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Conserved domains on  [gi|501062371|ref|WP_012113668|]
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hypothetical protein [Xanthobacter sp. V7C-4]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
AlgX_N_like super family cl16774
N-terminal catalytic domain of putative alginate O-acetyltranferase and similar proteins; The ...
57-372 2.14e-33

N-terminal catalytic domain of putative alginate O-acetyltranferase and similar proteins; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. This wider family includes AlgX, AlgJ, AlgV, and a number of uncharacterized families, some of which may have been mis-annotated in sequence databases.


The actual alignment was detected with superfamily member cd14440:

Pssm-ID: 450039 [Multi-domain]  Cd Length: 315  Bit Score: 126.32  E-value: 2.14e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  57 INNTFGFRGAVLTA--HLSYVRAmGDSMTSRVLKGEHGALFLNEDKALEQSIGQlvRPEPLGKLFDMVKLLN---DYMTA 131
Cdd:cd14440    1 FSDHFGFRDQLISAdsALLYSLL-GESSNPRVIIGKDGWLFLGEDYMLEDYCGR--DPLSEEDLRRWVALLErrrDWLAA 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 132 HGGRFVMLVPPNGHTANFELLPAYARRLKKAPTEyDLVAQRMKEAGITFVDMRPIMAEAKKTG-PVHWRYDTHWNLRGAL 210
Cdd:cd14440   78 RGIPFVVVVAPNKHTIYPEHLPSWYPGKSPTRLD-QLLALLLSAAGVGVVDLRPALLEAKATGaPVYYKTDTHWNFYGAY 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 211 LGFNAAMAAAGRPDLEVSEGDALEPPVPSYVGDLGRLSGiRPAEPDLDYpgkaPMQAPRNPVAVPGVMPEVSKTDPFV-- 288
Cdd:cd14440  157 VAYRAIAEALGPGVPALWPLASVEYDESTVGGDLANMLG-LPEALEEDR----LPDESKPPLQARRIDDDTATLPFPLdk 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 289 PYAYASGHP--GPRILVIGDSFTQGlWEGLLAYRAS--VYGWMHHrlcrFDMAAVERFKPDILIYAPTERAMPCKGEPAH 364
Cdd:cd14440  232 PKLTTNSPAgnNKSALVFRDSFGEA-LSPYLAETFSrvVYLWSPE----IDQALIEAEKPDVVVLEIVERVLRQLPEAAP 306

                 ....*...
gi 501062371 365 wPPTPGPG 372
Cdd:cd14440  307 -PLEASPN 313
 
Name Accession Description Interval E-value
AlgX_N_like_3 cd14440
Uncharacterized proteins similar to putative alginate O-acetyltransferase; The alginate ...
57-372 2.14e-33

Uncharacterized proteins similar to putative alginate O-acetyltransferase; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. Members of this uncharacterized protein family resemble AlgX_N.


Pssm-ID: 270206 [Multi-domain]  Cd Length: 315  Bit Score: 126.32  E-value: 2.14e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  57 INNTFGFRGAVLTA--HLSYVRAmGDSMTSRVLKGEHGALFLNEDKALEQSIGQlvRPEPLGKLFDMVKLLN---DYMTA 131
Cdd:cd14440    1 FSDHFGFRDQLISAdsALLYSLL-GESSNPRVIIGKDGWLFLGEDYMLEDYCGR--DPLSEEDLRRWVALLErrrDWLAA 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 132 HGGRFVMLVPPNGHTANFELLPAYARRLKKAPTEyDLVAQRMKEAGITFVDMRPIMAEAKKTG-PVHWRYDTHWNLRGAL 210
Cdd:cd14440   78 RGIPFVVVVAPNKHTIYPEHLPSWYPGKSPTRLD-QLLALLLSAAGVGVVDLRPALLEAKATGaPVYYKTDTHWNFYGAY 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 211 LGFNAAMAAAGRPDLEVSEGDALEPPVPSYVGDLGRLSGiRPAEPDLDYpgkaPMQAPRNPVAVPGVMPEVSKTDPFV-- 288
Cdd:cd14440  157 VAYRAIAEALGPGVPALWPLASVEYDESTVGGDLANMLG-LPEALEEDR----LPDESKPPLQARRIDDDTATLPFPLdk 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 289 PYAYASGHP--GPRILVIGDSFTQGlWEGLLAYRAS--VYGWMHHrlcrFDMAAVERFKPDILIYAPTERAMPCKGEPAH 364
Cdd:cd14440  232 PKLTTNSPAgnNKSALVFRDSFGEA-LSPYLAETFSrvVYLWSPE----IDQALIEAEKPDVVVLEIVERVLRQLPEAAP 306

                 ....*...
gi 501062371 365 wPPTPGPG 372
Cdd:cd14440  307 -PLEASPN 313
ALGX pfam16822
SGNH hydrolase-like domain, acetyltransferase AlgX; ALGX is a family found in bacteria. The ...
86-319 4.97e-14

SGNH hydrolase-like domain, acetyltransferase AlgX; ALGX is a family found in bacteria. The domain demonstrates catalytic activity similar to that of the SGNH hydrolase-like domain, with the typical Ser-His-Asp triad found in this enzyme. Alginate is an exopolysaccharide that contributes to biofilm formation. ALGX is secreted into the biofilm and is responsible for the acetylation of biofilm polymers that help protect them from host destruction.


Pssm-ID: 435599  Cd Length: 267  Bit Score: 71.22  E-value: 4.97e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371   86 VLKGEHGALFLNEDkALEQSIGQLVRPEPLGklfdMVKLLNDYMTAHGGRFVMLVPPNGHTANFELLPAYaRRLKKAPTE 165
Cdd:pfam16822   1 VVLGKDGWLFTSEE-FLPNADSAAGLAARLA----LLAKVRRALAARGVKLVVVVVPDKARIYAEHLPGG-RSPSFDYSR 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  166 YDLVAQRMKEAGITFVDMRPIMAEAKKTG-PVHWRYDTHWNLRGALLGFnAAMAAAGRPDLEVSEGDA----LEPPVPS- 239
Cdd:pfam16822  75 YDQFLAALRAAGIDVPDLRPALQQAEADGkPVFLRTDTHWTPAGAEAAA-RAVAAAIRATPGFAGLPPqaftTETVGTLp 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  240 YVGDLGRLSGIRPAepdldYPGKAPMQ--APRNPVAVPGVMPEVSKTDPfvpyayasgHPGPRILVIGDSFTQGLWEGLL 317
Cdd:pfam16822 154 RPGDLANLAGLDCL-----GNRLGPRDqvPRRETTPVSASDDADGLFGD---------APAPRVALVGTSYSANPYWNFV 219

                  ..
gi 501062371  318 AY 319
Cdd:pfam16822 220 GF 221
 
Name Accession Description Interval E-value
AlgX_N_like_3 cd14440
Uncharacterized proteins similar to putative alginate O-acetyltransferase; The alginate ...
57-372 2.14e-33

Uncharacterized proteins similar to putative alginate O-acetyltransferase; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. Members of this uncharacterized protein family resemble AlgX_N.


Pssm-ID: 270206 [Multi-domain]  Cd Length: 315  Bit Score: 126.32  E-value: 2.14e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  57 INNTFGFRGAVLTA--HLSYVRAmGDSMTSRVLKGEHGALFLNEDKALEQSIGQlvRPEPLGKLFDMVKLLN---DYMTA 131
Cdd:cd14440    1 FSDHFGFRDQLISAdsALLYSLL-GESSNPRVIIGKDGWLFLGEDYMLEDYCGR--DPLSEEDLRRWVALLErrrDWLAA 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 132 HGGRFVMLVPPNGHTANFELLPAYARRLKKAPTEyDLVAQRMKEAGITFVDMRPIMAEAKKTG-PVHWRYDTHWNLRGAL 210
Cdd:cd14440   78 RGIPFVVVVAPNKHTIYPEHLPSWYPGKSPTRLD-QLLALLLSAAGVGVVDLRPALLEAKATGaPVYYKTDTHWNFYGAY 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 211 LGFNAAMAAAGRPDLEVSEGDALEPPVPSYVGDLGRLSGiRPAEPDLDYpgkaPMQAPRNPVAVPGVMPEVSKTDPFV-- 288
Cdd:cd14440  157 VAYRAIAEALGPGVPALWPLASVEYDESTVGGDLANMLG-LPEALEEDR----LPDESKPPLQARRIDDDTATLPFPLdk 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 289 PYAYASGHP--GPRILVIGDSFTQGlWEGLLAYRAS--VYGWMHHrlcrFDMAAVERFKPDILIYAPTERAMPCKGEPAH 364
Cdd:cd14440  232 PKLTTNSPAgnNKSALVFRDSFGEA-LSPYLAETFSrvVYLWSPE----IDQALIEAEKPDVVVLEIVERVLRQLPEAAP 306

                 ....*...
gi 501062371 365 wPPTPGPG 372
Cdd:cd14440  307 -PLEASPN 313
ALGX pfam16822
SGNH hydrolase-like domain, acetyltransferase AlgX; ALGX is a family found in bacteria. The ...
86-319 4.97e-14

SGNH hydrolase-like domain, acetyltransferase AlgX; ALGX is a family found in bacteria. The domain demonstrates catalytic activity similar to that of the SGNH hydrolase-like domain, with the typical Ser-His-Asp triad found in this enzyme. Alginate is an exopolysaccharide that contributes to biofilm formation. ALGX is secreted into the biofilm and is responsible for the acetylation of biofilm polymers that help protect them from host destruction.


Pssm-ID: 435599  Cd Length: 267  Bit Score: 71.22  E-value: 4.97e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371   86 VLKGEHGALFLNEDkALEQSIGQLVRPEPLGklfdMVKLLNDYMTAHGGRFVMLVPPNGHTANFELLPAYaRRLKKAPTE 165
Cdd:pfam16822   1 VVLGKDGWLFTSEE-FLPNADSAAGLAARLA----LLAKVRRALAARGVKLVVVVVPDKARIYAEHLPGG-RSPSFDYSR 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  166 YDLVAQRMKEAGITFVDMRPIMAEAKKTG-PVHWRYDTHWNLRGALLGFnAAMAAAGRPDLEVSEGDA----LEPPVPS- 239
Cdd:pfam16822  75 YDQFLAALRAAGIDVPDLRPALQQAEADGkPVFLRTDTHWTPAGAEAAA-RAVAAAIRATPGFAGLPPqaftTETVGTLp 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  240 YVGDLGRLSGIRPAepdldYPGKAPMQ--APRNPVAVPGVMPEVSKTDPfvpyayasgHPGPRILVIGDSFTQGLWEGLL 317
Cdd:pfam16822 154 RPGDLANLAGLDCL-----GNRLGPRDqvPRRETTPVSASDDADGLFGD---------APAPRVALVGTSYSANPYWNFV 219

                  ..
gi 501062371  318 AY 319
Cdd:pfam16822 220 GF 221
AlgX_N_like cd14439
N-terminal catalytic domain of putative alginate O-acetyltranferase and similar proteins; The ...
127-354 4.43e-13

N-terminal catalytic domain of putative alginate O-acetyltranferase and similar proteins; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. This wider family includes AlgX, AlgJ, AlgV, and a number of uncharacterized families, some of which may have been mis-annotated in sequence databases.


Pssm-ID: 270205 [Multi-domain]  Cd Length: 316  Bit Score: 69.40  E-value: 4.43e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 127 DYMTAHGGRFVMLVPPNGHTANFELLPAYARRLKKAPTEYDLVAQRMKEAGITFVDMRPIMAEAKKTGPVHWRYDTHWNL 206
Cdd:cd14439   74 KQLDKRGIRLLVLPVPAKAKIYPERLPAYVTPPDAVNPNYRAFLSRLRKAGVDVLDLRPVLAQAKEGEQLFYRTDHHWTP 153
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 207 RGALLGFnAAMAAAGRPDLEVSEG------DALEPPVpSYVGDLGRLSGIRPAEPdLDYPgkapmqaprnpvavpgvMPE 280
Cdd:cd14439  154 LGARLAA-QQVAEALKKKPGYEVPpekydtSKVEESR-SRLGDLAKRLGLDELLK-EDLI-----------------YLE 213
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 281 VSKTDPFVPYAYASGHPG-PRILVIGDSFTQGLWEGLLAY-----------RASVYGWM---HHRLCRFDMAAVERFK-- 343
Cdd:cd14439  214 RVVLNAGSPQSALFSDSGaPKVVLLGDSFSNVFILELLIKdgfaqhlapalGRPVDEIAkngGGSGSRRDYLAREEFKgp 293
                        250
                 ....*....|..
gi 501062371 344 -PDILIYAPTER 354
Cdd:cd14439  294 pKKVVIWEFAER 305
AlgJ_like cd14442
putative alginate O-acetyltranferases AlgJ, AlgV, and similar proteins; The alginate ...
86-244 1.57e-10

putative alginate O-acetyltranferases AlgJ, AlgV, and similar proteins; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. Members of this family have been annotated as AlgJ or AlgV, and they closely resemble AlgX in sequence and function, although they lack the C-terminal carbohydrate binding domain of AlgX.


Pssm-ID: 270208  Cd Length: 321  Bit Score: 61.55  E-value: 1.57e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371  86 VLKGEHGALFLNE----DKALEQSIGQLVRpeplgklfdMVKLLNDYMTAHGGRFVMLVPPnghtANFELLPAYARRLKK 161
Cdd:cd14442   39 VVVGKDGWLFTDEefkpAADLEANLEDNLA---------LIAEVRRALARHGVRLVLAPVP----AKARLYPEHLGGARP 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 162 AP---TEYDLVAQRMKEAGITFVDMRPIMAEAKKTGPVHWRYDTHWNLRGALLGFNAAMAAAGRPDLEVSEGDA----LE 234
Cdd:cd14442  106 PAamrSLYDRFRAALAAAGITAPDLLPALLAAKAGGPVFLRTDTHWTPAGAEVAARALAAQVRELGGLDPELQEaateAI 185
                        170
                 ....*....|
gi 501062371 235 PPVPsYVGDL 244
Cdd:cd14442  186 PPEP-HKGDL 194
AlgX_N cd14441
N-terminal catalytic domain of the putative alginate O-acetyltranferase AlgX; The alginate ...
122-251 1.09e-06

N-terminal catalytic domain of the putative alginate O-acetyltranferase AlgX; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. This N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. AlgX contains a C-terminal carbohydrate binding domain that belongs to the wider family of CBM6-CBM35-CBM36_like domains.


Pssm-ID: 270207  Cd Length: 310  Bit Score: 49.62  E-value: 1.09e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 122 VKLLNDYMTAHGGRFVMLVPPNGHTANFELLP--AYARRLK--KAPTEYDLVAQRMKEAGITFVDMRPIMAEAKKTGPVH 197
Cdd:cd14441   53 LAALSDALKARGTELVLVPQPTRGLVHAEKLPpaAYAYGFDaaVARQSYRATLARLRDAGILVPDLLPLMDTKAGGEPFF 132
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 501062371 198 WRYDTHWNLRGALLgfnAAMAAAGrpdlEVSEGDALE-PPVPSYVGDLGRLSGIR 251
Cdd:cd14441  133 FKRDHHWTPEGARA---SAKAVAE----TIRGLPAYAdLPKQAFETEPGGLLPKS 180
AlgX_N_like_1 cd14444
Uncharacterized proteins similar to putative alginate O-acetyltranferase; The alginate ...
167-308 4.39e-06

Uncharacterized proteins similar to putative alginate O-acetyltranferase; The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such those as by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. Members of this uncharacterized family similar to AlgX_N have been annotated as cell division proteins FtsQ, although they share little or no similarity with experimentally characterized members of the FtsQ family.


Pssm-ID: 270210  Cd Length: 298  Bit Score: 47.69  E-value: 4.39e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501062371 167 DLVAQRMKEAGITFVDMRPIMAEAKktGPVHWRYDTHWNLRGALLGfNAAMAAAGRPDL----EVSEGDALEPPVPSYVG 242
Cdd:cd14444  101 DAWQQALQAAGVAALDLAPALQPLG--ADAYLRTDTHWNEAGAAAA-AAAVAAAVLPLGggagPQRFFTESAGPPAPRPG 177
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 501062371 243 DLGRLSGIRPAEPDLdypgkapmQAPRNPVAVPGVMPEVSKTDPFVPYAyasghPGPRILVIGDSF 308
Cdd:cd14444  178 DLVRLAGLDWLPDGW--------RPAPESDRAVPEAAEPSRSGGLLDDA-----PLPEVALIGSSF 230
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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