DgsA anti-repressor MtfA is a zinc metallopeptidase that functions as a regulator of the phosphoenolpyruvate (PEP)-dependent:glucose phosphotransferase system (ptsG) by binding and inactivating DgsA (aka Mlc), a repressor of ptsG; belongs to the M90 peptidase family
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily ...
35-242
2.58e-78
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily includes the Mlc Titration Factor A (MtfA; also known as YeeI or DgsA anti-repressor MtfA) which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). It can cleave synthetic substrates of both carboxypeptidases and aminopeptidases, with strongest activity towards the latter. Its biologically relevant substrate has yet to be identified. Although it interacts with the transcription repressor Mlc, it does not cleave it. However, Mlc seems to activate the peptidase activity of MtfA. MtfA is related to the catalytic domain of the anthrax lethal factor which is a zinc-dependent metalloprotease, targeting mitogen-activated protein kinase kinases (MAPKKs), and resulting in apoptosis, as well as the Mop (modulation of pathogenesis) protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.
Pssm-ID: 380912 Cd Length: 208 Bit Score: 235.50 E-value: 2.58e-78
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription ...
4-243
5.05e-74
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription factor A that binds Mlc (make large colonies), itself a repressor of glucose and hence a protein important in regulation of the phosphoenolpyruvate:glucose-phosphotransferase (ptsG) system, the major glucose transporter in E.coli. Mlc is a repressor of ptsG, and MtfA is found to bind and inactivate Mlc with high affinity. The membrane-bound protein EIICBGlc encoded by the ptsG gene is the major glucose transporter in Escherichia coli. MtfA is found to be a glucose-regulated peptidase, whose activity is regulated by binding to Mlc available in the cytoplasm, which in turn has been released from EIICBGlc during times when no glucose is taken up. A physiologically relevant target for this peptidase is not yet known.
Pssm-ID: 428801 Cd Length: 243 Bit Score: 225.86 E-value: 5.05e-74
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily ...
35-242
2.58e-78
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily includes the Mlc Titration Factor A (MtfA; also known as YeeI or DgsA anti-repressor MtfA) which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). It can cleave synthetic substrates of both carboxypeptidases and aminopeptidases, with strongest activity towards the latter. Its biologically relevant substrate has yet to be identified. Although it interacts with the transcription repressor Mlc, it does not cleave it. However, Mlc seems to activate the peptidase activity of MtfA. MtfA is related to the catalytic domain of the anthrax lethal factor which is a zinc-dependent metalloprotease, targeting mitogen-activated protein kinase kinases (MAPKKs), and resulting in apoptosis, as well as the Mop (modulation of pathogenesis) protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.
Pssm-ID: 380912 Cd Length: 208 Bit Score: 235.50 E-value: 2.58e-78
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription ...
4-243
5.05e-74
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription factor A that binds Mlc (make large colonies), itself a repressor of glucose and hence a protein important in regulation of the phosphoenolpyruvate:glucose-phosphotransferase (ptsG) system, the major glucose transporter in E.coli. Mlc is a repressor of ptsG, and MtfA is found to bind and inactivate Mlc with high affinity. The membrane-bound protein EIICBGlc encoded by the ptsG gene is the major glucose transporter in Escherichia coli. MtfA is found to be a glucose-regulated peptidase, whose activity is regulated by binding to Mlc available in the cytoplasm, which in turn has been released from EIICBGlc during times when no glucose is taken up. A physiologically relevant target for this peptidase is not yet known.
Pssm-ID: 428801 Cd Length: 243 Bit Score: 225.86 E-value: 5.05e-74
uncharacterized M90 peptidase family-like proteins; This subfamily contains uncharacterized ...
25-240
2.07e-16
uncharacterized M90 peptidase family-like proteins; This subfamily contains uncharacterized M90 peptidase-like domains, similar to the Mlc Titration Factor A (MtfA) peptidase from Escherichia coli, also known as the YeeI gene product, which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). E. coli MtfA has been shown to have aminopeptidase activity with the presence of a single zinc ion in the active site ligated by two histidines in an HEXXH motif. MtfA is related to the catalytic domain of the anthrax lethal factor and the Mop protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.
Pssm-ID: 380913 Cd Length: 210 Bit Score: 75.61 E-value: 2.07e-16
M90 peptidase is a zinc-metallopeptidase; The M90 peptidase family includes the MtfA (Mlc ...
41-236
5.22e-10
M90 peptidase is a zinc-metallopeptidase; The M90 peptidase family includes the MtfA (Mlc Titration Factor A) peptidase from Escherichia coli, also known as the YeeI gene product, which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). E. coli MtfA has been shown to have aminopeptidase activity with the presence of a single zinc ion in the active site ligated by two histidines in a HEXXH motif. This family also includes uncharacterized proteins similar to MtfA peptidase.
Pssm-ID: 380911 Cd Length: 208 Bit Score: 57.91 E-value: 5.22e-10
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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