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Conserved domains on  [gi|519018225|ref|WP_020174100|]
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HigA family addiction module antitoxin [Methyloferula stellata]

Protein Classification

helix-turn-helix transcriptional regulator( domain architecture ID 10006927)

helix-turn-helix (HTH) transcriptional regulator containing a XRE (Xenobiotic Response Element) family HTH domain, similar to Proteus vulgaris antitoxin HigA that binds to its own promoter and regulates transcription of the higB/higA operon and functions as the antitoxin component of a type II toxin-antitoxin (TA) system that counteracts the effect of the HigB toxin

CATH:  1.10.10.10
Gene Ontology:  GO:0003700|GO:0003677|GO:0006355
PubMed:  10473770|15808743
SCOP:  4000362

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
VapI COG3093
Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense ...
 5-82 2.98e-24

Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense mechanisms];


:

Pssm-ID: 442327 [Multi-domain]  Cd Length: 87  Bit Score: 87.56  E-value: 2.98e-24
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 519018225  5 PKHPGRILQDQYLSDLGMNGSQLAAALKVSGDLISTILRGQAPVDADLSIRLGLYFSKEPNYWLNLQQAFDLAKAQAE 82
Cdd:COG3093   6 PIHPGEILREEFLEPLGLSQTELAKALGVSRQRISEILNGKRAITADTALRLARAFGTSAEFWLNLQAAYDLWLARQK 83
 
Name Accession Description Interval E-value
VapI COG3093
Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense ...
 5-82 2.98e-24

Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense mechanisms];


Pssm-ID: 442327 [Multi-domain]  Cd Length: 87  Bit Score: 87.56  E-value: 2.98e-24
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 519018225  5 PKHPGRILQDQYLSDLGMNGSQLAAALKVSGDLISTILRGQAPVDADLSIRLGLYFSKEPNYWLNLQQAFDLAKAQAE 82
Cdd:COG3093   6 PIHPGEILREEFLEPLGLSQTELAKALGVSRQRISEILNGKRAITADTALRLARAFGTSAEFWLNLQAAYDLWLARQK 83
antidote_HigA TIGR02607
addiction module antidote protein, HigA family; Members of this family form a distinct clade ...
 5-79 8.30e-23

addiction module antidote protein, HigA family; Members of this family form a distinct clade within the larger family HTH_3 of helix-turn-helix proteins, described by pfam01381. Members of this clade are strictly bacterial and nearly always shorter than 110 amino acids. This family includes the characterized member HigA, without which the killer protein HigB cannot be cloned. The hig (host inhibition of growth) system is noted to be unusual in that killer protein is uncoded by the upstream member of the gene pair. [Regulatory functions, DNA interactions, Regulatory functions, Protein interactions, Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274228 [Multi-domain]  Cd Length: 78  Bit Score: 83.44  E-value: 8.30e-23
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 519018225   5 PKHPGRILQDQYLSDLGMNGSQLAAALKVSGDLISTILRGQAPVDADLSIRLGLYFSKEPNYWLNLQQAFDLAKA 79
Cdd:TIGR02607  2 PAHPGEILLEEFLEPLGLSVRALAKALGVSRSTLSRIVNGRAAITADMALRLAKALGTSPEFWLNLQNAYDLWIA 76
 
Name Accession Description Interval E-value
VapI COG3093
Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense ...
 5-82 2.98e-24

Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense mechanisms];


Pssm-ID: 442327 [Multi-domain]  Cd Length: 87  Bit Score: 87.56  E-value: 2.98e-24
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 519018225  5 PKHPGRILQDQYLSDLGMNGSQLAAALKVSGDLISTILRGQAPVDADLSIRLGLYFSKEPNYWLNLQQAFDLAKAQAE 82
Cdd:COG3093   6 PIHPGEILREEFLEPLGLSQTELAKALGVSRQRISEILNGKRAITADTALRLARAFGTSAEFWLNLQAAYDLWLARQK 83
antidote_HigA TIGR02607
addiction module antidote protein, HigA family; Members of this family form a distinct clade ...
 5-79 8.30e-23

addiction module antidote protein, HigA family; Members of this family form a distinct clade within the larger family HTH_3 of helix-turn-helix proteins, described by pfam01381. Members of this clade are strictly bacterial and nearly always shorter than 110 amino acids. This family includes the characterized member HigA, without which the killer protein HigB cannot be cloned. The hig (host inhibition of growth) system is noted to be unusual in that killer protein is uncoded by the upstream member of the gene pair. [Regulatory functions, DNA interactions, Regulatory functions, Protein interactions, Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274228 [Multi-domain]  Cd Length: 78  Bit Score: 83.44  E-value: 8.30e-23
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 519018225   5 PKHPGRILQDQYLSDLGMNGSQLAAALKVSGDLISTILRGQAPVDADLSIRLGLYFSKEPNYWLNLQQAFDLAKA 79
Cdd:TIGR02607  2 PAHPGEILLEEFLEPLGLSVRALAKALGVSRSTLSRIVNGRAAITADMALRLAKALGTSPEFWLNLQNAYDLWIA 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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