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Conserved domains on  [gi|490450071|ref|WP_004320962|]
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MULTISPECIES: family 43 glycosylhydrolase [Bacteroides]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH43_FsAxh1-like cd09001
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 ...
 45-316 1.16e-134

Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase; This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


:

Pssm-ID: 350115 [Multi-domain]  Cd Length: 270  Bit Score: 396.12  E-value: 1.16e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  45 TYTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSITFQPQYRMENEKTAYSRLCWAPT 124
Cdd:cd09001    1 TYTNPVLWADYPDPDVIRVGDTYYMVSSTMHFSPGAPILHSKDLVNWEIVGYVVDRLDDGDAYYLEDGKNAYGKGIWAPS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 125 IRYDEGIYYIGVNIADDGFVMFKSTRPEGPYTMHKFEKR-LYDPGFFIDDDGKKYVTHGKGKIYLTRLKDDATGVLdpqD 203
Cdd:cd09001   81 LRYHNGKFYVYFCTNTGGTYVYTADDPAGPWSRPALIGKgYHDPSLLFDDDGKAYLVYGNGEIRLTELSPDGTGVG---G 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 204 KGTLIITAPEGYGHLfEGCHTYKRNGWYYVFNPA-LGYDGVQMISRSRNLYGPYETKVLIDDDINYAGAGVHQGGYIETA 282
Cdd:cd09001  158 EGRVIIDGTEEGLGA-EGSHLYKINGYYYIFNIEwGGGGRTQVVLRSKSLYGPYEGRVVLDDGSGTGDNGPHQGGLVDTP 236

                        250       260       270
                 ....*....|....*....|....*....|....
gi 490450071 283 EGESWAYTFQDRDYMGRCLMLYPMKWENEWPVVG 316
Cdd:cd09001  237 DGEWWFMLFQDRGAVGRIPVLVPVTWKDGWPVIG 270
GH43_C2 pfam17851
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
 345-534 5.27e-36

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


:

Pssm-ID: 436093  Cd Length: 203  Bit Score: 134.32  E-value: 5.27e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  345 SDSFDKPELAPVWEFNHVPYKEKW-SLTDRPGYFRIYAQHAKGFYWARNSLTQKVTGPYSTGTVLLDLSGLKEGDFAGNG 423
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTERPGYLRLYGRESLSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEEAGLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  424 IMG--RMMYQFGVRK-KDNRFWLEMRKGNRNAAEMIVDSLEL---KDVQRIYLRTETTKEGAtRFHYSLDNRQYHRFGPE 497
Cdd:pfam17851  81 VYYneYNHYYLGVTKdEDGGRVLRLVRCDNGELTEELAEEEVplgGEVKTVYLRVEVDGDTY-QFSYSYDGKDWKTIGPE 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 490450071  498 G--------VSNFWGFLGIRHALCCYNVMKGNPcGYADFDSFELE 534
Cdd:pfam17851 160 LdasilsdeYAAGGGFTGAFVGLYATDNGKGSS-GYADFDWFEYE 203
CBM6_xylanase-like cd04084
Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 ...
 571-667 4.82e-18

Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains; This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.


:

Pssm-ID: 271150  Cd Length: 123  Bit Score: 80.36  E-value: 4.82e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 571 QFLTDIKDSDWLVFNNLTFKKRPGKITFELQALNPGGVLEMRRGSLQGELLASCTIQSTNG--EWTKQSFEVKKLRKKEK 648
Cdd:cd04084   24 VYVGAIDNGDWIAFKNVDFGSGATSFTARVASAGAGGTIEVRLDSPDGPLIGTLEVPNTGGwqTWTTVSAPVTGVTGVHD 103

                         90       100
                 ....*....|....*....|
gi 490450071 649 VYFVF-GGSNKSLSIKNFIF 667
Cdd:cd04084  104 LYLVFkGGGGDLFNLDWFQF 123
 
Name Accession Description Interval E-value
GH43_FsAxh1-like cd09001
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 ...
 45-316 1.16e-134

Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase; This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350115 [Multi-domain]  Cd Length: 270  Bit Score: 396.12  E-value: 1.16e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  45 TYTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSITFQPQYRMENEKTAYSRLCWAPT 124
Cdd:cd09001    1 TYTNPVLWADYPDPDVIRVGDTYYMVSSTMHFSPGAPILHSKDLVNWEIVGYVVDRLDDGDAYYLEDGKNAYGKGIWAPS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 125 IRYDEGIYYIGVNIADDGFVMFKSTRPEGPYTMHKFEKR-LYDPGFFIDDDGKKYVTHGKGKIYLTRLKDDATGVLdpqD 203
Cdd:cd09001   81 LRYHNGKFYVYFCTNTGGTYVYTADDPAGPWSRPALIGKgYHDPSLLFDDDGKAYLVYGNGEIRLTELSPDGTGVG---G 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 204 KGTLIITAPEGYGHLfEGCHTYKRNGWYYVFNPA-LGYDGVQMISRSRNLYGPYETKVLIDDDINYAGAGVHQGGYIETA 282
Cdd:cd09001  158 EGRVIIDGTEEGLGA-EGSHLYKINGYYYIFNIEwGGGGRTQVVLRSKSLYGPYEGRVVLDDGSGTGDNGPHQGGLVDTP 236

                        250       260       270
                 ....*....|....*....|....*....|....
gi 490450071 283 EGESWAYTFQDRDYMGRCLMLYPMKWENEWPVVG 316
Cdd:cd09001  237 DGEWWFMLFQDRGAVGRIPVLVPVTWKDGWPVIG 270
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
35-382 1.04e-79

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 257.18  E-value: 1.04e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  35 VLLSSDNGDGTYTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSitfQPQYRmenekT 114
Cdd:COG3507   11 LLAAAAALGNTYTNPVLPGDYPDPSIIRVGDTYYLYGTSFEYFPGLPIFHSKDLVNWELVGHALDR---LPQWA-----D 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 115 AYSRLCWAPTIRYDEGIYYIGVNIADDGFV-----MFKSTRPEGPYTMH----KFEKRLYDPGFFIDDDGKKYVTHG--K 183
Cdd:COG3507   83 PYSGGIWAPDIRYHNGKYYLYYTAVDGGKNrsgigVATADDPEGPWSDPgplvCPGGNGIDPSVFVDDDGKAYLVYGsgG 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 184 GKIYLTRLKDDATGVLDPqdkGTLIITApeGYGHLFEGCHTYKRNGWYYVFNPALGYDGV---QMISRSRNLYGPYE--- 257
Cdd:COG3507  163 GGIYVAELDPDTGKLLGE---PKTLAPG--GEGGWIEGPHIYKRNGYYYLFYSEGGTCNSgyaVRVARSKSPTGPYEdap 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 258 TKVLIDDDINYAGAGVHQGGYIETAEGESWAYTFQDR--DYMGRCLMLYPMKW-ENEWPVVGpegrPGKGVVTYRKPAVk 334
Cdd:COG3507  238 GNPILTQRSDGGIQGPGHGSLVETPDGEWYLVYHAYRppGGLGRETFLDPVTWnEDGWPVVG----PGTGEPPQPLPAP- 312

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*...
gi 490450071 335 gkhkmnyphHSDSFDKPeLAPVWEFnhvpykekwsltdrpGYFRIYAQ 382
Cdd:COG3507  313 ---------ESDDFDGP-LGLQWSL---------------GYLRLTRQ 335
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
 46-313 1.73e-53

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 185.22  E-value: 1.73e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071   46 YTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSitfQPQYRMENektaySRLCWAPTI 125
Cdd:pfam04616   1 YRNPVLPGFYPDPSILRVGDDYYLTTSSFEWFPGIPIFHSKDLVNWKLVGPVLVR---RSQLSGRG-----SNASWAPDI 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  126 RYDEGIYYIGVNIADDGFVMFKSTRPEGPYT---MHKFEKRLYDPGFFIDDDGKKYVT-------HGKGKIYLTRLKDDA 195
Cdd:pfam04616  73 SYHDGKYYLYYTAVAHGIFVATADSPDGPWSdpgKLKSGGGGIDPSLFHDDDGKKYLVwggwdprHGHGGIYLQELDNDG 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  196 TGVLDPqdkGTLIITAPEGY--GHLFEGCHTYKRNGWYYVFNPALGYDG--VQMISRSRNLYGPYETK----VLIDDDIN 267
Cdd:pfam04616 153 LKLVGP---VTKLIYPGTRWvgGKVTEGPHLYKRNGYYYLTYAAGGTGGpyAVGVARSRSPLGPYEWHpgnpILTSRSPE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 490450071  268 YAGAGVHQGGYIETAEGESW-AY----TFQDRDYMGRCLMLYPMKW-ENEWP 313
Cdd:pfam04616 230 NPIYGPGHASLVETPDGEWWiVYhagrPGDGGYGLGRETRIQPVEWrADGWP 281
GH43_C2 pfam17851
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
 345-534 5.27e-36

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


Pssm-ID: 436093  Cd Length: 203  Bit Score: 134.32  E-value: 5.27e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  345 SDSFDKPELAPVWEFNHVPYKEKW-SLTDRPGYFRIYAQHAKGFYWARNSLTQKVTGPYSTGTVLLDLSGLKEGDFAGNG 423
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTERPGYLRLYGRESLSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEEAGLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  424 IMG--RMMYQFGVRK-KDNRFWLEMRKGNRNAAEMIVDSLEL---KDVQRIYLRTETTKEGAtRFHYSLDNRQYHRFGPE 497
Cdd:pfam17851  81 VYYneYNHYYLGVTKdEDGGRVLRLVRCDNGELTEELAEEEVplgGEVKTVYLRVEVDGDTY-QFSYSYDGKDWKTIGPE 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 490450071  498 G--------VSNFWGFLGIRHALCCYNVMKGNPcGYADFDSFELE 534
Cdd:pfam17851 160 LdasilsdeYAAGGGFTGAFVGLYATDNGKGSS-GYADFDWFEYE 203
CBM6_xylanase-like cd04084
Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 ...
 571-667 4.82e-18

Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains; This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.


Pssm-ID: 271150  Cd Length: 123  Bit Score: 80.36  E-value: 4.82e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 571 QFLTDIKDSDWLVFNNLTFKKRPGKITFELQALNPGGVLEMRRGSLQGELLASCTIQSTNG--EWTKQSFEVKKLRKKEK 648
Cdd:cd04084   24 VYVGAIDNGDWIAFKNVDFGSGATSFTARVASAGAGGTIEVRLDSPDGPLIGTLEVPNTGGwqTWTTVSAPVTGVTGVHD 103

                         90       100
                 ....*....|....*....|
gi 490450071 649 VYFVF-GGSNKSLSIKNFIF 667
Cdd:cd04084  104 LYLVFkGGGGDLFNLDWFQF 123
CBD_IV smart00606
Cellulose Binding Domain Type IV;
541-667 1.35e-07

Cellulose Binding Domain Type IV;


Pssm-ID: 128869 [Multi-domain]  Cd Length: 129  Bit Score: 50.79  E-value: 1.35e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071   541 HYDAFTEIDFFRYDDREGMTLVRPVGKRPMQFLTDIKDSDWLVFNNLTFKkRPGKITFELQ--ALNPGGVLEMRRGSLQG 618
Cdd:smart00606   1 GRDPYNAIQAESYDSQSGVQTETTSDAGGGKNVGYIDDGDWIAYKDVDFG-SSGAYTFTARvaSGNAGGSIELRLDSPTG 79

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|.
gi 490450071   619 ELLASCTIQSTNG--EWTKQSFEVKKLRKKEKVYFVFGGSNKsLSIKNFIF 667
Cdd:smart00606  80 TLVGTVDVPSTGGwqTYQTVSATVTLPAGVHDVYLVFKGGNY-FNIDWFRF 129
CBM_6 pfam03422
Carbohydrate binding module (family 6);
573-668 8.77e-06

Carbohydrate binding module (family 6);


Pssm-ID: 397476  Cd Length: 125  Bit Score: 45.42  E-value: 8.77e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  573 LTDIKDSDWLVFNNLTFKKRpGKITFELQALN--PGGVLEMRRGSLQGELLASCTIQSTNG--EWTKQSFEVKKLRKKEK 648
Cdd:pfam03422  25 VGYIDNGDWIAYKDVDFGSG-GAYTFTARVASgaGGGSIELRLDSPTGTLIGTVSVPSTGGwqTYVTVSANVTLPTGVHD 103

                          90       100
                  ....*....|....*....|.
gi 490450071  649 VYFVFGGSNKSLS-IKNFIFE 668
Cdd:pfam03422 104 LYLVFTGGGGYLFnIDWFQFT 124
 
Name Accession Description Interval E-value
GH43_FsAxh1-like cd09001
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 ...
 45-316 1.16e-134

Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase; This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350115 [Multi-domain]  Cd Length: 270  Bit Score: 396.12  E-value: 1.16e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  45 TYTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSITFQPQYRMENEKTAYSRLCWAPT 124
Cdd:cd09001    1 TYTNPVLWADYPDPDVIRVGDTYYMVSSTMHFSPGAPILHSKDLVNWEIVGYVVDRLDDGDAYYLEDGKNAYGKGIWAPS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 125 IRYDEGIYYIGVNIADDGFVMFKSTRPEGPYTMHKFEKR-LYDPGFFIDDDGKKYVTHGKGKIYLTRLKDDATGVLdpqD 203
Cdd:cd09001   81 LRYHNGKFYVYFCTNTGGTYVYTADDPAGPWSRPALIGKgYHDPSLLFDDDGKAYLVYGNGEIRLTELSPDGTGVG---G 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 204 KGTLIITAPEGYGHLfEGCHTYKRNGWYYVFNPA-LGYDGVQMISRSRNLYGPYETKVLIDDDINYAGAGVHQGGYIETA 282
Cdd:cd09001  158 EGRVIIDGTEEGLGA-EGSHLYKINGYYYIFNIEwGGGGRTQVVLRSKSLYGPYEGRVVLDDGSGTGDNGPHQGGLVDTP 236

                        250       260       270
                 ....*....|....*....|....*....|....
gi 490450071 283 EGESWAYTFQDRDYMGRCLMLYPMKWENEWPVVG 316
Cdd:cd09001  237 DGEWWFMLFQDRGAVGRIPVLVPVTWKDGWPVIG 270
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
35-382 1.04e-79

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 257.18  E-value: 1.04e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  35 VLLSSDNGDGTYTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSitfQPQYRmenekT 114
Cdd:COG3507   11 LLAAAAALGNTYTNPVLPGDYPDPSIIRVGDTYYLYGTSFEYFPGLPIFHSKDLVNWELVGHALDR---LPQWA-----D 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 115 AYSRLCWAPTIRYDEGIYYIGVNIADDGFV-----MFKSTRPEGPYTMH----KFEKRLYDPGFFIDDDGKKYVTHG--K 183
Cdd:COG3507   83 PYSGGIWAPDIRYHNGKYYLYYTAVDGGKNrsgigVATADDPEGPWSDPgplvCPGGNGIDPSVFVDDDGKAYLVYGsgG 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 184 GKIYLTRLKDDATGVLDPqdkGTLIITApeGYGHLFEGCHTYKRNGWYYVFNPALGYDGV---QMISRSRNLYGPYE--- 257
Cdd:COG3507  163 GGIYVAELDPDTGKLLGE---PKTLAPG--GEGGWIEGPHIYKRNGYYYLFYSEGGTCNSgyaVRVARSKSPTGPYEdap 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 258 TKVLIDDDINYAGAGVHQGGYIETAEGESWAYTFQDR--DYMGRCLMLYPMKW-ENEWPVVGpegrPGKGVVTYRKPAVk 334
Cdd:COG3507  238 GNPILTQRSDGGIQGPGHGSLVETPDGEWYLVYHAYRppGGLGRETFLDPVTWnEDGWPVVG----PGTGEPPQPLPAP- 312

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*...
gi 490450071 335 gkhkmnyphHSDSFDKPeLAPVWEFnhvpykekwsltdrpGYFRIYAQ 382
Cdd:COG3507  313 ---------ESDDFDGP-LGLQWSL---------------GYLRLTRQ 335
GH43_XYL-like cd08989
Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl ...
 48-308 1.97e-55

Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium beta-D-xylosidase SXA. These are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. It also includes various GH43 family GH43 arabinofuranosidases (EC 3.2.1.55) including Humicola insolens alpha-L-arabinofuranosidase AXHd3, Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB), and the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350103 [Multi-domain]  Cd Length: 272  Bit Score: 189.88  E-value: 1.97e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAydsITFQPQYRMENekTAYSRLCWAPTIRY 127
Cdd:cd08989    1 NPVLPGFHPDPSVVRVGDDYYMVNSTFQYFPGIPISHSKDLVHWTPIGHA---LTRPEQLDLTG--GPDGGGIWAPDISY 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 DEGIYYI-GVNIADDGF------VMFKSTRPEGPYTM-HKFEKRLYDPGFFIDDDGKKYVTHGKGKIYLTRLKDDATGVL 199
Cdd:cd08989   76 HDGKFYIyYTVVLNVGSwkgrrnYLVTSEDPEGPWSEpVWLDEGGIDPSLFVDDDGKHYMLLNPGGIRLAELNPDCTKQI 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 200 DPQ---DKGTLIITApegyghlfEGCHTYKRNGWYYVF--NPALGYDGVQMISRSRNLYGPYE----TKVLIDDDINYAG 270
Cdd:cd08989  156 GEPkriWEGTGGRAP--------EGPHLYKKDGYYYLLtaEGGTGYGHAITIARSKTIYGPYEpcpyNPILRQQDPQAPL 227

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....
gi 490450071 271 AGVHQGGYIETAEGESWAYTFQDRDY------MGRCLMLYPMKW 308
Cdd:cd08989  228 QRCGHGKLVETPDGEWWMVYLCGRPLpggycpLGRETALAPVEW 271
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
 46-313 1.73e-53

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 185.22  E-value: 1.73e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071   46 YTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSitfQPQYRMENektaySRLCWAPTI 125
Cdd:pfam04616   1 YRNPVLPGFYPDPSILRVGDDYYLTTSSFEWFPGIPIFHSKDLVNWKLVGPVLVR---RSQLSGRG-----SNASWAPDI 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  126 RYDEGIYYIGVNIADDGFVMFKSTRPEGPYT---MHKFEKRLYDPGFFIDDDGKKYVT-------HGKGKIYLTRLKDDA 195
Cdd:pfam04616  73 SYHDGKYYLYYTAVAHGIFVATADSPDGPWSdpgKLKSGGGGIDPSLFHDDDGKKYLVwggwdprHGHGGIYLQELDNDG 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  196 TGVLDPqdkGTLIITAPEGY--GHLFEGCHTYKRNGWYYVFNPALGYDG--VQMISRSRNLYGPYETK----VLIDDDIN 267
Cdd:pfam04616 153 LKLVGP---VTKLIYPGTRWvgGKVTEGPHLYKRNGYYYLTYAAGGTGGpyAVGVARSRSPLGPYEWHpgnpILTSRSPE 229

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 490450071  268 YAGAGVHQGGYIETAEGESW-AY----TFQDRDYMGRCLMLYPMKW-ENEWP 313
Cdd:pfam04616 230 NPIYGPGHASLVETPDGEWWiVYhagrPGDGGYGLGRETRIQPVEWrADGWP 281
GH43_XynB-like cd18617
Glycosyl hydrolase family 43, such as Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, ...
 48-315 5.00e-53

Glycosyl hydrolase family 43, such as Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB); This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been characterized to have alpha-L-arabinofuranosidase (EC 3.2.1.55) and beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activities. Beta-1,4-xylosidases are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Also included in this subfamily are Bacteroides ovatus alpha-L-arabinofuranosidases, BoGH43A and BoGH43B, both having a two-domain architecture, consisting of an N-terminal 5-bladed beta-propeller domain harboring the catalytic active site, and a C-terminal beta-sandwich domain. However, despite significant functional overlap between these two enzymes, BoGH43A and BoGH43B share just 41% sequence identity. The latter appears to be significantly less active on the same substrates, suggesting that these paralogs may play subtly different roles during the degradation of xyloglucans from different sources, or may function most optimally at different stages in the catabolism of xyloglucan oligosaccharides (XyGOs), for example before or after hydrolysis of certain side-chain moieties. It also includes Phanerochaete chrysosporium BKM-F-1767 Xyl, a bifunctional xylosidase/arabinofuranosidase. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350129 [Multi-domain]  Cd Length: 285  Bit Score: 183.86  E-value: 5.00e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAydsITFQPQYRMENEKTaySRLCWAPTIRY 127
Cdd:cd18617    1 NPILPGFYPDPSICRVGDDYYLVTSSFEYFPGLPIYHSKDLVNWELIGHA---LDRPSQLDLRGVPS--SGGIFAPTIRY 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 DEGIYYI---GVNIADDGFVMFKSTRPEGPYT-MHKFEKRLYDPGFFIDDDGKKYVTH---------GKGKIYLTRLkDD 194
Cdd:cd18617   76 HDGRFYIittNVSTDGRGNFIVTADDPAGPWSdPVWLDGPGIDPSLFFDDDGKVYLTGtgpppdpyeGHGGIWQQEI-DL 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 195 ATGVLdpQDKGTLIITAPEGYGHLfEGCHTYKRNGWYYVFNpALG---YDGVQMISRSRNLYGPYETK----VL--IDDD 265
Cdd:cd18617  155 ETGKL--LGEPKVLWNGGTGGRWP-EGPHLYKIDGWYYLLI-AEGgteEGHSETIARSRSPWGPYEPCpnnpILthRHLG 230

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 266 INYagagVHQGGY---IETAEGESWAyTF-------QDRDYMGRCLMLYPMKWENEWPVV 315
Cdd:cd18617  231 SNP----VQNTGHadlVEDPDGSWWA-VFlgvrpygGGFHNLGRETFLAPVEWEDGWPVV 285
GH43_PcXyl-like cd18833
Glycosyl hydrolase family 43 protein such as the bifunctional Phanerochaete chrysosporium ...
 48-314 5.81e-37

Glycosyl hydrolase family 43 protein such as the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl); This glycosyl hydrolase family 43 (GH43) subgroup includes Phanerochaete chrysosporium BKM-F-1767 Xyl, a characterized bifunctional enzyme with beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37)/ alpha-L-arabinofuranosidase (EC 3.2.1.55) activities. This subgroup belongs to the GH43_XybB subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_XybB subgroup includes enzymes having beta-1,4-xylosidase and alpha-L-arabinofuranosidase activities. Beta-1,4-xylosidases are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43_XybB subgroup includes Bacteroides ovatus alpha-L-arabinofuranosidases, BoGH43A and BoGH43B, both having a two-domain architecture, consisting of an N-terminal 5-bladed beta-propeller domain harboring the catalytic active site, and a C-terminal beta-sandwich domain. However, despite significant functional overlap between these two enzymes, BoGH43A and BoGH43B share just 41% sequence identity. The latter appears to be significantly less active on the same substrates, suggesting that these paralogs may play subtly different roles during the degradation of xyloglucans from different sources, or may function most optimally at different stages in the catabolism of xyloglucan oligosaccharides (XyGOs), for example before or after hydrolysis of certain side-chain moieties. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350154  Cd Length: 292  Bit Score: 140.07  E-value: 5.81e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRVGED---YYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDSITFQPQYrmENEKTAYSRLCWAPT 124
Cdd:cd18833    1 NPIIPGFHPDPSCIFVPEWdgtFFCVTSSFLAFPGIPIYASKDLINWKLISNVLSRPSQLPEL--ATTGTGQQGGIWAPT 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 125 IRYDEGIYYIGVNIADD--------GFVMFKSTrpeGPYTMH------KFEKRLYDPGFFIDDDGKKYVT-----HGKGK 185
Cdd:cd18833   79 LRYHDGTFYVITTLVFPdktdasrwDNLLFTTT---DPYSDSawsdpiRFDFPGYDPDLFWDDDGTAYVQgahywRVRPE 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 186 IYLTRLkDDATGvldpqdKGTLIITAPEGYGHLF-EGCHTYKRNGWYY--VFNPALGYDGVQMISRSRNLYGPYEtkvli 262
Cdd:cd18833  156 IQQQEI-DLKTG------ESLSPSPIWNGTGGSApEGPHMYKKDGWYYllIAEGGTGLGHSVTIARSRSIWGPYE----- 223

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490450071 263 dddiNYAG------AGVHQggYIET---------AEGESWAY---TFQDRDY----MGRCLMLYPMKW-ENEWPV 314
Cdd:cd18833  224 ----SYPSnpvltnANTSE--YFQTvghadlfqdANGNWWGValaTRSGPEYeiypMGRETVLYPVTWeEGEWPV 292
GH43_C2 pfam17851
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
 345-534 5.27e-36

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


Pssm-ID: 436093  Cd Length: 203  Bit Score: 134.32  E-value: 5.27e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  345 SDSFDKPELAPVWEFNHVPYKEKW-SLTDRPGYFRIYAQHAKGFYWARNSLTQKVTGPYSTGTVLLDLSGLKEGDFAGNG 423
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTERPGYLRLYGRESLSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEEAGLV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  424 IMG--RMMYQFGVRK-KDNRFWLEMRKGNRNAAEMIVDSLEL---KDVQRIYLRTETTKEGAtRFHYSLDNRQYHRFGPE 497
Cdd:pfam17851  81 VYYneYNHYYLGVTKdEDGGRVLRLVRCDNGELTEELAEEEVplgGEVKTVYLRVEVDGDTY-QFSYSYDGKDWKTIGPE 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 490450071  498 G--------VSNFWGFLGIRHALCCYNVMKGNPcGYADFDSFELE 534
Cdd:pfam17851 160 LdasilsdeYAAGGGFTGAFVGLYATDNGKGSS-GYADFDWFEYE 203
GH43_SXA-like cd09000
Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This ...
 48-315 6.33e-33

Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized to mainly have beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium (Xsa;Sxa;SXA), Bifidobacterium adolescentis ATCC 15703 (XylC;XynB;BAD_0428) and Bacillus sp. KK-1 XylB. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. These enzymes possess an additional C-terminal beta-sandwich domain that restricts access for substrates to a portion of the active site to form a pocket. The active-site pockets comprise of two subsites, with binding capacity for two monosaccharide moieties and a single route of access for small molecules such as substrate. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350114 [Multi-domain]  Cd Length: 292  Bit Score: 128.43  E-value: 6.33e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAydsITFQPQYRMENEKTAYSrlCWAPTIRY 127
Cdd:cd09000    1 NPILPGFNPDPSICRVGDDYYIATSTFEWFPGVQIHHSKDLVNWELVARP---LTRVSQLDMRGNPDSGG--IWAPCLSY 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 DEGIYYI---GVNIAD------DGFVMfKSTRPEGPYTMHKFekrL----YDPGFFIDDDGKKYV----------THGKG 184
Cdd:cd09000   76 ADGKFWLvytDVKSVDgpfkdvHNYLV-TAESIEGPWSEPIY---LnssgFDPSLFHDDDGRKYLvnmlwdhrpgHNRFA 151

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 185 KIYLTRLkDDATGVLDPQDKgtlIITAPEGYGhLFEGCHTYKRNGWYYVFNPA--LGYDGVQMISRSRNLYGPYET---K 259
Cdd:cd09000  152 GIVLQEF-DPETKKLVGERK---VIFKGTELG-LTEGPHLYKRDGYYYLLTAEggTGYEHAVTVARSRNIFGPYEVdpdN 226

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 490450071 260 VLI---DDDINY---AGagvHqGGYIETAEGEsWAYTF--------QDRDYMGRCLMLYPMKWENE-WPVV 315
Cdd:cd09000  227 PLLtswDDPENPlqkAG---H-GSLVETPDGE-WYLAHlcgrplpgRGRCPLGRETAIQKVEWTDDgWPRL 292
GH43_XYL-like cd09002
Glycosyl hydrolase family 43, beta-D-xylosidase (uncharacterized); This glycosyl hydrolase ...
 46-315 3.24e-31

Glycosyl hydrolase family 43, beta-D-xylosidase (uncharacterized); This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350116 [Multi-domain]  Cd Length: 271  Bit Score: 123.11  E-value: 3.24e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  46 YTNPVINADYPDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDsitfqpqyrmENEKTAysrlcWAPTI 125
Cdd:cd09002    1 YLNPILAGDYPDPSILRDGDDYYMTHSSFDYYPGLLIWHSRDLVNWEPIGAALT----------EYIGTV-----WAPDL 65

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 126 RYDEGIYYIGVNIADDGFVMFKSTRPEGPYT--MHKFEKRLYDPGFFIDDDGKKYVTHGKGKIYltRLKDDATGVLDPQD 203
Cdd:cd09002   66 IKHDGRYYIYFPAKGGTNYVITADDIAGPWSepIDLKVGSGIDPGHVVDEDGKRYLFLSGGRRV--RLTDDGLSVAGPPE 143

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 204 KgtliITAPEGY-------GHLFEGCHTYKRNGWYYVFN-------PALGYdgvqMI--SRSRNLYGPYE---------T 258
Cdd:cd09002  144 K----VYDGWRYpdewdveCFCLEGPKLFRRGGYYYLTTaqggtagPPTSH----MVvsARSKSPHGPWEnspynplvrT 215

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490450071 259 KvliDDDINYAGAGvHqGGYIETAEGESW----AYTfQDRDYMGRCLMLYPMKWENE-WPVV 315
Cdd:cd09002  216 Q---SREEKWWSKG-H-GTLVEGPDGKWWmvyhGYE-NGYRTLGRQTLLEPVEWTADgWFRI 271
GH43_ABN-like cd08999
Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase ...
 48-314 1.11e-29

Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350113 [Multi-domain]  Cd Length: 284  Bit Score: 118.79  E-value: 1.11e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRVGEDYYMVSSSFVAmPGIPVCHSKDLINWQIIGHayDSITFQPQYRMENEKTaysrlcWAPTIRY 127
Cdd:cd08999    1 NPVIDGDFPDPSVIRVGGTYYAFATNSGG-KNVQVATSTDLVTWTLLGG--DALPDLPAWAAAGGNT------WAPDVVR 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 degiyyigvnIADDGFVMFKSTR----------------PEGPYTMHkfEKRLY---------DPGFFIDDDGKKYVTHG 182
Cdd:cd08999   72 ----------RPDGKYVMYYSARlkssgkhcigvatsdsPLGPFTPV--GEPPLcpldqggaiDPSGFVDPDGKRYLVYK 139

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 183 -----KGK---IYLTRLKDDATGVLDpqDKGTLIITAPEGYGHLFEGCHTYKRNGWYYVFNPALGYDGVQ---MISRSRN 251
Cdd:cd08999  140 vdgnsIGVptpIMLQELSADGLTLVG--EPVELLLNDGPWDGPLVEAPSLVKRDGTYYLFYSSNCYCSPSyavGYATSKS 217

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 252 LYGPYE---TKVLIDDDINYAGAGvhqGGYIETAEGESW----AYTFQDRDYMGRCLMLYPMKWENEWPV 314
Cdd:cd08999  218 ITGPYTkagEPLLLTGDGGLTGPG---GADVVEDDGGDWmvfhAWDGGDDVGGGRAMYTAELTWEGGWPV 284
GH43_ABN-like cd18616
Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl ...
 48-308 2.30e-23

Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activity. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350128 [Multi-domain]  Cd Length: 291  Bit Score: 100.73  E-value: 2.30e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  48 NPVINADYPDPDIIRvGED--YYMVSSSFVAMPG-----IPVCHSKDLINWQIIGHAYDSitfQPQYRmeNEKTAYsrlC 120
Cdd:cd18616    1 NPVFEPTFADPTVIR-GDDgyFYAYATEDPWGDGggfrlVPILRSKDLVNWEYVGDAFTS---KPRWK--WDPGGG---L 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 121 WAPTIRYDEGIYY---------------IGVNIADdgfvmfkstRPEGPYTMH--KFEKRLY------DPgFFIDDDGKK 177
Cdd:cd18616   72 WAPDIRYIDGKYVlyyslsdwgadpnpgIGVATAD---------SPAGPFTDQgkLFDSNEIgvrnsiDP-FVFEDDGKK 141

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 178 YVTHGKGK-IYLTRLKDDATGVldpqDKGTLIITApegyGHLFEGCHTYKRNGWYYVF--------NPALGYdGVqMISR 248
Cdd:cd18616  142 YLFWGSFYgIYAVELTADGLAL----KPGEKVQIA----GDRYEGPYIVKRDGYYYLFgsagscceGPNSTY-RV-VVGR 211

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 249 SRNLYGPYETK--------------VLIDDDINYAGAGvHqGGYIETAEGESW----AYTFQDRDYMG----RCLMLYPM 306
Cdd:cd18616  212 SESLLGPYVDRdgrslldsggggtpVVLQNGNRFVGPG-H-NAVITDDAGQDWmlyhAYDRNDPYLPGgynrRPLLLDRL 289


                 ..
gi 490450071 307 KW 308
Cdd:cd18616  290 DW 291
GH43_Arb43a-like cd08998
Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 ...
 57-276 4.49e-23

Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase Arb43A; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350112 [Multi-domain]  Cd Length: 278  Bit Score: 99.54  E-value: 4.49e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGED-YYMVSSSfvamPGIPVCHSKDLINWQIIGHAYDSITFQPQYRMENEKTAYsrlcWAPTIRYDEGIYY-- 133
Cdd:cd08998    3 DPSIIKDDGGtYYVFSTG----AGIQIRTSKDLVNWEFVGTVFPEGPAWAAAEVPGGAGGL----WAPDVVYVNGRYYly 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 134 ------------IGVNIA---------DDGFVmFKSTRPEGPYTMhkfekrlyDPGFFIDDDGKKYVTHG--KGKIYLTR 190
Cdd:cd08998   75 ysastfgsnrsaIGLATSttlddgpwtDQGLV-VSSSPGDDYNAI--------DPNVFVDADGRLWLAYGsfWGGIKLVE 145

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 191 LkDDATGVLDPQDKGTLIITAPeGYGHLFEGCHTYKRNGWYYVFnpaLGYD----GVQ-----MISRSRNLYGPY----- 256
Cdd:cd08998  146 L-DPATGKLRPGSTGTSIASRP-GGPGAIEAPYIIYRGGYYYLF---VSYGsccrGANstyniRVGRSTSITGPYvdrng 220

                        250       260
                 ....*....|....*....|....*.
gi 490450071 257 ------ETKVLIDDDINYAGAGvHQG 276
Cdd:cd08998  221 vdmlegGGTLLLGGHGRWIGPG-HNS 245
GH43-like cd08986
Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43) ...
 57-256 8.33e-21

Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350100 [Multi-domain]  Cd Length: 257  Bit Score: 92.29  E-value: 8.33e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIrVGED--YYMVSSsfVAMP-------GIPVCHSKDLINWQIIGHAYD---SITFQPQYRMENEKTAYSRLCWAPT 124
Cdd:cd08986    4 DPYIT-LGPDgyYYLTGT--TGGPdwwgvndGIRLWRSKDLKDWEYLGLVWDlekDGWWQWEPQWWTPDSKNKRALWAPE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 125 IRYDEGIYYI--GVNIADDGFVMFKSTRPEGPYT-MHK--FEKRlYDPGFFIDDDGKKYVTHGKGKIylTRLKDDATGVl 199
Cdd:cd08986   81 IHYINGTWYIthSMNGGGTGLLKSTTGKPEGPYVdPMGgpLGKG-IDPSLFEDDDGTVYLVWGNGQI--ARLKKDMSGF- 156

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 490450071 200 dPQDKGTLIITAPEGYGHlfEGCHTYKRNGWYYVFNPA-LGYDGVQ-----MISRSRNLYGPY 256
Cdd:cd08986  157 -AEEPRKIDPSGNREIGH--EGAFIFKIGGKYVLFGAAwSTDKMRKgtydlYYATSDSIYGPY 216
GH_F cd08978
Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F ...
 56-257 7.07e-20

Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350092 [Multi-domain]  Cd Length: 251  Bit Score: 89.42  E-value: 7.07e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  56 PDPDIIRVGEDYYMVSSS--FVAMPGIPVCHSKDLINWQIIGHAydsitfqPQYRMENEKTaySRLCWAPTIRYDE-GIY 132
Cdd:cd08978    1 ADPSILKDNGRYYIYATTddTGTGTGIVVWKSKDLVNWKEEGTV-------LSRGKSKSWG--TGNLWAPEVYYFNsGKW 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 133 Y----IGVNIADDGFVMFKSTRPEGPYTMH------KFEKRLYDPGFFIDDDGKKYVTHGK----GKIYLTRL-KDDATG 197
Cdd:cd08978   72 YlyysAVPNGGGGRIYVATSDSPEGPFTPIvsgklgDRGSGSIDPTVFVDDDGKLYLYYGDeddsGDIYVAELdPDLLTI 151

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490450071 198 VLDPQDKGTlIITAPEGYGHLFEGCHTYKRNGWYYVFNPALGYDGVQ--MISRSRNLYGPYE 257
Cdd:cd08978  152 KGDVTLLIG-EVVGSGFRGNYFEGPAVFKRNGYYYLIYSAGGTDGGYaiGYATSDSPLGPWE 212
GH43_ABN cd08988
Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes ...
 56-259 1.56e-18

Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350102 [Multi-domain]  Cd Length: 277  Bit Score: 86.42  E-value: 1.56e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  56 PDPDIIRVGEDYYMVSSSFVAMpGIPVCHSKDLINWQIighaYDSITFQPQYRMENEKTAYSRLCWAPTIRYDEGIYYIG 135
Cdd:cd08988    1 HDPSIIKEGGTYYAFGTGTDGF-GIPIAKSKDLGNWTI----VGEAFATLPSWKGGSPPSADGNLWAPDISQHKGKYYLY 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 136 VNIADDG-----FVMFKSTRPEGPYTMHKFEK---------RLYDPGFFIDDDGKKYVTHGK--GKIYLTRLKDDATGVL 199
Cdd:cd08988   76 YSVSDNGsntsaIGLATANNPQGPFKDEGPAKpvvtsdnagNAIDPDLFQDEDGQNWLLYGSfwGGIWLQKLDKNGLVVN 155

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 490450071 200 DPQDKGTliITApeGYGHLFEGCHTYKRNGWYYVFNPALGY-DGVQ-----MISRSRNLYGPYETK 259
Cdd:cd08988  156 PPGNGKS--IAV--LYYVSIEAPYITYAGGYYYLFVSAGSCcDGGNstyhtRVGRSKKVTGPYLDK 217
GH43_HoAraf43-like cd08991
Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 ...
 56-256 1.96e-18

Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (HoAraf43;Hore_20580); This glycosyl hydrolase family 43 (GH43) subgroup includes Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (EC 3.2.1.55) (HoAraf43;Hore_20580). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. This GH43_ HoAraf43-like subgroup includes enzymes that have been annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350105 [Multi-domain]  Cd Length: 283  Bit Score: 86.07  E-value: 1.96e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  56 PDPDIIRVGEDYYMVSSSFVAMPGIPVCHSKDLINWQIIGHAYDsitfqpqyrmenEKTAYSRLC-WAPTIRYDEGIYYI 134
Cdd:cd08991    1 ADPFVLKHNGTYYLYGTGGDDGRGFKVYVSDDLVNWEYPGGALE------------EPGLWGTKGfWAPEVFYYNGKFYM 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 135 --GVNIADDGFVMF--KSTRPEGPYT-----MHKFEKRLYDPGFFIDDDGKKYVTH--------GKGKIYLTRLKDDATG 197
Cdd:cd08991   69 yySANGGDHGEHIAvaVSDSPLGPFRdkgklLIPAGGFSIDAHVFIDDDGKWYLYYvrddlggePGNRIYVAELEDDLSL 148

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 490450071 198 VLDPQDKGTLIITAPEGYGHLF-----EGCHTYKRNGWYYVF-------NP--ALGYdgvqmiSRSRNLYGPY 256
Cdd:cd08991  149 IGEPTLVLCPTADERWEYGEGRdwhttEGPTVLKHNGTYYLTysanhfrSPdyAVGY------ATADSPLGPW 215
CBM6_xylanase-like cd04084
Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 ...
 571-667 4.82e-18

Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains; This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.


Pssm-ID: 271150  Cd Length: 123  Bit Score: 80.36  E-value: 4.82e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 571 QFLTDIKDSDWLVFNNLTFKKRPGKITFELQALNPGGVLEMRRGSLQGELLASCTIQSTNG--EWTKQSFEVKKLRKKEK 648
Cdd:cd04084   24 VYVGAIDNGDWIAFKNVDFGSGATSFTARVASAGAGGTIEVRLDSPDGPLIGTLEVPNTGGwqTWTTVSAPVTGVTGVHD 103

                         90       100
                 ....*....|....*....|
gi 490450071 649 VYFVF-GGSNKSLSIKNFIF 667
Cdd:cd04084  104 LYLVFkGGGGDLFNLDWFQF 123
GH43_CjArb43A-like cd18830
Glycosyl hydrolase family 43 protein such as Cellvibrio japonicus Ueda107 endo-alpha-1, ...
 57-297 7.88e-18

Glycosyl hydrolase family 43 protein such as Cellvibrio japonicus Ueda107 endo-alpha-1,5-L-arabinanase / exo-alpha-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A); This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes annotated with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities, and includes the bifunctional Cellvibrio japonicus Ueda107 endo-alpha-1,5-L-arabinanase / exo-alpha-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350151 [Multi-domain]  Cd Length: 291  Bit Score: 84.25  E-value: 7.88e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMvsssFVAMPGIPVCHSKDLINWQIIGHAYDSitfQPQYRMENEKTaYSRLCWAPTIRYDEGIYYI-- 134
Cdd:cd18830    3 DPVMAREGGTYYL----FSTGPGISVMSSKDLKNWTQERPVFDE---PPQWAKEAVPG-FNGHIWAPDISFHNGRYYLyy 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 135 -----GVNIADDGFVMFKSTRPEGPytMHKFEKR-----------LY---DPGFFIDDDGKKYVTHGK--GKIYLTRLKD 193
Cdd:cd18830   75 scsafGKNTSAIGVATNKTLDPDSP--DYKWEDHgmvvqsvpgrdLWnaiDPNVIVDEKGTPWLSFGSfwGGIKLVKLDP 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 194 DATGVLDPQDKGTL-------IITAPEGYGHLFEGCHTYKRNGWYYVFnpaLGYD----GVQ-----MISRSRNLYGPYE 257
Cdd:cd18830  153 DLKSLAEPQEWHTIarrertfKLTDSEAGPGAIEAPFIFKKGGYYYLF---VSWDyccrGVNstykvVVGRSKNVTGPYL 229

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|.
gi 490450071 258 TK-----------VLIDDDINYAGAGvHQGgyietaegeswAYTFQDRDYM 297
Cdd:cd18830  230 DKdgksmlqgggtLVVGGNKRWAGVG-HNS-----------VYTFDGKDYL 268
GH43_AXH_like cd08990
Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, ...
 85-280 1.73e-16

Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, beta-xylosidase, endo-1,4-beta-xylanase, and alpha-L-arabinofuranosidase; This subgroup includes Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160), Butyrivibrio proteoclasticus alpha-L-arabinofuranosidase (Xsa43E;bpr_I2319), Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and metagenomic beta-xylosidase (EC 3.2.1.37) / alpha-L-arabinofuranosidase (EC 3.2.1.55) CoXyl43. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_AXH-like subgroup includes enzymes that have been characterized with beta-xylosidase, alpha-L-arabinofuranosidase, endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Metagenomic beta-xylosidase/alpha-L-arabinofuranosidase CoXyl43 shows synergy with Trichoderma reesei cellulases and promotes plant biomass saccharification by degrading xylo-oligosaccharides, such as xylobiose and xylotriose, into the monosaccharide xylose. Studies show that the hydrolytic activity of CoXyl43 is stimulated in the presence of calcium. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350104 [Multi-domain]  Cd Length: 269  Bit Score: 79.95  E-value: 1.73e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  85 SKDLINWQIIGHAYDSITFqpqyrmeneKTAYSRLCWAPTIRYDEGIYY--IGVNIADDGF---VMfKSTRPEGPYTMHK 159
Cdd:cd08990   37 STDLVNWTDHGEILPPDDV---------FWWASGNAWAPDAVYKNGKYYfyFPVGQASDGFgigVA-VSDSPAGPFKDAL 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 160 FEK---------RLYDPGFFIDDDGKKYV-THGKGKIYLTRLKDDATGVldpqdKGTLIITAPEGYGHLFEGCHTYKRNG 229
Cdd:cd08990  107 GKPlipeglngiEGIDPAVFVDDDGRAYLyFGGGGGYYVAKLKDDMISL-----AGEPQKIKNGGLKGFFEAPWVFKRNG 181

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 490450071 230 WYYVFNPALGYDGVQM-ISRSRNLYGPYETKVLIdddINYAGAGVHQGGYIE 280
Cdd:cd08990  182 TYYLSYAGGWAYPAEIaYSTADSPLGPYTYRGVI---LDPVGSGTNHGSIVE 230
GH43_XylA-like cd18620
Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium ...
 87-291 2.16e-16

Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium alpha-L-arabinofuranosidase XylA; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. The GH43_XylA-like subgroup includes Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), endo-alpha-L-arabinanase (EC 3.2.1.-) as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan.


Pssm-ID: 350132 [Multi-domain]  Cd Length: 274  Bit Score: 79.95  E-value: 2.16e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  87 DLINWQIIGHAYDSitfqpqyRMENEKTAYSRLC-WAPTIRYDEGIYYIGVNIADDGFVMF-KSTRPEGPYT------MH 158
Cdd:cd18620   40 DLSNWRYHGVIFRS-------DQDPDEVPPGKGLlYAPDVVKGPGRYYLYYCLSKGSVEGVaVSDSPAGPFEylgpvkYP 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 159 KFEKRL-YDPGFFIDDDGKKYVTHGKGKIYLTRLKDDATGVLDPQDKGTLIITAPEGYGhLFEGCHTYKRNGWYY-VF-- 234
Cdd:cd18620  113 RKGDIFqIDPAVLVDDDGRVYLYWGQGGSKGAELDPDMLTIKPETIVDVPAGITFEGHG-FFEGSSIRKINGIYYlVYss 191

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 490450071 235 -----NPALGYdgvqMISRSRnlYGPYETK-VLID-DDINYAGAGVHqgGYIETAEGEsWaYTF 291
Cdd:cd18620  192 isrgrPTELCY----ATSKSP--LGPFTYGgVIIDnGGCDPPSGNNH--GSIVEINGQ-W-YIF 245
GH43_bXyl-like cd09004
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
 57-232 1.10e-14

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675) and (BT3662;BT_3662); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350118 [Multi-domain]  Cd Length: 266  Bit Score: 74.57  E-value: 1.10e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYY----------MVSSSFVAMpgipvcHSKDLINWQiiGHaydsitfQPQYRMENEKTAYSRLCWAPTIR 126
Cdd:cd09004    2 DPDIVVFGGRYYiypttdgppgWSSTSFHVF------SSTDLVNWT--DH-------GIILDLANDVWWANKGAWAPAVA 66

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 127 YDEGIYY--------IGVNIADdgfvmfkstRPEGPYT--------MHKFEKRLYDPGFFIDDDGKKYVTHGKGKIYLTR 190
Cdd:cd09004   67 ERNGKYYfyfsagsqIGVAVSD---------SPTGPFTdlgrplvtGGDYGGQAIDPMVFVDDDGQAYLYWGNGTAYVAR 137

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 490450071 191 LKDDATGVldpqDKGTLIITAPEGYghlFEGCHTYKRNGWYY 232
Cdd:cd09004  138 LNDDMVSF----DGEVVVSITPPNF---REGPFVHKRNGIYY 172
GH43_F5-8_typeC-like cd18608
Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 ...
 55-257 8.32e-14

Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 domain; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), xylanase (EC 3.2.1.8), and beta-galactosidase (EC 3.2.1.145) activities, and some as F5/8 type C domain (also known as the discoidin (DS) domain)-containing proteins. Most contain a F5/8 type C domain C-terminal to the GH43 domain. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350120 [Multi-domain]  Cd Length: 276  Bit Score: 72.31  E-value: 8.32e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  55 YPDPDIIRVGEDYYMVSSSFVAMP---GIPVC-HSKDLINWQiighaYDSITFQPQyrmenEKTAYSRlCWAP-TIRYDE 129
Cdd:cd18608    1 FADPSIVKFGGTYYLYATTDGWGGfnsGEPVVwKSKDFVNWK-----FEGLNWPTK-----AASGDSK-VWAPsVVKGKD 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 130 GIYYIGVNIADDGFVM--------FKSTRPEGPYTMHKFEKRLY---DPGFFIDDDGKKYVT-----HGKGKIYLTRLKD 193
Cdd:cd18608   70 GKYYMYVSVGSEIYVGvadsplgpWKNANGDGPPIIPGDGKPNYhmiDAEPFIDDDGKAYLYwgsglHVNGHCFAAKLNP 149

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 194 DATGVldpqDKGTLIITAPEGYghlFEGCHTYKRNGWYYVFNpALG------YDGVQMIsrSRNLYGPYE 257
Cdd:cd18608  150 DMVTF----DGSEPTIVTPRDY---FEAPFMFKRNGIYYLMY-SGGgcwdetYNVRYAV--SDNPLGPFE 209
GH43_XlnD-like cd18827
Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); ...
 85-287 4.16e-10

Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have mostly been annotated as xylanases (endo-alpha-L-arabinanase, EC 3.2.1.8). It belongs to the GH43_bXyl-like subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl-like subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidases (EC 3.2.1.37) and xylanases, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350148 [Multi-domain]  Cd Length: 277  Bit Score: 61.14  E-value: 4.16e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  85 SKDLINWQIIGHAYDSITFqPQYRmenektaysRLCWAPTIRYDEGIYY----------------IGVNIADdgfvmfks 148
Cdd:cd18827   33 SPDLVHWTKHERILDMADV-PWAN---------RAVWAPSVIEKNGKYYlyfaandiqsddegggIGVAVAD-------- 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 149 tRPEGPYTMHkFEKRLY----------DPGFFIDDDGKKYVTHGK-GKIYLTRLKDDATGVLDPQDKGTLIITAPEGYGh 217
Cdd:cd18827   95 -RPEGPFKDA-LGKPLIgefhngaqpiDQHVFKDDDGQAYLYYGGwGHCNVAKLNDDMTSLVPFDDGETFKEITPEGYV- 171

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 490450071 218 lfEGCHTYKRNGWYYVFNPALGYDG----VQMiSRSRNLYGPYET--KVLIDDDINYAGAGVHqgGYIETAEGESW 287
Cdd:cd18827  172 --EGPFMFKRNGKYYFMWSEGGWTGpdysVAY-AVADSPLGPFKRigKILQQDPAIATGAGHH--SVVNVPGTDDW 242
GH43_GsAbnA-like cd18832
Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1, ...
 57-256 1.56e-09

Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1,5-L-arabinanase AbnA; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. It includes Geobacillus stearothermophilus T-6 NCIMB 40222 AbnA, Bacillus subtilis subsp. subtilis str. 168 (Abn2;YxiA;J3A;BSU39330) (Arb43B), and Thermotoga petrophila RKU-1 (AbnA;TpABN;Tpet_0637). These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350153 [Multi-domain]  Cd Length: 332  Bit Score: 59.96  E-value: 1.56e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMVSSSFVAmpgipvCHSKDLINWQIIG---HAYDSITFQPQYRME-------NEKTAYSrlCWAPTIR 126
Cdd:cd18832    3 DPSIVKDDGTYYVFGSHLAA------AKSTDLMNWTQFTngvTTDNPLLFNLFDSTAwelaedfNWAGGGN--LWAPDVI 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 127 YDEG-----IYY----------IGVNIADD--------------GFVMFKSTRPEGPYTMHKFEKRLY----DPGFFIDD 173
Cdd:cd18832   75 YNKAmgkycMYYsvsgddspsaIGLATADNiegpytykgtvlksGFTGSTSADADVYLTGGKYNNNYHpnaiDPCVFYDK 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 174 DGKKYVTHGK--GKIYLTRLkDDATGVLDPQDKGTLIITAPeGYG-HLFEGCHT-----Y----KRNGWYYVFNP--ALG 239
Cdd:cd18832  155 DGKLWMVYGSwsGGIFLLEL-DPKTGLRDYSVETDGNLPDQ-YYGkKIAGGYHAsgegpYilydKDTGYYYLFVSygGLD 232

                        250
                 ....*....|....*....
gi 490450071 240 YD-GVQM-ISRSRNLYGPY 256
Cdd:cd18832  233 ANgGYNIrVFRSKNPDGPY 251
GH43_BsArb43A-like cd18829
Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 ...
 57-259 4.52e-09

Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase Arb43A; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes annotated as having endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities, and includes Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase (AbnA;BSU28810) (Arb43A). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the arabinofuranosidase (ABF; EC 3.2.1.55) enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350150 [Multi-domain]  Cd Length: 273  Bit Score: 57.76  E-value: 4.52e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMVSSSfvamPGIPVCHSKDLINWQIIGHAYDSitfQPQYRMENEKTAYSRLCWAPTIRYDEGIYY--- 133
Cdd:cd18829    3 DPSIIKEGSTWWTFSTG----DGIPVKYSSDGLNWTQGPPIFGS---PLSWWKTYVPANTTNDVWAPDVHYYNGKYWlyy 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 134 -----------IGV---------NIADDGFVMfKSTRPEGpytmhkfekrlY---DPGFFIDDDGKKYVTHGK--GKIYL 188
Cdd:cd18829   76 aistfgsntsaIGLasassiaagNWTDEGLVL-RSTSADN-----------YnaiDPNLVIDASGNPWLVFGSfwSGIKI 143

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 490450071 189 TRLkDDATgvLDPQDKGTLIITAPEGYghlFEGCHTYKRNGWYYVFnPALGY--DGVQ-----MISRSRNLYGPYETK 259
Cdd:cd18829  144 TRL-DKAT--MKPTGSIYSIASRPSGG---IEGPFIVYRDGYYYLF-VSIDKccRGVNstykiAYGRSTSITGPYLDK 214
GH43_Xsa43E-like cd18618
Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase ...
 121-264 7.60e-09

Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase Xsa43E; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. This subgroup includes Cellvibrio japonicus arabinan-specific alpha-1,2-arabinofuranosidase, CjAbf43A, which confers its specificity by a surface cleft that is complementary to the helical backbone of the polysaccharide, and Butyrivibrio proteoclasticus GH43 enzyme Xsa43E, also an arabinofuranosidase, which has been shown to cleave arabinose side chains from short segments of xylan. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350130 [Multi-domain]  Cd Length: 275  Bit Score: 57.23  E-value: 7.60e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 121 WAPTIRYDEGIYY--------------IGVNIADdgfvmfkstRPEGPYT-----------MHKFEKRLY---DPGFFID 172
Cdd:cd18618   66 WAGQVIERNGKFYwyvpvhhktnggfaIGVAVSD---------SPTGPFKdalgkplitndMTGTTNHSWddiDPTVFID 136

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 173 DDGKKYVTHGKGKIYLTRLKDDATGVldpqdKGTLIITAPEGYGHLFEGCHTYKRNGWYYV-----FNPALGYdgvqmiS 247
Cdd:cd18618  137 DDGQAYLYWGNPELYYVKLKEDMISL-----DGEIGTIDISGLPDFTEAPWVHKRNGLYYLsyaagFPEKIAY------A 205

                        170
                 ....*....|....*..
gi 490450071 248 RSRNLYGPYETKVLIDD 264
Cdd:cd18618  206 TSDSPTGPWTYKGVIMD 222
GH43_CtGH43-like cd18822
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
 60-289 4.16e-08

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43), Streptomyces avermitilis MA-4680 = NBRC 14893 (Sa1,3Gal43A;SAV2109) (1,3Gal43A), and Ruminiclostridium thermocellum ATCC 27405 (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A). It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350143  Cd Length: 266  Bit Score: 54.93  E-value: 4.16e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  60 IIRVGEDYYMV---SSSFVAMPGIPVCHSKDLINWQIIGHAYDSITfqpqyrMENEKTAYSRLcWAPTIRYDEgiyyigv 136
Cdd:cd18822    8 ILKVGGTYYWYgenRDNNNGFNGVSLYSSTDLVNWEFRNTVLTRDT------CSASELASCKI-ERPKVIYNP------- 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 137 niADDGFVMF-----------------KSTRPEGPYTMHK------FEKRlyDPGFFIDDDGKKYV---THGKGKIYLTR 190
Cdd:cd18822   74 --KTGKFVMWahwengkdyglaraavaTSDTPDGDYTFHGsfrplgYDSR--DMTLFVDDDGTAYLisaANDNADLNIYR 149

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 191 LKDDATGVldpqdkGTLIITapegyghLFEGCH-----TYKRNGWYYVFNP-ALGYDGVQ-MISRSRNLYGPYETKVLID 263
Cdd:cd18822  150 LTPDYLSV------DSLVAT-------LFKGQHreapaLVKRNGYYYLFTSgASGWYPNQgQYASATSLAGPWSSLRNIG 216

                        250       260
                 ....*....|....*....|....*....
gi 490450071 264 DDINYAGagvhQGGYI---ETAEGESWAY 289
Cdd:cd18822  217 NNTTFGS----QSTFIlpvGGSGGTSYLY 241
CBD_IV smart00606
Cellulose Binding Domain Type IV;
541-667 1.35e-07

Cellulose Binding Domain Type IV;


Pssm-ID: 128869 [Multi-domain]  Cd Length: 129  Bit Score: 50.79  E-value: 1.35e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071   541 HYDAFTEIDFFRYDDREGMTLVRPVGKRPMQFLTDIKDSDWLVFNNLTFKkRPGKITFELQ--ALNPGGVLEMRRGSLQG 618
Cdd:smart00606   1 GRDPYNAIQAESYDSQSGVQTETTSDAGGGKNVGYIDDGDWIAYKDVDFG-SSGAYTFTARvaSGNAGGSIELRLDSPTG 79

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|.
gi 490450071   619 ELLASCTIQSTNG--EWTKQSFEVKKLRKKEKVYFVFGGSNKsLSIKNFIF 667
Cdd:smart00606  80 TLVGTVDVPSTGGwqTYQTVSATVTLPAGVHDVYLVFKGGNY-FNIDWFRF 129
GH43-like cd08982
Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43) ...
 57-232 4.68e-07

Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350096 [Multi-domain]  Cd Length: 308  Bit Score: 52.18  E-value: 4.68e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMvsssFVAMPGiPVCHSKDLINWQIIghaydSITFQPQYrmenektaysrlCWAPTIR-YDEGIYYIG 135
Cdd:cd08982    7 DPTVVLFKGKYYL----FASKSG-GYWHSDDLVNWKFI-----PTNGLPIE------------DYAPTVVeINGTLYFTA 64

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 136 VNIADDgfvMFKSTRPEGPytmhKFEK-------RLYDPGFFIDDDGKKYVTHG---KGKIYLTRLkDDATGVLDPQDKG 205
Cdd:cd08982   65 SGGPGP---IYRTDDPLGG----KWELvaesgpfGFWDPALFVDDDGRLYLYWGcsnKDPIYGVEL-DPNTGFRPIGEPV 136

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 490450071 206 TLIITAPEGYG-------HLF-------EGCHTYKRNGWYY 232
Cdd:cd08982  137 PLISFDPDKHGwerfgedNEDpglapwiEGAWMTKHNGKYY 177
GH43_BT3675-like cd18828
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
 57-232 8.12e-07

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675); This glycosyl hydrolase family 43 (GH43) subgroup includes the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the GH43_bXyl subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl subgroup also includes enzymes annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350149 [Multi-domain]  Cd Length: 283  Bit Score: 51.12  E-value: 8.12e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMVSSS--FVAMPG--IPVCHSKDLINWQIIGHAYDSITFQPqyrmenekTAYSRLC-WAPTIRYDEGI 131
Cdd:cd18828    2 DPDIAYFDGKYYIYPTTdgFPGWSGtqFHVFSSDDLVTWKDEGVILDLKNDQV--------VPWATGNaWAPTIEERDGK 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 132 YY-------------IGVNIADDgfvmfkstrPEGPYTM-------HKFEK----RLYDPGFFIDD-DGKKYVTHGKGKI 186
Cdd:cd18828   74 YYfyfcgknpdgrsqIGVAVADS---------PTGPFTAqgsplitHEMARvtmgQAIDPSVFTDPvDGKYYLYWGNGYA 144

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 490450071 187 YLTRLKDDATGVldpqDKGTL-IITAPEGYghlFEGCHTYKRNGWYY 232
Cdd:cd18828  145 AIAELNDDMISI----KPGTLvNLDGLTDF---REAVTVLYRDGLYH 184
GH43_XynD-like cd09003
Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan ...
 85-232 3.66e-06

Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized Bacillus subtilis arabinoxylan arabinofuranohydrolase (AXH), Caldicellulosiruptor sp. Tok7B.1 beta-1,4-xylanase (EC 3.2.1.8) / alpha-L-arabinosidase (EC 3.2.1.55) XynA, Caldicellulosiruptor sp. Rt69B.1 xylanase C (EC 3.2.1.8) XynC, and Caldicellulosiruptor saccharolyticus beta-xylosidase (EC 3.2.1.37)/ alpha-L-arabinofuranosidase (EC 3.2.1.55) XynF. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. It belongs to the GH43_AXH-like subgroup which includes enzymes that have been annotated as having beta-xylosidase, alpha-L-arabinofuranosidase and arabinoxylan alpha-L-1,3-arabinofuranohydrolase, xylanase (endo-alpha-L-arabinanase) as well as AXH activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis AXH (BsAXH-m2,3) has been shown to cleave arabinose units from O-2- or O-3-mono-substituted xylose residues and superposition of its structure with known structures of the GH43 exo-acting enzymes, beta-xylosidase and alpha-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350117 [Multi-domain]  Cd Length: 315  Bit Score: 49.18  E-value: 3.66e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  85 SKDLINWQ---IIGHAydsitfqpqyrMENEKTAYSRLCWAPTIRYDEG-------IYY------IGVNIADDgfvmfks 148
Cdd:cd09003   54 SDDMVNWTdhgEIPVA-----------GPNGIAKWAGNSWAPSVAYKNIngkdkfyLYFanggggIGVLTADS------- 115

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 149 trPEGPYT-----------MHKFEKR--LYDPGFFIDDDGKKYVTHGKG------------KIYltRLKDDATGVldpqD 203
Cdd:cd09003  116 --PTGPWTdplgkplitrsTPGCAGVvwLFDPAVFIDDDGQGYLYFGGGvpggseanpktaRVI--KLGDDMISV----D 187

                        170       180
                 ....*....|....*....|....*....
gi 490450071 204 KGTLIITAPegygHLFEGCHTYKRNGWYY 232
Cdd:cd09003  188 GSAVTIDAP----YFFEASGINKINGKYY 212
GH43_CtGH43-like cd18824
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 57-257 5.00e-06

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350145 [Multi-domain]  Cd Length: 282  Bit Score: 48.56  E-value: 5.00e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGEDYYMVSSSFV---------AMPGIPVCHSKDLINWQIIGHAYDSITFQPQYRMenektaysrlCWAPTIRY 127
Cdd:cd18824    5 DGKIYFFGGAYYWYGTPYGcgcgscgftLFCGFVVYSSVDLVNWTYRGVLFDPNTCAGSPGV----------CFRPHVVY 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 D--EGIYYIGVNIADD--GFVMFKSTRPEGPYTMHKFEK------RLYDPGFFIDDDGKKYVTH----GKGKIYLTRLKD 193
Cdd:cd18824   75 NarTGRYVLWYNAYDGssGYAVATSSTPTGPFVTVPDPVlapaglQAGDFSLFVDDDGTGYLAYttidFPQSIVVEQLTD 154

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490450071 194 D-------ATGVLDPQDkgtliitapegyghlFEGCHTYKRNGWYYVF--NPALGYDGVQMIS--RSRNLYGPYE 257
Cdd:cd18824  155 DylnttgeYVRDLIDQE---------------AEAPSIFKRNGIYYILasNTCCGCCQGTGARvyRATSPLGPWT 214
GH43_Pc3Gal43A-like cd18821
Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1, ...
 60-234 6.62e-06

Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1,3-galactanase (Pc1, 3Gal43A, 1,3Gal43A); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), Fusarium oxysporum 12S Fo/1 (3Gal), and Streptomyces sp. 19(2012) SGalase1 and SGalase2. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350142 [Multi-domain]  Cd Length: 262  Bit Score: 48.00  E-value: 6.62e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  60 IIRVGEDYYMV-------SSSFvamPGIPVCHSKDLINWQIIGHAYDSIT---FQPQYRMENEKTAYSrlcwAPTIRY-- 127
Cdd:cd18821    8 ILKVGDTYYWFgedktdgSNLF---QGVSCYSSTDLVNWTFEGLALPPQEsgdLGPNRVVERPKVIYN----PSTGKYvm 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 ----DEGIY---YIGVNIADDgfvmfkstrPEGPYTMH---KFEKRLY-DPGFFIDDDGKKY-VTHGKGK-IYLTRLKDD 194
Cdd:cd18821   81 wmhiDSSNYgdaRVGVATSDT---------VTGPYTYVgsfRPLGYESrDIGVFQDDDGTAYlLFEDRDNgLRIYRLSDD 151

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 490450071 195 ATGVLdpqdkgTLIITAPEGYghlFEGCHTYKRNGWYYVF 234
Cdd:cd18821  152 YLSVV------ELVYTFIAAG---LEAPAMFKVDGTYYLL 182
CBM_6 pfam03422
Carbohydrate binding module (family 6);
573-668 8.77e-06

Carbohydrate binding module (family 6);


Pssm-ID: 397476  Cd Length: 125  Bit Score: 45.42  E-value: 8.77e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  573 LTDIKDSDWLVFNNLTFKKRpGKITFELQALN--PGGVLEMRRGSLQGELLASCTIQSTNG--EWTKQSFEVKKLRKKEK 648
Cdd:pfam03422  25 VGYIDNGDWIAYKDVDFGSG-GAYTFTARVASgaGGGSIELRLDSPTGTLIGTVSVPSTGGwqTYVTVSANVTLPTGVHD 103

                          90       100
                  ....*....|....*....|.
gi 490450071  649 VYFVFGGSNKSLS-IKNFIFE 668
Cdd:pfam03422 104 LYLVFTGGGGYLFnIDWFQFT 124
GH43_Bt3655-like cd08983
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
 47-234 1.40e-05

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 arabinofuranosidase Bt3655; This glycosyl hydrolase family 43 (GH43)-like family includes the characterized arabinofuranosidases (EC 3.2.1.55): Bacteroides thetaiotaomicron VPI-5482 (Bt3655;BT_3655) and Penicillium chrysogenum 31B Abf43B, as well as Bifidobacterium adolescentis ATCC 15703 beta-xylosidase (EC 3.2.1.37) BAD_1527. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350097  Cd Length: 262  Bit Score: 47.23  E-value: 1.40e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  47 TNPVINADYP-----DPDIIRVGED--YYMV--------SSSFVAMPGIPVCHSKDLINWqiighaydsiTFQPQYRMEN 111
Cdd:cd08983    5 GNPVLTSTVGtkgvrDPFIIRGPEDgkFYLVatdlwiagGAQWNGSRGIGVWESTDLVNW----------SEQRLVKMVS 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 112 EKTAysRLCWAPTIRYDE-----GIYY---IGVNIADDGFVMFKSTRPEgpytMHKF--EKRLYDPGF-FID----DDGK 176
Cdd:cd08983   75 PPNA--GNAWAPEAIYDPetgqyVVYWsssLYGDGGGGNHRIYYATTKD----FKTFsePKVLFDPGFnVIDttivKDGG 148

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 490450071 177 KYV------THGKGkIYLTRlkddATGVLDPQDKGTLIITAPEGYGhlFEGCHTYKRN--GWYYVF 234
Cdd:cd08983  149 TYYrfykdeTTGKG-IRLAT----SDSLTGPWTTVTTGGGAGTGGG--VEGPTVFKLNdgGKWYLY 207
GH43_CtGH43-like cd18825
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 60-259 2.01e-05

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350146 [Multi-domain]  Cd Length: 285  Bit Score: 46.82  E-value: 2.01e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  60 IIRVGEDYYMV-------SSSFVAMPGIPVCHSKDLINWQIIGHAYDSIT------FQPQYRME------NEKTA-YsrL 119
Cdd:cd18825    8 ILKHNGTYYWYgedktggTYRRVDVIGVSCYSSKDLYNWKDEGIVLDAVDdapasdLYPNNVVErpkviyNKKTKkY--V 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 120 CW----APTIRYDEGiyYIGVNIADdgfvmfkstRPEGPYT------MHKFEKRLYDPG--------FFIDDDGKKYVTH 181
Cdd:cd18825   86 MWfhldGPGADYSRA--RAGVAVSD---------SPTGPFKylgsfrPNAGEKNRDFSNgqmsrdmtLFVDDDGKAYLIY 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 182 ---GKGKIYLTRLKDDATGVLDPqdkgtliitapegYGHLFEGCHT-----YKRNGWYYVF---------NPALGYdgvq 244
Cdd:cd18825  155 sseENKTLYIAKLTDDYTGVTGD-------------YARILIGQSReapavFKHDGKYYMItsgctgwapNAARYA---- 217

                        250
                 ....*....|....*
gi 490450071 245 misRSRNLYGPYETK 259
Cdd:cd18825  218 ---VADSIFGPWKEI 229
GH43_CtGH43-like cd08985
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
 60-316 4.04e-05

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350099 [Multi-domain]  Cd Length: 273  Bit Score: 45.78  E-value: 4.04e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  60 IIRVGEDYYMVSSSFVAMP------GIPVCHSKDLINWQIIG---HAYDSITFQ---PQYRMENEKTAYSrlcwAPTIRY 127
Cdd:cd08985    8 ILQEGDTYYWYGESRKGLDndnlshGINCYSSTDLYNWRFEGlvlPASGVEVVRdisPGYVIERPKVLYN----ARTRKY 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 128 degIYYIGVNIADDGFVM---FKSTRPEGPYTMHKFEKRL----YDPGFFIDDDGKKYV---THGKGKIYLTRLKDDATG 197
Cdd:cd08985   84 ---VMWFHLDNPNYGFAAvgvATSDTPTGPFTFVRSFRPDgypsRDMTLFQDPDGTAYLvrsTDHNTDIGISRLSDDYLD 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 198 VLDPQDKGTliitapegyGHLFEGCHTYKRNGWYYVF-------NPALGYdgvqmISRSRNLYGPYETKVLIDDDINYAG 270
Cdd:cd08985  161 TTGASSTFK---------GPKREAPALFKRGGTYYLItsgltgwNPNPSR-----LARADSPLGPWSTWGNLPVGGPGAD 226

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*.
gi 490450071 271 agvhqggyieTAEGESWAYTFQDRDYMGRCLMLYPMKWENEWPVVG 316
Cdd:cd08985  227 ----------TTYDSQPAFVFPVEGQGGELFIYMGDRWNPGGGGVG 262
GH43_AnAbnA-like cd18831
Glycosyl hydrolase family 43 protein such as Aspergillus niger endo-alpha-L-arabinanase (AbnA); ...
 166-256 5.53e-05

Glycosyl hydrolase family 43 protein such as Aspergillus niger endo-alpha-L-arabinanase (AbnA); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities such as Aspergillus niger AbnA, Aspergillus niveus AbnA, and Chrysosporium lucknowense Abn1. It belongs to the GH43_Arb43a subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_Arb43a subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. The GH43_Arb43a subgroup includes many enzymes such as Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, and are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350152 [Multi-domain]  Cd Length: 286  Bit Score: 45.66  E-value: 5.53e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 166 DPGFFIDDDGKKYVTHGK--GKIYLTRLKDDATGVLDPQDkGTLIITAPEGyGHLFEGCHTYKRNGWYYVF--------- 234
Cdd:cd18831  116 DPNLIVDDDGTPYLTFGSywQGIFQVPLTDPLLSPAAGPP-PTHLAYNPSG-NHPEEGSFMYKHGGYYYLFfssgiccgy 193

                         90       100
                 ....*....|....*....|....*
gi 490450071 235 NPALGYDGVQ---MISRSRNLYGPY 256
Cdd:cd18831  194 DPSLPAPGEEykiRVCRSTSPTGPF 218
GH43_CtGH43-like cd18826
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 85-257 9.47e-05

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350147 [Multi-domain]  Cd Length: 269  Bit Score: 44.54  E-value: 9.47e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  85 SKDLINWQ----IIGHAYDSIT--FQPQYRME------NEKT-AYsrLCWApTIRYDEGIYYIGVNIADdgfvmfkstRP 151
Cdd:cd18826   41 STDLYNWEdeglIIPPDPDDPSspLHPTRIMDrphiiyNEKTgKY--VCWL-KLYPGGDVQYFGVLTAD---------SP 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 152 EGPYTMHKfeKRLYDPGF-------FIDDDGKKYVTHGK--GKIYLTRLKDDATGVLDP---QDKGTLIITAPEGYGHlf 219
Cdd:cd18826  109 TGPYTYVH--KFLGPLGMgagdfdlVVDPDGKAYLYFERvhKEVVCADLTDDYTDVTGEystHFPGLGPPFAREAPAV-- 184

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 490450071 220 egchtYKRNGWYYVF---------NPALgydgvqmISRSRNLYGPYE 257
Cdd:cd18826  185 -----FKRGGKHYLLtsgttgyfpNPSE-------VAVADSYHGPWT 219
GH43_LbAraf43-like cd18820
Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis ...
 56-234 1.75e-04

Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350141 [Multi-domain]  Cd Length: 258  Bit Score: 43.67  E-value: 1.75e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  56 PDPDIIRVGEDYYMVSSSfvaMPGIPVCHSKDLINWqiiGHAYDSITFQPqyrmenEKTAYSRLCWAPTIRYDEGIYYIG 135
Cdd:cd18820    1 ADPWVVYHDGYYYLTFTT---GDRITIWKSKTLTGL---GTAEPKVVWTP------PDPSRSCNIWAPELHFIDGRWYIY 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 136 VNiADDG-------FVMfKSTR---PEGPYTmhkFEKRLYDPGFF--ID-----DDGKKY--------VTHGKGKIYLTR 190
Cdd:cd18820   69 YA-ADDGdnanhrmYVL-ESASddpPLGPYT---FKGRLADPTDKwaIDgtvleHNGKLYfvwsgwegDTNGPQNLYIAP 143

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 490450071 191 LKDDATGVLDPQdkgtlIITAP----EGYGHLF--EGCHTYKRNGWYYVF 234
Cdd:cd18820  144 MSNPWTLSGERV-----LISTPtydwERQGPPGvnEGPQVLYHGGRTFLV 188
GH43_Bt1873-like cd08981
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron BT_1873; This ...
 57-194 1.30e-03

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron BT_1873; This glycosyl hydrolase family 43 (GH43) subfamily includes Bacteroides thetaiotaomicron VPI-5482 endo-arabinase (Bt1873;BT_1873), as well as uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the GH43 enzymes in this family may display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350095 [Multi-domain]  Cd Length: 289  Bit Score: 41.36  E-value: 1.30e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071  57 DPDIIRVGED--YYMVSSSFV-----AMPGIPVCHSKDLINWQiiGhaydsitfqPQYRMENEKTAYSRLC-WAPTIRYD 128
Cdd:cd08981    8 DPFILADKKTgtYYLYGTTDKdcwwgKGTGFDVYVSKDLENWE--G---------PYEVFRPPEDFWADRNfWAPEVHEY 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490450071 129 EGIYYIGVNIADDGFVM-----FKSTRPEGPYTMHKfEKRLYDPG-------FFIDDDGKKYV--TH-----GKGKIYLT 189
Cdd:cd08981   77 NGKYYLFATFKAEGNGRrgtqiLVSDSPLGPFVPLS-DGPVTPEDwmcldgtLYVDEDGKPWMvfCHewvqvGDGTICAV 155


                 ....*
gi 490450071 190 RLKDD 194
Cdd:cd08981  156 RLSDD 160
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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