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Conserved domains on  [gi|490950659|ref|WP_004812479|]
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competence type IV pilus minor pilin ComGE [Anaerococcus hydrogenalis]

Protein Classification

type II secretion system protein( domain architecture ID 11450365)

type II secretion system protein similar to Pseudomonas aeruginosa type II secretion system protein H, which is involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins; required for the translocation of a variety of enzymes across the outer membrane; pilin similar to fimbrial protein PilE, which is required for pilus biogenesis, as well as fimbrial protein PilA, which is responsible for the adhesive property of the pilus

Gene Ontology:  GO:0005886|GO:0015628|GO:0015627
PubMed:  30767847|1365398

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
2-84 3.03e-14

competence type IV pilus minor pilin ComGE;


:

Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 62.22  E-value: 3.03e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   2 KKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKSLFIMQEVLERSKeREIGQYEDEIDGIKTYTQVISY 81
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSLLLPSLTQIQKEREEIKQKEEALQVLYEALQTYQ-LHLELNGVEITVERSNTQIKVY 79


                 ...
gi 490950659  82 NEN 84
Cdd:NF041013  80 WEG 82
 
Name Accession Description Interval E-value
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
2-84 3.03e-14

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 62.22  E-value: 3.03e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   2 KKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKSLFIMQEVLERSKeREIGQYEDEIDGIKTYTQVISY 81
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSLLLPSLTQIQKEREEIKQKEEALQVLYEALQTYQ-LHLELNGVEITVERSNTQIKVY 79


                 ...
gi 490950659  82 NEN 84
Cdd:NF041013  80 WEG 82
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-84 1.04e-10

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 53.76  E-value: 1.04e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSkSLFIMQEVLERSKeREIGQYEDEIDGIKTYTQVIS 80
Cdd:COG2165    7 RRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRS-NLRQIQQALERYR-LDNGRYPSSLTGLLADVRGGG 84


                 ....
gi 490950659  81 YNEN 84
Cdd:COG2165   85 YLGS 88
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 1.15e-06

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 41.20  E-value: 1.15e-06
                          10        20
                  ....*....|....*....|....*..
gi 490950659    1 MKKKKGFTLIEVIISLALIGIISLYIL 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 4-27 3.16e-05

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 37.67  E-value: 3.16e-05
                          10        20
                  ....*....|....*....|....
gi 490950659    4 KKGFTLIEVIISLALIGIISLYIL 27
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
PRK10574 PRK10574
putative major pilin subunit; Provisional
 1-29 4.79e-04

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 36.93  E-value: 4.79e-04
                         10        20
                 ....*....|....*....|....*....
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPS 29
Cdd:PRK10574   1 MDKQRGFTLIELMVVIAIIAILSAIGIPA 29
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 5-94 1.19e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 35.67  E-value: 1.19e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   5 KGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKS---LFIMQ--EVLERSKEREIGQYED-EIDGIKTYTQV 78
Cdd:NF041002   2 KGFTLLECLVALLVLSLILLLIPLLIKLLLKVEQYLNNSDQLewlLFLQQleSELKESTFKVKNNKLYvKLDLIKSNGKT 81

                         90
                 ....*....|....*..
gi 490950659  79 ISYNE-NLKYIKVKNSK 94
Cdd:NF041002  82 VAYEKyNSDIRKRVNGK 98
 
Name Accession Description Interval E-value
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
2-84 3.03e-14

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 62.22  E-value: 3.03e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   2 KKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKSLFIMQEVLERSKeREIGQYEDEIDGIKTYTQVISY 81
Cdd:NF041013   1 KKNKGFILLESLVALALLALIVSLLLPSLTQIQKEREEIKQKEEALQVLYEALQTYQ-LHLELNGVEITVERSNTQIKVY 79


                 ...
gi 490950659  82 NEN 84
Cdd:NF041013  80 WEG 82
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-84 1.04e-10

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 53.76  E-value: 1.04e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSkSLFIMQEVLERSKeREIGQYEDEIDGIKTYTQVIS 80
Cdd:COG2165    7 RRRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRS-NLRQIQQALERYR-LDNGRYPSSLTGLLADVRGGG 84


                 ....
gi 490950659  81 YNEN 84
Cdd:COG2165   85 YLGS 88
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-38 7.95e-08

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 45.22  E-value: 7.95e-08
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSK 38
Cdd:COG4970    5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQR 42
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 1.15e-06

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 41.20  E-value: 1.15e-06
                          10        20
                  ....*....|....*....|....*..
gi 490950659    1 MKKKKGFTLIEVIISLALIGIISLYIL 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
1-52 2.08e-06

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 43.63  E-value: 2.08e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLyilpSIFSVYKNSKKIKDDSKSLFIMQE 52
Cdd:COG4966    1 RRRQRGFTLVELMVALAIGLIVLA----AVLQLFLSSRRSYRTQEALARLQE 48
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
1-29 4.29e-06

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 41.98  E-value: 4.29e-06
                         10        20
                 ....*....|....*....|....*....
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPS 29
Cdd:COG4968    6 RRRQRGFTLIELMIVVAIIGILAAIAIPS 34
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-82 1.19e-05

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 39.97  E-value: 1.19e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKSLFIMQEVLE--RSKEREIGQYedeiDGIKTYTQV 78
Cdd:COG4967    7 RRRQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLErlRANPAAAGSY----PGIKSITVT 82


                 ....
gi 490950659  79 ISYN 82
Cdd:COG4967   83 VRWQ 86
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 4-27 3.16e-05

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 37.67  E-value: 3.16e-05
                          10        20
                  ....*....|....*....|....
gi 490950659    4 KKGFTLIEVIISLALIGIISLYIL 27
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
1-29 4.28e-05

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 39.69  E-value: 4.28e-05
                         10        20        30
                 ....*....|....*....|....*....|
gi 490950659   1 MKKK-KGFTLIEVIISLALIGIISLYILPS 29
Cdd:COG4969    1 MKKKqKGFTLIELMIVVAIIGILAAIAIPA 30
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-24 1.03e-04

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 38.46  E-value: 1.03e-04
                         10        20
                 ....*....|....*....|....
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISL 24
Cdd:COG4795    5 RRRQRGFTLLELLVALAIFALLLL 28
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
1-42 1.08e-04

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 37.98  E-value: 1.08e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPsifSVYKNSKKIKD 42
Cdd:COG4537    8 LKKEKGFTLIEMLIVLLIISILLLIAVP---NLTKQRETAQE 46
PRK10574 PRK10574
putative major pilin subunit; Provisional
 1-29 4.79e-04

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 36.93  E-value: 4.79e-04
                         10        20
                 ....*....|....*....|....*....
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISLYILPS 29
Cdd:PRK10574   1 MDKQRGFTLIELMVVIAIIAILSAIGIPA 29
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 5-94 1.19e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 35.67  E-value: 1.19e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490950659   5 KGFTLIEVIISLALIGIISLYILPSIFSVYKNSKKIKDDSKS---LFIMQ--EVLERSKEREIGQYED-EIDGIKTYTQV 78
Cdd:NF041002   2 KGFTLLECLVALLVLSLILLLIPLLIKLLLKVEQYLNNSDQLewlLFLQQleSELKESTFKVKNNKLYvKLDLIKSNGKT 81

                         90
                 ....*....|....*..
gi 490950659  79 ISYNE-NLKYIKVKNSK 94
Cdd:NF041002  82 VAYEKyNSDIRKRVNGK 98
PRK10506 PRK10506
prepilin peptidase-dependent protein;
1-24 1.37e-03

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 35.74  E-value: 1.37e-03
                         10        20
                 ....*....|....*....|....
gi 490950659   1 MKKKKGFTLIEVIISLALIGIISL 24
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSA 28
typeII_sec_gspG TIGR01710
type II secretion system protein G; This model represents GspG, protein G of the main terminal ...
 5-32 2.40e-03

type II secretion system protein G; This model represents GspG, protein G of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130771 [Multi-domain]  Cd Length: 134  Bit Score: 34.71  E-value: 2.40e-03
                          10        20
                  ....*....|....*....|....*...
gi 490950659    5 KGFTLIEVIISLALIGIISLYILPSIFS 32
Cdd:TIGR01710   1 RGFTLLEIMVVLVILGLLAALVAPKLFS 28
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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