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Conserved domains on  [gi|493924035|ref|WP_006868936|]
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nitroreductase/quinone reductase family protein [Gordonia namibiensis]

Protein Classification

PNPOx family protein( domain architecture ID 1004)

PNPOx family protein similar to Escherichia coli pyridoxamine 5'-phosphate oxidase (PNPOx), a FMN flavoprotein that catalyzes the oxidation of pyridoxamine-5-P (PMP) and pyridoxine-5-P (PNP) to pyridoxal-5-P (PLP)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PNPOx/FlaRed_like super family cl00381
Pyridoxine 5'-phosphate (PNP) oxidase-like and flavin reductase-like proteins; The PNPOx-like ...
 12-89 2.38e-20

Pyridoxine 5'-phosphate (PNP) oxidase-like and flavin reductase-like proteins; The PNPOx-like superfamily is composed of pyridoxine 5'-phosphate (PNP) oxidases and other flavin mononucleotide (FMN) binding proteins, which catalyze FMN-mediated redox reactions. Flavin reductases catalyze the reduction of FMN or FAD using NAD(P)H as a cofactor. They are part of a two-component enzyme system, composed of a smaller component (flavin reductase) and a larger component (an oxidase), which uses the reduced flavin to hydroxylate substrates.


The actual alignment was detected with superfamily member pfam04075:

Pssm-ID: 469750  Cd Length: 129  Bit Score: 80.57  E-value: 2.38e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493924035   12 FRANNGHVETAGFGKNLVLLHTLGAKSGEPRISPLFGLADGGSWLVIGSFAGSPTTPAWVHNLRAHP-----------DA 80
Cdd:pfam04075   9 YRRTGGTVGRRLPGVPVLLLTTTGRKTGQPRTTPLGGVRDGDSYLVVASKGGAPRHPGWYYNLKANPrvrvqiggrwlDG 88


                  ....*....
gi 493924035   81 VAVEAPAPE 89
Cdd:pfam04075  89 TARLAPDDE 97
 
Name Accession Description Interval E-value
F420H2_quin_red pfam04075
F420H(2)-dependent quinone reductase; This family of proteins is found in the genera ...
 12-89 2.38e-20

F420H(2)-dependent quinone reductase; This family of proteins is found in the genera Mycobacterium and Streptomyces. Member protein Rv3547 has been characterized as a deazaflavin-dependent nitroreductase. Rv1558 is an F420H(2)-dependent quinone reductase involved in oxidative stress protection.


Pssm-ID: 281995  Cd Length: 129  Bit Score: 80.57  E-value: 2.38e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493924035   12 FRANNGHVETAGFGKNLVLLHTLGAKSGEPRISPLFGLADGGSWLVIGSFAGSPTTPAWVHNLRAHP-----------DA 80
Cdd:pfam04075   9 YRRTGGTVGRRLPGVPVLLLTTTGRKTGQPRTTPLGGVRDGDSYLVVASKGGAPRHPGWYYNLKANPrvrvqiggrwlDG 88


                  ....*....
gi 493924035   81 VAVEAPAPE 89
Cdd:pfam04075  89 TARLAPDDE 97
hi_GC_TIGR00026 TIGR00026
deazaflavin-dependent oxidoreductase, nitroreductase family; This model represents a family of ...
 21-138 9.06e-19

deazaflavin-dependent oxidoreductase, nitroreductase family; This model represents a family of proteins found in paralogous families in the genera Mycobacterium and Streptomyces. Seven members are in Mycobacterium tuberculosis. Member protein Rv3547 has been characterized as a deazaflavin-dependent nitroreductase. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 211538  Cd Length: 113  Bit Score: 75.91  E-value: 9.06e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493924035   21 TAGFGKNLVLLHTLGAKSGEPRISPLFGLADGGSWLVIGSFAGSPTTPAWVHNLRAHPDaVAVEApapeGIERHETQVIE 100
Cdd:TIGR00026   2 GIARGLPVLLLTTTGRKTGKPRTTPVYYVRHDPGVLVVASNGGAPRHPDWYKNLKANPR-VRVRV----GGKVFVATARL 76

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 493924035  101 MTDADWDAAWAKFTAFTDQFIKYTETAqGRKFPIFRLT 138
Cdd:TIGR00026  77 VSGDERAQLWAGVVRLYPRYQRYQSRT-DRPIPVVVLT 113
 
Name Accession Description Interval E-value
F420H2_quin_red pfam04075
F420H(2)-dependent quinone reductase; This family of proteins is found in the genera ...
 12-89 2.38e-20

F420H(2)-dependent quinone reductase; This family of proteins is found in the genera Mycobacterium and Streptomyces. Member protein Rv3547 has been characterized as a deazaflavin-dependent nitroreductase. Rv1558 is an F420H(2)-dependent quinone reductase involved in oxidative stress protection.


Pssm-ID: 281995  Cd Length: 129  Bit Score: 80.57  E-value: 2.38e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493924035   12 FRANNGHVETAGFGKNLVLLHTLGAKSGEPRISPLFGLADGGSWLVIGSFAGSPTTPAWVHNLRAHP-----------DA 80
Cdd:pfam04075   9 YRRTGGTVGRRLPGVPVLLLTTTGRKTGQPRTTPLGGVRDGDSYLVVASKGGAPRHPGWYYNLKANPrvrvqiggrwlDG 88


                  ....*....
gi 493924035   81 VAVEAPAPE 89
Cdd:pfam04075  89 TARLAPDDE 97
hi_GC_TIGR00026 TIGR00026
deazaflavin-dependent oxidoreductase, nitroreductase family; This model represents a family of ...
 21-138 9.06e-19

deazaflavin-dependent oxidoreductase, nitroreductase family; This model represents a family of proteins found in paralogous families in the genera Mycobacterium and Streptomyces. Seven members are in Mycobacterium tuberculosis. Member protein Rv3547 has been characterized as a deazaflavin-dependent nitroreductase. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 211538  Cd Length: 113  Bit Score: 75.91  E-value: 9.06e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493924035   21 TAGFGKNLVLLHTLGAKSGEPRISPLFGLADGGSWLVIGSFAGSPTTPAWVHNLRAHPDaVAVEApapeGIERHETQVIE 100
Cdd:TIGR00026   2 GIARGLPVLLLTTTGRKTGKPRTTPVYYVRHDPGVLVVASNGGAPRHPDWYKNLKANPR-VRVRV----GGKVFVATARL 76

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 493924035  101 MTDADWDAAWAKFTAFTDQFIKYTETAqGRKFPIFRLT 138
Cdd:TIGR00026  77 VSGDERAQLWAGVVRLYPRYQRYQSRT-DRPIPVVVLT 113
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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