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Conserved domains on  [gi|489998612|ref|WP_003901617|]
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MULTISPECIES: tRNA (cytidine(34)-2'-O)-methyltransferase [Mycobacterium]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-154 1.19e-89

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 257.65  E-value: 1.19e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  81 QATTLFTNVGYHAGDVLMFGPEPTGLDEATLADtHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-154 1.19e-89

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 257.65  E-value: 1.19e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  81 QATTLFTNVGYHAGDVLMFGPEPTGLDEATLADtHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-147 2.35e-72

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 213.47  E-value: 2.35e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   3 RLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTAQA 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489998612  83 TTLFTNVGYHAGDVLMFGPEPTGLDEAtLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQ 147
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEE-ILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQ 144
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 1.42e-58

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 179.15  E-value: 1.42e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612    1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612   81 QATTLFTNVGYHAGDVLMFGPEPTGLdEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGL-PQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGLV 153
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-154 3.24e-56

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 173.26  E-value: 3.24e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  81 QATTLFTNVGYHAGDVLMFGPEPTGLDEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 8.99e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 120.71  E-value: 8.99e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612    1 MFRLLFVSPRIAPNTGNAIRTCAATGC-ELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPA--RVFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489998612   78 FTAQATTLFtNVGYHAGDVLMFGPEPTGLDEATLADTHITgqVRIPMLAGRRSLNLSNAAAVAVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLELCDRL--VRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-154 1.19e-89

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 257.65  E-value: 1.19e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  81 QATTLFTNVGYHAGDVLMFGPEPTGLDEATLADtHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGLV 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 3-147 2.35e-72

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 213.47  E-value: 2.35e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   3 RLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTAQA 82
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489998612  83 TTLFTNVGYHAGDVLMFGPEPTGLDEAtLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQ 147
Cdd:cd18094   81 TKPYTDFDFKPGDYLVFGRESTGLPEE-ILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQ 144
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-154 1.42e-58

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 179.15  E-value: 1.42e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612    1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612   81 QATTLFTNVGYHAGDVLMFGPEPTGLdEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGL-PQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGLV 153
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-154 3.24e-56

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 173.26  E-value: 3.24e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   1 MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  81 QATTLFTNVGYHAGDVLMFGPEPTGLDEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV 154
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 8.99e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 120.71  E-value: 8.99e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612    1 MFRLLFVSPRIAPNTGNAIRTCAATGC-ELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPA--RVFA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGAdGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489998612   78 FTAQATTLFtNVGYHAGDVLMFGPEPTGLDEATLADTHITgqVRIPMLAGRRSLNLSNAAAVAVY 142
Cdd:pfam00588  81 TSLGGKSLY-EIDFPKKVALVFGNEGTGLSNEVLELCDRL--VRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 3-144 1.22e-18

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 76.88  E-value: 1.22e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   3 RLLFVSPRIAPNTGNAIRTCAATGCE-LHLVEPLGFDLSePKLRRAGLDYHDLASVTVHASLAHAWEALSPA--RVFAFT 79
Cdd:cd18082    1 HLVLDNIRSPGNIGSIFRTADAFGVEkIYLVGPTADIFD-PKAKRTSRGTHFSVPYERVDNFEEYLKSLKEEgyPLIGLE 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489998612  80 AQATTL---FTNVGYHAGDVLMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18082   80 LTDGSIplsEFKFKKPKKIALIFGNERSGLSPELLA--LCDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 2-143 2.54e-14

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 65.98  E-value: 2.54e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   2 FRLLFVSPRIAPNTGNAIRTCAATGC-ELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAhawEALSPA-RVFAFT 79
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLsDLRLVNPRCDDPEEAARALASGAEDVLENAKVYDTLE---EALADCdLVVATT 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489998612  80 AQATTLFTNV------------GYHAGDV-LMFGPEPTGL--DEATLADTHITgqvrIPMLAGRRSLNLSNAAAVAVYE 143
Cdd:cd18093   78 ARPRGLRRPLltprelaellkeHAAGGKVaLVFGREDSGLtnEELALCDLLVT----IPTNPEYPSLNLAQAVAIVLYE 152
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 14-147 4.68e-14

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 64.91  E-value: 4.68e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLD---YHDLASVTvhASLAHAWEALSPARVFAFTAQATTLFTNVG 90
Cdd:cd18104   13 NLGAILRSADAAGADAVILCDPKTDLFNPNVIRASLGavfTLPVAEAS--SEETLAWLKENKIRILATTPKAEKLYYEAD 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 489998612  91 YHAGDVLMFGPEPTGLDEATLADTHItgQVRIPMLAGRRSLNLSNAAAVAVYEAWRQ 147
Cdd:cd18104   91 LRGPIAIVVGSESEGLSEFWLKAADE--RVRIPMAGQADSLNVSVAAAILLYEALRQ 145
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 14-151 1.03e-11

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 60.59  E-value: 1.03e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPA--RVFAFTAQATTLFTNVGY 91
Cdd:COG0566  128 NLGAILRSAAAFGVDGVILPKRRADPYNPKVVRASAGALFHVPVARVTNLADALEELKEAgfWVVGTDLDGEASLYEVDL 207

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  92 HAGDVLMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFA 151
Cdd:COG0566  208 PGPVALVLGNEGKGLSRLVLE--ACDELVRIPMAGGVESLNVAVAAAILLYEAARQRRAK 265
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 3-153 5.07e-10

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 55.89  E-value: 5.07e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   3 RLLFVSPRIAPNTGNAIRTCAATG-CELHLVEPLGFdLSEPKLRRA--GLDYhdLASVTVHASLAhawEALSPA-RVFAF 78
Cdd:COG0565    5 RVVLVEPQHPGNIGAAARAMKNFGlSDLRLVNPRGW-PDEEARAMAsgADDV--LENARVVDTLE---EALADCdLVVGT 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  79 TA-------------QATTLFTNVGYHaGDV-LMFGPEPTGL--DEATLADTHITgqvrIPMLAGRRSLNLSNAAAVAVY 142
Cdd:COG0565   79 TArprelrkpvltprEAAEELLARAAG-GKVaLVFGRERSGLtnEELALCDALVT----IPTNPEYPSLNLAQAVQVVAY 153

                        170
                 ....*....|.
gi 489998612 143 EaWRQHGFAGA 153
Cdd:COG0565  154 E-LRKAALEAP 163
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 10-144 1.78e-09

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 52.95  E-value: 1.78e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  10 RIAPNTGNAIRTCAATGCE-LHLVeplGFDLSEP--KLRRAGLDYHDLASVTVHASLAHAWEALSPA--RVFAF-TAQAT 83
Cdd:cd18097    9 RSAHNVGSIFRTADAAGVEkIYLC---GYTPTPPhpKVAKTALGAEESVPWEHFEDTLEAVRALKARgyTIIALeTTENS 85

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489998612  84 TLFTNVGYHAGDVLMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18097   86 VPLHEARLPRPLALVLGNEVRGVSPEVLA--LCDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 19-152 1.93e-09

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 54.23  E-value: 1.93e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  19 IRTCAATGC-ELHLVEPlgfdlsEPKLRRAGldyhDLAS-------VTVHASLAHAwealsparVFAFTAQ-----ATTL 85
Cdd:PRK11081  37 IRTADAVGVhEVHAVWP------GSRMRTMG----STAAgsnswvqVKTHRTIGDA--------VAHLKGQgmqilATHL 98

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489998612  86 ------FTNVGYHAGDVLMFGPEPTGL-DEA-TLADTHITgqvrIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAG 152
Cdd:PRK11081  99 sdtavdFREIDYTRPTCILMGQEKTGIsQEAlALADQDII----IPMIGMVQSLNVSVASALILYEAQRQRQNAG 169
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 14-144 7.90e-08

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 48.40  E-value: 7.90e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHdlasVTVHASLAHAWEALS---PARVFAFTAQA---TTLFT 87
Cdd:cd18106   13 NLGSILRSAAAAGCSKVLLTKGCVDPWEPKVLRAGAGAH----FRLPIYGNLSWDDIPnylPDDSKVFLADNnlpVQPYD 88

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  88 NVGYHAGD-VLMFGPEPTGLDEATLADTHITG--QVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18106   89 SIDYCSGPiVLVIGGETHGVSPEAYKLAHSTGgsRVHIPLANGVDSLNSAVAASILLFEM 148
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 14-144 6.19e-07

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 45.84  E-value: 6.19e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRA---GLDYHDLASVTvhaSLAHAWEALSPA--RVFAFTAQATTLFTN 88
Cdd:cd18103   13 NLGAIIRSAEAFGVDGVIIPKRRSAPLTPTVAKAsagALEHLPIARVT---NLARALEELKEAgfWIYGADGEAEKSLYD 89

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 489998612  89 VGYHAGDVLMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18103   90 VDLTGPLALVLGSEGKGLRRLVLK--NCDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 14-144 1.69e-06

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 44.72  E-value: 1.69e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRA---GLDYHDLASVTVHASLAHAWEAlSPARVFAFTAQATTLFTNVG 90
Cdd:cd18095   13 NLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRAsmgALFRLPIIRVDDLEELLEELKE-AGYKVVAADLDGGSSLYDLD 91

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 489998612  91 YHAGDVLMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18095   92 LPKPVALVLGNEGNGLSDELLE--LCDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 14-147 2.89e-06

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 44.38  E-value: 2.89e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  14 NTGNA---IRTCAATGC-ELHLVEPlgfDLSEPKLRRAGLDYHDLASVTVHASLAhawEALSPARVFAFTAQATTLFTN- 88
Cdd:cd18092   26 DPHNAsavLRTCDAFGVqDVHVIEP---KNKFKKNRGVAMGADKWLDVHRHDSTE---ECLAALKEQGYRIVATSLSPDa 99

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489998612  89 VGYHAGDV-----LMFGPEPTGLDEATLAdtHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQ 147
Cdd:cd18092  100 VPLDELDYtrptaLVFGNEKDGVSEEALA--AADERVYIPMYGFVESLNVSVAAAIILYELQRQ 161
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 4-144 1.55e-04

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 39.68  E-value: 1.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612   4 LLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRA--GLDYHDLAS-VTVHASLaHAWEALSPARVFAFTA 80
Cdd:cd18107    3 VLFDRPTSPGNLGTIIRSADALGAHGLVVTGHAADVYDPKTIRAstGSLFSLPIVrVPSNREL-EDWVEARKARGTPLQL 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489998612  81 QATTLFTNVGYHAGD-----VLMFGPEPTGLDEA--TLADthitGQVRIPMLAGRRSLNLSNAAAVAVYEA 144
Cdd:cd18107   82 VGTDEHGDHQIADHDftrptLLLIGNETAGLSAAwrELCD----ETVSIPMAGSASSLNAACAASIFLYEA 148
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 97-143 1.20e-03

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 37.76  E-value: 1.20e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 489998612   97 LMFGPEPTGL--DEATLADTHitgqVRIPMLAGRRSLNLSNAAAVAVYE 143
Cdd:TIGR00050 108 LVFGREDSGLtnEELQKCHVL----VSIPTSEEYPSLNLSHAVAVILYE 152
PRK10864 PRK10864
putative methyltransferase; Provisional
 15-148 1.56e-03

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 37.45  E-value: 1.56e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489998612  15 TGNAIRTcAATGCElHlVEPL-GFDLSE--PKLRRAGldYhdlasvTVHASLAHAWEALS----PARVfaftaqattlft 87
Cdd:PRK10864 237 SGAAIRT-AEGGAE-H-VQPItGDSFVDvlDDFRQAG--Y------TIVTTSSHKGTPLFkaslPAKM------------ 293

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 489998612  88 nvgyhagdVLMFGPEPTGLDEATLADTHITgqVRIPMLAGRRSLNLSNAAAVAVYEAWRQH 148
Cdd:PRK10864 294 --------VLVLGQEYDGLSDAARQQGDLS--VSIDGTGNVESLNVSVATGVLLAEWWRQN 344
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 96-143 9.67e-03

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 34.43  E-value: 9.67e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 489998612  96 VLMFGPEPTGLDEATLADTHITgqVRIPMLAGRRSLNLSNAAAVAVYE 143
Cdd:cd18091   99 VLVLGNEKEGIPAELLSLLDVC--VEIPQLGVIRSLNVHVSGAIAIYE 144
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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