGTLVHSLFLYG-AISTLY----VGQTVHLMR-KFIPNQVLDK 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1768 LSGPlAYDYIRLTPSHLRHLVghwTGQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTEtvIGRVVHPVRE 1847
Cdd:PRK07638  227 LETE-NISVMYTVPTMLESLY---KENRVIENKMKIISSGAKWEAEAKEKIKNIFPYAKLYEFYGASE--LSFVTALVDE 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1848 AGELAVDSplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEkflPDPAGepgarmYRTGDLV 1927
Cdd:PRK07638  301 ESERRPNS---VGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYIIGGVLARE---LNADG------WMTVRDV 368

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1928 RWRG-DGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPAEDHPPVTVSALRRFCAEQLP 2006
Cdd:PRK07638  369 GYEDeEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAIIKGSATKQQLKSFCLQRLS 448

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 653678460 2007 EFMVPNQWVALDAFPLTPHGKVNHRALpgpTSGRPELEDRYQ 2048
Cdd:PRK07638  449 SFKIPKEWHFVDEIPYTNSGKIARMEA---KSWIENQEKIYE 487
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
 1556-2033 3.60e-20

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 96.01  E-value: 3.60e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1556 SLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVtpa 1635
Cdd:cd05935     1 SLTYLELLEVVKKLA-----SFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDS--- 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1636 lvltrtgqggclpgaevfGDVPVVALDRVADrltampdqrpevtvdprgLAYSIFTSGSTGQPKGVAVEHIGIvrylsWA 1715
Cdd:cd05935    73 ------------------GAKVAVVGSELDD------------------LALIPYTSGTTGLPKGCMHTHFSA-----AA 111

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1716 AASYPTAGRRGTLAHSSVG----FDLT-MSALFEPLVSGRGVTLMPA---DATLADLAEelsgplaydyirltpshlRHL 1787
Cdd:cd05935   112 NALQSAVWTGLTPSDVILAclplFHVTgFVGSLNTAVYVGGTYVLMArwdRETALELIE------------------KYK 173

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1788 VGHWTGqeLPPA-----------ARGW----VV--GGETLDPALVKQLLELrPDAEVINHYGPTETVIGRVVHPvreAGE 1850
Cdd:cd05935   174 VTFWTN--IPTMlvdllatpefkTRDLsslkVLtgGGAPMPPAVAEKLLKL-TGLRFVEGYGLTETMSQTHTNP---PLR 247

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1851 LAVDSplpLGRPLGETRLQVLDAW-LEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPagepGARMYRTGDLVRW 1929
Cdd:cd05935   248 PKLQC---LGIP*FGVDARVIDIEtGRELPPNEVGEIVVRGPQIFKGYWNRPEETEESFIEIK----GRRFFRTGDLGYM 320

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPA------EDHPPVTVSALRRFCAE 2003
Cdd:cd05935   321 DEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAfivlrpEYRGKVTEEDIIEWARE 400

                         490       500       510
                  ....*....|....*....|....*....|
gi 653678460 2004 QLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05935   401 QMAAYKYPREVEFVDELPRSASGKILWRLL 430
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
 485-878 3.96e-20

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 97.26  E-value: 3.96e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  485 ARPLPAASLR--ERFQQWCRRTPGAVAVIEGDT-----TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVT 557
Cdd:PRK08180   31 AEPLGDYPRRltDRLVHWAQEAPDRVFLAERGAdggwrRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEHALL 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  558 LLAVIKAGAAYLPIDP-------DFpvERIAYLLSDARPVLVVTDAATA-----DRLGPDDITglVVLEETDTGGYPAT- 624
Cdd:PRK08180  111 ALAAMYAGVPYAPVSPayslvsqDF--GKLRHVLELLTPGLVFADDGAAfaralAAVVPADVE--VVAVRGAVPGRAATp 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  625 --------EPPAVPAGHS-------AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFP-LTAEDRVLA-------TTT 681
Cdd:PRK08180  187 faallatpPTAAVDAAHAavgpdtiAKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPfLAEEPPVLVdwlpwnhTFG 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  682 VSFDIaGLELY----LPLRSGAGVVLADADTARNpaaLIDLAGrhrvTLAQATPTLWQALVPELSG-PALA-----GIRV 751
Cdd:PRK08180  267 GNHNL-GIVLYnggtLYIDDGKPTPGGFDETLRN---LREISP----TVYFNVPKGWEMLVPALERdAALRrrffsRLKL 338

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  752 LV-GGEALPAELARRLgDAGAE--------VTNMYGPTET--TIWSTSGPTSedsiRRGSIGVPIDNTQVYVldanlhpA 820
Cdd:PRK08180  339 LFyAGAALSQDVWDRL-DRVAEatcgerirMMTGLGMTETapSATFTTGPLS----RAGNIGLPAPGCEVKL-------V 406

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460  821 PIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLE 878
Cdd:PRK08180  407 PVGGKLEVRVKGPNVTPGYWRAPELTAEAFDEEGY-------YRSGDAVRFVDPADPE 457
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
 516-933 5.08e-20

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 96.52  E-value: 5.08e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGaaylpidpdFPV---------ERIAYLLSD 586
Cdd:cd17639     5 YMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQN---------IPIvtvyatlgeDALIHSLNE 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  587 ARPVLVVTDAatadrlGPDDItglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAE 666
Cdd:cd17639    76 TECSAIFTDG------KPDDL---------------------------ACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGD 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  667 RFP--LTAEDRVLAtttvsfdiaglelYLPL-----------------RSGAGVV--LADADTAR--------NPAALI- 716
Cdd:cd17639   123 RVPelLGPDDRYLA-------------YLPLahifelaaenvclyrggTIGYGSPrtLTDKSKRGckgdltefKPTLMVg 189

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  717 ---------------------------DLAGRHRVTLAQATP--TLWQALVPELSGPALAG-IR-VLVGGEALPAELARR 765
Cdd:cd17639   190 vpaiwdtirkgvlaklnpmgglkrtlfWTAYQSKLKALKEGPgtPLLDELVFKKVRAALGGrLRyMLSGGAPLSADTQEF 269

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  766 LGDAGAEVTNMYGPTETtiwSTSGPTSE-DSIRRGSIGVPIDNTQV---------YVLDAnlhPAPigvLGELHIAGEGL 835
Cdd:cd17639   270 LNIVLCPVIQGYGLTET---CAGGTVQDpGDLETGRVGPPLPCCEIklvdweeggYSTDK---PPP---RGEILIRGPNV 340

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  836 ARGYWNRPGLTAEKFLPDpfgppgtRMYRTGDLVRWSAAGDLEYLGRTDHQVKLR-GFRIELGEIETTLINAGPVRRAAA 914
Cdd:cd17639   341 FKGYYKNPEKTKEAFDGD-------GWFHTGDIGEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYRSNPLVNNICV 413

                         490
                  ....*....|....*....
gi 653678460  915 VVREDRPgdlRLVAYVIPD 933
Cdd:cd17639   414 YADPDKS---YPVAIVVPN 429
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
 1537-2011 5.47e-20

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 96.87  E-value: 5.47e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1537 VEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGY--FI 1614
Cdd:PRK08279   43 FEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDV-----VALLMENRPEYLAAWLGLAKLGAVvaLL 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1615 -PVDPGYPMARLTRIADT----VTPALVLTRTGQGGCLPGAE---VFGDV---PVVALDRVADRLTAMPDQRPEVT--VD 1681
Cdd:PRK08279  118 nTQQRGAVLAHSLNLVDAkhliVGEELVEAFEEARADLARPPrlwVAGGDtldDPEGYEDLAAAAAGAPTTNPASRsgVT 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 PRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAAS---------------YPTAGrrGTLAHSSVgfdltmsalfepL 1746
Cdd:PRK08279  198 AKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLlrltpddvlycclplYHNTG--GTVAWSSV------------L 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1747 VSGRGVTLMP---ADATLADLAEElsGPLAYDYI-RLtpshLRHLVGHwtgqelPPAA-----RGWVVGGETLDPAL--- 1814
Cdd:PRK08279  264 AAGATLALRRkfsASRFWDDVRRY--RATAFQYIgEL----CRYLLNQ------PPKPtdrdhRLRLMIGNGLRPDIwde 331

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1815 ------VKQLLELrpdaevinhYGPTET------------VIGRVvhpvreagelavdsPLPLGRPLG------ETRLQV 1870
Cdd:PRK08279  332 fqqrfgIPRILEF---------YAASEGnvgfinvfnfdgTVGRV--------------PLWLAHPYAivkydvDTGEPV 388

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1871 LDA--WLEAVPVGAVGELF--IGGDGIARGYlGRPALTAEKFLPDpAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVK 1946
Cdd:PRK08279  389 RDAdgRCIKVKPGEVGLLIgrITDRGPFDGY-TDPEASEKKILRD-VFKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFR 466

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1947 LRGYRIELGEIEARMAEHPGVEQAVVLvrdqqlvGWFIP------------AEDHPPVTVSALRRFCAEQLPEFMVP 2011
Cdd:PRK08279  467 WKGENVATTEVENALSGFPGVEEAVVY-------GVEVPgtdgragmaaivLADGAEFDLAALAAHLYERLPAYAVP 536
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
 504-994 7.14e-20

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 96.62  E-value: 7.14e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  504 TPGAVAVI------EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPV 577
Cdd:cd05967    64 RGDQIALIydspvtGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAA 143

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  578 ERIAYLLSDARPVLVVT------------------DAATADRLGPDDIT----GLVVLEETDTGG--------YPATEPP 627
Cdd:cd05967   144 KELASRIDDAKPKLIVTascgiepgkvvpykplldKALELSGHKPHHVLvlnrPQVPADLTKPGRdldwsellAKAEPVD 223

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  628 AVP--AGHSAYVIYTSGSTGRPKGVVITRA----ALDnflAAMAERFPLTAEDRVLATTTVSFdIAG--LELYLPLRSGA 699
Cdd:cd05967   224 CVPvaATDPLYILYTSGTTGKPKGVVRDNGghavALN---WSMRNIYGIKPGDVWWAASDVGW-VVGhsYIVYGPLLHGA 299

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  700 GVVLADADTARNPAA-----LIDlagRHRVTLAQATPTLWQALVPE------LSGPALAGIRVL-VGGEALPAELARRLG 767
Cdd:cd05967   300 TTVLYEGKPVGTPDPgafwrVIE---KYQVNALFTAPTAIRAIRKEdpdgkyIKKYDLSSLRTLfLAGERLDPPTLEWAE 376

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  768 DA-GAEVTNMYGPTETTiWSTSGPTS---EDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAG---EGLARGYW 840
Cdd:cd05967   377 NTlGVPVIDHWWQTETG-WPITANPVglePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLplpPGCLLTLW 455

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  841 NRPGLTAEKFLPDpfgPPGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VRED 919
Cdd:cd05967   456 KNDERFKKLYLSK---FPG--YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVgVRDE 530

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  920 RPGDLRLVAYVI-PDGPVAPDALRTELSRtlpdyMIPAVIVPVPDF---------PTTPNGKLDRAALPAPDYGTRSTAR 989
Cdd:cd05967   531 LKGQVPLGLVVLkEGVKITAEELEKELVA-----LVREQIGPVAAFrlvifvkrlPKTRSGKILRRTLRKIADGEDYTIP 605


                  ....*
gi 653678460  990 PPRDD 994
Cdd:cd05967   606 STIED 610
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
 628-977 9.23e-20

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 96.04  E-value: 9.23e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  628 AVPAGHS--AYVIYTSGSTGRPKGVVITRAAL-DNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELY----------LP 694
Cdd:PRK12492  201 PVPVGLDdiAVLQYTGGTTGLAKGAMLTHGNLvANMLQVRACLSQLGPDGQPLMKEGQEVMIAPLPLYhiyaftancmCM 280

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  695 LRSGAGVVLADadTARNPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRVL-VGGEALPAELARRLGD-AG 770
Cdd:PRK12492  281 MVSGNHNVLIT--NPRDIPGFIKELGKWRFSALLGLNTLFVALMdhPGFKDLDFSALKLTnSGGTALVKATAERWEQlTG 358

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  771 AEVTNMYGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKF 850
Cdd:PRK12492  359 CTIVEGYGLTETSPVASTNPYGELA-RLGTVGIPVPGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAEAL 437

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  851 lpDPFGppgtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAY 929
Cdd:PRK12492  438 --DAEG-----WFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIgVPDERSGEAVKLFV 510

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460  930 VIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK12492  511 VARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRREL 558
E_NRPS cd19534
Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 1082-1494 9.74e-20

Epimerization domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Epimerization (E) domains of nonribosomal peptide synthetases (NRPS) flip the chirality of the end amino acid of a peptide being manufactured by the NRPS. E-domains are homologous to the Condensation (C) domains. NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. Specialized tailoring NRPS domains such as E-domains greatly increase the range of possible peptide products created by the NRPS machinery. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the E-domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity.


Pssm-ID: 380457 [Multi-domain]  Cd Length: 428  Bit Score: 94.63  E-value: 9.74e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQEsiWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMPVQVADPAGGGAAL 1161
Cdd:cd19534     1 EVPLTPIQR--WFFEQNLAGRHHFNQSVLLRVPQGLDPDALRQALRALVEHHDALRMRFRREDGGWQQRIRGDVEELFRL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1162 TERDAAPGTDLAQLLLAEAALGFTLATEH-PL-RAVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRDLTELYAADTGHRE 1239
Cdd:cd19534    79 EVVDLSSLAQAAAIEALAAEAQSSLDLEEgPLlAAALFDGTDGGDRLLLVIHHLVVDGVSWRILLEDLEAAYEQALAGEP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1240 PELdEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATEVAGDLPRPATpgggGGAVEFALAP---RQIqgIREL 1316
Cdd:cd19534   159 IPL-PSKTSFQTWAELLAEYAQSPALLEELAYWRELPAADYWGLPKDPEQTYGD----ARTVSFTLDEeetEAL--LQEA 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1317 ARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNPRLDnlvgcLVNTV---------VLrgDLSGAPTFHEL 1385
Cdd:cd19534   232 NAAYRTEINDLLLAALALAFQDWTGRAPPAIFLEGHGReeIDPGLD-----LSRTVgwftsmypvVL--DLEASEDLGDT 304

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1386 LRRT--------------------HARTADALAHQELP---FEHL-VADRGAGNGPLFRIMYSFSSQEPAGRSAgdvdle 1441
Cdd:cd19534   305 LKRVkeqlrripnkgigygilrylTPEGTKRLAFHPQPeisFNYLgQFDQGERDDALFVSAVGGGGSDIGPDTP------ 378

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|...
gi 653678460 1442 pvpvpvTTCKFDLVLTVVDGgsTLEGVLEYADDLYLPGTAERLVTSLTNLLAA 1494
Cdd:cd19534   379 ------RFALLDINAVVEGG--QLVITVSYSRNMYHEETIQQLADSYKEALEA 423
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
 1689-2028 1.09e-19

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 92.95  E-value: 1.09e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1689 IFTSGSTGQPKGVAVEHIGIVR-YLSWAAASYPTAGRRGTLAHS---SVGFDLTMSALFEplvsgRGVTLMP-----ADA 1759
Cdd:cd17638     6 MFTSGTTGRSKGVMCAHRQTLRaAAAWADCADLTEDDRYLIINPffhTFGYKAGIVACLL-----TGATVVPvavfdVDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1760 TLADLAEE----LSGPlaydyirltPSHLRHLVGHWTGQELPPAA-RGWVVGGETLDPALVKQLLELRPDAEVINHYGPT 1834
Cdd:cd17638    81 ILEAIEREritvLPGP---------PTLFQSLLDHPGRKKFDLSSlRAAVTGAATVPVELVRRMRSELGFETVLTAYGLT 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1835 ETVIGRVVHPvreaGELAVDSPLPLGRPLGETRLQVLDAwleavpvgavGELFIGGDGIARGYLGRPALTAEKFlpDPAG 1914
Cdd:cd17638   152 EAGVATMCRP----GDDAETVATTCGRACPGFEVRIADD----------GEVLVRGYNVMQGYLDDPEATAEAI--DADG 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1915 epgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQQL--VG-WFIPAEDHP 1990
Cdd:cd17638   216 -----WLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQvAVIGVPDERMgeVGkAFVVARPGV 290

                         330       340       350
                  ....*....|....*....|....*....|....*...
gi 653678460 1991 PVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd17638   291 TLTEEDVIAWCRERLANYKVPRFVRFLDELPRNASGKV 328
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
 1537-2028 1.85e-19

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 94.84  E-value: 1.85e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1537 VEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV 1616
Cdd:PRK07786   23 LARHALMQPDAPALRFLGNTTTWRELDDRVAALA-----GALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPV 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1617 DPGYPMARLTRIADTVTPALVLTRTGQGGClpGAEVFGDVP-----VVALDRVADRLTAMPDQRPEVTVDPRGL------ 1685
Cdd:PRK07786   98 NFRLTPPEIAFLVSDCGAHVVVTEAALAPV--ATAVRDIVPllstvVVAGGSSDDSVLGYEDLLAEAGPAHAPVdipnds 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1686 -AYSIFTSGSTGQPKGVAVEHIGIVrylswAAASYPTAGRRGTLAHSsVGFdlTMSALFE---------PLVSGRGVTLM 1755
Cdd:PRK07786  176 pALIMYTSGTTGRPKGAVLTHANLT-----GQAMTCLRTNGADINSD-VGF--VGVPLFHiagigsmlpGLLLGAPTVIY 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1756 PADA----TLADL--AEELSGplaydyIRLTPSHLRHLVGHWT--GQELPPAARGWvvGGETLDPALVKQLLELRPDAEV 1827
Cdd:PRK07786  248 PLGAfdpgQLLDVleAEKVTG------IFLVPAQWQAVCAEQQarPRDLALRVLSW--GAAPASDTLLRQMAATFPEAQI 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1828 INHYGPTEtvigrvVHPVREA--GELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTA 1905
Cdd:PRK07786  320 LAAFGQTE------MSPVTCMllGEDAIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATA 393

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1906 EKFlpdpAGEpgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQL-----V 1980
Cdd:PRK07786  394 EAF----AGG----WFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKwgevpV 465

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460 1981 GWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PRK07786  466 AVAAVRNDDAALTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKV 513
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
 507-977 2.30e-19

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 94.31  E-value: 2.30e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  507 AVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSD 586
Cdd:PRK13390   15 AVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGD 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  587 ARPVLVVTDAA---TADRLGPD---------DITGLVVLEETDTG-GYPATEPPAvpaghSAYVIYTSGSTGRPKGVvit 653
Cdd:PRK13390   95 SGARVLVASAAldgLAAKVGADlplrlsfggEIDGFGSFEAALAGaGPRLTEQPC-----GAVMLYSSGTTGFPKGI--- 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  654 raaldnfLAAMAERFPLTAEDRVLATTTVSFDIAGLELYL---------PLR-------SGAGVVLADADTARNPAALID 717
Cdd:PRK13390  167 -------QPDLPGRDVDAPGDPIVAIARAFYDISESDIYYssapiyhaaPLRwcsmvhaLGGTVVLAKRFDAQATLGHVE 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  718 lagRHRVTLAQATPTLWQALV-------PELSGPALAGirVLVGGEALPAELARRLGD-AGAEVTNMYGPTEttiwsTSG 789
Cdd:PRK13390  240 ---RYRITVTQMVPTMFVRLLkldadvrTRYDVSSLRA--VIHAAAPCPVDVKHAMIDwLGPIVYEYYSSTE-----AHG 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSEDS----IRRGSIGVPIDNTqVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLP-DPFgppgtrmyr 864
Cdd:PRK13390  310 MTFIDSpdwlAHPGSVGRSVLGD-LHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPaHPF--------- 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  865 tgdlvrWSAAGDLEYL---------GRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV------VREDRPGDLRLVAY 929
Cdd:PRK13390  380 ------WTTVGDLGSVdedgylylaDRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIgvpdpeMGEQVKAVIQLVEG 453

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460  930 VIPDGPVAPDALRTELSRtLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK13390  454 IRGSDELARELIDYTRSR-IAHYKAPRSVEFVDELPRTPTGKLVKGLL 500
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
1534-2029 3.64e-19

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 94.04  E-value: 3.64e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1534 HQLVEAQADRTpgWTALRTGdRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:PRK06087   30 QQTARAMPDKI--AVVDNHG-ASYTYSALDHAASRLA-----NWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVS 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPMARLTRIADTV------TPALVLTRTGQGGCLPGAEvfgDVP----VVALDRVADRLTA------MPDQRP- 1676
Cdd:PRK06087  102 VPLLPSWREAELVWVLNKCqakmffAPTLFKQTRPVDLILPLQN---QLPqlqqIVGVDKLAPATSSlslsqiIADYEPl 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1677 --EVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVrylswAAASYPTAGRRGT----------LAHSSvGFDLTMSALFe 1744
Cdd:PRK06087  179 ttAITTHGDELAAVLFTSGTEGLPKGVMLTHNNIL-----ASERAYCARLNLTwqdvfmmpapLGHAT-GFLHGVTAPF- 251

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1745 pLVSGRGVTL--MPADATLADLAEE----LSG--PLAYDYIRLTPSHLRHLvghwtgqelpPAARGWVVGGETLDPALVK 1816
Cdd:PRK06087  252 -LIGARSVLLdiFTPDACLALLEQQrctcMLGatPFIYDLLNLLEKQPADL----------SALRFFLCGGTTIPKKVAR 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1817 QLLElrPDAEVINHYGPTETVIGRVVHPvreagelavDSPLPL-----GRPLGETRLQVLDAWLEAVPVGAVGELFIGGD 1891
Cdd:PRK06087  321 ECQQ--RGIKLLSVYGSTESSPHAVVNL---------DDPLSRfmhtdGYAAAGVEIKVVDEARKTLPPGCEGEEASRGP 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1892 GIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQA 1970
Cdd:PRK06087  390 NVFMGYLDEPELTARAL--DEEG-----WYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIhDAC 462

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1971 VVLVRDQQL----VGWFIPAEDHPPVTVSALRRFCAEQ-LPEFMVPNQWVALDAFPLTPHGKVN 2029
Cdd:PRK06087  463 VVAMPDERLgersCAYVVLKAPHHSLTLEEVVAFFSRKrVAKYKYPEHIVVIDKLPRTASGKIQ 526
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
 1554-2011 4.38e-19

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 92.80  E-value: 4.38e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1554 DRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGgyfipvdpgypmarltriadtVT 1633
Cdd:cd05940     1 DEALTYAELDAMANRYARWLK--SLG---LKPGDVVALFMENRPEYVLLWLGLVKIG---------------------AV 54

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1634 PALV-LTRTGQggclpgaevfgdvpvvALDRVADRLTAMpdqrpEVTVDPrglAYSIFTSGSTGQPKGVAVEHIGIVRYL 1712
Cdd:cd05940    55 AALInYNLRGE----------------SLAHCLNVSSAK-----HLVVDA---ALYIYTSGTTGLPKAAIISHRRAWRGG 110

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1713 SWAAASyptagrrGTLAHSSVGFDlTMsalfePLVSGRGVTLMP-----ADATLAdLAEELSGPLAYDYIRLTPSHLRHL 1787
Cdd:cd05940   111 AFFAGS-------GGALPSDVLYT-CL-----PLYHSTALIVGWsaclaSGATLV-IRKKFSASNFWDDIRKYQATIFQY 176

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1788 VGhwtgqEL-------PP-----AARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETV------------IGRVVH 1843
Cdd:cd05940   177 IG-----ELcryllnqPPkpterKHKVRMIFGNGLRPDIWEEFKERFGVPRIAEFYAATEGNsgfinffgkpgaIGRNPS 251

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1844 PVREAGELA-VDSPLPLGRPLGETrlqvlDAWLEAVPVGAVGELF--IGGDGIARGYLGrPALTAEKFLPDpAGEPGARM 1920
Cdd:cd05940   252 LLRKVAPLAlVKYDLESGEPIRDA-----EGRCIKVPRGEPGLLIsrINPLEPFDGYTD-PAATEKKILRD-VFKKGDAW 324

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1921 YRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLvrdqqlvGWFIPAED------------ 1988
Cdd:cd05940   325 FNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVY-------GVQVPGTDgragmaaivlqp 397

                         490       500
                  ....*....|....*....|...
gi 653678460 1989 HPPVTVSALRRFCAEQLPEFMVP 2011
Cdd:cd05940   398 NEEFDLSALAAHLEKNLPGYARP 420
PLN02246 PLN02246
4-coumarate--CoA ligase
 495-979 4.54e-19

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 93.51  E-value: 4.54e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  495 ERFQQWcrrtPGAVAVIEGDT--TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPID 572
Cdd:PLN02246   31 ERLSEF----SDRPCLIDGATgrVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTAN 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  573 PDFPVERIAYLLSDARPVLVVTDAATADRL----------------GPDDITGLVVLEETDTGGYPATE-----PPAVPa 631
Cdd:PLN02246  107 PFYTPAEIAKQAKASGAKLIITQSCYVDKLkglaeddgvtvvtiddPPEGCLHFSELTQADENELPEVEispddVVALP- 185

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  632 ghsayviYTSGSTGRPKGVVITRAALDNFLAAMAE----RFPLTAEDRVLATTTVsFDIAGLE--LYLPLRSGAGVVL-A 704
Cdd:PLN02246  186 -------YSSGTTGLPKGVMLTHKGLVTSVAQQVDgenpNLYFHSDDVILCVLPM-FHIYSLNsvLLCGLRVGAAILImP 257

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  705 DADTarnpAALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIRVLVGGealPAELARRLGDA-GAEVTNM----- 776
Cdd:PLN02246  258 KFEI----GALLELIQRHKVTIAPFVPPIVLAIAksPVVEKYDLSSIRMVLSG---AAPLGKELEDAfRAKLPNAvlgqg 330

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  777 YGPTEttiwstSGPTSEDS---------IRRGSIGVPIDNTQVYVLD----ANLhpaPIGVLGELHIAGEGLARGYWNRP 843
Cdd:PLN02246  331 YGMTE------AGPVLAMClafakepfpVKSGSCGTVVRNAELKIVDpetgASL---PRNQPGEICIRGPQIMKGYLNDP 401

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  844 GLTAekflpdpfgppgtrmyRTGDLVRWSAAGDLEYLGRTDHQ---------VKLRGFRIELGEIETTLINAGPVRRAAA 914
Cdd:PLN02246  402 EATA----------------NTIDKDGWLHTGDIGYIDDDDELfivdrlkelIKYKGFQVAPAELEALLISHPSIADAAV 465

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460  915 VVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTLPDY-MIPAVIVpVPDFPTTPNGKLDRAALPA 979
Cdd:PLN02246  466 VPMKDEVAGEVPVAFVVrsNGSEITEDEIKQFVAKQVVFYkRIHKVFF-VDSIPKAPSGKILRKDLRA 532
PRK07788 PRK07788
acyl-CoA synthetase; Validated
 1536-2035 7.28e-19

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 93.07  E-value: 7.28e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIP 1615
Cdd:PRK07788   54 LVAHAARRAPDRAALIDERGTLTYAELDEQSNALARGL--LALG---VRAGDGVAVLARNHRGFVLALYAAGKVGARIIL 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1616 VDPGYPMARLTRIA-----------DTVTPALVLTRTGQGGCLP-GAEVFGDVPVVALDRVADRLTAMPDQRPEVTVDPR 1683
Cdd:PRK07788  129 LNTGFSGPQLAEVAaregvkalvydDEFTDLLSALPPDLGRLRAwGGNPDDDEPSGSTDETLDDLIAGSSTAPLPKPPKP 208

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1684 GlAYSIFTSGSTGQPKGVAVEHI-------GIVRYLSW--------AAASYPTAGrrgtLAHSSVGFDL----TMSALFE 1744
Cdd:PRK07788  209 G-GIVILTSGTTGTPKGAPRPEPsplaplaGLLSRVPFragettllPAPMFHATG----WAHLTLAMALgstvVLRRRFD 283

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1745 PlvsgrgvtlmpaDATLADLAEELSgplayDYIRLTPSHLRHLVghwtgqELPPAARG---------WVVGGETLDPALV 1815
Cdd:PRK07788  284 P------------EATLEDIAKHKA-----TALVVVPVMLSRIL------DLGPEVLAkydtsslkiIFVSGSALSPELA 340

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1816 KQLLELRpdAEVI-NHYGPTETVIGRVVHPvreaGELAVDsPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIA 1894
Cdd:PRK07788  341 TRALEAF--GPVLyNLYGSTEVAFATIATP----EDLAEA-PGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPF 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1895 RGYLGRPaltaekflpDPAGEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVL 1973
Cdd:PRK07788  414 EGYTDGR---------DKQIIDG--LLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVvEAAVIG 482

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1974 VRD----QQLVGWFIPAEDHPpVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPG 2035
Cdd:PRK07788  483 VDDeefgQRLRAFVVKAPGAA-LDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELRE 547
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
 1530-2028 1.05e-18

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 92.53  E-value: 1.05e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTAL--RTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVL 1607
Cdd:PRK12583   17 TQTIGDAFDATVARFPDREALvvRHQALRYTWRQLADAVDRLARGLL--ALG---VQPGDRVGIWAPNCAEWLLTQFATA 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1608 KAGGYFIPVDPGYPMARLT-----------------------RIADTVTPALVLTRTGQGGC-----LPGAEVFGDVP-- 1657
Cdd:PRK12583   92 RIGAILVNINPAYRASELEyalgqsgvrwvicadafktsdyhAMLQELLPGLAEGQPGALACerlpeLRGVVSLAPAPpp 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1658 -VVALDRVADRLTAMPDQR-PEVTV-----DPRGLAYsifTSGSTGQPKGVAVEHIGIVRylswaaASYPTAGRRGTLAH 1730
Cdd:PRK12583  172 gFLAWHELQARGETVSREAlAERQAsldrdDPINIQY---TSGTTGFPKGATLSHHNILN------NGYFVAESLGLTEH 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1731 SSV--------GFDLTMSALFepLVSGRGVTLMPAD-----ATLADLAEELSGPLaYDYIRLTPSHLRH-LVGHWTGQEL 1796
Cdd:PRK12583  243 DRLcvpvplyhCFGMVLANLG--CMTVGACLVYPNEafdplATLQAVEEERCTAL-YGVPTMFIAELDHpQRGNFDLSSL 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1797 ppaaRGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETvigrvvHPVreAGELAVDSPLPL-----GRPLGETRLQVL 1871
Cdd:PRK12583  320 ----RTGIMAGAPCPIEVMRRVMDEMHMAEVQIAYGMTET------SPV--SLQTTAADDLERrvetvGRTQPHLEVKVV 387

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1872 DAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDpagepgARMYrTGDLVRWRGDGLLDFVGRVDDQVKLRGYR 1951
Cdd:PRK12583  388 DPDGATVPRGEIGELCTRGYSVMKGYWNNPEATAESIDED------GWMH-TGDLATMDEQGYVRIVGRSKDMIIRGGEN 460

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1952 IELGEIEARMAEHPGVEQ-AVVLVRDQ----QLVGWFIPAEDHpPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHG 2026
Cdd:PRK12583  461 IYPREIEEFLFTHPAVADvQVFGVPDEkygeEIVAWVRLHPGH-AASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTG 539


                  ..
gi 653678460 2027 KV 2028
Cdd:PRK12583  540 KV 541
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
 1531-2033 1.14e-18

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 92.05  E-value: 1.14e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1531 RTVHQLVEAQADRTPGWTAL----RTGD-RSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLA 1605
Cdd:PRK08008    7 QHLRQMWDDLADVYGHKTALifesSGGVvRRYSYLELNEEINRTANLFYS--LG---IRKGDKVALHLDNCPEFIFCWFG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1606 VLKAGGYFIPVDPGY------------------------PM-ARLTRIADTVTPALVLTRTGQGGclpgaevfgDVPVVA 1660
Cdd:PRK08008   82 LAKIGAIMVPINARLlreesawilqnsqasllvtsaqfyPMyRQIQQEDATPLRHICLTRVALPA---------DDGVSS 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1661 LDRVADRLTAMPDQRPEVTVDprGLAYSIFTSGSTGQPKGVAVEHIGIV---RYLSWAAAsyptagrrgtLAHSSVGfdL 1737
Cdd:PRK08008  153 FTQLKAQQPATLCYAPPLSTD--DTAEILFTSGTTSRPKGVVITHYNLRfagYYSAWQCA----------LRDDDVY--L 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1738 TMSALFEplVSGRGVTLMPA---DATLAdLAEELSGPLAYDYIR-----LT---PSHLRHLVG----------------- 1789
Cdd:PRK08008  219 TVMPAFH--IDCQCTAAMAAfsaGATFV-LLEKYSARAFWGQVCkyratITeciPMMIRTLMVqppsandrqhclrevmf 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1790 --HWTGQElppaargwvvggetldpalvKQLLELRPDAEVINHYGPTETVIGrvvhpvreageLAVDSP-----LP-LGR 1861
Cdd:PRK08008  296 ylNLSDQE--------------------KDAFEERFGVRLLTSYGMTETIVG-----------IIGDRPgdkrrWPsIGR 344

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1862 PLGETRLQVLDAWLEAVPVGAVGELFIGG---DGIARGYLGRPALTAEKFlpdpagEPGARMYrTGDLVRWRGDGLLDFV 1938
Cdd:PRK08008  345 PGFCYEAEIRDDHNRPLPAGEIGEICIKGvpgKTIFKEYYLDPKATAKVL------EADGWLH-TGDTGYVDEEGFFYFV 417

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-----VRDQQLVGWFIPAEDHPpVTVSALRRFCAEQLPEFMVPNQ 2013
Cdd:PRK08008  418 DRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVgikdsIRDEAIKAFVVLNEGET-LSEEEFFAFCEQNMAKFKVPSY 496

                         570       580
                  ....*....|....*....|
gi 653678460 2014 WVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK08008  497 LEIRKDLPRNCSGKIIKKNL 516
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
 501-971 1.28e-18

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 92.72  E-value: 1.28e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  501 CRRTPGAVAVIEGD----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFP 576
Cdd:cd05943    79 HADADDPAAIYAAEdgerTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFG 158

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  577 V-------------------------------ERIAYL---LSDARPVLVVTDAATADRLGPDDITGLVVLEE---TDTG 619
Cdd:cd05943   159 VpgvldrfgqiepkvlfavdaytyngkrhdvrEKVAELvkgLPSLLAVVVVPYTVAAGQPDLSKIAKALTLEDflaTGAA 238

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  620 GYPATEPpaVPAGHSAYVIYTSGSTGRPKGVVitRAALDNFLAAMAE---RFPLTAEDRVLATTTVS-----FDIAGLEl 691
Cdd:cd05943   239 GELEFEP--LPFDHPLYILYSSGTTGLPKCIV--HGAGGTLLQHLKEhilHCDLRPGDRLFYYTTCGwmmwnWLVSGLA- 313

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  692 ylplrSGAGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQALvpELSG--PA----LAGIR-VLVGGEALPAELA 763
Cdd:cd05943   314 -----VGATIVLYDgSPFYPDTNALWDLADEEGITVFGTSAKYLDAL--EKAGlkPAethdLSSLRtILSTGSPLKPESF 386

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  764 RRLGDAGAEvtnmygptetTIW--STSGPT----------SEDSIRRGSIGVPIDNTQVYVLDANLHPApIGVLGELHIA 831
Cdd:cd05943   387 DYVYDHIKP----------DVLlaSISGGTdiiscfvggnPLLPVYRGEIQCRGLGMAVEAFDEEGKPV-WGEKGELVCT 455

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 GEGLAR--GYWNRPGltAEKFLPDPFGP-PGTrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGP 908
Cdd:cd05943   456 KPFPSMpvGFWNDPD--GSRYRAAYFAKyPGV--WAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPE 531

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460  909 VRRAAAVVREDRPGDLRLVAYV-IPDGPVAPDALRTELSRTLPDYM----IPAVIVPVPDFPTTPNGK 971
Cdd:cd05943   532 VEDSLVVGQEWKDGDERVILFVkLREGVELDDELRKRIRSTIRSALsprhVPAKIIAVPDIPRTLSGK 599
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
 1690-2028 1.98e-18

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 89.00  E-value: 1.98e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 FTSGSTGQPKGVAVEHigivryLSWAAaSYPtAGRR-------------GTLAHSsvgfdLTMSALFEPLVSGRGVTLMP 1756
Cdd:cd17633     7 FTSGTTGLPKAYYRSE------RSWIE-SFV-CNEDlfnisgedailapGPLSHS-----LFLYGAISALYLGGTFIGQR 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1757 --ADATLADLAEELSGPLAYdyirLTPSHLRHLVGHwtgQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPT 1834
Cdd:cd17633    74 kfNPKSWIRKINQYNATVIY----LVPTMLQALART---LEPESKIKSIFSSGQKLFESTKKKLKNIFPKANLIEFYGTS 146

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1835 ET--VIGRVVhpvreaGELAvdSPLPLGRPLGETRLQVLDAwleavPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdp 1912
Cdd:cd17633   147 ELsfITYNFN------QESR--PPNSVGRPFPNVEIEIRNA-----DGGEIGKIFVKSEMVFSGYVRGGFSNPDGW---- 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1913 agepgarmYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL----VRDQQLVGWFIPAED 1988
Cdd:cd17633   210 --------MSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVgipdARFGEIAVALYSGDK 281

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 653678460 1989 hppVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd17633   282 ---LTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
 1678-2033 2.05e-18

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 91.24  E-value: 2.05e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1678 VTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTLA-----HSsvgFDLTmSALFEPLVSGRGV 1752
Cdd:cd05909   142 APVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGalpffHS---FGLT-GCLWLPLLSGIKV 217

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1753 TLMPADatladLAEELSGPLAYDY---IRL-TPSHLRHLVGHWTGQELPpAARGWVVGGETLDPALvKQLLELRPDAEVI 1828
Cdd:cd05909   218 VFHPNP-----LDYKKIPELIYDKkatILLgTPTFLRGYARAAHPEDFS-SLRLVVAGAEKLKDTL-RQEFQEKFGIRIL 290

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1829 NHYGPTETVigrvvhPVreageLAVDSPLP------LGRPL--GETRLqVLDAWLEAVPVGAVGELFIGGDGIARGYLGR 1900
Cdd:cd05909   291 EGYGTTECS------PV-----ISVNTPQSpnkegtVGRPLpgMEVKI-VSVETHEEVPIGEGGLLLVRGPNVMLGYLNE 358

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1901 PALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPG--VEQAVVLVRDQQ 1978
Cdd:cd05909   359 PELTSFAF--------GDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILPedNEVAVVSVPDGR 430

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1979 lvgwfiPAE------DHPPVTVSALRRFCAE-QLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05909   431 ------KGEkivlltTTTDTDPSSLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTL 486
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
 1555-1973 2.64e-18

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 90.35  E-value: 2.64e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1555 RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGyfIPVdPGYPmarltriadTVTP 1634
Cdd:cd05907     4 QPITWAEFAEEVRALAKGLI--ALG---VEPGDRVAILSRNRPEWTIADLAILAIGA--VPV-PIYP---------TSSA 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1635 ALVltrtgqggclpgAEVFGDVPVVALdrvadrltampdqrpeVTVDPRGLAYSIFTSGSTGQPKGVAVEHigivRYLSW 1714
Cdd:cd05907    67 EQI------------AYILNDSEAKAL----------------FVEDPDDLATIIYTSGTTGRPKGVMLSH----RNILS 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1715 AAASYPTAGRRGTLAHsSVGFdLTMSALFE-------PLVSGRGVTLMPADATL-ADLAEE-----LSGPLAYDYIRLTP 1781
Cdd:cd05907   115 NALALAERLPATEGDR-HLSF-LPLAHVFErraglyvPLLAGARIYFASSAETLlDDLSEVrptvfLAVPRVWEKVYAAI 192

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1782 SH-----LRHLVGHWT-GQELppaaRGWVVGGETLDPALVKQLLELrpDAEVINHYGPTETviGRVVHpVREAGELAVDS 1855
Cdd:cd05907   193 KVkavpgLKRKLFDLAvGGRL----RFAASGGAPLPAELLHFFRAL--GIPVYEGYGLTET--SAVVT-LNPPGDNRIGT 263

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1856 PlplGRPLGETRLQvldawleavpVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAgepgarmYRTGDLVRWRGDGLL 1935
Cdd:cd05907   264 V---GKPLPGVEVR----------IADDGEILVRGPNVMLGYYKNPEATAEALDADGW-------LHTGDLGEIDEDGFL 323

                         410       420       430
                  ....*....|....*....|....*....|....*....
gi 653678460 1936 DFVGRVDDQVKLR-GYRIELGEIEARMAEHPGVEQAVVL 1973
Cdd:cd05907   324 HITGRKKDLIITSgGKNISPEPIENALKASPLISQAVVI 362
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
 620-977 8.89e-18

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 89.82  E-value: 8.89e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  620 GYPATEppAVPAGHSAYVI-YTSGSTGRPKGVVITRAaldNFLAAMAERFPLTAEDRVLATTTVsfdIAGLELY----LP 694
Cdd:PRK05677  196 GQPVTE--ANPQADDVAVLqYTGGTTGVAKGAMLTHR---NLVANMLQCRALMGSNLNEGCEIL---IAPLPLYhiyaFT 267

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  695 LRSGAGVVLADAD----TARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGPAL--AGIRV-LVGGEALPAELARRLG 767
Cdd:PRK05677  268 FHCMAMMLIGNHNilisNPRDLPAMVKELGKWKFSGFVGLNTLFVALCNNEAFRKLdfSALKLtLSGGMALQLATAERWK 347

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  768 D-AGAEVTNMYGPTETTIWSTSGPTseDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLT 846
Cdd:PRK05677  348 EvTGCAICEGYGMTETSPVVSVNPS--QAIQVGTIGIPVPSTLCKVIDDDGNELPLGEVGELCVKGPQVMKGYWQRPEAT 425

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  847 AEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLR 925
Cdd:PRK05677  426 DEILDSDGW-------LKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIgVPDEKSGEAI 498

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|...
gi 653678460  926 LVAYVIPDGP-VAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK05677  499 KVFVVVKPGEtLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
 1827-2033 8.92e-18

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 89.50  E-value: 8.92e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1827 VINHYGPTETVIGRVVHPVREAGELAVdsplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAE 1906
Cdd:PRK12492  361 IVEGYGLTETSPVASTNPYGELARLGT-----VGIPVPGTALKVIDDDGNELPLGERGELCIKGPQVMKGYWQQPEATAE 435

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1907 KFlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQ---QLVGW 1982
Cdd:PRK12492  436 AL--DAEG-----WFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANcAAIGVPDErsgEAVKL 508

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1983 FIPAEDhPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK12492  509 FVVARD-PGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRREL 558
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
1540-2033 9.27e-18

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 89.25  E-value: 9.27e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1540 QADRTPGWTALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAV--LKAGGYFIPVd 1617
Cdd:PRK03640   11 RAFLTPDRTAIEFEEKKVTFMELHEAVVSVA-----GKLAALGVKKGDRVALLMKNGMEMILVIHALqqLGAVAVLLNT- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 pgypmaRLTR------IADT-VTpaLVLTRTGQGgclpgAEVFGDVPVVaLDRVADrlTAMPDQRPEVTVDPRGLAYSIF 1690
Cdd:PRK03640   85 ------RLSReellwqLDDAeVK--CLITDDDFE-----AKLIPGISVK-FAELMN--GPKEEAEIQEEFDLDEVATIMY 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1691 TSGSTGQPKGV-------------AVEHIGIVRYLSWAAAsYPtagrrgtLAHSSvGFdltmSALFEPLVSGRGVTLMPA 1757
Cdd:PRK03640  149 TSGTTGKPKGViqtygnhwwsavgSALNLGLTEDDCWLAA-VP-------IFHIS-GL----SILMRSVIYGMRVVLVEK 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1758 -DATLA-DLAEELSGPLaydyIRLTPSHLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLElrPDAEVINHYGPTE 1835
Cdd:PRK03640  216 fDAEKInKLLQTGGVTI----ISVVSTMLQRLLERLGEGTYPSSFRCMLLGGGPAPKPLLEQCKE--KGIPVYQSYGMTE 289

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 TV--IgrvvhpVREAGELAVDSPLPLGRPLGETRLQVLDAWLEAVPvGAVGELFIGGDGIARGYLGRPALTAEKFlpdpa 1913
Cdd:PRK03640  290 TAsqI------VTLSPEDALTKLGSAGKPLFPCELKIEKDGVVVPP-FEEGEIVVKGPNVTKGYLNREDATRETF----- 357

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1914 gEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQlvgW------FIPA 1986
Cdd:PRK03640  358 -QDG--WFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVgVPDDK---WgqvpvaFVVK 431

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*..
gi 653678460 1987 EDhpPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK03640  432 SG--EVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHEL 476
PRK05691 PRK05691
peptide synthase; Validated
 1532-2122 1.33e-17

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 90.61  E-value: 1.33e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALR------TGDRSLTFAGLDAQANRLAHALHTGAlgappvGPETPVLLLLDRSPELVVAMLA 1605
Cdd:PRK05691   10 TLVQALQRRAAQTPDRLALRfladdpGEGVVLSYRDLDLRARTIAAALQARA------SFGDRAVLLFPSGPDYVAAFFG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1606 VLKAGgyFIPVdPGYP--------MARLTRIADTVTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLTAMPDQRPE 1677
Cdd:PRK05691   84 CLYAG--VIAV-PAYPpesarrhhQERLLSIIADAEPRLLLTVADLRDSLLQMEELAAANAPELLCVDTLDPALAEAWQE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1678 VTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRylswaaasyptagrRGTLAHSSVGFDLT-----------------MS 1740
Cdd:PRK05691  161 PALQPDDIAFLQYTSGSTALPKGVQVSHGNLVA--------------NEQLIRHGFGIDLNpddvivswlplyhdmglIG 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1741 ALFEPLVSGRGVTLMPADATLADLAEELS-----------GP-LAYDYI--RLTPSHLRHLvghwtgqELppaaRGWVV- 1805
Cdd:PRK05691  227 GLLQPIFSGVPCVLMSPAYFLERPLRWLEaiseyggtisgGPdFAYRLCseRVSESALERL-------DL----SRWRVa 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1806 --GGETLDPALVKQLLE------LRPDAeVINHYGPTETVI-------GRVVHPVR-EAGELAVDSPLP--------LGR 1861
Cdd:PRK05691  296 ysGSEPIRQDSLERFAEkfaacgFDPDS-FFASYGLAEATLfvsggrrGQGIPALElDAEALARNRAEPgtgsvlmsCGR 374

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1862 PLGETRLQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPdpagEPGARMYRTGDLVRWRgDGLLDFVGR 1940
Cdd:PRK05691  375 SQPGHAVLIVDpQSLEVLGDNRVGEIWASGPSIAHGYWRNPEASAKTFVE----HDGRTWLRTGDLGFLR-DGELFVTGR 449

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1941 VDDQVKLRGYRIELGEIEARMAEHPGVEQ----AVVLVRDQQLVGWFIPAEDH--------PPVTVSALRRFCAEQLPEf 2008
Cdd:PRK05691  450 LKDMLIVRGHNLYPQDIEKTVEREVEVVRkgrvAAFAVNHQGEEGIGIAAEISrsvqkilpPQALIKSIRQAVAEACQE- 528

                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2009 mVPNQWVALD--AFPLTPHGKVNHRA---------------LPGptsGRPELEDRYQAPRTPGEQLLAELWSTVLGTDRI 2071
Cdd:PRK05691  529 -APSVVLLLNpgALPKTSSGKLQRSAcrlrladgsldsyalFPA---LQAVEAAQTAASGDELQARIAAIWCEQLKVEQV 604

                         650       660       670       680       690
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460 2072 GIEDNFFDLGGTSISVIQLSGACR-RAGVEISPKDVFEQPTVRGQAMrAIAR 2122
Cdd:PRK05691  605 AADDHFFLLGGNSIAATQVVARLRdELGIDLNLRQLFEAPTLAAFSA-AVAR 655
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
1589-2055 1.65e-17

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 89.32  E-value: 1.65e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1589 VLLLLDRSPELVVAMLAVLKAGGYFIPVDP-------GYPmARLTRIADTVTPALVLTRTGQGGCLPGAEVFGDvpvval 1661
Cdd:PRK06060   58 VLLCLPDSPDLVQLLLACLARGVMAFLANPelhrddhALA-ARNTEPALVVTSDALRDRFQPSRVAEAAELMSE------ 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1662 drvADRltAMPDQRPEVTVDprGLAYSIFTSGSTGQPKGVAVEH---IGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLT 1738
Cdd:PRK06060  131 ---AAR--VAPGGYEPMGGD--ALAYATYTSGTTGPPKAAIHRHadpLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLG 203

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1739 MSALFePLVSGRGVTLMPADATlADLAEELSGPLAYDYIRLTPSHLRHLVGHWTGQELPpAARGWVVGGETLDPALVKQL 1818
Cdd:PRK06060  204 NSVWF-PLATGGSAVINSAPVT-PEAAAILSARFGPSVLYGVPNFFARVIDSCSPDSFR-SLRCVVSAGEALELGLAERL 280

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1819 LELRPDAEVINHYGPTETVIGRVVHPVREAgelavdSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYL 1898
Cdd:PRK06060  281 MEFFGGIPILDGIGSTEVGQTFVSNRVDEW------RLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYW 354

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1899 GRPaltaekflpDPAGEPGARMyRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD- 1976
Cdd:PRK06060  355 NRP---------DSPVANEGWL-DTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVaEAAVVAVREs 424

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1977 ---QQLVGWFIPAEDHPpVTVSALR---RFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPTSGRP--ELEDRYQ 2048
Cdd:PRK06060  425 tgaSTLQAFLVATSGAT-IDGSVMRdlhRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALRKQSPTKPiwELSLTEP 503


                  ....*..
gi 653678460 2049 APRTPGE 2055
Cdd:PRK06060  504 GSGVRAQ 510
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
 1548-2033 2.13e-17

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 88.41  E-value: 2.13e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALR----TGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY-PM 1622
Cdd:PRK04319   61 VALRyldaSRKEKYTYKELKELSNKFANVLKE--LG---VEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFmEE 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1623 ARLTRIADT------VTPALvLTRTGQGGcLP--------GAEVFGDVPVVALDRvadRLTAMPDQRPEVTVDPRGLAYS 1688
Cdd:PRK04319  136 AVRDRLEDSeakvliTTPAL-LERKPADD-LPslkhvllvGEDVEEGPGTLDFNA---LMEQASDEFDIEWTDREDGAIL 210

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1689 IFTSGSTGQPKGVAVEHIGIVRYlsWAAASYP-----------TAGrRGTLAHSSVGfdltmsaLFEPLVSGrgvtlmpa 1757
Cdd:PRK04319  211 HYTSGSTGKPKGVLHVHNAMLQH--YQTGKYVldlheddvywcTAD-PGWVTGTSYG-------IFAPWLNG-------- 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1758 dATLADLAEELSGPLAYDYI---RLT-----PSHLRHLVGHwtGQELP-----PAARGWVVGGETLDPALV---KQLLEL 1821
Cdd:PRK04319  273 -ATNVIDGGRFSPERWYRILedyKVTvwytaPTAIRMLMGA--GDDLVkkydlSSLRHILSVGEPLNPEVVrwgMKVFGL 349

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1822 RpdaeVINHYGPTET---VIgrvvhpvreAGELAVD-SPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFI--GGDGIAR 1895
Cdd:PRK04319  350 P----IHDNWWMTETggiMI---------ANYPAMDiKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIkkGWPSMMR 416

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1896 GYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR 1975
Cdd:PRK04319  417 GIWNNPEKYESYFAGD--------WYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGK 488

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460 1976 DQQLVGWFIPA-----EDHPPvtVSALRR----FCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK04319  489 PDPVRGEIIKAfvalrPGYEP--SEELKEeirgFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVL 553
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
 1553-2033 2.49e-17

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 88.07  E-value: 2.49e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1553 GDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIAD-- 1630
Cdd:cd12119    22 EVHRYTYAEVAERARRLANALR--RLG---VKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINha 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1631 -----TVTPALVLTRTGQGGCLPGAEVFgdvpVVALDRVADRLTAMP---------DQRPEVTVDPR---GLAYSI-FTS 1692
Cdd:cd12119    97 edrvvFVDRDFLPLLEAIAPRLPTVEHV----VVMTDDAAMPEPAGVgvlayeellAAESPEYDWPDfdeNTAAAIcYTS 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1693 GSTGQPKGVAVEHIGIVRYlSWAAASYPTAGrrgtLAHSSVGfdLTMSALFEplVSGRGvtlMPADATLADLAEELSGPl 1772
Cdd:cd12119   173 GTTGNPKGVVYSHRSLVLH-AMAALLTDGLG----LSESDVV--LPVVPMFH--VNAWG---LPYAAAMVGAKLVLPGP- 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 aydyiRLTPSHLRHLV---------------------GHWTGQELPPAaRGWVVGGETLDPALVKQLLELrpDAEVINHY 1831
Cdd:cd12119   240 -----YLDPASLAELIeregvtfaagvptvwqglldhLEANGRDLSSL-RRVVIGGSAVPRSLIEAFEER--GVRVIHAW 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETV-IGRVVHPVREAGELAVDSPLPL----GRPLGETRLQVLDAWLEAVPV--GAVGELFIGGDGIARGYLGRPALT 1904
Cdd:cd12119   312 GMTETSpLGTVARPPSEHSNLSEDEQLALrakqGRPVPGVELRIVDDDGRELPWdgKAVGELQVRGPWVTKSYYKNDEES 391

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1905 AEKFlpdpagEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQQL---- 1979
Cdd:cd12119   392 EALT------EDG--WLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEaAVIGVPHPKWgerp 463

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1980 VGWFIPAEDHPpVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd12119   464 LAVVVLKEGAT-VTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
515-977 3.22e-17

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 88.01  E-value: 3.22e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLAR-LLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDA-RPVLV 592
Cdd:PRK08751   49 KTITYREADQLVEQFAAyLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSgASVLV 128

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  593 VTD----------AAT----------ADRLG------------------PD-DITGLVVLEETDTGGYPATEPPA-VPAG 632
Cdd:PRK08751  129 VIDnfgttvqqviADTpvkqvittglGDMLGfpkaalvnfvvkyvkklvPEyRINGAIRFREALALGRKHSMPTLqIEPD 208

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  633 HSAYVIYTSGSTGRPKGVVITRAaldNFLAAMAERFP-LTAEDRVLATTTVSfdIAGLELYLPLRSGA-GVVLADA---- 706
Cdd:PRK08751  209 DIAFLQYTGGTTGVAKGAMLTHR---NLVANMQQAHQwLAGTGKLEEGCEVV--ITALPLYHIFALTAnGLVFMKIggcn 283

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  707 ---DTARNPAALIDLAGRHRVTLAQATPTLWQALvpeLSGPALAGIR------VLVGGEALPAELARRLGD-AGAEVTNM 776
Cdd:PRK08751  284 hliSNPRDMPGFVKELKKTRFTAFTGVNTLFNGL---LNTPGFDQIDfsslkmTLGGGMAVQRSVAERWKQvTGLTLVEA 360

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  777 YGPTETTIWSTSGPTSEDSiRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFg 856
Cdd:PRK08751  361 YGLTETSPAACINPLTLKE-YNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGW- 438

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  857 ppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAYVIPDGP 935
Cdd:PRK08751  439 ------LHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVgVPDEKSGEIVKVVIVKKDPA 512

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 653678460  936 VAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK08751  513 LTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
 1529-2028 3.72e-17

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 87.51  E-value: 3.72e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1529 DDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALhtgalGAPPVGPETPVLLLLDRSPELVVAMLAVLK 1608
Cdd:PRK06155   19 SERTLPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHAL-----AAAGVKRGDRVALMCGNRIEFLDVFLGCAW 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1609 AGGYFIPVDPGYPMARLTRIADTVTPALVLT--------RTGQGGCLPGAEVF--GDVPVVALDRvADRLTAMP--DQR- 1675
Cdd:PRK06155   94 LGAIAVPINTALRGPQLEHILRNSGARLLVVeaallaalEAADPGDLPLPAVWllDAPASVSVPA-GWSTAPLPplDAPa 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1676 PEVTVDPRGLAYSIFTSGSTGQPKGVAVEHigiVRYLSWAAASYPTAG-RRGTLAHSSVGFDLT--MSALFEPLVSGrgv 1752
Cdd:PRK06155  173 PAAAVQPGDTAAILYTSGTTGPSKGVCCPH---AQFYWWGRNSAEDLEiGADDVLYTTLPLFHTnaLNAFFQALLAG--- 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1753 tlmpadATLAdLAEELSGPLAYDYIRLTPSHLRHLVGHWT----GQELPPAARGWVVG---GETLDPALVKQLLElRPDA 1825
Cdd:PRK06155  247 ------ATYV-LEPRFSASGFWPAVRRHGATVTYLLGAMVsillSQPARESDRAHRVRvalGPGVPAALHAAFRE-RFGV 318

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1826 EVINHYGPTET--VIGrVVHPVREAGELavdsplplGRPLGETRLQVLDAWLEAVPVGAVGELFIGGD---GIARGYLGR 1900
Cdd:PRK06155  319 DLLDGYGSTETnfVIA-VTHGSQRPGSM--------GRLAPGFEARVVDEHDQELPDGEPGELLLRADepfAFATGYFGM 389

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1901 PALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLv 1980
Cdd:PRK06155  390 PEKTVEAW--------RNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSEL- 460

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1981 gwfipAEDHPPVTV----------SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PRK06155  461 -----GEDEVMAAVvlrdgtalepVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKV 513
AcpA COG3433
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ...
 1852-2127 4.55e-17

Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442659 [Multi-domain]  Cd Length: 295  Bit Score: 84.42  E-value: 4.55e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1852 AVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMYRTGDLVRWRG 1931
Cdd:COG3433    15 EPPPVIPPAIVQARALLLIVDLQGYFGGFGGEGGLLGAGLLLRIRLLAAAARAPFIPVPYPAQPGRQADDLRLLLRRGLG 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1932 DGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVP 2011
Cdd:COG3433    95 PGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAALRGAGVGLLLIVGAVAALDGLAAAAALAALDKVPPDVVA 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2012 nqWVALDAFPLTPHGKVNHRALPGPTSGRPELEDRYQAPRTPGE-----QLLAELWSTVLGT--DRIGIEDNFFDLGGTS 2084
Cdd:COG3433   175 --ASAVVALDALLLLALKVVARAAPALAAAEALLAAASPAPALEtalteEELRADVAELLGVdpEEIDPDDNLFDLGLDS 252

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|...
gi 653678460 2085 ISVIQLSGACRRAGVEISPKDVFEQPTVRGQAMRAIARSRDDA 2127
Cdd:COG3433   253 IRLMQLVERWRKAGLDVSFADLAEHPTLAAWWALLAAAQAAAA 295
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
 501-977 4.66e-17

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 86.66  E-value: 4.66e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  501 CRRTPGAVAVIegdTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIK---AGAAYLPIDPDFPV 577
Cdd:cd05929     5 DLDRAQVFHQR---RLLLLDVYSIALNRNARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKcgaCPAYKSSRAPRAEA 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  578 ERIAYLLSDARPVLVVTDAATADRLGPDditglvvleETDTGGYPATEPPAVPAGHsaYVIYTSGSTGRPKGVvitRAAL 657
Cdd:cd05929    82 CAIIEIKAAALVCGLFTGGGALDGLEDY---------EAAEGGSPETPIEDEAAGW--KMLYSGGTTGRPKGI---KRGL 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  658 -----DNFLAAMAE-RFPLTAEDRVLATTTVSFDIAGLELYLPLRSGAGVVLADadtARNPAALIDLAGRHRVTLAQATP 731
Cdd:cd05929   148 pggppDNDTLMAAAlGFGPGADSVYLSPAPLYHAAPFRWSMTALFMGGTLVLME---KFDPEEFLRLIERYRVTFAQFVP 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 TLWQAL--VPELSGPA--LAGIRVLVGGEA-LPAELARRLGDAGAEVT-NMYGPTE----TTIwstsgpTSEDSIR-RGS 800
Cdd:cd05929   225 TMFVRLlkLPEAVRNAydLSSLKRVIHAAApCPPWVKEQWIDWGGPIIwEYYGGTEgqglTII------NGEEWLThPGS 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  801 IGVPIDNtQVYVLDANLHPAPIGVLGELHIAGeGLARGYWNRPGLTAEKFLPDPFgppgtrmyRT-GDLVRWSAAGDLEY 879
Cdd:cd05929   299 VGRAVLG-KVHILDEDGNEVPPGEIGEVYFAN-GPGFEYTNDPEKTAAARNEGGW--------STlGDVGYLDEDGYLYL 368

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  880 LGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP-----DGPVAPDALRTELSRTLPDYMI 954
Cdd:cd05929   369 TDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPapgadAGTALAEELIAFLRDRLSRYKC 448

                         490       500
                  ....*....|....*....|...
gi 653678460  955 PAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05929   449 PRSIEFVAELPRDDTGKLYRRLL 471
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1687-2031 4.70e-17

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 85.51  E-value: 4.70e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1687 YSIFTSGSTGQPKGV-------------------------------AVEHIGIVRYL---------SWAAASYPTAGrrG 1726
Cdd:cd05924     7 YILYTGGTTGMPKGVmwrqedifrmlmggadfgtgeftpsedahkaAAAAAGTVMFPapplmhgtgSWTAFGGLLGG--Q 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1727 TLAHSSVGFDltMSALFEpLVSGRGVTLMP--ADATLADLAEELSGPLAYDYirltpSHLRHLVGhwtgqelppaargwv 1804
Cdd:cd05924    85 TVVLPDDRFD--PEEVWR-TIEKHKVTSMTivGDAMARPLIDALRDAGPYDL-----SSLFAISS--------------- 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1805 vGGETLDPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVREAGELAVdsplplgRPLGETRLQVLDAWLEAVPVGAVG 1884
Cdd:cd05924   142 -GGALLSPEVKQGLLELVPNITLVDAFGSSETGFTGSGHSAGSGPETGP-------FTRANPDTVVLDDDGRVVPPGSGG 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1885 ELFIGGDG-IARGYLGRPALTAEKFlpdPAGEpGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAE 1963
Cdd:cd05924   214 VGWIARRGhIPLGYYGDEAKTAETF---PEVD-GVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKS 289

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1964 HPGVEQAVVL-VRDQ---QLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHR 2031
Cdd:cd05924   290 HPAVYDVLVVgRPDErwgQEVVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKADYR 361
PRK08308 PRK08308
acyl-CoA synthetase; Validated
 513-979 5.37e-17

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 85.86  E-value: 5.37e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  513 GDTTLSYRELDERANRLARLLMERGAGAETFvAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLV 592
Cdd:PRK08308    5 NDEEYSKSDFDLRLQRYEEMEQFQEAAGNRF-AVCLKDPFDIITLVFFLKEKGASVLPIHPDTPKEAAIRMAKRAGCHGL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  593 VTDaatadrlgpddiTGLVVLEETDTGgypATEPPAVpaghsayVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTA 672
Cdd:PRK08308   84 LYG------------ESDFTKLEAVNY---LAEEPSL-------LQYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQ 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  673 EDR--VLATTTVSFD-IAGLelYLPLRSGAGVVLAdadTARNPAALIDLAGRHRVTLAQATPTLWQALVPELsgPALAGI 749
Cdd:PRK08308  142 DETpiVACPVTHSYGlICGV--LAALTRGSKPVII---TNKNPKFALNILRNTPQHILYAVPLMLHILGRLL--PGTFQF 214

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 -RVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTSEdsirRGSIGVPIDNTQVYvldanlhpapigvlgel 828
Cdd:PRK08308  215 hAVMTSGTPLPEAWFYKLRERTTYMMQQYGCSEAGCVSICPDMKS----HLDLGNPLPHVSVS----------------- 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  829 hiAGEGLargywNRPGLTAEKFlpdpfgppGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGP 908
Cdd:PRK08308  274 --AGSDE-----NAPEEIVVKM--------GDKEIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPG 338

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460  909 VRRAAAVVREDRPGDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPA 979
Cdd:PRK08308  339 VQEAVVYRGKDPVAGERVKAKVISHEEIDPVQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLLEL 409
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
 517-949 5.71e-17

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 86.75  E-value: 5.71e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL--SDARPVLV-V 593
Cdd:cd05932     7 FTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLehSESKALFVgK 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAATADRLGPDDITGLVVLEETDTG------------GYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:cd05932    87 LDDWKAMAPGVPEGLISISLPPPSAAncqyqwddliaqHPPLEERPTRFPEQLATLIYTSGTTGQPKGVMLTFGSFAWAA 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLAtttvsfdiaglelYLPL--------------RSGAGVVLADA-DTarnpaaLIDLAGRHRVTL 726
Cdd:cd05932   167 QAGIEHIGTEENDRMLS-------------YLPLahvtervfveggslYGGVLVAFAESlDT------FVEDVQRARPTL 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  727 AQATPTLW-------QALVPE--------------------LSGPALAGIRVLVGGEA-LPAELARRLGDAGAEVTNMYG 778
Cdd:cd05932   228 FFSVPRLWtkfqqgvQDKIPQqklnlllkipvvnslvkrkvLKGLGLDQCRLAGCGSApVPPALLEWYRSLGLNILEAYG 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  779 PTETTIWSTSGPTSEDSIrrGSIGVPIDNTQVyvldanlhpaPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgpp 858
Cdd:cd05932   308 MTENFAYSHLNYPGRDKI--GTVGNAGPGVEV----------RISEDGEILVRSPALMMGYYKDPEATAEAFTADGF--- 372

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  859 gtrmYRTGDLVRWSAAGDLEYLGRTDHQVKL-RGFRIELGEIETTLINAGPVrRAAAVVREDRPGDLRLVayVIPDGPV- 936
Cdd:cd05932   373 ----LRTGDKGELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLAEHDRV-EMVCVIGSGLPAPLALV--VLSEEARl 445

                         490
                  ....*....|....
gi 653678460  937 -APDALRTELSRTL 949
Cdd:cd05932   446 rADAFARAELEASL 459
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
 1536-1976 1.21e-16

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 86.01  E-value: 1.21e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAQADRTPG-----WTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAG 1610
Cdd:cd05970    22 VVDAMAKEYPDklalvWCDDAGEERIFTFAELADYSDKTANFFK--AMG---IGKGDTVMLTLKRRYEFWYSLLALHKLG 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1611 GYFIPV-------DPGYPMAR-----LTRIADTVTPALVLTRTGQGGCLPG-AEVFGDVPVVALDRVADRLTAMPD-QRP 1676
Cdd:cd05970    97 AIAIPAthqltakDIVYRIESadikmIVAIAEDNIPEEIEKAAPECPSKPKlVWVGDPVPEGWIDFRKLIKNASPDfERP 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1677 EVTVDPRG----LAYsiFTSGSTGQPKGVAVEHIGIVRYLswAAASY----PTAGRRGTLAHSSVG-------------- 1734
Cdd:cd05970   177 TANSYPCGedilLVY--FSSGTTGMPKMVEHDFTYPLGHI--VTAKYwqnvREGGLHLTVADTGWGkavwgkiygqwiag 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1735 -----FDLTMsalFEP-----LVSGRGVTLMPADATLAD--LAEELSgplAYDYirltpSHLRHLVghwtgqelppaarg 1802
Cdd:cd05970   253 aavfvYDYDK---FDPkalleKLSKYGVTTFCAPPTIYRflIREDLS---RYDL-----SSLRYCT-------------- 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1803 wvVGGETLDPALVKQLLELrPDAEVINHYGPTETVIGRVVHPVREAgelavdSPLPLGRPLGETRLQVLDAWLEAVPVGA 1882
Cdd:cd05970   308 --TAGEALNPEVFNTFKEK-TGIKLMEGFGQTETTLTIATFPWMEP------KPGSMGKPAPGYEIDLIDREGRSCEAGE 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1883 VGELFIGGD-----GIARGYLGRPALTAEKFLPDpagepgarMYRTGDlVRWRG-DGLLDFVGRVDDQVKLRGYRIELGE 1956
Cdd:cd05970   379 EGEIVIRTSkgkpvGLFGGYYKDAEKTAEVWHDG--------YYHTGD-AAWMDeDGYLWFVGRTDDLIKSSGYRIGPFE 449

                         490       500
                  ....*....|....*....|.
gi 653678460 1957 IEARMAEHPGV-EQAVVLVRD 1976
Cdd:cd05970   450 VESALIQHPAVlECAVTGVPD 470
PRK08315 PRK08315
AMP-binding domain protein; Validated
 483-971 1.67e-16

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 85.63  E-value: 1.67e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  483 DTARPLPAASLRERFQQWCRRTPG--AVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLA 560
Cdd:PRK08315    8 PTDVPLLEQTIGQLLDRTAARYPDreALVYRDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  561 VIKAGAAYLPIDPDFPVERIAYLL--SDARpVLVVTDA-------ATADRLGPD---------------DITGLVVLEET 616
Cdd:PRK08315   88 TAKIGAILVTINPAYRLSELEYALnqSGCK-ALIAADGfkdsdyvAMLYELAPElatcepgqlqsarlpELRRVIFLGDE 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  617 DTGG-------------YPATEPPAVPAGHSAY-VI---YTSGSTGRPKGVVIT-RAALDN-FLAAMAERFplTAEDR-- 675
Cdd:PRK08315  167 KHPGmlnfdellalgraVDDAELAARQATLDPDdPIniqYTSGTTGFPKGATLThRNILNNgYFIGEAMKL--TEEDRlc 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  676 --------------VLATTTVsfdiaglelylplrsGAGVVL-ADadtARNPAALIDLAGRHRVTLAQATPTLWQAlvpE 740
Cdd:PRK08315  245 ipvplyhcfgmvlgNLACVTH---------------GATMVYpGE---GFDPLATLAAVEEERCTALYGVPTMFIA---E 303

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  741 LSGPALA---------GIrvlVGGEALPAELARRLGDA--GAEVTNMYGPTETTIWST-SGPTseDSIRR--GSIGVPID 806
Cdd:PRK08315  304 LDHPDFArfdlsslrtGI---MAGSPCPIEVMKRVIDKmhMSEVTIAYGMTETSPVSTqTRTD--DPLEKrvTTVGRALP 378

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  807 NTQVYVLDANLH-PAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrMyRTGDLVRWSAAGDLEYLGRtdh 885
Cdd:PRK08315  379 HLEVKIVDPETGeTVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGW------M-HTGDLAVMDEEGYVNIVGR--- 448

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  886 qVK---LRGfrielG------EIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVI--PDGPVAPDALRTELSRTLPDYM 953
Cdd:PRK08315  449 -IKdmiIRG-----GeniyprEIEEFLYTHPKIQDVQVVgVPDEKYGE-EVCAWIIlrPGATLTEEDVRDFCRGKIAHYK 521

                         570
                  ....*....|....*...
gi 653678460  954 IPAVIVPVPDFPTTPNGK 971
Cdd:PRK08315  522 IPRYIRFVDEFPMTVTGK 539
PRK09274 PRK09274
peptide synthase; Provisional
 1536-1974 1.73e-16

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 85.34  E-value: 1.73e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1536 LVEAqADRTPGWTALRTGD----------RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLA 1605
Cdd:PRK09274   12 LPRA-AQERPDQLAVAVPGgrgadgklayDELSFAELDARSDAIAHGLN--AAG---IGRGMRAVLMVTPSLEFFALTFA 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1606 VLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTrtgqggcLPGAEV--------FGDV-PVVALDR-------VADRLT 1669
Cdd:PRK09274   86 LFKAGAVPVLVDPGMGIKNLKQCLAEAQPDAFIG-------IPKAHLarrlfgwgKPSVrRLVTVGGrllwggtTLATLL 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1670 AMPDQR--PEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTagRRGtlahssvGFDLTM---SALFE 1744
Cdd:PRK09274  159 RDGAAApfPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGI--EPG-------EIDLPTfplFALFG 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1745 PLVSGRGVtLMPADAT---LADLAEELSGPLAYDYIRL--TPSHLRHLVGHWTGQELP-PAARGWVVGGETLDPALVKQL 1818
Cdd:PRK09274  230 PALGMTSV-IPDMDPTrpaTVDPAKLFAAIERYGVTNLfgSPALLERLGRYGEANGIKlPSLRRVISAGAPVPIAVIERF 308

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1819 LE-LRPDAEVINHYGPTE----TVIG-------RVVHPVREAGELavdsplpLGRPLGETRLQV-------LDAWLEA-- 1877
Cdd:PRK09274  309 RAmLPPDAEILTPYGATEalpiSSIEsreilfaTRAATDNGAGIC-------VGRPVDGVEVRIiaisdapIPEWDDAlr 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1878 VPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEPGARMyrtGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEI 1957
Cdd:PRK09274  382 LATGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGQGDVWHRM---GDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPC 458

                         490
                  ....*....|....*...
gi 653678460 1958 EARMAEHPGVEQ-AVVLV 1974
Cdd:PRK09274  459 ERIFNTHPGVKRsALVGV 476
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
1537-2033 1.79e-16

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 85.30  E-value: 1.79e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1537 VEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV 1616
Cdd:PRK06839    8 IEKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIY-ELN---VKKGERIAILSQNSLEYIVLLFAIAKVECIAVPL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1617 DpgypmARLT------RIADTVTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLtampDQRPEVTVDPRGLAYSI- 1689
Cdd:PRK06839   84 N-----IRLTenelifQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVISITSLKEIE----DRKIDNFVEKNESASFIi 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 -FTSGSTGQPKGVAVEHIGIVrylsWAAAsyptagrrgtlaHSSVGFDLTM---SALFEPLVSGRGVTLMPADATLADLA 1765
Cdd:PRK06839  155 cYTSGTTGKPKGAVLTQENMF----WNAL------------NNTFAIDLTMhdrSIVLLPLFHIGGIGLFAFPTLFAGGV 218

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1766 EELSGPLAYDY-IRLTPSHLRHLVghwtgqelppaargwvVGGETLDPALVKQLLELRPD---------------AEVIN 1829
Cdd:PRK06839  219 IIVPRKFEPTKaLSMIEKHKVTVV----------------MGVPTIHQALINCSKFETTNlqsvrwfynggapcpEELMR 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1830 H-----------YGPTETviGRVVHPVREagELAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYL 1898
Cdd:PRK06839  283 EfidrgflfgqgFGMTET--SPTVFMLSE--EDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYW 358

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1899 GRPALTAEkflpdpAGEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRdqQ 1978
Cdd:PRK06839  359 NRPDATEE------TIQDG--WLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGR--Q 428

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1979 LVGW------FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK06839  429 HVKWgeipiaFIVKKSSSVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
 1557-1973 2.17e-16

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 84.16  E-value: 2.17e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALHTGAlgappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVdpgypmarltriADTVTPAL 1636
Cdd:cd05974     1 VSFAEMSARSSRVANFLRSIG-----VGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPA------------TTLLTPDD 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1637 VLTRTGQGGclpgaevfgdvpvvALDRVADRLTAMPDQRpevtvdprgLAYsiFTSGSTGQPKgvAVEHIGIVRYLSWAA 1716
Cdd:cd05974    64 LRDRVDRGG--------------AVYAAVDENTHADDPM---------LLY--FTSGTTSKPK--LVEHTHRSYPVGHLS 116

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1717 ASYPTAGRRGT--LAHSSVGF-DLTMSALFEPLVSGRGVTLMPADATLADLAEELSGPLAYDYIRLTPSHLRHLVGH-WT 1792
Cdd:cd05974   117 TMYWIGLKPGDvhWNISSPGWaKHAWSCFFAPWNAGATVFLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQQdLA 196

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1793 GQELPPaaRGWVVGGETLDPALVKQLLELRpDAEVINHYGPTETV--IGRVVHPVREAGELavdsplplGRPLGETRLQV 1870
Cdd:cd05974   197 SFDVKL--REVVGAGEPLNPEVIEQVRRAW-GLTIRDGYGQTETTalVGNSPGQPVKAGSM--------GRPLPGYRVAL 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1871 LDAWLEAVPVGAVGeLFIGGD---GIARGYLGRPALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLDFVGRVDDQVKL 1947
Cdd:cd05974   266 LDPDGAPATEGEVA-LDLGDTrpvGLMKGYAGDPDKTAHAM--------RGGYYRTGDIAMRDEDGYLTYVGRADDVFKS 336

                         410       420
                  ....*....|....*....|....*.
gi 653678460 1948 RGYRIELGEIEARMAEHPGVEQAVVL 1973
Cdd:cd05974   337 SDYRISPFELESVLIEHPAVAEAAVV 362
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
 637-972 3.13e-16

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 82.55  E-value: 3.13e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  637 VIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVsFDIAGLE--LYLPLRSGAGVVladADTARNPAA 714
Cdd:cd17638     5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPF-FHTFGYKagIVACLLTGATVV---PVAVFDVDA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  715 LIDLAGRHRVTLAQATPTLWQAL--VPELSGPALAGIRVLVGGEA-LPAELARRL-GDAGAE-VTNMYGPTETTIWSTSG 789
Cdd:cd17638    81 ILEAIERERITVLPGPPTLFQSLldHPGRKKFDLSSLRAAVTGAAtVPVELVRRMrSELGFEtVLTAYGLTEAGVATMCR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSEDSIRRGSIGVPIDNTQVYVLDAnlhpapigvlGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLV 869
Cdd:cd17638   161 PGDDAETVATTCGRACPGFEVRIADD----------GEVLVRGYNVMQGYLDDPEATAEAIDADGW-------LHTGDVG 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  870 RWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRlVAYVI--PDGPVAPDALRTELS 946
Cdd:cd17638   224 ELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIgVPDERMGEVG-KAFVVarPGVTLTEEDVIAWCR 302

                         330       340
                  ....*....|....*....|....*.
gi 653678460  947 RTLPDYMIPAVIVPVPDFPTTPNGKL 972
Cdd:cd17638   303 ERLANYKVPRFVRFLDELPRNASGKV 328
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
 514-904 3.13e-16

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 84.95  E-value: 3.13e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  514 DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVV 593
Cdd:PRK04319   71 KEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLI 150

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAATADRLGPDDITGL--VVL---EETDTGGY-----------PATEPPAVPAGHSAYVIYTSGSTGRPKGVV------ 651
Cdd:PRK04319  151 TTPALLERKPADDLPSLkhVLLvgeDVEEGPGTldfnalmeqasDEFDIEWTDREDGAILHYTSGSTGKPKGVLhvhnam 230

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  652 ----IT-RAALDnflaamaerfpLTAEDRVLAT------TTVSFDIAGlelylPLRSGAGVVLADADTarNPAALIDLAG 720
Cdd:PRK04319  231 lqhyQTgKYVLD-----------LHEDDVYWCTadpgwvTGTSYGIFA-----PWLNGATNVIDGGRF--SPERWYRILE 292

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQATPTLWQALV---PEL-SGPALAGIR-VLVGGEALPAELARrlgdAGAEVTNMygPTETTIWST-SG----- 789
Cdd:PRK04319  293 DYKVTVWYTAPTAIRMLMgagDDLvKKYDLSSLRhILSVGEPLNPEVVR----WGMKVFGL--PIHDNWWMTeTGgimia 366

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 --PTSEdsIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAgEG---LARGYWNRPGLTAEKFLPDpfgppgtrMYR 864
Cdd:PRK04319  367 nyPAMD--IKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIK-KGwpsMMRGIWNNPEKYESYFAGD--------WYV 435

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|
gi 653678460  865 TGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLI 904
Cdd:PRK04319  436 SGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLM 475
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1555-1998 3.42e-16

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 84.05  E-value: 3.42e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1555 RSLTFAGLDAQANRLAHALHtgalgAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTP 1634
Cdd:cd05910     1 SRLSFRELDERSDRIAQGLT-----AYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEP 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1635 alvltrtgqggclpgaEVFGDVPVvaldrvADRLTAMpdqrpevtvdprglaysIFTSGSTGQPKGVAVEHIGIVRYLSW 1714
Cdd:cd05910    76 ----------------DAFIGIPK------ADEPAAI-----------------LFTSGSTGTPKGVVYRHGTFAAQIDA 116

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1715 AAASYPTAGRRGTLAhssvGFDLTmsALFEPLVsgrGVT--------LMPADATLADLAEELSgPLAYDYIRLTPSHLRH 1786
Cdd:cd05910   117 LRQLYGIRPGEVDLA----TFPLF--ALFGPAL---GLTsvipdmdpTRPARADPQKLVGAIR-QYGVSIVFGSPALLER 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1787 LVGHWTGQELP-PAARGWVVGGETLDPALVKQLLE-LRPDAEVINHYGPTETVigrvvhPVREAGE---LAVDSPLP--- 1858
Cdd:cd05910   187 VARYCAQHGITlPSLRRVLSAGAPVPIALAARLRKmLSDEAEILTPYGATEAL------PVSSIGSrelLATTTAATsgg 260

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 ----LGRPLGETRLQVLDA-------WLEA--VPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPAGEpgaRMYRTGD 1925
Cdd:cd05910   261 agtcVGRPIPGVRVRIIEIddepiaeWDDTleLPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDNSEG---FWHRMGD 337

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 653678460 1926 LVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGveqavvlVRDQQLVGWFIPAEDHPPVTVSALR 1998
Cdd:cd05910   338 LGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPG-------VRRSALVGVGKPGCQLPVLCVEPLP 403
PRK08315 PRK08315
AMP-binding domain protein; Validated
 1530-2028 4.42e-16

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 84.09  E-value: 4.42e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPGWTAL--RTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVL 1607
Cdd:PRK08315   15 EQTIGQLLDRTAARYPDREALvyRDQGLRWTYREFNEEVDALAKGLL--ALG---IEKGDRVGIWAPNVPEWVLTQFATA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1608 KAGGYFIPVDPGYPMARLTRIADTV-TPALVLTR-------------------TGQGGCLPGAEV--------FGDVPVV 1659
Cdd:PRK08315   90 KIGAILVTINPAYRLSELEYALNQSgCKALIAADgfkdsdyvamlyelapelaTCEPGQLQSARLpelrrvifLGDEKHP 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1660 ALDRVADrLTAMPDQRPEVTVDPRGLAYSI-------FTSGSTGQPKGVAVEHIGIVR--YLSWAAASYpTAGRR----- 1725
Cdd:PRK08315  170 GMLNFDE-LLALGRAVDDAELAARQATLDPddpiniqYTSGTTGFPKGATLTHRNILNngYFIGEAMKL-TEEDRlcipv 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1726 ----------GTLAHSSVGfdLTM---SALFEPLvsgrgvtlmpadATLADLAEE---------------LSGPL--AYD 1775
Cdd:PRK08315  248 plyhcfgmvlGNLACVTHG--ATMvypGEGFDPL------------ATLAAVEEErctalygvptmfiaeLDHPDfaRFD 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1776 YirltpSHLRhlvghwTGqelppaargwVVGGETLDPALVKQLLELRPDAEVINHYGPTETvigrvvHPVreAGELAVDS 1855
Cdd:PRK08315  314 L-----SSLR------TG----------IMAGSPCPIEVMKRVIDKMHMSEVTIAYGMTET------SPV--STQTRTDD 364

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1856 PLPL-----GRPLGETRLQVLDAWL-EAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRW 1929
Cdd:PRK08315  365 PLEKrvttvGRALPHLEVKIVDPETgETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAI--DADG-----WMHTGDLAVM 437

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1930 RGDGLLDFVGRVDDQVkLRG----Y-RielgEIEARMAEHPGVEQA-VVLVRDQ----QLVGWFIPAEDHPpVTVSALRR 1999
Cdd:PRK08315  438 DEEGYVNIVGRIKDMI-IRGgeniYpR----EIEEFLYTHPKIQDVqVVGVPDEkygeEVCAWIILRPGAT-LTEEDVRD 511

                         570       580
                  ....*....|....*....|....*....
gi 653678460 2000 FCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PRK08315  512 FCRGKIAHYKIPRYIRFVDEFPMTVTGKI 540
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
 499-950 5.38e-16

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 84.02  E-value: 5.38e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  499 QWCRRTPGAVAVIEGD-----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDP 573
Cdd:cd05921     3 HWARQAPDRTWLAEREgnggwRRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  574 DFPV-----ERIAYLLSDARPVLVVTDAATA-----DRLGPDDITGLVV---LEETDTGGYP---ATEP-PAVPAGHS-- 634
Cdd:cd05921    83 AYSLmsqdlAKLKHLFELLKPGLVFAQDAAPfaralAAIFPLGTPLVVSrnaVAGRGAISFAelaATPPtAAVDAAFAav 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 -----AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDrvlatTTVSFDIAGLELYLPLRSGAGVVLADADTa 709
Cdd:cd05921   163 gpdtvAKFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEE-----PPVLVDWLPWNHTFGGNHNFNLVLYNGGT- 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  710 rnpaALID----LAGRHRVTLA---QATPTL-------WQALVPELSG-PAL-----AGIRVLV-GGEALPAELARRLGD 768
Cdd:cd05921   237 ----LYIDdgkpMPGGFEETLRnlrEISPTVyfnvpagWEMLVAALEKdEALrrrffKRLKLMFyAGAGLSQDVWDRLQA 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  769 -AGAEV------TNMYGPTET--TIWSTSGPTSedsiRRGSIGVPIDNTQVYVldanlhpAPIGVLGELHIAGEGLARGY 839
Cdd:cd05921   313 lAVATVgeripmMAGLGATETapTATFTHWPTE----RSGLIGLPAPGTELKL-------VPSGGKYEVRVKGPNVTPGY 381

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  840 WNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGD----LEYLGRTDHQVKLR-GFRIELGEIETTLINA-GPVRRAA 913
Cdd:cd05921   382 WRQPELTAQAFDEEGF-------YCLGDAAKLADPDDpakgLVFDGRVAEDFKLAsGTWVSVGPLRARAVAAcAPLVHDA 454

                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 653678460  914 AVVREDRPGdlrLVAYVIPDgpvaPDALRtELSRTLP 950
Cdd:cd05921   455 VVAGEDRAE---VGALVFPD----LLACR-RLVGLQE 483
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
501-977 6.02e-16

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 84.63  E-value: 6.02e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  501 CRRTPGAVAVIEGDTT-LSYRELDERANRLARLLmERGAGAETFVAVLLPRSADLLVTLLAVIKAGaaYLPIDPDFPVEr 579
Cdd:PRK06814  642 KIHGFKKLAVEDPVNGpLTYRKLLTGAFVLGRKL-KKNTPPGENVGVMLPNANGAAVTFFALQSAG--RVPAMINFSAG- 717

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  580 IAYLLSDARPV---LVVTDAA--TADRLGPDdITGL------VVLEETD-------------TGGYPATEPPAVPAGHSA 635
Cdd:PRK06814  718 IANILSACKAAqvkTVLTSRAfiEKARLGPL-IEALefgiriIYLEDVRaqigladkikgllAGRFPLVYFCNRDPDDPA 796

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 YVIYTSGSTGRPKGVVITRAaldNFLAAMAE---RFPLTAEDRVLATTTV--SFDIAGlELYLPLRSGAGVVLAdadtar 710
Cdd:PRK06814  797 VILFTSGSEGTPKGVVLSHR---NLLANRAQvaaRIDFSPEDKVFNALPVfhSFGLTG-GLVLPLLSGVKVFLY------ 866

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  711 nPAALidlagRHRV----------TLAQATPTLwqalvpeLSGPA-------LAGIR-VLVGGEALPAElARRL--GDAG 770
Cdd:PRK06814  867 -PSPL-----HYRIipeliydtnaTILFGTDTF-------LNGYAryahpydFRSLRyVFAGAEKVKEE-TRQTwmEKFG 932

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  771 AEVTNMYGPTETT-IWSTSGPTSEdsiRRGSIG--VPIdntqvyvLDANLHPAP-IGVLGELHIAGEGLARGYwnrpgLT 846
Cdd:PRK06814  933 IRILEGYGVTETApVIALNTPMHN---KAGTVGrlLPG-------IEYRLEPVPgIDEGGRLFVRGPNVMLGY-----LR 997

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  847 AEKflPDPFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVRED-RPGDlR 925
Cdd:PRK06814  998 AEN--PGVLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDaRKGE-R 1074

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460  926 LVAYVipdgpVAPDALRTEL-----SRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06814 1075 IILLT-----TASDATRAAFlahakAAGASELMVPAEIITIDEIPLLGTGKIDYVAV 1126
PRK05850 PRK05850
acyl-CoA synthetase; Validated
 516-976 1.21e-15

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 83.07  E-value: 1.21e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETfVAVLLPRSADLLVTLLAVIKAG--AAYLPIdPDFPV--ERIAYLLSDARPVL 591
Cdd:PRK05850   35 TLTWSQLYRRTLNVAEELRRHGSTGDR-AVILAPQGLEYIVAFLGALQAGliAVPLSV-PQGGAhdERVSAVLRDTSPSV 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  592 VVTDAATADRLG------PDDITGLVVleETDTGGYPA-TEPPAVPAGHS--AYVIYTSGSTGRPKGVVIT-RAALDNFL 661
Cdd:PRK05850  113 VLTTSAVVDDVTeyvapqPGQSAPPVI--EVDLLDLDSpRGSDARPRDLPstAYLQYTSGSTRTPAGVMVShRNVIANFE 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAERFPLTAEDRVLATTTVSFdiagLELY----------LPLRSGAGVVLADADT------------ARNPAAL---- 715
Cdd:PRK05850  191 QLMSDYFGDTGGVPPPDTTVVSW----LPFYhdmglvlgvcAPILGGCPAVLTSPVAflqrparwmqllASNPHAFsaap 266

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  716 ---IDLAGRhRVTLAqatptlwqalvpELSGPALAGIRVLV-GGEAL-PAELAR------RLGDAGAEVTNMYGPTETTI 784
Cdd:PRK05850  267 nfaFELAVR-KTSDD------------DMAGLDLGGVLGIIsGSERVhPATLKRfadrfaPFNLRETAIRPSYGLAEATV 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  785 WSTSGPTSE---------DSIRRG--------------SIGVPiDNTQVYVLDANLH-PAPIGVLGELHIAGEGLARGYW 840
Cdd:PRK05850  334 YVATREPGQppesvrfdyEKLSAGhakrcetgggtplvSYGSP-RSPTVRIVDPDTCiECPAGTVGEIWVHGDNVAAGYW 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  841 NRPGLTAEKF---LPDPfgPPGTRM---YRTGDLVRWSaAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAA 914
Cdd:PRK05850  413 QKPEETERTFgatLVDP--SPGTPEgpwLRTGDLGFIS-EGELFIVGRIKDLLIVDGRNHYPDDIEATIQEITGGRVAAI 489

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460  915 VVREDRPGDLRLVAYVIPDGPVAPDALR----------TELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:PRK05850  490 SVPDDGTEKLVAIIELKKRGDSDEEAMDrlrtvkrevtSAISKSHGLSVADLVLVAPGSIPITTSGKIRRAA 561
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
 1681-1984 1.90e-15

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 81.64  E-value: 1.90e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1681 DPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTagrrgtlahsSVGfDLTMSAL---------FEPLVSGRG 1751
Cdd:cd17640    86 DSDDLATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPP----------QPG-DRFLSILpiwhsyersAEYFIFACG 154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1752 VTLMPAD--ATLADLAEE-----LSGPLAYDYIRL-------TPSHLRHLVGHW--TGQELPPAargwVVGGETLDPALV 1815
Cdd:cd17640   155 CSQAYTSirTLKDDLKRVkphyiVSVPRLWESLYSgiqkqvsKSSPIKQFLFLFflSGGIFKFG----ISGGGALPPHVD 230

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1816 KQLLELrpDAEVINHYGPTET----VIGRVVHPVREAgelavdsplpLGRPLGETRLQVLDAWLEAV-PVGAVGELFIGG 1890
Cdd:cd17640   231 TFFEAI--GIEVLNGYGLTETspvvSARRLKCNVRGS----------VGRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRG 298

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1891 DGIARGYLGRPALTAEKFLPDPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLR-GYRIELGEIEARMAEHPGVEQ 1969
Cdd:cd17640   299 PQVMKGYYKNPEATSKVLDSDG-------WFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQ 371

                         330
                  ....*....|....*
gi 653678460 1970 AVVLVRDQQLVGWFI 1984
Cdd:cd17640   372 IMVVGQDQKRLGALI 386
PRK07470 PRK07470
acyl-CoA synthetase; Validated
 1541-2046 2.24e-15

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 82.01  E-value: 2.24e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD--- 1617
Cdd:PRK07470   17 ARRFPDRIALVWGDRSWTWREIDARVDALAAALA--ARG---VRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNfrq 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 -PGyPMARLTR-------IADTVTP----ALVLTRTGQGGCLPGAEVFGDVPVVALdrVADRLTAMPdqrPEVTVDPRGL 1685
Cdd:PRK07470   92 tPD-EVAYLAEasgaramICHADFPehaaAVRAASPDLTHVVAIGGARAGLDYEAL--VARHLGARV---ANAAVDHDDP 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1686 AYSIFTSGSTGQPKGvAVehigivrylswaaasyptagrrgtLAHSSVGF-------DLT-------MSALFEPLVSGRG 1751
Cdd:PRK07470  166 CWFFFTSGTTGRPKA-AV------------------------LTHGQMAFvitnhlaDLMpgtteqdASLVVAPLSHGAG 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1752 VTLM----PADATLADLAEELSGPLAYDYI---RLT-----PSHLRHLVGHwtgqelpPAA--------RGWVVGGETLD 1811
Cdd:PRK07470  221 IHQLcqvaRGAATVLLPSERFDPAEVWALVerhRVTnlftvPTILKMLVEH-------PAVdrydhsslRYVIYAGAPMY 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1812 PAlvKQLLELRPDAEVI-NHYGPTE-----TVIGRVVHPVREAGELAVDSplpLGRPLGETRLQVLDAWLEAVPVGAVGE 1885
Cdd:PRK07470  294 RA--DQKRALAKLGKVLvQYFGLGEvtgniTVLPPALHDAEDGPDARIGT---CGFERTGMEVQIQDDEGRELPPGETGE 368

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1886 LFIGGDGIARGYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHP 1965
Cdd:PRK07470  369 ICVIGPAVFAGYYNNPEANAKAFRDG--------WFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHP 440

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1966 GV-EQAVVLVRDQQL--VGW-FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALpgptsgRP 2041
Cdd:PRK07470  441 AVsEVAVLGVPDPVWgeVGVaVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMV------RE 514


                  ....*
gi 653678460 2042 ELEDR 2046
Cdd:PRK07470  515 ELEER 519
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
 1534-2035 2.53e-15

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 81.58  E-value: 2.53e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1534 HQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYF 1613
Cdd:PRK13383   38 YTLLAVTAARWPGRTAIIDDDGALSYRELQRATESLA-----RRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADV 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 IPVDPGYPmarltriADTVTPALvltRTGQGGCLPGAEVFGDVPVVALDRVA--DRLTAMPDQ---RPEVTVDPRGLays 1688
Cdd:PRK13383  113 VPISTEFR-------SDALAAAL---RAHHISTVVADNEFAERIAGADDAVAviDPATAGAEEsggRPAVAAPGRIV--- 179

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1689 IFTSGSTGQPKGVAVE---HIGIVRYLSWAAASYPTAGRRGTLA---HSSVGFDLTMsalfepLVSGRGVTLMP-----A 1757
Cdd:PRK13383  180 LLTSGTTGKPKGVPRApqlRSAVGVWVTILDRTRLRTGSRISVAmpmFHGLGLGMLM------LTIALGGTVLThrhfdA 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1758 DATLADL----AEELSG-PLAYDYIRLTPSHLRhlvghwtGQELPPAARGWVVGGETLDPALVKQLLELRPDAeVINHYG 1832
Cdd:PRK13383  254 EAALAQAslhrADAFTAvPVVLARILELPPRVR-------ARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDI-LYNGYG 325

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1833 PTETVIGRVVHPvreaGELAvDSPLPLGRPLGETRLQVLDAwlEAVPVG--AVGELFIGGDGIARGYLGrpalTAEKFLP 1910
Cdd:PRK13383  326 STEVGIGALATP----ADLR-DAPETVGKPVAGCPVRILDR--NNRPVGprVTGRIFVGGELAGTRYTD----GGGKAVV 394

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1911 DPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQL---VGWFIPA 1986
Cdd:PRK13383  395 DG-------MTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVaDNAVIGVPDERFghrLAAFVVL 467

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 653678460 1987 EDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPG 2035
Cdd:PRK13383  468 HPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKELPG 516
PRK13382 PRK13382
bile acid CoA ligase;
1539-2036 2.78e-15

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 81.73  E-value: 2.78e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1539 AQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDP 1618
Cdd:PRK13382   51 IAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRV-----VGIMCRNHRGFVEALLAANRIGADILLLNT 125

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1619 GYPMARLtriadtvtpALVLTRTGQGGCLPGAEVFGDVPVVALDRV-ADRLTAMPDQRPEVTVD--------------PR 1683
Cdd:PRK13382  126 SFAGPAL---------AEVVTREGVDTVIYDEEFSATVDRALADCPqATRIVAWTDEDHDLTVEvliaahagqrpeptGR 196

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1684 GLAYSIFTSGSTGQPKGVAVEHIG-------IVRYLSWAA--ASYPTAGRRGTLAHSSVGFDLTMSAlfePLVSGRGVTl 1754
Cdd:PRK13382  197 KGRVILLTSGTTGTPKGARRSGPGgigtlkaILDRTPWRAeePTVIVAPMFHAWGFSQLVLAASLAC---TIVTRRRFD- 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 mpADATLADLAEELSGPLAydyirLTPSHLRHLVghwtgqELPPAARGWVVG---------GETLDPALVKQLLELRPDA 1825
Cdd:PRK13382  273 --PEATLDLIDRHRATGLA-----VVPVMFDRIM------DLPAEVRNRYSGrslrfaaasGSRMRPDVVIAFMDQFGDV 339

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1826 eVINHYGPTEtvigrvvhpvreAGELAVDSPLPL-------GRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYl 1898
Cdd:PRK13382  340 -IYNNYNATE------------AGMIATATPADLraapdtaGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGY- 405

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1899 grpaltaekfLPDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRD- 1976
Cdd:PRK13382  406 ----------TSGSTKDFHDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIgVDDe 475

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 653678460 1977 ---QQLVGWFIPAEDhPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:PRK13382  476 qygQRLAAFVVLKPG-ASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQAR 537
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
 501-974 2.96e-15

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 81.77  E-value: 2.96e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  501 CRRTpgAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERI 580
Cdd:PLN02860   19 LRGN--AVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  581 AYLLSDARPVLVVTDAAT---ADRLGPDDITGL---VVLEETDTGGYPA-----------------TEPPAVPAGHSAYV 637
Cdd:PLN02860   97 KSAMLLVRPVMLVTDETCsswYEELQNDRLPSLmwqVFLESPSSSVFIFlnsflttemlkqralgtTELDYAWAPDDAVL 176

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  638 I-YTSGSTGRPKGVVITRAAL-DNFLAAMAerFPLTAEDRVLATTTVSFDIAGLELYLP-LRSGAGVVLA---DADTArn 711
Cdd:PLN02860  177 IcFTSGTTGRPKGVTISHSALiVQSLAKIA--IVGYGEDDVYLHTAPLCHIGGLSSALAmLMVGACHVLLpkfDAKAA-- 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  712 paalIDLAGRHRVTLAQATPTLWQALVP----ELSGPALAGIR-VLVGGEALPAEL--ARRLGDAGAEVTNMYGPTET-- 782
Cdd:PLN02860  253 ----LQAIKQHNVTSMITVPAMMADLISltrkSMTWKVFPSVRkILNGGGSLSSRLlpDAKKLFPNAKLFSAYGMTEAcs 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  783 --TIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANL--HPAP-----IGVLGELHIaGEGLARG------YWNRPGLTA 847
Cdd:PLN02860  329 slTFMTLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCvgKPAPhvelkIGLDESSRV-GRILTRGphvmlgYWGQNSETA 407

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  848 EKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLrL 926
Cdd:PLN02860  408 SVLSNDGW-------LDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVgVPDSRLTEM-V 479

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  927 VAYV-IPDG---------------PVAPDALRTELS-RTLPDYMIPAVIVPVPD-FPTTPNGKLDR 974
Cdd:PLN02860  480 VACVrLRDGwiwsdnekenakknlTLSSETLRHHCReKNLSRFKIPKLFVQWRKpFPLTTTGKIRR 545
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 636-973 3.21e-15

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 79.73  E-value: 3.21e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 YVIYTSGSTGRPKGVV-----ITRAALDNFLAAMAERFP--LTAEDRVLATTTVSFDIAglelylPLRSGAGVVLADADT 708
Cdd:cd05924     7 YILYTGGTTGMPKGVMwrqedIFRMLMGGADFGTGEFTPseDAHKAAAAAAGTVMFPAP------PLMHGTGSWTAFGGL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  709 AR-----------NPAALIDLAGRHRVTL------AQATPtlwqaLVPELSGPA---LAGIRVLV-GGEALPAELARRLG 767
Cdd:cd05924    81 LGgqtvvlpddrfDPEEVWRTIEKHKVTSmtivgdAMARP-----LIDALRDAGpydLSSLFAISsGGALLSPEVKQGLL 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  768 DA--GAEVTNMYGPTETTIWSTSGPTSEDSIRRGSIGVpidNTQVYVLDANLHPAPIGVLGELHIAGEGL-ARGYWNRPG 844
Cdd:cd05924   156 ELvpNITLVDAFGSSETGFTGSGHSAGSGPETGPFTRA---NPDTVVLDDDGRVVPPGSGGVGWIARRGHiPLGYYGDEA 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  845 LTAEKFlpdpFGPPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDL 924
Cdd:cd05924   233 KTAETF----PEVDGVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQ 308

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460  925 RLVAYVIPDGPVAPD--ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd05924   309 EVVAVVQLREGAGVDleELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
1656-2033 3.96e-15

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 79.70  E-value: 3.96e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1656 VPVVALDRV-ADRLTAmpDQRPEVTVDPrGLAYSIFTSGSTGQPKGVAVEHIGIVrylSWAAASYPTAGRRGTL-----A 1729
Cdd:PRK07824   10 LPVPAQDERrAALLRD--ALRVGEPIDD-DVALVVATSGTTGTPKGAMLTAAALT---ASADATHDRLGGPGQWllalpA 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1730 HSSVGFDLTMSALF---EPLVSGRGVTLMPADatLADLAEELSGPLAYdyIRLTPSHLRHLVGHWTGQELPPAARGWVVG 1806
Cdd:PRK07824   84 HHIAGLQVLVRSVIagsEPVELDVSAGFDPTA--LPRAVAELGGGRRY--TSLVPMQLAKALDDPAATAALAELDAVLVG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1807 GETLDPALVKQLLELRpdAEVINHYGPTETVIGRVVHpvreagelavdsplplGRPLGETRLQVLDawleavpvgavGEL 1886
Cdd:PRK07824  160 GGPAPAPVLDAAAAAG--INVVRTYGMSETSGGCVYD----------------GVPLDGVRVRVED-----------GRI 210

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1887 FIGGDGIARGYLGRPAltaekflPDPAGEPGarMYRTGDLVRWrGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPG 1966
Cdd:PRK07824  211 ALGGPTLAKGYRNPVD-------PDPFAEPG--WFRTDDLGAL-DDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPA 280

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1967 VEQAVVL-VRDQQL---VGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK07824  281 VADCAVFgLPDDRLgqrVVAAVVGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRAL 351
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
 1555-2033 4.55e-15

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 81.21  E-value: 4.55e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1555 RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARL-TRIADtVT 1633
Cdd:cd05967    81 RTYTYAELLDEVSRLAGVLR--KLG---VVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELaSRIDD-AK 154

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1634 PALVLTrtGQGGCLPGAEV------------------------FGDVPVVALDRVAD------RLTAMPdqRPEVTVDPR 1683
Cdd:cd05967   155 PKLIVT--ASCGIEPGKVVpykplldkalelsghkphhvlvlnRPQVPADLTKPGRDldwselLAKAEP--VDCVPVAAT 230

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1684 GLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGTL-AHSSVGFDLTMSAL-FEPLVSGrGVTLM------ 1755
Cdd:cd05967   231 DPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWwAASDVGWVVGHSYIvYGPLLHG-ATTVLyegkpv 309

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1756 --PADATLADLAEE------LSGPLAYDYIRLTPSHLRHLVGHWTGqelppAARGWVVGGETLDPAlVKQLLELRPDAEV 1827
Cdd:cd05967   310 gtPDPGAFWRVIEKyqvnalFTAPTAIRAIRKEDPDGKYIKKYDLS-----SLRTLFLAGERLDPP-TLEWAENTLGVPV 383

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1828 INHYGPTET-------VIGRVVHPVReAGelavdSPlplGRPLGETRLQVLDAWLEAVPVGAVGELFIGGD---GIARGY 1897
Cdd:cd05967   384 IDHWWQTETgwpitanPVGLEPLPIK-AG-----SP---GKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPlppGCLLTL 454

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1898 LGRPALTAEKFLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD 1976
Cdd:cd05967   455 WKNDERFKKLYLSKFPG-----YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVaECAVVGVRD 529

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1977 ----QQLVGWFIPAEDHPPVT---VSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05967   530 elkgQVPLGLVVLKEGVKITAeelEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTL 593
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
 517-921 4.90e-15

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 81.25  E-value: 4.90e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPV-----ERIAYLLSDARPVL 591
Cdd:PRK12582   81 VTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVSPAYSLmshdhAKLKHLFDLVKPRV 160

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  592 V-VTDAATADR----LGPDDITGLVVLEETD-TGGYP----ATEPP--AVPAGHS-------AYVIYTSGSTGRPKGVVI 652
Cdd:PRK12582  161 VfAQSGAPFARalaaLDLLDVTVVHVTGPGEgIASIAfadlAATPPtaAVAAAIAaitpdtvAKYLFTSGSTGMPKAVIN 240

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  653 TRAALDNFLAAMAERFPLTAEDRVlattTVSFD-------IAGLELYLPLRSGAGVVLAD---------ADTARNpaaLI 716
Cdd:PRK12582  241 TQRMMCANIAMQEQLRPREPDPPP----PVSLDwmpwnhtMGGNANFNGLLWGGGTLYIDdgkplpgmfEETIRN---LR 313

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  717 DLAGrhrvTLAQATPTLWQALVPEL-SGPALA-----GIRVL-VGGEALPAELARRLGD-AGAE------VTNMYGPTET 782
Cdd:PRK12582  314 EISP----TVYGNVPAGYAMLAEAMeKDDALRrsffkNLRLMaYGGATLSDDLYERMQAlAVRTtghripFYTGYGATET 389

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  783 TiwSTSGPTSEDSIRRGSIGVPIDNTQVYVldanlhpAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrm 862
Cdd:PRK12582  390 A--PTTTGTHWDTERVGLIGLPLPGVELKL-------APVGDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGF------- 453

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  863 YRTGDLVRWSAAGDLE----YLGRTDHQVKL-RGFRIELGEIETTLINA-GPVRRAAAVVREDRP 921
Cdd:PRK12582  454 YRLGDAARFVDPDDPEkgliFDGRVAEDFKLsTGTWVSVGTLRPDAVAAcSPVIHDAVVAGQDRA 518
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
 1533-2033 8.31e-15

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 80.23  E-value: 8.31e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALR-----TGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVL 1607
Cdd:cd05968    63 VEQLLDKWLADTRTRPALRwegedGTSRTLTYGELLYEVKRLA-----NGLRALGVGKGDRVGIYLPMIPEIVPAFLAVA 137

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1608 KAGGYFIPVDPGY-PMARLTRIADTVTPALV-------------LTRTGQGGCLPGAEV----------FGDVPVVALDR 1663
Cdd:cd05968   138 RIGGIVVPIFSGFgKEAAATRLQDAEAKALItadgftrrgrevnLKEEADKACAQCPTVekvvvvrhlgNDFTPAKGRDL 217

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1664 VADRLTAMPDQRPEVT--VDPRGLaysIFTSGSTGQPKGVAVEHIGIVryLSWAA-ASYPTAGRRGTLAH--SSVGFDLT 1738
Cdd:cd05968   218 SYDEEKETAGDGAERTesEDPLMI---IYTSGTTGKPKGTVHVHAGFP--LKAAQdMYFQFDLKPGDLLTwfTDLGWMMG 292

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1739 MSALFEPLVSGRGVTL------MPADATLADLAEELSgplaYDYIRLTPSHLRHLVGH---WTGQELPPAARGWVVGGET 1809
Cdd:cd05968   293 PWLIFGGLILGATMVLydgapdHPKADRLWRMVEDHE----ITHLGLSPTLIRALKPRgdaPVNAHDLSSLRVLGSTGEP 368

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1810 LDPALVKQLLELRPDAE--VINHYGPTET---VIGRVvhPVREAGELAVDSPLPlgrplgETRLQVLDAwlEAVPV-GAV 1883
Cdd:cd05968   369 WNPEPWNWLFETVGKGRnpIINYSGGTEIsggILGNV--LIKPIKPSSFNGPVP------GMKADVLDE--SGKPArPEV 438

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1884 GELFIGGD--GIARGYLGRPALTAEKFLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARM 1961
Cdd:cd05968   439 GELVLLAPwpGMTRGFWRDEDRYLETYWSRFDN-----VWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVL 513

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 1962 AEHPGVEQAVVL-----VRDQQLVGWFIPAEDHPPVTVSA--LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05968   514 NAHPAVLESAAIgvphpVKGEAIVCFVVLKPGVTPTEALAeeLMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVI 592
E-C_NRPS cd19544
Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); ...
 1084-1406 1.05e-14

Dual Epimerization/Condensation (E/C) domains of nonribosomal peptide synthetases (NRPSs); Dual function Epimerization/Condensation (E/C) domains have both an epimerization and a DCL condensation activity. Dual E/C domains first epimerize the substrate amino acid to produce a D-configuration, then catalyze the condensation between the D-aminoacyl/peptidyl-PCP donor and a L-aminoacyl-PCP acceptor. They are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor ((D)C(L)); this is in contrast with the standard LCL domains which catalyze peptide bond formation between two L-amino acids, and the restriction of ribosomes to use only L-amino acids. These Dual E/C domains contain an extended His-motif (HHx(N)GD) near the N-terminus of the domain in addition to the standard Condensation (C) domain active site motif (HHxxxD). C domains of nonribosomal peptide synthetases (NRPSs) catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains, these include the DCL-type, LCL-type, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C domains, and the X-domain.


Pssm-ID: 380466 [Multi-domain]  Cd Length: 413  Bit Score: 79.02  E-value: 1.05e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1084 PLSPMQESIW---LADQvsAGDsVYNVPLALRLigpLDRGALRR---TLNRVVDRHEMLRTRIHpgPDGM--PVQV---- 1151
Cdd:cd19544     3 PLAPLQEGILfhhLLAE--EGD-PYLLRSLLAF---DSRARLDAflaALQQVIDRHDILRTAIL--WEGLsePVQVvwrq 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1152 ---------ADPAGGGAALTERDAAPGtdlaqlllaeaalGFTLATEHP--LRAVLWRlDER--EHVLLLTAHHVAVDAW 1218
Cdd:cd19544    75 aelpveeltLDPGDDALAQLRARFDPR-------------RYRLDLRQAplLRAHVAE-DPAngRWLLLLLFHHLISDHT 140

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1219 SMDLIQRDLTELYAAdtghREPELDEPAL-----AQAdyavwqRQSAQRGRYAAeldFWRTELtgavatevaGDLPRPAT 1293
Cdd:cd19544   141 SLELLLEEIQAILAG----RAAALPPPVPyrnfvAQA------RLGASQAEHEA---FFREML---------GDVDEPTA 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1294 P---------GGGGGAVEFALAPRQIQGIRELARRCGTTVSTV-------VLSALqtllyriTGGADVVIGSTVSGR--G 1355
Cdd:cd19544   199 PfglldvqgdGSDITEARLALDAELAQRLRAQARRLGVSPASLfhlawalVLARC-------SGRDDVVFGTVLSGRmqG 271

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1356 NPRLDNLVGCLVNTVVLRGDLSGAPTfHELLRRTHARTADALAHqelpfEH 1406
Cdd:cd19544   272 GAGADRALGMFINTLPLRVRLGGRSV-REAVRQTHARLAELLRH-----EH 316
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
 514-903 1.18e-14

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 78.93  E-value: 1.18e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  514 DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVV 593
Cdd:cd05940     1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAatadrlgpdditglvvleetdtggypateppavpaghsAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAE 673
Cdd:cd05940    81 VDA--------------------------------------ALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALPS 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  674 DRvLATTTVSFDIAGLELYLP--LRSGAGVVLADADTARNpaaLIDLAGRHRVTLAQATPTLWQAL--VPELSGPALAGI 749
Cdd:cd05940   123 DV-LYTCLPLYHSTALIVGWSacLASGATLVIRKKFSASN---FWDDIRKYQATIFQYIGELCRYLlnQPPKPTERKHKV 198

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 RVLVGGEALPA---ELARRLGDagAEVTNMYGPTETTI--WSTSGptsedsiRRGSIG--------------VPID-NTQ 809
Cdd:cd05940   199 RMIFGNGLRPDiweEFKERFGV--PRIAEFYAATEGNSgfINFFG-------KPGAIGrnpsllrkvaplalVKYDlESG 269

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  810 VYVLDAN--LHPAPIGVLGEL--HIAGEGLARGYWNrPGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGRTDH 885
Cdd:cd05940   270 EPIRDAEgrCIKVPRGEPGLLisRINPLEPFDGYTD-PAATEKKILRDVF-KKGDAWFNTGDLMRLDGEGFWYFVDRLGD 347

                         410
                  ....*....|....*...
gi 653678460  886 QVKLRGFRIELGEIETTL 903
Cdd:cd05940   348 TFRWKGENVSTTEVAAVL 365
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
 1679-2033 1.19e-14

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 80.35  E-value: 1.19e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSwAAASYPTAGRRGTLAHS-----SVGFDLTmsaLFEPLVSGRGVT 1753
Cdd:PRK08633  778 TFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIE-QISDVFNLRNDDVILSSlpffhSFGLTVT---LWLPLLEGIKVV 853

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1754 LM--PADA-TLADLAEELSGPL---------AY-DYIRLTP---SHLRhLVghwtgqelppaargwVVGGETLDPAlVKQ 1817
Cdd:PRK08633  854 YHpdPTDAlGIAKLVAKHRATIllgtptflrLYlRNKKLHPlmfASLR-LV---------------VAGAEKLKPE-VAD 916

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1818 LLELRPDAEVINHYGPTET--VIGRVVHPVREAG--ELAVDSPLPLGRPLGETRLQVLDA-WLEAVPVGAVGELFIGGDG 1892
Cdd:PRK08633  917 AFEEKFGIRILEGYGATETspVASVNLPDVLAADfkRQTGSKEGSVGMPLPGVAVRIVDPeTFEELPPGEDGLILIGGPQ 996

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1893 IARGYLGRPALTAEkFLPDPagePGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAE---HPGVEQ 1969
Cdd:PRK08633  997 VMKGYLGDPEKTAE-VIKDI---DGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAKalgGEEVVF 1072

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 1970 AVVLVRD----QQLVGWFIPAEDhppvTVSALRRFCAE-QLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK08633 1073 AVTAVPDekkgEKLVVLHTCGAE----DVEELKRAIKEsGLPNLWKPSRYFKVEALPLLGSGKLDLKGL 1137
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
1860-2033 1.36e-14

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 79.26  E-value: 1.36e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1860 GRPL-GETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRWRGDGLLDFV 1938
Cdd:PRK10946  356 GRPMsPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAF--DANG-----FYCSGDLVSIDPDGYITVV 428

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVG----WFIPAEDhpPVTVSALRRFCAEQ-LPEFMVPNQ 2013
Cdd:PRK10946  429 GREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGekscAFLVVKE--PLKAVQLRRFLREQgIAEFKLPDR 506

                         170       180
                  ....*....|....*....|
gi 653678460 2014 WVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK10946  507 VECVDSLPLTAVGKVDKKQL 526
Cyc_NRPS cd19535
Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the ...
 1082-1399 1.37e-14

Cyc (heterocyclization) domain of nonribosomal peptide synthetases (NRPSs); belongs to the Condensation-domain family; Cyc (heterocyclization) domains catalyze two separate reactions in the creation of heterocyclized peptide products in nonribosomal peptide synthesis: amide bond formation followed by intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and a carbonyl carbon on the peptide backbone to form a thiazoline, oxazoline, or methyloxazoline ring. Cyc-domains are homologous to standard NRPS Condensation (C) domains. C-domains typically have a conserved HHxxxD motif at the active site; Cyc-domains have an alternative, conserved DxxxxD active site motif, mutation of the aspartate residues in this motif can abolish or diminish condensation activity. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). There are various subtypes of C-domains such as the LCL-type which catalyzes peptide bond formation between two L-amino acids, the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and Cyc-domains. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain.


Pssm-ID: 380458 [Multi-domain]  Cd Length: 423  Bit Score: 78.68  E-value: 1.37e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1082 PLPLSPMQESIWL---ADQVSAGDS--VYnvplaLRLIGP-LDRGALRRTLNRVVDRHEMLRTRIHpgPDGMPVQVADPA 1155
Cdd:cd19535     1 PFPLTDVQYAYWIgrqDDQELGGVGchAY-----LEFDGEdLDPDRLERAWNKLIARHPMLRAVFL--DDGTQQILPEVP 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1156 ggGAALTERDaapgtdlaqlllaeaalgFTLATEHPLRAVLWRLDER-EHVLLLTAH------------------HV--- 1213
Cdd:cd19535    74 --WYGITVHD------------------LRGLSEEEAEAALEELRERlSHRVLDVERgplfdirlsllpegrtrlHLsid 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1214 --AVDAWSMDLIQRDLTELYaadtghREPELDEPALAQ--ADYaVWQRQSAQRGRYAAELDFWRTELTgavatevagDLP 1289
Cdd:cd19535   134 llVADALSLQILLRELAALY------EDPGEPLPPLELsfRDY-LLAEQALRETAYERARAYWQERLP---------TLP 197

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1290 RP-----ATPGGGGGAVEFA-----LAPRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGR--GNP 1357
Cdd:cd19535   198 PApqlplAKDPEEIKEPRFTrrehrLSAEQWQRLKERARQHGVTPSMVLLTAYAEVLARWSGQPRFLLNLTLFNRlpLHP 277

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 653678460 1358 RLDNLVGCLVNTVVLRGDLSGAPTFHELLRRTHARTADALAH 1399
Cdd:cd19535   278 DVNDVVGDFTSLLLLEVDGSEGQSFLERARRLQQQLWEDLDH 319
GrsT COG3208
Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and ...
 2159-2309 1.78e-14

Surfactin synthase thioesterase subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442441 [Multi-domain]  Cd Length: 237  Bit Score: 75.27  E-value: 1.78e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2159 VFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGR--EAPAETIEDMAAGYLADLRLvQPEGPYVVVGWSLGGTIAF 2236
Cdd:COG3208     9 LFCFPYAGGSASAYRPWAAALPPDIEVLAVQLPGRGDRlgEPPLTSLEELADDLAEELAP-LLDRPFALFGHSMGALLAF 87

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 2237 EMARQLDRLG--EPVQLIL---LEPTLPEVARTIDLhrpardsyLRGADLAERILRLpAGDPEGDRLRAELTRLLEDA 2309
Cdd:COG3208    88 ELARRLERRGrpLPAHLFVsgrRAPHLPRRRRPLHD--------LSDAELLAELRRL-GGTPEEVLADPELLELFLPI 156
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
 1681-1989 2.00e-14

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 78.80  E-value: 2.00e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1681 DPRGLAYSIFTSGSTGQPKGVAVEHIGIV------------------RYLSWaaasYPtagrrgtLAHSsvgFDLTmsal 1742
Cdd:cd17639    86 KPDDLACIMYTSGSTGNPKGVMLTHGNLVagiaglgdrvpellgpddRYLAY----LP-------LAHI---FELA---- 147

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1743 FEPLVSGRGV--------TLMpaDATLA----DLAE----ELSG-PLAYDYIRLT--------PSHLRHLVghWTGQELP 1797
Cdd:cd17639   148 AENVCLYRGGtigygsprTLT--DKSKRgckgDLTEfkptLMVGvPAIWDTIRKGvlaklnpmGGLKRTLF--WTAYQSK 223

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1798 PAARGWVVGGETLDP---ALVKQLL--ELR----------PDA---------EVINHYGPTETVIGrvvHPVREAGELAV 1853
Cdd:cd17639   224 LKALKEGPGTPLLDElvfKKVRAALggRLRymlsggaplsADTqeflnivlcPVIQGYGLTETCAG---GTVQDPGDLET 300

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1854 DSplpLGRPLGETRLQVLDaWLEAvpvGAV-------GELFIGGDGIARGYLGRPALTAEKFLPDpagepgaRMYRTGDL 1926
Cdd:cd17639   301 GR---VGPPLPCCEIKLVD-WEEG---GYStdkppprGEILIRGPNVFKGYYKNPEKTKEAFDGD-------GWFHTGDI 366

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1927 VRWRGDGLLDFVGRVDDQVKLR-GYRIELGEIEARMAEHPGVEQAVVLVRDQQ--LVGWFIPAEDH 1989
Cdd:cd17639   367 GEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYRSNPLVNNICVYADPDKsyPVAIVVPNEKH 432
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
 517-942 3.00e-14

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 78.41  E-value: 3.00e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGA--GAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVT 594
Cdd:cd05927     6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFC 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 DAatadrlgpdDITgLVVLEETDTGG--YPATEPPAVPAgHSAYVIYTSGSTGRPKGVVITRAALDNFLAAM----AERF 668
Cdd:cd05927    86 DA---------GVK-VYSLEEFEKLGkkNKVPPPPPKPE-DLATICYTSGTTGNPKGVMLTHGNIVSNVAGVfkilEILN 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  669 PLTAEDRVLAtttvsfdiaglelYLPL-------------RSGAGV--------VLAD---------------------- 705
Cdd:cd05927   155 KINPTDVYIS-------------YLPLahifervvealflYHGAKIgfysgdirLLLDdikalkptvfpgvprvlnriyd 221

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  706 ---ADTARNPA---ALIDLAGRHRVTLAQATP----TLWQALVPELSGPALAG-IRVLVGGEA-LPAELARRLGDA-GAE 772
Cdd:cd05927   222 kifNKVQAKGPlkrKLFNFALNYKLAELRSGVvrasPFWDKLVFNKIKQALGGnVRLMLTGSApLSPEVLEFLRVAlGCP 301

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  773 VTNMYGPTETTIWSTSgpTSEDSIRRGSIGVPIDNTQVYVLDA---NLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEK 849
Cdd:cd05927   302 VLEGYGQTECTAGATL--TLPGDTSVGHVGGPLPCAEVKLVDVpemNYDAKDPNPRGEVCIRGPNVFSGYYKDPEKTAEA 379

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  850 FLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKL-RGFRIELGEIETTLINAGPVrrAAAVVRedrpGD-LR-- 925
Cdd:cd05927   380 LDEDGW-------LHTGDIGEWLPNGTLKIIDRKKNIFKLsQGEYVAPEKIENIYARSPFV--AQIFVY----GDsLKsf 446

                         490
                  ....*....|....*..
gi 653678460  926 LVAYVIPDgpvaPDALR 942
Cdd:cd05927   447 LVAIVVPD----PDVLK 459
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
 1826-2033 4.63e-14

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 77.88  E-value: 4.63e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1826 EVINHYGPTETVIGRVVHPvREAGELAVdsplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTA 1905
Cdd:PRK05677  353 AICEGYGMTETSPVVSVNP-SQAIQVGT-----IGIPVPSTLCKVIDDDGNELPLGEVGELCVKGPQVMKGYWQRPEATD 426

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1906 EKFlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQ---QLVG 1981
Cdd:PRK05677  427 EIL--DSDG-----WLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQcAAIGVPDEksgEAIK 499

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1982 WFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK05677  500 VFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
 1683-2028 5.13e-14

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 75.76  E-value: 5.13e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1683 RGLAYSIFTSGSTGQPKGVAVEH--------IGIVRYLSWAAASYptagrrgTLAHSSVGFDLTMSALFEPLVSGRGVTL 1754
Cdd:cd17635     1 EDPLAVIFTSGTTGEPKAVLLANktffavpdILQKEGLNWVVGDV-------TYLPLPATHIGGLWWILTCLIHGGLCVT 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MPADATLADLAEELSgplAYDY--IRLTPSHLRHLVGHWTGQ-ELPPAARGWVVGGETLDPAlVKQLLELRPDAEVINHY 1831
Cdd:cd17635    74 GGENTTYKSLFKILT---TNAVttTCLVPTLLSKLVSELKSAnATVPSLRLIGYGGSRAIAA-DVRFIEATGLTNTAQVY 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1832 GPTETviGRVVHPVREAGELAVDSplpLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:cd17635   150 GLSET--GTALCLPTDDDSIEINA---VGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLIDG 224

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1912 pagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ---QLVGWFIPA- 1986
Cdd:cd17635   225 --------WVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVqECACYEISDEefgELVGLAVVAs 296

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 653678460 1987 EDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:cd17635   297 AELDENAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKV 338
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
 514-977 5.85e-14

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 77.08  E-value: 5.85e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  514 DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVV 593
Cdd:cd05939     1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAATAdrlgpdditglvvLEETDTggypaTEPPAVPAG--HSAYV-IYTSGSTGRPKGVVITRAALDNFLAAMAERFPL 670
Cdd:cd05939    81 FNLLDP-------------LLTQSS-----TEPPSQDDVnfRDKLFyIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGM 142

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  671 TAEDRVLATTTVSFDIAG-LELYLPLRSGAGVVLADADTARNpaaLIDLAGRHRVTLAQATPTLWQALVPELSGPALAGI 749
Cdd:cd05939   143 RPEDVVYDCLPLYHSAGGiMGVGQALLHGSTVVIRKKFSASN---FWDDCVKYNCTIVQYIGEICRYLLAQPPSEEEQKH 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  750 RV-LVGGEALPAEL----ARRLGDagAEVTNMYGPTEttiwSTSGPTSEDSiRRGSIG-VPIDNTQVY------------ 811
Cdd:cd05939   220 NVrLAVGNGLRPQIweqfVRRFGI--PQIGEFYGATE----GNSSLVNIDN-HVGACGfNSRILPSVYpirlikvdedtg 292

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  812 --VLDANLH-----PAPIGVLGELHIAGEGLAR--GYWNRpGLTAEKFLPDPFgPPGTRMYRTGDLVRWSAAGDLEYLGR 882
Cdd:cd05939   293 elIRDSDGLcipcqPGEPGLLVGKIIQNDPLRRfdGYVNE-GATNKKIARDVF-KKGDSAFLSGDVLVMDELGYLYFKDR 370

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  883 TDHQVKLRGFRIELGEIETTLINAgpVRRAAAVV-------REDRPGdlrLVAYVIPDGPVAPDALRTELSRTLPDYMIP 955
Cdd:cd05939   371 TGDTFRWKGENVSTTEVEGILSNV--LGLEDVVVygvevpgVEGRAG---MAAIVDPERKVDLDRFSAVLAKSLPPYARP 445

                         490       500
                  ....*....|....*....|..
gi 653678460  956 AVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05939   446 QFIRLLPEVDKTGTFKLQKTDL 467
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
512-977 6.29e-14

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 77.52  E-value: 6.29e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  512 EGDTTLSYRELDERANRLARLLMER-GAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPV 590
Cdd:PRK05620   34 AEQEQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDE 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  591 LVVTDAATADRLGPdditglvVLEETDT-------GGYPATEP--------------------------PAVPAGHSAYV 637
Cdd:PRK05620  114 VIVADPRLAEQLGE-------ILKECPCvravvfiGPSDADSAaahmpegikvysyealldgrstvydwPELDETTAAAI 186

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  638 IYTSGSTGRPKGVVITRAALdnFLAAMAerfpLTAEDRVLATTTVSFdIAGLELY------LPL---RSGAGVVLADADT 708
Cdd:PRK05620  187 CYSTGTTGAPKGVVYSHRSL--YLQSLS----LRTTDSLAVTHGESF-LCCVPIYhvlswgVPLaafMSGTPLVFPGPDL 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  709 ARNPAALIDLAGRHRVtlAQATPTLW-QALVPELSGPA--LAGIRVLVGGEALPAELARRLGDA-GAEVTNMYGPTET-T 783
Cdd:PRK05620  260 SAPTLAKIIATAMPRV--AHGVPTLWiQLMVHYLKNPPerMSLQEIYVGGSAVPPILIKAWEERyGVDVVHVWGMTETsP 337

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  784 IWSTSGPTS------EDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRP----GLTAEKF--- 850
Cdd:PRK05620  338 VGTVARPPSgvsgeaRWAYRVSQGRFPASLEYRIVNDGQVMESTDRNEGEIQVRGNWVTASYYHSPteegGGAASTFrge 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  851 ----LPDPFGPPGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVRED-----RP 921
Cdd:PRK05620  418 dvedANDRFTADG--WLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDdkwgeRP 495

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  922 GDLRLVAYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK05620  496 LAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDL 551
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
 544-976 7.62e-14

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 77.09  E-value: 7.62e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  544 VAVLLPRSADLLVTLLAVIKAGAAYLPI-DPDFP--VERIAYLLSDARPVLVVTDAATAD-------RLGPDDITGLVVL 613
Cdd:PRK12476   95 VAILAPQGIDYVAGFFAAIKAGTIAVPLfAPELPghAERLDTALRDAEPTVVLTTTAAAEavegflrNLPRLRRPRVIAI 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  614 EETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMaerfpltaedrVLATTTVSFDIAGLElYL 693
Cdd:PRK12476  175 DAIPDSAGESFVPVELDTDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQM-----------ILSIDLLDRNTHGVS-WL 242

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  694 PLRSGAGV-------VLADADTARNPAALI----------DLAGRHRVTLAqATPTL---WQA---LVPELSGPALAGIR 750
Cdd:PRK12476  243 PLYHDMGLsmigfpaVYGGHSTLMSPTAFVrrpqrwikalSEGSRTGRVVT-AAPNFayeWAAqrgLPAEGDDIDLSNVV 321

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  751 VLVGGEALPAELARRLGDAGAE-------VTNMYGPTETTIW-STSGPTSE------------------------DSIRR 798
Cdd:PRK12476  322 LIIGSEPVSIDAVTTFNKAFAPyglprtaFKPSYGIAEATLFvATIAPDAEpsvvyldreqlgagravrvaadapNAVAH 401

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  799 GSIGVPIDNTQVYVLDANL-HPAPIGVLGELHIAGEGLARGYWNRPGLTAEKF-------LPDPF----GPPGTRMYRTG 866
Cdd:PRK12476  402 VSCGQVARSQWAVIVDPDTgAELPDGEVGEIWLHGDNIGRGYWGRPEETERTFgaklqsrLAEGShadgAADDGTWLRTG 481

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  867 DLVRWsAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGP-VRR---AAAVVREDRPGDLRLVAYVIP-----DGPVA 937
Cdd:PRK12476  482 DLGVY-LDGELYITGRIADLIVIDGRNHYPQDIEATVAEASPmVRRgyvTAFTVPAEDNERLVIVAERAAgtsraDPAPA 560

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 653678460  938 PDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAA 976
Cdd:PRK12476  561 IDAIRAAVSRRHGLAVADVRLVPAGAIPRTTSGKLARRA 599
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
 1859-2033 7.83e-14

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 76.98  E-value: 7.83e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPDPagepgarMYRTGDLVRWRGDGLLDFV 1938
Cdd:PRK07059  382 IGLPLPSTEVSIRDDDGNDLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTADG-------FFRTGDVGVMDERGYTKIV 454

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ---QLVGWFIPAEDhPPVTVSALRRFCAEQLPEFMVPNQW 2014
Cdd:PRK07059  455 DRKKDMILVSGFNVYPNEIEEVVASHPGVlEVAAVGVPDEhsgEAVKLFVVKKD-PALTEEDVKAFCKERLTNYKRPKFV 533

                         170
                  ....*....|....*....
gi 653678460 2015 VALDAFPLTPHGKVNHRAL 2033
Cdd:PRK07059  534 EFRTELPKTNVGKILRREL 552
PRK08162 PRK08162
acyl-CoA synthetase; Validated
 505-782 9.23e-14

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 76.91  E-value: 9.23e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGA--------------AY-- 568
Cdd:PRK08162   32 PDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAvlntlntrldaasiAFml 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  569 -------LPIDPDF-PVERIAYLLSDARPVLVVTDA----ATADRLGPDDITGLvvLEETD---TGGYPATEPPAVPAGh 633
Cdd:PRK08162  112 rhgeakvLIVDTEFaEVAREALALLPGPKPLVIDVDdpeyPGGRFIGALDYEAF--LASGDpdfAWTLPADEWDAIALN- 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  634 sayviYTSGSTGRPKGVVI-TRAAldnFLAAMAerfpltaedrvlatTTVSFDIAGLELYL-----------------PL 695
Cdd:PRK08162  189 -----YTSGTTGNPKGVVYhHRGA---YLNALS--------------NILAWGMPKHPVYLwtlpmfhcngwcfpwtvAA 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  696 RSGAGVVLADADtarnPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAG-IRVLVGGEALPAELARRLGDAGAE 772
Cdd:PRK08162  247 RAGTNVCLRKVD----PKLIFDLIREHGVTHYCGAPIVLSALInaPAEWRAGIDHpVHAMVAGAAPPAAVIAKMEEIGFD 322

                         330
                  ....*....|
gi 653678460  773 VTNMYGPTET 782
Cdd:PRK08162  323 LTHVYGLTET 332
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
 1532-2033 1.33e-13

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 76.22  E-value: 1.33e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQA-DRTPGwtaLRTGDRSLTFAGLDAQANRLAHALhtGALGAPPvGPeTPVLLLLDRSPELVVAMLAVLKAG 1610
Cdd:PRK13388    4 TIAQLLRDRAgDDTIA---VRYGDRTWTWREVLAEAAARAAAL--IALADPD-RP-LHVGVLLGNTPEMLFWLAAAALGG 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1611 GYFIPVDP---GYPMARLTRIADTvtpALVLTRTGQGGCLPGAEVFGD-VPVVALDRVADRLTAMPDQRPEVTVDPRGLA 1686
Cdd:PRK13388   77 YVLVGLNTtrrGAALAADIRRADC---QLLVTDAEHRPLLDGLDLPGVrVLDVDTPAYAELVAAAGALTPHREVDAMDPF 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1687 YSIFTSGSTGQPKGVAVEHiGIVrylswAAASYPTAGRRG-----------TLAHSSvgfdlTMSALFEP-LVSGRGVTL 1754
Cdd:PRK13388  154 MLIFTSGTTGAPKAVRCSH-GRL-----AFAGRALTERFGltrddvcyvsmPLFHSN-----AVMAGWAPaVASGAAVAL 222

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1755 MP---ADATLADLAE-------ELSGPLAydYIRLTPSH-------LRHLVGHWTGQElppaargwvvggetlDPALVKQ 1817
Cdd:PRK13388  223 PAkfsASGFLDDVRRygatyfnYVGKPLA--YILATPERpddadnpLRVAFGNEASPR---------------DIAEFSR 285

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1818 llelRPDAEVINHYGPTETVIGRVVHPVREAGElavdsplpLGRPL-------GETRLQVLDAWLEAV-----PVGAVGE 1885
Cdd:PRK13388  286 ----RFGCQVEDGYGSSEGAVIVVREPGTPPGS--------IGRGApgvaiynPETLTECAVARFDAHgallnADEAIGE 353

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1886 LF-IGGDGIARGYLGRPALTAEKFlpdpagepgaR--MYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMA 1962
Cdd:PRK13388  354 LVnTAGAGFFEGYYNNPEATAERM----------RhgMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILL 423

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 1963 EHPGVEQAVVL-VRDQ----QLVGWFIPAEDHpPVTVSALRRFCAEQ--LPEFMVPNQ-WVAlDAFPLTPHGKVNHRAL 2033
Cdd:PRK13388  424 RHPAINRVAVYaVPDErvgdQVMAALVLRDGA-TFDPDAFAAFLAAQpdLGTKAWPRYvRIA-ADLPSTATNKVLKREL 500
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
 1548-2028 1.50e-13

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 75.80  E-value: 1.50e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGDRSLTFAGLDAQANRLAHALhtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDpgypmARLTr 1627
Cdd:cd12118    21 TSIVYGDRRYTWRQTYDRCRRLASAL--AALG---ISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALN-----TRLD- 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1628 iADTVtpALVLTRTGqggclpgAEV-FGDVPVVALDRVAdrlTAMPD---QRPEVTVDPRGLAYsifTSGSTGQPKGVAV 1703
Cdd:cd12118    90 -AEEI--AFILRHSE-------AKVlFVDREFEYEDLLA---EGDPDfewIPPADEWDPIALNY---TSGTTGRPKGVVY 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1704 EHIGIvrYLSWAAASYptagrRGTLAHSSV-------------GFDLTMSAlfeplVSGRGVTLMPADAtladlaeelsg 1770
Cdd:cd12118   154 HHRGA--YLNALANIL-----EWEMKQHPVylwtlpmfhcngwCFPWTVAA-----VGGTNVCLRKVDA----------- 210

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1771 PLAYDYIRLtpshlrHLVGHWTGQ--------ELPPAARG---WVV----GGETLDPALVKQLLELRPDaeVINHYGPTE 1835
Cdd:cd12118   211 KAIYDLIEK------HKVTHFCGAptvlnmlaNAPPSDARplpHRVhvmtAGAPPPAAVLAKMEELGFD--VTHVYGLTE 282

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 T---VIGRVVHPvrEAGELAVDSPLPLG-----RPLGETRLQVLDA-WLEAVPVGA--VGELFIGGDGIARGYLGRPALT 1904
Cdd:cd12118   283 TygpATVCAWKP--EWDELPTEERARLKarqgvRYVGLEEVDVLDPeTMKPVPRDGktIGEIVFRGNIVMKGYLKNPEAT 360

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1905 AEKFlpdpagEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQlvgW- 1982
Cdd:cd12118   361 AEAF------RGG--WFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVlEAAVVARPDEK---Wg 429

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1983 -----FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDaFPLTPHGKV 2028
Cdd:cd12118   430 evpcaFVELKEGAKVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKI 479
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
 1529-2033 1.60e-13

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 76.23  E-value: 1.60e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1529 DDRTVHQLVEAQADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHTgaLGappVGPETPVLLLLDRSPELVVAMLAVLK 1608
Cdd:PRK06710   22 DIQPLHKYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQK--LG---VEKGDRVAIMLPNCPQAVIGYYGTLL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1609 AGGYFIPVDPGYPM-----------ARLTRIADTVTPA------------LVLTRTGQGGCLPGAEVFgdvPVVALDR-- 1663
Cdd:PRK06710   97 AGGIVVQTNPLYTEreleyqlhdsgAKVILCLDLVFPRvtnvqsatkiehVIVTRIADFLPFPKNLLY---PFVQKKQsn 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1664 ------------VADRLTAMPDQRPEVTVDPRG-LAYSIFTSGSTGQPKGVAVEHIGIVRY----LSW----------AA 1716
Cdd:PRK06710  174 lvvkvsesetihLWNSVEKEVNTGVEVPCDPENdLALLQYTGGTTGFPKGVMLTHKNLVSNtlmgVQWlynckegeevVL 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1717 ASYPTAGRRGTLAHSSVG------------FDLTMsaLFEPLVSGRgVTLMPADATLadlaeelsgplaydYIRLTPSHL 1784
Cdd:PRK06710  254 GVLPFFHVYGMTAVMNLSimqgykmvlipkFDMKM--VFEAIKKHK-VTLFPGAPTI--------------YIALLNSPL 316

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1785 RhlvghwtgQELPPAARGWVVGGETLDPALVKQLLELRPDAEVINHYGPTETvigrvvHPVREAGEL-AVDSPLPLGRPL 1863
Cdd:PRK06710  317 L--------KEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTES------SPVTHSNFLwEKRVPGSIGVPW 382

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1864 GETRLQVLDAWL-EAVPVGAVGELFIGGDGIARGYLGRPALTAeKFLPDPagepgarMYRTGDLVRWRGDGLLDFVGRVD 1942
Cdd:PRK06710  383 PDTEAMIMSLETgEALPPGEIGEIVVKGPQIMKGYWNKPEETA-AVLQDG-------WLHTGDVGYMDEDGFFYVKDRKK 454

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1943 DQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQ---QLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALD 2018
Cdd:PRK06710  455 DMIVASGFNVYPREVEEVLYEHEKVQEVVTIgVPDPyrgETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRD 534

                         570
                  ....*....|....*
gi 653678460 2019 AFPLTPHGKVNHRAL 2033
Cdd:PRK06710  535 ELPKTTVGKILRRVL 549
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
 518-976 1.61e-13

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 75.80  E-value: 1.61e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  518 SYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDAA 597
Cdd:PRK07768   31 TWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAEDTLRVIGMIGAK 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  598 TA------DRLGPD-DITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPL 670
Cdd:PRK07768  111 AVvvgepfLAAAPVlEEKGIRVLTVADLLAADPIDPVETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEF 190

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  671 TAEDRVLATTTVSFDIAGLELYL--PLRSGAGVVL-ADADTARNPAALIDLAGRHRVTLAqATPTLWQALV-------PE 740
Cdd:PRK07768  191 DVETDVMVSWLPLFHDMGMVGFLtvPMYFGAELVKvTPMDFLRDPLLWAELISKYRGTMT-AAPNFAYALLarrlrrqAK 269

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  741 LSGPALAGIRV-LVGGEALPAELARRLGDAGAE-------VTNMYGPTETTI---WSTSG-------------------- 789
Cdd:PRK07768  270 PGAFDLSSLRFaLNGAEPIDPADVEDLLDAGARfglrpeaILPAYGMAEATLavsFSPCGaglvvdevdadllaalrrav 349

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 PTSEDSIRR-GSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYwnrpgLTAEKFLP--DPFGppgtrMYRTG 866
Cdd:PRK07768  350 PATKGNTRRlATLGPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGY-----LTMDGFIPaqDADG-----WLDTG 419

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  867 DLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAYVIPDGPVAPDALRTEL 945
Cdd:PRK07768  420 DLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVaVRLDAGHSREGFAVAVESNAFEDPAEVRRI 499

                         490       500       510
                  ....*....|....*....|....*....|....*....
gi 653678460  946 SRTLPDYMIPAV--------IVPVPDFPTTPNGKLDRAA 976
Cdd:PRK07768  500 RHQVAHEVVAEVgvrprnvvVLGPGSIPKTPSGKLRRAN 538
PRK07514 PRK07514
malonyl-CoA synthase; Validated
 1548-1974 2.24e-13

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 75.30  E-value: 2.24e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGD-RSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLT 1626
Cdd:PRK07514   19 PFIETPDgLRYTYGDLDAASARLANLLV--ALG---VKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELD 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1627 RIADTVTPALVLTRTGQGGCL-PGAEVFGDVPVVALDR-----VADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKG 1700
Cdd:PRK07514   94 YFIGDAEPALVVCDPANFAWLsKIAAAAGAPHVETLDAdgtgsLLEAAAAAPDDFETVPRGADDLAAILYTSGTTGRSKG 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1701 VAVEH-------IGIVRYLSWAAAS--------YPTAG----RRGTL-AHSSVGFdltmSALFEPlvsGRGVTLMPaDAT 1760
Cdd:PRK07514  174 AMLSHgnllsnaLTLVDYWRFTPDDvlihalpiFHTHGlfvaTNVALlAGASMIF----LPKFDP---DAVLALMP-RAT 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1761 LadlaeeLSG-PLAY----DYIRLTPSHLRHLvghwtgqelppaaRGWVVGGETLDPALVKQLLElRPDAEVINHYGPTE 1835
Cdd:PRK07514  246 V------MMGvPTFYtrllQEPRLTREAAAHM-------------RLFISGSAPLLAETHREFQE-RTGHAILERYGMTE 305

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 TVIgRVVHPV---REAGelAVdsplplGRPLGETRLQVLD-AWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLPD 1911
Cdd:PRK07514  306 TNM-NTSNPYdgeRRAG--TV------GFPLPGVSLRVTDpETGAELPPGEIGMIEVKGPNVFKGYWRMPEKTAEEFRAD 376

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1912 PagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLV 1974
Cdd:PRK07514  377 G-------FFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVvESAVIGV 433
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
 508-974 3.54e-13

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 75.18  E-value: 3.54e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  508 VAVI-EGD-----TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIA 581
Cdd:PRK00174   84 VAIIwEGDdpgdsRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGGFSAEALA 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  582 YLLSDARPVLVVT-----------------DAATADrlgPDDITGLVVLEETD----------------TGGYPAT-EPP 627
Cdd:PRK00174  164 DRIIDAGAKLVITadegvrggkpiplkanvDEALAN---CPSVEKVIVVRRTGgdvdwvegrdlwwhelVAGASDEcEPE 240

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  628 AVPAGHSAYVIYTSGSTGRPKGVVITRAALdNFLAAMaerfpltaedrvlaTTTVSFDIAGLE----------------- 690
Cdd:PRK00174  241 PMDAEDPLFILYTSGSTGKPKGVLHTTGGY-LVYAAM--------------TMKYVFDYKDGDvywctadvgwvtghsyi 305

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  691 LYLPLRSGAGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLwqalvpelsgpalagIRVLVG-GEALPAE--LA--R 764
Cdd:PRK00174  306 VYGPLANGATTLMFEgVPNYPDPGRFWEVIDKHKVTIFYTAPTA---------------IRALMKeGDEHPKKydLSslR 370

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  765 RLGDAG------------AEVTNMYGPTETTIWSTS----------GPTSedsIRRGSIGVPIDNTQVYVLDANLHPAPI 822
Cdd:PRK00174  371 LLGSVGepinpeawewyyKVVGGERCPIVDTWWQTEtggimitplpGATP---LKPGSATRPLPGIQPAVVDEEGNPLEG 447

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  823 GVLGELHIAGE--GLARGYWNRPgltaEKFLPDPFGP-PGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEI 899
Cdd:PRK00174  448 GEGGNLVIKDPwpGMMRTIYGDH----ERFVKTYFSTfKG--MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEI 521

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  900 ETTLInAGPVRRAAAVVreDRPGDLR---LVAYVIPDGPVAP-DALRTEL----SRTLPDYMIPAVIVPVPDFPTTPNGK 971
Cdd:PRK00174  522 ESALV-AHPKVAEAAVV--GRPDDIKgqgIYAFVTLKGGEEPsDELRKELrnwvRKEIGPIAKPDVIQFAPGLPKTRSGK 598


                  ...
gi 653678460  972 LDR 974
Cdd:PRK00174  599 IMR 601
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
 518-977 6.25e-13

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 74.04  E-value: 6.25e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  518 SYRELDERANRLARLL-----MERGagaeTFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLV 592
Cdd:cd05928    43 SFRELGSLSRKAANVLsgacgLQRG----DRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCI 118

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  593 VTDAATADRLG------PDDITGLVVLEETDTG--------GYPATEPPAVPAGHS-AYVIY-TSGSTGRPKGVVITRAA 656
Cdd:cd05928   119 VTSDELAPEVDsvasecPSLKTKLLVSEKSRDGwlnfkellNEASTEHHCVETGSQePMAIYfTSGTTGSPKMAEHSHSS 198

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  657 LDNFLAAMAERF-PLTAEDRVLATTTVSFDIAGL-ELYLPLRSGAgVVLADADTARNPAALIDLAGRHRVTLAQATPTLW 734
Cdd:cd05928   199 LGLGLKVNGRYWlDLTASDIMWNTSDTGWIKSAWsSLFEPWIQGA-CVFVHHLPRFDPLVILKTLSSYPITTFCGAPTVY 277

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  735 QALVPE-LSGPALAGIR-VLVGGEAL-PAELARRLGDAGAEVTNMYGPTETTIwsTSGPTSEDSIRRGSIGVPIDNTQVY 811
Cdd:cd05928   278 RMLVQQdLSSYKFPSLQhCVTGGEPLnPEVLEKWKAQTGLDIYEGYGQTETGL--ICANFKGMKIKPGSMGKASPPYDVQ 355

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  812 VLDANLHPAPIGVLGELHIAGE-----GLARGYWNRPGLTAEKFLPDpfgppgtrMYRTGDLVRWSAAGDLEYLGRTDHQ 886
Cdd:cd05928   356 IIDDNGNVLPPGTEGDIGIRVKpirpfGLFSGYVDNPEKTAATIRGD--------FYLTGDRGIMDEDGYFWFMGRADDV 427

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  887 VKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPV---APDALRTEL-----SRTLPdYMIPAVI 958
Cdd:cd05928   428 INSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFlshDPEQLTKELqqhvkSVTAP-YKYPRKV 506

                         490
                  ....*....|....*....
gi 653678460  959 VPVPDFPTTPNGKLDRAAL 977
Cdd:cd05928   507 EFVQELPKTVTGKIQRNEL 525
PRK08308 PRK08308
acyl-CoA synthetase; Validated
 1917-2034 7.18e-13

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 73.15  E-value: 7.18e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1917 GARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFIPAE--DHPPVTV 1994
Cdd:PRK08308  289 GDKEIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKviSHEEIDP 368

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 653678460 1995 SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALP 2034
Cdd:PRK08308  369 VQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLLE 408
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
 511-977 1.00e-12

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 73.25  E-value: 1.00e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  511 IEGDTT-LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP 589
Cdd:PRK06018   33 VEGPIVrTTYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAED 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  590 VLVVTD-------AATADRLgpDDITGLVVLeeTDTGGYPATEPP-------------------AVPAGHSAYVIYTSGS 643
Cdd:PRK06018  113 RVVITDltfvpilEKIADKL--PSVERYVVL--TDAAHMPQTTLKnavayeewiaeadgdfawkTFDENTAAGMCYTSGT 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  644 TGRPKGVVITRAA--LDNFLAAMAERFPLTAEDRVLATTTVSFDIA-GLELYLPlRSGAGVVLADADTarNPAALIDLAG 720
Cdd:PRK06018  189 TGDPKGVLYSHRSnvLHALMANNGDALGTSAADTMLPVVPLFHANSwGIAFSAP-SMGTKLVMPGAKL--DGASVYELLD 265

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQATPTLWQALVPELSG-----PALAgiRVLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSgptseds 795
Cdd:PRK06018  266 TEKVTFTAGVPTVWLMLLQYMEKeglklPHLK--MVVCGGSAMPRSMIKAFEDMGVEVRHAWGMTEMSPLGTL------- 336

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  796 irrGSIGVPIDNTQVyvlDANLHP------APIGV------------------LGELHIAGEGLARGYWNRPG--LTAEK 849
Cdd:PRK06018  337 ---AALKPPFSKLPG---DARLDVlqkqgyPPFGVemkitddagkelpwdgktFGRLKVRGPAVAAAYYRVDGeiLDDDG 410

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  850 FlpdpfgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLR--LV 927
Cdd:PRK06018  411 F------------FDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERplLI 478

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 653678460  928 AYVIPDGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK06018  479 VQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTAL 528
PRK05857 PRK05857
fatty acid--CoA ligase;
1532-2039 1.59e-12

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 72.73  E-value: 1.59e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1532 TVHQLVEAQADRTPGWTALRTGD--RSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKA 1609
Cdd:PRK05857   15 TVLDRVFEQARQQPEAIALRRCDgtSALRYRELVAEVGGLA-----ADLRAQSVSRGSRVLVISDNGPETYLSVLACAKL 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1610 GGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQG-GCLPGAEVFGDVPVVALDRVADRL-------TAMPDQRPEVTVD 1681
Cdd:PRK05857   90 GAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKmASSAVPEALHSIPVIAVDIAAVTResehsldAASLAGNADQGSE 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 pRGLAYsIFTSGSTGQPKGV--------AVEHIGIVRYLSWAaasyptagrrgtlahSSVGFDLTMSALFEPLVSG---- 1749
Cdd:PRK05857  170 -DPLAM-IFTSGTTGEPKAVllanrtffAVPDILQKEGLNWV---------------TWVVGETTYSPLPATHIGGlwwi 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1750 -----RGVTLMPADATLADLAEELSGPlAYDYIRLTPSHLRHLVGHW-TGQELPPAARGWVVGGETLDPALVK--QLLEL 1821
Cdd:PRK05857  233 ltclmHGGLCVTGGENTTSLLEILTTN-AVATTCLVPTLLSKLVSELkSANATVPSLRLVGYGGSRAIAADVRfiEATGV 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1822 RpDAEVinhYGPTETVIGRVVHPVrEAGELAVDSPLPLGRPLG--ETRLQVLDAWLEAVPVGA----VGELFIGGDGIAR 1895
Cdd:PRK05857  312 R-TAQV---YGLSETGCTALCLPT-DDGSIVKIEAGAVGRPYPgvDVYLAATDGIGPTAPGAGpsasFGTLWIKSPANML 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1896 GYLGRPALTAEKFLPDpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEaRMAEH-PGV-EQAVVL 1973
Cdd:PRK05857  387 GYWNNPERTAEVLIDG--------WVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVD-RIAEGvSGVrEAACYE 457

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1974 VRDQQ---LVGW-FIPAEDHPPVTVSALRRFCAEQL---PEFMV-PNQWVALDAFPLTPHGKVNHRALPGPTSG 2039
Cdd:PRK05857  458 IPDEEfgaLVGLaVVASAELDESAARALKHTIAARFrreSEPMArPSTIVIVTDIPRTQSGKVMRASLAAAATA 531
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
 631-868 1.84e-12

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 72.52  E-value: 1.84e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  631 AGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYL-PLRSGAG-VVLADADT 708
Cdd:cd05908   105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLaPLIAGMNqYLMPTRLF 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  709 ARNPAALIDLAGRHRVTLAQ----ATPTLWQALVPELSGPA-LAGIRVLV-GGEALPAELARRLGDAGAE-------VTN 775
Cdd:cd05908   185 IRRPILWLKKASEHKATIVSspnfGYKYFLKTLKPEKANDWdLSSIRMILnGAEPIDYELCHEFLDHMSKyglkrnaILP 264

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  776 MYGPTETTIWSTSGPTSEDSI-----RRG---------------------SIGVPIDNTQVYVLDANLHPAPIGVLGELH 829
Cdd:cd05908   265 VYGLAEASVGASLPKAQSPFKtitlgRRHvthgepepevdkkdsecltfvEVGKPIDETDIRICDEDNKILPDGYIGHIQ 344

                         250       260       270
                  ....*....|....*....|....*....|....*....
gi 653678460  830 IAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDL 868
Cdd:cd05908   345 IRGKNVTPGYYNNPEATAKVFTDDGW-------LKTGDL 376
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
 508-977 2.17e-12

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 72.32  E-value: 2.17e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  508 VAVIEGDT--TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLS 585
Cdd:PLN02330   45 VAFVEAVTgkAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAE 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  586 DARPVLVVTDAATADR----------LGPDDITGLV----VLEETDTGGYPA-------TEPPAVPaghsayviYTSGST 644
Cdd:PLN02330  125 AAGAKLIVTNDTNYGKvkglglpvivLGEEKIEGAVnwkeLLEAADRAGDTSdneeilqTDLCALP--------FSSGTT 196

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  645 GRPKGVVITRAaldNFLAAMAERFPLTAEDRVLATTTVS----FDIAGLE--LYLPLRS-GAGVVLADADTARNPAALId 717
Cdd:PLN02330  197 GISKGVMLTHR---NLVANLCSSLFSVGPEMIGQVVTLGlipfFHIYGITgiCCATLRNkGKVVVMSRFELRTFLNALI- 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  718 lagRHRVTLAQATPTLWQALVP-----ELSGPALAGIRVLVGGEALPAEL--ARRLGDAGAEVTNMYGPTE-TTIWSTSG 789
Cdd:PLN02330  273 ---TQEVSFAPIVPPIILNLVKnpiveEFDLSKLKLQAIMTAAAPLAPELltAFEAKFPGVQVQEAYGLTEhSCITLTHG 349

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  790 -PTSEDSI-RRGSIGVPIDNTQVYVLDA-NLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTG 866
Cdd:PLN02330  350 dPEKGHGIaKKNSVGFILPNLEVKFIDPdTGRSLPKNTPGELCVRSQCVMQGYYNNKEETDRTIDEDGW-------LHTG 422

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  867 DLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAV-VREDRPGDLRLVAYVI-PDGPVAPDALRTE 944
Cdd:PLN02330  423 DIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVpLPDEEAGEIPAACVVInPKAKESEEDILNF 502

                         490       500       510
                  ....*....|....*....|....*....|...
gi 653678460  945 LSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PLN02330  503 VAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLL 535
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
 487-977 2.78e-12

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 71.79  E-value: 2.78e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  487 PLPAASLRERFQQWCRR---TPGAVAVIEGDT--TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAV 561
Cdd:cd17642    10 PLEDGTAGEQLHKAMKRyasVPGTIAFTDAHTgvNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAG 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  562 IKAGAAYLPIDPDFPVERIAYLLSDARPVLVVTDAATADRLGP-----DDITGLVVLE-ETDTGGYPATE---PPAVPAG 632
Cdd:cd17642    90 LFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSKKGLQKVLNvqkklKIIKTIIILDsKEDYKGYQCLYtfiTQNLPPG 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  633 HSAY---------------VIYTSGSTGRPKGVVIT-RAALDNFLAAMAERF-----PLTAEDRVL-------ATTTVSF 684
Cdd:cd17642   170 FNEYdfkppsfdrdeqvalIMNSSGSTGLPKGVQLThKNIVARFSHARDPIFgnqiiPDTAILTVIpfhhgfgMFTTLGY 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  685 DIAGLELYLPLRSGAGVVLAdadtarnpaALIDlagrHRVTLAQATPTLWQALV--PELSGPALAGIRVLVGGEAlpaEL 762
Cdd:cd17642   250 LICGFRVVLMYKFEEELFLR---------SLQD----YKVQSALLVPTLFAFFAksTLVDKYDLSNLHEIASGGA---PL 313

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  763 ARRLGDAGAEVTNM------YGPTETTiwSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPApIGV--LGELHIAGEG 834
Cdd:cd17642   314 SKEVGEAVAKRFKLpgirqgYGLTETT--SAILITPEGDDKPGAVGKVVPFFYAKVVDLDTGKT-LGPneRGELCVKGPM 390

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  835 LARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAA- 913
Cdd:cd17642   391 IMKGYVNNPEATKALIDKDGW-------LHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGv 463

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 653678460  914 AVVREDRPGDLRLVAYVIPDGpvapdalRTELSRTLPDYMIPAV---------IVPVPDFPTTPNGKLDRAAL 977
Cdd:cd17642   464 AGIPDEDAGELPAAVVVLEAG-------KTMTEKEVMDYVASQVstakrlrggVKFVDEVPKGLTGKIDRRKI 529
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
 516-889 4.52e-12

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 71.33  E-value: 4.52e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLL-MERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVT 594
Cdd:cd17632    67 TITYAELWERVGAVAAAHdPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAV 146

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 DAAT------------------------------------ADRLGPDDITGLVVLEETDTGGYPATEPPAVPAGHS---A 635
Cdd:cd17632   147 SAEHldlaveavleggtpprlvvfdhrpevdahraalesaRERLAAVGIPVTTLTLIAVRGRDLPPAPLFRPEPDDdplA 226

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  636 YVIYTSGSTGRPKGVVITRaaldNFLAAMAERFPLTAEDRVLATTTVSF----DIAG-LELYLPL-RSGAGVVLADADTa 709
Cdd:cd17632   227 LLIYTSGSTGTPKGAMYTE----RLVATFWLKVSSIQDIRPPASITLNFmpmsHIAGrISLYGTLaRGGTAYFAAASDM- 301

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  710 rnpAALIDLAGRHRVTLAQATPTLW----QALVPELSGPALAGIRVLVGGEALPAELARRL----------GDA------ 769
Cdd:cd17632   302 ---STLFDDLALVRPTELFLVPRVCdmlfQRYQAELDRRSVAGADAETLAERVKAELRERVlggrllaavcGSAplsaem 378

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  770 --------GAEVTNMYGPTETTIwstsgptsedSIRRGSIGVP--IDNTQV------YVLDANLHPApigvlGELHIAGE 833
Cdd:cd17632   379 kafmesllDLDLHDGYGSTEAGA----------VILDGVIVRPpvLDYKLVdvpelgYFRTDRPHPR-----GELLVKTD 443

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  834 GLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKL 889
Cdd:cd17632   444 TLFPGYYKRPEVTAEVFDEDGF-------YRTGDVMAELGPDRLVYVDRRNNVLKL 492
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
 1831-2029 5.06e-12

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 69.61  E-value: 5.06e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1831 YGPTETVIGRVVHPVREAgelavdsPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLP 1910
Cdd:cd17637   143 YGQTETSGLVTLSPYRER-------PGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTFRN 215

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1911 DpagepgarMYRTGDLVRWRGDGLLDFVGRV--DDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQLvGWFIPA- 1986
Cdd:cd17637   216 G--------WHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIgVPDPKW-GEGIKAv 286

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 653678460 1987 -EDHPPVTVSA--LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVN 2029
Cdd:cd17637   287 cVLKPGATLTAdeLIEFVGSRIARYKKPRYVVFVEALPKTADGSID 332
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
 1690-1972 5.27e-12

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 70.96  E-value: 5.27e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 FTSGSTGQPKgvAVEHigivRYLSWAAASYPTAGRRGTLAHSSVGFDLT--------MSALFEPLVSGRGV--TLMPADA 1759
Cdd:cd05928   181 FTSGTTGSPK--MAEH----SHSSLGLGLKVNGRYWLDLTASDIMWNTSdtgwiksaWSSLFEPWIQGACVfvHHLPRFD 254

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1760 TLADLAEELSGPLAYdyIRLTPSHLRHLVGhwtgQELP----PAARGWVVGGETLDPALVKQLLElRPDAEVINHYGPTE 1835
Cdd:cd05928   255 PLVILKTLSSYPITT--FCGAPTVYRMLVQ----QDLSsykfPSLQHCVTGGEPLNPEVLEKWKA-QTGLDIYEGYGQTE 327

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 TVIgrvVHPVREAGELavdSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGD-----GIARGYLGRPALTAEKFLP 1910
Cdd:cd05928   328 TGL---ICANFKGMKI---KPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRVKpirpfGLFSGYVDNPEKTAATIRG 401

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 653678460 1911 DpagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVV 1972
Cdd:cd05928   402 D--------FYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVvESAVV 456
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
 1541-2019 5.55e-12

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 70.67  E-value: 5.55e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY 1620
Cdd:PRK09029   13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFA--QQG---VVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQL 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 PMARLTRIADTVTPALVLTRTGQGgclpgaevfgdvpvvALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKg 1700
Cdd:PRK09029   88 PQPLLEELLPSLTLDFALVLEGEN---------------TFSALTSLHLQLVEGAHAVAWQPQRLATMTLTSGSTGLPK- 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1701 vAVEHigivrylswaaasypTAGrrgtlAH--SSVG------FDLTMSALFE-PL--VSGRGVT---LMpADATL----- 1761
Cdd:PRK09029  152 -AAVH---------------TAQ-----AHlaSAEGvlslmpFTAQDSWLLSlPLfhVSGQGIVwrwLY-AGATLvvrdk 209

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1762 ADLAEELSGplaYDYIRLTPSHLRHLVGHWtgqELPPAARGWVVGGETLDPALVKQllelrpdAEVIN-----HYGPTE- 1835
Cdd:PRK09029  210 QPLEQALAG---CTHASLVPTQLWRLLDNR---SEPLSLKAVLLGGAAIPVELTEQ-------AEQQGircwcGYGLTEm 276

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 --TVIgrvvhpVREAGELA-VDSPLPlGRplgETRLqvldawleavpVGavGELFIGGDGIARGYLGRPALTAekfLPDP 1912
Cdd:PRK09029  277 asTVC------AKRADGLAgVGSPLP-GR---EVKL-----------VD--GEIWLRGASLALGYWRQGQLVP---LVND 330

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1913 AGepgarMYRTGDLVRWRGDGLLdFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLvrdqqlvgwfiPAED---- 1988
Cdd:PRK09029  331 EG-----WFATRDRGEWQNGELT-ILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVV-----------PVADaefg 393

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|
gi 653678460 1989 HPPV---------TVSALRRFCAEQLPEFMVPNQWVALDA 2019
Cdd:PRK09029  394 QRPVavvesdseaAVVNLAEWLQDKLARFQQPVAYYLLPP 433
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
 1493-1705 6.10e-12

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 71.06  E-value: 6.10e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1493 AAAVRTPELAVTRLAITSESDTVRTSRwgrSEQHVGD-DRTVHQLVEAQADRTPG--WTALRTGD---RSLTFAGLDAQA 1566
Cdd:PRK08180    3 PARYRPVAFAPPAVEVERRADGTIYLR---SAEPLGDyPRRLTDRLVHWAQEAPDrvFLAERGADggwRRLTYAEALERV 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1567 NRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY-----PMARLTRIADTVTPALVLTRT 1641
Cdd:PRK08180   80 RAIAQALL--DRG---LSAERPLMILSGNSIEHALLALAAMYAGVPYAPVSPAYslvsqDFGKLRHVLELLTPGLVFADD 154

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1642 GQ--GGCLpGAEVFGDVPVVAL-----DRVADRLTAMPDQRPEVTVD-------PRGLAYSIFTSGSTGQPKGVAVEH 1705
Cdd:PRK08180  155 GAafARAL-AAVVPADVEVVAVrgavpGRAATPFAALLATPPTAAVDaahaavgPDTIAKFLFTSGSTGLPKAVINTH 231
PLN02654 PLN02654
acetate-CoA ligase
 514-977 6.46e-12

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 71.08  E-value: 6.46e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  514 DTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVV 593
Cdd:PLN02654  118 DASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVI 197

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TdaATADRLGP-----DDITGLVVLEETDTG-----------------------------------GYPAT-EPPAVPAG 632
Cdd:PLN02654  198 T--CNAVKRGPktinlKDIVDAALDESAKNGvsvgicltyenqlamkredtkwqegrdvwwqdvvpNYPTKcEVEWVDAE 275

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  633 HSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAER-FPLTAEDRVLATTTVSFdIAGLEL--YLPLRSGAGVVLAD-ADT 708
Cdd:PLN02654  276 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYaFDYKPTDVYWCTADCGW-ITGHSYvtYGPMLNGATVLVFEgAPN 354

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  709 ARNPAALIDLAGRHRVTLAQATPTLWQALVPELSGP----ALAGIRVL--VGGEALPAelARR-----LGDAGAEVTNMY 777
Cdd:PLN02654  355 YPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYvtrhSRKSLRVLgsVGEPINPS--AWRwffnvVGDSRCPISDTW 432

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  778 GPTETTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANlhpapiGVLGELHIAGEGLARGYWnrPGL------TAEKFL 851
Cdd:PLN02654  433 WQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEK------GKEIEGECSGYLCVKKSW--PGAfrtlygDHERYE 504

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  852 PDPFGP-PGtrMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYV 930
Cdd:PLN02654  505 TTYFKPfAG--YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFV 582

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460  931 -IPDGPVAPDALRTELSRTLPD----YMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PLN02654  583 tLVEGVPYSEELRKSLILTVRNqigaFAAPDKIHWAPGLPKTRSGKIMRRIL 634
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
 1530-1947 6.95e-12

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 70.85  E-value: 6.95e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1530 DRTVHQLVEAQADRTPG--WTALRTGD----RSLTFAgldaQANRLAHALhTGALGAPPVGPETPVLLLLDRSPELVVAM 1603
Cdd:PRK12582   48 PRSIPHLLAKWAAEAPDrpWLAQREPGhgqwRKVTYG----EAKRAVDAL-AQALLDLGLDPGRPVMILSGNSIEHALMT 122

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1604 LAVLKAGGYFIPVDPGYPM-----ARLTRIADTVTPALVLTRTGQ--GGCLPGAEvFGDVPVVALDRVADRLTAMPDQ-- 1674
Cdd:PRK12582  123 LAAMQAGVPAAPVSPAYSLmshdhAKLKHLFDLVKPRVVFAQSGApfARALAALD-LLDVTVVHVTGPGEGIASIAFAdl 201

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1675 -----RPEV-----TVDPRGLAYSIFTSGSTGQPKGVAVEHigivRYLSWAAASYPTAGRRGTLAHSSVGFDL-----TM 1739
Cdd:PRK12582  202 aatppTAAVaaaiaAITPDTVAKYLFTSGSTGMPKAVINTQ----RMMCANIAMQEQLRPREPDPPPPVSLDWmpwnhTM 277

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1740 --SALFEPLVSGRGvTL-------MPA--DATLADLAEelSGPLAYDYirlTPSHLRHLVGHwtgQELPPAARGWVV--- 1805
Cdd:PRK12582  278 ggNANFNGLLWGGG-TLyiddgkpLPGmfEETIRNLRE--ISPTVYGN---VPAGYAMLAEA---MEKDDALRRSFFknl 348

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1806 -----GGETLDPALVKQLLELRPDAE-----VINHYGPTETV-IGRVVHPVREAGELavdsplpLGRPLGETRLQVldaw 1874
Cdd:PRK12582  349 rlmayGGATLSDDLYERMQALAVRTTghripFYTGYGATETApTTTGTHWDTERVGL-------IGLPLPGVELKL---- 417

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1875 leaVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRW-------RGdglLDFVGRVDDQVKL 1947
Cdd:PRK12582  418 ---APVGDKYEVRVKGPNVTPGYHKDPELTAAAF--DEEG-----FYRLGDAARFvdpddpeKG---LIFDGRVAEDFKL 484
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
 1682-2028 9.59e-12

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 70.21  E-value: 9.59e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 PRGLAYSIFTSGSTGQPKGVAVEHIG-IVRYLS-WAAASYP-------TA------GRRGTLAHSSVG--------FDlt 1738
Cdd:PLN02860  171 PDDAVLICFTSGTTGRPKGVTISHSAlIVQSLAkIAIVGYGeddvylhTAplchigGLSSALAMLMVGachvllpkFD-- 248

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1739 MSALFEPLVSGRGVTLMPADATLADLAEelsgplaydYIRLTpshlrhlvGHWTGQelpPAARGWVVGGETLDPALVKQL 1818
Cdd:PLN02860  249 AKAALQAIKQHNVTSMITVPAMMADLIS---------LTRKS--------MTWKVF---PSVRKILNGGGSLSSRLLPDA 308

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1819 LELRPDAEVINHYGPTETVIGRVVHPVREAgelAVDSPLPLGRPLGETRLQVLDAwLEAVPVGAVG---ELFIGGDGIAR 1895
Cdd:PLN02860  309 KKLFPNAKLFSAYGMTEACSSLTFMTLHDP---TLESPKQTLQTVNQTKSSSVHQ-PQGVCVGKPAphvELKIGLDESSR 384

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1896 -------------GYLGRPALTAEKfLPDPAgepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMA 1962
Cdd:PLN02860  385 vgriltrgphvmlGYWGQNSETASV-LSNDG------WLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLS 457

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1963 EHPGVEQAVVL-VRDQQLV-----------GWFIPAEDHP----PVTVSA--LRRFCAEQ-LPEFMVPN---QWvaLDAF 2020
Cdd:PLN02860  458 QHPGVASVVVVgVPDSRLTemvvacvrlrdGWIWSDNEKEnakkNLTLSSetLRHHCREKnLSRFKIPKlfvQW--RKPF 535


                  ....*...
gi 653678460 2021 PLTPHGKV 2028
Cdd:PLN02860  536 PLTTTGKI 543
PP-binding pfam00550
Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached ...
 2056-2113 1.63e-11

Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.


Pssm-ID: 425746 [Multi-domain]  Cd Length: 62  Bit Score: 61.43  E-value: 1.63e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460  2056 QLLAELWSTVLG--TDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVR 2113
Cdd:pfam00550    1 ERLRELLAEVLGvpAEEIDPDTDLFDLGLDSLLAVELIARLEEEfGVEIPPSDLFEHPTLA 61
AcpA COG3433
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ...
 782-1036 2.31e-11

Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442659 [Multi-domain]  Cd Length: 295  Bit Score: 67.08  E-value: 2.31e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  782 TTIWSTSGPTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFLPDPFGPPGTR 861
Cdd:COG3433     1 IAIATPPPAPPTPDEPPPVIPPAIVQARALLLIVDLQGYFGGFGGEGGLLGAGLLLRIRLLAAAARAPFIPVPYPAQPGR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  862 MYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIPDGPVAPDAL 941
Cdd:COG3433    81 QADDLRLLLRRGLGPGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAALRGAGVGLLLIVGAVAALDGLAAAA 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  942 RTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALPAPDY------GTRSTARPPRDDRERFLCTAFAEVLGLP--EVA 1013
Cdd:COG3433   161 ALAALDKVPPDVVAASAVVALDALLLLALKVVARAAPALAAaeallaAASPAPALETALTEEELRADVAELLGVDpeEID 240

                         250       260
                  ....*....|....*....|...
gi 653678460 1014 VSDNFFELGGHSLLAFRLLARIR 1036
Cdd:COG3433   241 PDDNLFDLGLDSIRLMQLVERWR 263
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
 511-977 2.32e-11

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 68.96  E-value: 2.32e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  511 IEGDT-TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARP 589
Cdd:PRK07008   33 VEGDIhRYTYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAED 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  590 VLVVTDA----------------------ATADRLgPDDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRP 647
Cdd:PRK07008  113 RYVLFDLtflplvdalapqcpnvkgwvamTDAAHL-PAGSTPLLCYETLVGAQDGDYDWPRFDENQASSLCYTSGTTGNP 191

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  648 KGVVITR--AALDNFLAAMAERFPLTAEDRVLATTTVsFDIA--GLELYLPLrSGAGVVLADADTarNPAALIDLAGRHR 723
Cdd:PRK07008  192 KGALYSHrsTVLHAYGAALPDAMGLSARDAVLPVVPM-FHVNawGLPYSAPL-TGAKLVLPGPDL--DGKSLYELIEAER 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  724 VTLAQATPTLWQALVPELSGpalAGI------RVLVGGEALPAELARRLGDA-GAEVTNMYGPTE--------TTIWSTS 788
Cdd:PRK07008  268 VTFSAGVPTVWLGLLNHMRE---AGLrfstlrRTVIGGSACPPAMIRTFEDEyGVEVIHAWGMTEmsplgtlcKLKWKHS 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  789 G-PTSEDSIRRGSIGVPIDNTQVYVLDANLHPAPI-GV-LGELHIAGEGLARGYWNRPGltaekflpdpfGPPGTRMYRT 865
Cdd:PRK07008  345 QlPLDEQRKLLEKQGRVIYGVDMKIVGDDGRELPWdGKaFGDLQVRGPWVIDRYFRGDA-----------SPLVDGWFPT 413

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  866 GDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVR-----EDRPgdlrLVAYVI-PDGPVAPD 939
Cdd:PRK07008  414 GDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACahpkwDERP----LLVVVKrPGAEVTRE 489

                         490       500       510
                  ....*....|....*....|....*....|....*...
gi 653678460  940 ALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:PRK07008  490 ELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKL 527
PRK07867 PRK07867
acyl-CoA synthetase; Validated
 1528-2033 2.69e-11

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 68.94  E-value: 2.69e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1528 GDDRTVHQLVEAQADrtpgwtalrTGDRSLTFAG-LDAQANRLAHALHTGALGAPPVGPETP--VLLLLDRSPELVVAML 1604
Cdd:PRK07867    2 SSAPTVAELLLPLAE---------DDDRGLYFEDsFTSWREHIRGSAARAAALRARLDPTRPphVGVLLDNTPEFSLLLG 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1605 AVLKAGGYFIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfGDVPVVALDRV--ADRLTAMPDQRPE-VTVD 1681
Cdd:PRK07867   73 AAALSGIVPVGLNPTRRGAALARDIAHADCQLVLTESAHAELLDGLD--PGVRVINVDSPawADELAAHRDAEPPfRVAD 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 PRGLAYSIFTSGSTGQPKGVAVEHIGIvrylswAAASYPTAGRRGT-----------LAHSSvgfdlTMSALFEPLVSGR 1750
Cdd:PRK07867  151 PDDLFMLIFTSGTSGDPKAVRCTHRKV------ASAGVMLAQRFGLgpddvcyvsmpLFHSN-----AVMAGWAVALAAG 219

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1751 GVTLMP----ADATLADL-------AEELSGPLAydYIRLTPshlrhlvghwtgqELPPAARG---WVVGGETLDPALVK 1816
Cdd:PRK07867  220 ASIALRrkfsASGFLPDVrrygatyANYVGKPLS--YVLATP-------------ERPDDADNplrIVYGNEGAPGDIAR 284

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1817 qlLELRPDAEVINHYGPTETVIGRVVHPVREAGELAvdsPLPLGrplgetrLQVLDA-WLEAVPVG------------AV 1883
Cdd:PRK07867  285 --FARRFGCVVVDGFGSTEGGVAITRTPDTPPGALG---PLPPG-------VAIVDPdTGTECPPAedadgrllnadeAI 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1884 GELF-IGGDGIARGYLGRPALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMA 1962
Cdd:PRK07867  353 GELVnTAGPGGFEGYYNDPEADAERM--------RGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILL 424

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1963 EHPGVEQAVVL------VRDQQLVGwfIPAEDHPPVTVSALRRFCAEQlPEFMvPNQWVAL----DAFPLTPHGKVNHRA 2032
Cdd:PRK07867  425 RYPDATEVAVYavpdpvVGDQVMAA--LVLAPGAKFDPDAFAEFLAAQ-PDLG-PKQWPSYvrvcAELPRTATFKVLKRQ 500


                  .
gi 653678460 2033 L 2033
Cdd:PRK07867  501 L 501
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
1859-2033 4.05e-11

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 68.16  E-value: 4.05e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEkFLPDPagepgarMYRTGDLVRWRGDGLLDFV 1938
Cdd:PRK08974  380 IGLPVPSTEIKLVDDDGNEVPPGEPGELWVKGPQVMLGYWQRPEATDE-VIKDG-------WLATGDIAVMDEEGFLRIV 451

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQ---QLVGWFIPAEDhPPVTVSALRRFCAEQLPEFMVPNQW 2014
Cdd:PRK08974  452 DRKKDMILVSGFNVYPNEIEDVVMLHPKVlEVAAVGVPSEvsgEAVKIFVVKKD-PSLTEEELITHCRRHLTGYKVPKLV 530

                         170
                  ....*....|....*....
gi 653678460 2015 VALDAFPLTPHGKVNHRAL 2033
Cdd:PRK08974  531 EFRDELPKSNVGKILRREL 549
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
 516-977 4.56e-11

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 67.84  E-value: 4.56e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLAR-LLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAylpidPDFpverIAYLLSDA------- 587
Cdd:cd05937     5 TWTYSETYDLVLRYAHwLHDDLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAA-----PAF----INYNLSGDplihclk 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  588 --RPVLVVTDaatadrlgPDDITGLvvleetdtggypateppavpaghsayvIYTSGSTGRPKGVVITRAALDNFLAAMA 665
Cdd:cd05937    76 lsGSRFVIVD--------PDDPAIL---------------------------IYTSGTTGLPKAAAISWRRTLVTSNLLS 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  666 ERFPLTAEDRVLATTTVSFDIAG-LELYLPLRSGAGVVLADADTAR--------NPAALIDLAG---------------- 720
Cdd:cd05937   121 HDLNLKNGDRTYTCMPLYHGTAAfLGACNCLMSGGTLALSRKFSASqfwkdvrdSGATIIQYVGelcryllstppspydr 200

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  721 RHRVTLAQAT---PTLWQAL-----VPElsgpalagIRVLVGG-EALPAELARRLGDAGAEVTNMYGPTETTIWSTS--- 788
Cdd:cd05937   201 DHKVRVAWGNglrPDIWERFrerfnVPE--------IGEFYAAtEGVFALTNHNVGDFGAGAIGHHGLIRRWKFENQvvl 272

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  789 ---GPTSEDSIRRGSIGVPIDntqvyvldanlhpAPIG----VLGELHIAGEGLARGYWNRPGLTAEKFLPDPFgPPGTR 861
Cdd:cd05937   273 vkmDPETDDPIRDPKTGFCVR-------------APVGepgeMLGRVPFKNREAFQGYLHNEDATESKLVRDVF-RKGDI 338

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  862 MYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLiNAGPVRRAAAVVREDRP---GDLRLVAYVIPDGPVAP 938
Cdd:cd05937   339 YFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVL-GAHPDIAEANVYGVKVPghdGRAGCAAITLEESSAVP 417

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 653678460  939 DALR----TELSRT-LPDYMIPAVIVPVPDFPTTPNGKLDRAAL 977
Cdd:cd05937   418 TEFTksllASLARKnLPSYAVPLFLRLTEEVATTDNHKQQKGVL 461
PP-binding pfam00550
Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached ...
 997-1049 9.39e-11

Phosphopantetheine attachment site; A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.


Pssm-ID: 425746 [Multi-domain]  Cd Length: 62  Bit Score: 59.11  E-value: 9.39e-11
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 653678460   997 RFLCTAFAEVLGLP--EVAVSDNFFELGGHSLLAFRLLARIRDEFGAGFSLRRLL 1049
Cdd:pfam00550    1 ERLRELLAEVLGVPaeEIDPDTDLFDLGLDSLLAVELIARLEEEFGVEIPPSDLF 55
PLN03102 PLN03102
acyl-activating enzyme; Provisional
 1681-2028 1.65e-10

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 66.20  E-value: 1.65e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1681 DPRGLAYsifTSGSTGQPKGVAVEHIGIvrYLS-------WAAASYPTAGRRGTLAHSSvGFDLTMSalfeplVSGRGVT 1753
Cdd:PLN03102  187 DPISLNY---TSGTTADPKGVVISHRGA--YLStlsaiigWEMGTCPVYLWTLPMFHCN-GWTFTWG------TAARGGT 254

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1754 LMpadatladLAEELSGPLAYDYIRL--------TPSHLRHLV-GHWTGQELPPAARGWVVGGETLDPALVKQLLELrpD 1824
Cdd:PLN03102  255 SV--------CMRHVTAPEIYKNIEMhnvthmccVPTVFNILLkGNSLDLSPRSGPVHVLTGGSPPPAALVKKVQRL--G 324

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1825 AEVINHYGPTETViGRVVH----------PVREAGELAVDSPLplgRPLGETRLQVLDA-WLEAVPVGA--VGELFIGGD 1891
Cdd:PLN03102  325 FQVMHAYGLTEAT-GPVLFcewqdewnrlPENQQMELKARQGV---SILGLADVDVKNKeTQESVPRDGktMGEIVIKGS 400

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1892 GIARGYLGRPALTAEKFlpdpagEPGarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAV 1971
Cdd:PLN03102  401 SIMKGYLKNPKATSEAF------KHG--WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETA 472

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1972 VLVRDQQLVG----WFI----------PAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PLN03102  473 VVAMPHPTWGetpcAFVvlekgettkeDRVDKLVTRERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKI 543
PLN02574 PLN02574
4-coumarate--CoA ligase-like
 1545-2033 2.26e-10

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 66.02  E-value: 2.26e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1545 PGWTALRtgDRSLTFAGLDAQANRLAHALHTGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMAR 1624
Cdd:PLN02574   53 NGDTALI--DSSTGFSISYSELQPLVKSMAAGLYHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGE 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1625 L-TRIADTvTPALVLTRTGQGGCLP--GAEVFGDVPVVALDRVADR-------LTAMPDQRPEVTVDPRGLAYSIFTSGS 1694
Cdd:PLN02574  131 IkKRVVDC-SVGLAFTSPENVEKLSplGVPVIGVPENYDFDSKRIEfpkfyelIKEDFDFVPKPVIKQDDVAAIMYSSGT 209

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEH---IGIVR-YLSWAAASYPTAGRRgtlahssvgfDLTMSALfePLVSGRGVTLMPADatLADLAEELSG 1770
Cdd:PLN02574  210 TGASKGVVLTHrnlIAMVElFVRFEASQYEYPGSD----------NVYLAAL--PMFHIYGLSLFVVG--LLSLGSTIVV 275

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1771 PLAYDYIRLTPSHLRHLVGHWTgqELPP-------AARGwvVGGETLDP-------------ALVKQLLELRPDAEVINH 1830
Cdd:PLN02574  276 MRRFDASDMVKVIDRFKVTHFP--VVPPilmaltkKAKG--VCGEVLKSlkqvscgaaplsgKFIQDFVQTLPHVDFIQG 351

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1831 YGPTE-TVIGrvvhpVR--EAGELAVDSPLPLGRPlgETRLQVLDaWLEA--VPVGAVGELFIGGDGIARGYLGRPALTA 1905
Cdd:PLN02574  352 YGMTEsTAVG-----TRgfNTEKLSKYSSVGLLAP--NMQAKVVD-WSTGclLPPGNCGELWIQGPGVMKGYLNNPKATQ 423

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1906 EKFLPDPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVG---- 1981
Cdd:PLN02574  424 STIDKDG-------WLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGeipv 496

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1982 WFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PLN02574  497 AFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRREL 548
MenH COG0596
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, ...
 2155-2277 2.29e-10

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, alpha/beta hydrolase fold [Coenzyme transport and metabolism, General function prediction only]; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenH and related esterases, alpha/beta hydrolase fold is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440361 [Multi-domain]  Cd Length: 221  Bit Score: 62.71  E-value: 2.29e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2155 DGRPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAE--TIEDMAAgYLADLRLVQPEGPYVVVGWSLGG 2232
Cdd:COG0596    22 DGPPVVLLHGLPGSSYEWRPLIPALAAGYRVIAPDLRGHGRSDKPAGgyTLDDLAD-DLAALLDALGLERVVLVGHSMGG 100

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 653678460 2233 TIAFEMARqldRLGEPVQ-LILLEPTLPEVARTIDLHRPARDSYLR 2277
Cdd:COG0596   101 MVALELAA---RHPERVAgLVLVDEVLAALAEPLRRPGLAPEALAA 143
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
 1552-2011 2.37e-10

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 65.53  E-value: 2.37e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1552 TGDRSLTFAGLDAQANRLAHALHtGALGappVGPETPVLLLLDRSPELVVAMLAVLKAGgyfipvdpgypmarltriadt 1631
Cdd:cd05937     1 FEGKTWTYSETYDLVLRYAHWLH-DDLG---VQAGDFVAIDLTNSPEFVFLWLGLWSIG--------------------- 55

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1632 VTPALVLTRTGqggclpgaevfGDVPVVALDRVADRLtampdqrpeVTVDPRGLAYSIFTSGSTGQPKGVAVehigivry 1711
Cdd:cd05937    56 AAPAFINYNLS-----------GDPLIHCLKLSGSRF---------VIVDPDDPAILIYTSGTTGLPKAAAI-------- 107

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1712 lSWAAaSYPTAGRRGTLAHSSVGfDLTMSALfePLVSGRG-----VTLMPADATLAdLAEELSGPLAYDYIRLTPSHLRH 1786
Cdd:cd05937   108 -SWRR-TLVTSNLLSHDLNLKNG-DRTYTCM--PLYHGTAaflgaCNCLMSGGTLA-LSRKFSASQFWKDVRDSGATIIQ 181

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1787 LVGHwTGQEL---PP-----AARGWVVGGETLDPALVKQLLElRPDAEVINH-YGPTETVIGRVVHPVREAGELAVDSPL 1857
Cdd:cd05937   182 YVGE-LCRYLlstPPspydrDHKVRVAWGNGLRPDIWERFRE-RFNVPEIGEfYAATEGVFALTNHNVGDFGAGAIGHHG 259

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1858 PLGRPLGETRLQVL-------DAWL-------EAVPVGAVGE----LFIGGDGIARGYLGRPALTAEKFLPDpAGEPGAR 1919
Cdd:cd05937   260 LIRRWKFENQVVLVkmdpetdDPIRdpktgfcVRAPVGEPGEmlgrVPFKNREAFQGYLHNEDATESKLVRD-VFRKGDI 338

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1920 MYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqqlVGWFIP-------------- 1985
Cdd:cd05937   339 YFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANV-------YGVKVPghdgragcaaitle 411

                         490       500
                  ....*....|....*....|....*...
gi 653678460 1986 --AEDHPPVTVSALRRFCAEQLPEFMVP 2011
Cdd:cd05937   412 esSAVPTEFTKSLLASLARKNLPSYAVP 439
PLN02246 PLN02246
4-coumarate--CoA ligase
 1552-1709 2.75e-10

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 65.39  E-value: 2.75e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1552 TGdRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADT 1631
Cdd:PLN02246   47 TG-RVYTYADVELLSRRVAAGLH--KLG---IRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKA 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1632 VTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADR-------LTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVE 1704
Cdd:PLN02246  121 SGAKLIITQSCYVDKLKGLAEDDGVTVVTIDDPPEGclhfselTQADENELPEVEISPDDVVALPYSSGTTGLPKGVMLT 200


                  ....*
gi 653678460 1705 HIGIV 1709
Cdd:PLN02246  201 HKGLV 205
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
 1556-1972 3.20e-10

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 65.55  E-value: 3.20e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1556 SLTFAGLDAQANRLAHALHTGAlgapPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTVTPA 1635
Cdd:cd17632    67 TITYAELWERVGAVAAAHDPEQ----PVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPR 142

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1636 LV----------LTRTGQGGCLPGAEVFGDVPVV-----ALDRVADRL------------------TAMPDQRPEVTVDP 1682
Cdd:cd17632   143 LLavsaehldlaVEAVLEGGTPPRLVVFDHRPEVdahraALESARERLaavgipvttltliavrgrDLPPAPLFRPEPDD 222

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1683 RGLAYSIFTSGSTGQPKGVAVEHIGIVRY---LSWAAASYPT-------------AGRR---GTLAHSSVGFDLT---MS 1740
Cdd:cd17632   223 DPLALLIYTSGSTGTPKGAMYTERLVATFwlkVSSIQDIRPPasitlnfmpmshiAGRIslyGTLARGGTAYFAAasdMS 302

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1741 ALFEPLVSGR--GVTLMP--ADATLADLAEELSGPLAYDYIRLTpshLRHLVGHWTGQELPPAARGWVVGGETLDPALVK 1816
Cdd:cd17632   303 TLFDDLALVRptELFLVPrvCDMLFQRYQAELDRRSVAGADAET---LAERVKAELRERVLGGRLLAAVCGSAPLSAEMK 379

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1817 QLLELRPDAEVINHYGPTET----VIGRVVHP---------VREAGELAVDSPLPLGrplgetrlqvldawleavpvgav 1883
Cdd:cd17632   380 AFMESLLDLDLHDGYGSTEAgaviLDGVIVRPpvldyklvdVPELGYFRTDRPHPRG----------------------- 436

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1884 gELFIGGDGIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKL-RGYRIELGEIEARMA 1962
Cdd:cd17632   437 -ELLVKTDTLFPGYYKRPEVTAEVF--DEDG-----FYRTGDVMAELGPDRLVYVDRRNNVLKLsQGEFVTVARLEAVFA 508

                         490
                  ....*....|
gi 653678460 1963 EHPGVEQAVV 1972
Cdd:cd17632   509 ASPLVRQIFV 518
AcpP COG0236
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ...
 2049-2116 3.65e-10

Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440006 [Multi-domain]  Cd Length: 80  Bit Score: 58.33  E-value: 3.65e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460 2049 APRTPGEQLLAELWSTVLG--TDRIGIEDNFF-DLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVRGQA 2116
Cdd:COG0236     1 MPREELEERLAEIIAEVLGvdPEEITPDDSFFeDLGLDSLDAVELIAALEEEfGIELPDTELFEYPTVADLA 72
PLN02479 PLN02479
acetate-CoA ligase
 505-979 3.95e-10

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 65.25  E-value: 3.95e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  505 PGAVAVIEGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL 584
Cdd:PLN02479   34 PTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLL 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  585 SDAR-------------------------------PVLVVTDAATADRLGPDDI--TGLVVLEETDTGGYP--ATEPPAv 629
Cdd:PLN02479  114 EHSKsevvmvdqefftlaeealkilaekkkssfkpPLLIVIGDPTCDPKSLQYAlgKGAIEYEKFLETGDPefAWKPPA- 192

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  630 PAGHSAYVIYTSGSTGRPKGVVIT-RAAldnFLAAMAErfPLT---AEDRVLATTTVSFDIAG--LELYLPLRSGAGVVL 703
Cdd:PLN02479  193 DEWQSIALGYTSGTTASPKGVVLHhRGA---YLMALSN--ALIwgmNEGAVYLWTLPMFHCNGwcFTWTLAALCGTNICL 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  704 ADADTArnpaALIDLAGRHRVTLAQATPTLWQALV----PELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNMYGP 779
Cdd:PLN02479  268 RQVTAK----AIYSAIANYGVTHFCAAPVVLNTIVnapkSETILPLPRVVHVMTAGAAPPPSVLFAMSEKGFRVTHTYGL 343

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  780 TETTIWST---------SGPTSEDS---IRRGSIGVPIDNTQVyVLDANLHPAPI--GVLGELHIAGEGLARGYWNRPGL 845
Cdd:PLN02479  344 SETYGPSTvcawkpewdSLPPEEQArlnARQGVRYIGLEGLDV-VDTKTMKPVPAdgKTMGEIVMRGNMVMKGYLKNPKA 422

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  846 TAEKFlpdpfgppGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLR 925
Cdd:PLN02479  423 NEEAF--------ANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGES 494

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 653678460  926 LVAYVIPDGPV--------APDALRTELSRtLPDYMIPAVIVPVPdFPTTPNGKLDRAALPA 979
Cdd:PLN02479  495 PCAFVTLKPGVdksdeaalAEDIMKFCRER-LPAYWVPKSVVFGP-LPKTATGKIQKHVLRA 554
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
 1549-2036 4.08e-10

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 65.10  E-value: 4.08e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1549 ALRTGDRSLTFAGLDAQANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRI 1628
Cdd:PRK12406    4 TIISGDRRRSFDELAQRAARAA-----GGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1629 -ADTVTPALV----LTRTGQGGCLPGAEVFgDVP----VVALDRVADRLTAMP-----------DQRPEVTVDPRGLAYS 1688
Cdd:PRK12406   79 lEDSGARVLIahadLLHGLASALPAGVTVL-SVPtppeIAAAYRISPALLTPPagaidwegwlaQQEPYDGPPVPQPQSM 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1689 IFTSGSTGQPKGV--------AVEHIGIVRYLSWAAASYPTAGRRGTLAHSSV-GFDLTMSALFEPLVsgrgvtLMP--- 1756
Cdd:PRK12406  158 IYTSGTTGHPKGVrraaptpeQAAAAEQMRALIYGLKPGIRALLTGPLYHSAPnAYGLRAGRLGGVLV------LQPrfd 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1757 ADATLADLAEElsgplAYDYIRLTPSHLRHLVghwtgqELPPAARG--------WVVGGETLDPALVKQLLELRPDAEVI 1828
Cdd:PRK12406  232 PEELLQLIERH-----RITHMHMVPTMFIRLL------KLPEEVRAkydvsslrHVIHAAAPCPADVKRAMIEWWGPVIY 300

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1829 NHYGPTETviGRVVHPVREAgelAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIAR-GYLGRPALTAEK 1907
Cdd:PRK12406  301 EYYGSTES--GAVTFATSED---ALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRAEI 375

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1908 flpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQLvGWFIPA 1986
Cdd:PRK12406  376 ---DRGG-----FITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFgIPDAEF-GEALMA 446

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1987 --EDHPPVTVSA--LRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGP 2036
Cdd:PRK12406  447 vvEPQPGATLDEadIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDP 500
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
 1682-1982 7.98e-10

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 64.37  E-value: 7.98e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1682 PRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPTAGRRGT------LAH--------------SSVGFD--LTM 1739
Cdd:PLN02387  249 PNDIAVIMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVPKLGKNDVylaylpLAHilelaaesvmaavgAAIGYGspLTL 328

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1740 SALFEPL-------VSGRGVTLMPADATLADLAE-------ELSGPLA---YD--YIRltpsHLRHLVGHWTGqelppaA 1800
Cdd:PLN02387  329 TDTSNKIkkgtkgdASALKPTLMTAVPAILDRVRdgvrkkvDAKGGLAkklFDiaYKR----RLAAIEGSWFG------A 398

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1801 RG-----W----------VVGGEtldpalVKQLLE----LRPDAE-VIN---------HYGPTETV------------IG 1839
Cdd:PLN02387  399 WGlekllWdalvfkkiraVLGGR------IRFMLSggapLSGDTQrFINiclgapigqGYGLTETCagatfsewddtsVG 472

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1840 RVVHPV----------REAGELAVDSPLPLGrplgetrlqvldawleavpvgavgELFIGGDGIARGYLGRPALTAEKFL 1909
Cdd:PLN02387  473 RVGPPLpccyvklvswEEGGYLISDKPMPRG------------------------EIVIGGPSVTLGYFKNQEKTDEVYK 528

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1910 PDpagEPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLR-GYRIELGEIEARMAEHPGVEQ-------------AVVLVR 1975
Cdd:PLN02387  529 VD---ERGMRWFYTGDIGQFHPDGCLEIIDRKKDIVKLQhGEYVSLGKVEAALSVSPYVDNimvhadpfhsycvALVVPS 605


                  ....*..
gi 653678460 1976 DQQLVGW 1982
Cdd:PLN02387  606 QQALEKW 612
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
 826-978 8.05e-10

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 63.86  E-value: 8.05e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  826 GELHIAGEGLARGYWnrpgltaekflPDPFGPPgtRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLIN 905
Cdd:PRK07445  302 GNITIQAQSLALGYY-----------PQILDSQ--GIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILA 368

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  906 AGPVRRAAAVVREDRPGDLRLVAYVIP-DGPVAPDALRTELSRTLPDYMIPAVIVPVPDFPTTPNGKLDRAALP 978
Cdd:PRK07445  369 TGLVQDVCVLGLPDPHWGEVVTAIYVPkDPSISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQ 442
PRK03584 PRK03584
acetoacetate--CoA ligase;
503-972 8.57e-10

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 64.05  E-value: 8.57e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  503 RTPGAVAVI-----EGDTTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDF-- 575
Cdd:PRK03584   96 RRDDRPAIIfrgedGPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFgv 175

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  576 -----------P---------------------VERIAYLLSDARPVLVVTDAATADRLGPDDitgLVVLEETDTGGYPA 623
Cdd:PRK03584  176 qgvldrfgqiePkvliavdgyryggkafdrrakVAELRAALPSLEHVVVVPYLGPAAAAAALP---GALLWEDFLAPAEA 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  624 TEPP--AVPAGHSAYVIYTSGSTGRPKGVVITRA-ALDNFLAAMAERFPLTAEDRVLATTTVS-----FDIAGLElylpl 695
Cdd:PRK03584  253 AELEfePVPFDHPLWILYSSGTTGLPKCIVHGHGgILLEHLKELGLHCDLGPGDRFFWYTTCGwmmwnWLVSGLL----- 327

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  696 rSGAGVVLAD-ADTARNPAALIDLAGRHRVTLAQATPTLWQA-----LVPELSGpALAGIR-VLVGGEALPAELARRLGD 768
Cdd:PRK03584  328 -VGATLVLYDgSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDAcekagLVPGETH-DLSALRtIGSTGSPLPPEGFDWVYE 405

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  769 AGAEvtnmygptetTIW--STSG------------PTSedSIRRGSIGVPIDNTQVYVLDANLHPApIGVLGELHIageg 834
Cdd:PRK03584  406 HVKA----------DVWlaSISGgtdicscfvggnPLL--PVYRGEIQCRGLGMAVEAWDEDGRPV-VGEVGELVC---- 468

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  835 lAR-------GYWNRPG---LTAEKFlpDPFgpPGTrmYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIeTTLI 904
Cdd:PRK03584  469 -TKpfpsmplGFWNDPDgsrYRDAYF--DTF--PGV--WRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEI-YRQV 540

                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  905 NAGP-VRRAAAVVREDRPGDLRLVAYVIP-DGPVAPDALRTELSRTLPDYM----IPAVIVPVPDFPTTPNGKL 972
Cdd:PRK03584  541 EALPeVLDSLVIGQEWPDGDVRMPLFVVLaEGVTLDDALRARIRTTIRTNLsprhVPDKIIAVPDIPRTLSGKK 614
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
 584-904 1.03e-09

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 63.98  E-value: 1.03e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  584 LSDARPVLVVTDAATADRLGPDDITGLVV--LEETDTGGYPATEPPAVPAGHSAYVI-YTSGSTGRPKGVVITRAAL--- 657
Cdd:PLN02387  199 LETVKRVIYMDDEGVDSDSSLSGSSNWTVssFSEVEKLGKENPVDPDLPSPNDIAVImYTSGSTGLPKGVMMTHGNIvat 278

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  658 --------------DNFLA--AMAERFPLTAEDRVLAT------------TTVSFDIA------------GLELYLP--- 694
Cdd:PLN02387  279 vagvmtvvpklgknDVYLAylPLAHILELAAESVMAAVgaaigygspltlTDTSNKIKkgtkgdasalkpTLMTAVPail 358

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  695 --LRSGagvVLADADTARNPA-ALIDLAGRHRVTLAQAT--------PTLWQALVPELSGPALAG-IR-VLVGGEALPAE 761
Cdd:PLN02387  359 drVRDG---VRKKVDAKGGLAkKLFDIAYKRRLAAIEGSwfgawgleKLLWDALVFKKIRAVLGGrIRfMLSGGAPLSGD 435

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  762 LARRLGDA-GAEVTNMYGPTETTiwstSGPT-SE-DSIRRGSIGVPIDNTQVYVLD-------ANLHPAPigvLGELHIA 831
Cdd:PLN02387  436 TQRFINIClGAPIGQGYGLTETC----AGATfSEwDDTSVGRVGPPLPCCYVKLVSweeggylISDKPMP---RGEIVIG 508

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 653678460  832 GEGLARGYWNRPGLTAEKFLPDPfgpPGTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLR-GFRIELGEIETTLI 904
Cdd:PLN02387  509 GPSVTLGYFKNQEKTDEVYKVDE---RGMRWFYTGDIGQFHPDGCLEIIDRKKDIVKLQhGEYVSLGKVEAALS 579
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
 1860-2029 1.04e-09

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 62.70  E-value: 1.04e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1860 GRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLDFVG 1939
Cdd:cd17636   166 GRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT--------RGGWHHTNDLGRREPDGSLSFVG 237

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1940 RVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQ---QLVGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWV 2015
Cdd:cd17636   238 PKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIgVPDPrwaQSVKAIVVLKPGASVTEAELIEHCRARIASYKKPKSVE 317

                         170
                  ....*....|....
gi 653678460 2016 ALDAFPLTPHGKVN 2029
Cdd:cd17636   318 FADALPRTAGGADD 331
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
 1554-2033 1.04e-09

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 63.77  E-value: 1.04e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1554 DRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV--------------DPG 1619
Cdd:PRK08276    9 GEVVTYGELEARSNRLAHGLR--ALG---LREGDVVAILLENNPEFFEVYWAARRSGLYYTPInwhltaaeiayivdDSG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1620 ypmARL----TRIADTVTPALVLTRTG------QGGCLPGAEVFGDvpvvALDRVADrlTAMPDQRPEVTvdprgLAYSi 1689
Cdd:PRK08276   84 ---AKVlivsAALADTAAELAAELPAGvplllvVAGPVPGFRSYEE----ALAAQPD--TPIADETAGAD-----MLYS- 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1690 ftSGSTGQPKGV--AVEHIGI-----VRYLSWAAASYPTAGRR----GTLAHSSVG-FDLTMSALFEPLVsgrgvtLMP- 1756
Cdd:PRK08276  149 --SGTTGRPKGIkrPLPGLDPdeapgMMLALLGFGMYGGPDSVylspAPLYHTAPLrFGMSALALGGTVV------VMEk 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1757 --ADATLAdlaeelsgplAYDYIRLTPSHL------RHLvghwtgqELPPAARG--------WVVGGETLDPALVK-QLL 1819
Cdd:PRK08276  221 fdAEEALA----------LIERYRVTHSQLvptmfvRML-------KLPEEVRArydvsslrVAIHAAAPCPVEVKrAMI 283

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1820 ELRPDAeVINHYGPTE----TVIGrvvhpvreaGELAVDSPLPLGRPLgETRLQVLDAWLEAVPVGAVGELFIGGDGIAR 1895
Cdd:PRK08276  284 DWWGPI-IHEYYASSEgggvTVIT---------SEDWLAHPGSVGKAV-LGEVRILDEDGNELPPGEIGTVYFEMDGYPF 352

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1896 GYLGRPALTAEKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-V 1974
Cdd:PRK08276  353 EYHNDPEKTAAARNPH-------GWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFgV 425

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460 1975 RD----QQLVGWFIPAEDH--PPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK08276  426 PDeemgERVKAVVQPADGAdaGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490
PRK07529 PRK07529
AMP-binding domain protein; Validated
 1531-2033 1.07e-09

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 63.82  E-value: 1.07e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1531 RTVHQLVEAQADRTPGWTALR---TGDR-----SLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVA 1602
Cdd:PRK07529   25 ASTYELLSRAAARHPDAPALSfllDADPldrpeTWTYAELLADVTRTANLLH--SLG---VGPGDVVAFLLPNLPETHFA 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1603 MLAVLKAGGYFiPVDP---GYPMARL-----------------TRIADTVTPAL-------VLTRTGQGGCLPGAEVFGD 1655
Cdd:PRK07529  100 LWGGEAAGIAN-PINPllePEQIAELlraagakvlvtlgpfpgTDIWQKVAEVLaalpelrTVVEVDLARYLPGPKRLAV 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1656 VPVV--ALDRVADRLTAMPDQRPEVTVDPRG-----LAYSIFTSGSTGQPKGVAVEHIGIVrYLSWAAAsyptagrrgTL 1728
Cdd:PRK07529  179 PLIRrkAHARILDFDAELARQPGDRLFSGRPigpddVAAYFHTGGTTGMPKLAQHTHGNEV-ANAWLGA---------LL 248

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1729 AHSSVGfDLTMSALfePL--VSGRGVTLMPADATLADLAeeLSGPLAYDYIRLTpSHLRHLVGHW-------------TG 1793
Cdd:PRK07529  249 LGLGPG-DTVFCGL--PLfhVNALLVTGLAPLARGAHVV--LATPQGYRGPGVI-ANFWKIVERYrinflsgvptvyaAL 322

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1794 QELPPAA------RGWVVGGETLdPALVKQLLELRPDAEVINHYGPTETVIGRVVHPVReaGELAVDSplpLGRPLGETR 1867
Cdd:PRK07529  323 LQVPVDGhdisslRYALCGAAPL-PVEVFRRFEAATGVRIVEGYGLTEATCVSSVNPPD--GERRIGS---VGLRLPYQR 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1868 LQVL-----DAWLEAVPVGAVGELFIGGDGIARGYLgrpalTAEKflpDPAGEPGARMYRTGDLVRWRGDGLLDFVGRVD 1942
Cdd:PRK07529  397 VRVVilddaGRYLRDCAVDEVGVLCIAGPNVFSGYL-----EAAH---NKGLWLEDGWLNTGDLGRIDADGYFWLTGRAK 468

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1943 DQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVG----WFIPAEDHPPVTVSALRRFCAEQLPE-FMVPNQWVAL 2017
Cdd:PRK07529  469 DLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGelpvAYVQLKPGASATEAELLAFARDHIAErAAVPKHVRIL 548

                         570
                  ....*....|....*.
gi 653678460 2018 DAFPLTPHGKVNHRAL 2033
Cdd:PRK07529  549 DALPKTAVGKIFKPAL 564
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
 637-973 1.42e-09

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 62.32  E-value: 1.42e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  637 VIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVsFDIAGLELYLP--LRSGAGVVLADADtarnPAA 714
Cdd:cd17636     5 AIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPL-FHIGTLMFTLAtfHAGGTNVFVRRVD----AEE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  715 LIDLAGRHRVTLAQATPTLWQALVpELSGPALAGIRVLVGGEALPAELARRLGDAGAEVTNM--YGPTETT-IWSTSGPT 791
Cdd:cd17636    80 VLELIEAERCTHAFLLPPTIDQIV-ELNADGLYDLSSLRSSPAAPEWNDMATVDTSPWGRKPggYGQTEVMgLATFAALG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  792 SEDSirrGSIGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKFlpdpfgppGTRMYRTGDLVRW 871
Cdd:cd17636   159 GGAI---GGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT--------RGGWHHTNDLGRR 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  872 SAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVI--PDGPVAPDALRTELSRTL 949
Cdd:cd17636   228 EPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVlkPGASVTEAELIEHCRARI 307

                         330       340
                  ....*....|....*....|....
gi 653678460  950 PDYMIPAVIVPVPDFPTTPNGKLD 973
Cdd:cd17636   308 ASYKKPKSVEFADALPRTAGGADD 331
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
 1554-2045 1.71e-09

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 62.96  E-value: 1.71e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1554 DRSLTFAGLDAQANRLAHALHtgALGappVGPETPVLLLLDRSPELVVAMLAVLKAG-------GYF------------- 1613
Cdd:cd05966    82 SRTITYRELLREVCRFANVLK--SLG---VKKGDRVAIYMPMIPELVIAMLACARIGavhsvvfAGFsaesladrindaq 156

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1614 ----IPVDPGYPMAR---LTRIAD-------TVTPALVLTRTGqggclpgaevfGDVPVVA-LDRVADRLtaMPDQRPEV 1678
Cdd:cd05966   157 cklvITADGGYRGGKvipLKEIVDealekcpSVEKVLVVKRTG-----------GEVPMTEgRDLWWHDL--MAKQSPEC 223

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 T---VDPRGLAYSIFTSGSTGQPKGVAVEHIGivrYLSWAAASYPTAgrrgtlahssvgFDLTMSALF------------ 1743
Cdd:cd05966   224 EpewMDSEDPLFILYTSGSTGKPKGVVHTTGG---YLLYAATTFKYV------------FDYHPDDIYwctadigwitgh 288

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1744 -----EPLVSG------RGVTLMPADATLADLAEEL------SGPLAydyIRL------------TPSHLRHLvgHWTGQ 1794
Cdd:cd05966   289 syivyGPLANGattvmfEGTPTYPDPGRYWDIVEKHkvtifyTAPTA---IRAlmkfgdewvkkhDLSSLRVL--GSVGE 363

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1795 ELPPAARGW---VVGGEtldpalvkqllelrpDAEVINHYGPTET---VIgrvvhpvreagelavdSPLP---------L 1859
Cdd:cd05966   364 PINPEAWMWyyeVIGKE---------------RCPIVDTWWQTETggiMI----------------TPLPgatplkpgsA 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1860 GRPLGETRLQVLDAWLEAVPVGAVGELFIGGD--GIARGYLGRPALTAEKFLPDpagEPGarMYRTGDLVRWRGDGLLDF 1937
Cdd:cd05966   413 TRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPwpGMARTIYGDHERYEDTYFSK---FPG--YYFTGDGARRDEDGYYWI 487

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1938 VGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRD----QQLVGWFIPAEDHPP--VTVSALRRFCAEQLPEFMV 2010
Cdd:cd05966   488 TGRVDDVINVSGHRLGTAEVESALVAHPAVaEAAVVGRPHdikgEAIYAFVTLKDGEEPsdELRKELRKHVRKEIGPIAT 567

                         570       580       590
                  ....*....|....*....|....*....|....*
gi 653678460 2011 PNQWVALDAFPLTPHGKVNHRALPGPTSGRPELED 2045
Cdd:cd05966   568 PDKIQFVPGLPKTRSGKIMRRILRKIAAGEEELGD 602
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
 1541-2040 2.43e-09

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 62.40  E-value: 2.43e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTAL---RTGDrSLTFAGLDAQANRLAHALHTGALgappvGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD 1617
Cdd:PRK13391    7 AQTTPDKPAVimaSTGE-VVTYRELDERSNRLAHLFRSLGL-----KRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVN 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEvfGDVPVVALDRVADRLTAMP---------DQRPEVTVD--PRG-- 1684
Cdd:PRK13391   81 SHLTPAEAAYIVDDSGARALITSAAKLDVARALL--KQCPGVRHRLVLDGDGELEgfvgyaeavAGLPATPIAdeSLGtd 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1685 LAYSiftSGSTGQPKGVavehigivrylswaaasyptagrRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADl 1764
Cdd:PRK13391  159 MLYS---SGTTGRPKGI-----------------------KRPLPEQPPDTPLPLTAFLQRLWGFRSDMVYLSPAPLYH- 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1765 aeelSGPLAYDYI------------RLTPSHLRHLVGHWT---GQ----------ELPPAARG--------WVVGGETLD 1811
Cdd:PRK13391  212 ----SAPQRAVMLvirlggtvivmeHFDAEQYLALIEEYGvthTQlvptmfsrmlKLPEEVRDkydlssleVAIHAAAPC 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1812 PALVKQllelrpdaEVINHYGPtetVIGRVVHPVREAGELAVDSPLPLGRP-------LGEtrLQVLDAWLEAVPVGAVG 1884
Cdd:PRK13391  288 PPQVKE--------QMIDWWGP---IIHEYYAATEGLGFTACDSEEWLAHPgtvgramFGD--LHILDDDGAELPPGEPG 354

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1885 ELFIGGdGIARGYLGRPALTAEKFLPDPAgepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEH 1964
Cdd:PRK13391  355 TIWFEG-GRPFEYLNDPAKTAEARHPDGT------WSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITH 427

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1965 PGVEQAVVL-VRDQQL------VGWFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRALPGPT 2037
Cdd:PRK13391  428 PKVADAAVFgVPNEDLgeevkaVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRDRY 507


                  ...
gi 653678460 2038 SGR 2040
Cdd:PRK13391  508 WGN 510
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
 544-882 3.19e-09

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 62.44  E-value: 3.19e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  544 VAVLLPRSADLLVTLLAVIKAGAAYLPI-DPDFP--VERIAYLLSDARP--VLVVTDAATADR-----LGPDDITGLVVL 613
Cdd:PRK07769   82 VAILAPQNLDYLIAFFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCTPsaILTTTDSAEGVRkffraRPAKERPRVIAV 161

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  614 EETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATTTVSFDIAGLELYL 693
Cdd:PRK07769  162 DAVPDEVGATWVPPEANEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVSWLPFFHDMGLITVLL 241

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  694 PLRSGAGVvladadTARNPAALIDLAGRHRVTLAQA---TPTLWQAL------------VPELSGPAL-----AGIrvLV 753
Cdd:PRK07769  242 PALLGHYI------TFMSPAAFVRRPGRWIRELARKpggTGGTFSAApnfafehaaargLPKDGEPPLdlsnvKGL--LN 313

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  754 GGEALPAELARRLGDAGA-------EVTNMYGPTETTIWSTSGP-------------------------TSEDSIRRGSI 801
Cdd:PRK07769  314 GSEPVSPASMRKFNEAFApyglpptAIKPSYGMAEATLFVSTTPmdeeptviyvdrdelnagrfvevpaDAPNAVAQVSA 393

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  802 G-VPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARGYWNRPGLTAEKF-------LPDPFG---PPGTRMYRTGDLVR 870
Cdd:PRK07769  394 GkVGVSEWAVIVDPETASELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqnilksrLSESHAegaPDDALWVRTGDYGV 473

                         410
                  ....*....|..
gi 653678460  871 WsAAGDLEYLGR 882
Cdd:PRK07769  474 Y-FDGELYITGR 484
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
1827-2033 3.28e-09

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 62.20  E-value: 3.28e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1827 VINHYGPTETVIGRVVHPVReagelAVDSPLPLGRPLGETRLQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAE 1906
Cdd:PRK08751  357 LVEAYGLTETSPAACINPLT-----LKEYNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAK 431

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1907 KFlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVVLVRDQQ---LVGW 1982
Cdd:PRK08751  432 VM--DADG-----WLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVlEVAAVGVPDEKsgeIVKV 504

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1983 FIPAEDhPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK08751  505 VIVKKD-PALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
PRK09192 PRK09192
fatty acyl-AMP ligase;
517-976 5.43e-09

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 61.56  E-value: 5.43e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  517 LSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAG--AAYLPIDPDFP-----VERIAYLLSDARP 589
Cdd:PRK09192   50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGlvPVPLPLPMGFGgresyIAQLRGMLASAQP 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  590 VLVV--------TDAATADRLGPDDITGlvvlEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFL 661
Cdd:PRK09192  130 AAIItpdellpwVNEATHGNPLLHVLSH----AWFKALPEADVALPRPTPDDIAYLQYSSGSTRFPRGVIITHRALMANL 205

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  662 AAMAER-FPLTAEDR----------------VLA--TTTVSFDiaglelYLPLRsgagvvladaDTARNPAALIDLAGRH 722
Cdd:PRK09192  206 RAISHDgLKVRPGDRcvswlpfyhdmglvgfLLTpvATQLSVD------YLPTR----------DFARRPLQWLDLISRN 269

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  723 RVTLAQAtPTLW----------QALVP-ELSGPALAGIrvlvGGEALPAELARRLGDAGAEV-------TNMYGPTETTI 784
Cdd:PRK09192  270 RGTISYS-PPFGyelcarrvnsKDLAElDLSCWRVAGI----GADMIRPDVLHQFAEAFAPAgfddkafMPSYGLAEATL 344

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  785 WSTSGP----------------------TSEDSIRRGS----IGVPIDNTQVYVLDANLHPAPIGVLGELHIAGEGLARG 838
Cdd:PRK09192  345 AVSFSPlgsgivveevdrdrleyqgkavAPGAETRRVRtfvnCGKALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSG 424

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  839 YWNRPgLTAEKFLPDPFgppgtrmYRTGDLvRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVR-RAAAVVR 917
Cdd:PRK09192  425 YFRDE-ESQDVLAADGW-------LDTGDL-GYLLDGYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELRsGDAAAFS 495

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460  918 EDRPGDLRLVAYV---IPDgPVAPDALRTELSRTLpdYMIPAV-----IVPVPDFPTTPNGKLDRAA 976
Cdd:PRK09192  496 IAQENGEKIVLLVqcrISD-EERRGQLIHALAALV--RSEFGVeaaveLVPPHSLPRTSSGKLSRAK 559
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
 1691-2033 7.35e-09

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 60.19  E-value: 7.35e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1691 TSGSTGQPKGVAVEHIGIVrYLSWAAASYPTAGRRGTLahssvgfdltMSALfePL--VSGRGVTLMPADATLADLAeeL 1768
Cdd:cd05944    10 TGGTTGTPKLAQHTHSNEV-YNAWMLALNSLFDPDDVL----------LCGL--PLfhVNGSVVTLLTPLASGAHVV--L 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1769 SGPLAYDYIRLTPSHLRhLVGHWTGQELP--PAA----------------RGWVVGGETLDPALVKQLlELRPDAEVINH 1830
Cdd:cd05944    75 AGPAGYRNPGLFDNFWK-LVERYRITSLStvPTVyaallqvpvnadisslRFAMSGAAPLPVELRARF-EDATGLPVVEG 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1831 YGPTETVIGRVVHPVREAGEL-AVDSPLPLGRplgeTRLQVLDA---WLEAVPVGAVGELFIGGDGIARGYL----GRPA 1902
Cdd:cd05944   153 YGLTEATCLVAVNPPDGPKRPgSVGLRLPYAR----VRIKVLDGvgrLLRDCAPDEVGEICVAGPGVFGGYLytegNKNA 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1903 LTAEKFLpdpagepgarmyRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVG- 1981
Cdd:cd05944   229 FVADGWL------------NTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGe 296

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1982 ---WFIPAEDHPPVTVSALRRFCAEQLPE-FMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05944   297 lpvAYVQLKPGAVVEEEELLAWARDHVPErAAVPKHIEVLEELPVTAVGKVFKPAL 352
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
 635-904 8.35e-09

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 60.60  E-value: 8.35e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  635 AYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLATT----TVSFDIAGLelyLPLRSGAGVVLadADTAR 710
Cdd:PRK06334  186 AVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLppfhAYGFNSCTL---FPLLSGVPVVF--AYNPL 260

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  711 NPAALIDLAGRHRVTLAQATPTLWQALV--PELSGPALAGIR-VLVGGEALPAELARRLGDAGAEVT--NMYGPTETTIW 785
Cdd:PRK06334  261 YPKKIVEMIDEAKVTFLGSTPVFFDYILktAKKQESCLPSLRfVVIGGDAFKDSLYQEALKTFPHIQlrQGYGTTECSPV 340

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  786 STSgpTSEDSIRRGS-IGVPIDNTQVYVLDANLH-PAPIGVLGELHIAGEGLARGYW-NRPGltaEKFLpdPFGppGTRM 862
Cdd:PRK06334  341 ITI--NTVNSPKHEScVGMPIRGMDVLIVSEETKvPVSSGETGLVLTRGTSLFSGYLgEDFG---QGFV--ELG--GETW 411

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|..
gi 653678460  863 YRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLI 904
Cdd:PRK06334  412 YVTGDLGYVDRHGELFLKGRLSRFVKIGAEMVSLEALESILM 453
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
 1956-2027 1.06e-08

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 54.09  E-value: 1.06e-08
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460  1956 EIEARMAEHPGVEQ-AVVLVRD----QQLVGwFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGK 2027
Cdd:pfam13193    1 EVESALVSHPAVAEaAVVGVPDelkgEAPVA-FVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
 1859-2034 1.72e-08

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 59.62  E-value: 1.72e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1859 LGRPLGETRLQVLDawleavpvGAVGELFIGGDGIARGYLgrpaltaekflpdPAGEPGARMYRTGDLVRWRGDGLLDFV 1938
Cdd:PRK07445  285 SGQVLPHAQITIPA--------NQTGNITIQAQSLALGYY-------------PQILDSQGIFETDDLGYLDAQGYLHIL 343

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1939 GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRD----QQLVGWFIPAedHPPVTVSALRRFCAEQLPEFMVPNQ 2013
Cdd:PRK07445  344 GRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLgLPDphwgEVVTAIYVPK--DPSISLEELKTAIKDQLSPFKQPKH 421

                         170       180
                  ....*....|....*....|.
gi 653678460 2014 WVALDAFPLTPHGKVNHRALP 2034
Cdd:PRK07445  422 WIPVPQLPRNPQGKINRQQLQ 442
AcpP COG0236
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ...
 991-1048 1.82e-08

Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440006 [Multi-domain]  Cd Length: 80  Bit Score: 53.32  E-value: 1.82e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460  991 PRDDRERFLCTAFAEVLGLP--EVAVSDNFF-ELGGHSLLAFRLLARIRDEFGAGFSLRRL 1048
Cdd:COG0236     2 PREELEERLAEIIAEVLGVDpeEITPDDSFFeDLGLDSLDAVELIAALEEEFGIELPDTEL 62
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
 1538-2033 2.09e-08

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 59.31  E-value: 2.09e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1538 EAQADRTPGWTALRtgdRSLTFAGLDAQANRLAHALHTGalgapPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVD 1617
Cdd:cd05929     2 EARDLDRAQVFHQR---RLLLLDVYSIALNRNARAAAAE-----GVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKS 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1618 PGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVFgdvpvvaldrvadrlTAMPDQRPEVTVDPRGLAYSIFTS-GSTG 1696
Cdd:cd05929    74 SRAPRAEACAIIEIKAAALVCGLFTGGGALDGLEDY---------------EAAEGGSPETPIEDEAAGWKMLYSgGTTG 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1697 QPKGVAVEH----IGIVRYLSWAAASYPTAGRR----GTLAHSSvGFDLTMSALFeplvSGRGVTLMP---ADATLADLA 1765
Cdd:cd05929   139 RPKGIKRGLpggpPDNDTLMAAALGFGPGADSVylspAPLYHAA-PFRWSMTALF----MGGTLVLMEkfdPEEFLRLIE 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1766 EElsgplAYDYIRLTPSHLRHLVghwtgqELPPAARG----------WVVGGETldPALVKQ-LLELRPDAeVINHYGPT 1834
Cdd:cd05929   214 RY-----RVTFAQFVPTMFVRLL------KLPEAVRNaydlsslkrvIHAAAPC--PPWVKEqWIDWGGPI-IWEYYGGT 279

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1835 E----TVIgrvvhpvreAGELAVDSPLPLGRPLGeTRLQVLDAWLEAVPVGAVGEL-FIGGDGIArgYLGRPALTAEKFl 1909
Cdd:cd05929   280 EgqglTII---------NGEEWLTHPGSVGRAVL-GKVHILDEDGNEVPPGEIGEVyFANGPGFE--YTNDPEKTAAAR- 346

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1910 pdpagepGARMYRT-GDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVL-VRDQQL------VG 1981
Cdd:cd05929   347 -------NEGGWSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVgVPDEELgqrvhaVV 419

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 653678460 1982 WFIPAEDHPPVTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:cd05929   420 QPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLL 471
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
 1553-1980 2.56e-08

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 58.99  E-value: 2.56e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1553 GDRSLTFAGLDAQANRLAHALHTGalgapPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARLTRIADTV 1632
Cdd:cd05914     4 GGEPLTYKDLADNIAKFALLLKIN-----GVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1633 TPALVLTRtgqggclpgaevfgdvpvvaldrvadrltampdqrpevtvDPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYL 1712
Cdd:cd05914    79 EAKAIFVS----------------------------------------DEDDVALINYTSGTTGNSKGVMLTYRNIVSNV 118

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1713 SWAAASYPTAGRRGTLA----HSSVGFDLTMsalFEPLVSGRGVTLM--PADATLADLAEE-------LSGPLAYDYIRL 1779
Cdd:cd05914   119 DGVKEVVLLGKGDKILSilplHHIYPLTFTL---LLPLLNGAHVVFLdkIPSAKIIALAFAqvtptlgVPVPLVIEKIFK 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1780 T---PS-----------------HLRHLVGHWTGQELPPAARGWVVGGETLDPALVKQLLELrpDAEVINHYGPTETvig 1839
Cdd:cd05914   196 MdiiPKltlkkfkfklakkinnrKIRKLAFKKVHEAFGGNIKEFVIGGAKINPDVEEFLRTI--GFPYTIGYGMTET--- 270

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1840 rvvhpvreagelavdSPLPLGRPLGETRL----QVLDAwLEA-----VPVGAVGELFIGGDGIARGYLGRPALTAEKFLP 1910
Cdd:cd05914   271 ---------------APIISYSPPNRIRLgsagKVIDG-VEVridspDPATGEGEIIVRGPNVMKGYYKNPEATAEAFDK 334

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1911 DPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKL-RGYRIELGEIEARMAEHPGVEQAVVLVRDQQLV 1980
Cdd:cd05914   335 DG-------WFHTGDLGKIDAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLV 398
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
 1541-2028 4.22e-08

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 58.48  E-value: 4.22e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1541 ADRTPGWTALRTGDrSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGY 1620
Cdd:PRK13390   10 APDRPAVIVAETGE-QVSYRQLDDDSAALARVLYDAGLRTGDV-----VALLSDNSPEALVVLWAALRSGLYITAINHHL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1621 PMARLTRI-ADTVTPALVLTRTGQGgclPGAEVFGDVPVV-----ALDRVADRLTAMPDQRPEVTVDPRGlAYSIFTSGS 1694
Cdd:PRK13390   84 TAPEADYIvGDSGARVLVASAALDG---LAAKVGADLPLRlsfggEIDGFGSFEAALAGAGPRLTEQPCG-AVMLYSSGT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1695 TGQPKGVAVEHIGivryLSWAAASYPTAGRRGTLahssvgFDLTMSALF---EPLVSG---RGVTLMPADATLADLAEEL 1768
Cdd:PRK13390  160 TGFPKGIQPDLPG----RDVDAPGDPIVAIARAF------YDISESDIYyssAPIYHAaplRWCSMVHALGGTVVLAKRF 229

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1769 SGPLAYDYI---RLTPSH--------LRHLVGHWTGQELPPAARGwVVGGETLDPALVKQLLELRPDAEVINHYGPTEtv 1837
Cdd:PRK13390  230 DAQATLGHVeryRITVTQmvptmfvrLLKLDADVRTRYDVSSLRA-VIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTE-- 306

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1838 igrvVHpvreaGELAVDSPLPLGRPLGETR-----LQVLDAWLEAVPVGAVGELFIGGDGIARGYLGRPALTAEkflpdp 1912
Cdd:PRK13390  307 ----AH-----GMTFIDSPDWLAHPGSVGRsvlgdLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAA------ 371

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1913 AGEPGARMYRT-GDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQ-AVVLVRDQ----------QLV 1980
Cdd:PRK13390  372 AQHPAHPFWTTvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDvAVIGVPDPemgeqvkaviQLV 451

                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*...
gi 653678460 1981 GWFIPAEDhppvTVSALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKV 2028
Cdd:PRK13390  452 EGIRGSDE----LARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKL 495
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
1654-2033 4.44e-08

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 58.82  E-value: 4.44e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1654 GDVPVVALDRVADRLTAM-------PDQRPEVTV---DPRGLAYSIFTSGSTGQPKGVAVEHIGIVRYLSWAAASYPtAG 1723
Cdd:PRK06814  754 FGIRIIYLEDVRAQIGLAdkikgllAGRFPLVYFcnrDPDDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARID-FS 832

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1724 RRGT------LAHSsvgFDLTMSALFePLVSGRGVTLMP--------------ADATL-----------ADLAEelsgpl 1772
Cdd:PRK06814  833 PEDKvfnalpVFHS---FGLTGGLVL-PLLSGVKVFLYPsplhyriipeliydTNATIlfgtdtflngyARYAH------ 902

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1773 AYDYIRLtpshlrhlvghwtgqelppaaRGWVVGGETLDPALVKQLLE---LRpdaeVINHYGPTETVigrvvhPVreag 1849
Cdd:PRK06814  903 PYDFRSL---------------------RYVFAGAEKVKEETRQTWMEkfgIR----ILEGYGVTETA------PV---- 947

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1850 eLAVDSPL-----PLGR--PLGETRLqvldawlEAVP-VGAVGELFIGGDGIARGYLgrpalTAEKflPDPAGEPGARMY 1921
Cdd:PRK06814  948 -IALNTPMhnkagTVGRllPGIEYRL-------EPVPgIDEGGRLFVRGPNVMLGYL-----RAEN--PGVLEPPADGWY 1012

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1922 RTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAE-HPGVEQAVVLVRDQ----QLVgWFIPAEDhppVTVSA 1996
Cdd:PRK06814 1013 DTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAElWPDALHAAVSIPDArkgeRII-LLTTASD---ATRAA 1088

                         410       420       430
                  ....*....|....*....|....*....|....*...
gi 653678460 1997 LRRFC-AEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK06814 1089 FLAHAkAAGASELMVPAEIITIDEIPLLGTGKIDYVAV 1126
PaaK COG1541
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ...
 628-949 8.26e-08

Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];


Pssm-ID: 441150 [Multi-domain]  Cd Length: 423  Bit Score: 57.08  E-value: 8.26e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  628 AVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERF---PLTAEDRVLATTTVSFDIAGLELYLPL-RSGAGVVL 703
Cdd:COG1541    79 AVPLEEIVRIHASSGTTGKPTVVGYTRKDLDRWAELFARSLraaGVRPGDRVQNAFGYGLFTGGLGLHYGAeRLGATVIP 158

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  704 AdadTARNPAALIDLAGRHRVTLAQATPTlwQAL----VPELSG--PALAGIRV-LVGGEALPAELARRLGDA-GAEVTN 775
Cdd:COG1541   159 A---GGGNTERQLRLMQDFGPTVLVGTPS--YLLylaeVAEEEGidPRDLSLKKgIFGGEPWSEEMRKEIEERwGIKAYD 233

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  776 MYGPTETtiwstsGPT--SEDSIRRGSIgvpIDNTQVYV--LD-ANLHPAPIGVLGEL---HIAGEG--LARgywnrpgl 845
Cdd:COG1541   234 IYGLTEV------GPGvaYECEAQDGLH---IWEDHFLVeiIDpETGEPVPEGEEGELvvtTLTKEAmpLIR-------- 296

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  846 taekflpdpfgppgtrmYRTGDLVRWSAAGD--------LEY-LGRTDHQVKLRGFRIELGEIETTLINAGPVrRAAAVV 916
Cdd:COG1541   297 -----------------YRTGDLTRLLPEPCpcgrthprIGRiLGRADDMLIIRGVNVFPSQIEEVLLRIPEV-GPEYQI 358

                         330       340       350
                  ....*....|....*....|....*....|....
gi 653678460  917 REDRPGDL-RLVAYVIPDGPVAPDALRTELSRTL 949
Cdd:COG1541   359 VVDREGGLdELTVRVELAPGASLEALAEAIAAAL 392
prpE PRK10524
propionyl-CoA synthetase; Provisional
 515-979 1.17e-07

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 57.27  E-value: 1.17e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  515 TTLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVLVVT 594
Cdd:PRK10524   83 RTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDAKPVLIVS 162

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  595 -----------------DAATA------------DR-LGPD-DITGLVVLEETDTGGYPATEPPA--VPAGHSAYVIYTS 641
Cdd:PRK10524  163 adagsrggkvvpykpllDEAIAlaqhkprhvllvDRgLAPMaRVAGRDVDYATLRAQHLGARVPVewLESNEPSYILYTS 242

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  642 GSTGRPKGVVITRAALDNFLAA-MAERFPLTAEDRVLATTTV------SFDIaglelYLPLRSGAGVVLADADTAR-NPA 713
Cdd:PRK10524  243 GTTGKPKGVQRDTGGYAVALATsMDTIFGGKAGETFFCASDIgwvvghSYIV-----YAPLLAGMATIMYEGLPTRpDAG 317

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  714 ALIDLAGRHRVTLAQATPTLWQALvpELSGPA------LAGIRVL-VGGEALPAELARRLGDA-GAEVTNMYGPTETTiW 785
Cdd:PRK10524  318 IWWRIVEKYKVNRMFSAPTAIRVL--KKQDPAllrkhdLSSLRALfLAGEPLDEPTASWISEAlGVPVIDNYWQTETG-W 394

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  786 S--TSGPTSEDS-IRRGSIGVPIDNTQVYVLD-ANLHPAPIGVLGELHIAG---EGLARGYWNrpglTAEKFLPDPFGPP 858
Cdd:PRK10524  395 PilAIARGVEDRpTRLGSPGVPMYGYNVKLLNeVTGEPCGPNEKGVLVIEGplpPGCMQTVWG----DDDRFVKTYWSLF 470

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  859 GTRMYRTGDLVRWSAAGDLEYLGRTDHQVKLRGFRIELGEIETTLINAGPVRRAAAVVREDRPGDLRLVAYVIP------ 932
Cdd:PRK10524  471 GRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPkdsdsl 550

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460  933 DGPVAPDALRTELSRTLPDYM----IPAVIVPVPDFPTTPNGKLDRAALPA 979
Cdd:PRK10524  551 ADREARLALEKEIMALVDSQLgavaRPARVWFVSALPKTRSGKLLRRAIQA 601
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
 898-971 1.17e-07

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 51.01  E-value: 1.17e-07
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460   898 EIETTLINAGPVRRAAAV-VREDRPGDlRLVAYVIPDGPVAP--DALRTELSRTLPDYMIPAVIVPVPDFPTTPNGK 971
Cdd:pfam13193    1 EVESALVSHPAVAEAAVVgVPDELKGE-APVAFVVLKPGVELleEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
 1585-1949 2.34e-07

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 56.28  E-value: 2.34e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1585 PETPVLLLLDRSPELVVAMLAVLKAGGYFIPV-DPGYP--MARLTRIADTVTPALVLTRTGqggclpGAE----VFGDVP 1657
Cdd:PRK07769   78 PGDRVAILAPQNLDYLIAFFGALYAGRIAVPLfDPAEPghVGRLHAVLDDCTPSAILTTTD------SAEgvrkFFRARP 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1658 ------VVALDRVADRLTAMPdQRPEVTVDPrgLAYSIFTSGSTGQPKGVAVEHIGI-VRYLSWAAASYPTAGRRGT--- 1727
Cdd:PRK07769  152 akerprVIAVDAVPDEVGATW-VPPEANEDT--IAYLQYTSGSTRIPAGVQITHLNLpTNVLQVIDALEGQEGDRGVswl 228

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1728 -LAHssvgfDLTMSALFEPLVSGRGVTLMpadatladlaeelsGPLAydYIRLTPSHLRHL--VGHWTGQELP------- 1797
Cdd:PRK07769  229 pFFH-----DMGLITVLLPALLGHYITFM--------------SPAA--FVRRPGRWIRELarKPGGTGGTFSaapnfaf 287

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1798 --PAARGWVVGGE-TLDPALVKQLLE---------LRPDAEVINHYGPTETVI---------------------GRVVHP 1844
Cdd:PRK07769  288 ehAAARGLPKDGEpPLDLSNVKGLLNgsepvspasMRKFNEAFAPYGLPPTAIkpsygmaeatlfvsttpmdeePTVIYV 367

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1845 VREagELA------VDSPLPLGRPLGETRLQVLDAWLEAV--------PVGAVGELFIGGDGIARGYLGRPALTAEKF-- 1908
Cdd:PRK07769  368 DRD--ELNagrfveVPADAPNAVAQVSAGKVGVSEWAVIVdpetaselPDGQIGEIWLHGNNIGTGYWGKPEETAATFqn 445

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*....
gi 653678460 1909 -----LPDPAGE---PGARMYRTGDLVRWRgDGLLDFVGRVDDQVKLRG 1949
Cdd:PRK07769  446 ilksrLSESHAEgapDDALWVRTGDYGVYF-DGELYITGRVKDLVIIDG 493
PLN02479 PLN02479
acetate-CoA ligase
 1861-2033 2.63e-07

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 56.01  E-value: 2.63e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1861 RPLGETRLQVLDAwLEAVPVGA----VGELFIGGDGIARGYLGRPALTAEKFlpdpagepGARMYRTGDLVRWRGDGLLD 1936
Cdd:PLN02479  377 RYIGLEGLDVVDT-KTMKPVPAdgktMGEIVMRGNMVMKGYLKNPKANEEAF--------ANGWFHSGDLGVKHPDGYIE 447

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1937 FVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVR-DQQlvgW------FIPAED-----HPPVTVSALRRFCAEQ 2004
Cdd:PLN02479  448 IKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARpDER---WgespcaFVTLKPgvdksDEAALAEDIMKFCRER 524

                         170       180
                  ....*....|....*....|....*....
gi 653678460 2005 LPEFMVPNQwVALDAFPLTPHGKVNHRAL 2033
Cdd:PLN02479  525 LPAYWVPKS-VVFGPLPKTATGKIQKHVL 552
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
 1689-2033 2.69e-07

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 56.00  E-value: 2.69e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1689 IFTSGSTGQPKGVAVEHIGIVRYLSwaAASYPTAGRR--------------------GTLAHSSVGFDLTMSALFEPLVS 1748
Cdd:cd17642   190 MNSSGSTGLPKGVQLTHKNIVARFS--HARDPIFGNQiipdtailtvipfhhgfgmfTTLGYLICGFRVVLMYKFEEELF 267

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1749 GRGV------------TLMP--ADATLADlaeelsgplAYDYirltpSHLRHLVGhwtgqelppaargwvvGGETLDPAL 1814
Cdd:cd17642   268 LRSLqdykvqsallvpTLFAffAKSTLVD---------KYDL-----SNLHEIAS----------------GGAPLSKEV 317

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1815 VKQLLELRPDAEVINHYGPTETVIGRVVHPVREagelavDSPLPLGR--PLGETRLQVLDAWlEAVPVGAVGELFIGGDG 1892
Cdd:cd17642   318 GEAVAKRFKLPGIRQGYGLTETTSAILITPEGD------DKPGAVGKvvPFFYAKVVDLDTG-KTLGPNERGELCVKGPM 390

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1893 IARGYLGRPALTAEkfLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVV 1972
Cdd:cd17642   391 IMKGYVNNPEATKA--LIDKDG-----WLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGV 463

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1973 L-VRDQ---QLVGWFIPAEDHPPVTVSALRRFCAEQLPefmvPNQW-----VALDAFPLTPHGKVNHRAL 2033
Cdd:cd17642   464 AgIPDEdagELPAAVVVLEAGKTMTEKEVMDYVASQVS----TAKRlrggvKFVDEVPKGLTGKIDRRKI 529
hsFATP2a_ACSVL_like cd05938
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ...
 513-653 4.27e-07

Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341261 [Multi-domain]  Cd Length: 537  Bit Score: 55.38  E-value: 4.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  513 GDTTLSYRELDERANRLAR-LLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLLSDARPVL 591
Cdd:cd05938     2 EGETYTYRDVDRRSNQAARaLLAHAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  592 VVTDAATADRLGP-------DDITGLVVLEETDTGGY-------PATEPPAVPAGHSAYV--------IYTSGSTGRPKG 649
Cdd:cd05938    82 LVVAPELQEAVEEvlpalraDGVSVWYLSHTSNTEGVislldkvDAASDEPVPASLRAHVtikspalyIYTSGTTGLPKA 161


                  ....
gi 653678460  650 VVIT 653
Cdd:cd05938   162 ARIS 165
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
 1533-2033 4.55e-07

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 54.99  E-value: 4.55e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1533 VHQLVEAQADRTPGWTALrTGdRSLTFAGLDAQANRLAHALHTGALGAPPVgpetpVLLLLDRSPELVVAMLAVLKAGGY 1612
Cdd:PLN02330   34 VLQDAELYADKVAFVEAV-TG-KAVTYGEVVRDTRRFAKALRSLGLRKGQV-----VVVVLPNVAEYGIVALGIMAAGGV 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1613 FIPVDPGYPMARLTRIADTVTPALVLTRTGQGGCLPGAEVfgdvPVVALDRV--------------ADRLTAMPDQRPEV 1678
Cdd:PLN02330  107 FSGANPTALESEIKKQAEAAGAKLIVTNDTNYGKVKGLGL----PVIVLGEEkiegavnwkelleaADRAGDTSDNEEIL 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TVDPRGLAysiFTSGSTGQPKGVAVEHIGIVRYL--SWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMP 1756
Cdd:PLN02330  183 QTDLCALP---FSSGTTGISKGVMLTHRNLVANLcsSLFSVGPEMIGQVVTLGLIPFFHIYGITGICCATLRNKGKVVVM 259

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1757 ADATL-----ADLAEELSgplaydYIRLTPSHLRHLVGHWTGQELPPAA---RGWVVGGETLDPALVKQLLELRPDAEVI 1828
Cdd:PLN02330  260 SRFELrtflnALITQEVS------FAPIVPPIILNLVKNPIVEEFDLSKlklQAIMTAAAPLAPELLTAFEAKFPGVQVQ 333

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1829 NHYGPTETVIGRVVHPVREAGE-LAVDSPLPLGRPLGETRLQVLDAWLeAVPVGAVGELFIGGDGIARGYLGRPALTAEK 1907
Cdd:PLN02330  334 EAYGLTEHSCITLTHGDPEKGHgIAKKNSVGFILPNLEVKFIDPDTGR-SLPKNTPGELCVRSQCVMQGYYNNKEETDRT 412

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1908 FlpDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWfIPAe 1987
Cdd:PLN02330  413 I--DEDG-----WLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGE-IPA- 483

                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460 1988 dhPPVTVSALRRFCAEQLPEFMVPN----------QWValDAFPLTPHGKVNHRAL 2033
Cdd:PLN02330  484 --ACVVINPKAKESEEDILNFVAANvahykkvrvvQFV--DSIPKSLSGKIMRRLL 535
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
 1548-2032 5.57e-07

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 55.00  E-value: 5.57e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1548 TALRTGDRSLTFAGLDA-----------QANRLAhalhtGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPV 1616
Cdd:PRK07768   10 ANARTSPRGMVTGEPDApvrhtwgevheRARRIA-----GGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTML 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1617 DPGYPMARLTR-IADTVTpalVLTRTGQGGCLPGAEVFGDVPV-----VALDRVADRLTAMPDQRPEVTVDprGLAYSIF 1690
Cdd:PRK07768   85 HQPTPRTDLAVwAEDTLR---VIGMIGAKAVVVGEPFLAAAPVleekgIRVLTVADLLAADPIDPVETGED--DLALMQL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1691 TSGSTGQPKGVAVEHIGIvrylsWAAASYPTAGrrgtlAHSSVGFDLTMSALfePLVSGRGVT---LMP----ADATLAD 1763
Cdd:PRK07768  160 TSGSTGSPKAVQITHGNL-----YANAEAMFVA-----AEFDVETDVMVSWL--PLFHDMGMVgflTVPmyfgAELVKVT 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1764 LAEELSGPLAY----DYIRLT----PSHLRHLVGHWTGQELPPAA------RGWVVGGETLDPALVKQLLE------LRP 1823
Cdd:PRK07768  228 PMDFLRDPLLWaeliSKYRGTmtaaPNFAYALLARRLRRQAKPGAfdlsslRFALNGAEPIDPADVEDLLDagarfgLRP 307

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1824 DAeVINHYGPTETVI---------GRVVHPVR----EAGELAVDSPLP-------LGRPLGETRLQVLDAWLEAVPVGAV 1883
Cdd:PRK07768  308 EA-ILPAYGMAEATLavsfspcgaGLVVDEVDadllAALRRAVPATKGntrrlatLGPPLPGLEVRVVDEDGQVLPPRGV 386

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1884 GELFIGGDGIARGYlgrpaLTAEKFLP--DPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARM 1961
Cdd:PRK07768  387 GVIELRGESVTPGY-----LTMDGFIPaqDADG-----WLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAA 456

                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1962 AEHPGVEQ----AVVLVRDQQLVGWFIPAE---DHPPVTVSALRR-FCAEQLPEFMV-PNQWVALDA--FPLTPHGKVNH 2030
Cdd:PRK07768  457 ARVEGVRPgnavAVRLDAGHSREGFAVAVEsnaFEDPAEVRRIRHqVAHEVVAEVGVrPRNVVVLGPgsIPKTPSGKLRR 536


                  ..
gi 653678460 2031 RA 2032
Cdd:PRK07768  537 AN 538
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
 1879-1998 7.45e-07

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 54.75  E-value: 7.45e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1879 PVGAVGELFIGGDGIARGYLGRPALTAEKF-----LPDPAG------EPGARMYRTGDLVRWRgDGLLDFVGRVDDQVKL 1947
Cdd:PRK12476  425 PDGEVGEIWLHGDNIGRGYWGRPEETERTFgaklqSRLAEGshadgaADDGTWLRTGDLGVYL-DGELYITGRIADLIVI 503

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1948 RGYRIELGEIEARMAEhpgveqAVVLVRDQQLVGWFIPAEDHPPVTVSALR 1998
Cdd:PRK12476  504 DGRNHYPQDIEATVAE------ASPMVRRGYVTAFTVPAEDNERLVIVAER 548
PksD COG3321
Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites ...
 1070-1607 1.07e-06

Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442550 [Multi-domain]  Cd Length: 1386  Bit Score: 54.49  E-value: 1.07e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1070 DAVHLAPVPRDEPLPLSPMQ-ESIWLADQVSAGDSVYNVPLALRLIGPLDRGALRRTLNRVVDRHEMLRTRIHPGPDGMP 1148
Cdd:COG3321   850 SALYPGRGRRRVPLPTYPFQrEDAAAALLAAALAAALAAAAALGALLLAALAAALAAALLALAAAAAAALALAAAALAAL 929

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1149 VQVADPAGGGAALTERDAAPGTDLAQLLLAEAALGFTLATEHPLR--AVLWRLDEREHVLLLTAHHVAVDAWSMDLIQRD 1226
Cdd:COG3321   930 LALVALAAAAAALLALAAAAAAAAAALAAAEAGALLLLAAAAAAAaaAAAAAAAAAAAAAAAAAAALAAAAALALLAAAA 1009

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1227 LTELYAADTGHREPELDEPALAQADYAVWQRQSAQRGRYAAELDFWRTELTGAVATEVAGDLPRPATPGGGGGAVEFALA 1306
Cdd:COG3321  1010 LLLAAAAAAAALLALAALLAAAAAALAAAAAAAAAAAALAALAAAAAAAAALALALAALLLLAALAELALAAAALALAAA 1089

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1307 PRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDnLVGCLVNTVVLRGDLSGAPTFHELL 1386
Cdd:COG3321  1090 LAAAALALALAALAAALLLLALLAALALAAAAAALLALAALLAAAAAAAALAAA-AAAAAALALAAAAAALAAALAAALL 1168

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1387 RRTHARTADALAHQELPFEHLVADRGAGNGPLFRimysfsSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTLE 1466
Cdd:COG3321  1169 AAAALLLALALALAAALAAALAGLAALLLAALLA------ALLAALLALALAALAAAAAALLAAAAAAAALALLALAAAA 1242

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1467 GVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRTSRWGRSEQHVGDDRTVHQLVEAQADRTPG 1546
Cdd:COG3321  1243 AAVAALAAAAAALLAALAALALLAAAAGLAALAAAAAAAAAALALAAAAAAAAAALAALLAAAAAAAAAAAAAAAAAALA 1322

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1547 WTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVGPETPVLLLLDRSPELVVAMLAVL 1607
Cdd:COG3321  1323 AALLAAALAALAAAVAAALALAAAAAAAAAAAAAAAAAAALAAAAGAAAAAAALALAALAA 1383
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
 516-921 1.84e-06

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 53.20  E-value: 1.84e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  516 TLSYRELDERANRLARLLMERGAGAETFVAVLLPRSADLLVTLLAVIKAGAAYLPIDPDFPVERIAYLL--SDARpVLVV 593
Cdd:cd17641    11 EFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLnyTGAR-VVIA 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  594 TDAATADRLGP--DDITGL---VVLEETDTGGY------------------PATEP----PAVPAGH---SAYVIYTSGS 643
Cdd:cd17641    90 EDEEQVDKLLEiaDRIPSVryvIYCDPRGMRKYddprlisfedvvalgralDRRDPglyeREVAAGKgedVAVLCTTSGT 169

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  644 TGRPKGVVITRAALDNFLAAMAERFPLTAEDRVLAT--------------------TTVSF------------DIAGLEL 691
Cdd:cd17641   170 TGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVlplpwigeqmysvgqalvcgFIVNFpeepetmmedlrEIGPTFV 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  692 YLPLRSGAGVvLAD-----ADTARNPAALIDLA-GRHRVTLAQ----ATPTLWQALVPELSGPALAGI-----------R 750
Cdd:cd17641   250 LLPPRVWEGI-AADvrarmMDATPFKRFMFELGmKLGLRALDRgkrgRPVSLWLRLASWLADALLFRPlrdrlgfsrlrS 328

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  751 VLVGGEALPAELARRLGDAGAEVTNMYGPTETTIWSTSGPTseDSIRRGSIGVPIDNTQVYVLDAnlhpapigvlGELHI 830
Cdd:cd17641   329 AATGGAALGPDTFRFFHAIGVPLKQLYGQTELAGAYTVHRD--GDVDPDTVGVPFPGTEVRIDEV----------GEILV 396

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  831 AGEGLARGYWNRPGLTAEKFLPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKL-RGFRIELGEIETTLiNAGPV 909
Cdd:cd17641   397 RSPGVFVGYYKNPEATAEDFDEDGW-------LHTGDAGYFKENGHLVVIDRAKDVGTTsDGTRFSPQFIENKL-KFSPY 468

                         490
                  ....*....|..
gi 653678460  910 RRAAAVVREDRP 921
Cdd:cd17641   469 IAEAVVLGAGRP 480
PaaK cd05913
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ...
 605-903 2.34e-06

Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.


Pssm-ID: 341239 [Multi-domain]  Cd Length: 425  Bit Score: 52.63  E-value: 2.34e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  605 DDITGLVVLEETDTGGYPATEPPAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMA---ERFPLTAEDRVLATTT 681
Cdd:cd05913    51 DDLRKLPFTTKEDLRDNYPFGLFAVPREKVVRIHASSGTTGKPTVVGYTKNDLDVWAELVArclDAAGVTPGDRVQNAYG 130

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  682 VSFDIAGLELYLPL-RSGAGVVLADA-DTARNPAALIDLagrhRVTLAQATPTLWQALVPELS----GPALAGIRV-LVG 754
Cdd:cd05913   131 YGLFTGGLGFHYGAeRLGALVIPAGGgNTERQLQLIKDF----GPTVLCCTPSYALYLAEEAEeegiDPRELSLKVgIFG 206

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  755 GEALPAELARRLGDA-GAEVTNMYGPTETTiwstsGP-TSEDSIRRGSIGVPIDNTQVYVLD-ANLHPAPIGVLGELHIA 831
Cdd:cd05913   207 AEPWTEEMRKRIERRlGIKAYDIYGLTEII-----GPgVAFECEEKDGLHIWEDHFIPEIIDpETGEPVPPGEVGELVFT 281

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  832 geglargywnrpGLTAEkflpdpfGPPGTRmYRTGDLVR--WSAAG-------DLEYLGRTDHQVKLRGFRIELGEIETT 902
Cdd:cd05913   282 ------------TLTKE-------AMPLIR-YRTRDITRllPGPCPcgrthrrIDRITGRSDDMLIIRGVNVFPSQIEDV 341


                  .
gi 653678460  903 L 903
Cdd:cd05913   342 L 342
PaaK COG1541
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ...
 1691-1974 2.71e-06

Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];


Pssm-ID: 441150 [Multi-domain]  Cd Length: 423  Bit Score: 52.46  E-value: 2.71e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1691 TSGSTGQPKGVAVEHIGIVRylsWA---AASYPTAG-RRGTLAHSSVGFDLTMSALFepLVSG---RGVTLMPA-----D 1758
Cdd:COG1541    91 SSGTTGKPTVVGYTRKDLDR---WAelfARSLRAAGvRPGDRVQNAFGYGLFTGGLG--LHYGaerLGATVIPAgggntE 165

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1759 ATLaDLAEELsGPlayDYIRLTPSHLRHLVGHWTGQELPPAA---RGWVVGGETLDPALVKQLLElRPDAEVINHYGPTE 1835
Cdd:COG1541   166 RQL-RLMQDF-GP---TVLVGTPSYLLYLAEVAEEEGIDPRDlslKKGIFGGEPWSEEMRKEIEE-RWGIKAYDIYGLTE 239

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1836 TVIGrvvhpvreageLAVDSPLPLGRPLGETRL--QVLD-AWLEAVPVGAVGELFIggdgiargylgrPALTAEKFlpdp 1912
Cdd:COG1541   240 VGPG-----------VAYECEAQDGLHIWEDHFlvEIIDpETGEPVPEGEEGELVV------------TTLTKEAM---- 292

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1913 agePGARmYRTGDLVRWRGDGL--------LDFV-GRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLV 1974
Cdd:COG1541   293 ---PLIR-YRTGDLTRLLPEPCpcgrthprIGRIlGRADDMLIIRGVNVFPSQIEEVLLRIPEVGPEYQIV 359
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
 1557-1972 4.09e-06

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 52.09  E-value: 4.09e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1557 LTFAGLDAQANRLAHALHtgALGAPPvgpETPVLLLLDRSPELVVAMLAVLKAGGYFIPVdpgYPmarlTRIADTVtpAL 1636
Cdd:cd05932     7 FTWGEVADKARRLAAALR--ALGLEP---GSKIALISKNCAEWFITDLAIWMAGHISVPL---YP----TLNPDTI--RY 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1637 VLTRTGQGGCLPG---------AEVFGDVPVVAL--------DRVADRLTAM-PDQRPEVTVDPRGLAYSIFTSGSTGQP 1698
Cdd:cd05932    73 VLEHSESKALFVGklddwkamaPGVPEGLISISLpppsaancQYQWDDLIAQhPPLEERPTRFPEQLATLIYTSGTTGQP 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1699 KGV-------------AVEHIGIV---RYLSWAAasyptagrrgtLAHSSVGFDLTMSALFEplvsgrGVTLMpadatla 1762
Cdd:cd05932   153 KGVmltfgsfawaaqaGIEHIGTEendRMLSYLP-----------LAHVTERVFVEGGSLYG------GVLVA------- 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1763 dLAEELSGPLAyDYIRLTPSHLRHLvghwtgqelppaARGWVVGGETLDPALVKQLLELRPDAEVINhygpteTVIGRVV 1842
Cdd:cd05932   209 -FAESLDTFVE-DVQRARPTLFFSV------------PRLWTKFQQGVQDKIPQQKLNLLLKIPVVN------SLVKRKV 268

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1843 HP------VREAGELAvdSPLPLG-----RPLGetrLQVLDAWL----------------------EAVP-----VGAVG 1884
Cdd:cd05932   269 LKglgldqCRLAGCGS--APVPPAllewyRSLG---LNILEAYGmtenfayshlnypgrdkigtvgNAGPgvevrISEDG 343

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1885 ELFIGGDGIARGYLGRPALTAEKFLPDPagepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKL-RGYRIELGEIEARMAE 1963
Cdd:cd05932   344 EILVRSPALMMGYYKDPEATAEAFTADG-------FLRTGDKGELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLAE 416


                  ....*....
gi 653678460 1964 HPGVEQAVV 1972
Cdd:cd05932   417 HDRVEMVCV 425
PldB COG2267
Lysophospholipase, alpha-beta hydrolase superfamily [Lipid transport and metabolism];
2139-2288 9.17e-06

Lysophospholipase, alpha-beta hydrolase superfamily [Lipid transport and metabolism];


Pssm-ID: 441868 [Multi-domain]  Cd Length: 221  Bit Score: 49.23  E-value: 9.17e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2139 GHTGTTRTVVTLRGEGDGRP-VFCVHPSGGGVAWYLPLARAL-PAGHPVHAFQPLGMDGREAPAETIEDMAAgYLADLRL 2216
Cdd:COG2267    10 TRDGLRLRGRRWRPAGSPRGtVVLVHGLGEHSGRYAELAEALaAAGYAVLAFDLRGHGRSDGPRGHVDSFDD-YVDDLRA 88

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 653678460 2217 V------QPEGPYVVVGWSLGGTIAfemARQLDRLGEPVQ-LILLEPTLPEvartiDLHRPARDSYLRGADLAERILRL 2288
Cdd:COG2267    89 AldalraRPGLPVVLLGHSMGGLIA---LLYAARYPDRVAgLVLLAPAYRA-----DPLLGPSARWLRALRLAEALARI 159
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
600-976 4.58e-05

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 48.61  E-value: 4.58e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  600 DRLGPDDiTGLVVLEETDTGGYPATEP-PAVPAGHSAYVIYTSGSTGRPKGVVITRAALDNFLAAMAERFPLT-AEDRVL 677
Cdd:PRK05851  120 ERLRAVD-SSVTVHDLATAAHTNRSASlTPPDSGGPAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVGLDaATDVGC 198

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  678 A----------TTTVSFDIAGLELYL---------PLR-------SGAGVVLAdadtarnPAALIDLAGRHrvtlaqatp 731
Cdd:PRK05851  199 SwlplyhdmglAFLLTAALAGAPLWLapttafsasPFRwlswlsdSRATLTAA-------PNFAYNLIGKY--------- 262

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  732 tlwqalVPELSGPALAGIRV-LVGGEALPAELARRLG--------DAGAeVTNMYGPTETTIWST-SGP---------TS 792
Cdd:PRK05851  263 ------ARRVSDVDLGALRVaLNGGEPVDCDGFERFAtamapfgfDAGA-AAPSYGLAESTCAVTvPVPgiglrvdevTT 335

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  793 ED--SIRRGSI-GVPIDNTQVYVlDANLHPAPIGV--LGELHIAGEGLARGYWNRPGLTAEKFLPdpfgppgtrmyrTGD 867
Cdd:PRK05851  336 DDgsGARRHAVlGNPIPGMEVRI-SPGDGAAGVAGreIGEIEIRGASMMSGYLGQAPIDPDDWFP------------TGD 402

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  868 LVRWSAAGdLEYLGRTDHQVKLRGFRIELGEIETTlinAGPVR--RAAAVV------REDRPGDLRLVAYVIPDGPVApd 939
Cdd:PRK05851  403 LGYLVDGG-LVVCGRAKELITVAGRNIFPTEIERV---AAQVRgvREGAVVavgtgeGSARPGLVIAAEFRGPDEAGA-- 476

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|.
gi 653678460  940 alRTELSRTLPDY--MIPAVIVPVP--DFPTTPNGKLDRAA 976
Cdd:PRK05851  477 --RSEVVQRVASEcgVVPSDVVFVApgSLPRTSSGKLRRLA 515
PRK05850 PRK05850
acyl-CoA synthetase; Validated
 1598-1949 5.52e-05

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 48.40  E-value: 5.52e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1598 ELVVAMLAVLKAGgyFIPVDPGYPMARLT--RI----ADTvTPALVLT------RTGQGGCLPGAEVfgDVPVVALDRVA 1665
Cdd:PRK05850   71 EYIVAFLGALQAG--LIAVPLSVPQGGAHdeRVsavlRDT-SPSVVLTtsavvdDVTEYVAPQPGQS--APPVIEVDLLD 145

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1666 drltamPDQRPEVTVDPRGL---AYSIFTSGSTGQPKGVAVEHIG-IVRYLSWAAASYPTAGRRGTLAHSSVGF-----D 1736
Cdd:PRK05850  146 ------LDSPRGSDARPRDLpstAYLQYTSGSTRTPAGVMVSHRNvIANFEQLMSDYFGDTGGVPPPDTTVVSWlpfyhD 219

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1737 L-TMSALFEPLVSGRGVTLMPADATLADLAEelsgplaydYIRLTPSHLRHlvghWT-----GQELppAAR--------- 1801
Cdd:PRK05850  220 MgLVLGVCAPILGGCPAVLTSPVAFLQRPAR---------WMQLLASNPHA----FSaapnfAFEL--AVRktsdddmag 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1802 -------GWVVGGETLDPALVKQLLE------LRPDAeVINHYGPTE-TV---IGRVVHP---VR-EAGEL----AVDSP 1856
Cdd:PRK05850  285 ldlggvlGIISGSERVHPATLKRFADrfapfnLRETA-IRPSYGLAEaTVyvaTREPGQPpesVRfDYEKLsaghAKRCE 363

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1857 LPLGRPL---GETRLQVL-----DAWLEAvPVGAVGELFIGGDGIARGYLGRPALTAEKF---LPDP-AGEPGARMYRTG 1924
Cdd:PRK05850  364 TGGGTPLvsyGSPRSPTVrivdpDTCIEC-PAGTVGEIWVHGDNVAAGYWQKPEETERTFgatLVDPsPGTPEGPWLRTG 442

                         410       420
                  ....*....|....*....|....*.
gi 653678460 1925 DL-VRWrgDGLLDFVGRVDDQVKLRG 1949
Cdd:PRK05850  443 DLgFIS--EGELFIVGRIKDLLIVDG 466
Abhydrolase_6 pfam12697
Alpha/beta hydrolase family; This family contains alpha/beta hydrolase enzymes of diverse ...
 2159-2306 6.32e-05

Alpha/beta hydrolase family; This family contains alpha/beta hydrolase enzymes of diverse specificity.


Pssm-ID: 463673 [Multi-domain]  Cd Length: 211  Bit Score: 46.70  E-value: 6.32e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2159 VFCVHpsgGGVAWYLPLARALPAGHPVHAFQPLGMDGREAPAETIEDMAAgYLADLRLVQPEGPYVVVGWSLGGTIAFEM 2238
Cdd:pfam12697    1 VVLVH---GAGLSAAPLAALLAAGVAVLAPDLPGHGSSSPPPLDLADLAD-LAALLDELGAARPVVLVGHSLGGAVALAA 76

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  2239 ARQLDRLGepvqlILLEPT---LPEVARTIDLHRP-----ARDSYLRGADLAERILRLPAGDPEGDRLRAELTRLL 2306
Cdd:pfam12697   77 AAAALVVG-----VLVAPLaapPGLLAALLALLARlgaalAAPAWLAAESLARGFLDDLPADAEWAAALARLAALL 147
PksD COG3321
Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites ...
 1226-1773 1.09e-04

Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 442550 [Multi-domain]  Cd Length: 1386  Bit Score: 47.94  E-value: 1.09e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1226 DLTELYAADTGHRepeLDEPAlaqadYAvWQRQSAQRGRYAAELdfwRTELTGAVATEVAGDLPRPATPGGGGGAVEFAL 1305
Cdd:COG3321   848 DWSALYPGRGRRR---VPLPT-----YP-FQREDAAAALLAAAL---AAALAAAAALGALLLAALAAALAAALLALAAAA 915

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1306 APRQIQGIRELARRCGTTVSTVVLSALQTLLYRITGGADVVIGSTVSGRGNPRLDNLVGCLVNTVVLRGDLSGAPTFHEL 1385
Cdd:COG3321   916 AAALALAAAALAALLALVALAAAAAALLALAAAAAAAAAALAAAEAGALLLLAAAAAAAAAAAAAAAAAAAAAAAAAAAA 995

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1386 LRRTHARTADALAHQELPFEHLVADRGAGNGPLFRimysfsSQEPAGRSAGDVDLEPVPVPVTTCKFDLVLTVVDGGSTL 1465
Cdd:COG3321   996 LAAAAALALLAAAALLLAAAAAAAALLALAALLAA------AAAALAAAAAAAAAAAALAALAAAAAAAAALALALAALL 1069

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1466 EGVLEYADDLYLPGTAERLVTSLTNLLAAAVRTPELAVTRLAITSESDTVRTSRWGRSEQHVGDDRTVHQLVEAQADRTP 1545
Cdd:COG3321  1070 LLAALAELALAAAALALAAALAAAALALALAALAAALLLLALLAALALAAAAAALLALAALLAAAAAAAALAAAAAAAAA 1149

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1546 GWTALRTGDRSLTFAGLDAQANRLAHALHTGALGAPPVGPETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDPGYPMARL 1625
Cdd:COG3321  1150 LALAAAAAALAAALAAALLAAAALLLALALALAAALAAALAGLAALLLAALLAALLAALLALALAALAAAAAALLAAAAA 1229

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1626 TRIADTVTPALVLTRTGQGGCLPGAEVFGDVPVVALDRVADRLTAMPDQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEH 1705
Cdd:COG3321  1230 AAALALLALAAAAAAVAALAAAAAALLAALAALALLAAAAGLAALAAAAAAAAAALALAAAAAAAAAALAALLAAAAAAA 1309

                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1706 IGIVRYLSWAAASYPTAGRRGTLAHSSVGFDLTMSALFEPLVSGRGVTLMPADATLADLAEELSGPLA 1773
Cdd:COG3321  1310 AAAAAAAAAAALAAALLAAALAALAAAVAAALALAAAAAAAAAAAAAAAAAAALAAAAGAAAAAAALA 1377
Abhydrolase_1 pfam00561
alpha/beta hydrolase fold; This catalytic domain is found in a very wide range of enzymes.
 2158-2305 1.23e-04

alpha/beta hydrolase fold; This catalytic domain is found in a very wide range of enzymes.


Pssm-ID: 395444 [Multi-domain]  Cd Length: 245  Bit Score: 45.96  E-value: 1.23e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2158 PVFCVHPSGGGVA--WYlPLARAL-PAGHPVHAFQPLGMdGREAPAETIEDMAAGYLAD--LRLVQPEG--PYVVVGWSL 2230
Cdd:pfam00561    1 PPVLLLHGLPGSSdlWR-KLAPALaRDGFRVIALDLRGF-GKSSRPKAQDDYRTDDLAEdlEYILEALGleKVNLVGHSM 78

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 653678460  2231 GGTIAFEMARQLDrlgEPVQ-LILLEPTLPEVArTIDLHRPARDSYLRGAD-LAERILRLPAGDPEGDRLRAELTRL 2305
Cdd:pfam00561   79 GGLIALAYAAKYP---DRVKaLVLLGALDPPHE-LDEADRFILALFPGFFDgFVADFAPNPLGRLVAKLLALLLLRL 151
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
 1542-1701 1.37e-04

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 47.04  E-value: 1.37e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1542 DRTpgWTALRTGD---RSLTFAGLDAQANRLAHALHTGALGAppvgpETPVLLLLDRSPELVVAMLAVLKAGGYFIPVDP 1618
Cdd:cd05921    10 DRT--WLAEREGNggwRRVTYAEALRQVRAIAQGLLDLGLSA-----ERPLLILSGNSIEHALMALAAMYAGVPAAPVSP 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1619 GYPM-----ARLTRIADTVTPALVLTRTGQ--GGCLpGAEVFGDVPVVALDR-VADR-------------LTAMPDQRPE 1677
Cdd:cd05921    83 AYSLmsqdlAKLKHLFELLKPGLVFAQDAApfARAL-AAIFPLGTPLVVSRNaVAGRgaisfaelaatppTAAVDAAFAA 161

                         170       180
                  ....*....|....*....|....
gi 653678460 1678 VTvdPRGLAYSIFTSGSTGQPKGV 1701
Cdd:cd05921   162 VG--PDTVAKFLFTSGSTGLPKAV 183
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
 1831-1984 1.70e-04

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 47.03  E-value: 1.70e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1831 YGPTETVIGRVVHPvreAGELAVDSplpLGRPLGETrlqvldawleAVPVGAVGELFIGGDGIARGYLGRPALTAEKFLP 1910
Cdd:cd17641   355 YGQTELAGAYTVHR---DGDVDPDT---VGVPFPGT----------EVRIDEVGEILVRSPGVFVGYYKNPEATAEDFDE 418

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460 1911 DpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKL-RGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGWFI 1984
Cdd:cd17641   419 D-------GWLHTGDAGYFKENGHLVVIDRAKDVGTTsDGTRFSPQFIENKLKFSPYIAEAVVLGAGRPYLTAFI 486
PaaK cd05913
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ...
 1691-1968 2.19e-04

Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.


Pssm-ID: 341239 [Multi-domain]  Cd Length: 425  Bit Score: 46.08  E-value: 2.19e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1691 TSGSTGQPKGVAVEHIGIvRYLSWAAASYPTA--GRRGTLAHSSVGFDLTMSALfePLVSG---RGVTLMPADATLADLA 1765
Cdd:cd05913    86 SSGTTGKPTVVGYTKNDL-DVWAELVARCLDAagVTPGDRVQNAYGYGLFTGGL--GFHYGaerLGALVIPAGGGNTERQ 162

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1766 EELSGPLAYDYIRLTPSHLRHLV-----GHWTGQELPPaaRGWVVGGETLDPALvKQLLELRPDAEVINHYGPTETVIGR 1840
Cdd:cd05913   163 LQLIKDFGPTVLCCTPSYALYLAeeaeeEGIDPRELSL--KVGIFGAEPWTEEM-RKRIERRLGIKAYDIYGLTEIIGPG 239

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1841 VVHPVREAGELAVDSPLPLgrplgetrLQVLD-AWLEAVPVGAVGELFIggdgiargylgrPALTAEkflpdpaGEPGAR 1919
Cdd:cd05913   240 VAFECEEKDGLHIWEDHFI--------PEIIDpETGEPVPPGEVGELVF------------TTLTKE-------AMPLIR 292

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 653678460 1920 mYRTGDLVRWRGDG---------LLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVE 1968
Cdd:cd05913   293 -YRTRDITRLLPGPcpcgrthrrIDRITGRSDDMLIIRGVNVFPSQIEDVLLKIPGLG 349
PRK09294 PRK09294
phthiocerol/phthiodiolone dimycocerosyl transferase;
73-207 2.58e-04

phthiocerol/phthiodiolone dimycocerosyl transferase;


Pssm-ID: 181765 [Multi-domain]  Cd Length: 416  Bit Score: 45.86  E-value: 2.58e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460   73 LAVRLTGPLDVDALRAALDAVTARHEALRTVFRLGPDGEPVQQTAPAAPVALPVIDvTEADLPQAlrtaaeqPFDLERGP 152
Cdd:PRK09294   26 YTAHLRGVLDIDALSDAFDALLRAHPVLAAHLEQDSDGGWELVADDLLHPGIVVVD-GDAARPLP-------ELQLDQGV 97

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 653678460  153 LLRACLHRIGAKQHVLLVTVHHIVLDGWSCGLLLADLARAYAGELPAGVAAPAFR 207
Cdd:PRK09294   98 SLLALDVVPDDGGARVTLYIHHSIADAHHSASLLDELWSRYTDVVTTGDPGPIRP 152
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
 777-905 2.59e-04

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 46.25  E-value: 2.59e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  777 YGPTETTiwstsGP---TSEDSIRRGSIGVPID-NTQVYVLDANLHPAPiGVL--GELHIAGEGLARGYWNRPGLTAEKF 850
Cdd:PTZ00342  493 YGLTETT-----GPifvQHADDNNTESIGGPISpNTKYKVRTWETYKAT-DTLpkGELLIKSDSIFSGYFLEKEQTKNAF 566

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460  851 LPDPFgppgtrmYRTGDLVRWSAAGDLEYLGRTDHQVKLrgfriELGE-IETTLIN 905
Cdd:PTZ00342  567 TEDGY-------FKTGDIVQINKNGSLTFLDRSKGLVKL-----SQGEyIETDMLN 610
PRK14875 PRK14875
acetoin dehydrogenase E2 subunit dihydrolipoyllysine-residue acetyltransferase; Provisional
 2132-2240 2.60e-04

acetoin dehydrogenase E2 subunit dihydrolipoyllysine-residue acetyltransferase; Provisional


Pssm-ID: 184875 [Multi-domain]  Cd Length: 371  Bit Score: 45.71  E-value: 2.60e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2132 LDTVFGAGHTGTT--RTVVTLR-GEGDGRPVFCVHPSGGGVAWYLPLARALPAGHPVHAFQpL---GMDGREAPAETIED 2205
Cdd:PRK14875  104 EEDAGPAPRKARIggRTVRYLRlGEGDGTPVVLIHGFGGDLNNWLFNHAALAAGRPVIALD-LpghGASSKAVGAGSLDE 182

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 653678460 2206 MAAGYLADLRLVQPEgPYVVVGWSLGGTIAFEMAR 2240
Cdd:PRK14875  183 LAAAVLAFLDALGIE-RAHLVGHSMGGAVALRLAA 216
PLN02654 PLN02654
acetate-CoA ligase
 1915-2033 6.52e-04

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 44.89  E-value: 6.52e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1915 EPGARMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVLVRDQQLVGW----FIPAEDHP 1990
Cdd:PLN02654  509 KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQgiyaFVTLVEGV 588

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 653678460 1991 PVTV---SALRRFCAEQLPEFMVPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PLN02654  589 PYSEelrKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 634
hsFATP2a_ACSVL_like cd05938
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ...
 1553-1710 6.86e-04

Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341261 [Multi-domain]  Cd Length: 537  Bit Score: 44.97  E-value: 6.86e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1553 GDRSLTFAGLDAQANRLAHALHTgalgAPPVGPETPVLLLLDRSPELVVAMLAVLKAggyfipvdpGYPMARL------- 1625
Cdd:cd05938     2 EGETYTYRDVDRRSNQAARALLA----HAGLRPGDTVALLLGNEPAFLWIWLGLAKL---------GCPVAFLntnirsk 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1626 -----------------TRIADTVTPALVLTRTGqggclpGAEVF---GDVPVVALDRVADRLTAMPDQRP----EVTVD 1681
Cdd:cd05938    69 sllhcfrccgakvlvvaPELQEAVEEVLPALRAD------GVSVWylsHTSNTEGVISLLDKVDAASDEPVpaslRAHVT 142

                         170       180
                  ....*....|....*....|....*....
gi 653678460 1682 PRGLAYSIFTSGSTGQPKGVAVEHIGIVR 1710
Cdd:cd05938   143 IKSPALYIYTSGTTGLPKAARISHLRVLQ 171
PRK08162 PRK08162
acyl-CoA synthetase; Validated
 1870-2028 8.70e-04

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 44.55  E-value: 8.70e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1870 VLD-AWLEAVPVGA--VGELFIGGDGIARGYLGRPALTAEKFlpdpAGEpgarMYRTGDLVRWRGDGLLDFVGRVDDQVK 1946
Cdd:PRK08162  372 VLDpDTMQPVPADGetIGEIMFRGNIVMKGYLKNPKATEEAF----AGG----WFHTGDLAVLHPDGYIKIKDRSKDIII 443

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1947 LRGYRIELGEIEARMAEHPGVEQAVVLVR-DQQlvgW------FIPAEDHPPVTVSALRRFCAEQLPEFMVPNQwVALDA 2019
Cdd:PRK08162  444 SGGENISSIEVEDVLYRHPAVLVAAVVAKpDPK---WgevpcaFVELKDGASATEEEIIAHCREHLAGFKVPKA-VVFGE 519


                  ....*....
gi 653678460 2020 FPLTPHGKV 2028
Cdd:PRK08162  520 LPKTSTGKI 528
bioH TIGR01738
pimelyl-[acyl-carrier protein] methyl ester esterase; This CoA-binding enzyme is required for ...
 2154-2239 9.88e-04

pimelyl-[acyl-carrier protein] methyl ester esterase; This CoA-binding enzyme is required for the production of pimeloyl-coenzyme A, the substrate of the BioF protein early in the biosynthesis of biotin. Its exact function is unknown, but is proposed in ref 2. This enzyme belongs to the alpha/beta hydrolase fold family (pfam00561). Members of this family are restricted to the Proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin]


Pssm-ID: 273783 [Multi-domain]  Cd Length: 245  Bit Score: 43.27  E-value: 9.88e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460  2154 GDGRP-VFCVHPSGGGVAWYLPLARALPAGHPVHAFQPLGM-DGREAPAETIEDMAAGYLAdlrlvQPEGPYVVVGWSLG 2231
Cdd:TIGR01738    1 GQGNVhLVLIHGWGMNAEVFRCLDEELSAHFTLHLVDLPGHgRSRGFGPLSLADMAEAIAA-----QAPDPAIWLGWSLG 75


                   ....*...
gi 653678460  2232 GTIAFEMA 2239
Cdd:TIGR01738   76 GLVALHIA 83
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
 1679-2033 1.08e-03

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 44.36  E-value: 1.08e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1679 TVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVryLSWAAASYPTAgrRGTLAHSSV----------GFDLTMSAlfePLVS 1748
Cdd:PRK06018  173 TFDENTAAGMCYTSGTTGDPKGVLYSHRSNV--LHALMANNGDA--LGTSAADTMlpvvplfhanSWGIAFSA---PSMG 245

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1749 GRGVtlMP-ADATLADLAEELSGPlAYDYIRLTPSHLRHLVGHWTGQELP-PAARGWVVGGETLDPALVKQLLELrpDAE 1826
Cdd:PRK06018  246 TKLV--MPgAKLDGASVYELLDTE-KVTFTAGVPTVWLMLLQYMEKEGLKlPHLKMVVCGGSAMPRSMIKAFEDM--GVE 320

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1827 VINHYGPTET----VIGRVVHPVREA-GELAVDSPLPLGRPLGETRLQVLDAWLEAVPVG--AVGELFIGGDGIARGYLG 1899
Cdd:PRK06018  321 VRHAWGMTEMsplgTLAALKPPFSKLpGDARLDVLQKQGYPPFGVEMKITDDAGKELPWDgkTFGRLKVRGPAVAAAYYR 400

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1900 rpaltAEKFLPDPAGepgarMYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGVEQAVVlvrdqql 1979
Cdd:PRK06018  401 -----VDGEILDDDG-----FFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAV------- 463

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 653678460 1980 VGWFIPAEDHPPVTVSALRR---FCAEQLPEFM--------VPNQWVALDAFPLTPHGKVNHRAL 2033
Cdd:PRK06018  464 IGVYHPKWDERPLLIVQLKPgetATREEILKYMdgkiakwwMPDDVAFVDAIPHTATGKILKTAL 528
PKS_PP smart00823
Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the ...
 2055-2116 1.19e-03

Phosphopantetheine attachment site; Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups.


Pssm-ID: 214834 [Multi-domain]  Cd Length: 86  Bit Score: 39.93  E-value: 1.19e-03
                            10        20        30        40        50        60
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653678460   2055 EQLLAELWSTVLG---TDRIGIEDNFFDLGGTSISVIQLSGACRRA-GVEISPKDVFEQPTVRGQA 2116
Cdd:smart00823   14 LDLVREQVAAVLGhaaAEAIDPDRPFRDLGLDSLMAVELRNRLEAAtGLRLPATLVFDHPTPAALA 79
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
 1920-1972 2.18e-03

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 43.21  E-value: 2.18e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 653678460 1920 MYRTGDLVRWRGDGLLDFVGRVDDQVKLRGYRIELGEIEARMAEHPGV-EQAVV 1972
Cdd:PRK00174  484 MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVaEAAVV 537
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
 1673-1972 2.82e-03

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 42.97  E-value: 2.82e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1673 DQRPEVTVDPRGLAYSIFTSGSTGQPKGVAVEHIGIVrylSWAAASYPTAGRRGTLAHSSVGFD-LTMSALFEPLV---- 1747
Cdd:cd05927   104 NKVPPPPPKPEDLATICYTSGTTGNPKGVMLTHGNIV---SNVAGVFKILEILNKINPTDVYISyLPLAHIFERVVealf 180

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1748 --SGRGV-------------------TLMPA---------DATLADLAEelSGPL-------AYDY--IRLTPSHLRH-- 1786
Cdd:cd05927   181 lyHGAKIgfysgdirlllddikalkpTVFPGvprvlnriyDKIFNKVQA--KGPLkrklfnfALNYklAELRSGVVRAsp 258

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1787 ----LVGHWTGQELPPAARGWVVGGETLDPAlVKQLLELRPDAEVINHYGPTETV-IGRVVHP-VREAGElaVDSPLPlg 1860
Cdd:cd05927   259 fwdkLVFNKIKQALGGNVRLMLTGSAPLSPE-VLEFLRVALGCPVLEGYGQTECTaGATLTLPgDTSVGH--VGGPLP-- 333

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 1861 rplgetrlqVLDAWLEAVP---------VGAvGELFIGGDGIARGYLGRPALTAEKFlpDPAGepgarMYRTGDLVRWRG 1931
Cdd:cd05927   334 ---------CAEVKLVDVPemnydakdpNPR-GEVCIRGPNVFSGYYKDPEKTAEAL--DEDG-----WLHTGDIGEWLP 396

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 653678460 1932 DGLLDFVGRVDDQVKL-RGYRIELGEIEARMAEHPGVEQAVV 1972
Cdd:cd05927   397 NGTLKIIDRKKNIFKLsQGEYVAPEKIENIYARSPFVAQIFV 438
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
 1884-1953 5.47e-03

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 42.01  E-value: 5.47e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653678460 1884 GELFIGGDGIARGYLGRPALTAEKFLPDpagepgaRMYRTGDLVRWRGDGLLDFVGRVDDQVKL-RGYRIE 1953
Cdd:PTZ00342  542 GELLIKSDSIFSGYFLEKEQTKNAFTED-------GYFKTGDIVQINKNGSLTFLDRSKGLVKLsQGEYIE 605
EstA COG1075
Triacylglycerol esterase/lipase EstA, alpha/beta hydrolase fold [Lipid transport and ...
 2155-2244 8.32e-03

Triacylglycerol esterase/lipase EstA, alpha/beta hydrolase fold [Lipid transport and metabolism];


Pssm-ID: 440693 [Multi-domain]  Cd Length: 106  Bit Score: 38.27  E-value: 8.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653678460 2155 DGRPVFCVHPSGGGVAWYLPLARALPA-GHPVHAFQplgMDGREAPAETIEDMAAGYLADLRLVQPEGPYVVVGWSLGGT 2233
Cdd:COG1075     4 TRYPVVLVHGLGGSAASWAPLAPRLRAaGYPVYALN---YPSTNGSIEDSAEQLAAFVDAVLAATGAEKVDLVGHSMGGL 80

                          90
                  ....*....|.
gi 653678460 2234 IAFEMARQLDR 2244
Cdd:COG1075    81 VARYYLKRLGG 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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