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Conserved domains on  [gi|502852244|ref|WP_013087220|]
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MULTISPECIES: type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB [Enterobacterales]

Protein Classification

type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB( domain architecture ID 10014381)

type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB is part of a large group of proteins involved in conjugative transfer of plasmid DNA, specifically the F-type system

CATH:  3.40.30.10
PubMed:  16321931|15558583|10049365|12074972

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK13728 PRK13728
conjugal transfer protein TrbB; Provisional
 1-188 4.46e-93

conjugal transfer protein TrbB; Provisional


:

Pssm-ID: 237484  Cd Length: 181  Bit Score: 269.28  E-value: 4.46e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   1 MNIKTGLTALLMCLPLLANAGAREELMALEATKTTSADAA--AITASTIPVPAPASLMALPDGRRANMKDYAVVLFMQAH 78
Cdd:PRK13728   1 MSLTKLLLVLLLLMATAVQASTRDEIERLWNPKGMAAQPAqpAADTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244  79 CQYSAKFDPLLKGWADEHSVRVYPYTLDGGGDVSYPTPMiprktdPNSPiaDEIVTFFGNgLPIATPTAFMVNVNTLKAY 158
Cdd:PRK13728  81 CPYCHQFDPVLKQLAQQYGFSVFPYTLDGQGDTAFPEAL------PAPP--DVMQTFFPN-IPVATPTTFLVNVNTLEAL 151

                        170       180       190
                 ....*....|....*....|....*....|
gi 502852244 159 PLTQGVMDIPALESRMASLIQADMDNVDPK 188
Cdd:PRK13728 152 PLLQGATDAAGFMARMDTVLQMYGGKKGAK 181
 
Name Accession Description Interval E-value
PRK13728 PRK13728
conjugal transfer protein TrbB; Provisional
 1-188 4.46e-93

conjugal transfer protein TrbB; Provisional


Pssm-ID: 237484  Cd Length: 181  Bit Score: 269.28  E-value: 4.46e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   1 MNIKTGLTALLMCLPLLANAGAREELMALEATKTTSADAA--AITASTIPVPAPASLMALPDGRRANMKDYAVVLFMQAH 78
Cdd:PRK13728   1 MSLTKLLLVLLLLMATAVQASTRDEIERLWNPKGMAAQPAqpAADTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244  79 CQYSAKFDPLLKGWADEHSVRVYPYTLDGGGDVSYPTPMiprktdPNSPiaDEIVTFFGNgLPIATPTAFMVNVNTLKAY 158
Cdd:PRK13728  81 CPYCHQFDPVLKQLAQQYGFSVFPYTLDGQGDTAFPEAL------PAPP--DVMQTFFPN-IPVATPTTFLVNVNTLEAL 151

                        170       180       190
                 ....*....|....*....|....*....|
gi 502852244 159 PLTQGVMDIPALESRMASLIQADMDNVDPK 188
Cdd:PRK13728 152 PLLQGATDAAGFMARMDTVLQMYGGKKGAK 181
TrbB TIGR02738
type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB; This protein ...
 34-178 2.26e-35

type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB; This protein is part of a large group of proteins involved in conjugative transfer of plasmid DNA, specifically the F-type system. This protein has been predicted to contain a thioredoxin fold, contains a conserved pair of cysteines and has been shown to function as a thiol disulfide isomerase by complementation of an Ecoli DsbA defect. The protein is believed to be involved in pilin assembly. The protein is closely related to TraF (TIGR02739) which is somewhat longer, lacks the cysteine motif and is apparently not functional as a disulfide bond isomerase.


Pssm-ID: 131785  Cd Length: 153  Bit Score: 121.83  E-value: 2.26e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   34 TTSADAAAITASTIPVPAPASLMALPD----GRRANMKDYAVVLFMQAHCQYSAKFDPLLKGWADEHSVRVYPYTLDGGG 109
Cdd:TIGR02738  13 AGLAQASTLDEITNLWAPPQGLTAATDnapqGRHANQDDYALVFFYQSTCPYCHQFAPVLKRFSQQFGLPVYAFSLDGQG 92

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 502852244  110 DVSYPTPMiprktdPNSPiaDEIVTFFGNGLPIATPTAFMVNVNTLKAYPLTQGVMDIPALESRMASLI 178
Cdd:TIGR02738  93 LTGFPDPL------PATP--EVMQTFFPNPRPVVTPATFLVNVNTRKAYPVLQGAVDEAELANRMDEIL 153
TraF pfam13728
F plasmid transfer operon protein; TraF protein undergoes proteolytic processing associated ...
 63-173 4.83e-08

F plasmid transfer operon protein; TraF protein undergoes proteolytic processing associated with export. The 19 amino acids at the amino terminus of the polypeptides appear to constitute a typical membrane leader peptide - not included in this family, while the remainder of the molecule is predicted to be primarily hydrophilic in character. F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer, and they are both localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor.


Pssm-ID: 433436 [Multi-domain]  Cd Length: 224  Bit Score: 51.15  E-value: 4.83e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   63 RANMKDYAVVLFMQAHCQYSAKFDPLLKGWADEHSVRVYPYTLDGGgdvsyPTPMIPRKTdPNSPIADEIvtffgnGLPI 142
Cdd:pfam13728 125 KSLAEEFGLIFFYRGDCPYCEAQAPILQAFADKYGWTVRPVSVDGR-----PLPGFPNYR-VDNGQAARL------GVKR 192

                          90       100       110
                  ....*....|....*....|....*....|.
gi 502852244  143 aTPTAFMVNVNTLKAYPLTQGVMDIPALESR 173
Cdd:pfam13728 193 -TPALFLVNPPSGDVVPVAAGVLSLDELEER 222
 
Name Accession Description Interval E-value
PRK13728 PRK13728
conjugal transfer protein TrbB; Provisional
 1-188 4.46e-93

conjugal transfer protein TrbB; Provisional


Pssm-ID: 237484  Cd Length: 181  Bit Score: 269.28  E-value: 4.46e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   1 MNIKTGLTALLMCLPLLANAGAREELMALEATKTTSADAA--AITASTIPVPAPASLMALPDGRRANMKDYAVVLFMQAH 78
Cdd:PRK13728   1 MSLTKLLLVLLLLMATAVQASTRDEIERLWNPKGMAAQPAqpAADTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGH 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244  79 CQYSAKFDPLLKGWADEHSVRVYPYTLDGGGDVSYPTPMiprktdPNSPiaDEIVTFFGNgLPIATPTAFMVNVNTLKAY 158
Cdd:PRK13728  81 CPYCHQFDPVLKQLAQQYGFSVFPYTLDGQGDTAFPEAL------PAPP--DVMQTFFPN-IPVATPTTFLVNVNTLEAL 151

                        170       180       190
                 ....*....|....*....|....*....|
gi 502852244 159 PLTQGVMDIPALESRMASLIQADMDNVDPK 188
Cdd:PRK13728 152 PLLQGATDAAGFMARMDTVLQMYGGKKGAK 181
TrbB TIGR02738
type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB; This protein ...
 34-178 2.26e-35

type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB; This protein is part of a large group of proteins involved in conjugative transfer of plasmid DNA, specifically the F-type system. This protein has been predicted to contain a thioredoxin fold, contains a conserved pair of cysteines and has been shown to function as a thiol disulfide isomerase by complementation of an Ecoli DsbA defect. The protein is believed to be involved in pilin assembly. The protein is closely related to TraF (TIGR02739) which is somewhat longer, lacks the cysteine motif and is apparently not functional as a disulfide bond isomerase.


Pssm-ID: 131785  Cd Length: 153  Bit Score: 121.83  E-value: 2.26e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   34 TTSADAAAITASTIPVPAPASLMALPD----GRRANMKDYAVVLFMQAHCQYSAKFDPLLKGWADEHSVRVYPYTLDGGG 109
Cdd:TIGR02738  13 AGLAQASTLDEITNLWAPPQGLTAATDnapqGRHANQDDYALVFFYQSTCPYCHQFAPVLKRFSQQFGLPVYAFSLDGQG 92

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 502852244  110 DVSYPTPMiprktdPNSPiaDEIVTFFGNGLPIATPTAFMVNVNTLKAYPLTQGVMDIPALESRMASLI 178
Cdd:TIGR02738  93 LTGFPDPL------PATP--EVMQTFFPNPRPVVTPATFLVNVNTRKAYPVLQGAVDEAELANRMDEIL 153
TraF pfam13728
F plasmid transfer operon protein; TraF protein undergoes proteolytic processing associated ...
 63-173 4.83e-08

F plasmid transfer operon protein; TraF protein undergoes proteolytic processing associated with export. The 19 amino acids at the amino terminus of the polypeptides appear to constitute a typical membrane leader peptide - not included in this family, while the remainder of the molecule is predicted to be primarily hydrophilic in character. F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer, and they are both localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor.


Pssm-ID: 433436 [Multi-domain]  Cd Length: 224  Bit Score: 51.15  E-value: 4.83e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   63 RANMKDYAVVLFMQAHCQYSAKFDPLLKGWADEHSVRVYPYTLDGGgdvsyPTPMIPRKTdPNSPIADEIvtffgnGLPI 142
Cdd:pfam13728 125 KSLAEEFGLIFFYRGDCPYCEAQAPILQAFADKYGWTVRPVSVDGR-----PLPGFPNYR-VDNGQAARL------GVKR 192

                          90       100       110
                  ....*....|....*....|....*....|.
gi 502852244  143 aTPTAFMVNVNTLKAYPLTQGVMDIPALESR 173
Cdd:pfam13728 193 -TPALFLVNPPSGDVVPVAAGVLSLDELEER 222
TraF-like TIGR02740
TraF-like protein; This protein is related to the F-type conjugation system pilus assembly ...
 67-175 9.93e-07

TraF-like protein; This protein is related to the F-type conjugation system pilus assembly proteins TraF (TIGR02739)and TrbB (TIGR02738) both of which exhibit a thioredoxin fold. The protein represented by this model has the same length and architecture as TraF, but lacks the CXXC-motif found in TrbB and believed to be responsible for the disulfide isomerase activity of that protein.


Pssm-ID: 274275  Cd Length: 271  Bit Score: 47.79  E-value: 9.93e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 502852244   67 KDYAVVLFMQAHCQYSAKFDPLLKGWADEHSVRVYPYTLDGGGDVSYPTPMiprktdPNSPIADEivtffgngLPIAT-P 145
Cdd:TIGR02740 166 KKSGLFFFFKSDCPYCHQQAPILQAFEDRYGIEVLPVSVDGGPLPGFPNAR------PDAGQAQQ--------LKIRTvP 231

                          90       100       110
                  ....*....|....*....|....*....|
gi 502852244  146 TAFMVNVNTLKAYPLTQGVMDIPALESRMA 175
Cdd:TIGR02740 232 AVFLADPDPNQFTPIGFGVMSADELVDRIL 261
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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