NCBI Conserved Domain Search

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350229 Cd Length: 127 Bit Score: 175.75 E-value: 5.61e-58

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd09881    1 LLDTNICIDLLRGRPPSVLERLEELLPEDLAISSITLAELLYGAEKSQRPEKNLAALEAFLSRLEVLPFDEEAAEIYGEI 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIEN 131
Cdd:cd09881   81 RAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTKHFERIPGLKVEN 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350312 Cd Length: 130 Bit Score: 172.68 E-value: 1.07e-56

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   4 YLLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGS 83
Cdd:cd18745    1 YLLDTNTVIALFRGRGPVLRARLRAAAPSDLALSSIVLYELEYGAAKSARPERNRARLEELLSLIEVLPFDEEDARAAAE 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 505587673  84 IRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18745   81 IRADLERAGTPIGPYDTLIAGQALARGATLVTHNVREFSRVPGLKVEDW 129

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];

Pssm-ID: 441096 Cd Length: 128 Bit Score: 154.64 E-value: 1.31e-49

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   4 YLLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSqKSDQNQKTLNEFLGYLNMIPFDSEAASIAGS 83
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAELEAGELAISAITLAELLYGARRS-PDAKRRAALEAFLEAFPVLPFDAEAARRAGE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 505587673  84 IRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:COG1487   80 LRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVEPW 128

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350302 Cd Length: 128 Bit Score: 154.30 E-value: 1.48e-49

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18735    1 LLDTNICIYIRKQRPPSVLERFAQLQPGEVAMSVITYGELLYGAEKSQRRAKALEALERLAELIPVLPLPEEVAEHYGEI 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18735   81 RADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKVENW 128

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350309 Cd Length: 127 Bit Score: 144.99 E-value: 8.24e-46

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIInKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18742    1 LLDTDICIYWL-KGNESVKEKFLEVGNEPLSISVITYAELYYGAYNSQRVEKNLARVERFKELLPVLPLSEAIAETFGKI 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18742   80 KADLRKKGNPIDDFDLLIASTALANNLILVTNNTKHFKRIPGLKIENW 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350310 Cd Length: 127 Bit Score: 138.81 E-value: 1.79e-43

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKpENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFdSEAASIAGSI 84
Cdd:cd18743    1 LLDTNICIFLLRGK-YGVAEKIEEVGLENCFISEITIAELLYGAENSARPEKNRKLVDDFLSLFTVLPI-SDALEEYAKE 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENWI 133
Cdd:cd18743   79 KARLRRQGKLIDDFDLLIGATAIANNLTLVTENVKHFARIPGIKLENWI 127

VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC2, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. Rickettsia felis VapC2 cleaves single-stranded RNA. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350301 Cd Length: 129 Bit Score: 138.78 E-value: 2.02e-43

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDN-IFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGS 83
Cdd:cd18734    1 MLDTNICVYLIKKKPDSYYARLEKLQKNNdIAISSIVLAELQYGVSKSQYKEQNQAALNMFLMKLEVLPFEEKATFYYGE 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 505587673  84 IRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18734   81 IRAELEKSGQVIGGNDLLIAAHALAENATLVTNNIKEFARVPNLILENW 129

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350315 Cd Length: 128 Bit Score: 124.57 E-value: 7.96e-38

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQkSDQNQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18748    1 LLDTNIISDLIRNPRGKVARRIREVGEDAVCTSIIVASELRFGAAKKG-SERLARRVEAVLSRIPVLPLEPPADEHYARI 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18748   80 RADLERQGTPIGPNDLLIAAHALALDLTLVTDNTREFSRVPGLKVENW 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350317 Cd Length: 127 Bit Score: 122.35 E-value: 6.38e-37

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTcIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQK-SQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGS 83
Cdd:cd18750    1 LLDTCT-ISDFVRGNPNTLERLKSTSPSLIAISSITVMEIEYGLRLnPQRAKKLRPILEAFLSSITILPFDEEDAIAAAA 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  84 IRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIEN 131
Cdd:cd18750   80 IRAALKKQGTPIGAYDVLIAGTALAHGLILVTSNTKEFQRVDGLVIEN 127

tRNA(fMet)-specific endonuclease VapC;

Pssm-ID: 184277 Cd Length: 132 Bit Score: 122.44 E-value: 8.47e-37

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   1 MNQYLLDTNTCIYIINKKPENVYQKFKkVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASI 80
Cdd:PRK13725   1 MLKFMLDTNICIFTIKNKPEHVRERFN-LNTGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDTAAATH 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 505587673  81 AGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:PRK13725  80 TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRIEDW 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350319 Cd Length: 130 Bit Score: 111.96 E-value: 7.71e-33

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENV-YQKFKKVSLDNIFISTITEFELKYGvqkSQKSDQNQKTLNEFL----GYLNMIPFDSEAAS 79
Cdd:cd18752    1 LFDTDAISELLKKRPSPPlLERLNSIPREDQFTSAITVGELYYG---AYRSDRRLRLLEELLepviSRVTILPFDRESAR 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 505587673  80 IAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18752   78 VYGRLRAELEKKGTPLGEADLQIAAIALAHNLTLVTGNIRHFERIPGLRVENW 130

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350311 Cd Length: 128 Bit Score: 110.66 E-value: 2.49e-32

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPeNVYQKFKKVSL--DNIFISTITEFELKYGVQKSQKSDQnQKTLNEFLGYLNMIPFDSEAASIAG 82
Cdd:cd18744    1 LLDTDILSALLKGNP-KVVEKFREYLKeyGKITISIITYYEILRGLLAKNATKQ-LEVFEEFCSNNEVLPLDDESADIAA 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 505587673  83 SIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18744   79 EIYADLRKKGTLIDDADILIAATAIANNLVLVTNNTKHFQRIEGLKLENW 128

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350307 Cd Length: 126 Bit Score: 110.34 E-value: 3.59e-32

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18740    1 MLDTNTVSHIIKGRPPAVRERLRAVPMASICISSITEAELLYGLAKRPEAKRLHAAVREFLLRVDVLPWDSSVARTYGDL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 505587673  85 RTRLEKKGEIIGPYDLLIASQAIANDIIIVTnNEKEFKRIKELKIEN 131
Cdd:cd18740   81 RAALEAQGKTLGPLDMMIAAHALALGATLVT-NDRAFRRVPGLAVED 126

VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Caulobacter Crescentus VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350303 Cd Length: 123 Bit Score: 109.19 E-value: 7.42e-32

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQktLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18736    1 LLDTNICIYLLKGQPPALARRVAACEPGEVVISAITLAELAYGVRRSAPPRRAA--LDALLEVIPVLPFDEAAAAAYARI 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  85 RTRLEKKGeiiGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18736   79 RAAFPFRR---ASFDRLIAAHALALGLTLVTNNEADFADIPGLRVENW 123

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350314 Cd Length: 132 Bit Score: 99.15 E-value: 8.21e-28

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKP-ENVYQKFKKVSlDNIFISTITEFELKYGVQK---SQKSDQNQKTLNEFLGYLNMIPFDSEAASI 80
Cdd:cd18747    1 LLDTNILSEPLKPEPnANVLARLERHE-GEIAIAAVVWHELLYGCYRlpeGKRKRILEYYLEDVVSGLPILPYDEAAAEW 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 505587673  81 AGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIENW 132
Cdd:cd18747   80 HAEERARLTAIGKTPPFADGQIAAIAVANNLILVTRNTKDFQGFEGLRLENW 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350321 Cd Length: 128 Bit Score: 96.13 E-value: 1.48e-26

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVyqkfKKV-SLDN----IFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAAS 79
Cdd:cd18754    1 ILDTSFLIDLMRGDEAAI----EKArELESesvpQRVSSPTVFELYVGVGLSAAPEEERERIESVLESKPILPLDGESAR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 505587673  80 IAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIE 130
Cdd:cd18754   77 KAGRIDGQLRREGEPIDPGDAMIAATALVHDEPVLTRNVKHFERIDGLEVE 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350308 Cd Length: 120 Bit Score: 80.23 E-value: 1.79e-20

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVyQKFKKVSLDNIFISTITEFELKYGVQKSQKsdqnQKTLNEFLGYLNMIPFDSEAASIAGSI 84
Cdd:cd18741    1 LLDTDILIDFLRGNPSAA-EKLLELLGGNLAISVITVAELYAGARNKKE----LEKLEKLLSLFEVIPVDEEIAELAGEL 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 505587673  85 RTRLeKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIE 130
Cdd:cd18741   76 RRKY-RKSHGLGLPDALIAATALEHGLTLVTLNVKHFPMIEGLKLP 120

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350318 Cd Length: 133 Bit Score: 76.23 E-value: 1.02e-18

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKV--SLDNIFISTITEFELKYGVQKSQKSDQNQKTLNEfLGYLNMIPFDSEAASIAG 82
Cdd:cd18751    1 LLDTDILSEILKGKNPQVVARARAYlaAFGRYTISVITVMEVVRGLQKVQRAERLQQFLEM-IESAEVLPLDRTAAELAG 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 505587673  83 SIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKE----LKIENW 132
Cdd:cd18751   80 RIYADLERTGQPIGRADPMIAAIALRHGLVLVTGNTAHYQRIQElgypLVLENW 133

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350305 Cd Length: 118 Bit Score: 73.72 E-value: 6.21e-18

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKvSLDNIFISTITEFELkYGVQKSqkSDQNQKTLNEFLGYLNMIPFDSEAASIAgsI 84
Cdd:cd18738    1 LLDTNIIIYYLKGELPESLEELLE-ELNEPAISVITKIEL-LGWPKL--TPEEEEEIEDFLSLAEVIPLDDEIARKT--I 74

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 505587673  85 RTRLEKKgeiIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIEN 131
Cdd:cd18738   75 ELRKQYK---IKLPDAIIAATALVHGLTLVTRNVKDFKKIPGLKLLN 118

PIN domain;

Pssm-ID: 426475 Cd Length: 121 Bit Score: 72.44 E-value: 2.32e-17

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673    4 YLLDTNTCIYIINKKPENVYQKFKKVSLDNIFISTITEFELKYGV--QKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIA 81
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLHEAARELLEAAGELVTSAIVLAELLYGLnrRLGLGKAAELVELLLLLSALEVLPIDAELAEEA 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 505587673   82 GSIRTRLEKkgeiIGPYDLLIASQAIANDIIIVTNNEKeFKRIKEL 127
Cdd:pfam01850  81 AELRLKYGK----LGPNDALIAATAKEHGAKLITFDED-FARVAKL 121

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350313 [Multi-domain] Cd Length: 133 Bit Score: 72.08 E-value: 3.69e-17

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNtciyII----NKKPE-NVYQKFKKVSLDNIFISTITEFELKYGVQKSQKSDQNQKT-LNEFLGYL------NMIP 72
Cdd:cd18746    1 LLDTN----VIselrKPRPDpGVVAWLAAVDEDDLFLSVITIGEIRKGIERLRRRDPARAArLEAWLDGLllrfagRILP 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 505587673  73 FDSEAASIAGSIRTRLEKKgeiIGPYDLLIASQAIANDIIIVTNNEKEFKRI 124
Cdd:cd18746   77 VDAEVARRWGRLHAPAPAP---LPVADALIAATALVHGLTLVTRNVRDFEGT 125

VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350300 Cd Length: 131 Bit Score: 71.86 E-value: 4.28e-17

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIinkkpENVYQKFKKVSL----DNIFISTITEFELKYGV-------QKSQKSDQNQKTLNEFlgylNMIPF 73
Cdd:cd18733    3 IIDTSVFIAA-----ERGRLDLEALQQerpdEDVYISAITASELLHGVhraktpeRRARREAFVEAILEAF----PVLDF 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 505587673  74 DSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEFKRIKELKIEN 131
Cdd:cd18733   74 DLEVARTHARLWAELAKRGITIGAHDLLIAATALAHGYSVLTANVRDFKRIPGLEVEE 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350320 Cd Length: 123 Bit Score: 70.58 E-value: 1.06e-16

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVyqkfKKVS-LDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLN--MIPFDSEAASIA 81
Cdd:cd18753    1 LLDTNAYSALLRGDPAVL----EALArADEIYLPVIVLGELLFGFRKGSREEENRERLEEFLSSPRveVLPIDEETAEIY 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 505587673  82 GSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNeKEFKRIKELKI 129
Cdd:cd18753   77 AEIKSALKKKGTPIPENDIWIAALALEHGLPLVTRD-AHFDKIPGLKL 123

VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Neisseria gonorrhoeae FitB toxin of the FitAB toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350298 Cd Length: 136 Bit Score: 63.21 E-value: 9.08e-14

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   4 YLLDTNTCIYIINKKPE-NVYQKFKKVSLDNIFISTITEFELKYGVQK---SQKSDQNQKTLNEFLG--YLNMI-PFDSE 76
Cdd:cd18731    1 IVLDTNVVSELMRPAPDpAVLAWLDAQPAEDLYLTAITVAELLYGIALlpdGRRRDALEAAAEALLAedFAGRIlPFDAA 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 505587673  77 AASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNEKEF 121
Cdd:cd18731   81 AAEAYAEIVAARRRAGRPISVADAQIAAIARAHGATLATRNVKDF 125

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350299 Cd Length: 124 Bit Score: 62.29 E-value: 1.67e-13

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYqkfkkvslDNIFISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNM--IPFDSEAASIAG 82
Cdd:cd18732    1 LLDTSVVIDLERGDPADLP--------DEVAISAITLAELAFGVLAADTRAERAQRLQRLLQAEDFdpLPFDAEAARAYG 72

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 505587673  83 SIRTRLEKKGEIIGP--YDLLIASQAIANDIIIVTNNEKEFKRIKEL 127
Cdd:cd18732   73 RLAAAVRAAGRKPRPraNDLLIAATAIAHGLPLLTRNPDDFAGLDGL 119

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350306 Cd Length: 124 Bit Score: 55.80 E-value: 5.15e-11

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  34 IFISTITEFELKYGVQKSQKSDqNQKTLNEFLGYLNMIPFDSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIII 113
Cdd:cd18739   29 LVTPTIALYEVYVGAVRSAGET-DLDGVREDLDWVEPLDFDEEVAREAAQIQAELLEAGEPINPPDLMIAATARSLGATL 107

                         90
                 ....*....|....*...
gi 505587673 114 VTNNEkEFKRIKELKIEN 131
Cdd:cd18739  108 VTRDG-DFERIDGLEVEN 124

VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin operons, Pyrococcus horikoshii protein PH0500, and other similar bacterial and archaeal homologs; PIN (PilT N terminus) domain-containing proteins of prokaryotic toxin/antitoxin (TA) operons, such as, Mycobacterium tuberculosis VapC of the VapBC (virulence associated proteins) TA operon, and Neisseria gonorrhoeae FitB of the FitAB (fast intracellular trafficking) TA operon, as well as, the archaeal Pyrococcus horikoshii protein PH0500 are included in this family. Toxins of TA operons are believed to be involved in growth inhibition by regulating translation and are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the complex activates the ribonuclease activity of the toxin. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The M. tuberculosis VapC-5 is proposed to be both an endoribonuclease and an exoribonuclease that can act on free RNA in a similar manner to the endo and exonuclease Flap endonuclease-1 (FEN1). VapC-like toxins are structural homologs of FEN1-like PIN domains, but lack the extensive arch/clamp region and the H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region, seen in FEN1-like PIN domains. PIN domains within this group typically contain three or four conserved acidic residues that cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. These putative active site residues are thought to bind Mg2+ and/or Mn2+ ions and be essential for single-stranded ribonuclease activity. VapC-like PIN domains are single domain proteins that form dimers and dimerization configures the active sites in a groove along the long-axis of the structure.

Pssm-ID: 350223 Cd Length: 130 Bit Score: 50.65 E-value: 5.73e-09

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVyqkfKKVSLDNI-----FISTITEFELKYGVQKSQKSDQNQKTLNEFL---GYLNMIPFDSE 76
Cdd:cd09875    1 LLDTSVLIWLLDNDPKSH----LRSLADRIergkvYVSIVSRWELAIGVNINKLRLNYAFADLENLladLAIPVLPLTPR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 505587673  77 AASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTNNeKEFKRIKELKIEN 131
Cdd:cd09875   77 IADEYLEACRNLPLRGRHRSPFDRMIAAQALQHGFTVAHRD-EDFKAINAVNLEW 130

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350316 Cd Length: 126 Bit Score: 49.16 E-value: 1.79e-08

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  36 ISTITEFELKYGVQKSQKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVT 115
Cdd:cd18749   31 ISFVTVAELYFWAEKRGWGEKRLQQLEAWLSRYVVLPYDEEIARTWGRLRAEAQRRGRPRPTNDAWIAATALTHGLPLAT 110

                         90
                 ....*....|....
gi 505587673 116 NNEKEFKRIKELKI 129
Cdd:cd18749  111 HNVKDFEDFPGLQL 124

VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, and VapC21. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350230 Cd Length: 128 Bit Score: 47.20 E-value: 1.06e-07

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  34 IFISTITEFELKYGVqKSQKSDQNQKtlnEFLGYLNMIPFDSEAASIAGSIRTRLEKKG-EIIGPYDLLIASQAIANDII 112
Cdd:cd09882   32 VALTGIILTEVLQGI-RDEKEFQRVK---EYLLALPILPLTGETYIAAARLYRRLRRKGvTVRSTIDCLIAATAIENGLT 107

                         90       100
                 ....*....|....*....|..
gi 505587673 113 IVTnNEKEFKRIKE---LKIEN 131
Cdd:cd09882  108 LLH-NDRDFEAIAKhtgLKLYR 128

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350326 Cd Length: 126 Bit Score: 46.04 E-value: 2.77e-07

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  32 DNIFISTITEFELKYGVqksqKSDQNQKTLNEFLGYLNMIPFDSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDI 111
Cdd:cd18759   29 DEVCTCGIVLAELLQGA----KSEKELSLLEDFLSALPYLPITDELWIKAGELSFSLRKKGITIPLSDLYIAALAIENNL 104

                         90
                 ....*....|....*
gi 505587673 112 IIVTnNEKEFKRIKE 126
Cdd:cd18759  105 ELFT-LDKHFEIIPK 118

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350328 Cd Length: 127 Bit Score: 44.28 E-value: 1.56e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKFKKVSLDN-IFISTITEFELKYGVqksqKSDQNQKTLNEFLGYLNMIPFDSEAASIAGS 83
Cdd:cd18761    1 LIDTSVWISVLRDKTGRVRQQLETLIGGRdVVLTRFTQLELLQGA----RDEREWQLLSDYLETQDYLEATPSTWSAAAR 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 505587673  84 IRTRLEKKG-EIIGPYDLLIASQAIANDIIIVtNNEKEFKRIKE 126
Cdd:cd18761   77 IYFDLRRQGlTVRSPIDCCIAQLALENDLTLL-HDDRDFETIAQ 119

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350333 Cd Length: 130 Bit Score: 43.19 E-value: 3.40e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  53 KSDQNQKTLNEFLGYLNMIPFDSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIANDIIIVTnNEKEFKRI---KELKI 129
Cdd:cd18766   50 RNDKLFDQLKEHLEAFPLILPTREDHIEAARLRNDCRRKGVQVSAIDALIAQMCISHDLTLFT-TDKDFKHIathSSLKL 128

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350335 Cd Length: 123 Bit Score: 42.79 E-value: 4.66e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  32 DNIFISTITEFELKYGVQKSQKSDQNQKTLN--EFLGYLNMIPFDSEAASIAGSIRTRLEKKGEIIGPYDLLIASQAIAN 109
Cdd:cd18768   23 DEIAVSVVTLAELRAGVLAARDLATRARRLAtlQLAADLEPLPIDDAVAAAWARLRLELRESGRRMRVNDSWIAATALAH 102


                 ....*.
gi 505587673 110 DIIIVT 115
Cdd:cd18768  103 DLPVVT 108

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350331 Cd Length: 129 Bit Score: 38.71 E-value: 1.82e-04

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 505587673  53 KSDQNQKTLNEFLGYLNMIPFDSEAASIAGSIRTR-LEKKGEII-GPYDLLIASQAIANDIIIVtNNEKEFKRI 124
Cdd:cd18764   47 RDDNEFKKIKDLLSALPILELDPYEAAIGAADLYRsLRKKGVTIrKSNDCLIAHYAIKFDLPLV-HNDRDFDLI 119

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350325 [Multi-domain] Cd Length: 127 Bit Score: 37.57 E-value: 5.13e-04

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCI-YIINKKPENVYQKFKKV-SLDNIFISTITEFELKYGVqKSQKsdqNQKTLNEFLGYLNMIPFDSEAASIAG 82
Cdd:cd18758    1 LIDTSVWIeALRPRGSPKVKEKVREAlSEDRVATTGIIILELLRGA-KTEK---EYEELKEDLEALPYLPIEEEVWERAA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 505587673  83 SIRTRLEKKGEIIGPYDLLIASQAIANDIIIVtNNEKEFKRIKE---LKIEN 131
Cdd:cd18758   77 KLGFDLRRKGLRVPLTDLLIAAVALKHNCELL-HADRHFDLIAKhspLKVES 127

PIN domain nuclease, a component of toxin-antitoxin system (PIN domain) [Defense mechanisms];

Pssm-ID: 442958 Cd Length: 128 Bit Score: 35.96 E-value: 1.53e-03

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   1 MNqYLLDTNTCIYIINKkPENVYQKFKKV--SLDN-IFISTITEFEL--KYGVQKSQKSDQNQKTLNEFLGYLNM--IPF 73
Cdd:COG3744    1 MK-LLLDTHALLWWLLG-DERLSPAARELieDPANeLYVSAASLWEIaiKVRLGKLDLPEDLDEWLEELLEELGFelLPI 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 505587673  74 DSEAASIAGSIrtrlekkGEIIG-PYDLLIASQAIANDIIIVTNNEK 119
Cdd:COG3744   79 TPEHALAAAQL-------PLHHRdPFDRLLIAQALVEGLPLVTADRK 118

VapC-like PIN domains of VapC and Smg6 ribonucleases, ribosome assembly factor NOB1, rRNA-processing protein Fcf1, Archaeoglobus fulgidus AF0591 protein, and homologs; PIN (PilT N terminus) domains of such ribonucleases as the toxins of prokaryotic toxin/antitoxin operons FitAB and VapBC, as well as, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1, are included in VapC-like this family. Also included are the PIN domains of the Pyrobaculum aerophilum Pea0151 and Archaeoglobus fulgidus AF0591 proteins and other similar archaeal homologs. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350205 Cd Length: 129 Bit Score: 35.71 E-value: 2.25e-03

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673   5 LLDTNTCIYIINKKPENVYQKF---KKVSLDNIFISTITEFELKYGVQKSQKSDQnQKTLNEFLGYLNMIPFDSEAASIA 81
Cdd:cd09854    1 VLDTNVLIALLSSEPESEAAKEllaLLLGDSELVIPPLVLAELLRLLARERGARR-ALEILELLRALEVVEEEPALAEIA 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 505587673  82 GSIRTRLEKKGeiIGPYDLLIASQAIANDI-IIVTNNeKEFKRIKELKIENWI 133
Cdd:cd09854   80 LEVLALGLERG--LDFGDALILALAKELGGaVLVTND-RDFRRLAKLGLKVIV 129

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350304 Cd Length: 115 Bit Score: 34.82 E-value: 4.24e-03

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505587673  36 ISTITEFELKYGVqksqkSDQNQKTLNEFLGYLNMIPFDSEAASIAGSIRTRLekkgeIIGPYDLLIASQAIANDIIIVT 115
Cdd:cd18737   29 ISVITWMEVLVGA-----PPEEEEATRAFLSSFEIIPLDQAIAERAVALRRQS-----RLKLPDAIILATAQVHGLILVT 98


                 ....*..
gi 505587673 116 NNEKEFK 122
Cdd:cd18737   99 RNTKDFP 105