|
Name |
Accession |
Description |
Interval |
E-value |
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
11-352 |
0e+00 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 611.74 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 11 CDCFYAAIEMRDDPSLAQKPLAVGGSADRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASKEIQL 90
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAVGGSPGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 91 IFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGLFVITPDQ 170
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTDNKLCIGSATLIAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 171 VEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSRRQSISVENT 250
Cdd:PRK02406 161 VDAFLATLPVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHFGKFGRRLYERARGIDERPVKPDRERKSVGVERT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 251 FDVDLPDLSSCLAKLPELMETLAGRMARIDSSYRAGKPFVKVKFHDFTQTTLEQAGAGRDLESYQQLMTQAFNR--GGKP 328
Cdd:PRK02406 241 FAEDLYDLEACLAELPRLAEKLERRLERAKPDKRIKTVGVKLKFADFQQTTKEHTADPLDKADLIELLAQALLRrlGGRG 320
|
330 340
....*....|....*....|....
gi 520818450 329 VRLLGIGVRLLDLSGGNeQLEFSW 352
Cdd:PRK02406 321 VRLLGVGVTLLEPQLER-QLLLDL 343
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
7-339 |
3.58e-169 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 474.32 E-value: 3.58e-169
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 7 IHVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRrGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASK 86
Cdd:cd03586 1 IHIDMDAFYASVEQRDNPELKGKPVAVGGSSDR-GVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 87 EIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGLFVI 166
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVI 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 167 TPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSRRQSIS 246
Cdd:cd03586 160 PPEDVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDRERKSIG 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 247 VENTFDVDLPDLSSCLAKLPELMETLAGRMARidSSYRAGKPFVKVKFHDFTQTTLEQA--GAGRDLESYQQLMTQAFNR 324
Cdd:cd03586 240 VERTFSEDLTDPEELLEELLELAEELAERLRK--RGLKGRTVTVKLKYADFSTRTRSRTlpEPTDDAEDIYELALELLEE 317
|
330
....*....|....*..
gi 520818450 325 --GGKPVRLLGIGVRLL 339
Cdd:cd03586 318 llDGRPIRLLGVRLSGL 334
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
4-336 |
2.10e-151 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 429.18 E-value: 2.10e-151
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 4 RKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSAdRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKE 83
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVGGDN-NRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 84 ASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGL 163
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFGSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 164 FVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSRRQ 243
Cdd:COG0389 160 TVIPPGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVEPRRPRK 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 244 SISVENTFDVDLPDLSSCLAKLPELMETLAGRMARidSSYRAGKPFVKVKFHDFTQTTLEQ--AGAGRDLESYQQLMTQA 321
Cdd:COG0389 240 SIGVERTFGEDLTDLEELEAALRRLAERLAERLRR--QGLGARTVTVKLRTSDFRTTTRSRtlPEPTDDTAELLRAAREL 317
|
330
....*....|....*...
gi 520818450 322 FNR---GGKPVRLLGIGV 336
Cdd:COG0389 318 LERiyrPGRPVRLLGVRL 335
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
6-346 |
2.62e-102 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 307.63 E-value: 2.62e-102
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 6 IIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSadRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEAS 85
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPVIIGGG--KRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKPDMEKYVRVG 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 86 KEIQLIFRDYTDLIEPLSLDEAYLDVSDSPH-FGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGLF 164
Cdd:PRK02794 116 REVRAMMQALTPLVEPLSIDEAFLDLSGTERlHGAPPAVVLARFARRVEREIGITVSVGLSYNKFLAKIASDLDKPRGFS 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 165 VITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSRRQS 244
Cdd:PRK02794 196 VIGRAEALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRLARGIDDRKVSPDREAKS 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 245 ISVENTFDVDLPDLSSCLAKLPELMETLAGRMARIDSsyrAGKPFV-KVKFHDFTQTTLEQAG------AGRDLESYQQL 317
Cdd:PRK02794 276 VSAETTFETDLSDFEDLEPILWRLSEKVSRRLKAAGL---AGRTVTlKLKTADFRLRTRRRTLedptqlADRIFRTAREL 352
|
330 340
....*....|....*....|....*....
gi 520818450 318 MTQAfnRGGKPVRLLGIGVRllDLSGGNE 346
Cdd:PRK02794 353 LEKE--TDGTAFRLIGIGVS--DLSPADE 377
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
4-350 |
1.71e-95 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 289.62 E-value: 1.71e-95
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 4 RKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSA-DRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYK 82
Cdd:PRK01810 5 RVIFHVDMNSFFASVEIAYDPSLQGKPLAVAGNEkERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRRPNFDRYR 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 83 EASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHfGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNG 162
Cdd:PRK01810 85 EASRQMFQILSEFTPLVQPVSIDEGYLDITDCYA-LGSPLEIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 163 LFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSRR 242
Cdd:PRK01810 164 ITVLRKRDVPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGIDDRPVDPEAIY 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 243 QSISVEN--TFDVDLPDLSSCLAKLPELMETLAGRMARidSSYRAGKPFVKVKFHDFTQTTLEQAGAG-----RDL-ESY 314
Cdd:PRK01810 244 QFKSVGNstTLSHDMDEEKELLDVLRRLSKSVSKRLQK--KTVVSYNVQIMIRYHDRRTITRSKTLKNpiwekRDIfQAA 321
|
330 340 350
....*....|....*....|....*....|....*..
gi 520818450 315 QQLMTQAFNrgGKPVRLLGI-GVRLLDLSGGNEQLEF 350
Cdd:PRK01810 322 SRLFKQHWN--GDPVRLLGVtATDLEWKTEAVKQLDL 356
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
2-350 |
5.37e-84 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 258.11 E-value: 5.37e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 2 TQRKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRrGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAY 81
Cdd:PRK14133 1 MDRVIIHVDMDAFFASVEQMDNPKLKGKPVIVGGISER-GVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVRHERY 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 82 KEASKEIQLIFRDYTDLIEPLSLDEAYLDVSDsphFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPN 161
Cdd:PRK14133 80 KEVSKNIFKILYEVTPIVEPVSIDEAYLDITN---IKEEPIKIAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKPD 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 162 GLFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQNDSR 241
Cdd:PRK14133 157 GIKIITEDMIPDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYFGKFGVEIYERIRGIDYREVEVSRE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 242 RQSISVENTFDVDLPDLSSCLAKLPELMETLAGRMARIDSSYRAGKpfVKVKFHDFTQTTleqagAGRDLESYQQLMTQA 321
Cdd:PRK14133 237 RKSIGKETTLKKDTKDKEELKKYLKDFSNIISEELKKRNLYGKTVT--VKIKTSDFQTHT-----KSKTLNDYIRDKEEI 309
|
330 340 350
....*....|....*....|....*....|....*..
gi 520818450 322 FNRGGKPVRLLGIG--VRLLDLSGGN------EQLEF 350
Cdd:PRK14133 310 YNVACEILEHINIKepIRLIGLSVSNlsenkiEQLSF 346
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
6-349 |
3.44e-83 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 257.99 E-value: 3.44e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 6 IIHVDCDCFYAAIEMRDDPSLAQKPLAVGGsadrrGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEAS 85
Cdd:PRK03858 6 ILHADLDSFYASVEQRDDPALRGRPVIVGG-----GVVLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPRMSAYSRAS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 86 KEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGLFV 165
Cdd:PRK03858 81 KAVFEVFRDTTPLVEGLSIDEAFLDVGGLRRISGTPVQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 166 ITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFG-SFGERLWSLARGIDDRVVQNDSRRQS 244
Cdd:PRK03858 161 VPPDRELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELPESALVSLLGpAAGRHLHALAHNRDPRRVETGRRRRS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 245 I----------SVENTFDvdlpdlssclAKLPELMETLAGRMARIDssyRAGKPFV-KVKFHDFTQT----TLEQAGAGR 309
Cdd:PRK03858 241 VgaqralgrgpNSPAEVD----------AVVVALVDRVARRMRAAG---RTGRTVVlRLRFDDFTRAtrshTLPRPTAST 307
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 520818450 310 D--LESYQQLMTQAF----NRGgkpVRLLGIGVRLLDlSGGNEQLE 349
Cdd:PRK03858 308 AtlLAAARDLVAAAApliaERG---LTLVGFAVSNLD-DDGAQQLE 349
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
1-336 |
4.00e-83 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 259.48 E-value: 4.00e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 1 MTQRKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRrGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIV-KPRMD 79
Cdd:PRK03348 2 RAQRWVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGR-GVVAGASYEARVFGARSAMPMHQARRLVGNGAVVlPPRFV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 80 AYKEASKEIQLIFRDYTDLIEPLSLDEAYLDVSDspHFGGSA---TRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASD 156
Cdd:PRK03348 81 VYRAASRRVFDTLRELSPVVEQLSFDEAFVEPAE--LAGASAeevEAFAERLRARVREETGLPASVGAGSGKQIAKIASG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 157 WKKPNGLFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFG-SFGERLWSLARGIDDRV 235
Cdd:PRK03348 159 LAKPDGIRVVPPGEERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSEAEVANLLGaTVGPALHRLARGIDDRP 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 236 VQNDSRRQSISVENTFDVDLPDlsscLAKLPELMETLAGRMA-RIDSSYRAGKPF-VKVKFHDFT----QTTLEQA--GA 307
Cdd:PRK03348 239 VAERAEAKQISAESTFAVDLTT----RAQLREAIERIAEHAHrRLLKDGRGARTVtVKLRKSDFStltrSATLPYAtdDA 314
|
330 340
....*....|....*....|....*....
gi 520818450 308 GRDLESYQQLMTQAFNRGgkPVRLLGIGV 336
Cdd:PRK03348 315 AVLAATARRLLLDPDEIG--PIRLVGVGF 341
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
3-280 |
7.56e-79 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 246.84 E-value: 7.56e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 3 QRKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYK 82
Cdd:cd01701 46 QRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVCHGKGPNSEIASCNYEARSYGIKNGMWVGQAKKLCPQLVTLPYDFEAYE 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 83 EASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGG-SATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPN 161
Cdd:cd01701 126 EVSLTFYEILASYTDNIEAVSCDEALIDITSLLEETYeLPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKPD 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 162 GLFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDC--LQLRDWNKLALVREFGS-FGERLWSLARGIDDRVVQN 238
Cdd:cd01701 206 GQYHLSAEKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGDTCggLELRSKTKEKLQKVLGPkTGEKLYDYCRGIDDRPVTG 285
|
250 260 270 280
....*....|....*....|....*....|....*....|..
gi 520818450 239 DSRRQSISVENTFDVDLPDLSSCLAKLPELMETLAGRMARID 280
Cdd:cd01701 286 EKERKSVSAEINYGIRFTNVDDVEQFLQRLSEELSKRLEESN 327
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
8-280 |
1.20e-72 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 229.17 E-value: 1.20e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 8 HVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASKE 87
Cdd:cd00424 2 HIDFDNFFASVEQLARPELKGRPVVVVPFNSDSTCVIACSYEARKYGVKRGMPVREARKMCPNLILVPARLDLYRRLSER 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 88 IQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQL-HITVSAGVAPNKFLAKIASDWKKPNGLFVI 166
Cdd:cd00424 82 LLSELEEVAPLVEVASIDELFLDLTGSARLLGLGSEVALRIKRHIAEQLgGITASIGIASNKLLAKLAAKYAKPDGLTIL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 167 TPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVRE-FGSFGERLWSLARGIDDRVVQNDSRRQSI 245
Cdd:cd00424 162 DPEDLPGFLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAASPDALLALwGGVSGERLWYALRGIDDEPLSPPRPRKSF 241
|
250 260 270
....*....|....*....|....*....|....*
gi 520818450 246 SVENTFDVDLPDLSSCLAKLPELMETLAGRMARID 280
Cdd:cd00424 242 SHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDG 276
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
3-349 |
3.23e-70 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 224.88 E-value: 3.23e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 3 QRKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSADRR-GVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAY 81
Cdd:PRK03103 2 ERVILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRsGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQRY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 82 KEASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASD---WK 158
Cdd:PRK03103 82 IDVSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFGSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMACDnfaKK 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 159 KPNGLFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGIDDRVVQN 238
Cdd:PRK03103 162 NPDGLFTLDKEDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIDYSPVTP 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 239 DS--RRQSISVENTFDVDLPDLSSCLAKLPELMETLAGR------MARI------DSSYRAGKPFVK-VKFHDFTQTTLE 303
Cdd:PRK03103 242 HSldRQKAIGHQMTLPRDYRGFEEIKVVLLELCEEVCRRarakgyMGRTvsvslrGADFDWPTGFSRqMTLPEPTNLAME 321
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 520818450 304 QAGAGRDLesyqqlmtqaFNR--GGKPVRLLGIG--------VRLLDLSGGNEQLE 349
Cdd:PRK03103 322 VYEAACKL----------FHRhwDGKPVRRVGVTlsnlvsddVWQLSLFGDRERKR 367
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
9-156 |
5.82e-67 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 207.43 E-value: 5.82e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 9 VDCDCFYAAIEMRDDPSLAQKPLAVGGSaDRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASKEI 88
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGG-NGRGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRKI 79
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 520818450 89 QLIFRDY-TDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASD 156
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEKLFGAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKLASD 148
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
7-286 |
8.15e-58 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 191.91 E-value: 8.15e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 7 IHVDCDCFYAAIEMRDDPSLAQKPLAVggsaDRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPR-MDAYKEAS 85
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGI----QQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEdLTPFRDMS 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 86 KEIQLIFRDYT--DLIEPLSLDEAYLDVSDSPHFGGSatRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNGL 163
Cdd:cd01703 77 KKVYRLLRSYSwnDRVERLGFDENFMDVTEMRLLVAS--HIAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKPNQQ 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 164 FVITP---DQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDC--LQLRDWN-------------KLALVREFG-SFGERL 224
Cdd:cd01703 155 TTLLPpscADLMDFMDLHDLRKIPGIGYKTAAKLEAHGISSVrdLQEFSNRnrqtvgaapslleLLLMVKEFGeGIGQRI 234
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 520818450 225 WSLARGIDDRVVQNDSRR-QSISVENTF-DVDLPDLSSCLAKLPELMETLAGRMARIDSSYRAG 286
Cdd:cd01703 235 WKLLFGRDTSPVKPASDFpQQISIEDSYkKCSLEEIREARNKIEELLASLLERMKQDLQEVKAG 298
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
4-338 |
2.13e-55 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 184.46 E-value: 2.13e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 4 RKIIHVDCDCFYAAIEMRDDPSLAQKPLAVGGSAD---RRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDA 80
Cdd:PRK03352 5 RWVLHVDLDQFIAAVELLRRPELAGLPVIVGGNGDptePRKVVTCASYEARAFGVRAGMPLRTAARRCPDAVFLPSDPAA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 81 YKEASKEIQLIFRDYTDLIEPLSLDEAYLDV-SDSPhfggsaTRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKK 159
Cdd:PRK03352 85 YDAASEEVMATLRDLGVPVEVWGWDEAFLGVdTDDP------EALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAK 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 160 PNGLFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFG-SFGERLWSLARGIDDRVVQN 238
Cdd:PRK03352 159 PAGVFRLTDANWMAVMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGpTTGPWLLLLARGGGDTEVSA 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 239 DSR-RQSISVENTFDVDLPDLSSCLAKLPELMETLAGRMAriDSSYRAGKPFVKVKFHDF-TQT---TLEQAGAGRD-LE 312
Cdd:PRK03352 239 EPWvPRSRSREVTFPQDLTDRAEVESAVRELARRVLDEVV--AEGRPVTRVAVKVRTATFyTRTkirKLPEPTTDPDvIE 316
|
330 340
....*....|....*....|....*.
gi 520818450 313 SYQQLMTQAFNRgGKPVRLLgiGVRL 338
Cdd:PRK03352 317 AAALDVLDRFEL-DRPVRLL--GVRL 339
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
6-254 |
8.55e-55 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 183.07 E-value: 8.55e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 6 IIHVDCDCFYAAIEMRDDPSLAQKPLAV---GGSADRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYK 82
Cdd:PRK01216 3 ILFVDFDYFFAQVEEVLNPSLKGKPVVVcvySGRFEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEVYQ 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 83 EASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNG 162
Cdd:PRK01216 83 QVSNRIMKLLREYSEKIEIASIDEAYLDISDKVKNYQDAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNG 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 163 LFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFG-SFGERLWSLARG-----IDDRVV 236
Cdd:PRK01216 163 IKVIDDEEVKRFINELDIADIPGIGDITAEKLKKLGVNKLVDTLRIEFDELKGIIGeAKAKYLFSLARNeynepVRARVR 242
|
250
....*....|....*...
gi 520818450 237 QNDSRRQSISvENTFDVD 254
Cdd:PRK01216 243 KSIGRYVTLP-RNTRDLE 259
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
9-335 |
1.10e-50 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 171.96 E-value: 1.10e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 9 VDCDCFYAAIEMRDDPSLAQKPLAVGGSADRRgVIATCNyEARAYGVrsAMSSRhaLKLCPDL------TIVKPRMDAYK 82
Cdd:cd01700 3 VDCNSFYASCERVFRPLLLGRPLVVLSNNDGC-VIARSP-EAKALGI--KMGSP--YFKVPDLlerhgvAVFSSNYALYG 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 83 EASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGgSATRIAQDIRRRVSNQLHITVSAGVAPNKFLAKIASD----WK 158
Cdd:cd01700 77 DMSRRIMSILERFSPDVEVYSIDESFLDLTGSLRFG-DLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDlakkKN 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 159 KPNGLFVITPDQVEDFV-SQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGERLWSLARGID-DRVV 236
Cdd:cd01700 156 PYGGVVDLTDEEVRDKLlKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDcLPLE 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 237 QNDSRRQSISVENTF--DV-DLPDLSSCLAklpELMETLAGRMARIDSSYRA------GKPF-VKVKFHDFTQTTLEQAG 306
Cdd:cd01700 236 EYPPPKKSIGSSRSFgrDVtDLDELKQALA---EYAERAAEKLRRQKSVARTisvfigTSGFsRQPKYYSATNTLPYPTN 312
|
330 340 350
....*....|....*....|....*....|.
gi 520818450 307 AGRDL--ESYQQLmtQAFNRGGKPVRLLGIG 335
Cdd:cd01700 313 DTREIvkAALRLL--YAIYRPGYAYRKAGVM 341
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
8-245 |
2.51e-46 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 161.33 E-value: 2.51e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 8 HVDCDCFYAAIEMRDDPSLAQKPLAVggsADRRGVIATcNYEARAYGVRSAMSSRHALKLCPDLTIV------------- 74
Cdd:cd01702 2 HIDMDAFFAQVEQVRLGLLRNDPVAV---VQWNSIIAV-SYAARAFGVTRFMTIDEAKKKCPDLILAhvatykkgedead 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 75 ----------KPRMDAYKEASKEIQLIFRDYTDLIEPLSLDEAYLDVsdsphfggsATRIAQDIRRRVSNQLHITVSAGV 144
Cdd:cd01702 78 yhenpsparhKVSLDPYRRASRKILNILKRFGDVVEKASIDEAYLDL---------GSRIVEEIRQQVYDELGYTCSAGI 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 145 APNKFLAKIASDWKKPNGLFVITPDQVEDFVSQLRVSKLHG----VGKVTADKLARLGIEDCLQLRDWNKLALVREFGSF 220
Cdd:cd01702 149 AHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLPITSIRGlggkLGEEIIDLLGLPTEGDVAGFRSSESDLQEHFGEKL 228
|
250 260
....*....|....*....|....*
gi 520818450 221 GERLWSLARGIDDRVVQNDSRRQSI 245
Cdd:cd01702 229 GEWLYNLLRGIDHEPVKPRPLPKSM 253
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
7-199 |
2.01e-36 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 138.61 E-value: 2.01e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 7 IHVDCDCFYAAIEMRDDPSLAQKPLAVGGSAdrrgVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASK 86
Cdd:PTZ00205 136 IHLDMDMFYAAVEIKKHPEYAAIPLAIGTMT----MLQTANYVARGRGIRQGMPGFLALKICPNLLILPPDFDAYNEESN 211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 87 EIQLIFRDYTDLIEPLSLDEAYLDVSDS-PHFGGSATR--IAQDIRRRVSNQLHITVSAGVAPNKFLAKIASDWKKPNG- 162
Cdd:PTZ00205 212 TVRRIVAEYDPNYISFGLDELTLEVSAYiERFEGTKTAedVASELRVRVFGETKLTASAGIGPTAALAKIASNINKPNGq 291
|
170 180 190
....*....|....*....|....*....|....*....
gi 520818450 163 --LFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGI 199
Cdd:PTZ00205 292 hdLNLHTRGDVMTYVRDLGLRSVPGVGKVTEALLKGLGI 330
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
21-278 |
1.21e-22 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 96.68 E-value: 1.21e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 21 RDDPSLAQKPLAVGgSADRRGVIATCNYEARAYGVRSAMSSRHALKLCPDLTIVKPRMDAYKEASKEIQLIFRDYTDLIE 100
Cdd:cd03468 15 RNRPADDEAPLAVV-ERKKAGRILACNAAARAAGVRPGMPLAEALALCPNLQVVEYDPEADARALQELALWLLRFTPLVA 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 101 PLSLDEAYLDVSDSPHFGGSATRIAQDIRRRVsNQLHITVSAGVAPNKFLAKIASDWKKPNGLFVITPDQVEDFVSQLRV 180
Cdd:cd03468 94 LDGPDGLLLDVTGCLHLFGGEDALAASLRAAL-ATLGLSARAGIADTPGAAWLLARAGGGRGVLRREALAAALVLLAPLP 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 181 SKLHGVGKVTADKLARLG---IEDCLQL-RDwnklALVREFGSFGERLWSLARGIDDRVVQNDSRRQS--ISVENTFDVD 254
Cdd:cd03468 173 VAALRLPPETVELLARLGlrtLGDLAALpRA----ELARRFGLALLLRLDQAYGRDPEPLLFSPPPPAfdFRLELQLEEP 248
|
250 260
....*....|....*....|....
gi 520818450 255 LPDLSscLAKLPELMETLAGRMAR 278
Cdd:cd03468 249 IARGL--LFPLRRLLEQLCAFLAL 270
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
8-223 |
5.47e-13 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 69.41 E-value: 5.47e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 8 HVDCDCFYAAIEMRDDPSLAQKPLAVGGSADrrGVIATCNYEARAYGVRS-----------------AMSSRHALklcpd 70
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVLSNND--GCVIARSAEAKALGIKMgdpwfkqkdlfrrcgvvCFSSNYEL----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 71 ltivkprmdaYKEASKEIQLIFRDYTDLIEPLSLDEAYLDVSDSPHFGgSATRIAQDIRRRVSNQLHITVSAGVAPNKFL 150
Cdd:PRK03609 77 ----------YADMSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCR-DLTDFGREIRATVLQRTHLTVGVGIAQTKTL 145
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 520818450 151 AKIAS----DWKKPNG--LFVITPDQVEDFVSQLRVSKLHGVGKVTADKLARLGIEDCLQLRDWNKLALVREFGSFGER 223
Cdd:PRK03609 146 AKLANhaakKWQRQTGgvVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLER 224
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
242-340 |
1.39e-11 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 60.27 E-value: 1.39e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 520818450 242 RQSISVENTFDVDLPDLSSCLAKLPELMETLAGRMARIDSsyRAGKPFVKVKFHDFTQTTLEQ--AGAGRDLESYQQLMT 319
Cdd:pfam11799 1 RKSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGL--VARTVTVKIRYSDFRTITRSVtlPSPTDDTDEIYRAAL 78
|
90 100
....*....|....*....|...
gi 520818450 320 QAFNR--GGKPVRLLGIGVRLLD 340
Cdd:pfam11799 79 RLLRRlyRGRPVRLLGVSLSNLV 101
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
168-199 |
9.02e-06 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 42.00 E-value: 9.02e-06
10 20 30
....*....|....*....|....*....|..
gi 520818450 168 PDQVEDFVSQLRVSKLHGVGKVTADKLARLGI 199
Cdd:pfam11798 1 PDDVPEFLWPLPISKIPGIGKKLAEKLKALGI 32
|
|
| |
