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Conserved domains on  [gi|501564778|ref|WP_012569238|]
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MULTISPECIES: N-6 DNA methylase [Enterobacteriaceae]

Protein Classification

N-6 DNA methylase family protein( domain architecture ID 1006218)

N-6 DNA methylase family protein might be a component of a type I or type IC restriction system and protect the host DNA from digestion via sequence-specific methylation

CATH:  1.20.1260.30
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:0003677
PubMed:  35205080
SCOP:  4003146

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
N6_Mtase super family cl37510
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 112-231 5.79e-09

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


The actual alignment was detected with superfamily member pfam02384:

Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 55.79  E-value: 5.79e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501564778  112 DIFNELFEDLF--LTGKKGDSFGQFLTPTDISELLTDIVYTtsKDKYKIADSCAGTGSLIFPLIKriFFKEGFEGIQKVE 189
Cdd:pfam02384   3 DLFGDAYEYLLrkFAPNAGKSGGEFFTPREVSKLIVELLDP--KPGESIYDPACGSGGFLIQAEK--FVKEHDGDTNDLS 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 501564778  190 LFYNDKDSFVSQLfiaqILTNMIYHNLDFKSLHVYIGNAITE 231
Cdd:pfam02384  79 IYGQEKNPTTYRL----ARMNMILHGIEYDDFHIRHGDTLTS 116
 
Name Accession Description Interval E-value
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 112-231 5.79e-09

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 55.79  E-value: 5.79e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501564778  112 DIFNELFEDLF--LTGKKGDSFGQFLTPTDISELLTDIVYTtsKDKYKIADSCAGTGSLIFPLIKriFFKEGFEGIQKVE 189
Cdd:pfam02384   3 DLFGDAYEYLLrkFAPNAGKSGGEFFTPREVSKLIVELLDP--KPGESIYDPACGSGGFLIQAEK--FVKEHDGDTNDLS 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 501564778  190 LFYNDKDSFVSQLfiaqILTNMIYHNLDFKSLHVYIGNAITE 231
Cdd:pfam02384  79 IYGQEKNPTTYRL----ARMNMILHGIEYDDFHIRHGDTLTS 116
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
112-231 2.57e-07

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 50.19  E-value: 2.57e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501564778 112 DIFNELFEDL---FLTGKKGDSfGQFLTPTDISELLTDIVytTSKDKYKIADSCAGTGSLIFPLIKRIfFKEGFEGIQKV 188
Cdd:COG0286    1 DVLGDAYEYLlrkFAEESGKKA-GEFYTPREVVRLMVELL--DPKPGETVYDPACGSGGFLVEAAEYL-KEHGGDERKKL 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 501564778 189 ELFYNDKDSFVSQLfiAQIltNMIYHNLDfkSLHVYIGNAITE 231
Cdd:COG0286   77 SLYGQEINPTTYRL--AKM--NLLLHGIG--DPNIELGDTLSN 113
 
Name Accession Description Interval E-value
N6_Mtase pfam02384
N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against ...
 112-231 5.79e-09

N-6 DNA Methylase; Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The R-M system is a complex containing three polypeptides: M (this family), S (pfam01420), and R. This family consists of N-6 adenine-specific DNA methylase EC:2.1.1.72 from Type I and Type IC restriction systems. These methylases have the same sequence specificity as their corresponding restriction enzymes.


Pssm-ID: 426749 [Multi-domain]  Cd Length: 310  Bit Score: 55.79  E-value: 5.79e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501564778  112 DIFNELFEDLF--LTGKKGDSFGQFLTPTDISELLTDIVYTtsKDKYKIADSCAGTGSLIFPLIKriFFKEGFEGIQKVE 189
Cdd:pfam02384   3 DLFGDAYEYLLrkFAPNAGKSGGEFFTPREVSKLIVELLDP--KPGESIYDPACGSGGFLIQAEK--FVKEHDGDTNDLS 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 501564778  190 LFYNDKDSFVSQLfiaqILTNMIYHNLDFKSLHVYIGNAITE 231
Cdd:pfam02384  79 IYGQEKNPTTYRL----ARMNMILHGIEYDDFHIRHGDTLTS 116
HsdM COG0286
Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];
112-231 2.57e-07

Type I restriction-modification system, DNA methylase subunit [Defense mechanisms];


Pssm-ID: 440055 [Multi-domain]  Cd Length: 243  Bit Score: 50.19  E-value: 2.57e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501564778 112 DIFNELFEDL---FLTGKKGDSfGQFLTPTDISELLTDIVytTSKDKYKIADSCAGTGSLIFPLIKRIfFKEGFEGIQKV 188
Cdd:COG0286    1 DVLGDAYEYLlrkFAEESGKKA-GEFYTPREVVRLMVELL--DPKPGETVYDPACGSGGFLVEAAEYL-KEHGGDERKKL 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 501564778 189 ELFYNDKDSFVSQLfiAQIltNMIYHNLDfkSLHVYIGNAITE 231
Cdd:COG0286   77 SLYGQEINPTTYRL--AKM--NLLLHGIG--DPNIELGDTLSN 113
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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