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Conserved domains on  [gi|505469220|ref|WP_015656027|]
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glycosyl hydrolase [Streptomyces davaonensis]

Protein Classification

glycoside hydrolase family protein( domain architecture ID 10448168)

glycoside hydrolase family protein similar to Schizosaccharomyces pombe alkali-sensitive linkage protein 1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glyco_hydro_cc pfam11790
Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is ...
 311-527 4.42e-60

Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is conserved in fungi and some Proteobacteria. The pombe member is annotated as being from IPR013781.


:

Pssm-ID: 432075  Cd Length: 231  Bit Score: 197.96  E-value: 4.42e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  311 SGAHWYFNWASSTGPVAEPDGVEYVPMIWGAGSVTDAelGQAAQQG-----SALLGFNEPDRPEQSAMSPEQALDLWPRL 385
Cdd:pfam11790  13 SKISWYYNWGPYPSGDLSSADFEFVPMLWGPKSVDDW--LANVQSAlgngsKYLLGFNEPDLASQSNMSPSDAAQLWIQL 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  386 E----STGLRLGAPAVSH-GGDVAGGWLDRFMqgAAERGLRVDFIPLHWYGGDFgpdaaNQLRGYLKAVHDRYHKPIWLT 460
Cdd:pfam11790  91 IqplrDGGVKLGSPAVAFtNGGEGLSWLDEFF--EACSGCKVDFLAVHWYGGDF-----DGFKSYIGSFHNAYGKPIWVT 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 505469220  461 EYGLIDFSRNppryPSEQEQTGFIESSTDLLEGLDFVERYAWF----TLSTRTAPTGLYDGT-TANASGRAY 527
Cdd:pfam11790 164 EFACQDDSGD----GSQEETKDFMNEVLAWLDSQDYVERYAWFgfrfDGSGVGANNALLDENgNLTELGQWY 231
Thaumatin pfam00314
Thaumatin family;
60-268 2.52e-46

Thaumatin family;


:

Pssm-ID: 459757  Cd Length: 211  Bit Score: 161.20  E-value: 2.52e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220   60 NRTDSRIWVGSSVNAdGSAALTGLPVLDPGQSATITVPEragtgHWRGKFFARQGCggEEGSTFHCAVGDCGpYADHCS- 138
Cdd:pfam00314   1 NNCPFTIWPGILTNA-GHGPGTGGFELDPGQSRTFTVPD-----GWSGRIWGRTGC--SFDGSGSCATGDCG-GGLECNg 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  139 -LGEQPTGLAEFNFDPADALaPWYNVSYVNAVSVPITITPDNVpvpesGECAMAGCAEELLSACPADDLVKDPaTGEPLV 217
Cdd:pfam00314  72 aGGVPPATLAEFTLNGGGGQ-DFYDVSLVDGYNLPMSITPSGG-----GGCPVAGCAADLNSACPAELQVKSP-GGKVVG 144

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505469220  218 CVNP----NRDA--------------KTAYSDMIAQKCPTAYGWSKQDAEPgnrvVYQCTECTGMTVTF 268
Cdd:pfam00314 145 CKSAclafNTDEycctgayatpetckPTAYSKFFKAACPDAYSYAYDDATS----TFTCPGGADYTITF 209
 
Name Accession Description Interval E-value
Glyco_hydro_cc pfam11790
Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is ...
 311-527 4.42e-60

Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is conserved in fungi and some Proteobacteria. The pombe member is annotated as being from IPR013781.


Pssm-ID: 432075  Cd Length: 231  Bit Score: 197.96  E-value: 4.42e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  311 SGAHWYFNWASSTGPVAEPDGVEYVPMIWGAGSVTDAelGQAAQQG-----SALLGFNEPDRPEQSAMSPEQALDLWPRL 385
Cdd:pfam11790  13 SKISWYYNWGPYPSGDLSSADFEFVPMLWGPKSVDDW--LANVQSAlgngsKYLLGFNEPDLASQSNMSPSDAAQLWIQL 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  386 E----STGLRLGAPAVSH-GGDVAGGWLDRFMqgAAERGLRVDFIPLHWYGGDFgpdaaNQLRGYLKAVHDRYHKPIWLT 460
Cdd:pfam11790  91 IqplrDGGVKLGSPAVAFtNGGEGLSWLDEFF--EACSGCKVDFLAVHWYGGDF-----DGFKSYIGSFHNAYGKPIWVT 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 505469220  461 EYGLIDFSRNppryPSEQEQTGFIESSTDLLEGLDFVERYAWF----TLSTRTAPTGLYDGT-TANASGRAY 527
Cdd:pfam11790 164 EFACQDDSGD----GSQEETKDFMNEVLAWLDSQDYVERYAWFgfrfDGSGVGANNALLDENgNLTELGQWY 231
Thaumatin pfam00314
Thaumatin family;
60-268 2.52e-46

Thaumatin family;


Pssm-ID: 459757  Cd Length: 211  Bit Score: 161.20  E-value: 2.52e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220   60 NRTDSRIWVGSSVNAdGSAALTGLPVLDPGQSATITVPEragtgHWRGKFFARQGCggEEGSTFHCAVGDCGpYADHCS- 138
Cdd:pfam00314   1 NNCPFTIWPGILTNA-GHGPGTGGFELDPGQSRTFTVPD-----GWSGRIWGRTGC--SFDGSGSCATGDCG-GGLECNg 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  139 -LGEQPTGLAEFNFDPADALaPWYNVSYVNAVSVPITITPDNVpvpesGECAMAGCAEELLSACPADDLVKDPaTGEPLV 217
Cdd:pfam00314  72 aGGVPPATLAEFTLNGGGGQ-DFYDVSLVDGYNLPMSITPSGG-----GGCPVAGCAADLNSACPAELQVKSP-GGKVVG 144

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505469220  218 CVNP----NRDA--------------KTAYSDMIAQKCPTAYGWSKQDAEPgnrvVYQCTECTGMTVTF 268
Cdd:pfam00314 145 CKSAclafNTDEycctgayatpetckPTAYSKFFKAACPDAYSYAYDDATS----TFTCPGGADYTITF 209
THN smart00205
Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The ...
 57-268 9.54e-23

Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.


Pssm-ID: 128501 [Multi-domain]  Cd Length: 218  Bit Score: 96.37  E-value: 9.54e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220    57 TFRNRTDSRIWVGSSVNADGSAALTGLPvLDPGQSATITVPeragTGHWRGKFFARQGCGGEEGSTFHCAVGDCGPYADH 136
Cdd:smart00205   2 EFVNNCPYTVWAAALPSGKPQLSGGGFE-LNSGASWQLDAP----PGTKMGRIWARTGCNFDASGRGRCATGDCGGVLQC 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220   137 CSLGEQ-PTGLAEFNFdPADALAPWYNVSYVNAVSVPITITPDNvpvpESGECAMAGCAEELLSACPADDLVKD------ 209
Cdd:smart00205  77 NGWGGRpPATLAEFAL-NQFGGLDFYDVSLVDGFNIPMSFTPTG----GSGDCKGAGCTADLNAQCPAELQVPGggsvva 151

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505469220   210 -----PATGEPLVCV-----NPNRDAKTAYSDMIAQKCPTAYGWSKQDAepgnRVVYQCTECTGMTVTF 268
Cdd:smart00205 152 cnsacTVFGTDQYCCtggqnNPETCPPTNYSRIFKNACPDAYSYAYDDP----TSTFTCTGGTNYKVTF 216
TLP-PA cd09218
allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is ...
 55-269 1.86e-22

allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues.


Pssm-ID: 185757  Cd Length: 219  Bit Score: 95.77  E-value: 1.86e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  55 TVTFRNRTDSRIWVGSSVNADGSAALTGLPVLDPGQSATITVPEragtgHWRGKFFARQGCGGEEGSTFHCAVGDCGPYA 134
Cdd:cd09218    1 TFTIYNKCPFTVWPGILGNAGHPQLGGGGFELAPGQSRTIDAPS-----GWSGRFWGRTGCSFDSSGKGSCATGDCGGGL 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 135 dHC--SLGEQPTGLAEFNFDpADALAPWYNVSYVNAVSVPITITPDNVpvpeSGECAMAGCAEELLSACPADDLVKDP-- 210
Cdd:cd09218   76 -ECngAGGAPPATLAEFTLG-GSGGQDFYDVSLVDGYNLPVSITPQGG----SGGCRTAGCVADLNAVCPAELQVKNSgg 149

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 505469220 211 ----------ATGEPLVC-----VNPNRDAKTAYSDMIAQKCPTAYGWSKQDAEPgnrvVYQCTECTGMTVTFG 269
Cdd:cd09218  150 rvvacksaclAFNTDEYCcrgayGTPETCKPTTYSRLFKNACPQAYSYAYDDPTS----TFTCSSGANYVITFC 219
XynB COG3664
Beta-xylosidase [Carbohydrate transport and metabolism];
390-506 3.89e-06

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442881  Cd Length: 490  Bit Score: 49.53  E-value: 3.89e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 390 LRLGAPAVSHGGDVaggWLDRFMQGAAERGLRVDFIPLHWYGGDFG------PDAANQLRGyLKAVHDR------YHKPI 457
Cdd:COG3664  205 LRVGGPATAGGAAA---WLPDFLEHCAKNGVPVDFVSTHAYGTDPGfdgqlsMNPDAVLRD-VEKVRKLiaasafPNLPL 280

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 505469220 458 WLTEYGL----IDFSRNPPRYPSeqeqtgFIESStdLLEGLDFVERYAWFTLS 506
Cdd:COG3664  281 HITEWNTsytpRDPYHDTAFYAA------YIAKS--LKRAGDLADSMSYWTFS 325
PRK10150 PRK10150
beta-D-glucuronidase; Provisional
 422-497 3.45e-03

beta-D-glucuronidase; Provisional


Pssm-ID: 236657 [Multi-domain]  Cd Length: 604  Bit Score: 39.99  E-value: 3.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 422 VDFIPLH----WY--GGDFGpDAANQLRGYLKAVHDRYHKPIWLTEYG---LIDFSRNPPRYPSEQEQTGFIESSTDLLE 492
Cdd:PRK10150 464 VDVLCLNryygWYvdSGDLE-TAEKVLEKELLAWQEKLHKPIIITEYGadtLAGLHSMYDDMWSEEYQCAFLDMYHRVFD 542


                 ....*
gi 505469220 493 GLDFV 497
Cdd:PRK10150 543 RVPAV 547
 
Name Accession Description Interval E-value
Glyco_hydro_cc pfam11790
Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is ...
 311-527 4.42e-60

Glycosyl hydrolase catalytic core; This family is probably a glycosyl hydrolase, and is conserved in fungi and some Proteobacteria. The pombe member is annotated as being from IPR013781.


Pssm-ID: 432075  Cd Length: 231  Bit Score: 197.96  E-value: 4.42e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  311 SGAHWYFNWASSTGPVAEPDGVEYVPMIWGAGSVTDAelGQAAQQG-----SALLGFNEPDRPEQSAMSPEQALDLWPRL 385
Cdd:pfam11790  13 SKISWYYNWGPYPSGDLSSADFEFVPMLWGPKSVDDW--LANVQSAlgngsKYLLGFNEPDLASQSNMSPSDAAQLWIQL 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  386 E----STGLRLGAPAVSH-GGDVAGGWLDRFMqgAAERGLRVDFIPLHWYGGDFgpdaaNQLRGYLKAVHDRYHKPIWLT 460
Cdd:pfam11790  91 IqplrDGGVKLGSPAVAFtNGGEGLSWLDEFF--EACSGCKVDFLAVHWYGGDF-----DGFKSYIGSFHNAYGKPIWVT 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 505469220  461 EYGLIDFSRNppryPSEQEQTGFIESSTDLLEGLDFVERYAWF----TLSTRTAPTGLYDGT-TANASGRAY 527
Cdd:pfam11790 164 EFACQDDSGD----GSQEETKDFMNEVLAWLDSQDYVERYAWFgfrfDGSGVGANNALLDENgNLTELGQWY 231
Thaumatin pfam00314
Thaumatin family;
60-268 2.52e-46

Thaumatin family;


Pssm-ID: 459757  Cd Length: 211  Bit Score: 161.20  E-value: 2.52e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220   60 NRTDSRIWVGSSVNAdGSAALTGLPVLDPGQSATITVPEragtgHWRGKFFARQGCggEEGSTFHCAVGDCGpYADHCS- 138
Cdd:pfam00314   1 NNCPFTIWPGILTNA-GHGPGTGGFELDPGQSRTFTVPD-----GWSGRIWGRTGC--SFDGSGSCATGDCG-GGLECNg 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  139 -LGEQPTGLAEFNFDPADALaPWYNVSYVNAVSVPITITPDNVpvpesGECAMAGCAEELLSACPADDLVKDPaTGEPLV 217
Cdd:pfam00314  72 aGGVPPATLAEFTLNGGGGQ-DFYDVSLVDGYNLPMSITPSGG-----GGCPVAGCAADLNSACPAELQVKSP-GGKVVG 144

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505469220  218 CVNP----NRDA--------------KTAYSDMIAQKCPTAYGWSKQDAEPgnrvVYQCTECTGMTVTF 268
Cdd:pfam00314 145 CKSAclafNTDEycctgayatpetckPTAYSKFFKAACPDAYSYAYDDATS----TFTCPGGADYTITF 209
THN smart00205
Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The ...
 57-268 9.54e-23

Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.


Pssm-ID: 128501 [Multi-domain]  Cd Length: 218  Bit Score: 96.37  E-value: 9.54e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220    57 TFRNRTDSRIWVGSSVNADGSAALTGLPvLDPGQSATITVPeragTGHWRGKFFARQGCGGEEGSTFHCAVGDCGPYADH 136
Cdd:smart00205   2 EFVNNCPYTVWAAALPSGKPQLSGGGFE-LNSGASWQLDAP----PGTKMGRIWARTGCNFDASGRGRCATGDCGGVLQC 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220   137 CSLGEQ-PTGLAEFNFdPADALAPWYNVSYVNAVSVPITITPDNvpvpESGECAMAGCAEELLSACPADDLVKD------ 209
Cdd:smart00205  77 NGWGGRpPATLAEFAL-NQFGGLDFYDVSLVDGFNIPMSFTPTG----GSGDCKGAGCTADLNAQCPAELQVPGggsvva 151

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505469220   210 -----PATGEPLVCV-----NPNRDAKTAYSDMIAQKCPTAYGWSKQDAepgnRVVYQCTECTGMTVTF 268
Cdd:smart00205 152 cnsacTVFGTDQYCCtggqnNPETCPPTNYSRIFKNACPDAYSYAYDDP----TSTFTCTGGTNYKVTF 216
TLP-PA cd09218
allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is ...
 55-269 1.86e-22

allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues.


Pssm-ID: 185757  Cd Length: 219  Bit Score: 95.77  E-value: 1.86e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  55 TVTFRNRTDSRIWVGSSVNADGSAALTGLPVLDPGQSATITVPEragtgHWRGKFFARQGCGGEEGSTFHCAVGDCGPYA 134
Cdd:cd09218    1 TFTIYNKCPFTVWPGILGNAGHPQLGGGGFELAPGQSRTIDAPS-----GWSGRFWGRTGCSFDSSGKGSCATGDCGGGL 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 135 dHC--SLGEQPTGLAEFNFDpADALAPWYNVSYVNAVSVPITITPDNVpvpeSGECAMAGCAEELLSACPADDLVKDP-- 210
Cdd:cd09218   76 -ECngAGGAPPATLAEFTLG-GSGGQDFYDVSLVDGYNLPVSITPQGG----SGGCRTAGCVADLNAVCPAELQVKNSgg 149

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 505469220 211 ----------ATGEPLVC-----VNPNRDAKTAYSDMIAQKCPTAYGWSKQDAEPgnrvVYQCTECTGMTVTFG 269
Cdd:cd09218  150 rvvacksaclAFNTDEYCcrgayGTPETCKPTTYSRLFKNACPQAYSYAYDDPTS----TFTCSSGANYVITFC 219
Thaumatin-like cd09215
the sweet-tasting protein, thaumatin, and thaumatin-like proteins involved in host defense; ...
 57-202 2.13e-13

the sweet-tasting protein, thaumatin, and thaumatin-like proteins involved in host defense; This family is represented by the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii and thaumatin-like proteins (TLPs) involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. TLPs included in this family are such proteins as zeamatin, found in high concentrations in cereal seeds; osmotin, a salt-induced protein in osmotically stressed plants; and PpAZ44, a propylene-induced TLP in abscission of young fruit. Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). Thaumatin and TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Most TLPs contain 16 conserved Cys residues. A deletion within the third domain (domain II) of the Triticum aestivum thaumatin-like xylanase inhibitor is observed, thus, only 10 conserved Cys residues are present within this smaller TLP and similar homologs.


Pssm-ID: 185754  Cd Length: 157  Bit Score: 67.86  E-value: 2.13e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  57 TFRNRTDSRIWVGSSVNADGSAALTGLPvLDPGQSATITVPEragtgHWRGKFFARQGCG-GEEGSTFHCAVGDCGPYAD 135
Cdd:cd09215    2 TITNRCPYTIWPAIFTQVGKGPYTGGFE-LNPGETKSFDVSA-----GWQGRIWARTNCSfNANSGGNACLTGDCNGGLN 75

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 505469220 136 HCSLGEQPTGLAEFNFDPADALApWYNVSYVNAVSVPITITpdnvpvPESGECAMAGCAeellsACP 202
Cdd:cd09215   76 CQGTGGPPATLAEFTLSGGGGLD-YYDISLVDGYNLPMSIT------PQPGECPTPICA-----ACP 130
GH64-TLP-SF cd08961
glycoside hydrolase family 64 (beta-1,3-glucanases which produce specific pentasaccharide ...
 56-177 2.21e-13

glycoside hydrolase family 64 (beta-1,3-glucanases which produce specific pentasaccharide oligomers) and thaumatin-like proteins; This superfamily includes glycoside hydrolases of family 64 (GH64), these are mostly bacterial beta-1,3-glucanases which cleave long-chain polysaccharide beta-1,3-glucans, into specific pentasaccharide oligomers and are implicated in fungal cell wall degradation. Also included in this superfamily are thaumatin, the sweet-tasting protein from the African berry Thaumatococcus daniellii, and thaumatin-like proteins (TLPs) which are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Like GH64s, some TLPs also hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. Several members of the plant TLP family have been reported as food allergens from fruits, and pollen allergens from conifers. Streptomyces matensis laminaripentaose-producing, beta-1,3-glucanase (GH64-LPHase), and TLPs have in common, a core N-terminal barrel domain (domain I) composed of 10 beta-strands, two coming from the C-terminal region of the protein. In TLPs, this core domain is flanked by two shorter domains (domains II and III). Small TLPs, such as Triticum aestivum thaumatin-like xylanase inhibitor, have a deletion in the third domain (domain II). GH64-LPHase has a second C-terminal domain which corresponds positional to, but is much larger than, domain III of TLP. GH64-LPHase and TLPs are described as crescent-fold structures. Critical functional residues, common to GH64-LPHase and TLPs are a Glu and an Asp residue. LPHase has an electronegative, substrate-binding cleft and the afore mentioned conserved Glu and Asp residues are the catalytic residues essential for beta-1,3-glucan cleavage. In TLPs, these residues are two of the four conserved residues which contribute to the strong electronegative character of the cleft which is associated with the antifungal activity of TLPs.


Pssm-ID: 185752  Cd Length: 153  Bit Score: 67.65  E-value: 2.21e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  56 VTFRNRTDSRIWVGSSVNADGSAALTGLPVLDPGQSATITVPERagtghWRGKFFARQGCGGEEGSTFHCAVGDCGPYAD 135
Cdd:cd08961    1 LTITNNCGYQVWIYNLGTELSSAPDASGPGLAPGRSTTIQIPKG-----FSGRIWFRTGCSMDFSGTTGCLTQDPGVVNP 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 505469220 136 HCSLGEQPTGLAEFNFdPADALAPWYNVSYVNAVSVPITITP 177
Cdd:cd08961   76 TDPNRDPPFTLAEFTL-NDFNSGDFIDSSLVDGFNAPMTVGP 116
TLP-F cd09219
thaumatin-like proteins: basidiomycete homologs; This subfamily is represented by Lentinula ...
 56-224 1.63e-08

thaumatin-like proteins: basidiomycete homologs; This subfamily is represented by Lentinula edodes TLG1, a thaumatin-like protein (TLP), as well as, other basidiomycete homologs. In general, TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. TLG1 TLP is involved in lentinan degradation and fruiting body senescence. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited beta-1,3-glucanase activity and demonstrated lentinan degrading activity. TLG1 is proposed to be involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion. TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. TLG1 from Lentinula edodes contains the required acidic amino acids conserved in the appropriate positions to possess an electronegative cleft. TLPs within this subfamily contain 13 conserved Cys residues; the number of total Cys residues in these TLPs varies from 16 in L. edodes TLG1 to 18 in other basidiomycete homologs.


Pssm-ID: 185758  Cd Length: 229  Bit Score: 55.40  E-value: 1.63e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  56 VTFRNRTDSRIWVG--SSVN-ADGSAALTGLPVLDPGQSATITVPEragtgHWR-GKFFARQGCG-GEEGSTFHCAVGDC 130
Cdd:cd09219    1 FTVKNSCSSTIWPAmfTGGNfIDAVPDQATGWEAAAGGQVEFTVPD-----NWTaGRIWARTGCDfSDNPGPFSCLTGGC 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 131 GPYADHCSLGEQPTGLAEFNFDPADAlaPWYNVSYVNAVSVPITITPD-NVPVPEsgecamagCAEELLSACPADDLVKD 209
Cdd:cd09219   76 GGGLTCENSDQPPASLAEFTLIGGKE--DNYDISLVDGFNIPLNITNNiTCPQPQ--------CQVDLNVLCPALLRGPL 145

                        170
                 ....*....|....*.
gi 505469220 210 PATGEPLVCVNP-NRD 224
Cdd:cd09219  146 DQKGVNLGCISPcNRD 161
TLP-P cd09217
thaumatin and allergenic/antifungal thaumatin-like proteins: plant homologs; This subfamily is ...
 85-212 4.29e-08

thaumatin and allergenic/antifungal thaumatin-like proteins: plant homologs; This subfamily is represented by the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii, allergenic/antifungal Thaumatin-like proteins (TLPs), and related plant proteins. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. TLPs in this subfamily include such proteins as zeamatin, found in high concentrations in cereal seeds, and osmotin, a salt-induced protein in osmotically stressed plants. Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). Thaumatin and TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. IgE-binding epitopes of mountain Cedar (Juniperus ashei) allergen Jun a 3, which interact with pooled IgE from patients suffering allergenic response to this allergen, were mainly located on the helical domain II; the best-conserved IgE-binding epitope predicted for TLPs corresponds to this region. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Most TLPs contain 16 conserved Cys residues. A deletion within the third domain (domain II) of the Triticum aestivum thaumatin-like xylanase inhibitor is observed, thus, only 10 conserved Cys residues are present within this smaller TLP and similar homologs.


Pssm-ID: 185756  Cd Length: 151  Bit Score: 52.39  E-value: 4.29e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220  85 VLDPGQSATITVPerAGTGHwrGKFFARQGCGGEEGSTFHCAVGDCGPYADHCSLGEQPTGLAEFNFDPADalAPWYNVS 164
Cdd:cd09217   23 QLNPGQSWTIDVP--AGTAG--GRIWGRTGCSFDASGRGSCQTGDCGGVLSCTGSGKPPATLAEYTLNQSG--QDFYDIS 96

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 505469220 165 YVNAVSVPITITPDNvpvpesGECAMAGCAeellSACP-ADDLVKDPAT 212
Cdd:cd09217   97 LVDGFNVPMDFSPTG------GGCHAIPCA----ANCPdAYSYPKDPTT 135
XynB COG3664
Beta-xylosidase [Carbohydrate transport and metabolism];
390-506 3.89e-06

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442881  Cd Length: 490  Bit Score: 49.53  E-value: 3.89e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 390 LRLGAPAVSHGGDVaggWLDRFMQGAAERGLRVDFIPLHWYGGDFG------PDAANQLRGyLKAVHDR------YHKPI 457
Cdd:COG3664  205 LRVGGPATAGGAAA---WLPDFLEHCAKNGVPVDFVSTHAYGTDPGfdgqlsMNPDAVLRD-VEKVRKLiaasafPNLPL 280

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 505469220 458 WLTEYGL----IDFSRNPPRYPSeqeqtgFIESStdLLEGLDFVERYAWFTLS 506
Cdd:COG3664  281 HITEWNTsytpRDPYHDTAFYAA------YIAKS--LKRAGDLADSMSYWTFS 325
COG3934 COG3934
Endo-1,4-beta-mannosidase [Carbohydrate transport and metabolism];
363-495 2.30e-03

Endo-1,4-beta-mannosidase [Carbohydrate transport and metabolism];


Pssm-ID: 443135 [Multi-domain]  Cd Length: 331  Bit Score: 40.34  E-value: 2.30e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 363 NEPdRPEQSAMSPEQAlDLWPRLESTGLRLGAP--AVSHG--GDVAGGWLDRFMqgAAERGLRVDFIPLH---WYGGDFG 435
Cdd:COG3934  149 NEP-RNFGDPASPEAA-LAWLREMAAAIKSLDPnhLVSSGdeGDYWEVDDHPFV--PAHAAPLIDYLTVHlypFNWGWVD 224

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 436 PDAANQLRGYLKAVHDRYHKPIWLTEYGlidFSRNPPRYpSEQEQTGFIESSTDLLEGLD 495
Cdd:COG3934  225 RPRSTDKAAYLIELARALGKPVVLEEFG---APRDSPQA-SEEDRAEFYRTVLEAALTLA 280
PRK10150 PRK10150
beta-D-glucuronidase; Provisional
 422-497 3.45e-03

beta-D-glucuronidase; Provisional


Pssm-ID: 236657 [Multi-domain]  Cd Length: 604  Bit Score: 39.99  E-value: 3.45e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 422 VDFIPLH----WY--GGDFGpDAANQLRGYLKAVHDRYHKPIWLTEYG---LIDFSRNPPRYPSEQEQTGFIESSTDLLE 492
Cdd:PRK10150 464 VDVLCLNryygWYvdSGDLE-TAEKVLEKELLAWQEKLHKPIIITEYGadtLAGLHSMYDDMWSEEYQCAFLDMYHRVFD 542


                 ....*
gi 505469220 493 GLDFV 497
Cdd:PRK10150 543 RVPAV 547
XynC COG5520
O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis];
362-462 8.86e-03

O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 444271 [Multi-domain]  Cd Length: 445  Bit Score: 38.67  E-value: 8.86e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505469220 362 FNEPDRP---EQSAMSPEQALD-----LWPRLESTGLRLGAPAVSHGGDVAGGWLDRFMQGAAERGLrVDFIPLHWYGGD 433
Cdd:COG5520  201 QNEPLAPqnwPSCLMTAEEQADfiknyLGPALEKAGLDTKILAYDHNRDYKPDYPITILSDPEAAKY-VDGIAFHWYGGD 279

                         90       100       110
                 ....*....|....*....|....*....|
gi 505469220 434 fgPDAanqlrgyLKAVHDRY-HKPIWLTEY 462
Cdd:COG5520  280 --ISA-------LSRVHEAFpDKNLYFTEG 300
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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