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Conserved domains on  [gi|503376841|ref|WP_013611502|]
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MULTISPECIES: GNAT family N-acetyltransferase [Odoribacter]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10006425)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate; similar to Escherichia coli uncharacterized protein YjdJ

CATH:  3.40.630.30
EC:  2.3.1.-|2.3.-.-
Gene Ontology:  GO:0008080|GO:0016746
PubMed:  10940244|9175471|15581578|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
3-89 1.52e-29

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


:

Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 100.61  E-value: 1.52e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503376841  3 YNVKHDEKRHRFEYDRNG-LMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVIPNCAFAQAFLL 81
Cdd:COG2388   1 MEITHNEEKGRFELEVDGeLAGELTYRLEGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAAYFE 80


                ....*...
gi 503376841 82 RHTNYRDL 89
Cdd:COG2388  81 RHPEYADL 88
 
Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
3-89 1.52e-29

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 100.61  E-value: 1.52e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503376841  3 YNVKHDEKRHRFEYDRNG-LMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVIPNCAFAQAFLL 81
Cdd:COG2388   1 MEITHNEEKGRFELEVDGeLAGELTYRLEGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAAYFE 80


                ....*...
gi 503376841 82 RHTNYRDL 89
Cdd:COG2388  81 RHPEYADL 88
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 13-89 4.59e-26

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 91.43  E-value: 4.59e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 503376841  13 RFEY--DRNGLMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVIPNCAFAQAFLLRHTNYRDL 89
Cdd:pfam14542  1 RFEIrvDGGAEVAFLTYRRGDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYADL 79
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 20-70 5.47e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 32.25  E-value: 5.47e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 503376841 20 GLMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVI 70
Cdd:cd04301  12 GFASLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
 
Name Accession Description Interval E-value
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
3-89 1.52e-29

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 100.61  E-value: 1.52e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503376841  3 YNVKHDEKRHRFEYDRNG-LMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVIPNCAFAQAFLL 81
Cdd:COG2388   1 MEITHNEEKGRFELEVDGeLAGELTYRLEGGVIIITHTEVPPALRGQGIASALVEAALDDARERGLKVVPLCPFVAAYFE 80


                ....*...
gi 503376841 82 RHTNYRDL 89
Cdd:COG2388  81 RHPEYADL 88
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 13-89 4.59e-26

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 91.43  E-value: 4.59e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 503376841  13 RFEY--DRNGLMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVIPNCAFAQAFLLRHTNYRDL 89
Cdd:pfam14542  1 RFEIrvDGGAEVAFLTYRRGDGVLIITHTEVPPALRGQGIASKLVKAALDDAREEGLKIVPLCSYVAAYLEKHPEYADL 79
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
47-70 5.11e-03

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 33.52  E-value: 5.11e-03
                         10        20
                 ....*....|....*....|....
gi 503376841  47 GMGVGSALMKYALDYARDHHYSVI 70
Cdd:COG3153   81 GQGIGRALMRAALEAARERGARAV 104
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 20-70 5.47e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 32.25  E-value: 5.47e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 503376841 20 GLMAFVEYDLEDGVMDIFHTLVPIPMEGMGVGSALMKYALDYARDHHYSVI 70
Cdd:cd04301  12 GFASLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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