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Conserved domains on  [gi|495115454|ref|WP_007840271|]
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DUF86 domain-containing protein [Phocaeicola dorei]

Protein Classification

DUF86 domain-containing protein( domain architecture ID 10006407)

uncharacterized DUF86 domain-containing protein

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
HEPN COG2361
HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];
11-122 1.14e-20

HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];


:

Pssm-ID: 441928  Cd Length: 115  Bit Score: 80.31  E-value: 1.14e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495115454  11 DRLTQIKESIYYIQQWSKEINcADDFLASmdkVMVFNARVMRLQVIGEQVGKLLflqPSPLSEYKEIPWLAIYDMRNLIS 90
Cdd:COG2361    8 AYLEDILEAIERIEEYTEGLD-FEDFLED---ELTQDAVIRNLEIIGEAAKRIP---DEFREKHPEIPWRQIAGMRNRLA 80
                         90       100       110
                 ....*....|....*....|....*....|..
gi 495115454  91 HEYSNVDEQIVFTTIKNDLIELDKVITVLLNK 122
Cdd:COG2361   81 HGYFGVDLEIVWDTVQNDLPELKEQIEAILEE 112
 
Name Accession Description Interval E-value
HEPN COG2361
HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];
11-122 1.14e-20

HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];


Pssm-ID: 441928  Cd Length: 115  Bit Score: 80.31  E-value: 1.14e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495115454  11 DRLTQIKESIYYIQQWSKEINcADDFLASmdkVMVFNARVMRLQVIGEQVGKLLflqPSPLSEYKEIPWLAIYDMRNLIS 90
Cdd:COG2361    8 AYLEDILEAIERIEEYTEGLD-FEDFLED---ELTQDAVIRNLEIIGEAAKRIP---DEFREKHPEIPWRQIAGMRNRLA 80
                         90       100       110
                 ....*....|....*....|....*....|..
gi 495115454  91 HEYSNVDEQIVFTTIKNDLIELDKVITVLLNK 122
Cdd:COG2361   81 HGYFGVDLEIVWDTVQNDLPELKEQIEAILEE 112
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
16-116 4.83e-09

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 50.42  E-value: 4.83e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495115454   16 IKESIYYIQQWSKEINcADDFLASMdkvMVFNARVMRLQVIGEQVGKL------LFLQPSP--------------LSEYK 75
Cdd:pfam01934   1 ILEAIERIEEFTQGLT-EEEFLSDR---LTQDAVERNLEIIGEAAIDIgnhlisDLGLRDPgsydifdilaeegvISEEP 76
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 495115454   76 EIPWLAIYDMRNLISHEYSNVDEQIVFTTIKNDLIELDKVI 116
Cdd:pfam01934  77 AIPWRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFL 117
 
Name Accession Description Interval E-value
HEPN COG2361
HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];
11-122 1.14e-20

HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];


Pssm-ID: 441928  Cd Length: 115  Bit Score: 80.31  E-value: 1.14e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495115454  11 DRLTQIKESIYYIQQWSKEINcADDFLASmdkVMVFNARVMRLQVIGEQVGKLLflqPSPLSEYKEIPWLAIYDMRNLIS 90
Cdd:COG2361    8 AYLEDILEAIERIEEYTEGLD-FEDFLED---ELTQDAVIRNLEIIGEAAKRIP---DEFREKHPEIPWRQIAGMRNRLA 80
                         90       100       110
                 ....*....|....*....|....*....|..
gi 495115454  91 HEYSNVDEQIVFTTIKNDLIELDKVITVLLNK 122
Cdd:COG2361   81 HGYFGVDLEIVWDTVQNDLPELKEQIEAILEE 112
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
16-116 4.83e-09

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 50.42  E-value: 4.83e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495115454   16 IKESIYYIQQWSKEINcADDFLASMdkvMVFNARVMRLQVIGEQVGKL------LFLQPSP--------------LSEYK 75
Cdd:pfam01934   1 ILEAIERIEEFTQGLT-EEEFLSDR---LTQDAVERNLEIIGEAAIDIgnhlisDLGLRDPgsydifdilaeegvISEEP 76
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 495115454   76 EIPWLAIYDMRNLISHEYSNVDEQIVFTTIKNDLIELDKVI 116
Cdd:pfam01934  77 AIPWRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFL 117
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
79-116 4.86e-03

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


Pssm-ID: 441985  Cd Length: 132  Bit Score: 34.49  E-value: 4.86e-03
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 495115454  79 WLAIYDMRNLISHEYSNVDEQIVFTTIKNDLIELDKVI 116
Cdd:COG2445   90 LKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFI 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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