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Conserved domains on  [gi|496495793|ref|WP_009204329|]
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GNAT family N-acetyltransferase [Anaerostipes hadrus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10006981)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-142 6.45e-18

Predicted N-acetyltransferase YhbS [General function prediction only];


:

Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 75.12  E-value: 6.45e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   3 IRTATIKDLEAVTSVEAECFPPAEaatRAELAERLK--YYADHFWLMFDGDKLIAFVdgfvtdeedltdeMYAKAQLHNE 80
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGR---EAELVDRLRedPAAGLSLVAEDDGEIVGHV-------------ALSPVDIDGE 64

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 496495793  81 SGAWqMIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENEG 142
Cdd:COG3153   65 GPAL-LLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPFYERFGFRPAG 125
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-142 6.45e-18

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 75.12  E-value: 6.45e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   3 IRTATIKDLEAVTSVEAECFPPAEaatRAELAERLK--YYADHFWLMFDGDKLIAFVdgfvtdeedltdeMYAKAQLHNE 80
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGR---EAELVDRLRedPAAGLSLVAEDDGEIVGHV-------------ALSPVDIDGE 64

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 496495793  81 SGAWqMIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENEG 142
Cdd:COG3153   65 GPAL-LLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPFYERFGFRPAG 125
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 11-138 7.50e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 50.98  E-value: 7.50e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   11 LEAVTSVEAECFP-PAEAATRAELAERLKYYADHFWLMFDGDKLIAFVDGFVTDEEDLTDEmyakaqlhnesgawqmIFG 89
Cdd:pfam00583   1 LEALYELLSEEFPePWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPPVGE----------------IEG 64

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 496495793   90 VNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDH---LVHYYAKFGF 138
Cdd:pfam00583  65 LAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADnlaAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-124 8.39e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 8.39e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793  44 FWLMFDGDKLIAFVDGFVTDEEDltDEMYakaqlhnesgawqmIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVL 123
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGG--DTAY--------------IGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64


                 .
gi 496495793 124 T 124
Cdd:cd04301   65 E 65
PTZ00330 PTZ00330
acetyltransferase; Provisional
 90-158 3.01e-03

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 35.97  E-value: 3.01e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 496495793  90 VNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENegvsesehggvKWNQMRL 158
Cdd:PTZ00330  88 VVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDCTEDMVAFYKKLGFRA-----------CERQMRL 145
 
Name Accession Description Interval E-value
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-142 6.45e-18

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 75.12  E-value: 6.45e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   3 IRTATIKDLEAVTSVEAECFPPAEaatRAELAERLK--YYADHFWLMFDGDKLIAFVdgfvtdeedltdeMYAKAQLHNE 80
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAFGPGR---EAELVDRLRedPAAGLSLVAEDDGEIVGHV-------------ALSPVDIDGE 64

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 496495793  81 SGAWqMIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENEG 142
Cdd:COG3153   65 GPAL-LLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPFYERFGFRPAG 125
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
70-139 3.12e-10

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 53.76  E-value: 3.12e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 496495793  70 EMYAKAQLHNESGAWQMIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDH---LVHYYAKFGFE 139
Cdd:COG3393    1 ELVAMAGVRAESPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADnpaARRLYERLGFR 73
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 1-139 4.56e-10

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 54.23  E-value: 4.56e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   1 MNIRTATIKDLEAVtsveaecfppaeaatrAELAERLKYYAD--HFWLMFDGDKLIAFVdGFVTDEEDLtdemyakAQLH 78
Cdd:COG1246    1 MTIRPATPDDVPAI----------------LELIRPYALEEEigEFWVAEEDGEIVGCA-ALHPLDEDL-------AELR 56

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 496495793  79 NesgawqmiFGVNtiPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFE 139
Cdd:COG1246   57 S--------LAVH--PDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSAAIHFYEKLGFE 107
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
41-142 7.11e-09

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 51.34  E-value: 7.11e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793  41 ADHFWLmFDGDKLIAfvdgfvtdeedltdemYAKAqLHNESGAWQmIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKG 120
Cdd:COG2153   34 ARHLLA-YDDGELVA----------------TARL-LPPGDGEAK-IGRVAVLPEYRGQGLGRALMEAAIEEARERGARR 94

                         90       100
                 ....*....|....*....|..
gi 496495793 121 LVLTCKDHLVHYYAKFGFENEG 142
Cdd:COG2153   95 IVLSAQAHAVGFYEKLGFVPVG 116
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 11-138 7.50e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 50.98  E-value: 7.50e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   11 LEAVTSVEAECFP-PAEAATRAELAERLKYYADHFWLMFDGDKLIAFVDGFVTDEEDLTDEmyakaqlhnesgawqmIFG 89
Cdd:pfam00583   1 LEALYELLSEEFPePWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPPVGE----------------IEG 64

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 496495793   90 VNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDH---LVHYYAKFGF 138
Cdd:pfam00583  65 LAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADnlaAIALYEKLGF 116
Eis COG4552
Predicted acetyltransferase [General function prediction only];
1-139 2.03e-08

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 51.82  E-value: 2.03e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   1 MNIRTATIKDLEAVTSVEAECFPPAEAATRAElAERLKYYADHFWLMFDGDKLIAFVDgfvtdeeDLTDEMYAKAQLHNE 80
Cdd:COG4552    1 MEIRPLTEDDLDAFARLLAYAFGPEPDDEELE-AYRPLLEPGRVLGVFDDGELVGTLA-------LYPFTLNVGGARVPM 72

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 496495793  81 SGawqmIFGVNTIPAYRKHGYAGELIKCAIEDARKQerkGLVLTCkdhL----VHYYAKFGFE 139
Cdd:COG4552   73 AG----ITGVAVAPEHRRRGVARALLREALAELRER---GQPLSA---LypfePGFYRRFGYE 125
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 42-139 7.01e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 47.45  E-value: 7.01e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   42 DHFWLMFDGDKLIAFVDGFVTDEEDLTdemyakaqlhnesgAWQMIFgvnTIPAYRKHGYAGELIKCAIEDARKQERKGL 121
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPLDDEGAL--------------AELRLA---VHPEYRGQGIGRALLEAAEAAAKEGGIKLL 65

                          90
                  ....*....|....*...
gi 496495793  122 VLTCKDHLVHYYAKFGFE 139
Cdd:pfam13508  66 ELETTNRAAAFYEKLGFE 83
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 72-145 3.20e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 43.11  E-value: 3.20e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 496495793  72 YAKAQLHNESGAWQmIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDH---LVHYYAKFGFENEGVSE 145
Cdd:COG0456    2 FALLGLVDGGDEAE-IEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDneaAIALYEKLGFEEVGERP 77
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 3-139 3.38e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 43.71  E-value: 3.38e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793    3 IRTATIKDLEAVTSVEAECFPPAEAATRAELaERLKYYADHFWLMFDGDKLIAFVDGFvtdeedlTDEMYakaqLHNESG 82
Cdd:pfam13527   1 IRPLTEDEFDEVLRLLEYAFQDEDSPELREY-FRPLLEEGRVLGAFDDGELVSTLALY-------PFELN----VPGKTL 68

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 496495793   83 AWQMIFGVNTIPAYRKHGYAGELIKCAIEDARKqerKGLVLTCkdhL----VHYYAKFGFE 139
Cdd:pfam13527  69 PAAGITGVATYPEYRGRGVMSRLLRRSLEEMRE---RGVPLSF---LypssYPIYRRFGYE 123
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 15-160 2.35e-05

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 41.49  E-value: 2.35e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793   15 TSVEAECFPpaEAATRAELAERLKYYADHFWLMFDGDKLIafvdGFVTdeedLTDEMYakaqlhnesgawqmIFGVNTIP 94
Cdd:pfam13673   6 SEEGIETFY--EFISPEALRERIDQGEYFFFVAFEGGQIV----GVIA----LRDRGH--------------ISLLFVDP 61

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 496495793   95 AYRKHGYAGELIKCAIEDARKQERKGLVLTCK--DHLVHYYAKFGFENEGvSESEHGGVKWNQMRLKF 160
Cdd:pfam13673  62 DYQGQGIGKALLEAVEDYAEKDGIKLSELTVNasPYAVPFYEKLGFRATG-PEQEFNGIRFVPMEKEL 128
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-124 8.39e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 8.39e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496495793  44 FWLMFDGDKLIAFVDGFVTDEEDltDEMYakaqlhnesgawqmIFGVNTIPAYRKHGYAGELIKCAIEDARKQERKGLVL 123
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGG--DTAY--------------IGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRL 64


                 .
gi 496495793 124 T 124
Cdd:cd04301   65 E 65
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 94-125 2.57e-03

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 35.19  E-value: 2.57e-03
                          10        20        30
                  ....*....|....*....|....*....|..
gi 496495793   94 PAYRKHGYAGELIKCAIEDARKQERKgLVLTC 125
Cdd:pfam14542  33 PALRGQGIASKLVKAALDDAREEGLK-IVPLC 63
PTZ00330 PTZ00330
acetyltransferase; Provisional
 90-158 3.01e-03

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 35.97  E-value: 3.01e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 496495793  90 VNTIPAYRKHGYAGELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENegvsesehggvKWNQMRL 158
Cdd:PTZ00330  88 VVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDCTEDMVAFYKKLGFRA-----------CERQMRL 145
YidJ COG2388
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
94-125 7.64e-03

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 441953 [Multi-domain]  Cd Length: 88  Bit Score: 33.98  E-value: 7.64e-03
                         10        20        30
                 ....*....|....*....|....*....|..
gi 496495793  94 PAYRKHGYAGELIKCAIEDARKQERKgLVLTC 125
Cdd:COG2388   42 PALRGQGIASALVEAALDDARERGLK-VVPLC 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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