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Conserved domains on  [gi|501073729|ref|WP_012124669|]
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MULTISPECIES: GNAT family N-acetyltransferase [Cronobacter]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 12226222)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 4-142 7.26e-24

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


:

Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 90.50  E-value: 7.26e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   4 YLIEPSDPTHPDAIVLLAALSETLAALTGSsgrqsfnnddvraEGATFLLARNASGTAIGCVACRPLKPGIAEIKRLFAL 83
Cdd:COG0454    1 MSIRKATPEDINFILLIEALDAELKAMEGS-------------LAGAEFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVL 67

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 501073729  84 PD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYEIIDNYGRYVGQE 142
Cdd:COG0454   68 PEyrGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERYVAYVGGE 128
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 4-142 7.26e-24

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 90.50  E-value: 7.26e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   4 YLIEPSDPTHPDAIVLLAALSETLAALTGSsgrqsfnnddvraEGATFLLARNASGTAIGCVACRPLKPGIAEIKRLFAL 83
Cdd:COG0454    1 MSIRKATPEDINFILLIEALDAELKAMEGS-------------LAGAEFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVL 67

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 501073729  84 PD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYEIIDNYGRYVGQE 142
Cdd:COG0454   68 PEyrGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERYVAYVGGE 128
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 42-129 3.52e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 59.45  E-value: 3.52e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   42 DDVRAEGATFLLARNaSGTAIGCVACRPL--KPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTN 117
Cdd:pfam00583  26 DWDEDASEGFFVAEE-DGELVGFASLSIIddEPPVGEIEGLAVAPEyrGKGIGTALLQALLEWARERGCERIFLEVAADN 104

                          90
                  ....*....|..
gi 501073729  118 PRAVEFYLRRGY 129
Cdd:pfam00583 105 LAAIALYEKLGF 116
PRK10146 PRK10146
aminoalkylphosphonate N-acetyltransferase;
74-130 2.99e-06

aminoalkylphosphonate N-acetyltransferase;


Pssm-ID: 182266 [Multi-domain]  Cd Length: 144  Bit Score: 44.52  E-value: 2.99e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 501073729  74 IAEIKRLFALPDNPG--AGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYE 130
Cdd:PRK10146  76 IGEIQELVVMPQARGlnVGSKLLAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYE 134
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-112 3.04e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 3.04e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 501073729  51 FLLARNaSGTAIGCVACRP--LKPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLE 112
Cdd:cd04301    1 FLVAED-DGEIVGFASLSPdgSGGDTAYIGDLAVLPEyrGKGIGSALLEAAEEEARERGAKRLRLE 65
PseH TIGR03585
UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase; Sequences in this ...
 87-139 5.26e-03

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase; Sequences in this family are members of the pfam00583 (GNAT) superfamily of acetyltransferases and are proposed to perform a N-acetylation step in the process of pseudaminic acid biosynthesis in Campylobacter species. This gene is commonly observed in apparent operons with other genes responsible for the biosynthesis of pseudaminic acid and as a component of flagellar and exopolysaccharide biosynthesis loci. Significantly, many genomes containing other components of this pathway lack this gene, indicating that some other N-acetyl transferases may be incolved and/or the step is optional, resulting in a non-acetylated pseudaminic acid variant sugar.


Pssm-ID: 274661 [Multi-domain]  Cd Length: 152  Bit Score: 35.41  E-value: 5.26e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 501073729   87 PGAGSQLLSAAERFA-RDAGYDQAWLETRRTNPRAVEFYLRRGYE---IIDNYGRYV 139
Cdd:TIGR03585  90 PGVGSVLEEAALEYAfEHLGLHKLSLEVLESNNKALKLYEKFGFEregVFRQGGEYY 146
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 4-142 7.26e-24

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 90.50  E-value: 7.26e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   4 YLIEPSDPTHPDAIVLLAALSETLAALTGSsgrqsfnnddvraEGATFLLARNASGTAIGCVACRPLKPGIAEIKRLFAL 83
Cdd:COG0454    1 MSIRKATPEDINFILLIEALDAELKAMEGS-------------LAGAEFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVL 67

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 501073729  84 PD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYEIIDNYGRYVGQE 142
Cdd:COG0454   68 PEyrGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERYVAYVGGE 128
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 39-152 1.94e-18

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 76.18  E-value: 1.94e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  39 FNNDDVRAEGATFLLARnASGTAIGCVACRPLKPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRT 116
Cdd:COG1246   18 IRPYALEEEIGEFWVAE-EDGEIVGCAALHPLDEDLAELRSLAVHPDyrGRGIGRRLLEALLAEARELGLKRLFLLTTSA 96

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 501073729 117 nprAVEFYLRRGYEIIDNY---GRYVGQEDAVCFAKPLQ 152
Cdd:COG1246   97 ---AIHFYEKLGFEEIDKEdlpYAKVWQRDSVVMEKDLE 132
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 63-151 7.87e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 70.84  E-value: 7.87e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  63 GCVACRPL-KPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYEIIDNYGRYv 139
Cdd:COG0456    1 GFALLGLVdGGDEAEIEDLAVDPEyrGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNY- 79

                         90
                 ....*....|..
gi 501073729 140 GQEDAVCFAKPL 151
Cdd:COG0456   80 YGDDALVMEKEL 91
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 42-129 3.52e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 59.45  E-value: 3.52e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   42 DDVRAEGATFLLARNaSGTAIGCVACRPL--KPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTN 117
Cdd:pfam00583  26 DWDEDASEGFFVAEE-DGELVGFASLSIIddEPPVGEIEGLAVAPEyrGKGIGTALLQALLEWARERGCERIFLEVAADN 104

                          90
                  ....*....|..
gi 501073729  118 PRAVEFYLRRGY 129
Cdd:pfam00583 105 LAAIALYEKLGF 116
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
62-139 2.70e-11

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 56.46  E-value: 2.70e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  62 IGCVACRPLKPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYEIIDNYGRYV 139
Cdd:COG3393    3 VAMAGVRAESPGVAEISGVYTHPEyrGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVGEYATVL 82
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
43-132 2.59e-10

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 55.19  E-value: 2.59e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  43 DVRAEGATFLLARNAsGTAIGCVACRPLKPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRTnprA 120
Cdd:COG2153   28 DGKDEDARHLLAYDD-GELVATARLLPPGDGEAKIGRVAVLPEyrGQGLGRALMEAAIEEARERGARRIVLSAQAH---A 103

                         90
                 ....*....|..
gi 501073729 121 VEFYLRRGYEII 132
Cdd:COG2153  104 VGFYEKLGFVPV 115
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
6-151 3.93e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 52.01  E-value: 3.93e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   6 IEPSDPTHPDAIvllaalsETLAALTGSSGRQSFNNDDVRAEGA--TFLLARnASGTAIGCVACRPLK----PGIAEIKR 79
Cdd:COG3153    1 IRPATPEDAEAI-------AALLRAAFGPGREAELVDRLREDPAagLSLVAE-DDGEIVGHVALSPVDidgeGPALLLGP 72

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 501073729  80 LFALPD--NPGAGSQLLSAAERFARDAGYDQAWLEtrrTNPRAVEFYLRRGYEIIDNYGRYVGQEDAVcFAKPL 151
Cdd:COG3153   73 LAVDPEyrGQGIGRALMRAALEAARERGARAVVLL---GDPSLLPFYERFGFRPAGELGLTLGPDEVF-LAKEL 142
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
43-151 1.23e-08

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 51.15  E-value: 1.23e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  43 DVRAEGATFLLARnASGTAIGCVACRPLKP-----GIAEIKrLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLETRR 115
Cdd:COG1247   46 AILAPGRPVLVAE-EDGEVVGFASLGPFRPrpayrGTAEES-IYVDPDarGRGIGRALLEALIERARARGYRRLVAVVLA 123

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 501073729 116 TNPRAVEFYLRRGYEIIDNY---GRYVGQE-DAVCFAKPL 151
Cdd:COG1247  124 DNEASIALYEKLGFEEVGTLpevGFKFGRWlDLVLMQKRL 163
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 47-131 1.17e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 44.37  E-value: 1.17e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729   47 EGATFLLARNAsGTAIGCVACRPLKPGIAEIKRLFA-LPD--NPGAGSQLLSAAERFARDAGYDQAWLETRRtnpRAVEF 123
Cdd:pfam13508   1 PGGRFFVAEDD-GKIVGFAALLPLDDEGALAELRLAvHPEyrGQGIGRALLEAAEAAAKEGGIKLLELETTN---RAAAF 76


                  ....*...
gi 501073729  124 YLRRGYEI 131
Cdd:pfam13508  77 YEKLGFEE 84
PRK10146 PRK10146
aminoalkylphosphonate N-acetyltransferase;
74-130 2.99e-06

aminoalkylphosphonate N-acetyltransferase;


Pssm-ID: 182266 [Multi-domain]  Cd Length: 144  Bit Score: 44.52  E-value: 2.99e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 501073729  74 IAEIKRLFALPDNPG--AGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGYE 130
Cdd:PRK10146  76 IGEIQELVVMPQARGlnVGSKLLAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYE 134
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-112 3.04e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 3.04e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 501073729  51 FLLARNaSGTAIGCVACRP--LKPGIAEIKRLFALPD--NPGAGSQLLSAAERFARDAGYDQAWLE 112
Cdd:cd04301    1 FLVAED-DGEIVGFASLSPdgSGGDTAYIGDLAVLPEyrGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
52-131 7.96e-04

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 37.99  E-value: 7.96e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501073729  52 LLARNASGTAIGCVACRPLKPGIAEIKRLFALPDNP--GAGSQLLSAAERFARDAGYDQAWLETRRTNPRAVEFYLRRGY 129
Cdd:PRK10975 104 LLLRDASGQIQGFVTLRELNDTDARIGLLAVFPGAQgrGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLYIRSGA 183


                 ..
gi 501073729 130 EI 131
Cdd:PRK10975 184 NI 185
PseH TIGR03585
UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase; Sequences in this ...
 87-139 5.26e-03

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase; Sequences in this family are members of the pfam00583 (GNAT) superfamily of acetyltransferases and are proposed to perform a N-acetylation step in the process of pseudaminic acid biosynthesis in Campylobacter species. This gene is commonly observed in apparent operons with other genes responsible for the biosynthesis of pseudaminic acid and as a component of flagellar and exopolysaccharide biosynthesis loci. Significantly, many genomes containing other components of this pathway lack this gene, indicating that some other N-acetyl transferases may be incolved and/or the step is optional, resulting in a non-acetylated pseudaminic acid variant sugar.


Pssm-ID: 274661 [Multi-domain]  Cd Length: 152  Bit Score: 35.41  E-value: 5.26e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 501073729   87 PGAGSQLLSAAERFA-RDAGYDQAWLETRRTNPRAVEFYLRRGYE---IIDNYGRYV 139
Cdd:TIGR03585  90 PGVGSVLEEAALEYAfEHLGLHKLSLEVLESNNKALKLYEKFGFEregVFRQGGEYY 146
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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