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Conserved domains on  [gi|490745974|ref|WP_004608282|]
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NAD(P)H-dependent oxidoreductase subunit E [[Clostridium] scindens]

Protein Classification

complex I 24 kDa subunit family protein( domain architecture ID 11112961)

complex I 24 kDa subunit family protein is a thioredoxin-like ferredoxin subunit of an NADH dehydrogenase is involved in the reversible oxidation of molecular hydrogen using NADH or NADPH as electron donor or acceptor; such as bacterial NADH-quinone oxidoreductase subunit E (NuoE) and eukaryotic NADH-ubiquinone oxidoreductase 24 kDa subunit

EC:  7.1.1.-
Gene Ontology:  GO:0051537|GO:0016491
PubMed:  15916556|30277212

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 13-164 2.84e-77

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


:

Pssm-ID: 441509  Cd Length: 159  Bit Score: 226.95  E-value: 2.84e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  13 TKEQEKELLEVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLG 92
Cdd:COG1905    7 SEELLAEIDEIIAKYPRKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKHVIRVCTG 86

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490745974  93 TACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:COG1905   87 TACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELR 158
 
Name Accession Description Interval E-value
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 13-164 2.84e-77

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 226.95  E-value: 2.84e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  13 TKEQEKELLEVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLG 92
Cdd:COG1905    7 SEELLAEIDEIIAKYPRKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKHVIRVCTG 86

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490745974  93 TACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:COG1905   87 TACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELR 158
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
22-164 6.47e-72

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 212.72  E-value: 6.47e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974   22 EVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSG 101
Cdd:pfam01257   3 EIIAKYPRKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHLRGSD 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 490745974  102 DIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:pfam01257  83 EILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
13-164 6.31e-55

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 169.95  E-value: 6.31e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  13 TKEQEKELLEVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLG 92
Cdd:PRK07539   3 SAEELAAIEREIAKYPRPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQVCTS 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490745974  93 TACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:PRK07539  83 TPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 30-164 2.60e-46

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 148.29  E-value: 2.60e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974   30 EKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSGDIYNKLME 109
Cdd:TIGR01958  14 KRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCALRGSEALLKYLEN 93

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 490745974  110 KLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:TIGR01958  94 KLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 84-163 1.76e-36

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 120.70  E-value: 1.76e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  84 RYRISVCLGTACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKY 163
Cdd:cd03064    1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
 
Name Accession Description Interval E-value
NuoE COG1905
NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; ...
 13-164 2.84e-77

NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) [Energy production and conversion]; NADH:ubiquinone oxidoreductase 24 kD subunit (chain E) is part of the Pathway/BioSystem: NADH dehydrogenase


Pssm-ID: 441509  Cd Length: 159  Bit Score: 226.95  E-value: 2.84e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  13 TKEQEKELLEVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLG 92
Cdd:COG1905    7 SEELLAEIDEIIAKYPRKRSALIPILHLVQEEFGYLPEEAIEEIAEALGLPPAEVYGVATFYSMFRLKPVGKHVIRVCTG 86

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490745974  93 TACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:COG1905   87 TACHLRGAEELLEALEEKLGIKPGETTADGKFTLEEVECLGACGLAPVMMVNDDVYGRLTPEKVDEILDELR 158
2Fe-2S_thioredx pfam01257
Thioredoxin-like [2Fe-2S] ferredoxin;
22-164 6.47e-72

Thioredoxin-like [2Fe-2S] ferredoxin;


Pssm-ID: 460139  Cd Length: 145  Bit Score: 212.72  E-value: 6.47e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974   22 EVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSG 101
Cdd:pfam01257   3 EIIAKYPRKRSALIPILHLAQEEYGYLPPEAIEYVAELLGIPPARVYEVATFYTMFSLKPVGKYHIQVCTGTPCHLRGSD 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 490745974  102 DIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:pfam01257  83 EILEALEKKLGIKPGETTPDGKFTLEEVECLGACDNAPVMQINDDYYGDLTPEKVDEILEELK 145
PRK07539 PRK07539
NADH-quinone oxidoreductase subunit NuoE;
13-164 6.31e-55

NADH-quinone oxidoreductase subunit NuoE;


Pssm-ID: 181024  Cd Length: 154  Bit Score: 169.95  E-value: 6.31e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  13 TKEQEKELLEVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLG 92
Cdd:PRK07539   3 SAEELAAIEREIAKYPRPRSAVIPALKIVQEQRGWVPDEAIEAVADYLGMPAIDVEEVATFYSMIFRQPVGRHVIQVCTS 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490745974  93 TACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:PRK07539  83 TPCWLRGGEAILAALKKKLGIKPGETTADGRFTLLEVECLGACDNAPVVMINDDTYEDLTPEKIDELLDELK 154
nuoE_fam TIGR01958
NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that ...
 30-164 2.60e-46

NADH-quinone oxidoreductase, E subunit; This model describes the E chain of complexes that resemble NADH-quinone oxidoreductases. The electron acceptor is a quinone, ubiquinone, in mitochondria and most bacteria, including Escherichia coli, where the recommended gene symbol is nuoB. This model does not identify proteins from chloroplast and cyanobacteria. [Energy metabolism, Electron transport]


Pssm-ID: 131013  Cd Length: 148  Bit Score: 148.29  E-value: 2.60e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974   30 EKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSGDIYNKLME 109
Cdd:TIGR01958  14 KRSAIMPALMIAQEQKGWVTPEAIAAVAEMLGIPPVWVYEVATFYSMFDTEPVGRYHLQVCTNVPCALRGSEALLKYLEN 93

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 490745974  110 KLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:TIGR01958  94 KLGIKPGETTPDGRFTLVEVECLGACGNAPVMMINDDYYEFLTPEKLDELLERYE 148
PRK07571 PRK07571
bidirectional hydrogenase complex protein HoxE; Reviewed
 33-156 9.96e-46

bidirectional hydrogenase complex protein HoxE; Reviewed


Pssm-ID: 236053  Cd Length: 169  Bit Score: 147.21  E-value: 9.96e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  33 SLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSGDIYNKLMEKLG 112
Cdd:PRK07571  37 ALIEVLHKAQELFGYLERDLLLYVARQLKLPLSRVYGVATFYHLFSLKPSGEHTCVVCTGTACYVKGSAAILEDLENELG 116

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 490745974 113 IVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDI 156
Cdd:PRK07571 117 IKAGETTADGKLSLLTARCLGACGIAPAVVFDGKVAGKQTPESV 160
TRX_Fd_NuoE cd03064
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 84-163 1.76e-36

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of eukaryotes. It is a multisubunit complex with at least 14 core subunits. It catalyzes the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane, providing the proton motive force required for energy-consuming processes. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE core subunit, also called the 24 kD subunit of Complex 1. This subfamily also include formate dehydrogenases, NiFe hydrogenases and NAD-reducing hydrogenases, that contain a NuoE domain. A subset of these proteins contain both NuoE and NuoF in a single chain. NuoF, also called the 51 kD subunit of Complex 1, contains one [4Fe-4S] cluster and also binds the NADH substrate and FMN.


Pssm-ID: 239362 [Multi-domain]  Cd Length: 80  Bit Score: 120.70  E-value: 1.76e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  84 RYRISVCLGTACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKY 163
Cdd:cd03064    1 KHVIRVCTGTACHLRGAEALLEALEKKLGIKPGETTPDGRFTLEEVECLGACDLAPVMMINDDVYGRLTPEKVDAILEAL 80
PRK05988 PRK05988
formate dehydrogenase subunit gamma; Validated
 22-161 5.44e-34

formate dehydrogenase subunit gamma; Validated


Pssm-ID: 180339  Cd Length: 156  Bit Score: 116.98  E-value: 5.44e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  22 EVIHELKDEKGSLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKGRYRISVCLGTACYVKGSG 101
Cdd:PRK05988  13 AIIAEHKHLEGALLPILHAIQDEFGYVPEDAVPVIAEALNLSRAEVHGVITFYHDFRTHPPGRHVLKLCRAEACQAMGGD 92

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974 102 DIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILA 161
Cdd:PRK05988  93 ALAAHAKARLGIDFHQTTADGAVTLEPVYCLGLCACSPAAMLDGEVHGRLDPQRLDALLA 152
TRX_Fd_family cd02980
Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX ...
 85-163 4.06e-24

Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX fold (TRX-like Fds) and proteins containing domains similar to TRX-like Fd including formate dehydrogenases, NAD-reducing hydrogenases and the subunit E of NADH:ubiquinone oxidoreductase (NuoE). TRX-like Fds are soluble low-potential electron carriers containing a single [2Fe-2S] cluster. The exact role of TRX-like Fd is still unclear. It has been suggested that it may be involved in nitrogen fixation. Its homologous domains in large redox enzymes (such as Nuo and hydrogenases) function as electron carriers.


Pssm-ID: 239278 [Multi-domain]  Cd Length: 77  Bit Score: 89.22  E-value: 4.06e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  85 YRISVCLGTACYVKGSGDIYNKLMEKLGIVGGectpDGKFSLDACRCVGACGLAPVMMVN--DEVYGRLTVDDIDDILAK 162
Cdd:cd02980    1 HHILVCTGTACGLRGAEELLEALEKELGIRGG----DGRVTVERVGCLGACGLAPVVVVYpdGVWYGRVTPEDVEEIVEE 76


                 .
gi 490745974 163 Y 163
Cdd:cd02980   77 L 77
PRK12373 PRK12373
NADH-quinone oxidoreductase subunit E;
33-164 1.55e-15

NADH-quinone oxidoreductase subunit E;


Pssm-ID: 237082 [Multi-domain]  Cd Length: 400  Bit Score: 72.53  E-value: 1.55e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  33 SLMPILQRAQDIYGYLPIEVQKIISNETGIPLEKIYGVVTFYSQFNLNPKG-RYRISVCLGTACYVKGSGDIYNKLMEKL 111
Cdd:PRK12373  38 AVIPLLMRAQEQEGWVTRAAIEKVADMLDMAYIRVLEVATFYTQFQLQPVGtRAHIQVCGTTPCMLRGSEALMAVCKSKI 117

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 490745974 112 GIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILAKYE 164
Cdd:PRK12373 118 HAHPHELNADGTLSWEEVECLGACVNAPMVQIGKDYYEDLTPERLEEIIDAFA 170
TRX_Fd_NuoE_FDH_gamma cd03081
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 84-161 4.45e-15

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, NAD-dependent formate dehydrogenase (FDH) gamma subunit; composed of proteins similar to the gamma subunit of NAD-linked FDH of Ralstonia eutropha, a soluble enzyme that catalyzes the irreversible oxidation of formate to carbon dioxide accompanied by the reduction of NAD+ to NADH. FDH is a heteromeric enzyme composed of four nonidentical subunits (alpha, beta, gamma and delta). The FDH gamma subunit is closely related to NuoE, which is part of a multisubunit complex (Nuo) catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster present in NuoE. Similarly, the FDH gamma subunit is hypothesized to be involved in an electron transport chain involving other FDH subunits, upon the oxidation of formate.


Pssm-ID: 239379  Cd Length: 80  Bit Score: 66.25  E-value: 4.45e-15
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 490745974  84 RYRISVCLGTACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILA 161
Cdd:cd03081    1 RHVLKLCRAEACQAMGAEALAAHIKARLGIDFHETTADGSVTLEPVYCLGLCACSPAAMIDGEVHGRVDPEKFDALLA 78
TRX_Fd_NuoE_hoxF cd03083
TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E ...
 84-161 8.70e-13

TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily, hoxF; composed of proteins similar to the NAD-reducing hydrogenase (hoxS) alpha subunit of Alcaligenes eutrophus H16. HoxS is a cytoplasmic hydrogenase catalyzing the oxidation of molecular hydrogen accompanied by the reduction of NAD. It is composed of four structural subunits encoded by the genes hoxF, hoxU, hoxY and hoxH. The hoxF protein (or alpha subunit) is a fusion protein containing an N-terminal NuoE-like domain and a C-terminal NuoF domain. NuoE and NuoF are components of Nuo, a multisubunit complex catalyzing the electron transfer of NADH to quinone coupled with the transfer of protons across the membrane. Electrons are transferred from NADH to quinone through a chain of iron-sulfur clusters in Nuo, including the [2Fe-2S] cluster in NuoE and the [4Fe-4S] cluster in NuoF. In addition, NuoF is also the NADH- and FMN-binding subunit. HoxF may be involved in the electron transport chain during the NAD-dependent oxidation of hydrogen through its NuoF domain. The NuoE-like domain of hoxF contains only one conserved cysteine in its putative active site, compared to four cysteines in NuoE, and may have lost the ability to bind [2Fe-2S] clusters.


Pssm-ID: 239381  Cd Length: 80  Bit Score: 60.12  E-value: 8.70e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 490745974  84 RYRISVCLGTACYVKGSGDIYNKLMEKLGIVGGECTPDGKFSLDACRCVGACGLAPVMMVNDEVYGRLTVDDIDDILA 161
Cdd:cd03083    1 KYRIYLSDSITDRMNGYKAVLDALCRELGIRFGEVDEDGMVGLFFTSCTGLCDQGPALLINNRVFTRLTPGRIDQIAE 78
COG3411 COG3411
2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway ...
 81-163 8.42e-08

2Fe-2S ferredoxin [Energy production and conversion]; 2Fe-2S ferredoxin is part of the Pathway/BioSystem: 2Fe2S


Pssm-ID: 442637  Cd Length: 96  Bit Score: 47.58  E-value: 8.42e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490745974  81 PKGRYRISVCLGTaCYVKGSGDIYNKLMEKLGivggECTPDGKFSLDACRCVGACGLAPVMMVNDE--VYGRLTVDDIDD 158
Cdd:COG3411    2 SKYRHHVLVCTGS-CGAAGAEEVLDALKEELK----ERGLDGDVRVTKTGCLGRCEQGPVVVVYPEgvWYGNVTPEDVPE 76


                 ....*
gi 490745974 159 ILAKY 163
Cdd:COG3411   77 IVEEH 81
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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