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Conserved domains on  [gi|489505128|ref|WP_003410009|]
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MULTISPECIES: dehydrogenase [Mycobacterium tuberculosis complex]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NADB_Rossmann super family cl21454
Rossmann-fold NAD(P)(+)-binding proteins; A large family of proteins that share a ...
 4-27 1.90e-11

Rossmann-fold NAD(P)(+)-binding proteins; A large family of proteins that share a Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The NADB domain is found in numerous dehydrogenases of metabolic pathways such as glycolysis, and many other redox enzymes. NAD binding involves numerous hydrogen-bonds and van der Waals contacts, in particular H-bonding of residues in a turn between the first strand and the subsequent helix of the Rossmann-fold topology. Characteristically, this turn exhibits a consensus binding pattern similar to GXGXXG, in which the first 2 glycines participate in NAD(P)-binding, and the third facilitates close packing of the helix to the beta-strand. Typically, proteins in this family contain a second domain in addition to the NADB domain, which is responsible for specifically binding a substrate and catalyzing a particular enzymatic reaction.


The actual alignment was detected with superfamily member TIGR03971:

Pssm-ID: 473865 [Multi-domain]  Cd Length: 270  Bit Score: 58.64  E-value: 1.90e-11
                          10        20
                  ....*....|....*....|....
gi 489505128    4 LEGKVAFITGVARGQGRSHAVRLA 27
Cdd:TIGR03971   1 LEGKVAFITGAARGQGRSHAVRLA 24
fabG super family cl36191
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 1-73 2.18e-07

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


The actual alignment was detected with superfamily member PRK12825:

Pssm-ID: 237218 [Multi-domain]  Cd Length: 249  Bit Score: 47.17  E-value: 2.18e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARAL--GKVDVEACGALVGEVEVWGR-------DVRD--DRRVFVESPAD 69
Cdd:PRK12825   1 MGSLMGRVALVTGAARGLGRAIALRLARAGADVVvhYRSDEEAAEELVEAVEALGRraqavqaDVTDkaALEAAVAAAVE 80


                 ....
gi 489505128  70 EFGA 73
Cdd:PRK12825  81 RFGR 84
 
Name Accession Description Interval E-value
SDR_subfam_1 TIGR03971
SDR family mycofactocin-dependent oxidoreductase; Members of this protein subfamily are ...
 4-27 1.90e-11

SDR family mycofactocin-dependent oxidoreductase; Members of this protein subfamily are putative oxidoreductases belonging to the larger SDR family. All members occur in genomes that encode a cassette for the biosynthesis of mycofactocin, a proposed electron carrier of a novel redox pool. Characterized members of this family are described as NDMA-dependent, meaning that a blue aniline dye serving as an artificial electron acceptor is required for members of this family to cycle in vitro, since the bound NAD residue is not exchangeable. See EC 1.1.99.36. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 274889 [Multi-domain]  Cd Length: 270  Bit Score: 58.64  E-value: 1.90e-11
                          10        20
                  ....*....|....*....|....
gi 489505128    4 LEGKVAFITGVARGQGRSHAVRLA 27
Cdd:TIGR03971   1 LEGKVAFITGAARGQGRSHAVRLA 24
fabG PRK12825
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 1-73 2.18e-07

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


Pssm-ID: 237218 [Multi-domain]  Cd Length: 249  Bit Score: 47.17  E-value: 2.18e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARAL--GKVDVEACGALVGEVEVWGR-------DVRD--DRRVFVESPAD 69
Cdd:PRK12825   1 MGSLMGRVALVTGAARGLGRAIALRLARAGADVVvhYRSDEEAAEELVEAVEALGRraqavqaDVTDkaALEAAVAAAVE 80


                 ....
gi 489505128  70 EFGA 73
Cdd:PRK12825  81 RFGR 84
FabG COG1028
NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family [Lipid transport and ...
 1-72 6.17e-06

NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family [Lipid transport and metabolism]; NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440651 [Multi-domain]  Cd Length: 249  Bit Score: 42.85  E-value: 6.17e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGRDVR---------DDRRVFVESPADE 70
Cdd:COG1028    1 MTRLKGKVALVTGGSSGIGRAIARALAAEGARvVITDRDAEALEAAAAELRAAGGRALavaadvtdeAAVEALVAAAVAA 80


                 ..
gi 489505128  71 FG 72
Cdd:COG1028   81 FG 82
PRK12826 PRK12826
SDR family oxidoreductase;
1-32 1.09e-05

SDR family oxidoreductase;


Pssm-ID: 183775 [Multi-domain]  Cd Length: 251  Bit Score: 42.21  E-value: 1.09e-05
                         10        20        30
                 ....*....|....*....|....*....|..
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR 32
Cdd:PRK12826   1 TRDLEGRVALVTGAARGIGRAIAVRLAADGAE 32
3beta-17beta-HSD_like_SDR_c cd05341
3beta17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs; This subgroup includes ...
 2-69 4.87e-04

3beta17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs; This subgroup includes members identified as 3beta17beta hydroxysteroid dehydrogenase, 20beta hydroxysteroid dehydrogenase, and R-alcohol dehydrogenase. These proteins exhibit the canonical active site tetrad and glycine rich NAD(P)-binding motif of the classical SDRs. 17beta-dehydrogenases are a group of isozymes that catalyze activation and inactivation of estrogen and androgens, and include members of the SDR family. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H)-binding pattern (typically, TGxxxGxG in classical SDRs and TGxxGxxG in extended SDRs), while substrate binding is in the C-terminal region. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr and Lys, as well as Asn (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.


Pssm-ID: 187600 [Multi-domain]  Cd Length: 247  Bit Score: 37.75  E-value: 4.87e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489505128   2 GRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGRDVRDDrrvfVESPAD 69
Cdd:cd05341    1 NRLKGKVAIVTGGARGLGLAHARLLVAEGAKvVLSDILDEEGQAAAAELGDAARFFHLD----VTDEDG 65
 
Name Accession Description Interval E-value
SDR_subfam_1 TIGR03971
SDR family mycofactocin-dependent oxidoreductase; Members of this protein subfamily are ...
 4-27 1.90e-11

SDR family mycofactocin-dependent oxidoreductase; Members of this protein subfamily are putative oxidoreductases belonging to the larger SDR family. All members occur in genomes that encode a cassette for the biosynthesis of mycofactocin, a proposed electron carrier of a novel redox pool. Characterized members of this family are described as NDMA-dependent, meaning that a blue aniline dye serving as an artificial electron acceptor is required for members of this family to cycle in vitro, since the bound NAD residue is not exchangeable. See EC 1.1.99.36. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 274889 [Multi-domain]  Cd Length: 270  Bit Score: 58.64  E-value: 1.90e-11
                          10        20
                  ....*....|....*....|....
gi 489505128    4 LEGKVAFITGVARGQGRSHAVRLA 27
Cdd:TIGR03971   1 LEGKVAFITGAARGQGRSHAVRLA 24
fabG PRK12825
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 1-73 2.18e-07

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


Pssm-ID: 237218 [Multi-domain]  Cd Length: 249  Bit Score: 47.17  E-value: 2.18e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARAL--GKVDVEACGALVGEVEVWGR-------DVRD--DRRVFVESPAD 69
Cdd:PRK12825   1 MGSLMGRVALVTGAARGLGRAIALRLARAGADVVvhYRSDEEAAEELVEAVEALGRraqavqaDVTDkaALEAAVAAAVE 80


                 ....
gi 489505128  70 EFGA 73
Cdd:PRK12825  81 RFGR 84
PRK08589 PRK08589
SDR family oxidoreductase;
1-72 4.39e-07

SDR family oxidoreductase;


Pssm-ID: 181491 [Multi-domain]  Cd Length: 272  Bit Score: 46.31  E-value: 4.39e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARALG-------KVDVEACGALVGEVEVWGRDVRDDRRV--FVESPADEF 71
Cdd:PRK08589   1 MKRLENKVAVITGASTGIGQASAIALAQEGAYVLAvdiaeavSETVDKIKSNGGKAKAYHVDISDEQQVkdFASEIKEQF 80


                 .
gi 489505128  72 G 72
Cdd:PRK08589  81 G 81
PRK12828 PRK12828
short chain dehydrogenase; Provisional
 1-72 5.67e-06

short chain dehydrogenase; Provisional


Pssm-ID: 237220 [Multi-domain]  Cd Length: 239  Bit Score: 43.25  E-value: 5.67e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARAL----GKVDVEAC--GALVGEVEVWGRDVRD--DRRVFVESPADEFG 72
Cdd:PRK12828   2 EHSLQGKVVAITGGFGGLGRATAAWLAARGARVAligrGAAPLSQTlpGVPADALRIGGIDLVDpqAARRAVDEVNRQFG 81
FabG COG1028
NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family [Lipid transport and ...
 1-72 6.17e-06

NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family [Lipid transport and metabolism]; NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440651 [Multi-domain]  Cd Length: 249  Bit Score: 42.85  E-value: 6.17e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGRDVR---------DDRRVFVESPADE 70
Cdd:COG1028    1 MTRLKGKVALVTGGSSGIGRAIARALAAEGARvVITDRDAEALEAAAAELRAAGGRALavaadvtdeAAVEALVAAAVAA 80


                 ..
gi 489505128  71 FG 72
Cdd:COG1028   81 FG 82
PRK12826 PRK12826
SDR family oxidoreductase;
1-32 1.09e-05

SDR family oxidoreductase;


Pssm-ID: 183775 [Multi-domain]  Cd Length: 251  Bit Score: 42.21  E-value: 1.09e-05
                         10        20        30
                 ....*....|....*....|....*....|..
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR 32
Cdd:PRK12826   1 TRDLEGRVALVTGAARGIGRAIAVRLAADGAE 32
PRK07063 PRK07063
SDR family oxidoreductase;
1-86 7.64e-05

SDR family oxidoreductase;


Pssm-ID: 235924 [Multi-domain]  Cd Length: 260  Bit Score: 40.03  E-value: 7.64e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEvevWGRDVRDDRRVFVESPADEFGACRRVAR 79
Cdd:PRK07063   2 MNRLAGKVALVTGAAQGIGAAIARAFAREGAAvALADLDAALAERAAAA---IARDVAGARVLAVPADVTDAASVAAAVA 78


                 ....*..
gi 489505128  80 QGIRVVG 86
Cdd:PRK07063  79 AAEEAFG 85
PRK07067 PRK07067
L-iditol 2-dehydrogenase;
1-72 1.36e-04

L-iditol 2-dehydrogenase;


Pssm-ID: 235925 [Multi-domain]  Cd Length: 257  Bit Score: 39.24  E-value: 1.36e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEV--EVWG--RDV--RDDRRVFVESPADEFG 72
Cdd:PRK07067   1 MMRLQGKVALLTGAASGIGEAVAERYLAEGARvVIADIKPARARLAALEIgpAAIAvsLDVtrQDSIDRIVAAAVERFG 79
PRK08278 PRK08278
SDR family oxidoreductase;
1-72 1.91e-04

SDR family oxidoreductase;


Pssm-ID: 181349 [Multi-domain]  Cd Length: 273  Bit Score: 38.73  E-value: 1.91e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLA-DGQARAL-GKVD----------------VEACG----ALVGevevwgrDVRD 58
Cdd:PRK08278   1 MMSLSGKTLFITGASRGIGLAIALRAArDGANIVIaAKTAephpklpgtihtaaeeIEAAGgqalPLVG-------DVRD 73

                         90
                 ....*....|....*.
gi 489505128  59 DRRV--FVESPADEFG 72
Cdd:PRK08278  74 EDQVaaAVAKAVERFG 89
PRK12827 PRK12827
short chain dehydrogenase; Provisional
 1-27 2.13e-04

short chain dehydrogenase; Provisional


Pssm-ID: 237219 [Multi-domain]  Cd Length: 249  Bit Score: 38.55  E-value: 2.13e-04
                         10        20
                 ....*....|....*....|....*..
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLA 27
Cdd:PRK12827   1 MASLDSRRVLITGGSGGLGRAIAVRLA 27
PRK07326 PRK07326
SDR family oxidoreductase;
1-72 2.99e-04

SDR family oxidoreductase;


Pssm-ID: 235990 [Multi-domain]  Cd Length: 237  Bit Score: 38.07  E-value: 2.99e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLA-DGQARALGKVDVEACGALVGEVEVWGR------DVRD--DRRVFVESPADEF 71
Cdd:PRK07326   1 MMSLKGKVALITGGSKGIGFAIAEALLaEGYKVAITARDQKELEEAAAELNNKGNvlglaaDVRDeaDVQRAVDAIVAAF 80


                 .
gi 489505128  72 G 72
Cdd:PRK07326  81 G 81
PRK06198 PRK06198
short chain dehydrogenase; Provisional
 1-86 2.99e-04

short chain dehydrogenase; Provisional


Pssm-ID: 180462 [Multi-domain]  Cd Length: 260  Bit Score: 38.45  E-value: 2.99e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARALGKV--DVEACGALVGEVEVWGRDVrddrrVFVESPADEFGACRRVA 78
Cdd:PRK06198   1 MGRLDGKVALVTGGTQGLGAAIARAFAERGAAGLVICgrNAEKGEAQAAELEALGAKA-----VFVQADLSDVEDCRRVV 75


                 ....*...
gi 489505128  79 RQGIRVVG 86
Cdd:PRK06198  76 AAADEAFG 83
FabG-like PRK07231
SDR family oxidoreductase;
3-72 3.12e-04

SDR family oxidoreductase;


Pssm-ID: 235975 [Multi-domain]  Cd Length: 251  Bit Score: 38.27  E-value: 3.12e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489505128   3 RLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGR------DV--RDDRRVFVESPADEFG 72
Cdd:PRK07231   2 RLEGKVAIVTGASSGIGEGIARRFAAEGARvVVTDRNEEAAERVAAEILAGGRaiavaaDVsdEADVEAAVAAALERFG 80
fabG PRK05653
3-oxoacyl-ACP reductase FabG;
3-57 4.74e-04

3-oxoacyl-ACP reductase FabG;


Pssm-ID: 235546 [Multi-domain]  Cd Length: 246  Bit Score: 37.83  E-value: 4.74e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 489505128   3 RLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGRDVR 57
Cdd:PRK05653   2 SLQGKTALVTGASRGIGRAIALRLAADGAKvVIYDSNEEAAEALAAELRAAGGEAR 57
3beta-17beta-HSD_like_SDR_c cd05341
3beta17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs; This subgroup includes ...
 2-69 4.87e-04

3beta17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs; This subgroup includes members identified as 3beta17beta hydroxysteroid dehydrogenase, 20beta hydroxysteroid dehydrogenase, and R-alcohol dehydrogenase. These proteins exhibit the canonical active site tetrad and glycine rich NAD(P)-binding motif of the classical SDRs. 17beta-dehydrogenases are a group of isozymes that catalyze activation and inactivation of estrogen and androgens, and include members of the SDR family. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H)-binding pattern (typically, TGxxxGxG in classical SDRs and TGxxGxxG in extended SDRs), while substrate binding is in the C-terminal region. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr and Lys, as well as Asn (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.


Pssm-ID: 187600 [Multi-domain]  Cd Length: 247  Bit Score: 37.75  E-value: 4.87e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489505128   2 GRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVEVWGRDVRDDrrvfVESPAD 69
Cdd:cd05341    1 NRLKGKVAIVTGGARGLGLAHARLLVAEGAKvVLSDILDEEGQAAAAELGDAARFFHLD----VTDEDG 65
fabG PRK05565
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 2-27 6.69e-04

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


Pssm-ID: 235506 [Multi-domain]  Cd Length: 247  Bit Score: 37.13  E-value: 6.69e-04
                         10        20
                 ....*....|....*....|....*.
gi 489505128   2 GRLEGKVAFITGVARGQGRSHAVRLA 27
Cdd:PRK05565   1 MKLMGKVAIVTGASGGIGRAIAELLA 26
fabG PRK08217
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 3-73 1.30e-03

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


Pssm-ID: 181297 [Multi-domain]  Cd Length: 253  Bit Score: 36.48  E-value: 1.30e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   3 RLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVD-------VEACGALVGEVEVWGRDVRDDRRVF--VESPADEFG 72
Cdd:PRK08217   2 DLKDKVIVITGGAQGLGRAMAEYLAQKGAKlALIDLNqekleeaVAECGALGTEVRGYAANVTDEEDVEatFAQIAEDFG 81


                 .
gi 489505128  73 A 73
Cdd:PRK08217  82 Q 82
SDH_SDR_c cd05363
Sorbitol dehydrogenase (SDH), classical (c) SDR; This bacterial subgroup includes Rhodobacter ...
 4-50 1.79e-03

Sorbitol dehydrogenase (SDH), classical (c) SDR; This bacterial subgroup includes Rhodobacter sphaeroides SDH, and other SDHs. SDH preferentially interconverts D-sorbitol (D-glucitol) and D-fructose, but also interconverts L-iditol/L-sorbose and galactitol/D-tagatose. SDH is NAD-dependent and is a dimeric member of the SDR family. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H)-binding pattern (typically, TGxxxGxG in classical SDRs and TGxxGxxG in extended SDRs), while substrate binding is in the C-terminal region. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr and Lys, as well as Asn (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.


Pssm-ID: 187621 [Multi-domain]  Cd Length: 254  Bit Score: 36.06  E-value: 1.79e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 489505128   4 LEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEVE 50
Cdd:cd05363    1 LDGKTALITGSARGIGRAFAQAYVREGARvAIADINLEAARATAAEIG 48
PRK12939 PRK12939
short chain dehydrogenase; Provisional
 1-56 2.22e-03

short chain dehydrogenase; Provisional


Pssm-ID: 183833 [Multi-domain]  Cd Length: 250  Bit Score: 35.72  E-value: 2.22e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLA-DGQARALGKVDVEACGALVGEVEVWGRDV 56
Cdd:PRK12939   2 ASNLAGKRALVTGAARGLGAAFAEALAeAGATVAFNDGLAAEARELAAALEAAGGRA 58
PRK08643 PRK08643
(S)-acetoin forming diacetyl reductase;
5-72 2.30e-03

(S)-acetoin forming diacetyl reductase;


Pssm-ID: 181518 [Multi-domain]  Cd Length: 256  Bit Score: 35.86  E-value: 2.30e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489505128   5 EGKVAFITGVARGQGRSHAVRLA-DGQARALGKVDVEACGALVGEVEVWGR-------DVRDDRRVF--VESPADEFG 72
Cdd:PRK08643   1 MSKVALVTGAGQGIGFAIAKRLVeDGFKVAIVDYNEETAQAAADKLSKDGGkaiavkaDVSDRDQVFaaVRQVVDTFG 78
PRK12829 PRK12829
short chain dehydrogenase; Provisional
 1-86 2.73e-03

short chain dehydrogenase; Provisional


Pssm-ID: 183778 [Multi-domain]  Cd Length: 264  Bit Score: 35.42  E-value: 2.73e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQARalgkvdVEACGALVGEVEVWGRDVRDDRRVFVESPADEFGACRRVARQ 80
Cdd:PRK12829   6 LKPLDGLRVLVTGGASGIGRAIAEAFAEAGAR------VHVCDVSEAALAATAARLPGAKVTATVADVADPAQVERVFDT 79


                 ....*.
gi 489505128  81 GIRVVG 86
Cdd:PRK12829  80 AVERFG 85
DHRS1_HSDL2-like_SDR_c cd05338
human dehydrogenase/reductase (SDR family) member 1 (DHRS1) and human hydroxysteroid ...
 4-72 3.86e-03

human dehydrogenase/reductase (SDR family) member 1 (DHRS1) and human hydroxysteroid dehydrogenase-like protein 2 (HSDL2), classical (c) SDRs; This subgroup includes human DHRS1 and human HSDL2 and related proteins. These are members of the classical SDR family, with a canonical Gly-rich NAD-binding motif and the typical YXXXK active site motif. However, the rest of the catalytic tetrad is not strongly conserved. DHRS1 mRNA has been detected in many tissues, liver, heart, skeletal muscle, kidney and pancreas; a longer transcript is predominantly expressed in the liver , a shorter one in the heart. HSDL2 may play a part in fatty acid metabolism, as it is found in peroxisomes. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H)-binding pattern (typically, TGxxxGxG in classical SDRs and TGxxGxxG in extended SDRs), while substrate binding is in the C-terminal region. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr and Lys, as well as Asn (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.


Pssm-ID: 187597 [Multi-domain]  Cd Length: 246  Bit Score: 35.06  E-value: 3.86e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489505128   4 LEGKVAFITGVARGQGRSHAVRLA-DG-----QARALGKVDVEACGALVGEVEVWGR--------------DVRDDRRV- 62
Cdd:cd05338    1 LSGKVAFVTGASRGIGRAIALRLAkAGatvvvAAKTASEGDNGSAKSLPGTIEETAEeieaaggqalpivvDVRDEDQVr 80

                         90
                 ....*....|.
gi 489505128  63 -FVESPADEFG 72
Cdd:cd05338   81 aLVEATVDQFG 91
PRK12746 PRK12746
SDR family oxidoreductase;
1-31 7.48e-03

SDR family oxidoreductase;


Pssm-ID: 183718 [Multi-domain]  Cd Length: 254  Bit Score: 34.24  E-value: 7.48e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQA 31
Cdd:PRK12746   1 MKNLDGKVALVTGASRGIGRAIAMRLANDGA 31
PRK06057 PRK06057
short chain dehydrogenase; Provisional
 1-49 8.64e-03

short chain dehydrogenase; Provisional


Pssm-ID: 180371 [Multi-domain]  Cd Length: 255  Bit Score: 34.32  E-value: 8.64e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLADGQAR-ALGKVDVEACGALVGEV 49
Cdd:PRK06057   2 SQRLAGRVAVITGGGSGIGLATARRLAAEGATvVVGDIDPEAGKAAADEV 51
PRK08226 PRK08226
SDR family oxidoreductase UcpA;
1-23 9.05e-03

SDR family oxidoreductase UcpA;


Pssm-ID: 181305 [Multi-domain]  Cd Length: 263  Bit Score: 34.01  E-value: 9.05e-03
                         10        20
                 ....*....|....*....|...
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHA 23
Cdd:PRK08226   1 MGKLTGKTALITGALQGIGEGIA 23
fabG PRK07666
3-ketoacyl-(acyl-carrier-protein) reductase; Provisional
 1-27 9.99e-03

3-ketoacyl-(acyl-carrier-protein) reductase; Provisional


Pssm-ID: 236074 [Multi-domain]  Cd Length: 239  Bit Score: 33.89  E-value: 9.99e-03
                         10        20
                 ....*....|....*....|....*..
gi 489505128   1 MGRLEGKVAFITGVARGQGRSHAVRLA 27
Cdd:PRK07666   2 AQSLQGKNALITGAGRGIGRAVAIALA 28
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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