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Conserved domains on  [gi|544848476|ref|WP_021263644|]
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MULTISPECIES: type II secretion system protein [Pseudomonas]

Protein Classification

type II secretion system protein( domain architecture ID 11450365)

type II secretion system protein similar to Pseudomonas aeruginosa type II secretion system protein H, which is involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins; required for the translocation of a variety of enzymes across the outer membrane; pilin similar to fimbrial protein PilE, which is required for pilus biogenesis, as well as fimbrial protein PilA, which is responsible for the adhesive property of the pilus

Gene Ontology:  GO:0005886|GO:0015628|GO:0015627
PubMed:  30767847|1365398

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 4-54 5.19e-12

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


:

Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 58.39  E-value: 5.19e-12
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544848476   4 RSTKGNRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQ 54
Cdd:COG2165    3 LRRRRRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLR 53
 
Name Accession Description Interval E-value
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 4-54 5.19e-12

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 58.39  E-value: 5.19e-12
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544848476   4 RSTKGNRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQ 54
Cdd:COG2165    3 LRRRRRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLR 53
PRK10574 PRK10574
putative major pilin subunit; Provisional
 9-35 4.00e-06

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 43.87  E-value: 4.00e-06
                         10        20
                 ....*....|....*....|....*..
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLP 35
Cdd:PRK10574   2 DKQRGFTLIELMVVIAIIAILSAIGIP 28
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 11-42 2.91e-05

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 40.19  E-value: 2.91e-05
                         10        20        30
                 ....*....|....*....|....*....|..
gi 544848476  11 QKGFTLIELAIVLGVIAILTAFFLPGMLKARE 42
Cdd:NF040999   1 VKGFTLIEMLIVLLIISVLLLLFVPNLSKQKE 32
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 9-34 4.78e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 38.51  E-value: 4.78e-05
                          10        20
                  ....*....|....*....|....*.
gi 544848476    9 NRQKGFTLIELAIVLGVIAILTAFFL 34
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 10-53 8.10e-05

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 40.24  E-value: 8.10e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 544848476   10 RQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQL 53
Cdd:TIGR01708   2 RQSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRL 45
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
9-56 1.09e-03

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.02  E-value: 1.09e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLPgmlkaredsklstAITQLQKD 56
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSLLLP-------------SLTQIQKE 35
 
Name Accession Description Interval E-value
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 4-54 5.19e-12

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 58.39  E-value: 5.19e-12
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544848476   4 RSTKGNRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQ 54
Cdd:COG2165    3 LRRRRRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLR 53
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
10-57 8.00e-11

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 54.47  E-value: 8.00e-11
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 544848476  10 RQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQKDF 57
Cdd:COG4970    7 RQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAAL 54
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
4-54 1.02e-10

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 55.85  E-value: 1.02e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544848476   4 RSTKGNRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQ 54
Cdd:COG4968    2 KKRMRRRQRGFTLIELMIVVAIIGILAAIAIPSYQDYVERARRAEAKAALL 52
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
1-45 4.07e-09

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 51.08  E-value: 4.07e-09
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 544848476   1 MIERSTKGNRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSK 45
Cdd:COG4537    1 MKKKKRKLKKEKGFTLIEMLIVLLIISILLLIAVPNLTKQRETAQ 45
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 9-64 6.43e-07

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 45.39  E-value: 6.43e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544848476   9 NRQKGFTLIEL--AIVLG-VIAILTAFFLPGMLKAREDSK--------LSTAITQLQKDFPGAIARQ 64
Cdd:COG4795    6 RRQRGFTLLELlvALAIFaLLLLAAYRGLDSVLRSRERLEqqaerlqeLQRALALLERDLRQAGPRP 72
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
9-55 8.88e-07

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 45.47  E-value: 8.88e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQK 55
Cdd:COG4969    3 KKQKGFTLIELMIVVAIIGILAAIAIPAYQDYVARARVSEALALASP 49
PRK10574 PRK10574
putative major pilin subunit; Provisional
 9-35 4.00e-06

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 43.87  E-value: 4.00e-06
                         10        20
                 ....*....|....*....|....*..
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLP 35
Cdd:PRK10574   2 DKQRGFTLIELMVVIAIIAILSAIGIP 28
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 11-42 2.91e-05

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 40.19  E-value: 2.91e-05
                         10        20        30
                 ....*....|....*....|....*....|..
gi 544848476  11 QKGFTLIELAIVLGVIAILTAFFLPGMLKARE 42
Cdd:NF040999   1 VKGFTLIEMLIVLLIISVLLLLFVPNLSKQKE 32
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 9-34 4.78e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 38.51  E-value: 4.78e-05
                          10        20
                  ....*....|....*....|....*.
gi 544848476    9 NRQKGFTLIELAIVLGVIAILTAFFL 34
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 10-53 8.10e-05

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 40.24  E-value: 8.10e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 544848476   10 RQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQL 53
Cdd:TIGR01708   2 RQSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRL 45
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 11-34 3.55e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 36.13  E-value: 3.55e-04
                          10        20
                  ....*....|....*....|....
gi 544848476   11 QKGFTLIELAIVLGVIAILTAFFL 34
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
PRK10506 PRK10506
prepilin peptidase-dependent protein;
9-56 8.87e-04

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 37.67  E-value: 8.87e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKL-STA------ITQLQKD 56
Cdd:PRK10506   6 KKQRGYTLIELLVVMTIVSILSAWGLYGWQRWQQRQRLwQTAqqlldfLLRLQED 60
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
9-56 1.09e-03

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.02  E-value: 1.09e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 544848476   9 NRQKGFTLIELAIVLGVIAILTAFFLPgmlkaredsklstAITQLQKD 56
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSLLLP-------------SLTQIQKE 35
typeII_sec_gspG TIGR01710
type II secretion system protein G; This model represents GspG, protein G of the main terminal ...
 12-54 1.54e-03

type II secretion system protein G; This model represents GspG, protein G of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130771 [Multi-domain]  Cd Length: 134  Bit Score: 36.64  E-value: 1.54e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 544848476   12 KGFTLIELAIVLGVIAILTAFFLPGMLKAREDSKLSTAITQLQ 54
Cdd:TIGR01710   1 RGFTLLEIMVVLVILGLLAALVAPKLFSQADKAKAQVAKAQIK 43
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
9-54 8.84e-03

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 34.77  E-value: 8.84e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 544848476   9 NRQKGFTLIEL--AIVLGVIAILTAFFLpgMLKAREDSKLSTAITQLQ 54
Cdd:COG4966    2 RRQRGFTLVELmvALAIGLIVLAAVLQL--FLSSRRSYRTQEALARLQ 47
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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