Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503389237    9 LDILKDIILNTVPVEQIFLFGSYVYGEQHTDSDLDIYVVMDENTKIREIDAIRLIRKAIRDKKTMPVDVVVRKKNKFNQL 88
Cdd:pfam01909   1 LRKLREILKELFPVAEVVLFGSYARGTALPGSDIDLLVVFPEPVEEERLLKLAKIIKELEELLGLEVDLVTREKIEFPLV 80

                          90
                  ....*....|
gi 503389237   89 KYTPSIERQI 98
Cdd:pfam01909  81 KIDILEERIL 90

Predicted nucleotidyltransferase MJ0435 [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503389237   3 KKIQDELDILKDIILNTVPVEQIFLFGSYVYGEQHTDSDLDIYVVMDENTKIREIDAIRLirkAIRDKKTMPVDVVVRKK 82
Cdd:COG1669    4 EEILEILREVIEELAERYGVSRLGLFGSVARGEAREDSDIDLLVEFDEPTSLFDLFELEE---ELEELLGRKVDLVTLDG 80

                         90       100
                 ....*....|....*....|...
gi 503389237  83 NKfnqlkytPSIERQIAQEGVML 105
Cdd:COG1669   81 LS-------PRLRERILKEGVLL 96

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503389237   6 QDELDILKDIILNTVP-VEQIFLFGSYVYGEQHTDSDLDIYVVMDENTKIREidaIRLIRKAIRDKKTMPVDVVVrkknk 84
Cdd:cd05403    1 EEILEEILEILRELLGgVEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLE---LARLLEELELLLGRPVDLVV----- 72

                         90       100
                 ....*....|....*....|.
gi 503389237  85 FNQLKYTPSIERQIAQEGVML 105
Cdd:cd05403   73 LNALELELLLRILIEGEGIYL 93

Nucleotidyltransferase (NT) domain of poly(A) polymerases and terminal uridylyl transferases; Poly(A) polymerases (PAPs) catalyze mRNA poly(A) tail synthesis, and terminal uridylyl transferases (TUTases) uridylate RNA. PAPs in this subgroup include human PAP alpha, mouse testis-specific cytoplasmic PAP beta, human nuclear PAP gamma, Saccharomyces cerevisiae PAP1, TRF4 and-5, Schizosaccharomyces pombe caffeine-induced death proteins -1, and -14, Caenorhabditis elegans Germ Line Development-2, and Chlamydomonas reinhardtii MUT68. This family also includes human U6 snRNA-specific TUTase1, and Trypanosoma brucei 3'-TUTase-1,-2, and 4. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. For the majority of proteins in this family, these carboxylate residues are conserved.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 503389237   9 LDILKDIILNTVPVEQIFLFGSYVYGEQHTDSDLDIYVVMDENtKIREIDAIRLIRKAIRD 69
Cdd:cd05402    6 LDRLQELIKEWFPGAKLYPFGSYVTGLGLPGSDIDLCLLGPNH-RVDREDFLRKLAKLLKK 65