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Conserved domains on  [gi|492414672|ref|WP_005837253|]
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MULTISPECIES: methylated-DNA--[protein]-cysteine S-methyltransferase [Bacteroides]

Protein Classification

methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11417447)

methylated-DNA--[protein]-cysteine S-methyltransferase is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA; it repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme

CATH:  3.30.160.70|1.10.10.10
EC:  2.1.1.63
Gene Ontology:  GO:0003908|GO:0003677|GO:0046872|GO:0006281|GO:0032259
PubMed:  17500045|17485252
SCOP:  4000565|4000678

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 3-167 4.53e-51

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 161.58  E-value: 4.53e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   3 TILTQYYQSPYGELLLGSFGDKLCLCDWMlnEKRRVPVDKRIRQALHADyeegtSEVIARAIIQLDEYFACKRTIFDVPL 82
Cdd:COG0350    1 TIRYAIFDTPLGPLLIAATDRGLCALSFG--DDREEALLARFPAALRED-----PPLLAEAARQLDAYFAGERKDFDLPL 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  83 LAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKavravaaanganaLSILIPCHRVVGSNHTLIGYAGGLAAKQGM 162
Cdd:COG0350   74 DLRGTPFQRRVWEALRKIPYGETVTYGELARAIGRPKaaravgsacganpIPIIIPCHRVIGADGSLGGYAGGLERKRAL 153


                 ....*
gi 492414672 163 LALEA 167
Cdd:COG0350  154 LELEG 158
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 3-167 4.53e-51

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 161.58  E-value: 4.53e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   3 TILTQYYQSPYGELLLGSFGDKLCLCDWMlnEKRRVPVDKRIRQALHADyeegtSEVIARAIIQLDEYFACKRTIFDVPL 82
Cdd:COG0350    1 TIRYAIFDTPLGPLLIAATDRGLCALSFG--DDREEALLARFPAALRED-----PPLLAEAARQLDAYFAGERKDFDLPL 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  83 LAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKavravaaanganaLSILIPCHRVVGSNHTLIGYAGGLAAKQGM 162
Cdd:COG0350   74 DLRGTPFQRRVWEALRKIPYGETVTYGELARAIGRPKaaravgsacganpIPIIIPCHRVIGADGSLGGYAGGLERKRAL 153


                 ....*
gi 492414672 163 LALEA 167
Cdd:COG0350  154 LELEG 158
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 8-166 1.70e-40

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 134.41  E-value: 1.70e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   8 YYQSPYGELLLGS---------FGDKLCLCDWMLNEKrrvpvdkrirqalhadyeegtsEVIARAIIQLDEYFACKRTIF 78
Cdd:PRK00901   6 FYETPIGKIGIAEngtaithlcFGEDKIPKDVTILET----------------------DLLKEANKQLEEYFEGKRKKF 63

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  79 DVPLLAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAA 158
Cdd:PRK00901  64 DLPLAPQGTEFQKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDI 143


                 ....*...
gi 492414672 159 KQGMLALE 166
Cdd:PRK00901 144 KEKLLKLE 151
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 87-166 4.06e-29

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 102.78  E-value: 4.06e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   87 TEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALE 166
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 88-167 1.33e-26

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 96.28  E-value: 1.33e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   88 EFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALEA 167
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 89-166 6.73e-25

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 91.77  E-value: 6.73e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 492414672  89 FQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALE 166
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELE 78
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 3-167 4.53e-51

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 161.58  E-value: 4.53e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   3 TILTQYYQSPYGELLLGSFGDKLCLCDWMlnEKRRVPVDKRIRQALHADyeegtSEVIARAIIQLDEYFACKRTIFDVPL 82
Cdd:COG0350    1 TIRYAIFDTPLGPLLIAATDRGLCALSFG--DDREEALLARFPAALRED-----PPLLAEAARQLDAYFAGERKDFDLPL 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  83 LAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKavravaaanganaLSILIPCHRVVGSNHTLIGYAGGLAAKQGM 162
Cdd:COG0350   74 DLRGTPFQRRVWEALRKIPYGETVTYGELARAIGRPKaaravgsacganpIPIIIPCHRVIGADGSLGGYAGGLERKRAL 153


                 ....*
gi 492414672 163 LALEA 167
Cdd:COG0350  154 LELEG 158
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 8-166 1.70e-40

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 134.41  E-value: 1.70e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   8 YYQSPYGELLLGS---------FGDKLCLCDWMLNEKrrvpvdkrirqalhadyeegtsEVIARAIIQLDEYFACKRTIF 78
Cdd:PRK00901   6 FYETPIGKIGIAEngtaithlcFGEDKIPKDVTILET----------------------DLLKEANKQLEEYFEGKRKKF 63

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  79 DVPLLAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAA 158
Cdd:PRK00901  64 DLPLAPQGTEFQKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDI 143


                 ....*...
gi 492414672 159 KQGMLALE 166
Cdd:PRK00901 144 KEKLLKLE 151
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 87-166 4.06e-29

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 102.78  E-value: 4.06e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   87 TEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALE 166
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 88-167 1.33e-26

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 96.28  E-value: 1.33e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   88 EFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALEA 167
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 89-166 6.73e-25

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 91.77  E-value: 6.73e-25
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 492414672  89 FQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALE 166
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELE 78
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
66-166 1.60e-23

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 91.08  E-value: 1.60e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  66 QLDEYFACKRTIFD-VPLLAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVG 144
Cdd:PRK10286  65 KLRDYFAGNLSIIDtLPTATGGTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIG 144

                         90       100
                 ....*....|....*....|..
gi 492414672 145 SNHTLIGYAGGLAAKQGMLALE 166
Cdd:PRK10286 145 RNGTMTGYAGGVQRKEWLLRHE 166
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 2-167 1.79e-20

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 86.65  E-value: 1.79e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672   2 ETILTQYYQSPYGELLLgsFGDKLCLCDWMLNEKRRVPVDKRIRQALHADYEEGTSEvIARAIIQLDEYFACKRTIFDVP 81
Cdd:COG2169  188 AAIRFAPTPCSLGLLLV--AASARGVCAILLGDDPEALLRDLQDRFPAAELIGGDAA-FEQLVAEVVGFVEGPLLGLDLP 264

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  82 LLAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVAAANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQG 161
Cdd:COG2169  265 LDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAIPCHRVVRADGALSGYRWGVERKRA 344


                 ....*.
gi 492414672 162 MLALEA 167
Cdd:COG2169  345 LLEREA 350
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
49-167 1.19e-19

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 84.46  E-value: 1.19e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  49 HADYEEGTSEV---IARAIIQLDEyfacKRTIFDVPLLAVGTEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKAVRAVA 125
Cdd:PRK15435 232 AADNAPADLTFqqhVREVIASLNQ----RDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVA 307

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 492414672 126 AANGANALSILIPCHRVVGSNHTLIGYAGGLAAKQGMLALEA 167
Cdd:PRK15435 308 SACAANKLAIVIPCHRVVRGDGALSGYRWGVSRKAQLLRREA 349
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 87-168 4.20e-05

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 40.94  E-value: 4.20e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  87 TEFQRTVWQELQNIPYGKTVSYGELSRKLGNPKavravaaanganalS------IL--------IPCHRVVGSNHTLIGY 152
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPR--------------GarqvgrALralpegsdLPWHRVVNADGRLSPG 69

                         90
                 ....*....|....*.
gi 492414672 153 AGGLAAKQGMLaLEAD 168
Cdd:COG3695   70 HAGGAEEQREL-LEAE 84
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 87-166 1.41e-03

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 37.79  E-value: 1.41e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 492414672  87 TEFQRTVWQEL-QNIPYGKTVSYGELSRKLG------------NPkavravaaanganaLSILIPCHRVVGSNHtlIG-Y 152
Cdd:PRK03887  91 TPFERKVYEWLtKNVKRGEVITYGELAKALNtspravggamkrNP--------------YPIIVPCHRVVGRKN--PGlY 154

                         90
                 ....*....|....
gi 492414672 153 AGGLAAKQGMLALE 166
Cdd:PRK03887 155 TPKPEYKKFLLEVE 168
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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